Intestinal regulation composition containing roxburgh rose extract and preparation method thereof

Abstract

The invention provides an intestinal regulation composition containing a roxburgh rose extract. The composition is prepared from the following raw materials in parts by weight: 1800-2100 parts of the roxburgh rose extract, 420-680 parts of perilla seeds, 420-680 parts of celery seeds, 770-850 parts of poria cocos, 770-850 parts of notopterygium roots, 770-850 parts of radish seeds, 220-280 parts of honey, 7-11 parts of saccharomycetes, 7-11 parts of lactobacillus acidophilus, 7-11 parts of bifidobacterium longum, 100-200 parts of lactoferrin powder, a proper amount of compound enzyme and a proper amount of water. The invention also provides a preparation method of the intestinal regulation composition containing the roxburgh rose extract. The intestinal regulation composition provided by the invention can effectively improve the digestive function, enhance the intestinal barrier and immunity, promote the balance of flora in the intestinal tract, promote the metabolism of the human body, and realize the comprehensive optimization of the intestinal function.

Description

Technical Field

[0001] The invention relates to the technical field of biological food, and in particular to an intestinal regulating composition containing a roxburgh roxburgh extract and a preparation method thereof. Background Art

[0002] With the changes in modern lifestyle and the unreasonable dietary structure, intestinal health issues are receiving increasing attention. The intestine is not only an important place for human digestion and absorption, but also the largest immune organ of the human body. Its microecological balance is crucial to maintaining overall health. Intestinal flora imbalance is closely related to the occurrence of many diseases, such as obesity, diabetes, cardiovascular disease, inflammatory bowel disease, etc. Therefore, the development of health products that can regulate intestinal function and improve intestinal microecology has important practical significance.

[0003] Rosa roxburghii is a wild plant unique to my country, and its fruit is rich in a variety of bioactive ingredients, such as vitamin C, polysaccharides, polyphenols, etc. Studies in recent years have shown that rosa roxburghii extract has a variety of health benefits, including antioxidant, hypoglycemic, anti-inflammatory, and regulation of intestinal microbiota. For example, rosa roxburghii polysaccharides can be used as a prebiotic to promote the proliferation of beneficial intestinal bacteria and inhibit the growth of harmful bacteria, thereby maintaining the balance of intestinal microecology. In addition, rosa roxburghii extract can also prevent and relieve intestinal diseases such as ulcerative colitis by regulating intestinal microbiota and related signaling pathways.

[0004] However, there are relatively few products based on natural plant extracts for intestinal regulation on the market. Therefore, developing an intestinal regulation composition with roxburgh extract as the main ingredient can not only enrich the types of intestinal regulation products, but also provide consumers with a more natural, safe and healthy choice. Summary of the invention

[0005] In view of the deficiencies in the prior art, the present invention provides an intestinal regulating composition containing roxburgh extract and a preparation method thereof, which can help regulate intestinal health, cooperate with probiotics in the human gastrointestinal tract to promote gastrointestinal absorption, and improve human immunity.

[0006] The above technical objectives of the present invention are achieved through the following technical solutions:

[0007] An intestinal regulating composition containing a roxburghii extract, the composition being prepared from the following raw materials in a weight ratio:

[0008] Rosa roxburghii extract 1800-2100, Perilla seed 420-680, Celery seed 420-680, Poria cocos 770-850, Notopterygium incisum 770-850, Radish seed 770-850, Honey 220-280, Yeast 7-11, Lactobacillus acidophilus 7-11, Bifidobacterium longum 7-11, Lactoferrin powder 100-200, appropriate amount of complex enzyme, appropriate amount of water.

[0009] Furthermore, the composition is prepared from the following raw materials in weight ratio:

[0010] Rosa roxburghii extract 1970, Perilla seed 530, Celery seed 530, Poria cocos 800, Notopterygium incisum 800, Radish seed 800, Honey 250, Yeast 9, Lactobacillus acidophilus 9, Bifidobacterium longum 9, Lactoferrin powder 150, appropriate amount of complex enzyme, appropriate amount of water.

[0011] The present invention also provides a method for preparing an intestinal regulating composition containing a roxburghii extract, comprising the following steps:

[0012] S1, washing and low-temperature drying of perilla seeds, celery seeds, Poria cocos, Notopterygium wilfordii and radish seeds, and then ultrafine grinding to obtain raw material powder; S2, mixing the raw material powder, Rosa roxburghii extract and an appropriate amount of water, adding a complex enzyme, treating in an ultrasonic dispersion device for 30-35 minutes, and then standing for 2-4 hours; S3, redispersing the product after standing in step S2 evenly, adding honey and yeast, and preliminarily fermenting at 28-35° C. for 8-12 hours; S4, redispersing the preliminary fermentation product in step S3 evenly, adjusting the pH of the fermentation liquid to 6.3-6.8, adding Lactobacillus acidophilus and Bifidobacterium longum, and secondary fermenting at 35-40° C. for 18-24 hours, and irradiating with a dynamic spectrum during the secondary fermentation; S5, redispersing the secondary fermentation product in step S4 evenly, and taking the filtrate after centrifugation; S6, spray-drying the filtrate obtained in step S5, adding lactoferrin powder and uniformly mixing, and finally obtaining a composite powder.

[0013] Furthermore, the preparation method of the roxburghii extract is:

[0014] First, fresh roxburgh fruits with a maturity of 80% to 90% are selected, and impurities on the surface of the roxburgh fruits are removed by washing with running water, and the fruit thorns are removed by brushing; the washed roxburgh fruits are sliced ​​to a thickness of 2-3 mm, and the slices are dried by hot air at 60-70° C. to a moisture content of ≤8%, and then crushed through a 40-mesh sieve to obtain roxburgh dry powder; the roxburgh dry powder is taken, 70% ethanol is added according to a material-liquid mass ratio of 1: (20-30), and reflux extraction is performed at 60° C. for 2-3 times, each time for 1-2 hours, to obtain an extract; the extracts obtained in the above steps are combined, concentrated under reduced pressure at 50° C., and finally a powdered roxburgh extract is obtained by spray drying.

[0015] Furthermore, in step S2, the complex enzyme is a mixture of cellulase and pectinase, and the mass ratio of cellulase to pectinase is set to 1:(1-1.3).

[0016] Further, in step S4, the dynamic spectrum is set to 660 nm for red light and 450 nm for blue light, and the light intensity is set to 200 μmol / ㎡ / s.

[0017] Furthermore, the nutritional composition is processed into a product suitable for consumption by adding auxiliary materials acceptable in food science.

[0018] Furthermore, the nutritional composition is processed into forms including but not limited to tablets, capsules, pills, granules, powders, and drinks.

[0019] The present invention has the following beneficial effects:

[0020] The intestinal regulating composition containing roxburghii extract provided by the present invention can effectively improve digestive function, promote constipation relief and diarrhea regulation; can enhance intestinal barrier and immunity, promote the balance of intestinal flora, and stimulate intestinal immune cell activation; can enhance intestinal antioxidant and anti-inflammatory protection, reduce intestinal oxidative stress damage and inhibit inflammatory factors; can promote human metabolism, which is beneficial to weight management. And the intestinal regulating composition achieves comprehensive optimization of intestinal function through the synergy of natural ingredients and innovative process technology, and has both high efficiency, safety and stability, and has excellent value of metabolic regulation and immune enhancement. The intestinal regulating composition of this scheme is processed into tablets, capsules, pills, granules, powders or other beverages, and the product form is diversified and suitable for consumption. DETAILED DESCRIPTION

[0021] The technical solution of the present invention is further described below in conjunction with embodiments and comparative examples.

[0022] Embodiment 1:

[0023] An intestinal regulating composition containing a roxburghii extract is prepared from the following raw materials in weight proportion: 1800 parts roxburghii extract, 420 parts perilla seeds, 420 parts celery seeds, 770 parts tuckahoe, 770 parts notopterygium wilfordii, 770 parts radish seeds, 220 parts honey, 7 parts yeast, 7 parts lactobacillus acidophilus, 7 parts bifidobacterium longum, 100 parts lactoferrin powder, an appropriate amount of complex enzyme, and an appropriate amount of water.

[0024] Embodiment 2:

[0025] An intestinal regulating composition containing a roxburgh roxburgh extract is prepared from the following raw materials in weight proportion: 2100 grams of roxburgh roxburgh extract, 680 grams of perilla seeds, 680 grams of celery seeds, 850 grams of tuckahoe, 850 grams of notopterygium root, 850 grams of radish seeds, 280 grams of honey, 11 grams of yeast, 11 grams of lactobacillus acidophilus, 11 grams of bifidobacterium longum, 200 grams of lactoferrin powder, an appropriate amount of complex enzyme, and an appropriate amount of water.

[0026] Embodiment 3:

[0027] An intestinal regulating composition containing a roxburgh roxburgh extract is prepared from the following raw materials in weight ratio: 1970 grams of roxburgh roxburgh extract, 530 grams of perilla seeds, 530 grams of celery seeds, 800 grams of tuckahoe, 800 grams of notopterygium root, 800 grams of radish seeds, 250 grams of honey, 9 grams of yeast, 9 grams of lactobacillus acidophilus, 9 grams of bifidobacterium longum, 150 grams of lactoferrin powder, an appropriate amount of complex enzyme, and an appropriate amount of water.

[0028] The preparation methods of the above embodiments 1, 2 and 3 include the following steps: S1, washing and low-temperature drying perilla seeds, celery seeds, Poria cocos, Notopterygium wilfordii and radish seeds, and then ultrafine grinding to obtain raw powder; S2, mixing the raw powder, roxburghii extract and an appropriate amount of water, wherein the mass ratio of the roxburghii extract to the added water is 1:10, and adding a composite enzyme, wherein the composite enzyme is a mixture of cellulase and pectinase in an equal mass ratio, and the amount of the composite enzyme added is 1% of the mass sum of the raw powder and the roxburghii extract, and then treating the above mixture in an ultrasonic dispersion device for 30 minutes, and then standing for 4 hours; S3, weighing the product after standing in step S2; The method comprises the following steps: re-dispersing the primary fermentation product in step S3 uniformly, adding honey and yeast, and performing preliminary fermentation at 35° C. for 12 hours; S4, re-dispersing the primary fermentation product in step S3 uniformly, adjusting the pH value of the fermentation liquid to 6.3, adding Lactobacillus acidophilus and Bifidobacterium longum, and performing secondary fermentation at 37° C. for 18 hours, and using dynamic spectrum for irradiation during the secondary fermentation, wherein the dynamic spectrum is set to 660 nm for red light and 450 nm for blue light, and the illumination intensity is set to 200 μmol / ㎡ / s; S5, re-dispersing the secondary fermentation product in step S4 uniformly, and taking the filtrate after centrifugation; S6, spray-drying the filtrate obtained in step S5, adding lactoferrin powder and mixing uniformly, and finally obtaining a composite powder.

[0029] The preparation method of the roxburghii extract comprises the following steps: firstly, selecting fresh roxburghii fruits with a maturity of 80% to 90%, preferably dark yellow fruits with a fruity aroma, washing with running water to remove impurities on the surface of the fresh roxburghii fruits, and brushing to remove the thorns; slicing the washed fresh roxburghii fruits, the slices having a thickness of 3 mm, drying by hot air at 60° C. to a moisture content of ≤8%, and then crushing through a 40-mesh sieve to obtain roxburghii dry powder; taking the roxburghii dry powder, adding 70% ethanol according to a material-liquid mass ratio of 1:20, reflux extraction at 60° C. for 3 times, each time for 2 hours, to obtain an extract; combining the extracts obtained in the above steps, concentrating under reduced pressure at 50° C., and finally obtaining a powdered roxburghii extract by spray drying.

[0030] Comparative Example 1:

[0031] An intestinal regulating composition is prepared from the following raw materials in weight ratio: 530 grams of perilla seeds, 530 grams of celery seeds, 800 grams of tuckahoe, 800 grams of notopterygium root, 800 grams of radish seeds, 250 grams of honey, 9 grams of yeast, 9 grams of lactobacillus acidophilus, 9 grams of bifidobacterium longum, 150 grams of lactoferrin powder, appropriate amount of complex enzyme, and appropriate amount of water.

[0032] The preparation method of comparative example 1 comprises the following steps: S1, washing and low-temperature drying perilla seeds, celery seeds, poria cocos, notopterygium wilfordii, and radish seeds, and then ultrafine grinding them to obtain raw material powder; S2, mixing the raw material powder with an appropriate amount of water, wherein the mass ratio of the raw material powder to the added water is 1:10, and adding a composite enzyme, wherein the composite enzyme is a mixture of cellulase and pectinase in an equal mass ratio, and the amount of the composite enzyme added is 1% of the mass of the raw material powder, and then treating the above mixture in an ultrasonic dispersion device for 30 minutes, and then standing for 4 hours; S3, redispersing the product after standing in step S2, adding honey and yeast, and mixing the mixture with water; The mother bacteria are preliminarily fermented at 35°C for 12 hours; S4, the preliminarily fermented product in step S3 is evenly redispersed, and the pH of the fermentation liquid is adjusted to 6.3, Lactobacillus acidophilus and Bifidobacterium longum are added, and secondary fermentation is carried out at 37°C for 18 hours. During the secondary fermentation, dynamic spectrum is used for irradiation, and the dynamic spectrum is set to red light 660nm, blue light 450nm, and the illumination intensity is set to 200μmol / ㎡ / s; S5, the secondary fermentation product in step S4 is evenly redispersed, and the filtrate is taken after centrifugation; S6, the filtrate obtained in step S5 is spray-dried, and then lactoferrin powder is added and evenly mixed to finally obtain a composite powder.

[0033] Comparative Example 2:

[0034] An intestinal regulating composition containing a roxburgh roxburgh extract is prepared from the following raw materials in weight ratio: 1970 grams of roxburgh roxburgh extract, 530 grams of perilla seeds, 530 grams of celery seeds, 800 grams of tuckahoe, 800 grams of notopterygium root, 800 grams of radish seeds, 250 grams of honey, 9 grams of yeast, 9 grams of lactobacillus acidophilus, 9 grams of bifidobacterium longum, 150 grams of lactoferrin powder, an appropriate amount of complex enzyme, and an appropriate amount of water.

[0035] The preparation method of comparative example 2 comprises the following steps: S1, washing and low-temperature drying perilla seeds, celery seeds, Poria cocos, Notopterygium wilfordii, and radish seeds, and then ultrafine grinding them to obtain raw material powder; S2, mixing the raw material powder, roxburghii extract and an appropriate amount of water, wherein the mass ratio of the roxburghii extract to the added water is 1:10, and adding a composite enzyme, wherein the composite enzyme is a mixture of cellulase and pectinase in an equal mass ratio, and the amount of the composite enzyme added is 1% of the mass of the raw material powder and the roxburghii extract, and then treating the above mixture in an ultrasonic dispersion device for 30 minutes. n, then let stand for 4h; S3, redisperse the product after standing in step S2, add honey and yeast, and ferment at 35°C for 12h; S4, redisperse the preliminary fermentation product in step S3, adjust the pH of the fermentation liquid to 6.3, add Lactobacillus acidophilus and Bifidobacterium longum, and ferment at 37°C for 18h; S5, redisperse the secondary fermentation product in step S4, and take the filtrate after centrifugation; S6, spray-dry the filtrate obtained in step S5, add lactoferrin powder and mix evenly, and finally obtain a composite powder. The preparation method of the roxburghii extract in this comparative example 2 is the same as that in Example 1-3.

[0036] Comparative Example 3:

[0037] An intestinal regulating composition containing a roxburgh roxburgh extract is prepared from the following raw materials in weight ratio: 1970 parts roxburgh roxburgh extract, 530 parts perilla seeds, 530 parts celery seeds, 800 parts tuckahoe, 800 parts notopterygium wilfordii, 800 parts radish seeds, 150 parts lactoferrin powder, an appropriate amount of complex enzyme, and an appropriate amount of water.

[0038] The preparation method of this comparative example 3 comprises the following steps: S1, taking perilla seeds, celery seeds, Poria cocos, Notopterygium wilfordii, and radish seeds, washing them, low-temperature drying them, and then ultrafine grinding them to obtain raw material powder; S2, mixing the raw material powder, the roxburghii extract and an appropriate amount of water, in this step, the mass ratio of the roxburghii extract to the added water is 1:10, and adding a composite enzyme, the composite enzyme is a mixture of cellulase and pectinase in an equal mass ratio, and the amount of the composite enzyme added is 1% of the mass sum of the raw material powder and the roxburghii extract, and then treating the above mixture in an ultrasonic dispersion device for 30 minutes, and then standing for 4 hours; S3, redispersing the product after standing in step S2 evenly, and taking the filtrate after centrifugation; S4, spray drying the filtrate obtained in step S3, adding lactoferrin powder and mixing evenly, and finally obtaining a composite powder. The preparation method of the roxburghii extract in this comparative example 3 is the same as that in Examples 1-3.

[0039] The differences between Examples 1-3 and Comparative Examples 1-3 are shown in the following table:

[0040]

[0041]

[0042] The beneficial effects of the present invention are further illustrated by comparative experiments below.

[0043] 1. Selection of experimental individuals

[0044] A number of healthy male SD rats aged 6-8 weeks were selected, and after being stably fed under the same conditions of 24-26°C and 40-50% humidity for 1 week, 70 rats weighing 200±10g were selected from them. The 70 rats were randomly divided into 7 experimental groups, marked as experimental groups 1-7, with 10 rats in each experimental group.

[0045] 2. Classification of experimental groups

[0046] The rats in the seven experimental groups continued to be fed in an environment with a temperature of 27-29°C and a humidity of 40-50%:

[0047] Experimental group 1: fed with basic feed and gavaged with the intestinal regulating composition prepared in Example 1;

[0048] Experimental group 2: The mice were fed with basic feed and intragastrically administered with the intestinal regulating composition prepared in Example 2;

[0049] Experimental group 3: The mice were fed with basic feed and intragastrically administered with the intestinal regulating composition prepared in Example 3;

[0050] Experimental group 4: fed with basic feed and gavaged with the intestinal regulating composition prepared in comparative example 1;

[0051] Experimental group 5: fed with basic feed and gavaged with the intestinal regulating composition prepared in comparative example 2;

[0052] Experimental group 6: fed with basic feed and gavaged with the intestinal regulating composition prepared in comparative example 3;

[0053] Experimental group 7: fed only with basal feed;

[0054] Among them, in experimental groups 1-6, the cycle of intragastric administration of the intestinal regulating composition was 28 consecutive days, with a dose of 200 mg / kg·d;

[0055] 3. Experimental methods and data:

[0056] 1. After the experimental period, the intestinal flora balance test (16S rRNA sequencing) was performed on the rats in experimental groups 1-7:

[0057]

[0058]

[0059] 2. After the experimental period, the rats in experimental groups 1-7 were tested for immunity indexes (serum testing)

[0060]

[0061] 4. Experimental conclusion:

[0062] The intestinal regulating composition of this scheme adopts a staged fermentation technology, which can effectively avoid bacterial competition, and the yeast metabolites can also provide growth substrates for probiotics, thereby improving the fermentation effect. At the same time, dynamic spectrum control is adopted during the fermentation process. Blue light can promote yeast metabolism, and red light can stimulate probiotics to produce extracellular polysaccharides, thereby accurately optimizing the bacterial metabolic pathway to improve the quality characteristics of the intestinal regulating composition proposed in this scheme.

[0063] The intestinal flora balance test results show that the intestinal regulating composition proposed in this scheme can work synergistically and further cooperate with the original probiotics in the intestine to improve the diversity of intestinal flora; in particular, the roxburghii extract can effectively promote the colonization of probiotics. At the same time, the intestinal regulating composition proposed in this scheme can also increase serum IgA and IgG levels, enhance immunity and promote body metabolism, which is beneficial to weight management.

[0064] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution of the present invention, which should be included in the scope of the claims of the present invention.

Claims

1. An intestinal regulating composition containing a roxburghii extract, characterized in that: The composition is prepared from the following raw materials in weight ratio: Rosa roxburghii extract 1800-2100, Perilla seed 420-680, Celery seed 420-680, Poria cocos 770-850, Notopterygium incisum 770-850, Radish seed 770-850, Honey 220-280, Yeast 7-11, Lactobacillus acidophilus 7-11, Bifidobacterium longum 7-11, Lactoferrin powder 100-200, appropriate amount of complex enzyme, appropriate amount of water.

2. The intestinal regulating composition containing the roxburghii extract according to claim 1, characterized in that: The composition is prepared from the following raw materials in weight ratio: Rosa roxburghii extract 1970, Perilla seed 530, Celery seed 530, Poria cocos 800, Notopterygium incisum 800, Radish seed 800, Honey 250, Yeast 9, Lactobacillus acidophilus 9, Bifidobacterium longum 9, Lactoferrin powder 150, appropriate amount of complex enzyme, appropriate amount of water.

3. A method for preparing an intestinal regulating composition containing a roxburghii extract according to any one of claims 1 to 2, characterized in that: The following steps are involved: S1, washing and low-temperature drying perilla seeds, celery seeds, Poria cocos, Notopterygium wilfordii, and radish seeds, and then ultrafine grinding to obtain raw material powder; S2, mixing the raw material powder, the roxburghii extract and an appropriate amount of water, adding a composite enzyme, treating in an ultrasonic dispersion device for 30-35 min, and then standing for 2-4 h; S3, redispersing the product after standing in step S2, adding honey and yeast, and preliminarily fermenting at 28-35° C. for 8-12 hours; S4, redispersing the preliminary fermentation product in step S3 uniformly, adjusting the pH of the fermentation liquid to 6.3-6.8, adding Lactobacillus acidophilus and Bifidobacterium longum, and performing secondary fermentation at 35-40° C. for 18-24 h, and irradiating with dynamic spectrum during the secondary fermentation; S5, redispersing the secondary fermentation product in step S4 uniformly, and taking the filtrate after centrifugation; S6, spray-drying the filtrate obtained in step S5, adding lactoferrin powder and mixing evenly to finally obtain a composite powder.

4. The method for preparing an intestinal regulating composition containing a roxburghii extract according to claim 3, characterized in that: The preparation method of the roxburghii extract is: Firstly, fresh roxburghii fruits with a maturity of 80% to 90% are selected, and impurities on the surface of the roxburghii fruits are removed by washing with running water, and the fruit thorns are removed by brushing; The washed fresh roxburghii fruit is sliced ​​to a thickness of 2-3 mm, dried by hot air at 60-70° C. to a moisture content of ≤8%, and then crushed through a 40-mesh sieve to obtain roxburghii dry powder; Take the dried Rosa roxburghii powder, add 70% ethanol in a material-liquid mass ratio of 1:(20-30), and reflux extract at 60° C. for 2-3 times, each time for 1-2 hours, to obtain an extract; The extracts obtained in the above steps were combined, concentrated under reduced pressure at 50° C., and finally spray-dried to obtain a powdered roxburghii extract.

5. The method for preparing an intestinal regulating composition containing a roxburghii extract according to claim 3, characterized in that: In step S2, the complex enzyme is a mixture of cellulase and pectinase, and the mass ratio of cellulase to pectinase is set to 1:(1-1.3).

6. The method for preparing an intestinal regulating composition containing a roxburghii extract according to claim 3, characterized in that: In step S4, the dynamic spectrum is set to 660 nm for red light and 450 nm for blue light, and the light intensity is set to 200 μmol / ㎡ / s.

7. An intestinal regulating composition containing a roxburghii extract according to claims 1-2, characterized in that: The nutritional composition is processed into a product suitable for consumption by adding auxiliary materials acceptable to food science.

8. The intestinal regulating composition containing the roxburghii extract according to claim 7, characterized in that: The nutritional composition is processed into forms including but not limited to tablets, capsules, pills, granules, powders, and drinks.

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