Ginseng essence vitality-strengthening paste containing various medicinal materials and preparation method of ginseng essence vitality-strengthening paste

Abstract

The invention provides ginseng-essence vitality-consolidating paste containing various medicinal materials and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The ginseng essence vitality strengthening cream comprises hippocampus, penis et testis cervi, cistanche deserticola, rehmannia glutinosa, mulberry, black sesame, black beans, lycium ruthenicum, perigord truffle, oyster peptide powder, eucommia ulmoides male pollen, cinnamon, ginseng, polygonatum kingianum, donkey-hide gelatin, spina date seed, lily, citron, angelica sinensis, gamma-aminobutyric acid and isomaltooligosacharide. Wherein the hippocampus, the penis cervi, the cistanche and the rehmannia serve as monarch drugs; mulberry, black sesame, black beans, lycium ruthenicum, perigord truffle, oyster peptide powder, eucommia male pollen and cinnamon are used as ministerial drugs; ginseng, rhizoma polygonati, colla corii asini, spina date seed, lily, citron and angelica sinensis are used as adjuvant drugs. The medicines in the whole formula are combined to tonify the liver and the kidney and help the spleen and the stomach, so that the effects of regulating essence, spirit, qi and blood, improving vitality, strengthening tendons and bones, refreshing and restoring consciousness, blackening and brightening hair are achieved, and symptoms such as insomnia, anxiety, fatigue, impotence, premature ejaculation and the like caused by heart-spleen deficiency are relieved.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a ginseng essence and solidifying essence paste containing multiple medicinal materials and a preparation method thereof. Background Art

[0002] In today's fast-paced modern society, people are facing unprecedented life and work pressure, and various health problems derived from this are becoming increasingly prominent. Modern society is highly competitive, and work pressure has become the norm in people's lives. In order to complete their work tasks, many professionals have to work long hours and work overtime frequently. This work mode seriously disrupts the normal rhythm of life, making it difficult for the organs of the body to get enough rest and recovery. At the same time, irregular diet is also a common problem. Due to busy work, people often have no time to take care of a reasonable diet, often miss normal meals, or choose fast food, take-out and other convenient but nutritious foods. In the long run, the spleen and stomach functions are greatly affected, and it is difficult to transport and transform the essence of water and grain normally and provide sufficient nutritional support for the body. Excessive overtime work not only directly leads to physical fatigue, but also seriously affects the quality of sleep. Sleep is an important period for the human body to repair and adjust itself. Poor sleep will prevent the body's various functions from being effectively restored, and then affect the normal operation of multiple physiological systems such as the immune system and endocrine system. The heart governs the spirit, and the spleen is the foundation of the acquired constitution and the source of qi and blood biochemistry. Long-term work pressure, improper diet and poor sleep can easily damage the qi of the heart and spleen. Deficiency of both the heart and spleen can cause a series of health problems, such as palpitations, insomnia, nightmares, loss of appetite, abdominal distension, loose stools, fatigue, sallow complexion, etc., which seriously affect people's quality of life and work efficiency.

[0003] Traditional medicine believes that the health of the human body depends on the sufficiency of qi and blood and the coordination of the functions of the internal organs. The heart and spleen play a vital role in the life activities of the human body. The heart governs the blood vessels, promoting the blood to flow through the veins and nourishing the whole body; the spleen governs transportation and transformation, transforming water and grain into fine substances and transporting them to all parts of the body. Only when the heart and spleen function normally can the human body be full of energy and vitality. However, the damage to the heart and spleen caused by modern lifestyles threatens the overall health of the human body.

[0004] At present, there are many solutions for such health problems on the market, but most of them have limitations. Although some drugs can relieve symptoms in the short term, they may bring side effects, and long-term use may also cause other damage to the body. Some ordinary nutritional supplements are often unable to fundamentally regulate the heart and spleen functions, and it is difficult to achieve the effect of comprehensively improving vitality and strengthening the body. Therefore, there is an urgent need to develop a safe and effective product to improve the problem of heart and spleen deficiency caused by modern lifestyles and enhance human vitality. Summary of the invention

[0005] In view of the above technical problems, the purpose of the present invention is to provide a ginseng essence and solidifying essence paste containing multiple medicinal materials and a preparation method thereof. The ginseng essence and solidifying essence paste provided by the present invention can relieve symptoms such as anxiety, insomnia, impotence and premature ejaculation caused by deficiency of both heart and spleen, ensure the normal essence, spirit, qi and blood of the human body, enhance vitality, strengthen muscles and bones, refresh the mind, and make hair black and shiny.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The invention provides a ginseng essence and solidifying essence paste containing multiple medicinal materials, comprising the following raw materials in parts by weight: 8-12 parts of seahorse, 8-12 parts of deer whip, 8-12 parts of Cistanche deserticola, 8-12 parts of Rehmannia root, 8-12 parts of mulberry, 8-12 parts of black sesame, 8-12 parts of black bean, 8-12 parts of black wolfberry, 3-7 parts of black truffle, 8-12 parts of oyster, 3-7 parts of Eucommia male pollen, 8-12 parts of cinnamon, 8-12 parts of ginseng, 8-12 parts of polygonatum, 3-7 parts of donkey-hide gelatin, 8-12 parts of spiny jujube seeds, 8-12 parts of lily, 8-12 parts of citron and 8-12 parts of angelica.

[0008] Preferably, γ-aminobutyric acid and isomaltooligosaccharide are also included.

[0009] The present invention also provides a preparation method of the above-mentioned kidney essence solidifying paste, comprising: drying and crushing all raw materials, mixing and heating seahorse, deer whip, oyster and water, adding composite enzyme for ultrasonic enzymolysis, filtering, spray drying the enzymolysis solution to obtain animal source powder; mixing rehmannia root, eucommia male pollen, cinnamon bark, ginseng, polygonatum, angelica and water, inoculating composite bacterial agent for fermentation, filtering, spray drying the fermentation solution to obtain fermentation powder; mixing mulberry, black sesame, black bean, black wolfberry, black truffle and ethanol solution, ultrasonic extraction, filtering, spray drying the extract solution to obtain ethanol extraction powder; mixing Cistanche deserticola, Ziziphus jujuba, lily, citron and water, heating extraction, filtering, concentrating to obtain extraction concentrate; mixing the extraction concentrate, donkey-hide gelatin, animal source powder, fermentation powder and ethanol extraction powder, heating and stirring until flagging, to obtain the kidney essence solidifying paste.

[0010] Preferably, the crushed particle size is 80-500 mesh.

[0011] Preferably, the composite enzyme is composed of flavor protease, snail protease and bromelain in a weight ratio of (1-3):1:1; the added amount of the composite enzyme is 1%-3% of the total mass of the seahorse, deer whip and oyster; the frequency of the ultrasonic enzymolysis is 20-40kHz, the temperature is 40-50°C, the pH value is 6.5-7.5, and the time is 2-5h.

[0012] Preferably, the composite bacterial agent is composed of Saccharomyces boulardii, Lactobacillus rhamnosus and Bifidobacterium longum subspecies longum in a weight ratio of (1-3):(1-3):(1-3); the inoculation amount of the composite bacterial agent is 3%-8% of the total mass of the Rehmannia glutinosa, Eucommia ulmoides male pollen, cinnamon bark, ginseng, Polygonatum sibiricum and Angelica sinensis, the temperature is 35-40°C, the pH value is 6.5-7.5, and the time is 2-5d.

[0013] Preferably, the volume percentage of the ethanol solution is 75%-85%; the material-liquid ratio of the ultrasonic extraction is 1:5-10 g / mL, the frequency is 40-60 kHz, the temperature is 55-65°C, the number of times is 1-3 times, each time for 20-30 minutes.

[0014] Preferably, the solid-liquid ratio of the heating extraction is 1:4-8 g / mL, the temperature is 90-100° C., the number of times is 2-4 times, and each time is 30-40 minutes.

[0015] Preferably, the temperature of the heating and stirring is 90-100°C.

[0016] The present invention also provides the use of the ginseng essence and solidifying essence paste in preparing a medicine for alleviating symptoms of deficiency of both heart and spleen.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The invention provides a ginseng essence and solidifying essence paste containing multiple medicinal materials, including: seahorse, deer whip, Cistanche deserticola, rehmannia root, mulberry, black sesame, black bean, black wolfberry, black truffle, oyster peptide powder, eucommia male pollen, cinnamon, ginseng, polygonatum, donkey-hide gelatin, spiny jujube seed, lily, citron and angelica. Among them, seahorse, deer whip, Cistanche deserticola and Rehmannia root are the main drugs, which warm and nourish kidney yang, replenish essence and marrow, nourish yin and moisten intestines, so as to enhance liver and kidney essence, make people refreshed and radiant; mulberry, black sesame, black bean, black wolfberry, black truffle, oyster peptide powder, Eucommia male pollen and cinnamon are the assistant drugs, nourish liver and kidney, warm yang and consolidate, and regulate human energy and hair quality by warming yang and benefiting kidney, nourishing liver and spleen; ginseng, polygonatum, donkey-hide gelatin, spiny jujube seed, lily, citron and angelica are the assistant drugs, which strengthen spleen and stomach, soothe liver and regulate qi, calm the mind and calm the nerves, and transform qi and blood to help the main drugs. γ-aminobutyric acid supplements human inhibitory neurotransmitters, strengthens brain and improves intelligence, promotes brain activation and promotes sleep; isomalt oligosaccharide harmonizes the taste and properties of the medicine. The idea of ​​the present invention is derived from the key to the secret recipe of the palace. It has strong efficacy without side effects, conforms to the current material of medicine and food of the same origin, and is composed of the Chinese medicine thinking, ministers, assistants and messengers. All the medicines in the whole recipe are used together, and the liver and kidney are nourished and the spleen and stomach are coordinated, so as to achieve the effects of harmonizing the essence, spirit, qi and blood, enhancing vitality, strengthening muscles and bones, refreshing the mind, and making hair black and shiny, and relieving symptoms such as insomnia, anxiety, fatigue, impotence and premature ejaculation caused by deficiency of both heart and spleen. DETAILED DESCRIPTION

[0019] The invention provides a ginseng essence and solidifying essence paste containing multiple medicinal materials, comprising the following raw materials in parts by weight: 8-12 parts of seahorse, 8-12 parts of deer whip, 8-12 parts of Cistanche deserticola, 8-12 parts of Rehmannia root, 8-12 parts of mulberry, 8-12 parts of black sesame, 8-12 parts of black bean, 8-12 parts of black wolfberry, 3-7 parts of black truffle, 8-12 parts of oyster, 3-7 parts of Eucommia male pollen, 8-12 parts of cinnamon, 8-12 parts of ginseng, 8-12 parts of polygonatum, 3-7 parts of donkey-hide gelatin, 8-12 parts of spiny jujube seeds, 8-12 parts of lily, 8-12 parts of citron and 8-12 parts of angelica.

[0020] The invention also comprises 1-3 parts of gamma-aminobutyric acid and 4-8 parts of isomaltooligosaccharide. The isomaltooligosaccharide is purchased from Baolingbao Biological Co., Ltd.

[0021] The liver and kidney are of the same origin. They nourish each other and produce essence and blood. Liver blood depends on the nourishment of kidney essence, and kidney essence depends on the supplement of liver blood. Liver blood and kidney essence nourish each other and transform into each other. Essence and blood are transformed from the essence of water and grain digested and absorbed by the spleen and stomach to maintain the dynamic balance of liver and kidney to ensure the normal essence, spirit, qi, blood, etc. of the human body. Therefore, the prescription uses the main medicines of seahorse, deer whip, Cistanche deserticola and Rehmannia glutinosa to warm and nourish kidney yang, replenish essence and fill marrow, nourish yin and moisten intestines, so as to enhance the essence of liver and kidney, make people refreshed and radiant. The liver stores blood and the kidney stores essence. The liver is responsible for dredging and smoothing. If the liver qi is stagnant, the heart will be upset and the spirit will be depressed; the kidney stores essence, the kidney controls water, regulates water and controls reproduction. The kidney is the root of the innate, the nail is the depth of the liver, the kidney controls the bones, the hair is the surplus of the kidney, and the nail is the surplus of blood. If the kidney essence is strong, the energy will be strong, the waist and knees will be strong, the urine will be clear, and the sleep will be sufficient. Therefore, the prescription uses the ministerial drugs mulberry, black sesame, black bean, black wolfberry, black truffle, oyster peptide powder, Eucommia male pollen, cinnamon to nourish the liver and kidney, warm the yang and consolidate the body, and regulate the energy and hair quality of the human body by warming the yang and benefiting the kidney, nourishing the liver and spleen. The spleen and kidney are both responsible for qi. If the qi is unobstructed, the congenital and acquired qi will be full, the human body will have vigorous qi and blood, and the spirit will be refreshed. Therefore, the prescription uses the adjuvant drugs ginseng, polygonatum, donkey-hide gelatin, jujube seed, lily, citron, and angelica to strengthen the spleen and stomach, soothe the liver and regulate qi, calm the mind and calm the nerves, and transform qi and blood to help the monarch drug. γ-aminobutyric acid supplements the inhibitory neurotransmitters of the human body, strengthens the brain and improves intelligence, promotes brain activation, and promotes sleep; isomalt oligosaccharide harmonizes the taste and properties of the medicine. The idea of ​​the present invention is derived from the key to the secret recipe of the palace. It has strong efficacy without side effects, conforms to the current material of medicine and food of the same origin, and is composed of the Chinese medicine thinking, ministers, assistants and messengers. All the medicines in the whole recipe are used together, and the liver and kidney are nourished and the spleen and stomach are coordinated, so as to achieve the effects of harmonizing the essence, spirit, qi and blood, enhancing vitality, strengthening muscles and bones, refreshing the mind, and making hair black and shiny, and relieving symptoms such as insomnia, anxiety, fatigue, impotence and premature ejaculation caused by deficiency of both heart and spleen.

[0022] The present invention also provides a preparation method of the above-mentioned kidney essence solidifying paste, comprising: drying and crushing all raw materials, mixing and heating seahorse, deer whip, oyster and water, adding composite enzyme for ultrasonic enzymolysis, filtering, spray drying the enzymolysis solution to obtain animal source powder; mixing rehmannia root, eucommia male pollen, cinnamon bark, ginseng, polygonatum, angelica and water, inoculating composite bacterial agent for fermentation, filtering, spray drying the fermentation solution to obtain fermentation powder; mixing mulberry, black sesame, black bean, black wolfberry, black truffle and ethanol solution, ultrasonic extraction, filtering, spray drying the extract solution to obtain ethanol extraction powder; mixing Cistanche deserticola, Ziziphus jujuba, lily, citron and water, heating extraction, filtering, concentrating to obtain extraction concentrate; mixing the extraction concentrate, donkey-hide gelatin, animal source powder, fermentation powder and ethanol extraction powder, heating and stirring until flagging, to obtain the kidney essence solidifying paste.

[0023] The particle size of the crushed material of the present invention is 80-500 mesh, the particle size of the crushed material other than donkey-hide gelatin is preferably 300-500 mesh, more preferably 400 mesh, and the crushed particle size of donkey-hide gelatin is 80-120 mesh. The raw material is crushed, the particle size becomes smaller, the specific surface area is significantly increased, the cells are broken, and more effective components can directly contact the extraction solvent, thereby improving the extraction efficiency.

[0024] The composite enzyme of the present invention is preferably composed of flavor protease, snail protease and bromelain in a weight ratio of (1-3): 1:1, more preferably 2:1:1; the enzyme activity of the flavor protease is preferably 200,000-300,000 U / g, more preferably 250,000 U / g; the enzyme activity of the snail protease is preferably 100,000-200,000 U / g, more preferably 150,000 U / g; the enzyme activity of the bromelain is preferably 80,000-120,000 U / g, more preferably 100,000 U / g. The addition amount of the composite enzyme is preferably 1%-3% of the total mass of the seahorse, deer whip and oyster, more preferably 2%; the frequency of the ultrasonic enzymolysis is preferably 20-40kHz, more preferably 30kHz, the temperature is preferably 40-50°C, more preferably 45°C, the pH value is preferably 6.5-7.5, more preferably 7, and the time is preferably 2-5h, more preferably 3h. Flavor protease, snail protease and bromelain have different cleavage sites, which can hydrolyze the protein of the substrate from different sites, improve the hydrolysis efficiency, produce more and smaller peptides and amino acids, which are more conducive to human absorption, and can improve the unpleasant odor of the substrate itself. After enzymatic hydrolysis, the flavor is richer and more coordinated, and the odor is reduced.

[0025] The composite bacterial agent of the present invention is composed of Saccharomyces boulardii, Lactobacillus rhamnosus and Bifidobacterium longum subsp. Longum according to a weight ratio of (1-3):(1-3):(1-3); the Saccharomyces boulardii was purchased from Xi'an Jiahe Biotechnology Co., Ltd. with a product number of JH-0815 and a bacterial activity of 18-22 billion cfu / g; the Lactobacillus rhamnosus was purchased from Shaanxi Dennis Biotechnology Co., Ltd. with a product number of DNS-SLTRGJ and a bacterial activity of 9-11 billion cfu / g; the long subsp. of Bifidobacterium longum is Bifidobacterium longum subsp. Longum CICC ® 24935, purchased from China Industrial Microbiological Culture Collection Management Center, with a bacterial activity of 5-7 billion cfu / g; the inoculation amount of the composite bacterial agent is preferably 3%-8% of the total mass of the rehmannia root, eucommia male pollen, cinnamon bark, ginseng, polygonatum, and angelica, more preferably 5%, the temperature is preferably 35-40°C, more preferably 37°C, the pH value is preferably 6.5-7.5, more preferably 7, and the time is preferably 2-5d, more preferably 4d.

[0026] The volume percentage of the ethanol solution of the present invention is preferably 75%-85%, more preferably 80%; the solid-liquid ratio of the ultrasonic extraction is preferably 1:5-10 g / mL, more preferably 1:8 g / mL, the frequency is preferably 40-60 kHz, more preferably 50 kHz, the temperature is preferably 55-65°C, more preferably 60°C, the number of times is preferably 1-3 times, more preferably 2 times, each time is preferably 20-30 min, more preferably 25 min.

[0027] The solid-liquid ratio of the heating extraction of the present invention is preferably 1:4-8 g / mL, more preferably 1:5 g / mL, the temperature is preferably 90-100°C, more preferably 95°C, the number of times is preferably 2-4 times, more preferably 3 times, each time is preferably 30-40 min, more preferably 35 min.

[0028] The temperature of the heating and stirring in the present invention is preferably 90-100°C, more preferably 95°C.

[0029] The method for taking the Kidney Essence Strengthening Origin Paste of the present invention is: take 10g of the paste on an empty stomach every morning, once a day. The paste is not suitable for people with cold, fever, weak spleen and stomach, and indigestion.

[0030] In the present invention, unless otherwise specified, all raw material components are commercially available products well known to those skilled in the art.

[0031] The technical solutions in the present invention will be described clearly and completely below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] In the following embodiments, unless otherwise specified, all of them are conventional methods.

[0033] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0034] Example 1

[0035] (1) Raw material preparation

[0036] Weigh 10 parts by weight of seahorse, 10 parts by weight of deer whip, 10 parts by weight of Cistanche deserticola, 10 parts by weight of Rehmannia root, 10 parts by weight of mulberry, 10 parts by weight of black sesame, 10 parts by weight of black bean, 10 parts by weight of black wolfberry, 5 parts by weight of black truffle, 10 parts by weight of oyster, 5 parts by weight of Eucommia male pollen, 10 parts by weight of cinnamon bark, 10 parts by weight of ginseng, 10 parts by weight of polygonatum, 5 parts by weight of donkey-hide gelatin, 10 parts by weight of spiny jujube seed, 10 parts by weight of lily, 10 parts by weight of citron, 10 parts by weight of angelica, 2 parts by weight of gamma-aminobutyric acid and 5 parts by weight of isomaltooligosaccharide, grind the raw materials except donkey-hide gelatin, gamma-aminobutyric acid and isomaltooligosaccharide, pass through a 400-mesh sieve, grind the donkey-hide gelatin and pass through a 100-mesh sieve, and soak it with an equal weight of rice wine for 24 hours to obtain a colloidal solution;

[0037] (2) Enzymatic hydrolysis

[0038] The seahorse, deer whip, oyster and water were mixed at a solid-liquid ratio of 1:8 g / mL, heated at 100°C for 15 min, cooled to 45°C, added with complex enzyme, and subjected to ultrasonic enzymolysis for 3 h at a frequency of 30 kHz, a temperature of 45°C and a pH value of 7, and the enzyme was inactivated at 85°C for 15 min, filtered, and the enzymolysis solution was spray-dried to obtain an animal-derived powder;

[0039] The compound enzyme is composed of flavor protease, snail protease and bromelain in a weight ratio of 2:1:1, the flavor protease activity is 250,000 U / g, the snail protease activity is 150,000 U / g, and the bromelain activity is 100,000 U / g; the addition amount of the compound enzyme is 2% of the total weight of the seahorse, deer whip and oyster;

[0040] The inlet air temperature of the spray drying is 125°C and the outlet air temperature is 75°C;

[0041] (3) Fermentation

[0042] Rehmannia root, Eucommia ulmoides male pollen, cinnamon bark, ginseng, polygonatum sibiricum, angelica sinensis and water were mixed at a solid-liquid ratio of 1:8 g / mL, the composite bacterial agent was inoculated, and anaerobically fermented for 4 days at 37°C and pH 7, filtered, and the fermented liquid was spray-dried to obtain a fermentation powder;

[0043] The composite bacterial agent is composed of Saccharomyces boulardii, Lactobacillus rhamnosus and Bifidobacterium longum subsp. longum in a weight ratio of 2:2:2; the activity of Saccharomyces boulardii is 20 billion cfu / g; the activity of Lactobacillus rhamnosus is 10 billion cfu / g; the activity of Bifidobacterium longum subsp. longum is 10 billion cfu / g; the inoculation amount of the composite bacterial agent is 5% of the total weight of Rehmannia glutinosa, Eucommia ulmoides male pollen, cinnamon bark, ginseng, Polygonatum sibiricum and Angelica sinensis;

[0044] The inlet air temperature of the spray drying is 125°C and the outlet air temperature is 75°C;

[0045] (4) Ethanol extraction

[0046] Mulberry, black sesame, black bean, black wolfberry, black truffle and 80% ethanol solution by volume were mixed at a solid-liquid ratio of 1:8 g / mL, ultrasonic extraction was performed at a frequency of 50 kHz and a temperature of 60°C for 25 min, and the residue was mixed with 80% ethanol solution by volume at a solid-liquid ratio of 1:8 g / mL, ultrasonic extraction was performed at a frequency of 50 kHz and a temperature of 60°C for 25 min, and the residue was filtered. The filtrate was combined and spray-dried to obtain an ethanol extraction powder;

[0047] The inlet air temperature of the spray drying is 125°C and the outlet air temperature is 75°C;

[0048] (5) Water extraction

[0049] Mix Cistanche deserticola, Ziziphus jujuba seeds, lily, citron and water at a solid-liquid ratio of 1:5 g / mL, heat and extract at 95°C for 35 min, filter, repeat the above extraction process twice on the residue, combine the filtrate, and concentrate at 70°C to 1 / 4 of the filtrate volume to obtain an extract concentrate;

[0050] (6) Mixing

[0051] The extract concentrate, colloidal solution, animal source powder, fermentation powder and ethanol extract powder, γ-aminobutyric acid and isomaltooligosaccharide are mixed and heated and stirred at 95° C. until the mixture is flagged, thereby obtaining the Kidney Essence Strengthening Ointment.

[0052] Example 2

[0053] (1) Raw material preparation

[0054] Weigh 8 parts by weight of seahorse, 8 parts by weight of deer whip, 8 parts by weight of Cistanche deserticola, 8 parts by weight of Rehmannia root, 8 parts by weight of mulberry, 8 parts by weight of black sesame, 8 parts by weight of black bean, 8 parts by weight of black wolfberry, 3 parts by weight of black truffle, 8 parts by weight of oyster, 3 parts by weight of Eucommia male pollen, 8 parts by weight of cinnamon bark, 8 parts by weight of ginseng, 8 parts by weight of polygonatum, 3 parts by weight of donkey-hide gelatin, 8 parts by weight of spiny jujube seed, 8 parts by weight of lily, 8 parts by weight of citron, 8 parts by weight of angelica, 1 part by weight of γ-aminobutyric acid and 4 parts by weight of isomaltooligosaccharide, grind the raw materials except donkey-hide gelatin, γ-aminobutyric acid and isomaltooligosaccharide, and pass through a 300-mesh sieve; grind the donkey-hide gelatin through an 80-mesh sieve, and soak it with an equal weight of rice wine for 24 hours to obtain a colloidal solution;

[0055] (2) Enzymatic hydrolysis

[0056] The seahorse, deer whip, oyster and water were mixed at a solid-liquid ratio of 1:5 g / mL, heated at 100°C for 10 min, cooled to 40°C, added with complex enzyme, and ultrasonically hydrolyzed for 5 h at a frequency of 20 kHz, a temperature of 40°C and a pH value of 6.5, and the enzyme was inactivated at 80°C for 20 min, filtered, and the enzymatic solution was spray-dried to obtain an animal-derived powder;

[0057] The compound enzyme is composed of flavor protease, snail protease and bromelain in a weight ratio of 2:1:1, the flavor protease activity is 200,000 U / g, the snail protease activity is 100,000 U / g, and the bromelain activity is 80,000 U / g; the addition amount of the compound enzyme is 1% of the total mass of the seahorse, deer whip and oyster;

[0058] The inlet air temperature of the spray drying is 120°C and the outlet air temperature is 70°C;

[0059] (3) Fermentation

[0060] Rehmannia root, Eucommia ulmoides male pollen, cinnamon bark, ginseng, polygonatum sibiricum, angelica sinensis and water were mixed at a solid-liquid ratio of 1:5 g / mL, the composite bacterial agent was inoculated, and anaerobically fermented for 5 days at 35°C and pH 6.5, filtered, and the fermented liquid was spray-dried to obtain a fermentation powder;

[0061] The composite bacterial agent is composed of Saccharomyces boulardii, Lactobacillus rhamnosus and Bifidobacterium longum subsp. longum in a weight ratio of 3:1:1; the activity of Saccharomyces boulardii is 18 billion cfu / g; the activity of Lactobacillus rhamnosus is 9 billion cfu / g; the activity of Bifidobacterium longum subsp. longum is 5 billion cfu / g; the inoculation amount of the composite bacterial agent is 3% of the total weight of Rehmannia glutinosa, Eucommia ulmoides male pollen, cinnamon bark, ginseng, Polygonatum sibiricum and Angelica sinensis;

[0062] The inlet air temperature of the spray drying is 120°C and the outlet air temperature is 70°C;

[0063] (4) Ethanol extraction

[0064] Mulberry, black sesame, black bean, black wolfberry, black truffle and 75% ethanol solution by volume were mixed at a solid-liquid ratio of 1:5 g / mL, and ultrasonically extracted for 20 min at a frequency of 60 kHz and a temperature of 55°C, filtered, and the above extraction process was repeated twice on the residue, the filtrate was combined, and the filtrate was spray-dried to obtain an ethanol extraction powder;

[0065] The inlet air temperature of the spray drying is 120°C and the outlet air temperature is 70°C;

[0066] (5) Water extraction

[0067] Mix Cistanche deserticola, Ziziphus jujuba seeds, Lilium, Citron and water at a solid-liquid ratio of 1:4 g / mL, heat and extract at 90°C for 30 min, filter, repeat the above extraction process for 3 times on the residue, combine the filtrate, and concentrate at 65°C to 1 / 5 of the filtrate volume to obtain an extract concentrate;

[0068] (6) Mixing

[0069] The extract concentrate, colloidal solution, animal source powder, fermentation powder and ethanol extract powder, γ-aminobutyric acid and isomaltooligosaccharide are mixed and heated and stirred at 90° C. until the mixture is flagged, thereby obtaining the Kidney Essence Strengthening Ointment.

[0070] Example 3

[0071] (1) Weigh 12 parts by weight of seahorse, 12 parts by weight of deer whip, 12 parts by weight of Cistanche deserticola, 12 parts by weight of Rehmannia root, 12 parts by weight of mulberry, 12 parts by weight of black sesame, 12 parts by weight of black bean, 12 parts by weight of black wolfberry, 7 parts by weight of black truffle, 12 parts by weight of oyster, 7 parts by weight of Eucommia male pollen, 12 parts by weight of cinnamon bark, 12 parts by weight of ginseng, 12 parts by weight of polygonatum, 7 parts by weight of donkey-hide gelatin, 12 parts by weight of spiny jujube seed, 12 parts by weight of lily, 12 parts by weight of citron, 12 parts by weight of angelica, 3 parts by weight of γ-aminobutyric acid and 8 parts by weight of isomaltooligosaccharide, grind the raw materials except donkey-hide gelatin, γ-aminobutyric acid and isomaltooligosaccharide, pass through a 500-mesh sieve, grind the donkey-hide gelatin and pass through a 120-mesh sieve, and soak it with an equal weight of rice wine for 24 hours to obtain a colloidal solution;

[0072] (2) Enzymatic hydrolysis

[0073] The seahorse, deer whip, oyster and water were mixed at a solid-liquid ratio of 1:10 g / mL, heated at 100°C for 20 min, cooled to 50°C, added with complex enzyme, and subjected to ultrasonic enzymolysis for 2 h at a frequency of 40 kHz, a temperature of 50°C and a pH value of 7.5, and the enzyme was inactivated at 90°C for 10 min, filtered, and the enzymolysis solution was spray-dried to obtain an animal-derived powder;

[0074] The compound enzyme is composed of flavor protease, snail protease and bromelain in a weight ratio of 1:1:1, the flavor protease activity is 300,000 U / g, the snail protease activity is 200,000 U / g, and the bromelain activity is 120,000 U / g; the addition amount of the compound enzyme is 3% of the total weight of the seahorse, deer whip and oyster;

[0075] The inlet air temperature of the spray drying is 130°C and the outlet air temperature is 80°C;

[0076] (3) Fermentation

[0077] Rehmannia root, Eucommia ulmoides male pollen, cinnamon bark, ginseng, polygonatum sibiricum, angelica sinensis and water were mixed at a solid-liquid ratio of 1:10 g / mL, the composite bacterial agent was inoculated, and anaerobically fermented for 3 days at 40°C and pH 7.5, filtered, and the fermented liquid was spray-dried to obtain a fermentation powder;

[0078] The composite bacterial agent is composed of Saccharomyces boulardii, Lactobacillus rhamnosus and Bifidobacterium longum subsp. longum in a weight ratio of 1:3:3; the activity of Saccharomyces boulardii is 22 billion cfu / g; the activity of Lactobacillus rhamnosus is 11 billion cfu / g; the activity of Bifidobacterium longum subsp. longum is 7 billion cfu / g; the inoculation amount of the composite bacterial agent is 8% of the total weight of Rehmannia glutinosa, Eucommia ulmoides male pollen, cinnamon bark, ginseng, polygonatum sibiricum and angelica sinensis;

[0079] The inlet air temperature of the spray drying is 130°C and the outlet air temperature is 80°C;

[0080] (4) Ethanol extraction

[0081] Mulberry, black sesame, black bean, black wolfberry, black truffle and 85% ethanol solution by volume were mixed at a solid-liquid ratio of 1:10 g / mL, and ultrasonically extracted for 30 min at a frequency of 60 kHz and a temperature of 65°C, filtered, and the filtrate was spray-dried to obtain an ethanol extraction powder;

[0082] The inlet air temperature of the spray drying is 130°C and the outlet air temperature is 80°C;

[0083] (5) Water extraction

[0084] Mix Cistanche deserticola, Ziziphus jujuba seeds, lily, citron and water at a solid-liquid ratio of 1:8 g / mL, heat and extract at 100°C for 40 min, filter, mix the residue at a solid-liquid ratio of 1:8 g / mL, heat and extract at 100°C for 40 min, filter, combine the filtrate, and concentrate at 75°C to 1 / 3 of the volume of the filtrate to obtain an extract concentrate;

[0085] (6) Mixing

[0086] The extract concentrate, colloidal solution, animal source powder, fermentation powder, ethanol extract powder, γ-aminobutyric acid and isomaltooligosaccharide are mixed and heated with stirring at 100° C. until the mixture is thickened to obtain the Kidney Essence Strengthening Ointment.

[0087] Comparative Example 1

[0088] The specific implementation is the same as that of Example 1, except that the composite enzyme is composed of trypsin, serrapeptase and papain in a weight ratio of 2:1:1, the enzyme activity of trypsin is 250,000 U / g, the enzyme activity of serrapeptase is 150,000 U / g, and the enzyme activity of bromelain is 100,000 U / g; the addition amount of the composite enzyme is 2% of the total mass of the seahorse, deer whip and oyster; the ultrasonic enzymolysis is carried out for 3 hours at a frequency of 30 kHz, a temperature of 50°C and a pH of 7.

[0089] Comparative Example 2

[0090] The specific implementation is the same as that of Example 1, except that the composite bacterial agent is composed of saccharomyces cerevisiae, Lactobacillus plantarum and Bifidobacterium breve in a weight ratio of 2:2:2; saccharomyces cerevisiae CICC ® 1470, from China Industrial Microbiological Culture Collection Management Center, with a bacterial activity of 20 billion cfu / g; Lactobacillus plantarum was purchased from Tianben Biotechnology (Shanxi) Co., Ltd., item number tb-0625-069, with a bacterial activity of 10 billion cfu / g; Bifidobacterium breve was purchased from Shanxi Bohong Biotechnology Co., Ltd., item number BHSW-1029-450, with a bacterial activity of 10 billion cfu / g; the inoculation amount of the composite bacterial agent was 5% of the total mass of Rehmannia glutinosa, Eucommia ulmoides male pollen, cinnamon bark, ginseng, Polygonatum sibiricum, and Angelica sinensis; the composite bacterial agent was anaerobically fermented at 37°C and pH 7 for 4 days.

[0091] Comparative Example 3

[0092] The specific implementation is the same as that of Example 1, except that the volume percentage of the ethanol solution in step (4) is 50%.

[0093] Test Example 1

[0094] Animal experiments

[0095] (1) Sleep

[0096] 70 3-month-old SPF Kunming mice weighing 20-25 g were selected, half of which were male and half were female, and they were evenly divided into 7 groups, with 10 mice in each group.

[0097] Example group: Each mouse was gavaged with 1.76 g / kg of Example 1-3 Shenjing Guyuan Paste according to body weight every day;

[0098] Comparative example group: each mouse was gavaged with 1.76 g / kg of comparative example 1-3 Shenjing Guyuan paste according to body weight every day;

[0099] Blank group: The mice were intragastrically administered with the same mass of normal saline.

[0100] Each group was intraperitoneally injected with 50 mg / kg sodium pentobarbital 1 hour after oral administration every day. The time from the injection of sodium pentobarbital to falling asleep and the sleeping time (the time from the beginning of falling asleep to waking up) of each group of mice were observed and recorded for 30 days, and the average value was taken. The specific results are shown in Table 1.

[0101] The sleep time was the time from injection to the disappearance of righting reflex; the sleep time was the time from the disappearance of righting reflex to its recovery.

[0102] Table 1 Time to sleep and sleep duration in each group

[0103] Group Time to fall asleep (min) Sleep time (min) Example 1 3.87 95.58 Example 2 3.94 92.34 Example 3 3.72 93.63 Comparative Example 1 5.28 80.76 Comparative Example 2 5.93 74.52 Comparative Example 3 4.15 82.41 Blank control 10.39 50.35

[0104] It can be seen from the data in Table 1 that Examples 1-3 can significantly shorten the time to fall asleep and prolong the sleep time compared to the blank control group. It can be seen from the data in Example 1 and Comparative Examples 1-3 that the use of different complex enzymes, complex enzymes and ethanol solution concentrations has a certain effect on the sleep quality of the Kidney Essence Guyuan Paste. The Kidney Essence Guyuan Paste prepared by the present invention can significantly improve the sleep quality.

[0105] (2) Sexual function

[0106] 80 3-month-old SPF-grade Kunming male mice weighing 20-25 g were selected and randomly divided into 8 groups, with 10 mice in each group.

[0107] 7 groups of them were modeled according to the method of 1.1 animal modeling in Example 2 of CN118638687A invention patent to 70 SPF grade Kunming male mice: 0.1ml / 10g intraperitoneal injection of 0.4mg / mL estradiol benzoate soybean oil diluent, once a day. Continuous injection for 15 days, resulting in kidney yang deficiency model. Modeling success criteria: model mice sweat and erect fur, listlessness, arched back atrophy, like to squeeze and lie but not like to move. Anal temperature dropped significantly, swimming time decreased, mouse grip weakened, autonomous activity number decreased, serum creatinine and urea increased, red blood cell parameters increased, white blood cell parameters decreased or unchanged, testis and seminal vesicle index decreased. It was observed that 7 groups of mice were successfully modeled. Another group was not modeled as a blank control.

[0108] Example group: Each mouse was gavaged with 1.76 g / kg of Example 1-3 Shenjing Guyuan Paste according to body weight every day;

[0109] Comparative example group: each mouse was gavaged with 1.76 g / kg of comparative example 1-3 Shenjing Guyuan paste according to body weight every day;

[0110] Model group: The mice were intragastrically administered with the same amount of saline.

[0111] Blank control group: gavage with the same mass of normal saline.

[0112] After continuous administration for 15 days, the mice were killed, and the organ weights of the preputial gland, testis, prostate-spermatocyst gland were weighed to calculate the reproductive organ coefficient, which is organ weight / body weight. The left epididymis of the mice was separated, placed in 20 mL PBS solution, cut into pieces, and repeatedly blown to free the sperm. After incubation at 37°C for 30 minutes, the number of sperm was counted using a cell counter. The specific results are shown in Table 2.

[0113] Table 2 Effects of each group on sexual function of mice

[0114] Group Preputial gland mg / g Testis mg / g Prostate-seminal vesicle mg / g <![CDATA[Sperm count (×10 6 )]]> Example 1 1.37 7.53 5.32 14.93 Example 2 1.32 7.47 5.20 14.42 Example 3 1.35 7.50 5.25 14.68 Comparative Example 1 1.13 6.32 4.46 10.94 Comparative Example 2 1.07 6.03 4.28 10.25 Comparative Example 3 1.21 6.94 4.84 12.31 Model Group 0.58 4.53 2.43 7.28 Blank control 1.82 8.56 5.95 16.42

[0115] As can be seen from the data in Table 2, Examples 1-3 can significantly improve the organ indexes of the preputial gland, testicle, prostate-spermatic vesicle relative to the model group. As can be seen from the data in Example 1 and Comparative Examples 1-3, the use of different complex enzymes, complex enzymes, and ethanol solution concentrations have a certain effect on the improvement of male function by the Kidney Essence Guyuan Paste. The Kidney Essence Guyuan Paste prepared by the present invention can protect the reproductive system and has a yang-enhancing effect.

[0116] Test Example 2

[0117] Clinical Research

[0118] The efficacy of Kidney Essence Guyuan Paste was verified by taking insomnia caused by deficiency of both heart and spleen as an example.

[0119] (1) Diagnostic criteria

[0120] TCM diagnostic criteria: formulated in accordance with TCM Disease Diagnosis and Efficacy Criteria, in line with TCM diagnostic criteria for insomnia due to deficiency of both the heart and spleen, with symptoms such as frequent dreams, easy awakening, haziness, forgetfulness, palpitations, fatigue, pale tongue, and weak pulse.

[0121] Western medicine diagnostic criteria: Based on the "Guidelines for the Diagnosis and Treatment of Insomnia in China", with sleep disorders as the main manifestation, accompanied by symptoms such as shallow sleep, difficulty falling asleep, early awakening, and easy awakening; sleep disorders occur ≥3 times a week and last for >1 month; non-mental disorder symptoms or physical illness.

[0122] Inclusion criteria: those who had not used hypnotic drugs in the past 6 months; those who gave informed consent to the study; and those whose Pittsburgh Sleep Quality Index (PSQI) score was ≥ 7 points.

[0123] Exclusion criteria: those who have used sleeping pills within 6 months; pregnant or lactating women; those with severe heart, brain, kidney, liver and blood system diseases; those with anxiety, depression, schizophrenia and other mental illnesses.

[0124] A total of 80 patients with insomnia due to deficiency of both heart and spleen, who met the diagnostic criteria of traditional Chinese and Western medicine and the inclusion criteria, were selected and randomly divided into two groups. There were no significant differences in age, gender, and PSQI scores between the two groups.

[0125] (2) Treatment methods

[0126] Control group: Drink Tong Ren Tang Ziziphus jujuba, lily and Poria tea one hour before bedtime. Put one bag into 150-200mL boiling water for 3-5 minutes before drinking.

[0127] Example group: Take 10 g of the Shenjing Guyuan Paste prepared in Example 1 on an empty stomach every morning, once a day.

[0128] (3) Efficacy observation criteria

[0129] The therapeutic effect was determined based on the PSQI score.

[0130] Recovery: PSQI score reduction rate ≥ 90%, sleep time > 6h, and full of energy the next day;

[0131] Significantly effective: PSQI score reduction rate 60%-89%, sleep time increased ≥3h, sleep significantly improved;

[0132] Effective: PSQI score reduction rate is 30%-59%, sleep time increases by <3h, and sleep improves;

[0133] Invalid: Failed to meet the above criteria.

[0134] Total effective rate = (number of cured cases + markedly effective cases + effective cases) / 40×100%.

[0135] (4) Clinical efficacy

[0136] After taking the medicine for one month, the clinical efficacy of the control group and the embodiment group was determined according to the efficacy observation standard. The specific results are shown in Table 3.

[0137] Table 3 Clinical efficacy of the control group and the embodiment group

[0138] Group Recovered (case) Significantly effective (example) Valid (Example) Invalid (Example) Total effective rate (%) Control group 18 12 7 3 92.5 Example Group 10 15 7 8 80

[0139] As shown in Table 3, the Kidney Essence Strengthening Ointment of the present invention has an excellent therapeutic effect on treating insomnia of deficiency of both heart and spleen.

[0140] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A ginseng essence and essence paste containing multiple medicinal materials, characterized in that: The invention comprises the following raw materials in parts by weight: 8-12 parts of seahorse, 8-12 parts of deer whip, 8-12 parts of Cistanche deserticola, 8-12 parts of Rehmannia root, 8-12 parts of mulberry, 8-12 parts of black sesame, 8-12 parts of black bean, 8-12 parts of black wolfberry, 3-7 parts of black truffle, 8-12 parts of oyster, 3-7 parts of Eucommia male pollen, 8-12 parts of cinnamon, 8-12 parts of ginseng, 8-12 parts of polygonatum, 3-7 parts of donkey-hide gelatin, 8-12 parts of spiny jujube kernel, 8-12 parts of lily, 8-12 parts of citron and 8-12 parts of angelica.

2. The ginseng essence and essence paste according to claim 1, characterized in that Also includes gamma-aminobutyric acid and isomaltooligosaccharides.

3. The method for preparing the Kidney Essence Strengthening Ointment according to claim 1 or 2, characterized in that: include: All raw materials are dried and crushed, seahorse, deer whip, oyster and water are mixed and heated, composite enzyme is added for ultrasonic enzymolysis, filtered, and the enzymolysis solution is spray-dried to obtain animal-based powder; Rehmannia root, Eucommia male pollen, cinnamon, ginseng, polygonatum, and angelica are mixed with water, inoculated with composite bacterial agent for fermentation, filtered, and the fermentation solution is spray-dried to obtain fermentation powder; mulberry, black sesame, black bean, black wolfberry, and black truffle are mixed with ethanol solution, ultrasonically extracted, filtered, and the extract is spray-dried to obtain ethanol extraction powder; Cistanche, Ziziphus jujuba, lily, and citron are mixed with water, heated for extraction, filtered, and concentrated to obtain an extraction concentrate; the extraction concentrate, donkey-hide gelatin, animal-based powder, fermentation powder, and ethanol extraction powder are mixed, heated, and stirred until the flag is hung to obtain the kidney essence consolidating paste.

4. The preparation method according to claim 3, characterized in that: The particle size of the crushed particles is 80-500 meshes.

5. The preparation method according to claim 3, characterized in that: The composite enzyme is composed of flavor protease, snail protease and bromelain in a weight ratio of (1-3): 1:1; the added amount of the composite enzyme is 1%-3% of the total mass of the seahorse, deer whip and oyster; the frequency of the ultrasonic enzymolysis is 20-40kHz, the temperature is 40-50°C, the pH value is 6.5-7.5, and the time is 2-5h.

6. The preparation method according to claim 3, characterized in that: The composite bacterial agent is composed of Saccharomyces boulardii, Lactobacillus rhamnosus and Bifidobacterium longum subspecies longum in a weight ratio of (1-3):(1-3):(1-3); the inoculation amount of the composite bacterial agent is 3%-8% of the total mass of the Rehmannia root, Eucommia ulmoides male pollen, cinnamon bark, ginseng, Polygonatum sibiricum and Angelica sinensis, the temperature is 35-40°C, the pH value is 6.5-7.5, and the time is 2-5d.

7. The preparation method according to claim 3, characterized in that: The volume percentage of the ethanol solution is 75%-85%; the material-liquid ratio of the ultrasonic extraction is 1:5-10g / mL, the frequency is 40-60kHz, the temperature is 55-65°C, the number of times is 1-3 times, and each time is 20-30min.

8. The preparation method according to claim 3, characterized in that: The solid-liquid ratio of the heating extraction is 1:4-8 g / mL, the temperature is 90-100° C., the number of times is 2-4 times, and each time is 30-40 minutes.

9. The preparation method according to claim 3, characterized in that: The temperature of the heating and stirring is 90-100°C.

10. Use of the ginseng essence and solidifying essence paste according to claim 1 or 2 in preparing a medicine for alleviating symptoms of deficiency of both heart and spleen.