Plant extract for treating breeder cock cloaca inflammation as well as preparation method and application thereof

Abstract

The invention discloses a plant extract for treating cloaca inflammation of breeder cocks. The plant extract is prepared from the following raw materials in parts by weight: 45-55 parts of luffa leaves, 30-40 parts of pumpkin peels, 25-30 parts of selfheal, 25-30 parts of hairyvein agrimony, 20-25 parts of lalang grass rhizome, 15-20 parts of mulberry leaves and 3-5 parts of peanut coats. The invention also discloses a preparation method and application of the plant extract for treating cloaca inflammation of breeder cocks. Various natural plant raw materials are adopted, the source is wide, and the cost is relatively low; the plant raw materials have a plurality of bioactive components which have a synergistic effect, can effectively treat cloaca inflammation of breeder cocks, and are safer and free of residues compared with traditional antibiotic drugs; the pollution to the environment can be reduced, and the requirement of sustainable development is met.

Description

Technical Field

[0001] The invention belongs to the technical field of poultry breeding, and particularly relates to a plant extract for treating cloacal inflammation in breeding roosters, and a preparation method and application thereof. Background Art

[0002] Poultry farming is an important part of my country's animal husbandry and a pillar industry for rural economic development. With the continuous improvement of artificial insemination technology, artificial insemination has basically replaced traditional natural breeding, greatly reducing the number of roosters raised, increasing the fertilization rate of breeding eggs, and accelerating the process of poultry breeding.

[0003] During artificial insemination, improper operation by the semen collector, such as rough movements or excessive force due to unskilled techniques, as well as infection with pathogens such as Salmonella and E. coli, can cause damage to the cloaca of the breeder rooster. Mixed insemination is often used in breeder production, and if the cloaca of a rooster is damaged, all the semen in the same semen collection cup will be contaminated and invalidated. Semen from more than a dozen roosters is usually collected in one semen collection cup. If contaminated, it will affect the normal insemination of 300 to 400 hens.

[0004] When the cloaca of breeding roosters is damaged, traditional treatment methods are rather tricky: for example, the model of administering medicine through drinking water, because current poultry farming is intensive and large-scale, chickens that do not need medicine will also be fed, resulting in increased drug use costs; if antibiotics are fed, residual drugs will appear in the breeding eggs, resulting in an increased disability rate of hatched chicks; the model of administering medicine mixed with feed, because the digestive tract of chickens is very short, the food they eat will be emptied quickly, so the treatment effect is not ideal; if a normal rooster is used to replace a rooster with a damaged cloaca, it is easy to cause a stress response in the new individual, and it will take more than ten days or even longer to adjust before semen can be collected normally.

[0005] Therefore, there is an urgent need for a green, environmentally friendly, and easy-to-handle plant extract for treating cloacal inflammation in roosters. Summary of the invention

[0006] An object of the present invention is to solve at least the above-mentioned drawbacks and to provide advantages which will be described hereinafter.

[0007] Another object of the present invention is to provide a plant extract capable of treating cloacal inflammation in breeder roosters with stable effects.

[0008] Another object of the present invention is to provide a plant extract that can effectively solve the problem of cloacal inflammation in breeding roosters.

[0009] In order to achieve these purposes and other advantages of the present invention, a plant extract for treating cloacal inflammation in breeding roosters is provided, comprising the following raw materials in parts by weight: 45-55 parts of loofah leaves, 30-40 parts of pumpkin peels, 25-30 parts of selfheal, 25-30 parts of agrimony, 20-25 parts of Imperata roots, 15-20 parts of mulberry leaves, and 3-5 parts of peanut skins.

[0010] In the above scheme, specifically, loofah leaves have the effects of clearing away heat and detoxifying, stopping bleeding, and removing summer heat. The various active ingredients contained in them can effectively inhibit inflammatory reactions, promote wound healing, and have a positive effect on the treatment of cloacal inflammation in breeding roosters. They play a major therapeutic role in the formula and serve as the main medicine; pumpkin peel is rich in nutrients such as vitamins, minerals and dietary fiber, and has the functions of anti-inflammatory, antibacterial and enhancing immunity, which helps to relieve the inflammatory symptoms of the cloaca of breeding roosters and improve the body's resistance. At the same time, it can alleviate the cold nature of the main medicine and play a supporting role as an adjuvant; Prunella vulgaris can clear the liver and purge fire, improve eyesight, disperse nodules and reduce swelling. Its active ingredients help to eliminate inflammation and reduce the redness and swelling symptoms of the cloaca of breeding roosters. As a ministerial medicine, it has a synergistic therapeutic effect; Herba Agrimoniae has the effects of astringent hemostasis, dysentery and insecticide, which can not only It can relieve the symptoms of cloacal bleeding, inhibit the growth of pathogens, prevent the infection from getting worse, and assist the main medicine in playing a therapeutic role as a ministerial medicine; Imperata root can cool blood and stop bleeding, clear away heat and promote diuresis, promote the excretion of toxins in the body, and reduce the impact of inflammation on the body of breeding roosters. At the same time, it has a certain alleviating effect on bleeding symptoms, and assists the main medicine in clearing damp heat in the body as an adjuvant; mulberry leaves have the effects of dispersing wind-heat, clearing the lungs and moisturizing dryness, clearing the liver and improving eyesight. The flavonoids and other ingredients it contains have anti-inflammatory and antioxidant effects, which help to improve the inflammatory state of the cloaca of breeding roosters and play an adjuvant role in auxiliary treatment; peanut skins have the effects of stopping bleeding, dispersing blood stasis, and reducing swelling, which can effectively relieve the bleeding symptoms of the cloaca of breeding roosters, promote wound healing, and can guide various medicines to the diseased area. At the same time, it harmonizes various medicines and makes the efficacy of the entire formula more coordinated and unified.

[0011] First of all, by adopting a variety of natural plant raw materials, the sources are wide and the cost is relatively low.

[0012] Secondly, these plant raw materials contain multiple biologically active ingredients that work synergistically with each other and can effectively treat cloacal inflammation in breeding roosters. Compared with traditional antibiotics, they are safer and leave no residue, and will not cause adverse effects on the health and reproductive system of breeding roosters, nor will they leave harmful substances in poultry products, thus ensuring food safety.

[0013] In addition, the use of natural plant raw materials can reduce pollution to the environment and meet the requirements of sustainable development.

[0014] Preferably, the plant extract for treating cloacal inflammation in breeding roosters further comprises the following raw materials in parts by weight: 30 to 35 parts of Patrinia scabra.

[0015] In the above scheme, Patrinia scabra has the effects of clearing away heat and detoxifying, eliminating carbuncle and draining pus, removing blood stasis and relieving pain. It has an inhibitory effect on many pathogens, can relieve the pain and swelling caused by cloacal inflammation, and works together with loofah leaves to exert the therapeutic effects of all the medicines, and is regarded as the main medicine.

[0016] Preferably, the plant extract for treating cloacal inflammation in breeding roosters further comprises the following raw materials in parts by weight: 10 to 15 parts of pine needles.

[0017] In the above scheme, pine needles are rich in various bioactive substances, have antibacterial, antiviral, anti-inflammatory and other effects, can enhance the immunity of breeding roosters, promote the recovery of cloacal inflammation, and can assist the main medicine to play a certain therapeutic role in inflammation as an adjuvant.

[0018] A method for preparing a plant extract for treating cloacal inflammation in breeding roosters, comprising: The raw materials are obtained according to the following weight portions: 45-55 parts of loofah leaves, 30-40 parts of pumpkin peels, 30-35 parts of Patrinia scabra, 25-30 parts of Prunella vulgaris, 25-30 parts of Agrimoniae, 20-25 parts of Imperata rhizome, 15-20 parts of mulberry leaves, 10-15 parts of pine needles and 3-5 parts of peanut skins.

[0019] After mixing all the ingredients, add boiling water and stir until it becomes a paste to get a paste.

[0020] The paste is subjected to freeze-thaw treatment to obtain a freeze-thaw mixture.

[0021] The freeze-thaw mixture was mixed with 95% ethanol solution at a mass ratio of 1:3, and subjected to organic extraction and concentration treatment at room temperature for 3 hours to obtain a clear paste with a relative density of 1.18-1.25.

[0022] The clear paste and taurine are mixed in a mass ratio of 1:8.3 to obtain the plant extract for treating cloacal inflammation in breeding roosters.

[0023] In the above scheme, specifically, the raw materials are sorted, cleaned, dried and crushed before being prepared into a paste, and the raw materials are crushed to a particle size of 50 mesh. Except for the freezing step, the environment of other working steps is a temperature of 18~30°C and a relative humidity of 30%~70%. The preparation method of the present invention is simple to operate and does not require complex equipment and high cost investment. Through specific steps and conditions, the effective ingredients in the plant raw materials can be fully extracted to ensure the quality and stability of the extract. The rational use of freeze-thaw treatment, organic extraction and concentration steps improves the purity and activity of the extract, so that the final plant extract has a better therapeutic effect.

[0024] Preferably, the organic extraction specifically comprises: mixing the freeze-thaw mixture with a 95% ethanol solution in a volume ratio of 1:3, extracting at room temperature for 3 hours, repeating the filtration at least 3 times, combining the filtrate and removing the ethanol by vacuum distillation; the concentration treatment specifically comprises: placing the organically extracted extract in a vacuum rotary apparatus and concentrating it in a water bath at a constant temperature of 60°C until the relative density reaches 1.18~1.25.

[0025] In the above scheme, the specific operation details of organic extraction and concentration treatment are clarified, making the preparation process more standardized and scientific, which is conducive to improving the repeatability and stability of the preparation process. Preparation according to this specific operation method can ensure the consistency of the quality and performance of the plant extract obtained each time, which is convenient for large-scale production and application.

[0026] The invention discloses a plant extract for treating cloacal inflammation in breeder roosters, and the plant extract is used in a preparation for preventing and treating cloacal inflammation in breeder roosters.

[0027] Preferably, the cloacal inflammation of the breeder rooster is characterized by damage to the exterior of the cloaca and bleeding or purulent secretions; or no damage to the exterior of the cloaca but bleeding or purulent secretions.

[0028] Preferably, the preparation is a spray preparation to be sprayed on the lesion site outside the cloaca or a spray preparation to be injected into the lesion site of the cloaca.

[0029] Preferably, for cloacal inflammation in breeder roosters with damage to the outside of the cloaca and bleeding or purulent secretions, spray treatment is performed on the location of the damage, bleeding or purulent secretions, once a day, with a spray volume of 0.5 to 1 ml each time, for 3 to 5 consecutive days.

[0030] In the above scheme, for breeding roosters with external cloaca damage and bleeding or purulent secretions, a spray treatment method is used, which can directly act on the lesion site, increase the local concentration of the drug, effectively control inflammation, and promote wound healing.

[0031] Preferably, for cloacal inflammation in breeder roosters with no damage to the outside of the cloaca but bleeding or purulent secretions, a flat dosing nozzle is inserted into the cloaca of the breeder rooster for spraying treatment, spraying once a day, with a spray volume of 0.3 to 0.5 ml each time, for 3 to 5 consecutive days.

[0032] In the above scheme, for roosters whose cloaca is not damaged on the outside but has bleeding or purulent secretions, the nozzle is inserted into the cloaca for spraying treatment, which can make the drug act directly inside the lesion, thereby improving the pertinence and effectiveness of the treatment.

[0033] Advantages of the present invention: Firstly, the plant extract for treating cloacal inflammation in roosters of the present invention adopts a variety of natural plant raw materials, has a wide range of sources and a relatively low cost.

[0034] Secondly, the raw materials of the plant extract for treating cloacal inflammation in breeding roosters of the present invention contain a variety of biologically active ingredients, which act synergistically with each other and can effectively treat cloacal inflammation in breeding roosters. Compared with traditional antibiotics, it is safer and has no residue, and will not cause adverse effects on the health and reproductive system of breeding roosters, nor will it leave harmful substances in poultry products, thereby ensuring food safety.

[0035] In addition, the plant extract for treating cloacal inflammation in breeding roosters of the present invention adopts natural plant raw materials, which can also reduce pollution to the environment and meet the requirements of sustainable development.

[0036] In addition, the preparation method of the present invention is simple to operate and does not require complex equipment and high cost investment. Through specific steps and conditions, the effective ingredients in the plant raw materials can be fully extracted to ensure the quality and stability of the extract. The reasonable use of freeze-thaw treatment, organic extraction and concentration steps improves the purity and activity of the extract, so that the final plant extract has a better therapeutic effect.

[0037] In addition, the plant extract for treating cloacal inflammation in breeder roosters of the present invention treats the external damage of the cloaca, so that the drug can directly act on the inside of the lesion, thereby improving the pertinence and effectiveness of the treatment. DETAILED DESCRIPTION

[0038] The present invention is further described in detail below in conjunction with embodiments so that those skilled in the art can implement the invention with reference to the description.

[0039] It should be understood that the terms such as “having”, “including” and “comprising” used herein do not exclude the existence or addition of one or more other elements or combinations thereof.

[0040] It should be noted that the experimental methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials can be obtained from commercial channels unless otherwise specified.

[0041] In the description of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", and "set" should be understood in a broad sense. For example, it can be fixedly connected or set, or it can be detachably connected or set, or connected or set in one piece. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. The terms "lateral", "longitudinal", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc. indicate directions or positional relationships, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as limiting the present invention.

[0042] The plant extract for treating cloacal inflammation in breeding roosters comprises the following raw materials in parts by weight: 45-55 parts of loofah leaves, 30-40 parts of pumpkin peels, 25-30 parts of selfheal, 25-30 parts of agrimony, 20-25 parts of Imperata roots, 15-20 parts of mulberry leaves and 3-5 parts of peanut skins.

[0043] Furthermore, the plant extract for treating cloacal inflammation in breeding roosters also includes the following raw materials in parts by weight: 30 to 35 parts of Patrinia scabra.

[0044] Furthermore, the plant extract for treating cloacal inflammation in breeding roosters also includes the following raw materials in parts by weight: 10 to 15 parts of pine needles.

[0045] The invention discloses a plant extract for treating cloacal inflammation in breeder roosters, and the plant extract is used in a preparation for preventing and treating cloacal inflammation in breeder roosters.

[0046] Furthermore, the cloacal inflammation of the breeder rooster is characterized by damage to the exterior of the cloaca and bleeding or purulent secretions; or no damage to the exterior of the cloaca but bleeding or purulent secretions.

[0047] Furthermore, the preparation is a spray preparation that is sprayed on the lesion site outside the cloaca or a spray preparation that is injected into the lesion site of the cloaca.

[0048] Furthermore, for cloacal inflammation in breeder roosters with damage to the outside of the cloaca and bleeding or purulent secretions, spray the damaged, bleeding or purulent secretions once a day with a spray volume of 0.5 to 1 ml each time for 3 to 5 consecutive days.

[0049] Furthermore, for cloacal inflammation in breeder roosters with no damage to the outside of the cloaca but bleeding or purulent secretions, insert a flat dosing nozzle into the cloaca of the breeder rooster for spraying treatment, spray once a day, with a spray volume of 0.3~0.5 ml each time, and spray continuously for 3~5 days.

[0050] Example 1 45 parts of loofah leaves, 30 parts of pumpkin peels, 25 parts of selfheal, 25 parts of agrimony, 20 parts of Imperata root, 15 parts of mulberry leaves and 5 parts of peanut skins are taken respectively, and the mixtures are sorted, washed, dried and crushed in sequence; after mixing, boiling water is added and stirred until the mixture becomes a paste to obtain a paste; the paste is transferred to -25°C and frozen for at least 24 hours to obtain a frozen block; the frozen block is placed at 25°C for natural thawing to obtain a freeze-thaw mixture; the freeze-thaw mixture is mixed with a 95% ethanol solution at a mass ratio of 1:3, extracted at room temperature for 3 hours, and the filtration is repeated at least 3 times, the filtrate is combined and the ethanol is evaporated by vacuum distillation; the mixture is then placed in a vacuum rotary instrument and concentrated in a water bath at a constant temperature of 60°C to a relative density of 1.18-1.25 to obtain a clear paste; the clear paste is mixed with taurine at a mass ratio of 1:8.3 to obtain the plant extract for treating cloacal inflammation in breeding roosters.

[0051] The raw material was crushed to a particle size of 50 mesh.

[0052] Example 2 45 parts of loofah leaves, 30 parts of pumpkin peels, 25 parts of selfheal, 25 parts of agrimony, 20 parts of Imperata root, 15 parts of mulberry leaves, 5 parts of peanut skins and 30 parts of Patrinia chinensis are taken respectively, and the mixtures are sorted, washed, dried and crushed in sequence; after mixing, boiling water is added and stirred until the mixture becomes a paste to obtain a paste; the paste is transferred to -25°C and frozen for at least 24 hours to obtain a frozen block; the frozen block is placed at 25°C for natural thawing to obtain a freeze-thaw mixture; the freeze-thaw mixture is mixed with a 95% ethanol solution at a mass ratio of 1:3, extracted at room temperature for 3 hours, and the filtration is repeated at least 3 times, the filtrate is combined and the ethanol is evaporated by vacuum distillation; the mixture is then placed in a vacuum rotary instrument and concentrated in a water bath at a constant temperature of 60°C to a relative density of 1.18-1.25 to obtain a clear paste; the clear paste is mixed with taurine at a mass ratio of 1:8.3 to obtain the plant extract for treating cloacal inflammation in breeding roosters.

[0053] The raw material was crushed to a particle size of 50 mesh.

[0054] Example 3 45 parts of loofah leaves, 30 parts of pumpkin peels, 25 parts of selfheal, 25 parts of agrimony, 20 parts of Imperata root, 15 parts of mulberry leaves, 5 parts of peanut skins, 30 parts of Patrinia and 10 parts of pine needles are taken respectively, and the mixture is sorted, washed, dried and crushed in sequence; after mixing, boiling water is added and stirred until it becomes a paste to obtain a paste; the paste is transferred to -25°C and frozen for at least 24 hours to obtain a frozen block; the frozen block is placed at 25°C for natural thawing to obtain a freeze-thaw mixture; the freeze-thaw mixture is mixed with a 95% ethanol solution in a mass ratio of 1:3, extracted at room temperature for 3 hours, and the suction filtration is repeated at least 3 times, the filtrate is combined and the ethanol is evaporated by reduced pressure distillation; the filtrate is placed in a vacuum rotary instrument and concentrated in a water bath at a constant temperature of 60°C to a relative density of 1.18-1.25 to obtain a clear paste; the clear paste is mixed with taurine in a mass ratio of 1:8.3 to obtain the plant extract for treating cloacal inflammation in breeding roosters.

[0055] The raw material was crushed to a particle size of 50 mesh.

[0056] Comparative Example 1 35 parts of Patrinia scabra, 30 parts of pumpkin peel, 25 parts of Prunella vulgaris, 25 parts of Agrimony scoparia, 20 parts of Imperata root, 15 parts of mulberry leaf, 10 parts of pine needle and 5 parts of peanut skin are taken respectively, and they are sorted, washed, dried and crushed in sequence; after mixing, boiling water is added and stirred until it becomes a paste to obtain a paste; the paste is transferred to -25°C and frozen for at least 24 hours to obtain a frozen block; the frozen block is placed at 25°C for natural thawing to obtain a freeze-thaw mixture; the freeze-thaw mixture is mixed with a 95% ethanol solution in a mass ratio of 1:3, extracted at room temperature for 3 hours, and the suction filtration is repeated at least 3 times, the filtrate is combined and the ethanol is evaporated by reduced pressure distillation; the filtrate is then placed in a vacuum rotary instrument and concentrated in a water bath at a constant temperature of 60°C to a relative density of 1.18-1.25 to obtain a clear paste; the clear paste is mixed with taurine in a mass ratio of 1:8.3 to obtain the plant extract for treating cloacal inflammation in breeding roosters.

[0057] The raw material was crushed to a particle size of 50 mesh.

[0058] Comparative Example 2 200 portions of loofah leaves are selected, cleaned, dried and crushed in sequence; after mixing, boiling water is added and stirred until it becomes a paste to obtain a paste; the paste is transferred to -25°C and frozen for at least 24 hours to obtain a frozen block; the frozen block is naturally thawed at 25°C to obtain a freeze-thaw mixture; the freeze-thaw mixture is mixed with a 95% ethanol solution in a mass ratio of 1:3, extracted at room temperature for 3 hours, and the filtration is repeated at least 3 times, the filtrate is combined and the ethanol is evaporated by reduced pressure distillation; the filtrate is then placed in a vacuum rotary instrument and concentrated in a water bath at a constant temperature of 60°C to a relative density of 1.18-1.25 to obtain a clear paste; the clear paste is mixed with taurine in a mass ratio of 1:8.3 to obtain the plant extract for treating cloacal inflammation in breeding roosters.

[0059] The raw material was crushed to a particle size of 50 mesh.

[0060] Test and analysis Experiment 1: The experiment was conducted at a poultry research and breeding base of a vocational college in Taizhou City, Jiangsu Province. The subjects were 642 adult breeder roosters that were eliminated because their feather color, comb shape, and claw color did not meet the breeding and selection requirements. Before the experiment, students majoring in animal husbandry at the school practiced artificial semen collection on the eliminated adult breeder roosters. These students had never practiced artificial semen collection techniques before. When learning artificial semen collection this time, their movements were inevitably irregular, and the strength of semen collection was not accurately controlled, which inevitably caused artificial damage to the cloaca of the breeder roosters. After the exercise, the number of roosters with damaged cloaca was counted, a total of 112, and the roosters with damaged cloaca were randomly divided into 7 groups, and the skilled breeders treated the roosters with damaged cloaca in this batch, wherein Groups 1 to 3 were treated with the plant extracts for treating cloacal inflammation of roosters prepared in Examples 1 to 3; Groups 4 to 6 were treated with the plant extracts for treating cloacal inflammation of roosters prepared in Examples 1 to 3 according to the damage of the cloaca by spraying or injecting; Group 7 was treated by applying iodine to the cloaca; the treatment was continued for 5 days, and semen collection was stopped during the treatment period. On the 6th day, the skilled breeders collected semen from this batch of roosters, and the cloaca was not red and swollen, and normal semen could be collected, which was considered to be restored to normal. The results are shown in Table 1. The spray treatment is specifically as follows: for breeder roosters with external cloaca damage and bleeding or purulent secretions, spray the damaged, bleeding or purulent secretions once a day with a spray volume of 0.5 to 1 ml each time; for breeder roosters with no external cloaca damage but bleeding or purulent secretions, insert the dosing flat nozzle into the breeder rooster's cloaca for spray treatment, spray once a day with a spray volume of 0.3 to 0.5 ml each time.

[0061] Table 1: The situation of each group of breeder roosters project Initial number of pieces Cumulative number of patients who improved on the first day (patients) Cumulative number of patients who improved on the second day (patients) Cumulative number of improvement on the 3rd day () Cumulative number of improvement on the 4th day () Cumulative number of improvement on the 5th day () Return to normal quantity (pieces) Group 1 16 2 6 10 14 16 13 Group 2 16 4 9 12 16 / 14 Group 3 16 5 8 14 16 / 15 Group 4 16 5 9 13 16 / 14 Group 5 16 6 9 15 16 / 15 Group 6 16 6 10 15 16 / 15 Group 7 16 2 4 7 10 11 7 According to the data in Table 1, the plant extract for treating cloacal inflammation in roosters of the present invention not only has a faster effect on roosters with damaged cloaca but also has a better recovery effect on roosters.

[0062] Experiment 2: The experiment was conducted at the Guangxi Local Chicken Teaching and Breeding Base of a certain Animal Science and Technology College in Nanning, Guangxi. The subjects were 420 adult breeder roosters that were eliminated because their feather color, comb shape, and claw color did not meet the breeding and selection requirements. Before the experiment, students majoring in animal husbandry at the school practiced artificial semen collection on the eliminated adult breeder roosters. These students had never practiced artificial semen collection techniques before. When learning artificial semen collection this time, their movements were inevitably irregular, and the strength of semen collection was not accurately controlled, which inevitably caused artificial damage to the cloaca of the breeder roosters. After the practice, the number of breeder roosters with damaged cloaca was counted, totaling 80. The roosters with damaged cloaca were randomly divided into 7 groups, and the roosters with damaged cloaca were treated by skilled breeders. Group 1 was treated with the plant extract for treating cloacal inflammation of roosters prepared in Example 1; Groups 2 to 4 were treated with the plant extract for treating cloacal inflammation of roosters prepared in Examples 1 to 3, respectively, for spraying or injection according to the condition of cloaca damage; Group 5 was treated by applying iodine to the cloaca; the treatment was continued for 5 days, and semen collection was stopped during the treatment period. On the 6th day, the breeders were treated with semen collection. When there was no redness or swelling in the cloaca and normal semen could be collected, it was considered to have recovered to normal. The results are shown in Table 2. The spray treatment is specifically as follows: for breeder roosters with external cloaca damage and bleeding or purulent secretions, spray the damaged, bleeding or purulent secretions once a day with a spray volume of 0.5 to 1 ml each time; for breeder roosters with no external cloaca damage but bleeding or purulent secretions, insert the dosing flat nozzle into the breeder rooster's cloaca for spray treatment, spray once a day with a spray volume of 0.3 to 0.5 ml each time.

[0063] Table 2: The situation of each group of breeder roosters project Initial number of pieces Cumulative number of patients who improved on the first day (patients) Cumulative number of patients who improved on the second day (patients) Cumulative number of improvement on the 3rd day () Cumulative number of improvement on the 4th day () Cumulative number of improvement on the 5th day () Return to normal quantity (pieces) Group 1 16 3 5 9 12 16 12 Group 2 16 5 6 11 15 16 14 Group 3 16 6 8 11 16 / 14 Group 4 16 6 8 12 15 16 15 Group 5 16 2 3 7 11 13 7 Among them, 2 animals in Group 5 died during the experiment. After autopsy, it was found that the cause of death was severe damage to the cloaca, which caused the wound to be contaminated by feces and other complications.

[0064] According to the data in Table 2 and the test records, the plant extract for treating cloacal inflammation in breeder roosters of the present invention not only has a faster effect on breeder roosters with damaged cloaca but also has a better recovery effect on breeder roosters, and effectively avoids the problem of aggravation of cloacal damage and causing other complications.

[0065] Test 3: The test site was a breeder farm of a company in Guitou Village, Taoxu Town, Hengzhou City, Nanning City, Guangxi Province. 250 breeder roosters with damaged cloaca were randomly selected and divided into 2 groups. The plant extracts for treating cloacal inflammation in breeder roosters prepared in Examples 1 and 3 of the present invention were sprayed or injected according to the condition of cloaca damage; the control group was sprayed or injected with iodine; the comparison groups 1 and 2 were sprayed or injected with the extracts prepared in Comparative Examples 1 and 2; wherein the spray treatment was specifically as follows: for breeder roosters with damaged cloaca and bleeding or purulent secretions, spray treatment was performed on the damaged, bleeding or purulent secretions, once a day, and the amount of each spray was 0.5 to 1 ml; for breeder roosters with no damage on the outside of the cloaca but bleeding or purulent secretions, the flat spray head was inserted into the cloaca of the breeder rooster for spray treatment, once a day, and the amount of each spray was 0.3 to 0.5 ml. The test period was 7 days. The recovery of the breeding roosters was recorded on the 3rd, 5th and 7th days. At the same time, skilled breeders collected artificial semen from each group of breeding roosters on the 3rd, 5th and 7th days. The roosters were considered to have recovered to normal if there was no redness or swelling in the cloaca and normal semen could be collected. The results are shown in Table 3.

[0066] Table 3: According to the data in Table 3, most of the roosters with damaged cloaca have basically improved after being treated with the plant extract for treating cloacal inflammation in roosters of the present invention for 3 days, and the improvement rate can reach 100% after 5 days; the recovery rate can reach 94% after 6 days, and complete recovery can be achieved after 7 days, which has obvious advantages over the traditional treatment with iodine.

[0067] Compared with test group 1, test group 2 added two raw materials, Patrinia salsa and pine needles. By comparing the data of test group 1 and test group 2, it can be seen that the effect of the formula with the addition of Patrinia salsa and pine needles in treating cloacal inflammation in breeding roosters has been further improved.

[0068] Compared with the test group 1, the comparison group 1 lacks loofah leaves and has more Patrinia and pine needles. By comparing the data of the two groups, it can be seen that the effect of the test group 1 in relieving the symptoms of cloacal inflammation of the breeding roosters is obviously better, and the breeding roosters in the test group 1 reach a normal reproductive state much faster than those in the comparison group 1, indicating that according to the prescription of the present invention, the loofah leaves as the main medicine act synergistically with other components, which can more quickly promote the improvement of the symptoms of cloacal inflammation in the breeding roosters, and can play a continuous and stable role in the treatment process, and promote the breeding roosters to restore normal reproductive function.

[0069] From the data of comparison test group 1 and comparison group 2, it can be seen that the single loofah leaf extract has a slow progress in improving cloacal inflammation in breeding roosters, and its effect on restoring the reproductive function of breeding roosters with cloacal inflammation is relatively delayed.

[0070] Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and the embodiments. They can be applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily realized.

Claims

1. A plant extract for treating cloacal inflammation in roosters, characterized in that: The invention comprises the following raw materials in parts by weight: 45-55 parts of loofah leaves, 30-40 parts of pumpkin peels, 25-30 parts of selfheal, 25-30 parts of agrimony, 20-25 parts of Imperata rhizomes, 15-20 parts of mulberry leaves and 3-5 parts of peanut skins.

2. The plant extract for treating cloacal inflammation in roosters according to claim 1, characterized in that: The following raw materials are also included in parts by weight: 30-35 parts of Patrinia salsa.

3. The plant extract for treating cloacal inflammation in roosters according to claim 2, characterized in that: The following raw materials are also included in weight: 10~15 parts of pine needles.

4. The method for preparing the plant extract for treating cloacal inflammation in roosters according to claim 1, characterized in that: include: The raw materials are obtained according to the following weight parts: 45-55 parts of loofah leaves, 30-40 parts of pumpkin peels, 30-35 parts of Patrinia scabra, 25-30 parts of Prunella vulgaris, 25-30 parts of Agrimoniae, 20-25 parts of Imperata cylindrica, 15-20 parts of mulberry leaves, 10-15 parts of pine needles, and 3-5 parts of peanut skins; After mixing all the ingredients, add boiling water and stir until it becomes a paste to obtain a paste; subjecting the paste to freeze-thaw treatment to obtain a freeze-thaw mixture; The freeze-thaw mixture was mixed with 95% ethanol solution at a mass ratio of 1:3, and organic extraction and concentration were performed at room temperature for 3 hours to obtain a clear paste with a relative density of 1.18-1.25; The clear paste and taurine are mixed in a mass ratio of 1:8.3 to obtain the plant extract for treating cloacal inflammation in breeding roosters.

5. The method for preparing the plant extract for treating cloacal inflammation in roosters according to claim 4, characterized in that: The organic extraction specifically includes: mixing the freeze-thaw mixture with a 95% ethanol solution at a volume ratio of 1:3, extracting at room temperature for 3 hours, repeatedly filtering at least 3 times, combining the filtrates and removing the ethanol by vacuum distillation; The concentration treatment specifically includes: placing the organically extracted extract in a vacuum rotary apparatus and concentrating it in a water bath at a constant temperature of 60° C. until the relative density reaches 1.18 to 1.

25.

6. Use of the plant extract for treating cloacal inflammation in breeder roosters as claimed in any one of claims 1 to 3 in a preparation for preventing and treating cloacal inflammation in breeder roosters.

7. The use according to claim 6, characterized in that The cloacal inflammation of the breeder rooster is characterized by damage to the outside of the cloaca and bleeding or purulent secretions; or no damage to the outside of the cloaca but bleeding or purulent secretions.

8. The use according to claim 6, characterized in that The preparation is a spray preparation sprayed on the lesion site outside the cloaca or a spray preparation injected into the lesion site of the cloaca.

9. The use according to claim 7, characterized in that For cloacal inflammation in breeding roosters with damage to the outside of the cloaca and bleeding or purulent secretions, spray the damaged, bleeding or purulent secretions once a day with a spray volume of 0.5 to 1 ml each time for 3 to 5 consecutive days.

10. The use according to claim 8, characterized in that For cloacal inflammation in breeder roosters with no damage to the outside of the cloaca but bleeding or purulent secretions, insert the flat nozzle of the drug administration into the cloaca of the breeder rooster for spraying treatment, spray once a day, with a spray volume of 0.3~0.5 ml each time, and spray continuously for 3~5 days.

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