Traditional Chinese medicine composition with whitening and anti-aging effects, mask containing traditional Chinese medicine composition and preparation method

Abstract

The invention relates to the technical field of biology, in particular to a traditional Chinese medicine composition with whitening and anti-aging effects, a mask containing the traditional Chinese medicine composition and a preparation method. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 10 to 15 parts of dendrobium officinale, 10 to 15 parts of pomegranate fruit, 10 to 15 parts of haematococcus, 1.5 to 3 parts of lily, 1.5 to 3 parts of radix angelicae, 1.5 to 3 parts of fructus amomi, 0.9 to 4 parts of rhizoma kaempferiae, 2 to 6 parts of bletilla striata, 2 to 4.2 parts of rhizoma nardostachyos, 1.5 to 3.5 parts of fructus broussonetiae, 1.5 to 3.5 parts of rhizoma cimicifugae, 1.5 to 2.5 parts of mung bean and 2 to 9 parts of fructus gleditsiae. The obtained mask has the effects of moisturizing, resisting oxidation, resisting aging, inhibiting bacteria, whitening and the like, is free of irritation, does not have anaphylaxis, does not detect toxic heavy metal ions such as lead, mercury and arsenic in heavy metals, and completely meets the national standard.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, and in particular to a traditional Chinese medicine composition with whitening and anti-aging effects, a facial mask containing the composition, and a preparation method thereof. Background Art

[0002] At present, many brands have launched Chinese medicine combination mask products on the market, some of which focus on the inheritance of traditional ancient prescriptions, and some are developed by combining modern extraction technology and skin care concepts. Compared with some chemically synthesized skin care ingredients, Chinese medicine combinations are mostly derived from natural Chinese medicinal materials, which are relatively less irritating to the skin and suitable for a variety of skin types. In particular, if people with sensitive skin choose Chinese medicine masks with appropriate formulas, they can also take care of their skin to a certain extent. At the same time, Chinese medicine combination masks can improve skin conditions from multiple aspects with the help of the synergistic effects of multiple Chinese medicines, rather than solving a single skin problem. For example, they can not only hydrate and moisturize, but also improve the skin's glossiness and overall health at the same time.

[0003] With the development of the traditional Chinese medicine industry, traditional Chinese medicine-related facial masks emerge in an endless stream. For example, invention patent CN112006961A discloses a method for preparing an acne-removing traditional Chinese medicine facial mask essence and an acne-removing traditional Chinese medicine facial mask. The main ingredient of the mask is nine-headed lion grass, which solves the shortcomings of existing antibiotics for treating acne, such as easy drug resistance, easy recurrence, high price, harsh use conditions, and inability of patients with allergic constitution to use them on their own; it fills the market gap of traditional Chinese medicine acne-removing facial masks for severe youthful acne (acne), and has high social and economic benefits. Invention patent CN106039022B discloses a skin-lightening and whitening Chinese medicine composition, skin-lightening and whitening Chinese medicine preparation, skin-lightening and whitening facial mask, and preparation method. The Chinese medicine composition is composed of the following raw materials in parts by weight: 2-4 parts of forsythia fruit extract, 3-5 parts of siler extract, 2-4 parts of safflower extract, 3-5 parts of licorice root extract, 2-4 parts of angelica extract, 2-4 parts of salvia miltiorrhiza extract, 2-4 parts of lithospermum root extract, 2-4 parts of bletilla striata extract, 3-5 parts of peach kernel extract, 3-5 parts of ginkgo extract, and 1-3 parts of water-soluble pearl powder. The Chinese medicine composition can be used to prepare skin-lightening and whitening cosmetics. The facial mask prepared by the Chinese medicine composition has synergistic effects of various active ingredients, and the addition of moisturizers and other adjuvants assists the skin in transdermal absorption of the active ingredients in the Chinese medicine composition, so that the prepared facial mask has a significant therapeutic effect on dull and dull skin and spots.

[0004] Based on the above research direction, the inventor obtained a Chinese medicine composition with antioxidant and antibacterial effects during the research process, and prepared it into a facial mask. The obtained facial mask has an in vitro moisturizing rate of 96.92%, a DPPH free radical scavenging rate of 91.14%, a tyrosinase inhibition rate of 86.09%, and a chicken embryo chorioallantoic membrane anti-irritation score of ES = 0, which is basically non-irritating, without abnormal phenomena such as bleeding, coagulation, and vascular melting; when the 12 groups of facial masks are restored to room temperature after high temperature or freezing, there is no significant difference between the 12 groups of facial masks and before treatment; the antibacterial rate of Staphylococcus aureus is 94.87%, and the antibacterial rate of Escherichia coli is 98.27%; no allergic reaction was found in the skin test of 30 volunteers, and the irritation level was 0. Toxic heavy metal ions such as ions of lead, mercury and arsenic in heavy metals were also not detected. In summary, it fully meets the national standards. Summary of the invention

[0005] The primary purpose of the present invention is to provide a traditional Chinese medicine composition with whitening and anti-aging effects. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 10-15 parts of dendrobium officinale, 10-15 parts of pomegranate fruit, 10-15 parts of haematococcus, 1.5-3 parts of lily, 1.5-3 parts of angelica dahurica, 1.5-3 parts of amomum, 0.9-4 parts of galangal, 2-6 parts of bletilla striata, 2-4.2 parts of nard, 1.5-3.5 parts of mulberry fruit, 1.5-3.5 parts of cimicifuga heracleifolia, 1.5-2.5 parts of mung bean, and 2-9 parts of sapodilla.

[0006] Preferably, the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 10-15 parts of Dendrobium officinale, 10-15 parts of pomegranate fruit, 10-15 parts of Haematococcus pluvialis, 1.5-3 parts of lily, 1.5-3 parts of Angelica dahurica, 1.5-3 parts of Amomum villosum, 0.9-4 parts of Kaempferia galanga, 2-6 parts of Bletilla striata, 2-4.2 parts of Nardostachys grandiflora, 1.5-3.5 parts of Cuscuta australis, 1.5-3.5 parts of Cimicifuga heracleifolia, 1.5-2.5 parts of Mung Bean, and 2-9 parts of Gleditsia sinensis.

[0007] The second purpose of the present invention is to provide a Chinese herbal extract with whitening and anti-aging effects, wherein the Chinese herbal extract is prepared by extracting effective ingredients from the Chinese herbal composition as a raw material.

[0008] The third object of the present invention is to provide a facial mask with whitening and anti-aging effects, wherein the facial mask comprises the traditional Chinese medicine composition, a water phase and an oil phase.

[0009] Preferably, the aqueous phase comprises the following raw materials in parts by weight: 5-7 parts of niacinamide, 10-20 parts of glutathione, 4-6 parts of coenzyme Q10, 3-5 parts of vitamin C, 5-7 parts of hyaluronic acid, 5-7 parts of trehalose, 6-8 parts of small molecule soybean active peptides, 6-8 parts of arbutin, and 200 parts of distilled water (ml); the oil phase comprises the following raw materials in parts by weight: 30-50 parts of cetearyl alcohol, 10-20 parts of ethylparaben, 50-70 parts of glycerin, 3-5 parts of vitamin E, and 3-5 parts of vitamin A.

[0010] A fourth object of the present invention is to provide a method for preparing the facial mask, comprising the following steps:

[0011] (1) Mix the components in the oil phase according to the proportion, stir, and heat to sterilize;

[0012] (2) mixing the extracts of the Chinese medicine compositions according to a certain proportion, and filtering to obtain an extract;

[0013] (3) adding each component in the aqueous phase into water according to the proportion, stirring evenly, and heating to sterilize;

[0014] (4) When the temperature of the oil phase obtained in step (1) drops to 50° C., the oil phase is added to the water phase obtained in step (3), and heated and stirred to obtain a base liquid; when the temperature of the base liquid rises to 70° C., the extract obtained in step (2) is added to the base liquid, stirred, the heating is stopped, the base liquid is cooled to room temperature, and stirred again to be uniform.

[0015] Preferably, the heating temperature in step (1) is 80°C.

[0016] Preferably, the heating temperature in step (3) is 80°C.

[0017] Solution:

[0018] Dendrobium candidum: It tastes sweet and is slightly cold in nature. It has many important functions such as nourishing yin and clearing heat, benefiting the stomach and promoting the production of body fluids. In this formula combination, it can be used as the main nourishing medicine, and plays a key role in improving the body's yin deficiency and other conditions. It can be regarded as one of the main medicines. It is rich in antioxidant ingredients such as polysaccharides, flavonoids, and phenolic acids. These substances can efficiently capture and neutralize free radicals, have antioxidant power, delay the aging process of the skin, keep the skin firm and plump for a long time, and reduce fine lines, nasolabial folds and other signs of aging. It promotes the discharge of melanin in the skin, improves the dullness and spots caused by the accumulation of melanin, and makes the skin color more even and fair.

[0019] Red pomegranate fruit: rich in various nutrients, with antioxidant effects, it can play a major role in beauty and other aspects, and plays a role as a partial monarch medicine. Among them, it is rich in water and various vitamins, which can provide sufficient moisture and nutrition for the skin and keep the skin moisturized. The antioxidant ingredients such as red pomegranate polyphenols and anthocyanins in pomegranates can effectively neutralize free radicals and slow down the aging of the skin. Pomegranates also have certain anti-inflammatory and antibacterial effects, which can inhibit the growth of bacteria such as Staphylococcus aureus and hemolytic streptococci, and can assist in preventing skin problems such as acne and acne. Ingredients such as ellagic acid and polyphenols in pomegranate fruits can inhibit the production of melanin, reduce the occurrence of spots, and at the same time can also dilute existing spots, making the skin whiter and more even.

[0020] Haematococcus pluvialis: It contains ingredients such as astaxanthin, which has strong antioxidant capacity. It can play a leading role in regulating the body's state and fighting oxidative stress, and can also be used as a main drug. The released astaxanthin can efficiently capture and remove free radicals, slow down the oxidation rate of the skin, prevent the premature appearance of signs of aging, and keep the skin firm and elastic. Astaxanthin inhibits the release of inflammatory factors and reduces the inflammatory response of the skin. For people with sensitive skin and acne, it can effectively soothe the skin and reduce the frequency of skin allergies and acne.

[0021] Lily: It has the effects of moistening the lungs and relieving coughs, clearing the heart and calming the mind. It assists the main medicine in nourishing yin and moistening the lungs, and is a ministerial medicine. It contains a variety of active ingredients that can inhibit the activity of tyrosinase and reduce the synthesis of melanin, thereby reducing the chances of problems such as spots and dullness, making the skin brighter and fairer. The vitamin C, flavonoids and other ingredients in lily have antioxidant effects, which can remove free radicals in the body and reduce the damage of free radicals to skin cells, thereby delaying the aging process of the skin and preventing the appearance of wrinkles, sagging and other aging phenomena.

[0022] Angelica dahurica: It can dispel wind and relieve pain, reduce swelling and discharge pus, etc. It assists the main drugs in improving the body's blood circulation and relieving pain. It is a ministerial drug. Specifically, it has the effect of inhibiting the growth of various pathogenic bacteria, promoting skin metabolism, and delaying skin aging. Angelica dahurica can also activate blood circulation, discharge pus and promote muscle growth, and is helpful for whitening the skin.

[0023] Bletilla striata: It has the effects of astringing and stopping bleeding, reducing swelling and promoting tissue regeneration, etc. It helps the main medicine to improve the skin, promote wound healing or repair some internal injuries of the body. It plays an auxiliary therapeutic role and can treat skin diseases such as acne, tinea corporis, and scars. Because Bletilla striata contains rich starch, glucose, volatile oil and mucus, it can eliminate the traces of acne on the face, and can moisturize and whiten the skin, making the skin smooth as jade.

[0024] Amomum villosum: It can remove dampness and stimulate appetite, warm the spleen and stop diarrhea, regulate the spleen and stomach functions, prevent other drugs from being too greasy and affecting the spleen and stomach, and play a supporting role; it has the effects of removing acne, whitening, and removing marks, and also has astringent, antibacterial, and antioxidant effects. The microparticles in Amomum villosum can absorb the oil and dirt on the surface of the skin, effectively clean the pores, and reduce the occurrence of acne. The rich vitamin C and polyphenols in Amomum villosum can inhibit the production of melanin and make the skin whiter. The microparticles in Amomum villosum can slightly grind the surface of the skin, help remove melanin precipitation, and fade spots and marks. The tannic acid component in Amomum villosum has an astringent effect, can shrink pores, and make the skin tighter. The flavonoids, flavonoid glycosides, and tannic acid in Amomum villosum have antibacterial effects, can inhibit bacterial growth, and reduce the occurrence of acne. The polyphenols in Amomum villosum have antioxidant effects, can reduce the damage of free radicals, and protect skin health.

[0025] Kaempferia: It has the functions of promoting qi and warming the middle, digesting food, relieving pain, helping to regulate gastrointestinal function, and assisting other drugs to better exert their efficacy in regulating qi and blood, and is an adjuvant; it is rich in amino acids and vitamin E, so it has a good moisturizing effect. Kaempferia can penetrate into the skin and increase the water retention capacity between cells, so as to keep the skin hydrated and soft. The vitamin C and collagen in kaempferia can effectively resist oxidation and reduce the damage of free radicals to the skin, thereby slowing down the aging process. Using kaempferia products can make the skin firmer and smoother and delay the aging process of the skin. The vitamin C in kaempferia is a natural whitening agent, which can inhibit the production of melanin, even out skin color, and increase the brightness of skin color. Using kaempferia products can reduce the production of spots and freckles, making the skin whiter and brighter.

[0026] Cuscuta australis: It has the functions of nourishing the kidney and clearing the liver, improving eyesight, and promoting diuresis. It helps regulate the functions of the body's internal organs and plays the role of adjuvant medicine. It is rich in antioxidant ingredients such as flavonoids and phenolic acids. These substances can strongly capture and remove free radicals in the skin. It can delay skin aging, maintain skin firmness and elasticity, and reduce fine lines, crow's feet and other signs of aging. Cuscuta australis has a certain inhibitory effect on tyrosinase, reduces melanin production, helps to fade freckles, sun spots and other spots, brightens the overall skin tone, and improves the whiteness of the skin.

[0027] Cimicifuga: It has the functions of dispelling rashes, clearing away heat and detoxifying, and raising Yang Qi, helping to regulate the body's Qi, and assisting the main and secondary drugs in detoxification, as an adjuvant drug; it has the effect of removing spots and whitening. The various compounds in Cimicifuga can inhibit the formation of melanin and reduce the formation of spots, thereby achieving the effect of removing spots and whitening. The various active ingredients in Cimicifuga can resist oxidation and eliminate free radicals, thereby delaying skin aging. The compounds in Cimicifuga have certain anti-inflammatory and antibacterial effects, which can fight skin inflammation, acne and other problems.

[0028] Mung bean: It has the functions of clearing away heat and detoxifying. It plays an auxiliary role in preventing the drug from overheating or detoxifying in the whole formula, and is an adjuvant. The antioxidant ingredients contained in it can effectively capture free radicals, delay the skin's oxidation process, maintain the skin's elasticity and luster, and prevent premature aging. Mung bean has an inhibitory effect on tyrosinase, can reduce the production of melanin, help to lighten freckles, sun spots, chloasma and other spots, and make the skin color more even and bright.

[0029] Soap beans: They have the functions of expectoration and cough relief, opening the orifices and clearing the blockages, etc. Here, they can help guide the drugs to the diseased area or coordinate the overall movement of the drugs, playing the role of making the drugs work. They have certain antibacterial ability, and can inhibit the growth of common bacteria and fungi on the skin, such as Propionibacterium acnes and Malassezia. Soap beans maintain the stability of the skin's microbial environment and keep the skin healthy by antibacterial and anti-inflammatory effects. They are especially suitable for patients with acne and seborrheic dermatitis.

[0030] Nardostachys galanga: It has the effects of regulating qi and relieving pain, relieving depression and invigorating the spleen. It plays a role in harmonizing various drugs and guiding the drugs to better exert their comprehensive efficacy. It is a guiding drug; it has a regulating effect on the secretion of sebaceous glands and can reduce excessive secretion of skin oil. It can remove free radicals in the skin. Free radicals are the culprits that accelerate skin aging and promote wrinkles. Nardostachys galanga inhibits free radical activity, thereby delaying the natural aging process of the skin and maintaining the firmness and elasticity of the skin.

[0031] The beneficial effects of the present invention are as follows: the present invention provides a Chinese medicine composition with moisturizing, anti-oxidation, anti-aging, antibacterial, whitening and other effects, and the composition is prepared into a facial mask, and the obtained facial mask has an in vitro moisturizing rate of 96.92%, a DPPH free radical scavenging rate of 91.14%, a tyrosinase inhibition rate of 86.09%, a chicken embryo chorioallantoic membrane anti-irritation score of ES=0, and is basically non-irritating, without abnormal phenomena such as bleeding, coagulation and vascular melting, etc., when the facial masks of 12 groups are restored to room temperature after high temperature or freezing, there is no significant difference between the facial masks before treatment, the antibacterial rate of Staphylococcus aureus is 94.87%, and the antibacterial rate of Escherichia coli is 98.27%, and no allergic reaction is found in the skin test of 30 volunteers, and the irritation level is 0. Toxic heavy metal ions such as ion lead, mercury and arsenic in heavy metals are also not detected, and in summary, it fully meets the national standards. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The actual picture of the prepared mask

[0033] Figure 2 Moisturizing test of masks with different formulas

[0034] Figure 3 Detection of DPPH free radical scavenging rate of masks with different formulations

[0035] Figure 4 Detection of tyrosinase inhibitory activity of face masks with different formulations

[0036] Figure 5 Phenotype of control-treated chick embryos Note: The model control group was 1-2a before 1% SDS treatment, 1-2b after 1% SDS treatment, the solvent control group was 3-4a before saline treatment, 3-4b after saline treatment.

[0037] Figure 6 Phenotypes of chicken embryos treated with masks of different formulas Note: 1-9a are untreated chicken embryo blood vessels, 1-9b are chicken embryo blood vessels with added mask samples; 1 is mask formula 1, 2 is mask formula 2, 3 is mask formula 3, 4 is mask formula 7, 5 is mask formula 8, 6 is mask formula 9, 7 is mask formula 10, 8 is mask formula 11, and 9 is mask formula 12.

[0038] Figure 7 Anti-Escherichia coli test of masks with different formulations

[0039] Figure 8 Anti-Staphylococcus aureus test of masks with different formulations

[0040] Fig. 9 Mask irritation test Note: 1 is the saline control group, 2-5 are the mask test groups, and 4 volunteers are randomly selected from 30 volunteers. DETAILED DESCRIPTION

[0041] The protection scope of the present invention is described in detail below in conjunction with specific implementation methods. It should be noted that the protection scope of the present invention is not limited by the following embodiments.

[0042] In the following examples, unless otherwise specified, the methods described are conventional methods and can be obtained from the literature; the reagents and instruments used can be purchased from the market.

[0043] Yujisan comes from the "Surgery Authentic" of the Ming Dynasty. It is a prescription for beauty and skin care summarized by ancient doctors after long-term practice. It is composed of 6 grams each of Angelica dahurica, talc, and Rhizoma Aconiti Lateralis Preparata, and 120 grams of mung bean powder. It has the effects of clearing away heat and detoxifying, improving skin quality, brightening skin tone and skin health. In the present invention, the inventor takes Yujisan as the basis, combines modern pharmaceutical knowledge and skin science theory, and obtains a Chinese medicine composition with better moisturizing, anti-oxidation, anti-aging, antibacterial, whitening and other effects by adding and subtracting prescriptions.

[0044] In the present invention, the Dendrobium officinale was purchased from Yunnan, the lily was purchased from Shaanxi Zhiqi Biotechnology Co., Ltd., the Angelica dahurica, Amomum villosum, Kaempferia galanga, Bletilla striata, Nardostachys grandiflora, Cuscuta australis, Cimicifuga heracleifolia, Mung bean, Gleditsia sinensis, and pomegranate seeds were all purchased from Anguo Yuyanfang Chinese Herbal Medicine Co., Ltd., and the Haematococcus pluvialis was purchased from Fulekang Biotechnology Co., Ltd.

[0045] At present, enzymatic hydrolysis, acid-base and organic solvent extraction methods can be used, but for safety reasons and cost considerations, most of them use enzyme extraction methods, and a small number use ethanol extraction. At present, the main enzymatic hydrolysis methods mainly use cellulase, pectinase and papain, but the extraction efficiency of papain for alkaloids and polysaccharides is low, and the other two enzymes have similar extraction efficiencies. This study uses cellulase.

[0046] Example 1: Preparation of a Chinese medicine facial mask with whitening and anti-aging effects

[0047] 1 Research Methods

[0048] 1.1 Preprocessing

[0049] Citric acid (C6H8O7 H2O) and trisodium citrate were added to appropriate amounts of water, mixed, and the pH values ​​were adjusted to 5, 6, and 7 to prepare PBS buffer with good buffering effect.

[0050] Processing of Chinese herbal medicines: The purchased Chinese herbal medicines are crushed and ground, and impurities are sieved with a sieve, and the powder is obtained using a 12, 24, 60, 80 or even 200 mesh sieve as required.

[0051] 1.2 Preparation of Dendrobium officinale extract

[0052] Weigh some powder of Dendrobium officinale, add some PBS buffer (solid-liquid ratio is 1:25g / ml), mix thoroughly, add some cellulase (powder: enzyme = 1000:1), mix well, fix in a constant temperature oscillator, and enzymolyze for 2h at 55℃ (oscillation speed is 150r·min -1 ), take it out from the constant temperature oscillator, place it in 90℃ water to inactivate the enzyme for 10 minutes, cool it to room temperature, transfer it to a centrifuge tube and centrifuge it for 20 minutes (4000r·min -1 ), pour off the supernatant, wash the precipitate with 10 mL of water, centrifuge for 10 min, wash twice, and combine the supernatant.

[0053] 1.3 Preparation of extracts of other ingredients

[0054] 1.3.1 Extraction of lily

[0055] Weigh some lily powder, add some PBS buffer (solid-liquid ratio is 1:30g / ml), mix thoroughly, add some cellulase (powder: enzyme = 500:1), mix well, fix in a constant temperature oscillator, and enzymolyze at 55℃ for 2h (oscillation speed is 150r·min -1 ), take it out from the constant temperature oscillator, place it in 90℃ water to inactivate the enzyme for 10 minutes, cool it to room temperature, transfer it to a centrifuge tube and centrifuge it for 20 minutes (4000r·min -1), pour off the supernatant, wash the precipitate with 10 mL of water, centrifuge for 10 min, wash twice, and combine the supernatant.

[0056] 1.3.2 Extraction of Angelica dahurica

[0057] Take 500g of Angelica dahurica, crush it with a grinder, pass it through a 150-mesh sieve, take 45g of sample powder, use ethanol as the extraction solution, add 150ml of anhydrous ethanol, seal tightly, weigh accurately, and ultrasonically treat (power 300W, frequency 45kHz) for 1h, cool to room temperature, weigh twice the amount of anhydrous ethanol, and precipitate for 36h. Then centrifuge the mixture at 4000r / min for 1h, absorb the supernatant, put it into a rotary evaporator, evaporate it at 50℃ until there is no ethanol smell, and finally prepare a solution with a mass concentration of 1g / mL for standby use (processed in equal proportions).

[0058] 1.3.3 Extraction of Amomum villosum

[0059] Weigh some Amomum villosum powder, add some PBS buffer (solid-liquid ratio is 1:25 g / ml), mix thoroughly, add some cellulase (powder: enzyme = 100:1), mix well, fix in a constant temperature oscillator, and enzymolyze for 2 h at 55 ° C (oscillation speed is 150 r·min -1 ), take it out from the constant temperature oscillator, place it in 90℃ water to inactivate the enzyme for 10 minutes, cool it to room temperature, transfer it to a centrifuge tube and centrifuge it for 20 minutes (4000r·min -1 ), pour off the supernatant, wash the precipitate with 10 mL of water, centrifuge for 10 min, wash twice, and combine the supernatant.

[0060] 1.3.4 Extraction of Kaempferia galanga

[0061] After drying the galangal in an oven at 60°C for 2 h, it was crushed and passed through a 40-mesh sieve. 15 g of galangal powder was weighed and soaked in anhydrous ethanol solution at a solid-liquid ratio of 1:20 (g / mL) for 30 min. The mixture was then ultrasonically extracted at an ultrasonic power of 250 W for 45 min, vacuum filtered, and the extraction was repeated 3-4 times. The extracts were combined and placed in a rotary evaporator and recovered under reduced pressure at 60°C until there was no alcohol taste, thereby obtaining the total extract.

[0062] 1.3.5 Extraction of Bletilla striata

[0063] Weigh some Bletilla striata powder, add some PBS buffer (solid-liquid ratio is 1:40 g / ml), mix thoroughly, add some cellulase (powder: enzyme = 600:1), mix well, fix in a constant temperature oscillator, and enzymolyze at 55°C for 2 h (oscillation speed is 150 r·min -1), take it out from the constant temperature oscillator, place it in 90℃ water to inactivate the enzyme for 10 minutes, cool it to room temperature, transfer it to a centrifuge tube and centrifuge it for 20 minutes (4000r·min -1 ), pour off the supernatant, wash the precipitate with 10 mL of water, centrifuge for 10 min, wash twice, and combine the supernatant.

[0064] 1.3.6 Extraction of Nardostachys grandiflora

[0065] Weigh some nard powder, add some PBS buffer (solid-liquid ratio is 1:35 g / ml), mix thoroughly, add some cellulase (powder: enzyme = 1000:1), mix well, fix in a constant temperature oscillator, and enzymolyze for 2 h at 55 ° C (oscillation speed is 150 r / min) -1 ), take it out from the constant temperature oscillator, place it in 90℃ water to inactivate the enzyme for 10 minutes, cool it to room temperature, transfer it to a centrifuge tube and centrifuge it for 20 minutes (4000r·min -1 ), pour off the supernatant, wash the precipitate with 10 mL of water, centrifuge for 10 min, wash twice, and combine the supernatant.

[0066] 1.3.7 Extraction of Paper mulberry seeds

[0067] Weigh 1.0 g of paper mulberry powder, add 40 mL of pH 5.0 PBS buffer (solid-liquid ratio of 1:25 g / ml), mix thoroughly, add 0.01 g of cellulase, mix well, fix in a constant temperature oscillator, and enzymolyze at 60 ° C for 2 h (oscillation speed of 150 r·min -1 ), take it out from the constant temperature oscillator, place it in 90℃ water to inactivate the enzyme for 10 minutes, cool it to room temperature, transfer it to a centrifuge tube and centrifuge it for 20 minutes (4000r·min -1 ), pour off the supernatant, wash the precipitate with 10 mL of water, centrifuge for 10 min, wash twice, and combine the supernatant

[0068] 1.3.8 Extraction of Cimicifuga

[0069] Weigh 1.0 g of Cimicifuga powder, add 40 mL of pH 5.0 PBS buffer (solid-liquid ratio of 1:30 g / ml), add 0.01 g of cellulase, mix well, fix in a constant temperature oscillator, and enzymolyze at 60 ° C for 2 h (oscillation speed of 150 r·min -1 ), take it out from the constant temperature oscillator, place it in 90℃ water to inactivate the enzyme for 10 minutes, cool it to room temperature, transfer it to a centrifuge tube and centrifuge it for 20 minutes (4000r·min -1 ), pour off the supernatant, wash the precipitate with 10 mL of water, centrifuge for 10 min, wash twice, and combine the supernatant 1.3.9 Extraction of mung bean

[0070] Weigh some mung bean powder, add some PBS buffer (solid-liquid ratio is 1:30g / ml-1), mix thoroughly, add some cellulase (powder: enzyme = 500:1), mix well, fix in a constant temperature oscillator, and enzymolyze at 60℃ for 2h (oscillation speed is 150r·min -1 ), take it out from the constant temperature oscillator, place it in 90℃ water to inactivate the enzyme for 10 minutes, cool it to room temperature, transfer it to a centrifuge tube and centrifuge it for 20 minutes (4000r·min -1 ), pour off the supernatant, wash the precipitate with 10 mL of water, centrifuge for 10 min, wash twice, and combine the supernatant

[0071] 1.3.10 Extraction of Gleditsia sinensis

[0072] Take 2g of saponin powder, add 200mL of distilled water, mix thoroughly, add 0.04g of cellulase, mix well, extract in a water bath at 80-90℃ for 2-4h, take it out of the water bath and cool it to room temperature, then put it into a centrifuge and centrifuge it at 4000r / min for 20min. Collect the supernatant, add anhydrous ethanol to the supernatant, refrigerate it in a refrigerator at 4℃ for 24h, centrifuge it again at 4000r / min for 20min, take the supernatant and put it into a rotary evaporator, evaporate it at 60℃ until there is no ethanol smell.

[0073] 1.4 Preparation of Dendrobium officinale mask

[0074] 1.4.1 Mask ingredients and proportions

[0075] Table 1 Mask formula

[0076]

[0077]

[0078]

[0079] 1.4.2 Operation

[0080] The components and formulas of each mask are shown in Table 1. According to the proportion of each component, cetearyl alcohol, ethylparaben, glycerin, vitamin E, and vitamin A are mixed and stirred evenly according to their respective proportions, stirred and heated to 80°C, and maintained for 1 hour for sterilization to obtain the corresponding oil phase;

[0081] The extracts of Dendrobium candidum, pomegranate fruit, Haematococcus pluvialis, lily, angelica dahurica, Amomum villosum, Kaempferia galanga, Bletilla striata, Nardostachys grandiflora, Cuscuta australis, Gleditsia sinensis, Mung bean and Cimicifuga heracleifolia are mixed in respective proportions, filtered and placed in a liquid medicine storage tank, and the liquid is used as the extract.

[0082] Add nicotinamide, glutathione, coenzyme Q10, vitamin C, hyaluronic acid, trehalose, small molecule soybean active peptides, arbutin, etc. into water according to their respective proportions, stir evenly, heat to 80°C, maintain for 1 hour for sterilization, and obtain the corresponding water phase;

[0083] When the temperature of the oil phase drops to 50°C, add the oil phase to the water phase and heat and stir to obtain the base liquid; when the temperature of the base liquid rises to 70°C, add the extract to the base liquid and continue stirring for 30 minutes, stop heating and cool to room temperature, and stir evenly to obtain the Dendrobium officinale composite facial mask.

[0084] 1.5 Physical and chemical quality testing of Dendrobium officinale mask

[0085] 1.5.1 Properties and fineness

[0086] Extract 10g of mask samples from the container, put them in a container, and carefully examine their appearance characteristics; take 0.5g of mask samples from the container, gently massage them onto the arm, and detect their smoothness through vision, smell, touch and other senses.

[0087] 1.5.2 Determination of in vitro moisture retention

[0088] Fix the medical breathable tape on the culture dish to simulate human skin. At room temperature, apply the mask on the glass dish fixed with the medical breathable tape, weigh them together and record them as M0. Then place them indoors at 18-26℃ for about 12 hours, take out the breathable tape and culture dish, and immediately weigh the medical breathable tape and glass dish together to accurately weigh the mass of each sample and record them as Mn. Calculate the moisturizing rate according to formula (1):

[0089]

[0090] Where: Mn is the mass of the sample after 12 hours, g;

[0091] M0 is the mass of the sample before placement, g.

[0092] 1.5.3 Heat and cold resistance test

[0093] Take 100g of facial mask sample and put it into 10 test tubes of 20mL, put 10g of facial mask sample into each test tube, bake the mouth of the test tube with an alcohol lamp for 1min, then seal the mouth of the test tube with multiple layers of gauze, and place them in incubators with different temperatures, and set the temperature to 10-50℃ (every 5℃ is one step); at the same time, set the temperature of another group of incubators to 0 to -20℃ (every 5℃ is one step), and place the test tubes with samples in each incubator, keep them for 12-24h, take out the incubator, and cool them to room temperature. After they return to room temperature, observe the samples and compare their appearance and status with whether there is obvious difference before putting them in.

[0094] 1.5.4 Tyrosinase inhibitory activity assay

[0095] Prepare PBS (phosphate buffered saline) with a pH of 6.8, 1.0 mg / mL L-tyrosine solution, and 0.1 mg / mL tyrosinase solution. Add them to the test tubes in the following proportions;

[0096] Test sample 1 (i.e. A1): PBS: tyrosinase solution: L-tyrosine solution = 4:1:1;

[0097] Test sample 2 (i.e. A2): PBS: tyrosinase solution = 5:1;

[0098] Test sample 3 (i.e. A3): sample solution: PBS: tyrosinase solution: L-tyrosine solution = 1:3:1:1;

[0099] Test sample 4 (ie A4): sample solution: PBS: tyrosinase solution = 1:4:1.

[0100] After shaking and mixing the liquid in each test tube, place 1 to 4 test samples in each test tube in a 37°C water bath for about 15 minutes, take them out immediately and measure the absorbance of each test sample at 475 nm (A1 to A4). Calculate the tyrosinase inhibition rate (I) of the mask according to formula (2).

[0101] I=[1-(A3-A4) / (A1-A2)]×100% (2)

[0102] 1.5.5 Chicken embryo chorioallantoic membrane anti-irritation test

[0103] Sodium dodecyl sulfate (SDS) can cause certain damage to the chick embryo chorioallantoic membrane (CAM) and blood vessels (such as bleeding, vascular melting and coagulation). The anti-irritation effect of the sample is evaluated by comparing the score difference of the chorioallantoic membrane change index caused by adding the sample and not adding the sample. After adding the sample, the lighter the change and the lower the score, the better the anti-irritation effect of the sample.

[0104] Test materials: Sanhuang fertilized chicken embryos, sodium dodecyl sulfate (SDS), and normal saline (Shijiazhuang Siyao Co., Ltd.).

[0105] Experimental equipment: LRH-250 biochemical incubator (Shanghai Yiheng Scientific Instrument Co., Ltd.), Xiaocaowu H10 humidifier (Xiaocaowu Electric Co., Ltd.), microscope BM2000 (Nanjing Jiangnan Yongxin Optics Co., Ltd.).

[0106] Test sample: sample liquid of the day.

[0107] Experimental group settings:

[0108] A negative control group (0.10% SDS), a solvent control group (normal saline), a model control group (1% SDS), and a sample group (with facial mask solution) were set up respectively.

[0109] Experimental process: CAM (Chicken Embryo Chorioallantoic Membrane) preparation:

[0110] After 6-9 days of culturing the chicken embryo, use a strong flashlight to inspect the eggs, use professional dental sawtooth and hard iron curved forceps to peel off the eggshell of the air chamber part to expose the white egg membrane, and operate carefully and carefully to avoid large-scale damage to the integrity of the egg membrane. Use a pipette to drop a few drops of physiological saline solution to moisten the eggshell membrane, and carefully use hard iron curved forceps to remove the eggshell membrane to ensure that the blood vessels and vascular membranes are not damaged. After processing, observe under a microscope, observe the structure of the vascular system at this time, and judge its integrity and suitability for the experiment.

[0111] End point evaluation method operation:

[0112] 1 mL of the test substance was directly applied to the CAM. After 3 minutes of action, microscopic photography was used to observe and record the degree of changes in vascular toxicity, namely, the degree of vascular bleeding, coagulation, and vascular melting, and scores were given according to the severity.

[0113] a. Six chicken embryos were used as the test substances in the negative control group (0.10% SDS), six chicken embryos were used as the test substances in the model control group (1% SDS), six chicken embryos were used as the test substances in the sample group, and six chicken embryos were used as the solvent control group (normal saline).

[0114] b. Evaluation of test results:

[0115] Endpoint score (ES) calculation formula:

[0116] ES = the average of the sum of scores for the degree of bleeding, coagulation and vascular melting observed in the chick embryo

[0117] Table 2 Scoring and phenomena

[0118]

[0119] c. End point scoring method result evaluation:

[0120] d. When using the endpoint evaluation method for testing, the endpoint score (ES) should be accurately calculated and the result should be retained to two decimal places to ensure the accuracy and reliability of the scoring result:

[0121] e. Classify the irritation of the test substances to the chick chorioallantoic membrane (CAM) and blood vessels according to the ES values ​​in Table 3.

[0122] Table 3 End point scoring method results evaluation

[0123]

[0124] 1.5.6 Scavenging effect of facial mask on DPPH free radicals

[0125] Use an analytical balance to accurately weigh 788.0 mg DPPH (1,1-diphenyl-2-trinitrophenylhydrazine free radical), then use anhydrous ethanol to make a volume of 1000 mL and mix evenly, that is, a 2mmol / LDPPH solution, which is diluted 10 times to obtain a 0.2mmol / LDPPH solution for use. Take 2.0 mL of this solution and measure the A0 value at 517 nm. The mask (1.0 mL) and DPPH solution (3.0 mL) are fully mixed in the same way, and the absorbance At is measured at 517 nm after fully reacting in the dark for 30 minutes. The sample solution (1.0 mL) is mixed with anhydrous ethanol (3.0 mL), and its absorbance Ab is measured at 517 nm. The scavenging rate (S) of the mask for DPPH is calculated according to formula (3).

[0126] S=1-[(At-Ab) / A0]×100% (3)

[0127] 1.5.7 Antibacterial ability test of facial mask

[0128] Determine the inhibitory effect of the mask solution on microorganisms (bacteria Escherichia coli and Staphylococcus aureus). Take 10μl of the mask sample solution, add 1mL of LB culture medium and 10μl of bacterial solution, mix evenly, add to a sterile 96-well plate, use an ELISA reader to measure the absorbance value at 650mm, read and save the data, then seal the surface of the 96-well plate with plastic wrap and place it in a constant temperature incubator for 24h, set the incubator temperature to 37℃, take it out after 24h of incubation, measure the absorbance value again and save the data. Calculate the antibacterial rate according to the formula, formula 4 is as follows:

[0129] Antibacterial rate (%) = [1-(AD)] / (BC) × 100% (4)

[0130] Where: A: absorbance value of the experimental group after 24h of culture

[0131] B: Absorbance value of blank control group after 24h of culture

[0132] C: absorbance value of blank control group 24 hours ago

[0133] D: Absorbance value of the experimental group before 24h of culture

[0134] The experiment used two common bacteria, Escherichia coli and Staphylococcus aureus, to determine the antibacterial properties of the solution.

[0135] 1.5.8 Irritation test of facial mask

[0136] Thirty volunteers aged between 18 and 24 were selected. The liquid to be tested was dropped onto the patch test paper and applied to the skin of the subject's arm with the matching sticker. The palm of the hand was gently pressed to make it evenly adhere to the skin. After 24 hours, the patch tester was removed and the skin condition of the wound was observed. The identification standards are shown in Table 4.

[0137] Table 4 Identification standards for facial mask irritation

[0138]

[0139] 2 Results and discussion

[0140] 2.1 Evaluation of sensory performance of facial masks

[0141] Dendrobium officinale composite masks prepared according to the 12 formulations in the table, such as Figure 1 As shown, the color is earthy yellow or Naples yellow, and the overall appearance is maltose-like, exuding a strong Chinese herbal medicine flavor. The paste is delicate and soft, easy to apply, skin-friendly, without any tingling sensation, and without any granularity. According to the relevant provisions of the "People's Republic of China Cleaning Industry Standard" QB / 2872-2007, the appearance and aroma meet the sensory indicators in the document.

[0142] 2.2 Determination of in vitro moisture retention

[0143] The moisturizing data of 9 groups of facial masks with 12 formulas are shown in Table 5. The average moisturizing values ​​of the facial masks are as follows: Figure 2 As shown, it can be seen that as the formula of the mask samples changes, the difference in water evaporation rate is not obvious (Δ<0.015). There is no significant difference between the formulas, formula 8 has the highest water evaporation rate, and formula 1 has the lowest water evaporation rate. Therefore, all 12 kinds of Dendrobium officinale masks have good water retention effects.

[0144] Table 5 Moisturizing properties of masks with different formulations

[0145]

[0146]

[0147] 2.3 Scavenging rate of facial mask on DPPH free radicals

[0148] Free radicals will continue to damage collagen and elastic fibers in skin cells, which is a key factor in causing wrinkles, sagging, and skin aging. Antioxidants can effectively remove free radicals, delay the skin's oxidation process, maintain skin elasticity and radiance, and prevent premature aging. The free radical scavenging rate also reflects the antioxidant capacity of antioxidants. The 9 sets of data on the DPPH free radical scavenging rate of 12 kinds of mask formulas are shown in Table 6, and the average DPPH free radical scavenging rate is shown in Table 6. Figure 3 It can be seen that as the proportion of mask samples changes, the DPPH free radical scavenging rate varies significantly. Formula 12 is quite different from Formula 1. The scavenging rate of DPPH free radicals by Formula 12 is the highest, i.e. 92.30%, while that of Formula 1 is the lowest, i.e. 58.13%. The scavenging rates of DPPH free radicals by Formula 1 and Formula 12 differ significantly, with a difference of 34.17%. The average scavenging rates of DPPH free radicals by the 12 kinds of Dendrobium officinale masks are all above 55%, and all have good antioxidant effects, among which Formula 12 has the best antioxidant effect.

[0149] Table 6 DPPH free radical scavenging rate of 12 kinds of mask formulas

[0150]

[0151] 2.4 Tyrosinase inhibitory activity assay

[0152] Tyrosinase is a key enzyme in the synthesis of melanin. Inhibiting the activity of this enzyme can reduce the production of melanin, help to lighten spots such as freckles and sun spots, brighten the overall skin tone, and improve the whiteness of the skin. The inhibitory activity of tyrosinase in 12 facial masks is shown in Table 7. The average inhibitory activity of tyrosinase in different facial masks is shown in Figure 4 It can be seen that as the proportion of the mask samples of the 12 formulas changes, the inhibition rate of tyrosinase is not significantly different. Among them, the inhibition rate of tyrosinase in formula 1 is the highest, 91.73%, and the lowest is 79.97% in formula 6. The difference in the inhibitory activity of tyrosinase between formula 1 and formula 6 is quite significant, with a difference of 11.76% in the inhibitory activity of tyrosinase. The inhibition rates of all 12 Dendrobium officinale masks on tyrosinase are above 75%, so they all have good whitening effects, among which formula 1 has the best whitening effect.

[0153] Table 7 Determination of tyrosinase inhibitory activity of 12 kinds of facial masks

[0154]

[0155] 2.5 Chicken embryo chorioallantoic membrane anti-irritation test

[0156] 2.5.1 Control group treated with chicken embryos

[0157] like Figure 5 As shown in the results, it can be found that after adding the model control group (1% SDS), the chicken embryos showed obvious bleeding ( Figure 5-1 b, 2b), there was no significant difference in the chick embryos before and after adding the solvent control group (normal saline), and no bleeding occurred ( Figure 5-3 b, 4b), indicating that the solvent control group (normal saline) was non-irritating.

[0158] 2.5.2 Treatment of chicken embryos with facial masks of different recipes

[0159] Figure 6 In the figure, 1a is the chicken embryo blood vessels before adding mask formula 1, 1b is the chicken embryo blood vessels after adding mask formula 1, and there is no bleeding in the blood vessels; 2a is the chicken embryo blood vessels before adding mask formula 2, 2b is the chicken embryo blood vessels after adding mask formula 2, and there is no bleeding in the blood vessels; 3a is the chicken embryo blood vessels before adding mask formula 3, 3b is the chicken embryo blood vessels after adding mask formula 3, and there is no bleeding in the blood vessels; 4a is the chicken embryo blood vessels before adding mask formula 7, 4b is the chicken embryo blood vessels after adding mask formula 7, and there is no bleeding in the blood vessels; 5a is the chicken embryo blood vessels before adding mask formula 8, 5b is the chicken embryo blood vessels After adding mask formula 8, there was no bleeding in the blood vessels; 6a was the chicken embryo blood vessels before adding mask formula 9, 6b was the chicken embryo blood vessels after adding mask formula 9, there was no bleeding in the blood vessels; 7a was the chicken embryo blood vessels before adding mask formula 10, 7b was the chicken embryo blood vessels after adding mask formula 10, there was no bleeding in the blood vessels; 8a was the chicken embryo blood vessels before adding mask formula 11, 8b was the chicken embryo blood vessels after adding mask formula 11, there was no bleeding in the blood vessels; 9a was the chicken embryo blood vessels before adding mask formula 12, 9b was the chicken embryo blood vessels after adding mask formula 12, there was no bleeding in the blood vessels. Comprehensive analysis shows that mask formulas 1, 2, 3, 7, 8, 9, 10, 11, and 12 are non-irritating to chicken embryos.

[0160] 2.6 Antibacterial test of facial mask

[0161] Table 8 Antibacterial property test of facial mask

[0162]

[0163]

[0164] Note: Different letters indicate significant differences

[0165] Mask formula 3 had the lowest antibacterial rate against E. coli at 28.30%, followed by mask formula 10 at 56.83%; mask formula 1 had the highest antibacterial rate against E. coli at 121.73% (Table 8, Figure 7 ).

[0166] Mask formula 5 had the lowest antibacterial rate of 58.80% against Staphylococcus aureus, followed by mask formulas 9 and 10 with antibacterial rates of 74.80% and 75.80% against Staphylococcus aureus; while mask 3 had the highest antibacterial rate of 100.00% against Staphylococcus aureus (Table 8, Figure 8 ).

[0167] The comprehensive evaluation shows that the antibacterial rate of mask formula 11 is the highest, with an antibacterial rate of 98.27% against Escherichia coli and 94.87% against Staphylococcus aureus, followed by mask formulas 1 and 8, both of which have high antibacterial rates.

[0168] 2.7 Trial survey of Dendrobium officinale mask

[0169] After a systematic investigation and research, we obtained 100 questionnaires about the products, which covered multiple indicators, such as appearance, skin-friendliness, water retention, comfort, wipeability, and skin adsorption. We used the arithmetic mean method to make the evaluation and recorded it in Table 9.

[0170] Table 9 Questionnaire survey statistics

[0171]

[0172] Note: The evaluation results in the table are based on a 5-point system, which is excellent, good, fair, poor, and very poor, that is, 5 to 1

[0173] As can be seen from Table 9, mask formula 11 is more popular among volunteers than the other 11 formulas. Formula 11 is rated relatively high. After using it, most volunteers feel that the viscosity is suitable, the skin adsorption capacity is good, the mask is easy to apply, and the moisturizing feeling is good. The product appearance is also highly rated. The next best experience is mask formula 8 and 12.

[0174] 2.7 Mask irritation test

[0175] 2.7.1 Normal saline control group

[0176] Normal saline was used as the solvent control group, and the irritation level was 0 before and after 24 hours of the patch test.

[0177] 2.7.2 Facial mask experimental group

[0178] Table 10 Irritation test of facial mask

[0179]

[0180] Using saline as the solvent control group and Formula 11 mask as the test group, the patch test paper with mask and saline was applied to the skin of the subjects' forearms near the wrists. After 24 hours, the subjects' skin was observed for allergic reactions. The results showed that none of the 30 volunteers had adverse reactions, so the irritation level of Formula 11 mask was 0 ( Fig. 9 ).

[0181] Comprehensive analysis shows that the 11th formula mask has the best overall effect.

[0182] Formula ingredients and proportions: 15g of Dendrobium candidum, 15g of pomegranate fruit, 15g of Haematococcus pluvialis, 2g of lily, 2g of Angelica dahurica, 2g of Amomum villosum, 1.2g of Kaempferia galanga, 4g of Bletilla striata, 2.8g of Nardostachys grandiflora, 2g of Cucurbita chussima, 3g of Cimicifuga heracleifolia, 2g of Mung bean, 6g of Gleditsia sinensis, 7g of niacinamide, 70ml of glycerin, 5g of Vitamin C, 5g of Vitamin E, 5g of Vitamin A, 20g of glutathione, 6g of Coenzyme Q10, 7g of Hyaluronic acid, 7g of Trehalose, 8g of small molecule soybean active peptide, 8g of arbutin, 50g of Cetearyl Alcohol, 20g of Ethylparaben and 200ml of distilled water.

[0183] Comprehensive evaluation of the 11th formula mask: in vitro moisturizing rate 96.92%, DPPH free radical scavenging rate 91.14%; tyrosinase inhibition rate 86.09%; chicken embryo chorioallantoic membrane anti-irritation score ES = 0, basically non-irritating, no abnormal phenomena such as bleeding, coagulation and vascular melting; when restored to room temperature after high temperature or freezing, there is no significant difference between the 12 groups of formula masks and before treatment; Staphylococcus aureus inhibition rate is 94.87%, Escherichia coli inhibition rate is 98.27%; 30 volunteers skin tests found no allergic reactions, and the irritation level is 0. Toxic heavy metal ions such as ions of lead, mercury and arsenic in heavy metals were not detected. In summary, it fully meets the national standards.

[0184] In summary, the present invention provides a Chinese medicine composition with moisturizing, anti-oxidation, anti-aging, antibacterial, whitening and other effects, and prepares the Chinese medicine composition into a facial mask. The obtained facial mask has an in vitro moisturizing rate of 96.92% and a DPPH free radical scavenging rate of 91.14%; the tyrosinase inhibition rate is 86.09%; the chicken embryo chorioallantoic membrane anti-irritation score ES=0 is basically non-irritating, and there are no abnormal phenomena such as bleeding, coagulation and vascular melting; when the facial masks of 12 groups are restored to room temperature after high temperature or freezing, there is no obvious difference between the facial masks before treatment; the antibacterial rate of Staphylococcus aureus is 94.87%, and the antibacterial rate of Escherichia coli is 98.27%; no allergic reaction was found in the skin test of 30 volunteers, the irritation level was 0, and toxic heavy metal ions such as ions of lead, mercury and arsenic in the heavy metals were also not detected. In summary, it fully meets the national standards.

Claims

1. A Chinese medicine composition with whitening and anti-aging effects, characterized in that: The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 10-15 parts of dendrobium officinale, 10-15 parts of pomegranate fruit, 10-15 parts of haematococcus, 1.5-3 parts of lily, 1.5-3 parts of angelica dahurica, 1.5-3 parts of amomum villosum, 0.9-4 parts of galangal, 2-6 parts of bletilla striata, 2-4.2 parts of nard, 1.5-3.5 parts of mulberry fruit, 1.5-3.5 parts of cimicifuga heracleifolia, 1.5-2.5 parts of mung bean, and 2-9 parts of sapodilla.

2. The Chinese medicine composition according to claim 1, characterized in that The traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 10-15 parts of dendrobium officinale, 10-15 parts of pomegranate fruit, 10-15 parts of haematococcus, 1.5-3 parts of lily, 1.5-3 parts of angelica dahurica, 1.5-3 parts of amomum villosum, 0.9-4 parts of galangal, 2-6 parts of bletilla striata, 2-4.2 parts of nard, 1.5-3.5 parts of mulberry fruit, 1.5-3.5 parts of cimicifuga heracleifolia, 1.5-2.5 parts of mung bean and 2-9 parts of sapodilla.

3. A Chinese herbal extract with anti-aging and whitening effects, characterized in that: The Chinese medicine extract is prepared by extracting effective ingredients from the Chinese medicine composition described in claim 1 or 2 as raw material.

4. A facial mask with anti-aging and whitening effects, characterized in that: The facial mask comprises the Chinese medicine composition as claimed in claim 1 or 2, a water phase and an oil phase.

5. The facial mask according to claim 4, characterized in that, The water phase comprises the following raw materials in parts by weight: 5-7 parts of niacinamide, 10-20 parts of glutathione, 4-6 parts of coenzyme Q10, 3-5 parts of vitamin C, 5-7 parts of hyaluronic acid, 5-7 parts of trehalose, 6-8 parts of small molecule soybean active peptides, 6-8 parts of arbutin, and 200 parts of distilled water ml; the oil phase comprises the following raw materials in parts by weight: 30-50 parts of cetearyl alcohol, 10-20 parts of ethylparaben, 50-70 parts of glycerin, 3-5 parts of vitamin E, and 3-5 parts of vitamin A.

6. The method for preparing the facial mask according to claim 5, characterized in that: The steps include: (1) Mix the components in the oil phase according to the proportion, stir, and heat to sterilize; (2) mixing the extracts of each Chinese medicine composition according to a certain proportion, and filtering to obtain an extract; (3) adding each component in the aqueous phase into water according to the proportion, stirring evenly, and heating to sterilize; (4) When the temperature of the oil phase obtained in step (1) drops to 50° C., the oil phase is added to the water phase obtained in step (3), and heated and stirred to obtain a base liquid; when the temperature of the base liquid rises to 70° C., the extract obtained in step (2) is added to the base liquid, stirred, the heating is stopped, the base liquid is cooled to room temperature, and stirred again to be uniform.

7. The method for preparing the facial mask according to claim 6, characterized in that: The heating temperature in step (1) and step (3) is 80°C.

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