Composition for promoting hair growth and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a composition for promoting hair growth and a preparation method thereof. The composition for promoting hair growth comprises a traditional Chinese medicine extract and wormwood essential oil, and the traditional Chinese medicine extract is prepared from, by weight, 10-20 parts of wormwood, 5-15 parts of ginger, 2-10 parts of angelica sinensis, 2-10 parts of cacumen biotae, 2-10 parts of ligusticum wallichii, 2-10 parts of radix paeoniae rubra, 2-10 parts of saffron crocus, 2-10 parts of peach kernels, 1-10 parts of fructus cnidii, 1-10 parts of borneol and 1-10 parts of begonia yunnanensis. 1 to 10 parts of pearl powder and 1 to 10 parts of lagotis brevituba. The composition disclosed by the invention can obviously promote hair growth and maintain the physiological structure stability of hair follicles, and has important significance on hair growth and hair follicle protection.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a composition for promoting hair growth and a preparation method thereof. Background Art

[0002] It is generally believed that hair loss is affected by many factors, including endocrine disorders, psychology, drugs, genetics, spirit, age, environment and season, etc. The direct physiological causes of hair loss include excessive secretion of male hormones, low metabolic function of hair follicles and hair bulbs, and bacterial infection of the scalp.

[0003] From the perspective of traditional Chinese medicine, hair loss is related to the liver, kidney, spleen and blood qi of the five internal organs of the human body. Most hair loss is caused by liver and kidney deficiency, insufficient qi and blood, loose pores, open pores, wind evil taking advantage of the opportunity to enter, wind and blood dryness, or qi stagnation and blood stasis blocking the collaterals, blood cannot nourish the hair, etc., which makes the hair roots empty and the hair falls out.

[0004] The current strategies for hair loss mainly include the use of anti-hair loss shampoo, glucocorticoids, etc. However, the above drug treatments have the following problems:

[0005] 1. Hair care products mainly play the role of strengthening hair roots, tightening and smoothing hair scales, moisturizing hair, and making the hair surface softer and more lustrous; most of them focus on prevention when it comes to hair loss issues and cannot effectively and specifically solve the problem of hair loss.

[0006] 2. Although minoxidil hair growth products are effective, they have a long usage cycle and require many precautions. Before use, you need to build up tolerance and pay attention to the concentration of the hair growth solution. In addition, in the early stages of use, some users will experience a period of hair loss due to differences in personal constitution and skin quality, resulting in a poor user experience.

[0007] 3. Treatments such as hair transplantation are costly and put a heavy burden on patients.

[0008] Therefore, based on this, the solution of the present invention is proposed. Summary of the invention

[0009] An object of the present invention is to provide a composition for promoting hair growth.

[0010] Another object of the present invention is to provide a method for preparing the above composition.

[0011] A composition for promoting hair growth according to a specific embodiment of the present invention comprises a Chinese herbal extract and wormwood essential oil, wherein the weight ratio of the Chinese herbal extract to the wormwood essential oil is 1:100;

[0012] The Chinese medicine extract is made of the following components by weight: 10-20 parts of astragalus, 5-15 parts of ginger, 2-10 parts of angelica, 2-10 parts of oriental arborvitae leaves, 2-10 parts of chuanxiong, 2-10 parts of red peony root, 2-10 parts of saffron, 2-10 parts of peach kernel, 1-10 parts of cnidium monnieri, 1-10 parts of borneol, 1-10 parts of crabapple, 1-10 parts of pearl powder, and 1-10 parts of short-tube rabbit ear grass.

[0013] According to a specific embodiment of the present invention, the composition for promoting hair growth is made of the following components by weight: 15 parts of astragalus, 10 parts of ginger, 5 parts of angelica, 5 parts of oriental arborvitae leaves, 5 parts of chuanxiong, 5 parts of red peony root, 5 parts of saffron, 5 parts of peach kernel, 2 parts of cnidium monnieri, 2 parts of borneol, 2 parts of crabapple, 2 parts of pearl powder, and 2 parts of short-tubed rabbit ear grass.

[0014] According to a specific embodiment of the hair growth promoting composition of the present invention, the Chinese medicine extract is made of the following components in parts by weight: 10 parts of astragalus, 5 parts of ginger, 2 parts of angelica, 2 parts of Platycladus orientalis leaves, 2 parts of Chuanxiong, 2 parts of red peony root, 2 parts of saffron, 2 parts of peach kernel, 1 part of cnidium monnieri, 1 part of borneol, 1 part of crabapple, 1 part of pearl powder, and 1 part of short-tubed rabbit ear grass.

[0015] According to a specific embodiment of the hair growth promoting composition of the present invention, the Chinese medicine extract is made of the following components in parts by weight: 20 parts of astragalus, 15 parts of ginger, 10 parts of angelica, 10 parts of Platycladus orientalis leaves, 10 parts of Chuanxiong, 10 parts of red peony root, 10 parts of saffron, 10 parts of peach kernel, 10 parts of cnidium monnieri, 10 parts of borneol, 10 parts of crabapple, 10 parts of pearl powder, and 10 parts of short-tubed rabbit ear grass.

[0016] Astragalus is sweet and slightly warm in nature. It enters the spleen and lung meridians. It has the effects of strengthening the spleen and replenishing the middle, raising yang and lifting the sunken, strengthening the body's defense and strengthening the exterior, promoting urination, and removing toxins and promoting tissue regeneration. It is mainly used to treat spleen deficiency syndrome, spontaneous sweating due to qi deficiency syndrome, and qi and blood deficiency.

[0017] Ginger is spicy and slightly warm in nature. It enters the lung, spleen, and stomach meridians. It has the effects of dispelling cold, warming the middle and stopping vomiting, warming the lungs and stopping cough, and detoxifying. It is used for colds caused by wind and cold, cold spleen and stomach syndrome, vomiting caused by cold stomach, and cough caused by cold lungs.

[0018] Angelica sinensis is sweet and spicy in taste and warm in nature. It enters the liver, heart and spleen meridians. It has the effects of nourishing blood, promoting blood circulation, regulating menstruation and relieving pain. It is mainly used to treat blood deficiency, sallow complexion, dizziness, palpitations, and pain caused by deficiency and cold.

[0019] Platycladus orientalis leaves are bitter, astringent and cold. They enter the lung, liver and spleen meridians. They have the effects of cooling blood and stopping bleeding, and promoting hair growth and darkening hair. They are used for hematemesis, epistaxis, hemoptysis, blood in the stool, metrorrhagia, cough due to lung heat, hair loss due to blood heat, and premature graying of hair.

[0020] Chuanxiong is pungent and warm in taste. It enters the liver, gallbladder and pericardium meridians. It has the effects of promoting blood circulation and qi, dispelling wind and relieving pain. It is used to treat headaches, chest pain, coronary heart disease, wind-cold-dampness arthralgia, and diseases caused by cerebrovascular circulation disorders.

[0021] Red peony root, bitter in taste, slightly cold in nature; enters the liver meridian. It has the effects of clearing heat and cooling blood, dispersing blood stasis and relieving pain. It is used for heat entering the blood, red and swollen eyes, liver depression and flank pain, abdominal pain due to lumps, injuries from falls, carbuncles, swellings and ulcers.

[0022] Saffron is sweet and neutral in nature. It enters the heart and liver meridians. It has the effects of promoting blood circulation and removing blood stasis, dispersing depression and relieving knots. It is used to treat depression, chest tightness, vomiting blood, typhoid fever, abdominal pain due to blood stasis, and swelling and pain due to falls.

[0023] Peach kernel is bitter, sweet and flat in nature and flavor; it enters the heart, liver and large intestine meridians. It has the effects of promoting blood circulation and removing blood stasis, moistening the intestines and relieving constipation, and relieving cough and asthma. It is used for amenorrhea, dysmenorrhea, lumps and masses, lung and intestinal abscesses, injuries from falls, dry intestines and constipation, and cough and asthma.

[0024] Cnidium monnieri, pungent, bitter, warm in nature; enters the kidney meridian. It dries dampness and dispels wind, kills insects and relieves itching, warms the kidney and strengthens yang. It is used for eczema itching, dampness and arthralgia, and impotence due to kidney deficiency.

[0025] Borneol tastes pungent and bitter, and is cool in nature. It enters the heart, spleen, and lung meridians. It has the effects of invigorating the mind, clearing away heat, and relieving pain. It is used for fever, coma, sudden syncope due to qi stagnation, coma due to poisoning, red eyes, and sore throat. It can also be used to treat the dryness and heat of comprehensive hot drugs.

[0026] The taste of the herb is bitter and pungent, and it is slightly warm in nature. It enters the liver, spleen, and kidney meridians. It has the effects of promoting qi and relieving pain, promoting blood circulation and removing blood stasis. It is used for stomachache, dysmenorrhea, vomiting and diarrhea in children, hernia, and traumatic injuries.

[0027] Pearl powder is sweet, salty and cold in nature. It enters the heart and liver meridians. It has the effects of calming the nerves, clearing the liver and improving eyesight, detoxifying and promoting tissue regeneration. It is used for palpitations, restlessness, insomnia, convulsions and epilepsy.

[0028] Short-tube rabbit ear grass, a traditional Tibetan medicine, tastes bitter and sweet, and is cold in nature. It enters the liver and lung meridians. It has the effects of clearing away heat and detoxifying, lowering blood pressure, and regulating menstruation. It is mainly used to treat nephritis, lung abscess, tuberculosis cough, hypertension, systemic fever, damp-heat diarrhea. It can also be used to detoxify drugs.

[0029] Wormwood is pungent, bitter, and warm in nature. It enters the liver, spleen, and kidney meridians. It warms the meridians and stops bleeding, dispels cold and relieves pain. It can also be used externally to remove dampness and relieve itching. External use of wormwood essential oil can kill bacteria, reduce inflammation, relieve itching, warm the meridians and dredge the collaterals, etc.

[0030] The preparation method of the Chinese medicine extract of the present invention is:

[0031] (1) decocting angelica, Platycladus orientalis leaves, Ligusticum chuanxiong, Paeonia lactiflora, Saffron, and peach kernel in water, wherein Astragalus and Cnidium monnieri are added 15 minutes before the end of decoction to obtain a decoction;

[0032] (2) The decoction is filtered through gauze to be concentrated, and the concentrated decoction is cooled; the effective ingredients of short-tube rabbit ear grass are extracted by alcohol extraction and water precipitation method to obtain short-tube rabbit ear grass extract; the oil components in mountain crabapple are extracted by distillation method to obtain mountain crabapple extract; volatile and non-volatile ginger oil in ginger are obtained by supercritical CO2 extraction method to obtain ginger extract; the short-tube rabbit ear grass extract, mountain crabapple extract, ginger extract and the decoction are mixed, and borneol, pearl powder and wormwood essential oil are added thereto, and stirred at a low speed at 35°C for 10 minutes to obtain a traditional Chinese medicine composition.

[0033] According to a specific embodiment of the present invention, the composition for promoting hair growth is prepared by the following steps:

[0034] (1) decocting angelica, Platycladus orientalis leaves, Ligusticum chuanxiong, Paeonia lactiflora, Saffron, and peach kernel in water, wherein Astragalus and Cnidium monnieri are added 15 minutes before the end of decoction to obtain a decoction;

[0035] (2) The decoction is filtered through gauze to be concentrated, and the concentrated decoction is cooled; the effective ingredients of short-tube rabbit ear grass are extracted by alcohol extraction and water precipitation method to obtain short-tube rabbit ear grass extract; the oil components in mountain crabapple are extracted by distillation method to obtain mountain crabapple extract; volatile and non-volatile ginger oil in ginger are obtained by supercritical CO2 extraction method to obtain ginger extract; the short-tube rabbit ear grass extract, mountain crabapple extract, ginger extract and the decoction are mixed, and borneol, pearl powder and wormwood essential oil are added thereto, and stirred at a low speed at 35°C for 10 minutes to obtain a traditional Chinese medicine composition.

[0036] Preferably, in step (2), the decoction is concentrated at 75-100°C.

[0037] The method of using the composition of the present invention is: after cleaning the scalp, evenly apply it on the scalp, and wash it off after half an hour. Use it twice a day for at least one month.

[0038] Beneficial effects of the present invention:

[0039] The present invention is based on the analysis of the basic theory of traditional Chinese medicine, and summarizes the pathogenesis of hair loss in modern people as deficiency of the root and excess of the superficial symptoms, wherein the root is: 1. long-term fatigue and insufficient rest lead to liver and kidney deficiency and insufficient qi and blood; qi deficiency leads to loose pores, that is, hair is easy to fall off; on the other hand, hair growth is weak, that is, hair growth is slow; blood deficiency cannot nourish hair, leading to yellow hair, thin and soft and easy to break; 2. long-term mental work, insufficient sleep, excessive worry, easy to hurt the spleen (spleen is responsible for thinking), hurt the heart, hurt the gallbladder, hurt the mind, leading to a large amount of hair falling off. The superficial symptoms are: 1. due to insufficient qi and blood, loose pores, open pores, wind evil takes advantage of the weakness to enter, wind is strong and blood is dry, and hair is damaged; 2. due to irregular work and rest, greasy or unseasoned diet, qi stagnation and blood stasis block collaterals, leading to blockage of hair follicles, and hair growth is not easy.

[0040] Based on this, the present invention proposes a composition for promoting hair growth, including Chinese medicine extract components and wormwood essential oil. In the extract, the main drugs of the whole formula are astragalus and angelica, which can strengthen the spleen and replenish qi, replenish blood and promote blood circulation; the auxiliary drugs are arborvitae leaves and red peony root, which can clear heat and cool blood, promote hair growth and black hair; chuanxiong and ginger can warm the interior and dispel cold, dispel wind and promote qi circulation; the adjuvant drugs are saffron and peach kernel, which can promote blood circulation and remove blood stasis, dispel depression and open knots; cnidium monnieri and borneol can dry dampness and dispel wind, warm the kidney and strengthen yang, open the mind and wake up the mind, and detoxify drugs; the auxiliary drugs are short-tube rabbit ear grass, mountain crabapple, and pearl powder to promote qi and calm the mind, dispel dryness and heat, and harmonize various drugs. And the wormwood essential oil is harmonized to enhance the auxiliary effects of sterilization and anti-inflammatory, antipruritic, warming the meridians and unblocking the collaterals, which can calm the nerves and relieve tension and anxiety, and assist the extract components to penetrate and absorb into the scalp.

[0041] The whole prescription is both offensive and tonifying. It starts with the main factors of modern hair loss, analyzes the etiology and pathogenesis, and formulates the prescription. In addition to traditional Chinese medicine, natural mineral drugs (pearls) and Tibetan medicine are added to the prescription. According to the medicinal properties and the effects of the active ingredients, a variety of modern extraction methods are used. The compatibility (five flavors) are complete, cold and hot are balanced, multiple points are effective, and the effect is synergistic. It promotes the regrowth of hair in patients with hair loss from multiple angles of "hair growth", "hair care", "hair development", and "hair prosperity".

[0042] Table 1 Analysis of the characteristics of the prescription according to the etiology and pathogenesis

[0043]

[0044]

[0045] Table 2 Analysis of the characteristics of the prescriptions according to the meridians

[0046] BRIEF DESCRIPTION OF THE DRAWINGS

[0047] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0048] Figure 1 Shown is a comparison of mouse hair growth.

[0049] Figure 2 Shows the results of HE staining of skin tissue in the hair-depleted area on the back of mice.

[0050] Figure 3 Counting of hair follicles in the back skin tissue of mice.

[0051] Figure 4 KI67 staining results of skin tissue in the hair-depleted area on the back of mice.

[0052] Figure 5 Results of immunofluorescence staining (tunel, Ki67) of mouse skin tissue.

[0053] Figure 6 Results of immunofluorescence staining (Nerf2, PARP) of mouse skin tissue.

[0054] Figure 7 Results of immunofluorescence staining (IL-4, IL-10) of mouse skin tissue.

[0055] Figure 8 Immunofluorescence staining of mouse skin tissue (macrophages, CD8 + T cell) results.

[0056] Fig. 9 Showing some of the patient effects. DETAILED DESCRIPTION

[0057] To make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other implementation methods obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0058] The methods of alcohol extraction and water precipitation, distillation, and supercritical CO2 extraction in the present invention are all conventional technical means in the field, and those skilled in the art can operate according to actual conditions.

[0059] Example 1

[0060] The composition of the present invention comprises a Chinese herbal medicine extract and wormwood essential oil, wherein the weight ratio of the Chinese herbal medicine extract to the wormwood essential oil is 100:1;

[0061] The Chinese medicine extract comprises: 15 parts of astragalus, 10 parts of ginger, 5 parts of angelica, 5 parts of orientalis leaves, 5 parts of ligusticum chuanxiong, 5 parts of red peony root, 5 parts of saffron, 5 parts of peach kernel, 2 parts of cnidium monnieri, 2 parts of borneol, 2 parts of begonia, 2 parts of pearl powder and 2 parts of short-tubed rabbit ear grass.

[0062] The preparation method of the composition of the present invention comprises the following steps:

[0063] (1) decocting angelica sinensis, Platycladus orientalis leaves, Ligusticum chuanxiong, Paeonia lactiflora, Saffron, and peach kernel to obtain a decoction, wherein Astragalus membranaceus and Cnidium monnieri are added 15 minutes before the end of the decoction;

[0064] (2) The decoction is filtered through gauze to be concentrated, and the concentrated decoction is cooled; the effective ingredients of short-tube rabbit ear grass are extracted by alcohol extraction and water precipitation method to obtain short-tube rabbit ear grass extract; the oil components in mountain crabapple are extracted by distillation method to obtain mountain crabapple extract; volatile and non-volatile ginger oil in ginger are obtained by supercritical CO2 extraction method to obtain ginger extract; the short-tube rabbit ear grass extract, mountain crabapple extract, ginger extract and the decoction are mixed, and borneol, pearl powder and wormwood essential oil are added thereto, and stirred at a low speed at 35°C for 10 minutes to obtain a traditional Chinese medicine composition.

[0065] Example 2

[0066] The composition of the present invention comprises a Chinese herbal medicine extract and wormwood essential oil, wherein the weight ratio of the Chinese herbal medicine extract to the wormwood essential oil is 1:100;

[0067] The Chinese medicine extract comprises: 10 parts of astragalus, 5 parts of ginger, 2 parts of angelica, 2 parts of oriental arborvitae leaves, 2 parts of ligusticum chuanxiong, 2 parts of red peony root, 2 parts of saffron, 2 parts of peach kernel, 1 part of cnidium monnieri, 1 part of borneol, 1 part of begonia, 1 part of pearl powder and 1 part of short-tubed rabbit ear grass.

[0068] The preparation method of the composition of the present invention comprises the following steps:

[0069] (1) decocting angelica sinensis, Platycladus orientalis leaves, Ligusticum chuanxiong, Paeonia lactiflora, Saffron, and peach kernel to obtain a decoction, wherein Astragalus membranaceus and Cnidium monnieri are added 15 minutes before the end of the decoction;

[0070] (2) The decoction is filtered through gauze to be concentrated, and the concentrated decoction is cooled; the effective ingredients of short-tube rabbit ear grass are extracted by alcohol extraction and water precipitation method to obtain short-tube rabbit ear grass extract; the oil components in mountain crabapple are extracted by distillation method to obtain mountain crabapple extract; volatile and non-volatile ginger oil in ginger are obtained by supercritical CO2 extraction method to obtain ginger extract; the short-tube rabbit ear grass extract, mountain crabapple extract, ginger extract and the decoction are mixed, and borneol, pearl powder and wormwood essential oil are added thereto, and stirred at a low speed at 35°C for 10 minutes to obtain a traditional Chinese medicine composition.

[0071] Example 3

[0072] The composition of the present invention comprises a Chinese herbal medicine extract and wormwood essential oil, wherein the weight ratio of the Chinese herbal medicine extract to the wormwood essential oil is 1:100;

[0073] The Chinese medicine extract comprises: 20 parts of astragalus, 15 parts of ginger, 10 parts of angelica, 10 parts of oriental arborvitae leaves, 10 parts of ligusticum chuanxiong, 10 parts of red peony root, 10 parts of saffron, 10 parts of peach kernel, 10 parts of cnidium monnieri, 10 parts of borneol, 10 parts of begonia, 10 parts of pearl powder and 10 parts of short-tubed rabbit ear grass.

[0074] The preparation method of the composition of the present invention comprises the following steps:

[0075] (1) decocting angelica sinensis, Platycladus orientalis leaves, Ligusticum chuanxiong, Paeonia lactiflora, Saffron, and peach kernel to obtain a decoction, wherein Astragalus membranaceus and Cnidium monnieri are added 15 minutes before the end of the decoction;

[0076] (2) The decoction is filtered through gauze to be concentrated, and the concentrated decoction is cooled; the effective ingredients of short-tube rabbit ear grass are extracted by alcohol extraction and water precipitation method to obtain short-tube rabbit ear grass extract; the oil components in mountain crabapple are extracted by distillation method to obtain mountain crabapple extract; volatile and non-volatile ginger oil in ginger are obtained by supercritical CO2 extraction method to obtain ginger extract; the short-tube rabbit ear grass extract, mountain crabapple extract, ginger extract and the decoction are mixed, and borneol, pearl powder and wormwood essential oil are added thereto, and stirred at a low speed at 35°C for 10 minutes to obtain a traditional Chinese medicine composition.

[0077] Example 4

[0078] Establishment of the experimental animal model: C57 mice were selected for model establishment. After one week of routine laboratory feeding, the back skin tissues of all mice were depilated. Use a small electric shaver to carefully shave the hair on the back of the mouse, and be careful not to damage the back skin to prevent the mouse from suffering skin damage and infection and death. The shaved area is about 3cm×4cm. According to the instructions for use of the depilatory cream, apply the depilatory cream to the skin area where the hair has been shaved, wait for 10 minutes after application, and then use a cotton ball or gauze soaked in physiological saline to wipe off the depilatory cream and residual hair. At this time, it can be observed that the skin on the back of the mouse is light pink.

[0079] The control group received no treatment, while the hair growth group (Example 1) applied drugs to the hair removal area every day and observed the hair growth.

[0080] On days 0, 5, 10, and 15 after hair removal, the hair removal area on the back of the mice was photographed. On the 15th day of the experimental treatment, the mouse skin was taken for pathological sections and H&E staining to observe the hair growth of the mice; the paraffin sections of the mouse back skin tissue were immunohistochemically stained for the cell proliferation index protein Ki67; the skin tissue specimens of each group of mice were made into paraffin sections and then HE and immunofluorescence staining were performed.

[0081] On the day of hair removal, the hair removal areas on the backs of mice in each group were pink or light gray, with no hair growth. On the 5th day after hair removal, a small amount of short hair growth was observed in the hair growth group, while there was basically no hair growth in the control group. On the 10th day, obvious hair growth was observed in some parts of the hair growth group, while the hair growth in the control group was less, with only a small amount of light gray hair. On the 15th day, the new hair in the hair growth group was shiny, black, and dense, while the new hair in the control group was less, with obvious hair removal boundaries, and the hair growth was not as good as that in the hair growth group ( Figure 1 );

[0082] On the 15th day of the experimental treatment, the mouse skin was taken for pathological sections and HE staining to observe the hair follicles of the mice. It can be seen that the number of hair follicles in the control group is relatively small. The results of horizontal comparison are shown in Figure 2 The growth of the hair follicles in the germination group was more than that in the control group. The statistics and comparison of the number of hair follicles in each group under a microscope (×20) Figure 3 ).

[0083] The paraffin sections of the mouse back skin tissue were immunohistochemically stained for the cell proliferation index protein Ki67. The positive staining results were brown-yellow particles. The darker the color, the more obvious it is, indicating that the more Ki67 protein is expressed, the more vigorous the cell proliferation and division. The results can be seen Figure 4The expression of KI67 protein in the hair follicle cells of the skin tissue in the hair growth group was significantly higher than that in the control group, indicating that the proliferation activity of hair follicle cells was promoted after hair growth treatment.

[0084] After the skin tissue specimens of each group of mice were made into paraffin sections, immunofluorescence staining was performed. The marker was the cell proliferation protein Ki67 (red), the cell nucleus was marked with DAPI (blue), and the apoptotic cells were stained with Tunel (green). The positive results of DAPI staining were blue, the positive results of Ki67 staining were red, and the positive results of Tunel staining were green. The darker and more obvious the red color of Ki67 staining, the more Ki67 protein was expressed, that is, the more vigorous the cell proliferation and division; the darker the green color of Tunel staining, the more cell apoptosis. The results of immunofluorescence staining were consistent with the results of immunohistochemistry ( Figure 5 ), the expression of KI67 protein in the hair follicle cells of the hair growth group was significantly higher than that in the control group, and there were basically no apoptotic cells in the hair growth group, which further confirmed that the hair growth prescription promoted the proliferation of hair follicle cells after treatment.

[0085] Immunofluorescence staining, the markers are cell DNA repair protein PARP (red), cell oxidative stress repair protein Nerf2 staining (green), and cell nuclei are marked with DAPI (blue). DAPI positive staining results are blue, PARP positive staining results are red, and Nerf2 positive staining results are green. The darker the red color of PARP staining, the more obvious it is, indicating that cell DNA damage repair significantly promotes hair follicle cell growth; the darker the green color of Nerf2 staining, the more the cell's antioxidant capacity increases, which can resist oxidative stress damage and accelerate the repair of hair follicles to oxidative stress ( Figure 6 ). The expression of Nerf2 and PARP proteins in the hair follicle cells of the hair growth group was significantly higher than that in the control group, which confirmed that the hair growth prescription treatment promoted the anti-oxidative stress damage and damage repair ability of hair follicle cells.

[0086] Immunofluorescence staining, the markers are anti-inflammatory factor IL-4 (red), anti-inflammatory factor IL-10 (green), and the cell nucleus is marked with DAPI (blue). DAPI positive staining results are blue, IL-4 positive staining results are red, and IL-10 positive staining results are green. The deeper and more obvious the IL-4 and IL-10 staining, the more obvious it is, indicating that the inflammatory state of hair follicle cells is suppressed ( Figure 7 ). The expression of IL-4 and IL-10 in the hair follicle cells of the skin tissue of the hair growth group was significantly higher than that of the control group, confirming that the hair growth prescription promoted the anti-inflammatory effect of hair follicle cells after treatment.

[0087] Immunofluorescence staining with marker CD8 +T cells (red), M1 macrophages (green) were labeled with the marker CD86, and the cell nuclei were labeled with DAPI (blue). DAPI positive staining results are blue, CD8 positive staining results are red, + T cells; CD86 staining positive results are green, M1 macrophages. The deeper the CD8 and CD86 staining, the more obvious it is, indicating that M1 macrophages (promoting the secretion of inflammatory factors) and CD8 + T cells (promote the secretion of inflammatory factors), ( Figure 8 ). M1 macrophages and CD8 + The T cell aggregation was significantly less than that in the control group, further confirming that the hair follicle inflammation was reduced after treatment with the hair growth formula.

[0088] Typical Cases

[0089] Two men used the Chinese medicine composition of Example 1 respectively. Dosage and method: After cleaning the scalp, apply it evenly on the scalp and wash it off after half an hour. Twice a day, 10g-15g each time. The usage period is 2 months.

[0090] like Fig. 9 As shown, the contrast effect before and after use is obvious, and the user's hair becomes thicker and darker.

[0091] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A composition for promoting hair growth, characterized in that: The composition comprises a Chinese herbal medicine extract and wormwood essential oil, wherein the weight ratio of the Chinese herbal medicine extract to the wormwood essential oil is 100:

1. The Chinese medicine extract is made of the following components by weight: 10-20 parts of astragalus, 5-15 parts of ginger, 2-10 parts of angelica, 2-10 parts of oriental arborvitae leaves, 2-10 parts of chuanxiong, 2-10 parts of red peony root, 2-10 parts of saffron, 2-10 parts of peach kernel, 1-10 parts of cnidium monnieri, 1-10 parts of borneol, 1-10 parts of crabapple, 1-10 parts of pearl powder, and 1-10 parts of short-tube rabbit ear grass.

2. The composition for promoting hair growth according to claim 1, characterized in that: The Chinese medicine extract is made of the following components by weight: 15 parts of astragalus, 10 parts of ginger, 5 parts of angelica, 5 parts of Platycladus orientalis leaves, 5 parts of Chuanxiong, 5 parts of red peony root, 5 parts of saffron, 5 parts of peach kernel, 2 parts of cnidium monnieri, 2 parts of borneol, 2 parts of crabapple, 2 parts of pearl powder, and 2 parts of short-tubed rabbit ear grass.

3. The composition for promoting hair growth according to claim 1, characterized in that: The Chinese medicine extract is made of the following components by weight: 10 parts of wormwood, 5 parts of ginger, 2 parts of angelica, 2 parts of Platycladus orientalis leaves, 2 parts of Chuanxiong, 2 parts of red peony root, 2 parts of saffron, 2 parts of peach kernels, 1 part of Cnidium monnieri, 1 part of borneol, 1 part of Begonia sabdariffa, 1 part of pearl powder, and 1 part of short-tubed rabbit ear grass.

4. The composition for promoting hair growth according to claim 1, characterized in that: The Chinese medicine extract is made of the following components by weight: 20 parts of astragalus, 15 parts of ginger, 10 parts of angelica, 10 parts of Platycladus orientalis leaves, 10 parts of Chuanxiong, 10 parts of red peony root, 10 parts of saffron, 10 parts of peach kernel, 10 parts of cnidium monnieri, 10 parts of borneol, 10 parts of crabapple, and 10 parts of short-tubed rabbit ear grass.

5. The composition for promoting hair growth according to claim 1, characterized in that: The composition is prepared by the following steps: (1) decocting angelica, Platycladus orientalis leaves, Ligusticum chuanxiong, Paeonia lactiflora, Saffron, and peach kernel in water, wherein Astragalus and Cnidium monnieri are added 15 minutes before the end of decoction to obtain a decoction; (2) The decoction is filtered through gauze to be concentrated, and the concentrated decoction is cooled; the effective ingredients of short-tube rabbit ear grass are extracted by alcohol extraction and water precipitation method to obtain short-tube rabbit ear grass extract; the oil components in mountain crabapple are extracted by distillation method to obtain mountain crabapple extract; volatile and non-volatile ginger oil in ginger are obtained by supercritical CO2 extraction method to obtain ginger extract; the short-tube rabbit ear grass extract, mountain crabapple extract, ginger extract and the decoction are mixed, and borneol, pearl powder and wormwood essential oil are added thereto, and stirred at a low speed at 35°C for 10 minutes to obtain a traditional Chinese medicine composition.

6. The composition for promoting hair growth according to claim 5, characterized in that: In step (2), the decoction is concentrated at 75-100°C.

7. The method for preparing the composition for promoting hair growth according to claim 1, characterized in that: The preparation method comprises the following steps: (1) decocting angelica sinensis, Platycladus orientalis leaves, Ligusticum chuanxiong, Paeonia lactiflora, Saffron, and peach kernel to obtain a decoction, wherein Astragalus membranaceus and Cnidium monnieri are added 15 minutes before the end of the decoction; (2) The decoction is filtered through gauze to be concentrated, and the concentrated decoction is cooled; the effective ingredients of short-tube rabbit ear grass are extracted by alcohol extraction and water precipitation method to obtain short-tube rabbit ear grass extract; the oil components in mountain crabapple are extracted by distillation method to obtain mountain crabapple extract; volatile and non-volatile ginger oil in ginger are obtained by supercritical CO2 extraction method to obtain ginger extract; the short-tube rabbit ear grass extract, mountain crabapple extract, ginger extract and the decoction are mixed, and borneol, pearl powder and wormwood essential oil are added thereto, and stirred at a low speed at 35°C for 10 minutes to obtain a traditional Chinese medicine composition.

8. The method for preparing the composition for promoting hair growth according to claim 8, characterized in that: In step (2), the decoction is concentrated at 75-100°C.

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