Liquid composition capable of simultaneously deodorizing and promoting plant growth as well as preparation method and application of liquid composition

Abstract

The invention discloses a liquid composition capable of simultaneously deodorizing and promoting plant growth as well as a preparation method and application of the liquid composition. The liquid composition is prepared from the following raw materials in parts by weight: 50-60 parts of camellia oleifera shells, 10-40 parts of citrus, 10-20 parts of lithocarpus polystachyus and 1-15 parts of zymophyte. The preparation method comprises the following steps: grinding citrus into residues, and filtering to obtain citrus juice which is liquid A; crushing camellia oleifera shells and lithocarpus polystachyus, and mixing the crushed camellia oleifera shells and lithocarpus polystachyus with water in a weight ratio of 1: (10-500) to obtain a mixed solution B; and mixing the mixed solution B and the solution A, adding zymophyte, mixing at 20-40 DEG C, sealing and fermenting for 10-20 days, and filtering the fermentation solution to obtain the feed additive. The agricultural and forestry residue camellia oleifera shells are fully utilized, value-added processing and utilization are expanded, the materials are simple and easy to obtain, the raw materials are low in price, the citrus deodorization effect is obvious, fermentation can be promoted, and the composition can also promote plant growth.

Description

Technical Field

[0001] The invention belongs to the technical field of processing and utilization of agricultural and forestry residues, and particularly relates to a liquid composition capable of simultaneously deodorizing and promoting plant growth, and a preparation method and application thereof. Background Art

[0002] The information disclosed in this background technology section is only intended to increase some understanding of the overall background of the invention, and should not be necessarily regarded as an admission or any form of suggestion that the information constitutes the prior art already known to ordinary technicians in this field.

[0003] Agricultural and forestry residues are important members of waste, an important biomass resource, and an important renewable resource. The energy conversion and utilization of agricultural and forestry residues is one of the research hotspots in the field of renewable energy. Camellia oleifera shells are byproducts of camellia oleifera fruit used to process tea oil. Studies have shown that camellia oleifera shells are rich in active substances such as lignin, polypentose, tea saponin and tannin, which can be used as bioenergy or applied in food and industrial fields. Tea saponin in camellia oleifera shells can repel pests and have a certain antibacterial effect. Tea saponin also contains rich carbon sources and can promote microbial fermentation. Microbial remediation is a controlled or spontaneous process that uses microorganisms to catalyze and degrade organic pollutants, thereby repairing the polluted environment or eliminating pollutants in the environment. Among them, microbial remediation technology uses microorganisms (indigenous bacteria, foreign bacteria, genetically engineered bacteria) to metabolize pollutants to transform and degrade pollutants. Tea saponin has a significant effect on improving the biodegradation efficiency of organic pollutants.

[0004] During the planting process, citrus fruits fall due to diseases or management. At present, there is little research on the technology of recycling defective fallen fruits, and the only way is to use the traditional deep burial treatment or pile composting. Studies have found that citrus fruits are rich in nutrients, contain a variety of biologically active substances, and are rich in vitamins, carotene, amino acids, flavonoids and other nutrients, and have a high resource utilization value. If the fallen citrus fruits are processed and utilized for value-added purposes, the income of citrus growers can be increased, which is friendly to the environment.

[0005] Sweet tea, commonly known as sweet tea, modern phytochemical research shows that the main biologically active ingredients in sweet tea are flavonoids and polysaccharides. Sweet tea is rich in polysaccharides, and sugar metabolism provides an important carbon source and energy for organisms, which can promote microbial fermentation.

[0006] Compost, especially manure, will produce odor during the decomposition process. The main raw materials of compost are organic matter, such as animal and plant residues, feces, etc. These organic substances will produce gas through the action of microorganisms during the decomposition process. Due to the respiration of microorganisms and the incomplete oxidation of organic matter, some odorous gases will be produced. The fermentation process of compost is a complex biochemical process. In this process, microorganisms obtain energy and generate metabolites by decomposing organic matter. These metabolites often contain odorous gases. The environment and climatic conditions in which compost is located will also affect its fermentation process. High temperature and high humidity environment are conducive to the reproduction and activity of microorganisms, thereby accelerating the decomposition of organic matter and producing more odorous gases. Some organic matter in manure contains sulfur, which may produce odorous gases such as hydrogen sulfide during the decomposition process. This is another important reason for the odor of compost. In order to reduce the generation of odor during composting, appropriate management measures can be taken, such as adjusting humidity, controlling temperature, adding biological agents to promote the growth of beneficial microorganisms, etc. These methods help to reduce the odor problem during composting.

[0007] Animal manure is often used in farms to make organic fertilizers, so the organic matter in livestock and poultry manure can be fully collected, and the nutrient utilization rate is high. However, the storage and fermentation cycle of manure generally takes more than half a year. How to use high-quality and inexpensive deodorizers to remove odors, speed up decomposition and promote plant growth has market prospects. Summary of the invention

[0008] In view of the above problems, the present invention provides a liquid composition that can deodorize and promote plant growth and a preparation method thereof. The liquid composition is prepared with oil tea shells, citrus, polysaccharide and microorganisms. A reasonable material-liquid ratio enables the present invention to extract and dissolve effective substances faster, and can also better remove odors from toilets, farms, etc., and can quickly ferment manure to promote plant growth.

[0009] The technical solution adopted by the present invention is as follows:

[0010] In a first aspect of the present invention, a liquid composition capable of simultaneously deodorizing and promoting plant growth is provided, wherein the liquid composition is made of the following raw materials in parts by weight:

[0011] 50-60 parts of camellia oleifera shells, 10-40 parts of citrus, 10-20 parts of lycopodii, and 1-15 parts of fermentation bacteria.

[0012] In one or some embodiments of the present invention, the citrus refers to citrus peels, citrus fallen fruits and other citrus plants and different parts, including different varieties.

[0013] In one or some embodiments of the present invention, the polystachya is one or more of its leaves, branches, and rhizomes.

[0014] In one or some embodiments of the present invention, the fermentation bacteria is a combination of saccharomyces cerevisiae, Lactobacillus plantarum and Bacillus velez. It has been verified through experiments that the fermentation bacteria composed of saccharomyces cerevisiae, Lactobacillus plantarum and Bacillus velez have excellent technical effects of deodorization and plant growth promotion, otherwise they do not have excellent deodorization and growth promotion effects. The fermentation bacteria are added in the form of bacterial liquid, containing an effective viable count of 5×10 9 ~20×10 9 cfu / mL, the ratio of the number of live bacteria in Saccharomyces cerevisiae, Lactobacillus plantarum, and Bacillus velezensis was (2-4):(6-8):(2-4).

[0015] In the present invention, the liquid fermented by the oil tea shell, polysaccharide and citrus in a reasonable ratio can quickly remove odors, and the addition of citrus can make the deodorant liquid treated by the present invention have a significant effect of removing odors without the need for other flavors.

[0016] In a second aspect of the present invention, a method for preparing the liquid composition capable of simultaneously deodorizing and promoting plant growth is provided, comprising the following steps:

[0017] (1) crushing the citrus into pulp, filtering, and obtaining the citrus juice as liquid A;

[0018] (2) crushing the oil-tea camellia shell and the polysaccharide, and mixing them with water in a weight ratio of 1:10 to 500 to obtain a mixed solution B;

[0019] (3) Mix the mixed liquid B and liquid A, add fermentation bacteria, mix and seal at 20-40° C. for fermentation for 10-20 days, and filter the fermented liquid to obtain the product.

[0020] In one or some embodiments of the present invention, the fermentation time is 15 days.

[0021] In the above preparation method, the fermentation bacteria can significantly increase the fermentation time of the material, and the fermentation temperature of the material is 20-40° C., which significantly improves the deodorization effect.

[0022] In the above preparation method, a method of solvent (water) extraction and microbial combined fermentation is adopted to rapidly ferment the plant raw materials, and a suitable proportion of Lithospermum multiflorum is added to improve the comprehensive performance of the fermentation.

[0023] In the third aspect of the present invention, there is provided use of the composition in preparing a product capable of simultaneously deodorizing and promoting plant growth.

[0024] In one or some embodiments of the present invention, the plant is Camellia oleifera.

[0025] Compared with the related art known to the inventor, one of the technical solutions of the present invention has the following beneficial effects:

[0026] The invention makes full use of tea oil shells, which are agricultural and forestry residues, to expand value-added processing and utilization. The materials are simple and easy to obtain, the raw materials are relatively cheap, the deodorizing effect is obvious, fermentation can be promoted, and the composition can also promote plant growth. The invention can quickly improve the dissolution of effective ingredients, and the ingredients are all stable, and can be better used for deodorizing toilets, farms, etc., and can also be applied to adding feces to quickly ferment into liquid fertilizer. The invention is natural and environmentally friendly, has no petrochemical derivatives, and the deodorizing effect is fresh and natural. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The accompanying drawings constituting a part of the present invention are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their description are used to explain the present invention and do not constitute improper limitations on the present invention.

[0028] Figure 1 : Camellia oleifera seedlings before applying the present invention.

[0029] Figure 2 The oil-tea camellia seedlings applied with the present invention three times had greener leaves and grew rapidly. DETAILED DESCRIPTION

[0030] It should be noted that the following detailed descriptions are exemplary and are intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0031] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit exemplary embodiments according to the present invention. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprising" and / or "including" are used in this specification, it indicates the presence of features, steps, operations and / or combinations thereof.

[0032] In order to enable those skilled in the art to more clearly understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below in conjunction with specific embodiments.

[0033] The brewer's yeast, Lactobacillus plantarum and Bacillus velezinii of the present invention can all be obtained through commercial channels.

[0034] Example 1

[0035] A liquid composition capable of deodorizing and promoting plant growth and a preparation method thereof, comprising the following raw materials in percentage by weight: 50% of camellia oleifera shell, 30% of citrus, 10% of polyspicate lychnophora, and 10% of fermentation bacteria.

[0036] (1) crushing the citrus into pulp, filtering, and obtaining the citrus juice as liquid A;

[0037] (2) Crush the oil-tea camellia shells and polyspicate lycopodii into 60 meshes;

[0038] (3) mixing the material from step (2) with water at a weight ratio of 1:50 to obtain a mixed solution B;

[0039] (4) Add fermentation bacteria to the mixture B and citrus juice A. The fermentation bacteria is a composite bacterial liquid of saccharomyces cerevisiae, Lactobacillus plantarum and Bacillus velez. The effective viable bacteria count is 10×10 9 cfu / mL, the ratio of the number of live bacteria in Saccharomyces cerevisiae, Lactobacillus plantarum and Bacillus velez was 2:6:3, and the mixture was sealed and fermented at 20°C for 15 days, and the fermentation liquid was filtered to obtain the product.

[0040] Example 2

[0041] A liquid composition capable of deodorizing and promoting plant growth and a preparation method thereof, comprising the following raw materials in percentage by weight: 60% of camellia oleifera shell, 30% of citrus, 5% of polyspicate lychnophora, and 5% of fermentation bacteria.

[0042] (1) crushing the citrus into pulp, filtering, and obtaining the citrus juice as liquid A;

[0043] (2) Crush the oil-tea camellia shells and polyspicate lycopodii into 60 meshes;

[0044] (3) mixing the material from step (2) with water in a weight ratio of 1:10 to obtain a mixed solution B;

[0045] (4) Add fermentation bacteria to the mixture B and citrus juice A. The fermentation bacteria is a composite bacterial liquid of saccharomyces cerevisiae, Lactobacillus plantarum and Bacillus velez. The effective viable bacteria count is 10×10 9 cfu / mL, the ratio of the number of live bacteria in Saccharomyces cerevisiae, Lactobacillus plantarum and Bacillus velez was 3:8:3, and the mixture was sealed and fermented at 40°C for 15 days, and the fermentation liquid was filtered to obtain the product.

[0046] Example 3

[0047] A liquid composition capable of deodorizing and promoting plant growth and a preparation method thereof, comprising the following raw materials in percentage by weight: 55% of camellia oleifera shell, 15% of citrus, 20% of polyspicate lychnophora, and 10% of fermentation bacteria.

[0048] (1) crushing the citrus into pulp, filtering, and obtaining the citrus juice as liquid A;

[0049] (2) Crush the oil-tea camellia shells and polyspicate lycopodii into 60 meshes;

[0050] (3) mixing the material from step (2) with water in a weight ratio of 1:100 to obtain a mixed solution B;

[0051] (4) Add fermentation bacteria to the mixture B and citrus juice A. The fermentation bacteria is a composite bacterial liquid of saccharomyces cerevisiae, Lactobacillus plantarum and Bacillus velez. The effective viable bacteria count is 10×10 9 cfu / mL, the ratio of the number of live bacteria in Saccharomyces cerevisiae, Lactobacillus plantarum and Bacillus velez was 4:6:3, and the mixture was sealed and fermented at 30°C for 15 days, and the fermentation liquid was filtered to obtain the product.

[0052] Example 4

[0053] A liquid composition capable of deodorizing and promoting plant growth and a preparation method thereof, comprising the following raw materials in percentage by weight: 60% of camellia oleifera shell, 35% of citrus, 10% of polyspicate lychnophora, and 5% of fermentation bacteria.

[0054] (1) crushing the citrus into pulp, filtering, and obtaining the citrus juice as liquid A;

[0055] (2) Crush the oil-tea camellia shells and polyspicate lycopodii into 60 meshes;

[0056] (3) mixing the material from step (2) with water in a weight ratio of 1:500 to obtain a mixed solution B;

[0057] (4) Add fermentation bacteria to the mixed solution C and citrus juice A. The fermentation bacteria is a composite bacterial solution of saccharomyces cerevisiae, Lactobacillus plantarum and Bacillus velez. The effective viable bacteria count is 10×10 9 cfu / mL, the ratio of the number of live bacteria in Saccharomyces cerevisiae, Lactobacillus plantarum and Bacillus velez was 3:8:2, and the mixture was sealed and fermented at 30°C for 15 days, and the fermentation liquid was filtered to obtain the product.

[0058] Comparative Example 1

[0059] It is basically the same as Example 1, except that the preparation raw material does not contain camellia oleifera shells, but is replaced with walnut shells in the same proportion. The results show that the deodorant containing camellia oleifera shells has a better deodorizing effect.

[0060] Comparative Example 2

[0061] It is basically the same as Example 1, except that the raw materials do not contain citrus and are replaced with grapefruit in the same proportion. The results show that the deodorant prepared with the raw materials containing citrus smells more comfortable.

[0062] Comparative Example 3

[0063] The method is basically the same as Example 1, except that the fermentation bacteria are changed to Lactobacillus plantarum and Bacillus subtilis (the ratio of live bacteria is 1:1), and the number of effective live bacteria is 10×10 9cfu / mL, the results show that the fermentation strain of the present invention is more suitable for fermenting the raw material of the present invention and fermenting feces. After adding citrus and polystigma, the taste is fresh and natural, and the deodorization effect is faster and better.

[0064] Comparative Example 4

[0065] It is basically the same as Example 1, except that the temperature of the fermentation raw material mixture is 10°C or 50°C. The results show that the material heating temperature at 20-40°C can increase the fermentation speed of the raw materials.

[0066] Comparative Example 5

[0067] It is basically the same as Example 1, except that the raw material does not contain Lithops multiflora, and is replaced by black tea in the same proportion. The results show that adding Lithops multiflora can promote fermentation.

[0068] Comparative Example 6

[0069] The method is basically the same as Example 1, except that the ratio of the number of live bacteria in Saccharomyces cerevisiae, Lactobacillus plantarum, and Bacillus velez is 1:1:1, and the number of effective live bacteria is 10×10 9 The cfu / mL is the same as that in Example 1. The results show that an inappropriate ratio of fermentation strains cannot better ferment the fermentation raw material of the present invention, resulting in poor deodorization and growth promotion effects.

[0070] Experimental Example 1: Effect of the present invention on the deodorization effect of ammonia water

[0071] Experimental steps:

[0072] ① Odor source preparation: Prepare 5% ammonia water, take out a 250ml triangular pyramidal bottle, put a 1cm*1cm filter paper in the bottle mouth, drop 0.05g of 5% ammonia water, cover with a rubber cap, let it stand for 5 minutes, and have 3 people smell the concentration, represented by A;

[0073] ②Put 0.5g of deodorant liquid made of different raw materials into the triangular pyramidal bottle in step ①, let it stand for 15 minutes, and then have the same three people smell the odor concentration represented by B;

[0074] ③ After the three people compared the odors A and B, they scored them according to the deodorization effect scoring indicators in Table 1.

[0075] The deodorization effect scoring index is shown in Table 1.

[0076] Table 1 Deodorization effect scoring index

[0077]

[0078]

[0079] Table 2-1 Comparison of deodorization effects of different raw materials

[0080]

[0081]

[0082]

[0083] From Table 2-1, it can be seen that the raw materials for preparing the deodorant containing oil tea shell, citrus, and polyspicate are all rated 3 for deodorization, while the deodorant effect of other raw materials as deodorants is poor. The deodorant prepared by the raw materials of the present invention has a more pleasant smell. At the same time, it can also be seen that the deodorization effect is different with different raw material addition ratios, indicating that the raw material addition ratio of the present invention is optimal.

[0084] Table 2-2 Comparison of deodorization effects of different raw materials fermentation time and fermentation temperature

[0085]

[0086]

[0087] It can be seen from Table 2-2 that different fermentation times and fermentation temperatures have different deodorizing effects on raw materials, indicating that the deodorizing effect of the raw materials of the present invention is best when the fermentation time is 15 days and the temperature is 20-40°C.

[0088] Table 3 Deodorization effect comparison between examples and comparative examples

[0089]

[0090] It can be seen from Table 3 that the deodorization scores of the embodiments are all 3, indicating that the deodorization effect of the embodiments of the present invention is better than that of the comparative example.

[0091] Experimental Example 2: Rapid deodorization effect of the strain of the present invention on fermented feces

[0092] Experimental method: 100 g of the fermented liquid of the embodiment and the comparative example were respectively put into 10 g of cow dung, and the fermentation was continued for 7 days. After the fermentation was completed, it was filtered, dried in a drying oven at 100°C for 24 hours, weighed, and the biomass, bacterial population and deodorization effect were observed.

[0093] Table 4 Effects of Examples and Comparative Examples on Fermented Feces

[0094]

[0095]

[0096] It can be seen from Table 4 that the biomass of the examples after fermenting feces is more than that of the comparative examples, and the deodorization scores are all 3, which illustrates the advantages of the present invention for fermenting feces.

[0097] Experimental Example 3: Effect of the present invention on promoting the growth of tea seedlings

[0098] 1.1 Overview of the trial site

[0099] Overview of the test site

[0100] The test site is the oil-tea nursery of Guangxi Academy of Forestry Sciences (108°21′E, 22°56′N), which has a subtropical monsoon climate; the average annual temperature is 21.7℃, the average annual minimum temperature is 12.8℃, and the average annual maximum temperature is 28.2℃; the average annual precipitation is 1300mm, and the average annual relative humidity is 79%; the summer is humid, the winter is slightly dry, and the dry and wet seasons are distinct. The soil conditions of the test site are total nitrogen (N) content of 1.80g / kg, total phosphorus (P) content of 0.10g / kg, and total potassium (K) content of 8.63g / kg.

[0101] 1.2 Test seedlings

[0102] The afforestation seedlings used in this experiment are fragrant camellia seedlings, provided by the Guangxi Zhuang Autonomous Region Forestry Science Research Institute. The eucalyptus seedlings in the test plot are fragrant camellia seedlings cultivated by the Guangxi Zhuang Autonomous Region Forestry Science Research Institute, with an average seedling height of 12cm and an average ground diameter of 2.54mm, and their size and growth conditions are basically the same.

[0103] 1.3 Management

[0104] After cutting on April 9, 2020, the cuttings were moved to the oil-tea nursery of Guangxi Academy of Forestry Sciences together with the planting cups. The compound fertilizer aqueous solution was applied every half month before the oil-tea seedling test for routine management. On March 11, 2021, the liquids of the embodiment and the comparative example were respectively irrigated on the oil-tea seedlings. 30 oil-tea seedlings were irrigated for each treatment, irrigated once every 10 days, for a total of 9 times, 3L was irrigated for each treatment each time, and 100mL was applied for each plant. Figure 1 , the oil tea seedlings before applying the present invention; Figure 2 The leaves of the tea seedlings to which the composition of this example was applied three times were greener and grew rapidly.

[0105] 1.4 Index determination

[0106] During the experiment, the growth of seedlings was monitored and the number of survivors, seedling height and ground diameter were recorded. The seedling height was measured with a tape measure (accurate value 0.01m); the ground diameter was measured with a vernier caliper (accurate value 0.01mm); and the number of survivors was counted directly.

[0107] Table 5

[0108]

[0109] Table 6 Effects of different treatments on the height and ground diameter of Camellia oleifera seedlings

[0110]

[0111]

[0112] Table 7 Effects of different treatments on the number of leaf buds and leaves in Camellia oleifera seedlings

[0113]

[0114] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A liquid composition capable of simultaneously deodorizing and promoting plant growth, characterized in that: The liquid composition is made from the following raw materials in parts by weight: 50-60 parts of camellia oleifera shells, 10-40 parts of citrus, 10-20 parts of lycopodii, and 1-15 parts of fermentation bacteria.

2. The liquid composition capable of simultaneously deodorizing and promoting plant growth as claimed in claim 1, characterized in that: The citrus refers to citrus peels, citrus fallen fruits and other parts of citrus plants.

3. The liquid composition capable of simultaneously deodorizing and promoting plant growth as claimed in claim 1, characterized in that: The polystachya is one or more of its leaves, branches, and rhizomes.

4. The liquid composition capable of simultaneously deodorizing and promoting plant growth as claimed in claim 1, characterized in that: The fermentation bacteria is a combination of saccharomyces cerevisiae, lactobacillus plantarum and bacillus velez.

5. The liquid composition capable of simultaneously deodorizing and promoting plant growth as claimed in claim 4, characterized in that: The fermentation bacteria are added in the form of bacterial liquid, containing 5×10 9 ~20×10 9 cfu / mL, the ratio of the number of live bacteria in Saccharomyces cerevisiae, Lactobacillus plantarum, and Bacillus velezensis was (2-4):(6-8):(2-4).

6. The method for preparing the liquid composition capable of simultaneously deodorizing and promoting plant growth according to any one of claims 1 to 5, characterized in that: The method comprises the following steps: (1) crushing the citrus into pulp, filtering, and obtaining the citrus juice as liquid A; (2) crushing the oil-tea camellia shell and the polysaccharide, and mixing them with water in a weight ratio of 1:10 to 500 to obtain a mixed solution B; (3) Mix the mixed liquid B and liquid A, add fermentation bacteria, mix and seal and ferment at 20-40° C. for 10-20 days, and filter the fermented liquid to obtain the product.

7. The preparation method according to claim 6, characterized in that: The fermentation time is 15 days.

8. Use of the composition according to any one of claims 1 to 5 in preparing a product capable of simultaneously deodorizing and promoting plant growth.

9. The use according to claim 8, characterized in that: The plant is Camellia oleifera.

Images