Rhododendron mycorrhizal fungi WF17-2-1, application of rhododendron mycorrhizal fungi WF17-2-1 in blueberry growth promotion and product

Abstract

The invention relates to a rhododendron mycorrhizal fungus WF17-2-1, application thereof in blueberry growth promotion and a product thereof, the strain WF17-2-1 can form a mycelium group in epidermal cells of a blueberry root system, significantly improve the growth of the root system, increase the absorption ability of the blueberry to nutrients, promote the synthesis of plant hormones, regulate the growth metabolism of the blueberry and enhance the photosynthesis of the plant, so that the growth of the blueberry is promoted. The strain WF17-2-1 is beneficial to accumulation of nutrient substances such as mineral elements and photosynthetic products of the blueberries, the nutrient value of the blueberries is improved, development of biological fertilizers or growth promoters based on the strain WF17-2-1 is facilitated, and green and efficient development of the blueberry industry is promoted.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, and in particular to an ericoid mycorrhizal fungus WF17-2-1 and its application in promoting the growth of blueberries and products thereof. Background Art

[0002] Blueberry (Vaccinium spp.) is a shrub of the genus Vaccinium in the family Ericaceae. Its fruit is blue, sweet and sour, rich in vitamins, minerals and antioxidants, and has high nutritional value. Therefore, the blueberry planting industry has developed rapidly around the world and has become a product with high economic value. However, the growth of blueberries has high requirements for the soil environment and is difficult to cultivate, resulting in low levels of commercialization and large-scale production of blueberries, which has hindered the development of the blueberry industry. Existing blueberry growth promotion technologies are heavily dependent on the selection of improved varieties and the application of chemical fertilizers. The cycle of breeding improved varieties is long and there are few varieties, while the application of chemical fertilizers is prone to food residues and environmental pollution. Therefore, the development of the blueberry industry requires greener and more efficient technical support.

[0003] Ericoidmycorrhizal fungi (EMF) are a type of soil fungi that can form a symbiosis with plant roots. Their relationship with plants can be divided into three types: mutualism, commensalism, and parasitic symbiosis. Among them, mutualistic fungi can improve the absorption capacity of nutrients and enhance the decomposition and utilization of insoluble complexes and organic matter after being infected by them. While maintaining the needs of the fungi themselves, it also promotes the growth of plants. Therefore, mutualistic strains are often developed as biofertilizers or humus to improve crop growth.

[0004] The screening of mutually beneficial symbiotic fungi is of great significance to the cultivation and development of blueberries. On the one hand, the rational development of symbiotic fungi can alleviate the technical difficulties of blueberry cultivation, improve the quality and yield of blueberries, and promote the diversification of the blueberry industry. On the other hand, the compounding of symbiotic fungi into biofertilizer can reduce the use of chemical fertilizers and help solve problems such as soil acidification and salinization in the process of blueberry cultivation. It is crucial to the sustainable development of the blueberry industry and the protection of the ecological environment. Summary of the invention

[0005] In view of the above problems, the present invention provides a rhododendron mycorrhizal fungus WF17-2-1 and its application. A rhododendron mycorrhizal fungus was screened from the root system of Vaccinium bracteatum Thunb. After infecting the blueberry root system, the strain can improve the blueberry's ability to absorb nutrients and promote the growth of blueberries, which is of great significance for reducing costs and increasing efficiency in the blueberry industry.

[0006] An application of azalea mycorrhizal fungus WF17-2-1, wherein the azalea mycorrhizal fungus WF17-2-1 was deposited in Guangdong Provincial Microbiological Culture Collection Center on August 1, 2024, with a deposit number of GDMCC No.64931.

[0007] Through fungal pure culture morphological identification, the biological characteristics of the strain WF17-2-1 are filamentous fungi, and the colony morphology on the solid culture medium is irregular yellow colonies with a convex middle and filamentous edges.

[0008] Molecular biological identification showed that strain WF17-2-1 belonged to the phylum Ascomycota, class Leotiomycetes, orders Helotiales, Hyaloscyphaceae, Hyaloscypha.sp.

[0009] Preferably, the application of the ericoid mycorrhizal fungus WF17-2-1 in increasing the biomass of blueberries, wherein the strain is co-cultivated with blueberries, can increase the fresh weight and dry weight of the whole plant of blueberries.

[0010] Preferably, the application of the ericoid mycorrhizal fungus WF17-2-1 in improving the root system of blueberries, the strain is co-cultured with blueberries, can form mycelium masses in the epidermal cells of the blueberry roots, and increase the root length, root surface area and volume of the blueberries.

[0011] Preferably, the application of the ericoid mycorrhizal fungus WF17-2-1 in regulating the growth metabolism of blueberries, wherein the strain is co-cultured with blueberries, can increase the accumulation of zeatin riboside in blueberries.

[0012] Preferably, the use of the ericoid mycorrhizal fungus WF17-2-1 in promoting blueberry photosynthesis, wherein the strain is co-cultivated with blueberry, can enhance the photosynthetic enzyme activity of blueberry.

[0013] Preferably, the application of the ericoid mycorrhizal fungus WF17-2-1 in improving the nutritional value of blueberries, wherein the strain is co-cultured with blueberries, can increase the total phosphorus and total potassium content of the blueberries.

[0014] Preferably, the ericoid mycorrhizal fungus WF17-2-1 and its applications include application of strain WF17-2-1 in blueberry cultivation.

[0015] A product capable of promoting the growth of blueberries, comprising ericoid mycorrhizal fungus WF17-2-1 or a metabolite thereof.

[0016] Preferably, the product comprises a growth promoter or a growth promoting fertilizer.

[0017] Compared with the prior art, the beneficial effect of the present invention lies in that the ericoid mycorrhizal fungus WF17-2-1 can symbiotically coexist with the blueberry root system, form mycelium masses in the epidermal cells of the blueberry root system, significantly improve root growth, increase nutrient absorption, promote the synthesis of plant hormones, regulate plant growth metabolism, enhance plant photosynthesis, contribute to the accumulation of nutrients in blueberries, improve the nutritional value of blueberries, and is conducive to the development of bio-fertilizers or growth promoters based on strain WF17-2-1, thereby promoting the green and efficient development of the blueberry cultivation industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and, together with the description, serve to explain the principles of the invention.

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0020] In the attached figure:

[0021] Figure 1 :The hyphae formed by the mycelial mass of the ericoid mycorrhizal fungus WF17-2-1 infecting the epidermal cells of blueberry roots;

[0022] Figure 2 : Colony morphology of ericoid mycorrhizal fungus WF17-2-1;

[0023] Figure 3 :The phylogenetic tree constructed based on the sequencing results of WF17-2-118S rDNA of ericoid mycorrhizal fungi;

[0024] Figure 4 :The phylogenetic tree constructed based on the sequencing results of the ericoid mycorrhizal fungus WF17-2-1ITS;

[0025] Figure 5 :The biomass test results of blueberry inoculated with ericoid mycorrhizal fungus WF17-2-1;

[0026] Figure 6 : Root length test results of blueberry after inoculation with ericoid mycorrhizal fungus WF17-2-1;

[0027] Figure 7 : Root surface area test results of blueberry inoculated with ericoid mycorrhizal fungus WF17-2-1;

[0028] Figure 8 : Root volume test results of blueberry inoculated with ericoid mycorrhizal fungus WF17-2-1;

[0029] Fig. 9 : Zeatin riboside test results after blueberry inoculated with ericoid mycorrhizal fungus WF17-2-1;

[0030] Fig.10 :Total phosphorus test results of blueberry inoculated with ericoid mycorrhizal fungus WF17-2-1;

[0031] Fig.11 :Total potassium test results after blueberry inoculated with ericoid mycorrhizal fungus WF17-2-1;

[0032] Fig.12 :The results of the maximum quantum yield test of photosystem II after blueberry was inoculated with ericoid mycorrhizal fungus WF17-2-1;

[0033] Fig.13 : Actual light quantum efficiency test results of blueberry after inoculation with ericoid mycorrhizal fungus WF17-2-1. DETAILED DESCRIPTION

[0034] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Without departing from the spirit and essence of the present invention, modifications or substitutions made to the methods, steps or conditions of the present invention all belong to the scope of protection of the present invention. Unless otherwise specified, the experimental materials, reagents, instruments, etc. used in the examples of the present invention are all commercially available; unless otherwise specified, all technical means in the examples of the present invention are conventional means well known to those skilled in the art.

[0035] A rhododendron mycorrhizal fungus WF17-2-1 and an application thereof. The rhododendron mycorrhizal fungus WF17-2-1 was deposited in Guangdong Provincial Microbiological Culture Collection Center on August 1, 2024, with a deposit number of GDMCC No.64931.

[0036] Preferably, the ericoid mycorrhizal fungus WF17-2-1 and its applications include application of strain WF17-2-1 in blueberry cultivation.

[0037] Preferably, the application of the ericoid mycorrhizal fungus WF17-2-1 in blueberry cultivation includes any of the following applications:

[0038] The application of ericoid mycorrhizal fungus WF17-2-1 in increasing blueberry biomass, including increasing the total fresh weight and total dry weight of the whole plant;

[0039] The application of the ericoid mycorrhizal fungus WF17-2-1 in improving the root system of blueberries, including the formation of hyphae in the epidermal cells of the blueberry roots, which increased the root length, root surface area and volume of blueberries;

[0040] The application of ericoid mycorrhizal fungus WF17-2-1 in regulating the growth metabolism of blueberry, including the increase of zeatin riboside accumulation in blueberry;

[0041] Application of ericoid mycorrhizal fungus WF17-2-1 in promoting blueberry photosynthesis, including enhancement of blueberry photosynthetic enzyme activity;

[0042] The application of ericaceous mycorrhizal fungus WF17-2-1 in improving the nutritional value of blueberries, including increasing the total phosphorus and total potassium content of blueberries.

[0043] A product capable of promoting the growth of blueberries, comprising ericoid mycorrhizal fungus WF17-2-1 or a metabolite thereof.

[0044] Preferably, the product comprises a growth promoter or a growth promoting fertilizer.

[0045] Example 1 Strain Isolation

[0046] The samples of Psoralea corylifolia were collected in Shangzhen Village, Daba Town, Heping County, Heyuan City, Guangdong Province (coordinates: 24°31'N, 114°55'E; altitude: 207m), and the strains were isolated from the roots of the samples according to the following steps:

[0047] (1) Cleaning: Clean the soil and impurities from the root system of the sample, select young roots with a diameter of 0.5-1.0 mm from the cleaned root system as separation materials, and soak the separation materials in sterile water to remove impurities.

[0048] (2) Disinfection: In a sterile environment, soak the separation material in 75% alcohol for 2 minutes and then rinse with sterile water. This is alcohol disinfection. Repeat the alcohol disinfection step 3 times. Soak the separation material in 5% sodium hypochlorite for 15 minutes and rinse with sterile water.

[0049] (3) Isolation and culture: Under a sterile environment, the isolated material was divided into root segments of 0.3-0.5 cm and transferred to potato dextrose agar (PDA) medium, with five root segments placed in each medium. The medium was placed in a dark environment at 25°C for 2-4 weeks until colonies grew out of the root segments.

[0050] (4) Purification: Under a sterile environment, use a pick to pick a small amount of hyphae from the edge of each colony, transfer it to a new PDA medium, and culture it in the dark at 25°C. Repeat this step until a single colony is obtained.

[0051] Example 2 Strain Screening

[0052] From all the single colonies obtained in Example 1, strains capable of infecting the root system of blueberry (test plant: blueberry tissue culture seedling ZY01) were selected and screened according to the following steps:

[0053] (1) Preparation of bacterial suspension: Mycelium was picked from all the single colonies obtained in Example 1 and transferred to potato dextrose water (PDB) medium. The PDB medium was placed in a shaker at 25° C. and 150 rpm for 2 weeks to obtain single colony bacterial suspension.

[0054] (2) Strain inoculation: Two blueberry tissue culture seedlings were grouped together. Under a sterile environment, 10 mL of a single colony culture solution was inoculated onto the culture medium of the tissue culture seedlings. Five groups were treated with each single colony culture solution. At the same time, five groups of tissue culture seedlings were treated with an equal amount of sterile PDB culture medium as a control. The treated tissue culture seedlings were cultured at 25°C for 20 days.

[0055] (3) Preparation of mycorrhizal sections by trypan blue staining:

[0056] Fixation: After the culture is completed, the blueberry root system is removed, young roots with a diameter of 0.5-1.0 mm are selected, washed, cut into 1 cm root segments, and placed in a freshly prepared standard fixative (38% formaldehyde: acetic acid: glycerol: 50% alcohol = 1:1:1:18) and immersed for 24 hours.

[0057] Acidification: After fixation, take out the root segments, wash them with water, soak them in 2% hydrochloric acid for 5 minutes, and wash them again until clean.

[0058] Staining: Soak the acidified root segments in 0.05% trypan blue staining solution and heat at 90°C for 30 minutes. Take them out and wash them clean, transfer them to decolorizing solution (lactic acid: glycerol = 1:1) and soak them for one day, then take them out and wash them.

[0059] Preparation: Drop a drop of water in the center of the slide, place the stained root segment in glycerol, cover with a coverslip, and obtain blueberry root segment slices.

[0060] Through microscopic observation of the slices, it was found that the strain WF17-2-1 had the best infection ability and could form hyphae in the epidermal cells of the blueberry root system, forming a good mutually beneficial symbiotic relationship with the blueberry root system. Figure 1 shown.

[0061] Example 3 Strain Identification

[0062] Morphological identification: Under a sterile environment, a small amount of marginal hyphae was picked from the purified single colony of strain WF17-2-1 in Example 1, placed on a new PDA medium, cultured in a dark environment at 25° C. for 15 days, and the biological morphology of the colony was observed.

[0063] The identification results showed that the biological characteristics of strain WF17-2-1 were filamentous fungi, and the colony morphology on solid culture medium was irregular yellow colonies with a convex middle and filamentous edges.

[0064] Molecular biological identification: A small amount of single colony culture fluid of strain WF17-2-1 was taken to extract DNA, and the strain ribosome internal transcribed spacer (ITS) and ribosomal small subunit (18S rDNA) were amplified and sequenced respectively. The sequencing primers are as follows:

[0065] ITS sequencing primers:

[0066] ITS1:5ˊ-TCCGTAGGTGAACCTGCGG-3ˊ

[0067] ITS4:5ˊ-TCCTCCGCTTATTGATATGC-3ˊ

[0068] 18S rDNA sequencing primers:

[0069] NS1:5ˊ-GTAGTCATATGCTTGTCTC-3ˊ

[0070] NS8:5ˊ-TCCGCAGGTTCACCTACGGA-3ˊ

[0071] The results of ITS and 18S rDNA sequencing are shown in the sequence listing SEQ ID NO: 1 and SEQ ID NO: 2. The Kimura 2-parameter model of MEGA11 and the NJ algorithm (bootstrap repeated 1000 times) were used to perform phylogenetic tree analysis on the sequenced sequences in combination with the NCBI database. The phylogenetic tree results of the 18SrDNA sequencing results and the ITS sequencing results were finally obtained as shown in Figure 3 , Figure 4 shown.

[0072] According to the results of 18S rDNA phylogenetic tree analysis, strain WF17-2-1 belongs to the order Helotiales, and according to the results of ITS phylogenetic tree analysis, strain WF17-2-1 belongs to the genus Leohumicola.

[0073] Therefore, strain WF17-2-1 was identified as Ascomycota, Leotiomycetes, Helotiales, Hyaloscyphaceae, Hyaloscypha.sp.

[0074] Example 4 Co-cultivation of strains and blueberries

[0075] The strain WF17-2-1 was inoculated into blueberry tissue culture seedlings for co-cultivation. After the co-cultivation, various physiological indicators of blueberry were detected to evaluate the growth-promoting effect of the strain on blueberry.

[0076] Follow the steps below to perform co-culture:

[0077] (1) Preparation of microbial preparation: Under a sterile environment, a small amount of marginal mycelium was picked from the purified single colony of strain WF17-2-1 in Example 1, placed on a new PDA medium, and cultured in a dark environment at 25° C. for 15 days. The resulting single colony was a microbial preparation.

[0078] (2) Inoculation of strains: Under a sterile environment, the rooted blueberry tissue culture seedlings (variety ZY01) were transferred to a culture bottle containing a sterilized matrix and a blueberry-specific culture medium. After hardening, four portions of the bacterial agent were inoculated into the matrix of each tissue culture seedling, and a total of 15 plants were treated as the experimental group. At the same time, 15 new tissue culture seedlings were taken and four portions of PDA culture medium were inoculated into the matrix of each seedling as the control group. The experimental group and the control group were placed in a 25°C incubator and co-cultured for two months.

[0079] (3) Index detection: After the co-cultivation, samples from the experimental group and the control group were collected and tested for various physiological indicators of blueberries.

[0080] Example 5 Blueberry biomass detection

[0081] The blueberry tissue culture seedlings were removed from the culture medium, cleaned of impurities, and weighed after wiping off the surface moisture to obtain the total fresh weight test result; the blueberries were then placed in an oven at 105°C for 15 minutes to kill green, and the temperature was lowered to maintain 80°C for continuous drying until the weight of the tissue culture seedlings no longer changed, and weighed to obtain the total dry weight test result.

[0082] like Figure 5 As shown, compared with the control group (CK), the total fresh weight of blueberries in the experimental group after inoculation with WF17-2-1 inoculant exceeded 0.4 g, an increase of 18.3%, and the total dry weight increased by 16.8%. The total fresh weight and total dry weight are indicators for evaluating plant biomass, indicating that the accumulation of water content and organic matter increased (* indicates p < 0.1, there is a difference between the two), increasing the biomass of the whole plant, and contributing to the increase in blueberry yield.

[0083] Example 6 Detection of blueberry root growth index

[0084] After washing the roots of the tissue culture seedlings, they were scanned using a WINRHIZO root scanner, and relevant indicators of root growth such as root length, root volume, and root surface area were calculated based on the scanned images.

[0085] like Figure 6 , Figure 7 and Figure 8 As shown in the figure, compared with the control group (CK), the root length of the blueberries in the experimental group increased from less than 35 cm to nearly 60 cm after being inoculated with the WF17-2-1 inoculant, and the root surface area increased from less than 3 cm 2 Increased to nearly 5.5cm 2 , the root volume is less than 0.02cm3 Grow to more than 0.04cm 3 , root length, root surface area and root volume increased by 82.3%, 104.4% and 130.9% respectively, indicating that after the strain WF17-2-1 and the blueberry root system coexisted, the cell growth and proliferation rate of the root system were significantly improved (* indicates p < 0.1, there is a difference between the two, ** indicates p < 0.05, there is a significant difference between the two), which accelerated the growth and development of the root system, improved the growth structure of the root system, helped to improve the absorption capacity of the blueberry root system, and promoted the growth of blueberries.

[0086] Example 7 Detection of Zeatin Riboside Content in Blueberries

[0087] The whole plant samples of tissue culture seedlings were taken, washed, and fully ground, and the content of zeatin riboside was detected by double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA).

[0088] like Fig. 9 As shown, compared with the control group (CK), the zeatin riboside (ZR) content of the blueberries in the experimental group after inoculation with the WF17-2-1 inoculant was as high as 4.6 ng / mL, an increase of 18.1% (* indicates p < 0.1, there is a difference between the two). Zeatin riboside, as a plant growth regulating hormone, can promote cell division, stimulate the differentiation of lateral buds and the formation of callus tissue. The accumulation of zeatin riboside can regulate the growth metabolism of blueberries and accelerate growth and development.

[0089] Example 8 Detection of total potassium and total phosphorus content in blueberries

[0090] The total phosphorus content was detected by phosphomolybdenum blue colorimetry, and the total potassium content was detected by sodium tetraphenylborate turbidimetry.

[0091] like Fig.10 and Fig.11 As shown, compared with the control group (CK), the total phosphorus content of the blueberries in the experimental group increased by more than 0.5 g / kg, an increase of 19.5%, and the total potassium content increased by 18.2% after being inoculated with the WF17-2-1 bacterial agent, indicating that after the strain WF17-2-1 and the root system coexisted, the nutrient absorption capacity of the root system was significantly improved, and the phosphorus and potassium accumulation of the whole blueberry plant was increased (** indicates p < 0.05, there is a significant difference between the two, *** indicates p < 0.001, there is an extremely significant difference between the two), thereby improving the nutritional value of blueberries.

[0092] Example 9: Detection of photosynthetic capacity of blueberries

[0093] After washing the leaf samples of tissue culture seedlings, the chlorophyll fluorescence characteristics were measured using the chlorophyll fluorescence instrument IMAGING-PAM chlorophyll fluorescence imaging system to calculate the maximum quantum yield and actual quantum efficiency of PSII.

[0094] like Fig.12 and Fig.13 As shown in the figure, compared with the control group (CK), the blueberries in the experimental group inoculated with WF17-2-1 had a lower PS II maximum quantum yield (Fv / Fm) increased significantly by 1.6% (** indicates p < 0.05, there is a significant difference between the two), and the actual light quantum efficiency (Y(II)) increased from less than 0.23% to nearly 0.33%, a significant increase of 53.8% (*** indicates p < 0.001, there is a very significant difference between the two). The maximum quantum yield refers to the highest light energy conversion efficiency per unit area when the functional leaves of the plant are in the optimal state, representing the limit value of the leaves' utilization of absorbable radiation, while the actual light quantum efficiency refers to the light energy conversion efficiency of the leaves under actual conditions, representing the light energy absorbed by the leaves per unit area per unit time. These chlorophyll fluorescence characteristic parameters can reflect the activity of leaf photosynthetic enzymes. The experimental results fully demonstrate that the light energy conversion efficiency of blueberry leaves is significantly improved when they are in the optimal state. Under the same conditions, the inoculated strain WF17-2-1 can enhance the activity of leaf photosynthetic enzymes, promote blueberry photosynthesis, increase the accumulation of photosynthetic products, and help increase blueberry yield.

[0095] Based on the above experimental results, among all the strains isolated from Wuranzi, strain WF17-2-1 showed better infection ability. Therefore, strain WF17-2-1 was selected as the blueberry root symbiotic bacteria. After the blueberries were inoculated with strain WF17-2-1, the strain could form mycelium in the epidermal cells of the blueberry roots, significantly improving root growth, increasing nutrient absorption, promoting the synthesis of plant hormones, regulating plant growth metabolism, enhancing plant photosynthesis, contributing to the accumulation of nutrients in blueberries, and improving the nutritional value of blueberries. It is also conducive to the development of biofertilizers or growth promoters based on strain WF17-2-1, and promoting the green and efficient development of the blueberry industry.

[0096] It can be understood that the above embodiments only express the preferred implementation modes of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the patent scope of the present invention. It should be pointed out that, for ordinary technicians in this field, the above technical features can be freely combined without departing from the concept of the present invention, and several deformations and improvements can be made, which all belong to the protection scope of the present invention. Therefore, all equivalent changes and modifications made to the scope of the claims of the present invention should belong to the coverage of the claims of the present invention.

Claims

1. A rhododendron mycorrhizal fungus WF17-2-1, characterized in that: The azalea mycorrhizal fungus WF17-2-1 was deposited in the Guangdong Provincial Microbiological Culture Collection Center on August 1, 2024, with the collection number GDMCC No. 64931, and the collection address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou.

2. Use of the ericoid mycorrhizal fungus WF17-2-1 described in claim 1 in increasing blueberry biomass.

3. Use of the ericoid mycorrhizal fungus WF17-2-1 described in claim 1 in improving the root system of blueberries.

4. Use of the ericoid mycorrhizal fungus WF17-2-1 described in claim 1 in regulating the growth metabolism of blueberries.

5. Use of the ericoid mycorrhizal fungus WF17-2-1 described in claim 1 in promoting blueberry photosynthesis.

6. Use of the ericoid mycorrhizal fungus WF17-2-1 described in claim 1 in improving the nutritional value of blueberries.

7. A product capable of promoting the growth of blueberries, characterized in that: Contains the ericoid mycorrhizal fungus WF17-2-1 or its metabolites as described in claim 1.

8. The product according to claim 7, characterized in that The products include growth promoters or growth promoting fertilizers.

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