Low-temperature synergistic compound disinfectant and preparation method thereof

Abstract

The invention provides a low-temperature synergistic compound disinfectant and a preparation method thereof, and relates to the technical field of disinfectants. The low-temperature synergistic composite disinfectant provided by the invention is prepared from the following raw materials in percentage by weight: 15 percent to 20 percent of ethanol, 0.1 percent to 0.2 percent of chlorhexidine acetate, 0.1 percent to 0.2 percent of chlorhexidine gluconate, 0.05 percent to 0.15 percent of benzalkonium chloride, 8 percent to 10 percent of propylene glycol, 0.1 percent to 0.2 percent of EDTA (Ethylene Diamine Tetraacetic Acid)-tetrasodium, 0.5 percent to 1 percent of alkyl glycoside (APG), 0.1 percent to 0.15 percent of sodium silicate, 0.1 percent to 0.15 percent of disodium hydrogen phosphate, 1 percent to 2 percent of ethanolamine, 0.05 percent to 0.15 percent of essence and the balance of deionized water. By adjusting the raw material composition and the preparation method of the disinfectant, the dependence of the disinfectant on ethanol is reduced, the safety of the disinfectant is improved, the transportation and storage limitations are reduced, and the cost and the risk are reduced, so that the requirement of low ethanol concentration in cold regions is met.

Description

Technical Field

[0001] The invention relates to the technical field of disinfectants, and in particular to a low-temperature synergistic composite disinfectant and a preparation method thereof. Background Art

[0002] Disinfectant is a preparation that kills or inhibits pathogenic microorganisms through chemical or biological methods. It is widely used in medical, household, industrial, agricultural and public facilities fields. Its core function is to cut off the transmission route of pathogens and ensure health safety.

[0003] At present, alcohol disinfectants mainly based on ethanol (alcohol) are the most common disinfectants. However, ordinary alcohol-containing disinfectants tend to freeze or become sticky at low temperatures (such as in cold areas, cold storage, and outdoors in winter), making it impossible to spray or apply them normally. In addition, in low-temperature environments such as vaccine transportation and biological sample storage, disinfectants need to be used directly at low temperatures to avoid the risks caused by heating operations. Therefore, it is necessary to develop disinfectants with low-temperature resistance.

[0004] In addition, traditional high-concentration ethanol (60%-80%) disinfectants are flammable and can easily cause accidents in confined spaces or environments with fire sources (such as laboratories and oil depots). Therefore, a low-ethanol content formula can improve the safety of disinfectants. In addition, high-concentration alcohol can easily cause dry skin and allergies, and low-ethanol formulas are more suitable for high-frequency use scenarios to avoid damaging the surface of the material and irritating the skin of the user. At the same time, ethanol is a volatile organic compound (VOC), and a low-ethanol formula can reduce environmental pollution.

[0005] It can be seen that developing a disinfectant that is resistant to low temperatures, has a low ethanol content, has good fluidity and stability under low temperature conditions, and can maintain good disinfection function is of great significance to overcoming the problems existing in existing disinfectants. Summary of the invention

[0006] In view of this, the present invention provides a low-temperature synergistic composite disinfectant with a low ethanol concentration and a preparation method thereof. The present invention reduces the dependence of the disinfectant on ethanol, improves the safety of the disinfectant, and reduces the cost and risk of transportation and storage by adjusting the raw material composition of the disinfectant and its preparation method, so as to meet the demand for low ethanol concentration in cold areas.

[0007] The first aspect of the present invention is to provide a low-temperature synergistic composite disinfectant, comprising the following raw materials:

[0008] Ethanol 15wt.%~20wt.%, chlorhexidine acetate 0.1wt.%~0.2wt.%, chlorhexidine gluconate 0.1wt.%~0.2wt.%, benzalkonium chloride 0.05wt.%~0.15wt.%, propylene glycol 8wt.%~10wt.%, EDTA-tetrasodium 0.1wt.%~0.2wt.%, alkyl glycoside (APG) 0.5wt.%~1wt.%, sodium silicate 0.1wt.%~0.15wt.%, disodium hydrogen phosphate 0.1wt.%~0.15wt.%, ethanolamine 1wt.%~2wt.%, flavor 0.05wt.%~0.15wt.%, and the balance is deionized water.

[0009] Preferably, the mass ratio of the sodium silicate to disodium hydrogen phosphate is 1:1.

[0010] Preferably, the disinfectant comprises the following raw materials by mass percentage:

[0011] Ethanol 20wt.%, chlorhexidine acetate 0.15wt.%, chlorhexidine gluconate 0.15wt.%, benzalkonium chloride 0.1wt.%, propylene glycol 9wt.%, EDTA-tetrasodium 0.15wt.%, APG 0.75wt.%, sodium silicate 0.125wt.%, disodium hydrogen phosphate 0.125wt.%, ethanolamine 1.5wt.%, essence 0.1wt.%, and the balance is deionized water.

[0012] The second aspect of the present invention is to provide a method for preparing a low-temperature synergistic composite disinfectant, which specifically comprises the following steps:

[0013] Dissolve EDTA-tetrasodium in part of deionized water to obtain EDTA-tetrasodium solution; mix ethanol and propylene glycol, stir evenly, add chlorhexidine acetate, chlorhexidine gluconate and benzalkonium chloride, stir until completely dissolved, and obtain a mixed alcohol solution; add sodium silicate and disodium hydrogen phosphate to the EDTA-tetrasodium solution, stir evenly, slowly add the mixed alcohol solution and stir evenly; then slowly add APG, stir until there is no foam, slowly add ethanolamine, add essence, make up with deionized water, homogenize, filter, and obtain a low-temperature synergistic composite disinfectant.

[0014] Preferably, the dissolution temperature of the EDTA-tetrasodium (i.e. the temperature of deionized water) is 40°C to 50°C.

[0015] Preferably, the homogenization speed is 2000-3000 rpm, and the homogenization time is 5-15 min. More preferably, the homogenization speed is 2500 rpm, and the homogenization time is 10 min.

[0016] Compared with the prior art, the beneficial technical effects of the present invention are:

[0017] The disinfectant of the present invention has a lower ethanol concentration, and compared with traditional disinfectants with high ethanol concentrations, the amount of ethanol used is reduced, the potential harm to the environment and human health is reduced, and the transportation and storage costs and risks of the disinfectant are reduced.

[0018] The present invention uses the synergistic effect of the sodium silicate / disodium hydrogen phosphate buffer system and propylene glycol to effectively inhibit low-temperature crystallization and enhance the bactericidal permeability under low-temperature environments; compounding the triple bactericides of chlorhexidine acetate, chlorhexidine gluconate and benzalkonium chloride to achieve broad-spectrum and efficient bactericidal effects through the dual mechanisms of charge adsorption and membrane destruction; combining EDTA-tetrasodium chelating stabilizing components and APG surfactants to optimize detergency, and using the propylene glycol-ethanolamine system to improve low-temperature stability.

[0019] The disinfectant of the present invention has the advantages of low toxicity and safety, strong low-temperature adaptability, and controllable costs. It can be expanded to scenarios that are difficult to cover with traditional high-alcohol disinfectants, such as cold chain logistics, medical low-temperature equipment, and outdoor operations, and has good application prospects. DETAILED DESCRIPTION

[0020] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] The first aspect of the present invention is to provide a low-temperature synergistic composite disinfectant, comprising the following raw materials:

[0022] Ethanol 15wt.%~20wt.%, chlorhexidine acetate 0.1wt.%~0.2wt.%, chlorhexidine gluconate 0.1wt.%~0.2wt.%, benzalkonium chloride 0.05wt.%~0.15wt.%, propylene glycol 8wt.%~10wt.%, EDTA-tetrasodium 0.1wt.%~0.2wt.%, APG 0.5wt.%~1wt.%, sodium silicate 0.1wt.%~0.15wt.%, disodium hydrogen phosphate 0.1wt.%~0.15wt.%, ethanolamine 1wt.%~2wt.%, essence 0.05wt.%~0.15wt.%, and the balance is deionized water; the mass ratio of the sodium silicate to the disodium hydrogen phosphate is 1:1.

[0023] In some specific embodiments of the present invention, the low-temperature synergistic composite disinfectant comprises the following raw materials:

[0024] Ethanol 15wt.%, chlorhexidine acetate 0.1wt.%, chlorhexidine gluconate 0.1wt.%, benzalkonium chloride 0.05wt.%, propylene glycol 8wt.%, EDTA-tetrasodium 0.1wt.%, APG 0.5wt.%, sodium silicate 0.1wt.%, disodium hydrogen phosphate 0.1wt.%, ethanolamine 1wt.%, essence 0.05wt.%, and the balance is deionized water.

[0025] In some specific embodiments of the present invention, the low-temperature synergistic composite disinfectant comprises the following raw materials:

[0026] Ethanol 20wt.%, chlorhexidine acetate 0.2wt.%, chlorhexidine gluconate 0.2wt.%, benzalkonium chloride 0.15wt.%, propylene glycol 10wt.%, EDTA-tetrasodium 0.2wt.%, APG 1wt.%, sodium silicate 0.15wt.%, disodium hydrogen phosphate 0.15wt.%, ethanolamine 2wt.%, essence 0.15wt.%, and the balance is deionized water.

[0027] In some preferred embodiments of the present invention, the low-temperature synergistic composite disinfectant comprises the following raw materials by mass percentage:

[0028] Ethanol 20wt.%, chlorhexidine acetate 0.15wt.%, chlorhexidine gluconate 0.15wt.%, benzalkonium chloride 0.1wt.%, propylene glycol 9wt.%, EDTA-tetrasodium 0.15wt.%, APG 0.75wt.%, sodium silicate 0.125wt.%, disodium hydrogen phosphate 0.125wt.%, ethanolamine 1.5wt.%, essence 0.1wt.%, and the balance is deionized water.

[0029] In disinfectant of the present invention, there are three kinds of cationic surfactants of chlorhexidine acetate, chlorhexidine gluconate and benzalkonium chloride simultaneously.Three are positively charged quaternary ammonium salt compounds, and using together is prone to charge repulsion or intermolecular aggregation, causing product turbidity or even precipitation, especially under low temperature conditions, this phenomenon is more obvious, causing product stability to reduce, and bactericidal effect is also significantly affected.For this reason, the present invention has introduced alkyl glycoside, EDTA-tetrasodium, ethanolamine in system and suitably adjusts the ratio and the order of addition of each component, to avoid the generation of problems such as product turbidity or even precipitation.

[0030] In addition, the superposition of sodium silicate and ethanolamine can cause the hydrolysis failure of chlorhexidine (chlorhexidine acetate, chlorhexidine gluconate), and Hofmann degradation of benzalkonium chloride. At the same time, the irritation of the disinfectant is inevitably increased. Therefore, the present invention replaces part of sodium silicate with disodium hydrogen phosphate to stabilize the pH of the product.

[0031] Propylene glycol lowers the freezing point of the solution and increases the solubility and dispersibility of each component at low temperatures.

[0032] The multiple active ingredients in the disinfectant of the present invention form a synergistic disinfection system. The optimized design of the multi-component synergistic sterilization and low-temperature stabilization system of the present invention enables the disinfectant of the present invention to achieve excellent disinfection effect under the premise of low ethanol content.

[0033] First, the disinfectant of the present invention contains a cationic surfactant triple combination system composed of chlorhexidine acetate, chlorhexidine gluconate and benzalkonium chloride. The cationic surfactant and ethanol work together to destroy the membrane structure, enhance the penetration of ethanol into the cell contents, and the cationic component remains on the surface to continuously inhibit the regeneration of microorganisms. At the same time, the three complement each other in targeting different strains (such as gram-positive bacteria, gram-negative bacteria, and fungi), reduce the risk of drug resistance of a single component, and broaden the antibacterial spectrum.

[0034] Non-ionic APG reduces surface tension and promotes the penetration of ingredients into microorganisms. Propylene glycol, as a polar solvent, avoids low-temperature crystallization and increases the solubility of cationic surfactants to prevent precipitation. It can also delay the volatilization of ethanol and maintain the contact time between active ingredients and microorganisms in low-temperature environments.

[0035] Sodium silicate / disodium hydrogen phosphate form a buffer system to ensure that the cationic surfactant is in the best ionization state and avoid excessive hydrolysis of ethanolamine. Silicate forms a three-dimensional network structure to physically block the aggregation of cationic molecules; phosphate reduces charge repulsion through electrostatic shielding, and the dual mechanism prevents turbidity at low temperatures. EDTA-tetrasodium chelates Ca in water 2+ Mg 2+ Plasma, to prevent it from forming insoluble precipitation with cationic surfactants, especially in hard water or low temperature storage conditions is very important.

[0036] The present invention achieves good stability and bactericidal performance in a low-temperature environment through the synergy of multiple bactericidal ingredients and a low-temperature stabilization system, thereby providing an effective disinfection solution for disinfectant application scenarios such as cold regions and cold chain transportation.

[0037] Finally, the disinfectant of the present invention is generally used in household bathroom facilities (such as toilet covers, sinks), public high-frequency contact surfaces (door handles, elevator buttons) and cold chain environments. Therefore, the components of the disinfectant of the present invention must not only take into account the disinfection effect and low-temperature stability, but also need to have compatibility protection for common materials such as metals (stainless steel, aluminum alloys) and ceramics.

[0038] The sodium silicate / disodium hydrogen phosphate system avoids strong alkalinity from oxidative corrosion of metals. 3-A protective layer is formed on the metal surface to inhibit electrochemical corrosion. The combination of ethanol and propylene glycol prevents acidic or strong oxidizing components from corroding the ceramic glaze. APG and silicate colloid work synergistically to prevent scale or crystallization from forming on the ceramic surface after the disinfectant dries. This makes the disinfectant of the present invention safe for use in the disinfection of sanitary facilities, public contact surfaces, and cold chain equipment to meet the needs of long-term high-frequency disinfection and strict requirements for material protection.

[0039] The second aspect of the present invention is to provide a method for preparing a low-temperature synergistic composite disinfectant, which specifically comprises the following steps:

[0040] S1. Accurately weigh each component in proportion, dissolve EDTA-tetrasodium in part of deionized water to obtain EDTA-tetrasodium solution;

[0041] S2, ethanol and propylene glycol are mixed and stirred evenly, and then chlorhexidine acetate, chlorhexidine gluconate and benzalkonium chloride are added to the alcohol mixture, and stirred until completely dissolved to obtain a mixed alcohol solution;

[0042] S3, adding sodium silicate and disodium hydrogen phosphate to the EDTA solution, stirring evenly, then slowly adding the mixed alcohol solution and stirring evenly;

[0043] S4. Slowly add APG and stir until there is no foam, then slowly add ethanolamine. After the system cools to below 20°C, add essence, add deionized water, homogenize, filter, and fill to obtain a low-temperature enhanced composite disinfectant.

[0044] In some specific embodiments of the present invention, the temperature of the deionized water in S1 is 40° C. to 50° C., and the amount of deionized water is 60% of the total amount.

[0045] In some specific embodiments of the present invention, the S2 can be used to properly heat the system to accelerate dissolution, and the heating temperature is 30°C to 35°C.

[0046] In some specific embodiments of the present invention, the homogenization speed in S4 is 2000-3000 rpm, preferably 2500 rpm, and the homogenization time is 5-15 min, preferably 10 min.

[0047] The present invention ensures the stability and functionality of the multi-component system through a preparation process of staged dissolution, precise temperature control and sequential mixing.

[0048] Predissolving EDTA-tetrasodium can quickly form a homogeneous solution to avoid subsequent precipitation caused by undissolved particles. Using 60% deionized water as the initial solvent provides sufficient dilution space for subsequent alcohol solution mixing to prevent local excessive concentration. Moderate heating of the alcohol mixture (30℃~35℃) can reduce the viscosity of the mixed alcohol, accelerate the dissolution of the cationic surfactant, and avoid excessive temperature causing ethanol volatilization or thermal decomposition of chlorhexidine.

[0049] Dissolution in stages can avoid EDTA and cationic surfactants from coming into contact too early to produce complexation reactions. At the same time, temperature graded control matches the physicochemical properties of each component to avoid affecting the effect of the disinfectant. The 1:1 buffer system of sodium silicate and disodium hydrogen phosphate is formed before the addition of alcohol to avoid ethanol interfering with silicate dissolution. APG is prone to foaming. Slow addition and low-speed stirring can reduce bubble generation and avoid foam wrapping undispersed particles and affecting homogeneity.

[0050] Fragrances are mostly volatile organic compounds. Low-temperature addition reduces volatilization losses and avoids decomposition of aroma components caused by high temperatures. High-speed homogenization can ensure nano-level dispersion and improve the low-temperature storage stability of disinfectants. Insufficient homogenization will lead to uneven distribution of ingredients, which are prone to stratification or precipitation at low temperatures; excessive homogenization may destroy the colloidal structure, and the speed and time need to be optimized through experiments. Therefore, the present invention limits the speed and time of homogenization to balance the homogenization effect.

[0051] In order to further illustrate the present invention, the following examples are provided for detailed description. The raw materials used in the following examples of the present invention are all commercially available.

[0052] Unless otherwise specified, all experiments were repeated three times. SPSS 21.0 was used for analysis of variance (ANOVA) and Duncan's multiple comparison analysis. The results were expressed as mean ± standard deviation, and P < 0.05 indicated a significant difference.

[0053] Embodiment 1 A low-temperature synergistic composite disinfectant, in terms of mass percentage, is composed as follows:

[0054] Ethanol 15wt.%, chlorhexidine acetate 0.1wt.%, chlorhexidine gluconate 0.1wt.%, benzalkonium chloride 0.05wt.%, propylene glycol 8wt.%, EDTA-tetrasodium 0.1wt.%, APG 0.5wt.%, sodium silicate 0.1wt.%, disodium hydrogen phosphate 0.1wt.%, ethanolamine 1wt.%, lemon essence 0.05wt.%, and the balance is deionized water.

[0055] The preparation method of the low-temperature synergistic composite disinfectant solution has the following steps:

[0056] S1. Accurately weigh each component in proportion, dissolve EDTA-tetrasodium in deionized water at a temperature of 40° C., which accounts for 60% of the total amount, to obtain EDTA-tetrasodium solution;

[0057] S2, ethanol and propylene glycol are mixed and stirred evenly, and then chlorhexidine acetate, chlorhexidine gluconate, and benzalkonium chloride are added to the alcohol mixture, and stirred at 30° C. until they are completely dissolved to obtain a mixed alcohol solution;

[0058] S3, adding sodium silicate and disodium hydrogen phosphate to the EDTA solution, stirring evenly, then slowly adding the mixed alcohol solution and stirring evenly;

[0059] S4. Slowly add APG and stir until there is no foam, then slowly add ethanolamine. After the system is cooled to below 20°C, add essence, add deionized water, homogenize at 2000rpm for 5min, filter through 0.22μm filter membrane, and fill to obtain low-temperature enhanced composite disinfectant.

[0060] Embodiment 2 A low-temperature synergistic composite disinfectant, in terms of mass percentage, is composed as follows:

[0061] Ethanol 20wt.%, chlorhexidine acetate 0.2wt.%, chlorhexidine gluconate 0.2wt.%, benzalkonium chloride 0.15wt.%, propylene glycol 10wt.%, EDTA-tetrasodium 0.2wt.%, APG 1wt.%, sodium silicate 0.15wt.%, disodium hydrogen phosphate 0.15wt.%, ethanolamine 2wt.%, lemon essence 0.15wt.%, and the balance is deionized water.

[0062] The preparation method of the low-temperature synergistic composite disinfectant solution has the following steps:

[0063] S1. Accurately weigh each component in proportion, dissolve EDTA-tetrasodium in deionized water at a temperature of 50° C. accounting for 60% of the total amount, to obtain EDTA-tetrasodium solution;

[0064] S2, ethanol and propylene glycol are mixed and stirred evenly, and then chlorhexidine acetate, chlorhexidine gluconate, and benzalkonium chloride are added to the alcohol mixture, and stirred at 35° C. until they are completely dissolved to obtain a mixed alcohol solution;

[0065] S3, adding sodium silicate and disodium hydrogen phosphate to the EDTA solution, stirring evenly, then slowly adding the mixed alcohol solution and stirring evenly;

[0066] S4. Slowly add APG and stir until there is no foam, then slowly add ethanolamine. After the system cools to below 20°C, add essence, add deionized water, homogenize at 3000rpm for 15min, filter with 0.22μm filter membrane, and fill to obtain low-temperature synergistic composite disinfectant.

[0067] Embodiment 3 A low-temperature synergistic composite disinfectant, in terms of mass percentage, is composed as follows:

[0068] Ethanol 20wt.%, chlorhexidine acetate 0.15wt.%, chlorhexidine gluconate 0.15wt.%, benzalkonium chloride 0.1wt.%, propylene glycol 9wt.%, EDTA-tetrasodium 0.15wt.%, APG 0.75wt.%, sodium silicate 0.125wt.%, disodium hydrogen phosphate 0.125wt.%, ethanolamine 1.5wt.%, lemon essence 0.1wt.%, and the balance is deionized water.

[0069] The preparation method of the low-temperature synergistic composite disinfectant solution has the following steps:

[0070] S1. Accurately weigh each component in proportion, dissolve EDTA-tetrasodium in deionized water at a temperature of 450° C., which accounts for 60% of the total amount, to obtain EDTA-tetrasodium solution;

[0071] S2, ethanol and propylene glycol are mixed and stirred evenly, and then chlorhexidine acetate, chlorhexidine gluconate, and benzalkonium chloride are added to the alcohol mixture, and stirred at 35° C. until they are completely dissolved to obtain a mixed alcohol solution;

[0072] S3, adding sodium silicate and disodium hydrogen phosphate to the EDTA solution, stirring evenly, then slowly adding the mixed alcohol solution and stirring evenly;

[0073] S4. Slowly add APG and stir until there is no foam, then slowly add ethanolamine. After the system cools to below 20°C, add essence, add deionized water, homogenize at 2500rpm for 10min, filter with 0.22μm filter membrane, and fill to obtain low-temperature synergistic composite disinfectant.

[0074] Comparative Example 1

[0075] Same as Example 3, except that chlorhexidine acetate was not added.

[0076] Comparative Example 2

[0077] Same as Example 3, except that: chlorhexidine gluconate was not added.

[0078] Comparative Example 3

[0079] Same as Example 3, except that benzalkonium chloride was not added.

[0080] Comparative Example 4

[0081] Same as Example 3, except that no APG was added.

[0082] Comparative Example 5

[0083] Same as Example 3, except that disodium hydrogen phosphate is replaced by an equal amount of sodium silicate.

[0084] Comparative Example 6

[0085] Same as Example 3, except that no ethanolamine was added.

[0086] Comparative Example 7

[0087] The same as Example 3, except that the disinfectant is prepared by the following method:

[0088] Ethanol, chlorhexidine acetate, chlorhexidine gluconate, benzalkonium chloride, propylene glycol, tetrasodium EDTA, APG, sodium silicate, disodium hydrogen phosphate, ethanolamine, essence and deionized water were uniformly mixed in proportion, homogenized at 2500 rpm for 10 min, filtered through a 0.22 μm filter membrane and filled to obtain a low-temperature synergistic composite disinfectant.

[0089] Test Example 1

[0090] Randomly select metal plates of the same specifications at 4°C, with a calibrated area of ​​25 cm 2 There are 110 blocks in the area, which are randomly divided into 10 blocks / group, corresponding to the disinfectants of Examples 1-3 and Comparative Examples 1-7, respectively. The examples are recorded as IE and the comparative examples are recorded as CE. The surface of the metal plate is wiped and disinfected with a 4°C disinfectant. After disinfection, a sample is collected with sterile cotton. After sampling, the sampling end of the cotton swab is folded into a diluent test tube with aseptic operation and shaken. Take 1 mL from each group of samples and inoculate it in a sterile plate. After culturing at 37°C for 48 hours, count the colonies. Perform the same operation with a diluent instead of a disinfectant as a positive control, recorded as PT.

[0091] Among them, the killing logarithm (KL) = the logarithm of the average live bacteria concentration in the control group (N0) - the logarithm of the live bacteria concentration in the test group (Nx)

[0092] The test results are shown in Table 1.

[0093] Table 1

[0094]

[0095]

[0096] * indicates P<0.05 compared with Example 3, ** indicates P<0.01 compared with Example 3.

[0097] Test Example 2

[0098] The low temperature observation method is used to test the stability of the disinfectant under low temperature conditions. The method is as follows: place the disinfectant mixture at -18℃ for 12 hours to observe whether it freezes, has obvious stratification or precipitation. If it remains in a liquid state and has no precipitation or crystallization, it is judged to be qualified. The test results are shown in Table 2.

[0099] project stability IE1 qualified IE2 qualified IE3 qualified CE1 qualified CE2 qualified CE3 qualified CE4 The components are separated out and a small amount of crystals are produced. CE5 The components are separated out and a small amount of crystals are produced. CE6 Components are separated out, and trace crystals are produced CE7 Components are separated out, and more crystals are produced

[0100] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A low-temperature synergistic composite disinfectant, characterized in that: Including the following ingredients: Ethanol 15wt.%~20wt.%, chlorhexidine acetate 0.1wt.%~0.2wt.%, chlorhexidine gluconate 0.1wt.%~0.2wt.%, benzalkonium chloride 0.05wt.%~0.15wt.%, propylene glycol 8wt.%~10wt.%, EDTA-tetrasodium 0.1wt.%~0.2wt.%, alkyl glycoside 0.5wt.%~1wt.%, sodium silicate 0.1wt.%~0.15wt.%, disodium hydrogen phosphate 0.1wt.%~0.15wt.%, ethanolamine 1wt.%~2wt.%, flavor 0.05wt.%~0.15wt.%, and the balance is deionized water.

2. A low-temperature synergistic composite disinfectant according to claim 1, characterized in that: The mass ratio of the sodium silicate to disodium hydrogen phosphate is 1:

1.

3. A low-temperature synergistic composite disinfectant according to claim 1, characterized in that: Including the following ingredients: Ethanol 15wt.%, chlorhexidine acetate 0.1wt.%, chlorhexidine gluconate 0.1wt.%, benzalkonium chloride 0.05wt.%, propylene glycol 8wt.%, EDTA-tetrasodium 0.1wt.%, APG 0.5wt.%, sodium silicate 0.1wt.%, disodium hydrogen phosphate 0.1wt.%, ethanolamine 1wt.%, essence 0.05wt.%, and the balance is deionized water.

4. A low-temperature synergistic composite disinfectant according to claim 1, characterized in that: Including the following ingredients: Ethanol 20wt.%, chlorhexidine acetate 0.2wt.%, chlorhexidine gluconate 0.2wt.%, benzalkonium chloride 0.15wt.%, propylene glycol 10wt.%, EDTA-tetrasodium 0.2wt.%, APG 1wt.%, sodium silicate 0.15wt.%, disodium hydrogen phosphate 0.15wt.%, ethanolamine 2wt.%, essence 0.15wt.%, and the balance is deionized water.

5. The method for preparing the low-temperature synergistic composite disinfectant according to any one of claims 1 to 4, characterized in that: The following steps are involved: Dissolve EDTA-tetrasodium in part of deionized water to obtain EDTA-tetrasodium solution; mix ethanol and propylene glycol and stir evenly, then add chlorhexidine acetate, chlorhexidine gluconate and benzalkonium chloride, stir until completely dissolved to obtain a mixed alcohol solution; add sodium silicate and disodium hydrogen phosphate to the EDTA-tetrasodium solution, stir evenly, then slowly add the mixed alcohol solution and stir evenly; then slowly add APG and stir until there is no foam, then slowly add ethanolamine, add essence, make up with deionized water, homogenize, filter, and obtain a low-temperature synergistic composite disinfectant.

6. The preparation method according to claim 5, characterized in that: The dissolution temperature of the EDTA-tetrasodium is 40°C to 50°C.

7. The preparation method according to claim 5, characterized in that: The homogenizing speed is 2000-3000 rpm, and the homogenizing time is 5-15 minutes.

8. The preparation method according to claim 7, characterized in that: The homogenizing speed is 2500 rpm and the homogenizing time is 10 min.

9. The preparation method according to claim 5, characterized in that: The filtration is performed using a 0.22 μm filter membrane.

10. The preparation method according to claim 5, characterized in that: The filtration process also includes a filling process.

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