Capsule containing Omega3 for improving memory and preparation method thereof

Abstract

The invention discloses an Omega3-containing capsule for improving memory and a preparation method of the Omega3-containing capsule, and belongs to the field of health-care food. The capsule comprises the following components: a composition containing Omega3, polyglycerol polyricinoleate, tocopheryl acetate and purified water, wherein the composition containing Omega3 comprises Omega3, a locust bean extract, a sophora flower extract and a hovenia acerba extract. According to the Omega3-containing composition provided by the invention, all the raw materials are compounded for use, so that a remarkable effect of improving memory is achieved.

Description

Technical Field

[0001] The invention relates to the field of health food, and in particular to a capsule containing Omega 3 for improving memory and a preparation method thereof. Background Art

[0002] With the accelerated pace of modern society and the increasing aging of the population, memory loss has become a common concern. Memory, as an important part of human cognitive function, is crucial for individuals' daily life, study and work. However, there are many reasons for memory loss, including aging, bad living habits, psychological stress, brain and systemic diseases, etc. These factors not only affect the quality of life of individuals, but may also indicate more serious cognitive impairment, such as neurodegenerative diseases such as Alzheimer's disease.

[0003] The need to improve memory is self-evident. Good memory is key to maintaining individual independence and social participation, and is also an important measure to prevent cognitive decline and dementia. In addition, maintaining memory is also crucial to improving work efficiency and academic achievement.

[0004] In the exploration of methods to improve memory, animal / plant extract compositions show great potential and value. Traditional Chinese medicine believes that memory loss is related to heart-kidney disharmony and brain marrow malnutrition, and animals / plants can effectively improve memory through their abundant natural active products such as polypeptides, fatty acids, flavonoids, terpenes, polyphenols, etc. Modern pharmacological studies have also confirmed that some active ingredients in animal / plant extracts such as fatty acids, flavonoids, phenols, alkaloids, etc. can improve learning and memory abilities by increasing the activity of antioxidant enzymes in brain tissue and reducing lipid peroxidation products. These animal / plant compositions not only have neuroprotective effects, but may also play a role by regulating neurotransmitters and increasing cerebral blood flow.

[0005] Based on this, it is urgent to develop a composition comprising animal / plant extracts that can improve memory for consumers to choose. Summary of the invention

[0006] The purpose of the present invention is to provide a capsule containing Omega3 for improving memory and a preparation method thereof, wherein the capsule containing Omega3 for improving memory comprises a composition containing Omega3, the composition is compounded with natural active ingredients and has a significant effect of improving memory.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a composition containing Omega 3, comprising the following ingredients: Omega 3, locust bean extract, locust flower extract, and jujube extract.

[0009] Preferably, the composition containing Omega 3 comprises the following raw materials in parts by weight:

[0010] Omega 3: 4-6 servings;

[0011] Sophora bean extract: 10-13 parts;

[0012] Sophora japonica extract: 5-7 parts;

[0013] Ziziphus jujube extract: 20-25 parts.

[0014] Most preferably, the composition containing Omega 3 comprises the following raw materials in parts by weight:

[0015] Omega3: 5 parts;

[0016] Sophora bean extract: 12 parts;

[0017] Sophora japonica extract: 6 parts;

[0018] Ziziphus jujube extract: 23 parts.

[0019] In a second aspect, the present invention provides a use of the composition containing Omega 3 described in the first aspect in preparing a health product.

[0020] Preferably, the health product is a health product for improving memory containing Omega 3.

[0021] Preferably, the dosage form of the health care product is any one of capsules, tablets, and granules.

[0022] In a third aspect, the present invention provides a capsule containing Omega 3 for improving memory, wherein the capsule comprises capsule contents and a capsule shell, and the capsule contents include the following components in mass percentage:

[0023] 80-90wt% of the composition containing Omega 3 described in the first aspect;

[0024] Polyglycerol polyricinoleate 4-5wt%;

[0025] Tocopherol acetate 1-3wt%;

[0026] Complete with drinking water.

[0027] Preferably, the capsule shell is one of a soft capsule shell and a hard capsule shell.

[0028] More preferably, the capsule shell is a soft capsule shell.

[0029] In a fourth aspect, the present invention provides a method for preparing the capsule according to the third aspect, the method comprising the following steps:

[0030] S1: Evenly mix the Sophora bean extract, Sophora japonica flower extract, and Ziziphus jujuba extract with drinking water to obtain a mixed solution A;

[0031] S2: Ultrasonic mixing of Omega 3, polyglycerol ricinoleate and tocopherol acetate to obtain a mixed solution B;

[0032] S3: Mixing the mixed solution A and the mixed solution B, and performing ultrasonic vibration to make them uniform, to obtain the contents of the capsule;

[0033] S4: Place the capsule contents in a capsule shell to prepare a capsule preparation, sterilize, seal, and store at room temperature away from light.

[0034] Preferably, the capsule preparation in step S4 is a soft capsule preparation.

[0035] Compared with the prior art, the present invention has the following beneficial effects: the present invention provides a capsule containing Omega3 for improving memory and a preparation method thereof, the capsule comprising the following components: a composition containing Omega3, polyglycerol polyricinoleate, tocopherol acetate, and purified water, wherein the composition containing Omega3 is compounded by Omega3, locust bean extract, locust flower extract, and jujube extract, wherein the locust flower extract is rich in flavonoids and has significant antioxidant capacity; Omega3 fatty acids, especially DHA and EPA, also have strong antioxidant properties, can reduce the damage of oxidative stress to the brain, enhance the antioxidant defense system of the brain, and protect nerve cells; the active ingredients in the locust flower extract and the jujube extract can also enhance cognitive function by regulating neurotransmitters and protecting nerve cells, thereby improving memory; the locust bean extract, the locust flower extract, and the jujube extract have strong anti-inflammatory activity, can help reduce neuroinflammation, and improve memory; Omega3 fatty acids together with the alkaloids and flavonoids contained in the locust bean and the locust flower can effectively improve nerve cell function and signal transmission, and further enhance nerve metabolism and cognitive function. Therefore, the combined use of Omega3, Sophora japonica flower extract, Sophora japonica bean extract, and Ziziphus jujuba extract can work together through multiple mechanisms such as anti-oxidation, neuroprotection, anti-inflammation, and improvement of neurometabolism, thereby playing a synergistic role in improving memory. DETAILED DESCRIPTION

[0036] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples, wherein the terms used in the examples are for describing specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0037] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or under conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0038] Some raw materials and their sources are as follows:

[0039] Omega3: purchased from Cargill Grain and Oil (Nantong) Co., Ltd.

[0040] Sophora bean extract: purchased from Shaanxi Xinyanghe Biotechnology Co., Ltd., product number: XYHHJ-001.

[0041] Sophora japonica flower extract: purchased from Yuanpeptide Biotechnology (Tianjin) Co., Ltd.

[0042] Ziziphus jujuba extract: purchased from Xi'an Beijinuo Biotechnology Co., Ltd., product number: BJN89793.

[0043] Polyglycerol polyricinoleate: purchased from Hefei Tianjian Chemical Co., Ltd., CAS: 29894-35-7.

[0044] Tocopherol acetate: purchased from Jiangsu Plus Biotechnology Co., Ltd.

[0045] The raw materials and mass fractions of a composition containing Omega 3 are specifically as follows:

[0046] Composition 1: Omega 3: 5 parts, Sophora japonica extract: 12 parts, Sophora japonica flower extract: 6 parts, Ziziphus jujuba extract: 23 parts.

[0047] Composition 2: Omega 3: 4 parts, Sophora japonica extract: 10 parts, Sophora japonica flower extract: 5 parts, Ziziphus jujuba extract: 20 parts.

[0048] Composition 3: Omega 3: 6 parts, Sophora japonica extract: 13 parts, Sophora japonica flower extract: 7 parts, Ziziphus jujuba extract: 25 parts.

[0049] Composition ①: The difference from composition 1 is that the locust bean extract is missing, and its reduced mass fraction is distributed to other raw materials in proportion.

[0050] Composition ②: The difference from composition 1 is that the Sophora japonica flower extract is missing, and its reduced mass fraction is distributed to other raw materials in proportion.

[0051] Composition ③: The difference from composition 1 is that it lacks the Ziziphus jujuba extract, and its reduced mass fraction is distributed to other raw materials in proportion.

[0052] Composition ④: The difference from composition 1 is that Omega 3 is missing, and its reduced mass fraction is distributed to other raw materials in proportion.

[0053] Composition ⑤: Omega 3: 12 parts, Sophora japonica extract: 5 parts, Sophora japonica flower extract: 23 parts, Ziziphus jujuba extract: 6 parts.

[0054] A capsule containing Omega 3 for improving memory, comprising a gelatin soft capsule shell and capsule contents, wherein the mass percentages of the components in the capsule contents are as follows:

[0055] Capsule content 1: 85wt% of a composition containing Omega 3 (composition 1); 5wt% of polyglycerol polyricinoleate; 2wt% of tocopherol acetate, supplemented with drinking water.

[0056] Capsule content 2: Composition containing Omega 3 90wt% (composition 1); polyglycerol polyricinoleate 4wt%; tocopherol acetate 1wt%, supplemented with drinking water.

[0057] Capsule content 3: Composition containing Omega 3 80wt% (composition 1); polyglycerol polyricinoleate 4wt%; tocopherol acetate 3wt%, supplemented with drinking water.

[0058] Capsule content ①: The difference from capsule content 1 is that glyceryl monostearate is used instead of polyglyceryl ricinoleate.

[0059] The preparation method of capsule 1 is as follows:

[0060] S1: Evenly mix the Sophora bean extract, Sophora japonica flower extract, and Ziziphus jujuba extract with drinking water to obtain a mixed solution A;

[0061] S2: Ultrasonic mixing of Omega 3, polyglycerol ricinoleate and tocopherol acetate to obtain a mixed solution B, wherein the ultrasonic frequency is 40 kHz, the ultrasonic temperature is room temperature, and the ultrasonic time is 10 min;

[0062] S3: Mixing the mixed solution A and the mixed solution B, and performing ultrasonic oscillation to obtain capsule content 1, wherein the ultrasonic frequency is 40 kHz, the ultrasonic temperature is room temperature, and the ultrasonic time is 10 min;

[0063] S4: placing capsule content 1 in a gelatin soft capsule shell to prepare a soft capsule preparation, sterilizing, sealing, and storing at room temperature away from light.

[0064] The preparation method of capsule 2 is different from that of capsule 1 in that capsule content 1 is replaced by capsule content 2, and the remaining steps are the same.

[0065] The preparation method of capsule 3 is different from that of capsule 1 in that capsule content 1 is replaced by capsule content 3, and the remaining steps are the same.

[0066] Preparation method of capsule ①:

[0067] The preparation method is the same as that of capsule 1. The difference from capsule 1 is that capsule content 1 is replaced by capsule content ①, and the remaining steps are the same.

[0068] Memory improvement test

[0069] Experimental animals: SPF SD rats, 8 weeks old, half male and half female, adaptively raised for 1 week.

[0070] Animal modeling: Cognitive dysfunction model: A cognitive dysfunction model was established by chronic scopolamine (Scop) treatment (Deng, G., Wu, C., Rong, X., Li, S., Ju, Z., Wang, Y., Ma, C., Ding, W., Guan, H., Chen, X., Liu, W., Wang, C., 2019. Ameliorative effect of deoxyvasicine on scopolamine-induced cognitive dysfunction by restoration of cholinergic function in mice. Phytomedicine 63, 153007.). In the present invention, except for the normal control group, all other groups received chronic Scop modeling. Scop (CAS number: 51-34-3; MedChemExpress) was intraperitoneally injected at a dose of 1 mg / kg every day for 1 week to establish cognitive dysfunction in mice.

[0071] Experimental animal grouping: Rats were randomly divided into 8 experimental groups, 1 model control group, and 1 normal control group. The experimental group and the model control group were model rats, and the normal control group was normal rats, with 9 rats in each group. The normal control group and the model control group were gavaged with the same volume of normal saline, and the experimental group was gavaged with 0.8g / kg of the corresponding sample, and the drug was administered by gavage for 14 consecutive days, once a day.

[0072] Drug concentration: Compositions 1-3 and compositions ①-⑤ were prepared into 85wt% aqueous solution using drinking water, and then administered orally; capsules 1-3 and capsule ① were administered orally without dilution; the mass of the aqueous solution of compositions 1-3 and compositions ①-⑤ was used as the sample mass, and the mass of the contents of capsules 1-3 and capsule ① was used as the sample mass.

[0073] Water maze setting: The Morris water maze has a diameter of 1.65m, and the inner wall and bottom are completely black. The water maze is divided into four quadrants. A black platform with a diameter of 12cm is placed 2cm below the water surface in the center of the second quadrant. After each water change in the water maze test, a certain amount of black dye is added to make the water black and opaque. The water temperature is maintained at 20±2℃ during the test.

[0074] Experimental training: From the 2nd to the 14th day after drug administration, a positioning navigation test was conducted. During the positioning navigation test, each rat was trained 4 times a day with an interval of 10 minutes. During training, a water entry point was randomly selected and the rat was placed in the water facing the pool wall. The rats entered the water from 4 different water entry points for 4 training sessions. The time required for each rat to enter the water from four different points and find the underwater platform was recorded. If the rat could not find the platform within 120 seconds, the experimenter would take it to the platform to rest for 15 seconds before the next test. The training was conducted 4 times a day with an interval of 120 minutes between each training.

[0075] Space Exploration Experiments:

[0076] Experimental method: On the 15th day, the rats were placed into the water from three randomly selected water entry points. Timing started from each entry into the water and ended when the rats found the original platform. The latency period was the average of the time taken to find the original platform three times.

[0077] During the water maze test, reference objects such as lights around the water maze remained unchanged to avoid environmental interference.

[0078] The experimental data were rounded to two decimal places.

[0079] Table 1 Data on the effect of the composition on improving memory

[0080]

[0081]

[0082] Note: “*” indicates comparison with model control group, p<0.05; “#” indicates comparison with composition 1, p<0.05; “&” indicates comparison with capsule 1, p<0.05.

[0083] According to the experimental results in Table 1, compositions 1-3 have significant effects on improving memory. By comparing composition 1 with compositions ①-④, it can be seen that in the composition containing Omega3 of the present invention, there is a certain synergistic effect between Omega3, locust bean extract, locust flower extract, and jujube extract; by comparing the results of composition 1 with composition ⑤, it can be seen that Omega3, locust bean extract, locust flower extract, and jujube extract in the present invention have better effects when used in a specific mass ratio; by comparing capsules 1-3 with composition 1, it can be seen that the encapsulation of polyglycerol ricinoleate, tocopherol acetate and soft capsules helps to promote the absorption of the composition containing Omega3. By comparing capsule 1 with capsule ①, it can be seen that polyglycerol ricinoleate can more significantly promote the absorption of the composition containing Omega3 than glyceryl monostearate.

[0084] The embodiments described above are part of the embodiments of the present invention, rather than all of the embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of the present invention claimed for protection.

Claims

1. A composition containing Omega 3, characterized in that: The composition comprises the following raw materials in parts by weight: Omega 3: 4-6 servings; Sophora bean extract: 10-13 parts; Sophora japonica extract: 5-7 parts; Ziziphus jujube extract: 20-25 parts.

2. The composition according to claim 1, characterized in that The composition comprises the following raw materials in parts by weight: Omega3: 5 parts; Sophora bean extract: 12 parts; Sophora japonica extract: 6 parts; Ziziphus jujube extract: 23 parts.

3. Use of the composition as claimed in claim 1 or 2 in the preparation of health care products.

4. The use according to claim 3, characterized in that: The health care product is a health care product containing Omega 3 and improving memory.

5. The application according to claim 4, characterized in that: The dosage form of the health care product is any one of capsules, tablets and granules.

6. A capsule containing Omega 3 for improving memory, characterized in that: The capsule comprises capsule contents and a capsule shell, and the capsule contents include the following components in mass percentage: 80-90wt% of the composition containing Omega 3 as claimed in claim 1 or 2; Polyglycerol polyricinoleate 4-5wt%; Tocopherol acetate 1-3wt%; Complete with drinking water.

7. A method for preparing the capsule according to claim 6, characterized in that: The preparation method comprises the following steps: S1: Evenly mix the Sophora bean extract, Sophora japonica flower extract, and Ziziphus jujuba extract with drinking water to obtain a mixed solution A; S2: Ultrasonic mixing of Omega 3, polyglycerol ricinoleate and tocopherol acetate to obtain a mixed solution B; S3: Mixing the mixed solution A and the mixed solution B, and performing ultrasonic vibration to make them uniform, to obtain the contents of the capsule; S4: Place the capsule contents in a capsule shell to prepare a capsule preparation, sterilize, seal, and store at room temperature away from light.

8. The method for preparing the capsule according to claim 7, characterized in that: The capsule preparation in step S4 is a soft capsule preparation.

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