Intestinal microecology regulator for short bowel syndrome

Abstract

The invention belongs to the crossing field of the agricultural fermentation industry field and food science, particularly relates to application of crops and derivatives thereof in a nutritional additive, and more particularly relates to an application of dietary fibers in the crops in body fermentation to regulation of intestinal microorganism distribution of short bowel syndrome patients. According to the present invention, the short bowel syndrome intestinal micro-ecological regulator comprises galactooligosaccharide, beta-glucan and pectin, such that the short-chain fatty acid can be generated in the intestinal environment of the short bowel syndrome patient, and the short-chain fatty acid can effectively regulate and improve the intestinal flora so as to promote the normal intestinal micro-ecological restoration of the short bowel syndrome patient. Therefore, the problems of intestinal environment disorder, digestive dysfunction, immune function decline and intestinal mucosa damage are solved.

Description

Technical Field

[0001] The present invention belongs to the intersection of the agricultural fermentation industry and food science, and particularly relates to the application of crops and their derivatives in nutritional additives. More specifically, it utilizes the in vivo fermentation of dietary fiber in crops to regulate the distribution of intestinal microorganisms in patients with short bowel syndrome, and uses it as an intestinal microecological regulator for short bowel syndrome. Background Art

[0002] Short bowel syndrome (SBS) refers to a syndrome in which the effective intestinal absorption area is significantly reduced after extensive small bowel resection or neglect due to various reasons, and the remaining functional intestine cannot maintain the patient's nutrition or children's growth needs, and symptoms such as diarrhea, acid-base imbalance, water and electrolyte disorders, and nutrient absorption and metabolism disorders appear. Short bowel syndrome is the most common cause of intestinal failure. After extensive intestinal resection, the gastrointestinal structure, motility, and hormone secretion change successively to adapt to the pathophysiological changes of the compensatory body, requiring the residual intestine to gradually compensate in structure and function. Clinically, most short bowel patients have problems such as poor nutrient absorption, intestinal mucosal barrier atrophy, and impaired immune function, and it is difficult to get rid of the support of enteral and parenteral nutrition. The economic burden and complications related to parenteral nutrition not only reduce the quality of life of patients, but may even cause death of patients. Therefore, improving the absorption function of the remaining small intestine and colon is crucial to improving the prognosis of patients with short bowel syndrome.

[0003] Dietary fiber is a kind of polysaccharide. At present, dietary fiber is divided into two categories in the art, namely soluble dietary fiber and insoluble dietary fiber, according to whether the dietary fiber is soluble in water. However, with the continuous deepening of research, the classification of dietary fiber based on different properties has gradually increased. For example, hemicellulose, pectin and resistant starch are classified as non-starch polysaccharides, and oligofructose, oligogalactose, oligoisomaltose and oligoxylose are classified as resistant oligosaccharides, as well as lignin. After different dietary fibers enter the human intestine, they are fermented under the action of intestinal flora, and fermentation products are produced during the fermentation and digestion process. These products further affect the distribution and quantity of intestinal flora.

[0004] For patients with short bowel syndrome, their intestinal function is disordered and absorption and metabolism are impaired, resulting in intestinal flora and digestion disorders, which in turn affect the intestinal mucosal barrier and intestinal immune function, causing further deterioration of intestinal failure. Summary of the invention

[0005] The present invention aims at the current short bowel syndrome intestinal flora homeostasis being broken, thereby causing a series of functional disorders, digestive disorders, decreased immune function, damaged intestinal mucosal barrier and other problems, and provides a short bowel syndrome intestinal microecological regulator, including oligogalactose, β-glucan and pectin.

[0006] Preferably, the added mass ratio of the galacto-oligosaccharide, β-glucan and pectin is 1:1:1.

[0007] Preferably, the total dosage of galacto-oligosaccharide, β-glucan and pectin is 9-14 g / day.

[0008] More preferably, the total dosage of the galacto-oligosaccharide, β-glucan and pectin is 3 g x 3 times / day.

[0009] More preferably, the present invention further discloses an enteral nutritional supplement containing the galacto-oligosaccharide, β-glucan and pectin.

[0010] Further preferably, the enteral nutritional supplement is a complete enteral nutritional supplement.

[0011] Among them, galacto-oligosaccharide is galacto-oligosaccharide derived from milk. In the prior art, the preparation of galacto-oligosaccharide includes enzymatic method and acid method, which uses lactose extracted from milk as raw material, and obtains galacto-oligosaccharide by galactase catalytic enzymolysis or acid catalytic lactose hydrolysis.

[0012] The β-glucan described herein is β-glucan extracted from oats. The β-(1→3, 1→4) glucan in oats is referred to as oat β-glucan, which is a non-starch polysaccharide existing in the cell walls of oat endosperm and aleurone layer. It is a high molecular polymer formed by connecting monomer β-D-pyranose glucose through β-(1→3) and β-(1→4) glycosidic bonds.

[0013] Meanwhile, the pectin is pectin extracted from citrus peel.

[0014] Pectin can be purchased directly or extracted as follows: clean fresh citrus peels and heat them in 90 degrees Celsius water for 10 minutes. After returning to room temperature, rinse the peels with water and cut them into 3 to 5 mm blocks. Rinse in hot water at 50±2℃ until the water becomes colorless and the peels have no odor. During the rinsing process, the peels need to be squeezed dry with nylon cloth before the next rinse. Then, put the treated peel blocks into a beaker and add 0.2mol / L dilute hydrochloric acid (the dilute hydrochloric acid completely covers the peels) to adjust the pH value of the solution to 2.0~2.5. Then, heat the mixture to 90℃ and maintain the temperature in a constant temperature water bath for 40 minutes, stirring continuously to ensure uniform heating. After heating, filter while hot using a Buchner funnel padded with nylon cloth (100 mesh) to collect the filtrate. Add 0.5%~1% activated carbon to the filtrate and decolorize it at 80℃ for 20 minutes. Filter while hot. Take the filtrate, cool it to room temperature, and adjust the pH value to 3-4 with ammonia water. Slowly add 95% alcohol solution under stirring until the alcohol mass fraction reaches 50%-60%, let it stand for 30 minutes, and filter it with 100-mesh nylon cloth to obtain pectin.

[0015] 5. Transfer the wet pectin to a 100 ml beaker and add 30 ml of anhydrous ethanol to wash. Then, filter it using a nylon cloth and squeeze it to remove the water. Put the dehydrated pectin into a watch glass and dry it at 60 to 70 degrees Celsius. Finally, grind and sieve the dried pectin to obtain dry pectin.

[0016] The invention discloses application of dietary fiber in preparing an intestinal microecological regulator for short bowel syndrome, and in particular, the dietary fiber is any one or more of galacto-oligosaccharide, beta-glucan and pectin.

[0017] The composite dietary fiber formula composed of three dietary fibers, galacto-oligosaccharide, β-glucan and pectin, disclosed in the present invention, can not only generate short-chain fatty acids in the intestinal environment of patients with short bowel syndrome, but also these short-chain fatty acids can effectively regulate and improve intestinal flora, and promote the restoration of normal microecology in the intestine of patients with short bowel syndrome. And then solve the problems of intestinal environment disorder, digestive dysfunction, immune function decline and intestinal mucosal damage caused by this. At the same time, the three dietary fibers, galacto-oligosaccharide, β-glucan and pectin, will not produce a large amount of gas in the intestinal simulated environment of patients with short bowel syndrome, and then will not cause abdominal distension and abdominal pain after use, and the comfort of use is high. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Schematic diagram of the experimental process of short bowel syndrome rat model.

[0019] Figure 2 Schematic diagram of body weight changes in model rats.

[0020] Figure 3 This is a schematic diagram of the diarrhea scoring results of model rats.

[0021] Figure 4 This is the staining result of intestinal pathological sections of model rats. DETAILED DESCRIPTION

[0022] In order to better understand the present invention, the present invention is further described below in conjunction with specific embodiments. Example 1

[0023] 1.1 Preparation of culture medium (per 1000 ml)

[0024] Measure 910ml of distilled water in a triangular flask 1 KCl 0.6 g 2 NaCl 0.6 g 3 <![CDATA[CaCl2·2H2O]]> 0.2 g 4 <![CDATA[MgSO4·7H2O]]> 0.5 g 5 <![CDATA[KH2PO4]]> 1.46 g 6 <![CDATA[Na2HPO4]]> 3.55 g Put the above drugs into the triangular flask in sequence and dissolve them in sequence. Prepare the microbial culture solution in the following order and proportion: (The following solutions are all added in 1L culture medium, and are added in sequence according to the preparation amount of the basic culture medium) 10 ml (A) trace element solution → 10 ml (E) hemin solution → 10 mL (F) fatty acid solution → 1 ml (D) resazurin solution → 50 ml (C) bicarbonate solution → heat in a microwave oven until boiling, pass CO2 to room temperature → add 1 g cysteine ​​hydrochloride before packaging → (adjust pH to about 6.8 with 0.2 M HCl and 0.2 M NaOH solution) → package 80 ml or 85 ml into fermentation bottles → add 1 ml (G) reducing agent Na2S and 1 ml (B) vitamin phosphate solution to each fermentation bottle → add 10 ml bacterial inoculum.

[0025] The preparation methods of A, B, C, D, E, F and G are as follows: A. Trace element solution: Add the following reagents in a 50mL beaker, dissolve with 10mL 0.02mol / L HCl, transfer to a 500mL volumetric flask and dilute with water. Dispense in 500mL brown bottles <![CDATA[MnCL2.4H2O]]> 12.5mg <![CDATA[FeSO4.7H2O]]> 10mg ZnCl2 12.5mg <![CDATA[CuCL2. 2H2O]]> 25mg <![CDATA[CoCL2.6H2O]]> 25mg <![CDATA[SeO2]]> 25mg <![CDATA[NiCl2. 6H2O]]> 125mg <![CDATA[Na2MoO4. 2H2O]]> 125mg <![CDATA[1111NaVO3]]> 15.65mg <![CDATA[H3BO3]]> 125mg B. Vitamin phosphate solution: First dissolve 27.35g KH2PO4 in 500 mL water to make a solution, then add the drugs in sequence. Before use, filter (filter with 2-micron filter paper) to sterilize, and store in a sterile test tube with a lid.

[0026] Biotin 10.2mg Folic acid 10.25mg Calcium D-pantothenate 82mg Nicotinamide 82mg Riboflavin 82mg Thiamin-HCl (Vitamin B1) 82mg Vitamin B6 Hydrochloride (pyridoxine-HCl) 82mg p-Aminobenzoic acid 10.2mg Cobalamin (VB12) 10.25mg C. Bicarbonate solution: weigh 82g of anhydrous Na2CO3 and add it to a 1L beaker. Add 1L of boiling water to dissolve. Keep aerating and use immediately. Aerate again for 20 minutes before use. (8.2g with 100ml of deionized water) D. Resazurin solution: (red in the presence of free oxygen, colorless in anaerobic conditions, used as an anaerobic indicator for this experiment). 0.1% (W / V): 100 mg of Resazurin dissolved in 100 ml of water, 100 ml beaker. (Add 1 ml of Resazurin per liter of culture medium) E. Hemin solution (500mL): Boil water in a clean water boiler; weigh 0.1g hemin and add it to a 10mL beaker, add 5ml 0.05mol / L NaOH to dissolve, transfer to a 500mL volumetric flask, continue to pass CO2, and make up to volume with boiling water, and continue to ventilate during the process. Dispense into 3 serum bottles.

[0027] F. Fatty acid solution (per 500ml): weigh 4g NaOH and add it to a 50mL beaker, dissolve it with a small amount of water, transfer it to a 500mL volumetric flask, add more than 300mL of water, shake well, add 3.43ml acetic acid, 1.50ml propionic acid, 0.92ml butyric acid and 0.28ml valeric acid to make up to 500ml. Dispense in 500mL brown bottles.

[0028] G. Preparation of reducing agent solution Weigh 20.5g Na2S·9H2O into a 50mL beaker, dissolve with a small amount of water, transfer to a 1L volumetric flask and make up to volume with boiling water, while continuously passing CO2 during the process. Filter and sterilize into a serum bottle and store at 4℃.

[0029] PBS can be purchased directly (10X PBS) or prepared as follows.

[0030] Phosphate buffer (pH 7.4, per 500ml water) NaCl 4.0g KCl 0.1g <![CDATA[Na2HPO4·2H2O]]> 0.72g <![CDATA[KH2PO4·2H2O]]> 0.12g 1.2 Collect colonic chyme from patients with short bowel syndrome in clinical practice.

[0031] 1.3 Mix the collected chyme and filter it with three layers of sterile gauze to obtain the inoculum. Use a sterile syringe to take 10 ml of the inoculum and inoculate it on the prepared fermentation medium, then place it on a shaker for anaerobic culture at a temperature of 39°C and a shaker speed of 50 r / min.

[0032] It was divided into four groups, three of which were experimental groups and one was a blank group. Six samples were set in each experimental group and three samples were set in the blank group. Galacto-oligosaccharide, β-glucan and pectin were added to the experimental groups respectively.

[0033] The pectin group was marked as P, the galacto-oligosaccharide group was marked as POS, the β-glucan group was marked as B, and the blank group was marked as C. Anaerobic culture was continued at a culture temperature of 39°C and a shaking speed of 50 r / min.

[0034] At the same time, the gas production was measured at 2h, 4h, 8h, 12h and 24h after fermentation according to the following method (since the metabolism time of pectin, oligosaccharides and β-glucan in the human body does not exceed 24 hours, we took 24 hours as the statistical endpoint) Cumulative gas production determination method: Preparation method:

[0035] (1) Check the integrity of the equipment: Before using the pressure converter to measure gas production, carefully check whether all parts of the equipment are complete and damaged. Check whether there are cracks on the casing of the pressure converter, whether the connecting pipes are intact and have no signs of aging or damage, and whether the interfaces are tight to avoid leakage during the measurement process that may affect the measurement results.

[0036] (2) Calibrate the instrument: Calibrate the instrument according to the instruction manual of the pressure converter. The calibration process generally requires the use of a standard gas with a known pressure, slowly passing the standard gas into the pressure converter, and adjusting the instrument parameters so that the pressure value displayed by the instrument matches the actual pressure value of the standard gas, ensuring the accuracy of the measurement data.

[0037] (3) Installation and connection of equipment: Install the gas pressure converter in a suitable location to ensure that it is stable and easy to operate. Connect its air inlet tightly to the air outlet of the gas production device (such as a fermentation tank, chemical reaction vessel, etc.) through a dedicated connecting pipe. When connecting, pay attention to the sealing of the interface to prevent gas leakage. If it is necessary to measure the gas at multiple gas production points, it is also necessary to connect the corresponding diversion device. Determination process:

[0038] (1) Turn on the gas generator: After ensuring that all connections are correct, start the gas generator and allow it to start generating gas. In the early stages of gas generation, closely observe the operating status of the gas generator and check for abnormal sounds, odors or other abnormal phenomena. If there is a problem, stop the gas generator immediately for inspection.

[0039] (2) Gas introduction and measurement: The gas generated by the gas production device enters the gas pressure converter through the connecting pipeline. The pressure sensitive element inside the gas pressure converter will sense the change in gas pressure and convert it into an electrical signal or other measurable signal. As the gas continues to enter, the pressure gradually rises, and the instrument will display the current gas pressure value in real time. During the measurement process, it is necessary to maintain a stable gas production state to avoid the gas production speed being too fast or too slow to affect the measurement accuracy.

[0040] (3) Recording data: According to the experimental requirements or actual monitoring needs, regularly record the pressure value displayed by the pressure converter. The recording time interval can be determined according to the gas production rate and the purpose of the experiment. For example, for reactions with faster gas production rates, it can be recorded every 1-2 minutes; for processes with slow gas production, it can be recorded every 5-10 minutes. The recorded data should include information such as measurement time and pressure value to ensure the integrity and accuracy of the data. End and follow-up processing

[0041] (1) Stop gas production: When the gas production measurement is completed or the termination condition set in the experiment is reached, first turn off the gas production device to stop gas production. Then slowly open the exhaust valve of the gas pressure converter (if any) to safely discharge the residual gas inside and restore the gas pressure converter to its initial state.

[0042] (2) Data collation and analysis: The recorded air pressure data is collated into a spreadsheet or other data analysis software, and a curve of air pressure changes over time is plotted. By analyzing the slope and trend of the curve, parameters such as the rate and total amount of gas produced can be calculated. If further information about the composition of the produced gas is required, other analytical methods such as gas chromatography may be required.

[0043] (3) Cleaning and maintenance of equipment: After the measurement is completed, clean the air pressure converter and connecting pipes. Use a clean soft cloth to wipe the equipment housing to remove dust and stains on the surface. For connecting pipes, they can be cleaned or replaced as needed to prevent residual gas from interfering with the next measurement. Store the equipment properly to avoid moisture, collision, etc. for next use.

[0044] The measurement results are shown in Table 1: Table 1

[0045] Example 2

[0046] The culture medium was prepared according to the preparation method in 1.1 of Example 1, the colonic chyme from clinical patients with short bowel syndrome was collected according to the method in 1.2 of Example 1, and the fermentation medium was obtained and cultured according to the method in 1.3 of Example 1.

[0047] The above culture medium was also divided into four groups, three of which were experimental groups and one was a blank group. Three samples were set in each group. Galactooligosaccharide, β-glucan and pectin were added to the experimental groups respectively.

[0048] The pectin group was marked as P, the galacto-oligosaccharide group was marked as POS, the β-glucan group was marked as B, and the blank group was marked as C. Anaerobic culture was continued at a culture temperature of 39°C and a shaking speed of 50 r / min.

[0049] Equal amounts of each fermentation broth were taken at 2 h, 4 h, 8 h, 12 h and 24 h after fermentation, and the fermentation broth was placed on ice to terminate the fermentation. The fermentation broth samples were then warmed to 4 °C at room temperature and centrifuged at 12.000 g for 5 min at this temperature. The supernatant was taken and crotonic acid was added as an internal standard (Cottyn and Boucque, 1968). The SCFA composition and content in the fermentation digestate were measured by the peak area method using a flame ionization detector and a capillary column (Subelco, column No. 34292-07 B, 30 m×0.32 mm×0.25 μm film thickness, USA). The detection conditions were: injector / detector temperature of 180 °C / 180 °C, column temperature of 140 °C, and gas flow rate of 30 ml / min.

[0050] The test results are shown in Table 2: Among them, A in "AB" represents the sample, and B represents the test time. For example, P1-2H represents the test result of the first culture medium of the pectin group 2 hours after fermentation, P2-2H represents the test result of the second culture medium of the pectin group 2 hours after fermentation, and P1-4H represents the test result of the first culture medium of the pectin group 4 hours after fermentation. Table 2 Acetic acid (μg / ml) Propionic acid (μg / ml) Isobutyric acid (μg / ml) Butyrate (μg / ml) Total short-chain fatty acids (μg / ml) P1-2H 4.60 0.73 0.00 0.45 5.79 P2-2H 4.71 0.59 0.00 0.59 5.89 P3-2H 3.84 0.74 0.00 0.39 4.97 POS1-2H 4.36 0.68 0.00 0.27 5.3 POS2-2H 3.57 0.88 0.00 1.00 5.45 POS3-2H 5.09 1.35 0.00 0.49 6.93 B1-2H 3.57 0.96 0.00 1.28 5.82 B2-2H 4.89 0.8 0.00 1.13 6.83 B3-2H 3.82 0.95 0.00 0.58 5.35 C1-2H 2.12 0.87 0.00 0.40 3.39 C2-2H 1.65 0.55 0.00 1.29 3.48 C3-2H 1.75 0.56 0.00 0.28 2.60 P1-4H 6.58 1.0 0.00 0.58 8.17 P2-4H 6.05 0.94 0.00 0.44 7.43 P3-4H 4.05 0.53 0.00 0.42 5.01 POS1-4H 12.96 1.97 0.00 1.70 16.63 POS2-4H 6.94 1.03 0.00 0.52 8.48 POS3-4H 6.65 0.79 0.00 0.77 8.21 B1-4H 12.15 3.56 0.00 1.37 17.08 B2-4H 4.01 1.08 0.00 1.35 6.44 B3-4H 4.36 0.59 0.00 0.41 5.36 C1-4H 11.43 2.93 0.00 1.47 15.82 C2-4H 2.64 0.65 0.00 0.41 3.70 C3-4H 9.09 1.39 0.00 0.37 10.85 P1-8H 13.62 4.7 0.00 0.44 18.77 P2-8H 31.37 4.07 0.00 0.54 35.98 P3-8H 16.65 4.53 0.00 2.2 23.39 POS1-8H 13.03 1.38 0.00 0.53 14.93 POS2-8H 12.47 0.78 0.00 0.45 13.69 POS3-8H 15.86 3.42 0.00 1.6 20.88 B1-8H 12.84 1.50 0.00 0.56 14.90 B2-8H 11.23 1.03 0.00 0.49 12.76 B3-8H 12.2 1.44 0.00 0.73 14.38 C1-8H 12.94 2.34 0.00 2.74 10.02 C2-8H 12.97 2.14 0.00 0.46 10.57 C3-8H 11.59 0.56 0.00 0.58 10.74 P1-12H 29.44 7.16 0.00 0.50 37.10 P2-12H 30.14 9.83 0.00 0.95 40.92 P3-12H 31.89 10.56 0.00 2.59 45.04 POS1-12H 17.23 2.66 0.00 1.32 21.2 POS2-12H 26.83 2.31 0.00 7.13 36.24 POS3-12H 26.53 1.65 0.00 0.46 28.63 B1-12H 16.55 6.20 0.00 0.80 23.55 B2-12H 18.30 2.85 0.00 0.78 21.92 B3-12H 19.74 2.75 0.00 0.73 23.22 C1-12H 7.82 2.57 0.00 1.02 11.40 C2-12H 6.78 1.36 0.00 0.68 8.82 C3-12H 4.70 35.17 0.00 6.95 6.83 P1-24H 109.21 42.15 0.00 4.07 155.43 P2-24H 85.73 30.79 0.00 3.04 119.57 P3-24H 109.74 42.0 0.00 2.62 154.37 POS1-24H 60.76 75.3 0.00 2.09 138.16 POS2-24H 72.0 65.70 0.00 7.47 145.18 POS3-24H 69.19 74.74 0.00 4.46 148.38 B1-24H 41.33 15.70 0.00 1.67 58.7 B2-24H 47.42 17.35 0.00 1.19 65.95 B3-24H 46.59 11.99 0.00 1.39 59.97 C1-24H 21.42 16.48 0.00 5.48 43.37 C2-24H 38.1 12.28 0.00 2.67 53.06 C3-24H 34.82 1.86 0.00 2.47 39.15 Example 3

[0051] Establishment of SD rat model of short bowel syndrome: 1. Anesthesia: Inject 1 ml of chloral hydrate intraperitoneally, wait for about 20 minutes, and weigh the body weight.

[0052] 2. Fixation: Fix the mouse's limbs on the operating table and prepare the skin.

[0053] 3. Prepare surgical instruments, four curved forceps, a needle holder, two tissue scissors, one toothless forceps, one toothed forceps, 6-0 and 3-0 needles and threads, and an electric scalpel. 4. Disinfect with iodine cotton balls for three times, and make an incision along the linea alba. Use an electric knife to coagulate the bleeding, and fix the skin on both sides with needle and thread to open the abdominal cavity. Spread the towel, cut four pieces of gauze soaked with saline, and spread them in the caudal, contralateral, cephalic, and proximal directions.

[0054] 5. First determine the location of the ileocecal region, and separate the small intestine 10 cm upward along the ileocecal region until it reaches about 10 cm from the Treitz ligament.

[0055] 6. Ligate the mesentery 10 cm above the ileocecal region in sections, ligating the intestinal tract and the two sides of the mesentery separately. Cut and sever the mesentery from the middle with an electric knife.

[0056] 7. Resection: Resect the ischemic and necrotic intestine and retain the active intestine at both ends.

[0057] 8. Anastomosis: Arrange the two ends of the intestinal tube neatly, butt them together and suture them. First, anastomose the two ends of the intestinal tube, fix them with a surgical knot, and then suture them continuously for about 8 stitches. Fold the intestinal tube over and suture 8 stitches again. After suturing, pull both sides of the intestinal tube to check whether the intestinal tube is airtight.

[0058] 9. Suture the abdominal cavity and the muscle layer continuously, with each stitch spaced about 0.5 cm apart. Then suture the skin intermittently, with each stitch spaced about 0.5 cm apart. 10. Disinfect again with iodine tincture.

[0059] A rat model with 70% intestinal resection was prepared according to the above method.

[0060] SD rats with 70% intestinal resection were divided into a control group (TP) and an intervention group (TPF). An equal number of SD rats were taken as a blank group (SHAM).

[0061] Experimental method reference Figure 1 .

[0062] The blank group was fed normally, and the control group and the intervention group were given 10% glucose in the first 3 days after surgery. Enteral nutrition was started 3 days later. The control group was only given enteral nutrition energy complete, and the intervention group was given enteral nutrition energy complete, and 15mg / ml of short bowel syndrome intestinal microbial adjustment (galacto-oligosaccharide, β-glucan, pectin mass ratio 1:1:1) was added to observe changes in intestinal barrier and intestinal immunity. According to the weight of rats, the daily energy requirement for each rat is about 80kcal, that is, 17.4g energy complete. From the first day after surgery, the food intake and weight changes of the mice were recorded daily, and the stool morphology, defecation frequency and special conditions in the stool were observed, specifically: Fecal morphology: According to the internationally accepted fecal scoring standard, rat fecal morphology is divided into different levels. Level 0 is normal feces, which is relatively dry and hard; Level 1 is soft feces but still has a certain shape; Level 2 is semi-formed feces, which is paste-like; Level 3 is completely unformed loose feces. Observe the feces of rats regularly every day and record the fecal morphology score of each rat. For example, "On the third day of the experiment, the fecal morphology score of rat A was level 2, showing semi-formed paste-like feces."

[0063] Defecation frequency: Quantify the defecation frequency by observing and recording the number of defecations of rats during a fixed time period (e.g., 8 a.m. to 10 a.m. every day). If the experimental period is long, it is necessary to keep the observation time consistent every day to reduce errors. When recording, indicate the number of each rat and the corresponding number of defecations, such as "On the 5th day of the experiment, rat B defecated 5 times between 8 and 10 a.m."

[0064] Special cases: Pay attention to whether there are abnormal substances such as blood and mucus in the feces. Once found, describe its characteristics in detail, such as the color of the blood (bright red, dark red, etc.), the amount (a small amount of blood, a large amount of blood, etc.), and the properties of the mucus (transparent, turbid, etc.). For example, "On the 7th day of the experiment, a small amount of bright red blood appeared in the feces of rat C, accompanied by a large amount of turbid mucus."

[0065] The results are as follows Figure 2 and Figure 3 As shown, it can be seen that after adding dietary fiber, the weight loss of mice gradually recovered and the diarrhea score was lower, while the group without dietary fiber addition did not regain weight and had a high diarrhea score.

[0066] At the same time, 14 days later, intestinal pathological sections were taken and stained to clarify the changes related to the intestinal barrier. Figure 4 , respectively showing the intestinal villi and barrier status of the jejunum, ileum, and colon. Figure 4 It can be seen that the intestinal villi morphology of the short bowel group (SBS group) was poor and the intestinal barrier was damaged, while the villi length of the dietary fiber group (Fiber+SBS group) recovered to be similar to that of the sham operation group (Sham group), and the villi width increased.

[0067] The above is a specific embodiment of the present invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications are considered to be within the protection scope of the present invention.

Claims

1. Intestinal microecological regulator for short bowel syndrome, characterized by: Includes galacto-oligosaccharides, beta-glucans and pectin.

2. The intestinal microecological regulator for short bowel syndrome according to claim 1, characterized in that: The added mass ratio of the galacto-oligosaccharide, beta-glucan and pectin is 1:1:

1.

3. The intestinal microecological regulator for short bowel syndrome according to claim 1, characterized in that: The total dosage of galacto-oligosaccharide, β-glucan and pectin is 9-14 g / day.

4. The intestinal microecological regulator for short bowel syndrome according to claim 1, characterized in that: The total dosage of the galacto-oligosaccharide, β-glucan and pectin is 3 g×3 times / day.

5. The intestinal microecological regulator for short bowel syndrome according to claim 1, characterized in that: Galacto-oligosaccharide is galacto-oligosaccharide extracted from milk.

6. The intestinal microecological regulator for short bowel syndrome according to claim 1, characterized in that: The β-glucan is β-glucan extracted from oats.

7. The intestinal microecological regulator for short bowel syndrome according to claim 1, characterized in that: The pectin is pectin extracted from citrus peel.

8. An enteral nutritional supplement comprising the short bowel syndrome intestinal microecological regulator according to any one of claims 1 to 7.

9. The enteral nutritional supplement according to claim 8, characterized in that: The enteral nutrition supplement is a complete enteral nutrition supplement.

10. The use of dietary fiber in the preparation of an intestinal microecological regulator for short bowel syndrome, characterized in that: The dietary fiber is any one or more of galacto-oligosaccharide, β-glucan and pectin.

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