Processing method of dunaliella salina

Abstract

The invention provides a dunaliella salina processing method. The dunaliella salina processing method comprises the following steps: collecting dunaliella salina, extracting dunaliella salina, and reusing a dunaliella salina extract. The method comprises the following steps: efficiently collecting dunaliella salina, carrying out classified extraction, and extracting different components of the dunaliella salina at one time by adopting an oil-water mixed extraction method to respectively obtain a dunaliella salina water-based extract, a dunaliella salina oil-based extract and a dunaliella salina extraction residue; the three different products enable the effective components of the dunaliella salina to be utilized to the maximum extent; and meanwhile, the product cost is greatly reduced. And the dunaliella salina eye wash is directly prepared from a dunaliella salina water extract and a dunaliella salina oil-soluble extract subjected to technical treatment under a sterile condition.

Description

Background technology

[0001] Dunaliella is a unicellular algae that has the ability to survive in extreme salinity conditions. Dunaliella is rich in a variety of nutrients, including nine-cis carotenoids, transparent carotene, β-carotene and other carotenoids, as well as polyunsaturated fatty acids, proteins, minerals, etc., and is therefore widely used in food, health products and cosmetics. However, there are some problems with the existing Dunaliella processing methods, such as the loss of nutrients, increased processing costs, and environmental pollution. Therefore, it is of great significance to develop an efficient, environmentally friendly and economical Dunaliella processing method. SUMMARY OF THE INVENTION

[0002] The present invention provides a method for processing salt algae, comprising the following steps:

[0003] Collection of salt algae: Collect salt algae grown in high-salinity environments such as salt lakes and salt pans; use high-efficiency concentration or membrane filtration to separate salt algae from brine through pipes and delivery pumps; concentrate the salt algae from 1 / 400 to 1 / 25; obtain salt algae paste; the innovation of this step is the use of high-speed concentration or membrane filtration methods, which completely distinguishes the long-standing flocculation and flotation collection methods;

[0004] Salina salt extraction: Extract the salt algae using the "oil-water" mixed extraction method. This extraction method is to use water, alcohol, oil-soluble extraction solvent acetone, etc. to extract different components of the salt algae at one time at one or several temperatures to obtain salt algae water-based, oily extracts, and salt algae extraction residues. The innovation lies in the use of an oil-water miscible extraction method to extract the oil and water-soluble extracts of the salt algae at one temperature;

[0005] Drying of salt algae: The different salt algae extracts obtained by extraction are dried separately to obtain salt algae powder and salt algae water-soluble concentrates. The salt algae oil extract is emulsified and microencapsulated to obtain the salt algae oil-soluble extract for use. The innovation lies in the upgrade of salt algae extraction technology, which makes it possible for different salt algae products to not have excessive heavy metals. Antioxidants and emulsifiers are added to the salt algae oil-soluble extracts, and then they are directly emulsified or spray-dried after emulsification to obtain oil-soluble microcapsules or water-soluble microcapsules of salt algae. This method protects the oil-soluble active ingredients of salt algae to the greatest extent, and maximizes the utilization of the active ingredients of salt algae; at the same time, it greatly reduces product costs.

[0006] Dunaliella finished product packaging: Dunaliella powder or Dunaliella water extract, and Dunaliella oil-soluble extract after technical treatment are packaged separately to obtain intermediate products or final products.

[0007] ​Production of Dunaliella salina eye wash: Mix the above-mentioned water-soluble extract of Dunaliella salina and the oil-soluble extract of Dunaliella salina treated by microcapsule technology through emulsification, sterilize them, and then dilute and fill them to obtain Dunaliella salina eye wash; this innovation is the application of new plant components that have never been used before in eye care products.

[0008] III. Advantages of the present invention:

[0009] Using Dunaliella salina in a high-salinity environment as raw material makes the nutritional components of the product more abundant and safer; through advanced collection and processing steps, impurities and harmful substances are removed, ensuring the quality and safety of the product; membrane filtration treatment is adopted to protect the nutritional components in Dunaliella salina to the greatest extent in the state of algal paste, and oil-water miscible extraction is carried out in this step, improving the utilization rate of raw material products; reducing the generation of harmful substances in the product; saving costs to the greatest extent.

[0010] Through the extraction step, water extract of Dunaliella salina, oil-soluble extract of Dunaliella salina, and Dunaliella salina powder are obtained, making the form of the product more diverse; reducing costs; and then compounding the terminal product Dunaliella salina eye wash, re-compounding the water-soluble concentrate of Dunaliella salina and the completely protected oil-soluble extract of Dunaliella salina and applying it to eye care products, which is a brand-new application. Some eye products related to Dunaliella salina extracts have entered clinical trials and have achieved unexpected therapeutic effects on cataracts and fundus macular lesions.

[0011] Adopting classified drying treatment makes the product have a longer shelf life and more stable quality; and more uses;

[0012] IV. Embodiment:

[0013] The embodiments of the present invention are only used to illustrate the present invention and are not used to limit the protection scope of the present invention. Within the protection scope of the present invention, those skilled in the art can modify and deform the specific embodiments of the present invention as needed.

[0014] Embodiment 1:

[0015] Collection of Dunaliella salina: In a 600-cubic-meter breeding pond, use a self-priming pump to pump the breeding liquid into the processing workshop through a pipeline. The pipeline is equipped with a primary 80-mesh sieve to filter impurities and collect Dunaliella salina growing in high-salinity environments such as salt lakes and salt fields. The processing workshop uses membrane filtration to process the collected algal liquid, and the passing rate of the membrane is 450 Dalton in molecular weight. Obtain algal paste, collect Dunaliella salina centrally, and protect the nutritional components in it to the greatest extent. The concentration ratio is 1 / 25, and about 24 liters of Dunaliella salina paste can be obtained from 600 cubic meters of breeding liquid.

[0016] Dunaliella salina extraction: (1) Feed 24 liters of Dunaliella salina paste into the extraction tank, and add an equal volume of 24 - 25 liters of water and five times the amount of approximately 120 liters of acetone oil-soluble extraction solvent to the extraction tank for extraction. The extraction time is 3 hours, the extraction temperature is 50 °C, and the stirring speed is 60 revolutions per minute. (2) After the extraction time is reached, open the valve at the lower part of the extraction tank and slowly release the Dunaliella salina water extract and Dunaliella salina flocs at the lower part of the extraction tank, and pump them into the centrifuge through the delivery pump at the lower part of the extraction tank. (3) After separation by the centrifuge, pump the Dunaliella salina water phase into the water phase evaporation tank to concentrate the water-soluble Dunaliella salina extract. The concentration temperature is 80 °C, the vacuum degree is -0.06 MPa, and the concentration time is 45 minutes. When the concentration time is up, break the vacuum and release the water-soluble Dunaliella salina extract from the lower part of the water phase evaporation tank and load it into the sealing device for standby; the preparation of the water-soluble Dunaliella salina extract is completed. (4) Collect and wash the Dunaliella salina solids separated by the centrifuge with purified water, and then send the remaining washed material into a vacuum drying oven for drying. The drying temperature is 80 °C, the vacuum degree is -0.06 MPa, and the drying time is 1 hour. After drying, Dunaliella salina powder is obtained. (5) Evaporate and recover acetone from the oil-soluble extract on the upper layer of the extraction tank at a vacuum degree of -0.06 MPa and a temperature of 80 °C; after continuously distilling and recovering for 1.5 - 2 hours, when the vacuum degree rises to -0.07 MPa and the temperature rises to 90 °C, the distillation of acetone is completed. Cool down the evaporation tank. When the temperature of the extraction tank drops to room temperature, remove the vacuum and release the oil-soluble Dunaliella salina extract from the lower part of the extraction tank. Through the above five steps, the oil and water extracts of Dunaliella salina are classified for evaporation recovery and the collection of solids, and the water extract of Dunaliella salina, the oil extract of Dunaliella salina, and Dunaliella salina powder are obtained respectively.

[0017] Example Two:

[0018] First step, open the valve of a 400 - cubic - meter Dunaliella salina cultivation pond, and pump the Dunaliella salina cultivation liquid into the liquid storage tank of the centrifuge through the delivery pump; start the centrifuge. After the rotation speed of the centrifuge reaches 4000 revolutions per minute, slowly release the Dunaliella salina cultivation liquid into the centrifuge at a flow rate of 10 milliliters per minute, and gradually increase the feed rate until the feed rate valve is fully opened to reach a treatment volume of 10 L / minute; after finishing processing 400 cubic meters of the cultivation liquid, send clean water into the storage tank to wash the storage tank and the centrifuge. After complete cleaning, stop the operation of the centrifuge to obtain the centrifuged algal paste.

[0019] Step 2: Dilute the above algal paste with purified water by a factor of two and send it into the extraction tank with a jacket using a transfer pump through a pipeline; simultaneously, use a vacuum pump to suck in anhydrous ethanol and petroleum ether in an amount five times that of the algal paste. After completing the above steps, close all the valves of the extraction tank and start heating. Stop heating when the temperature reaches 48 °C, keep it at a constant temperature of 50 °C, start stirring at a speed of 60 revolutions per minute, and extract for 3 hours. After the extraction time is reached, stop stirring, inject brine into the jacket for cooling, and let it stand still. Observe the layering situation through the level tube. After complete layering, first suck the Dunaliella salina flocs into Storage Tank 1, then suck the alcohol and water into Storage Tank 2, and finally close the feeding valve. Reheat the extraction tank and turn on the vacuum pump to maintain a vacuum degree of -0.06 MPa and a temperature of 70 °C to recover petroleum ether. Stop heating when the temperature rises to 80 °C and the vacuum degree rises to -0.08 MPa, and start cooling. When the temperature drops to room temperature, stop the vacuum and break the vacuum, then open the discharge valve to collect the material at the lower part. While distilling and recovering petroleum ether, distill and recover alcohol from the aqueous extract of Dunaliella salina. The time and temperature operation steps are the same as above to obtain the aqueous extract of Dunaliella salina. Separate the flocs of Dunaliella salina using a three-foot centrifuge or a disc separator to obtain Dunaliella salina mud and the aqueous extract of Dunaliella salina. Dilute the Dunaliella salina mud by a factor of two and send it into the drying tower. Either fluidized bed drying or centrifugal spray drying can be used. The inlet air temperature is between 180 and 230 °C, and the exhaust air temperature is between 60 and 80 °C to obtain Dunaliella salina powder. Combine the aqueous extract of Dunaliella salina with the above-mentioned aqueous extract of Dunaliella salina, and either package it or spray dry it after adding maltodextrin according to customer requirements to obtain the final concentrated aqueous extract of Dunaliella salina or the dry powder of the aqueous extract of Dunaliella salina.

Claims

1. The present invention provides a method for processing Dunaliella, comprising the following steps: Collection of salt algae: Collect salt algae grown in high-salinity environments such as salt lakes and salt pans; Use high-efficiency concentration or membrane filtration to separate salt algae and brine through pipelines and delivery pumps; concentrate the salt algae from 1 / 400 to 1 / 25; obtain salt algae paste; the innovation of this step is the use of high-speed concentration or membrane filtration method, which completely distinguishes the long-standing flocculation and flotation collection methods; Extraction of Dunaliella: Extract the Dunaliella using the "oil-water" mixed extraction method. This extraction method is to extract the different components of Dunaliella at one time using water, alcohol, oil-soluble extraction solvent acetone, etc. at one or several temperatures to obtain the Dunaliella aqueous extract, oily extract, and Dunaliella extraction residue respectively. The innovation lies in the use of an oil-water miscible extraction method to extract the oil- and water-soluble extracts of Dunaliella at one temperature; Drying of Dunaliella: Drying the different Dunaliella extracts obtained by extraction separately to obtain Dunaliella powder and Dunaliella water-soluble concentrate; emulsifying and microencapsulating the Dunaliella oil extract to obtain the Dunaliella oil-soluble extract to be used; The innovation lies in the upgrade of salt algae extraction technology, which ensures that different salt algae products will not have excessive heavy metals. After adding antioxidants and emulsifiers to the salt algae oil-soluble extract, it is directly emulsified or spray-dried after emulsification to obtain salt algae oil-soluble microcapsules or water-soluble microcapsule products. This method can best protect the oil-soluble active ingredients of salt algae and maximize the utilization of the active ingredients of salt algae; at the same time, it greatly reduces product costs. Packaging of finished products of Dunaliella: Dunaliella powder or Dunaliella water extract, and Dunaliella oil-soluble extract after technical treatment are packaged separately to obtain intermediate products or final products. Production of salt algae eye wash: The above-mentioned salt algae water-soluble extract and the salt algae oil-soluble extract treated with microencapsulation technology are mixed and sterilized before dilution and filling to obtain the salt algae eye wash; this innovation is the application of new plant ingredients that have never been seen before in eye care products.