Amplification method for domesticated population of predatory mites

Abstract

The invention relates to an amplification method for domesticated population of predatory mites, which is specifically characterized in that basal feed for predatory mites is added into feeding containers with different specifications, and the thickness of the basal feed accounts for 10-80% of the total height of a feeding box. Meanwhile, loose media including cottonseed hulls, pistachio shells, corrugated paper and the like are additionally added into the basal feed; oviposition media comprise wool, cotton threads, polyester fibers and the like, the predatory mite population can be amplified by more than 10 times within three weeks, and the number of adult mites can reach more than 30 per gram of feed. The feed structure can significantly affect population amplification in a high-density environment. Various loose media and spawning media are additionally added on the basis of the basic feed, the complexity of an inhabiting structure in a feed system is increased, more loose and porous activity spaces are provided, the predatory mites are evenly distributed in the feed, the feed and space resources are fully utilized, and finally the total population number is increased.

Description

Technical Field

[0001] The invention relates to the field of biological control of agricultural pests, and in particular to a method for increasing a domesticated population of predatory mites. Background Art

[0002] Pests are one of the important factors restricting the sustainable development of agriculture. At present, the misuse and abuse of chemical synthetic pesticides have caused a series of ecological and social problems. With the introduction of concepts and policies such as "sustainable agriculture" and "green plant protection", the goal of pest control has changed from complete elimination to control below the economic damage level, which has emphasized the importance of ecological significance, coordinated coexistence, multi-tactic prevention and control, and benefits to all parties. In this context, the development of green control technology for agricultural pests and diseases has been promoted. Among them, predatory mites, as a major category of natural enemies, generally have strong predation and search capabilities, large reproductive potential, wide food habits, and can coexist with prey. Pests can be controlled below the economic damage level without the use of pesticides. my country has a vast territory, rich predatory mite resources, a wide range of available species, and a wide range of hosts. Some predatory mites have achieved factory-based mass production. The low-cost and high-efficiency production characteristics make it possible to achieve flooded field release. The prevention effect of some predatory mites is even comparable to that of chemical pesticides.

[0003] Existing studies have shown that predatory mites can be artificially raised and their populations expanded by improving food structure and feeding methods. At the same time, improving the physical structure of feed can also facilitate expansion, and related research is still in the basic stage. In a fixed space, when the feed is too thick, predatory mites are easily contaminated by debris and dust due to their appearance characteristics, making it difficult for them to move through the thick feed. As a result, individuals gather on the surface of the feed, wasting a lot of food and space resources. In large-scale artificial breeding, high-density populations will be limited by food and available space, which will have a negative impact on the biological characteristics of the population. Increasing the complexity of the habitat structure in the feeding system, the looseness of the feed, and adjusting the ratio of feed thickness to feeding space are ways to solve the above problems. Therefore, it is necessary to continue to explore and develop methods in feed structure that are conducive to the expansion of predatory mites. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a method for expanding the domesticated population of predatory mites in response to the above problems and requirements.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0006] A method for expanding a domesticated population of predatory mites comprises the following steps:

[0007] Step 1: Select a feeding box as needed;

[0008] Step 2: Add basic feed for predatory mites into the breeding box. The thickness of the basic feed should account for 10% to 80% of the total height of the breeding box.

[0009] Step 3, select a loose medium and mix it into the basic feed, the addition amount is 1-20% of the basic feed mass;

[0010] Step 4: Add the spawning medium to the breeding box, the amount added is 1-20% of the volume of the basic feed;

[0011] Step 5: Add alternative prey and predatory mites to the breeding box, and place the breeding box in an artificial intelligence climate incubator for more than 3 weeks.

[0012] Furthermore, the breeding box is a rectangular breeding box or a cylindrical breeding box; the breeding box cover has holes and is covered with a breathable gauze with a mesh density of 300-400 meshes; and the breeding box is made of transparent material.

[0013] Furthermore, the loose medium is a natural loose medium or an artificial loose medium. The natural loose medium is any one or more of cottonseed shells, pistachio shells, sycamore shells, acorn caps, pine cones, hibiscus flowers, persimmon stalks, radish shells, peanut shells, Xanthium sibiricum, branches, citrus leaves, leaves damaged by leaf miners, walnut peels or shells; the artificial loose medium is any one or more of corrugated paper, cardboard, ring-shaped plastic or plastic sheets.

[0014] Furthermore, the egg-laying medium is made of any one or more of wool, hemp rope, cotton fiber, artificial chemical fiber, acrylic fiber, polyamide fiber or polyester fiber.

[0015] Furthermore, the predatory mites include any one or more of Amblyseius jiangyuanensis, Neoseius barkeri, Neoseius cucumeri and Euseius nissi.

[0016] After adopting the above technical solution, the present invention has the following advantages compared with the prior art:

[0017] The present invention obtains a method that is beneficial to the growth of predatory mite population from the aspect of feed structure, specifically the relationship between feed thickness and breeding space, and the method of adding loose medium and egg-laying medium. The existing publicly available conclusions and data mainly discuss the effects of food and temperature and humidity environment in the feed system on the growth of predatory mite population. There are no relevant reports on feed structure, and the specific amount of feed added is unclear. However, due to the limitations of morphological characteristics and behavioral influences of predatory mites, feed structure will significantly affect population expansion in a high-density environment. The present invention adds various loose media and egg-laying media to the basic feed, which increases the complexity of the habitat structure in the feed system, provides more loose and porous activity space, and allows predatory mites to be evenly distributed in the feed, making full use of feed and space resources, and ultimately increasing the total population.

[0018] The present invention is described in detail below with reference to the embodiments. DETAILED DESCRIPTION

[0019] The principles and features of the present invention are described below. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0020] Example 1

[0021] Step 1: Choose a breeding container

[0022] Cylindrical feeding box: The bottom diameter of the box is 100mm, and the height is 100mm. The cover of the feeding box has a breathable mesh with a hole diameter of 40mm and a mesh density of 300 mesh; the feeding box is transparent. Before adding feed, the feeding container is disinfected and sterilized by wiping with alcohol and irradiating with ultraviolet rays.

[0023] Step 2: Determine the amount of basic feed to be added

[0024] Each formula of the basic feed is disinfected and sterilized by ultraviolet irradiation, high temperature and high pressure. The basic feed of predatory mites is added to the breeding box, and the thickness of the basic feed accounts for 20% of the height of the box.

[0025] Step 3: Choose loose media

[0026] Choose natural loose medium pistachio shell. The loose medium is disinfected and sterilized by ultraviolet irradiation, high temperature and high pressure.

[0027] Step 4: Choose the Spawning Medium

[0028] The egg-laying medium is artificial chemical fiber (PP cotton), and the egg-laying medium is disinfected and sterilized by ultraviolet irradiation.

[0029] Step 5: Adding media

[0030] Mix the loose medium with the basic feed, and add 5% of the basic feed weight; mix the spawning medium into the feed, and add 5% of the basic feed volume. The loose medium and spawning medium are evenly distributed in the feed.

[0031] Step 6: Feeding of predatory mites

[0032] Add predatory mites and substitute prey to the breeding box, and place the breeding box in an artificial intelligence climate incubator with a temperature of 20-30℃, a relative humidity of 70-90%, and a light cycle of L:D=16:8. After 3 weeks of breeding, the population of predatory mites can increase by more than 10 times, and the number of adult mites can reach more than 30 heads / gram.

[0033] The specifications of the breeding container of the present invention can be adjusted at any time; the loose medium and the egg-laying medium can be applied to the practice of releasing predatory mites in the field in addition to being applied to artificial breeding conditions.

[0034] The method of the present invention can be applied to the large-scale artificial breeding of polyphagous predatory mites such as Amblyiseius eharai (Amitai et Swirski), Neoseiulus barkeri, Amblyseius cucumeris (Oudemans), and Euseius nicholsi (Ehara et Lee).

[0035] The above is an example of the best implementation of the present invention, and the parts not described in detail are common knowledge of ordinary technicians in the field. The protection scope of the present invention shall be based on the content of the claims, and any equivalent transformation based on the technical enlightenment of the present invention is also within the protection scope of the present invention.

Claims

1. A method for expanding the domesticated population of predatory mites, It is characterized in that The following steps are involved: Step 1: Select a feeding box as needed; Step 2, adding basic feed for predatory mites into the breeding box, the thickness of the basic feed accounting for 10%-80% of the total height of the breeding box; Step 3, select a loose medium and mix it into the basic feed, the addition amount is 1-20% of the basic feed mass; Step 4: Add the spawning medium to the breeding box, the amount added is 1-20% of the volume of the basic feed; Step 5: Add alternative prey and predatory mites to the breeding box, and place the breeding box in an artificial intelligence climate incubator for more than 3 weeks.

2. The method for increasing the domesticated population of predatory mites according to claim 1, It is characterized in that The breeding box is a rectangular breeding box or a cylindrical breeding box; the breeding box cover is perforated with a breathable gauze with a mesh density of 300-400 meshes; and the breeding box is made of transparent material.

3. The method for increasing the domesticated population of predatory mites according to claim 1, It is characterized in that The loose medium is a natural loose medium or an artificial loose medium. The natural loose medium is any one or more of cottonseed shells, pistachio shells, sycamore shells, acorn caps, pine cones, hibiscus flowers, persimmon stalks, radish shells, peanut shells, cockleburs, branches, citrus leaves, leaves damaged by leaf miners, walnut peels or shells; the artificial loose medium is any one or more of corrugated paper, cardboard, ring-shaped plastic or plastic sheets.

4. The method for increasing the domesticated population of predatory mites according to claim 1, It is characterized in that The egg-laying medium is any one or more of wool, hemp rope, cotton fiber, artificial chemical fiber, acrylic fiber, polyamide fiber or polyester fiber.

5. The method for increasing the domesticated population of predatory mites according to claim 1, It is characterized in that The predatory mites include any one or more of Amblyseius jiangyuanensis, Neoseius barkeri, Neoseius cucumeri and Euseius nissi.