Acaricidal composition and application thereof

Abstract

The invention belongs to the field of pesticides, and relates to an acaricidal composition and application thereof. The pesticide composition comprises an active component A and an active component B, the active component A is fluorothioether, and the active component B is chlorfenapyr. The composition has the advantages of synergism, resistance reduction and the like, and can be used for preventing and controlling agricultural and forestry pests and sanitary pests.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and in particular relates to application of an acaricidal composition in preventing and controlling agricultural and forestry harmful mites. Background Art

[0002] Fluomethiocarb is chemically named (4'-chloro-6-fluoro-4-methyl-[1,1'-biphenyl]-3-yl)(2,2,2-trifluoroethyl) sulfide, and its chemical structure is as follows:

[0003]

[0004] Chlorfenapyr is a new type of pyrrole compound, a highly effective insecticide and acaricide developed by the American Cyanamid Company in the late 1980s. Its mechanism of action is that after insects eat or come into contact with chlorfenapyr, it is oxidized and metabolized into a compound with insecticidal activity in the insect body, and then acts on the mitochondria of the insect cells, and acts through the multifunctional oxidase in the insect body, mainly inhibiting the conversion of adenosine diphosphate (ADP) to adenosine triphosphate (ATP), while adenosine triphosphate stores the energy necessary for cells to maintain their life functions, ultimately leading to the death of the pests.

[0005] Citrus spider mites are important pests in citrus production, including two major categories: Panonychus citri and Tetranychus citri. The adult, nymph and juvenile mites of Panonychus citri all harm citrus leaves, green branches and fruits, but the leaves are the most seriously damaged. The damaged leaves show many white spots and lose their luster. In severe cases, the whole leaves are gray and white, causing a large number of leaves to fall off; Tetranychus citri is a citrus leaf mite that harms citrus leaves, flower buds, fruits and tender shoots, with spring shoots being the most seriously damaged. The three insect stages of juvenile mites, nymph mites and adult mites gather on the main veins, branch veins and leaf edges on the back of the leaves to cause damage. Citrus spider mites have many generations, overlapping generations, long occurrence time, large occurrence, widespread occurrence, serious damage, and need long-term prevention and control. The irregular use of pesticides has led to the increasing resistance of citrus spider mites to pesticides. The screening of a new high-efficiency and low-toxic acaricide composition for the prevention and control of citrus spider mites by mixing two agents with different mechanisms of action is still an urgent problem to be solved in citrus production.

[0006] Through research and experiments, the present application surprisingly found that the mixture of fluazifop-butyl and chlorfenapyr has a good control effect on Tetranychidae pest mites, especially citrus spider mites, within a certain range, and has a synergistic effect, reducing the development of drug resistance of pest mites. In addition, in the prior art, the technical solution of the acaricidal composition containing fluazifop-butyl and chlorfenapyr has not been specifically disclosed. Summary of the invention

[0007] The present invention aims to provide a composition containing fluazifop-sulfuron and cypermethrin which has synergistic effect, reduces resistance and has low use cost.

[0008] To achieve the above object, the technical solution adopted by the present invention is: an acaricidal composition, the acaricidal composition comprising an active ingredient A and an active ingredient B, the active ingredient A is fluazifop-butyl, and the active ingredient B is chlorfenapyr;

[0009] Furthermore, the mass ratio of fluazifop to chlorfenapyr is 1:30 to 25:1;

[0010] Furthermore, the mass ratio of fluazifop to chlorfenapyr is 1:30, 1:24, 1:18, 1:12, 5:6, 4:3, 16:1, 25:1;

[0011] Furthermore, the mass ratio of fluazifop to chlorfenapyr is 1:24 to 25:1;

[0012] Furthermore, the mass ratio of fluazifop to chlorfenapyr is 1:24, 1:18, 1:12, 5:6, 4:3, 16:1, 25:1;

[0013] Furthermore, the mass ratio of fluazifop to chlorfenapyr is 1:18 to 16:1;

[0014] Furthermore, based on the total mass of the acaricidal composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the acaricidal composition is 0.01% to 90%;

[0015] Furthermore, based on the total mass of the acaricidal composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the acaricidal composition is 1% to 85%;

[0016] Furthermore, based on the total mass of the acaricidal composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the acaricidal composition is 5% to 80%;

[0017] Furthermore, the sum of the content of the active ingredient A and the active ingredient B in the acaricidal composition is 15%, 20%, 25%, and 44%;

[0018] Furthermore, the pesticide composition includes agriculturally acceptable auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers;

[0019] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, ethylene glycol polyoxyethylene polyoxypropylene ether, fatty alcohol ethoxylate, isomeric alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0020] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, block polyether phosphate salt, arylphenol polyoxyethylene ether sulfate salt, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acid, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0021] The emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, dodecylbenzene sulfonate amine, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, benzylphenol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol polyoxyethylene ether, alkylphenol ether phosphate and styrylphenol polyoxyethylene ether phosphate; and / or

[0022] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0023] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0024] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0025] The defoaming agent is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C 8 -C 10 One or more of fatty alcohols; and / or

[0026] The solvent is selected from one or more of benzene, toluene, xylene, trimethylbenzene, durene, methanol, ethanol, isopropanol, n-butanol, isooctyl alcohol, dimethyl sulfoxide, N,N-dimethylformamide, N,N-dimethylacetamide, N,N-dimethyldecylamide, N-methylpyrrolidone, cyclohexanone, propylene carbonate, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0027] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, butyl p-hydroxybenzoate, isothiazolinones, benzimidazoles, iodopropargyls, pyridinethiones, kasone and 1,2-benzisothiazolin-3-one; and / or

[0028] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0029] The synergist is selected from synergistic phosphorus, synergistic ether, synergistic amine; and / or

[0030] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0031] The invention optimizes the content of active ingredients and auxiliary agents in the acaricide composition to achieve a better balance between toxicity and residue, thereby enhancing the efficacy, reducing the dosage and lowering the cost.

[0032] Furthermore, the pesticide composition can be prepared into a formulation form permitted in agriculture, and the formulation form is selected from a solid formulation and / or a liquid formulation;

[0033] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0034] Further, the liquid preparation includes a soluble solution, a soluble gel, an oil, a film-spreading oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a fat, a suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion;

[0035] Furthermore, the solid preparation is selected from wettable powders and water dispersible granules; the liquid preparation is selected from soluble solutions, emulsifiable concentrates, aqueous emulsions, suspensions, suspoemulsions, microemulsions, and dispersible oil suspensions;

[0036] The present invention also discloses the use of the above-mentioned acaricide composition in preventing and controlling agricultural and forestry harmful mites;

[0037] Furthermore, the agricultural and forestry pest mites are Tetranychus, Mylidae and / or Eupodidae pest mites;

[0038] Furthermore, the Tetranychus mites include Tetranychus cinnabarinus, Panonychus citri, Tetranychus citri, Tetranychus urticae, Tetranychus urticae, and Tetranychus urticae.

[0039] Furthermore, the acaricidal composition is applied at an effective dose to the harmful mites to be controlled or to the medium where they grow.

[0040] Compared with the prior art, the beneficial effects of the technical solution of the present invention are as follows:

[0041] The acaricidal composition of the present invention has the following advantages:

[0042] 1) The acaricidal composition of the present invention exhibits a synergistic effect within a certain ratio range, thereby improving the control effect of the acaricidal composition on pests;

[0043] 2) The two active ingredients in the acaricide composition of the present invention have different action mechanisms, which effectively delay the development of insecticide resistance and extend the service life of the product;

[0044] 3) It is environmentally friendly, safe and efficient, and its promotion and application will have huge economic and social benefits. DETAILED DESCRIPTION

[0045] The present invention is further described below with reference to the examples, in which the percentages are all by weight, but the present invention is not limited thereto. The composition of the present invention can be provided in the form of a preparation.

[0046] According to the needs, it can be made into wettable powder, water-dispersible granules, soluble solution, emulsifiable concentrate, aqueous emulsion, suspension, suspoemulsion, microemulsion, dispersible oil suspension and the like.

[0047] The content of the active ingredient in the composition of the present invention depends on the dosage when used alone, as well as the mixing ratio and the degree of synergistic effect. The optimal range of the active ingredient content varies according to the formulation type of the composition.

[0048] Preparation Example:

[0049] Example 1: 44% fluazifop-chlorfenapyr wettable powder (20+24)

[0050] Formula: Fluomethiocarb 20%, chlorfenapyr 24%, naphthalene sulfonate 4%, polycarboxylate 1%, sodium lignin sulfonate 15%, sodium lauryl sulfate 3%, white carbon black 10%, kaolin added to 100%;

[0051] Preparation method: pre-crush and mix evenly, then grind by air flow mill to a fineness that satisfies the requirement that at least 98wt% passes through a 45μm test sieve to obtain a wettable powder.

[0052] Example 2: 40% fluazifop-chlorfenapyr water dispersible granules (20+20)

[0053] Formula: Fluomethiocarb 20%, chlorfenapyr 20%, lignin sulfonate 8%, α-olefin sulfonate 2%, naphthalene sulfonate formaldehyde condensate 8%, sodium lauryl sulfate 1.5%, white carbon black 1%, attapulgite 10.5%, bentonite added to 100%;

[0054] Preparation method: first mix evenly, then grind to less than 15μm by air flow mill, mix evenly with water, granulate by rotary granulator, dry at 60℃ to a moisture content of ≤3%, select 20-mesh and 60-mesh test sieves for sieving, discard the large particles on the top and the dust filtered at the bottom, and prepare water-dispersible granules.

[0055] Example 3: 32% fluazifop-chlorfenapyr suspension (17+15)

[0056] Formula: fluazifop 17%, chlorfenapyr 15%, naphthalene sulfonate formaldehyde condensate 0.5%, sodium lignin sulfonate 1%, EO-PO block copolymer 3.5%, polycarboxylate 2%, silicone defoamer 0.5%, xanthan gum 0.2%, magnesium aluminum silicate 1%, ethylene glycol 5%, sodium benzoate 0.5%, deionized water to 100%;

[0057] Preparation method: Add wetting dispersant and defoamer to the measured deionized water and stir evenly, then add active ingredients and stir evenly, use zirconium beads and sand mill to wet grind to D 90 (90% of the particle size) <5 μm, and a crushed slurry is obtained. A thickener, an antifreeze agent, and a preservative are added to the crushed slurry and mixed evenly, and deionized water is added to 100%, and a suspension is obtained by high-speed shearing.

[0058] Example 4: 26% fluazifop-chlorfenapyr suspoemulsion (10+16)

[0059] Formula: Fluomethiocarb 10%, chlorfenapyr 16%, lignin sulfonate 0.5%, polycarboxylate 1%, arylphenol polyoxyethylene ether phosphate 5%, EO-PO block copolymer 1.5%, alkylphenol polyoxyethylene ether 2%, ethylene glycol 2%, xanthan gum 0.2%, magnesium aluminum silicate 1%, silicone defoamer 0.5%, cyclohexanone 5%, solvent oil 5%, and deionized water to make up the balance;

[0060] Preparation method: According to the formula ratio, the active ingredient A is completely dissolved in the solvent, and the emulsifier is added to make the oil phase; the active ingredient B is mixed with a wetting dispersant, an antifreeze agent, a defoaming agent, deionized water, etc. by wet sand grinding to D 90 The suspension mother liquid is prepared by mixing the oil phase into the suspension mother liquid and stirring evenly, and then adding the thickener and stirring and shearing evenly to prepare the suspoemulsion preparation of the composition of the present invention.

[0061] Example 5: 15% fluazifop-chlorfenapyr EC (7+8)

[0062] Formula: Fluomethiocarb 7%, chlorfenapyr 8%, propylene carbonate 15%, sodium dodecylbenzene sulfonate 3%, styrylphenol polyoxyethylene ether 12%, N,N-dimethyldecylamide 12%, cyclohexanone 8%, and solvent oil to make up the balance;

[0063] Preparation method: firstly add the active ingredient into the solvent and completely dissolve it, then add the emulsifier and the like and stir evenly to form a uniform and transparent oily liquid, and then fill it to prepare the emulsifiable concentrate preparation of the composition of the present invention.

[0064] Indoor biological activity assay

[0065] Toxicity test 1

[0066] The combined toxicity of fluazifop-sulfuron and chlorfenapyr to Panonychus citri

[0067] The test is based on NY / T 1154.7-2006 "Guidelines for Indoor Organic Testing of Pesticides Part 7 Determination of Combined Effects of Mixtures".

[0068] Test insect source: Panonychus citri;

[0069] F 0 The sensitive stocks are provided by the Group’s R&D Center and are continuously raised indoors without exposure to chemicals;

[0070] F 5 Test population: Take female adult mites with the same development and use each test agent to treat F 0 The sublethal concentration was continuously treated for 4 generations, and the indoor F 5 The toxicity of female mites of the first generation was tested.

[0071] Test agents: 96% fluazifop technical and 98% chlorfenapyr technical, both provided by the Group’s R&D Center.

[0072] Preparation of test materials: Select female adult mites raised indoors and in the same physiological state. Cut the double-sided tape into 2 cm long pieces and stick them on one end of the slide. Then select healthy mites and stick their backs on the double-sided tape. Put 30 mites per slide into a container padded with a wet sponge, cover it with a lid, and place it at (25±1)℃. After 2 hours, examine under a microscope, remove dead and injured individuals, and make up 20 mites per slide.

[0073] Drug preparation: Dissolve the original drug in DMF and prepare high-concentration mother liquors. Then dilute the mother liquors with a 0.1% Tween-80 aqueous solution. Set 5 different concentrations for each treatment and keep them for later use.

[0074] Chemical treatment: Immerse the glass slide in the chemical solution and shake it gently for 5 seconds, then take it out and absorb the excess chemical solution with absorbent paper. Place it on a white porcelain plate lined with a wet sponge, cover it with a light-transmitting plastic film, and place it at (25±1)℃ for observation.

[0075] Experimental repetition: Each treatment was repeated 4 times, and a treatment without drug was set as a blank control.

[0076] Feeding and observation: The treated test insects were placed in a temperature of 25±0.5℃, a humidity of 60%-70%, and a photoperiod of L:D=16h:8h for feeding and observation. 48h after treatment, the number of dead mites was checked.

[0077] Data statistics and analysis: If the blank control mortality rate is less than 5%, no correction is required; if the blank control mortality rate is 5% to 10%, correction mortality rate is required; if the blank control mortality rate is more than 10%, the test needs to be repeated.

[0078] The calculation formula is as follows:

[0079]

[0080]

[0081] Theoretical toxicity index of mixture TTI = TI A ×P A +TI B ×P B

[0082]

[0083] Where: P A , P B are the proportions of active ingredients A and B in the composition, respectively.

[0084] Co-toxicity coefficient (CTC): CTC < 80 indicates antagonism, CTC > 120 indicates synergism, and between 80 and 120 indicates additive effect.

[0085] The DPS statistical analysis system was used to determine the LC of the toxicity regression line. 50 The activity of the test agent on the biological test material was evaluated by using the value and correlation coefficient r.

[0086] Resistance classification standards (Zhang Shuai et al., 2009 National Agricultural Pest Resistance Monitoring Results and Pesticide Use Recommendations, "China Plant Protection Guide", 2010, Vol. 30. No. 4):

[0087] A resistance ratio of <3 times is considered sensitive;

[0088] 3≤Resistance ratio<5 times is a state of decreased sensitivity;

[0089] 5≤Resistance ratio<10 times is low level resistance;

[0090] 10≤resistance ratio<40 times is medium level resistance;

[0091] 40≤resistance ratio<160 times is high level resistance;

[0092] A resistance ratio ≥160 times is an extremely high level of resistance.

[0093] Indoor test results:

[0094] As can be seen from Table 1, fluazifop-butyl and cypermethrin have a good combined effect on Panonychus citri. When the mass ratio of fluazifop-butyl to cypermethrin is 1:30-25:1, the co-toxicity coefficient is greater than 80, and no antagonism is shown. When the mass ratio of fluazifop-butyl to cypermethrin is 1:24-25:1, the co-toxicity coefficient is greater than 120, showing synergistic effect.

[0095] Table 1 Results of indoor toxicity test of fluazifop-butyl and chlorfenapyr alone or in combination against Panonychus citri (female adult mites)

[0096]

[0097]

[0098] As can be seen from Table 2, the sublethal concentrations of the tested agents in the treatment of citrus mites (sensitive population F 0 ), resulting in the corresponding resistance mechanism of citrus mites to the pesticides. The corresponding LC of fluazifop-sulfuron and cypermethrin alone or in combination against citrus mites 50 The value increases.

[0099] Table 2 Fluometra thiocarb and chlorfenapyr alone or in combination for the treatment of Panonychus citri (F 5The results of indoor toxicity test of

[0100]

[0101] The results in Table 3 show that the LC values ​​of the sensitive populations of each tested drug are 50 The values ​​were used as the baseline sensitivity of each test agent. The baseline sensitivity of Panonychus citri to fluazifop-butyl and chlorfenapyr were 26.492 mg·L -1 、167.260mg·L -1 , F 5 The resistance of Panonychus citri to single agents of fluazifop and cypermethrin showed an increasing trend, and was at a low level of resistance and a medium level of resistance, respectively; while the mass ratio of fluazifop to cypermethrin in the range of 1:24 to 16:1 was in a sensitive state or a state of reduced sensitivity.

[0102] Table 3 Analysis results of resistance of Panonychus citri to fluazifop and cypermethrin alone or in combination

[0103]

[0104] Toxicity test 2

[0105] The combined toxicity of fluazifop-sulfuron and cypermethrin mixed with tetracycline to Tetranychus cinnabarinus

[0106] The test is based on NY / T 1154.7-2006 "Guidelines for Indoor Organic Testing of Pesticides Part 7 Determination of Combined Effects of Mixtures".

[0107] Test insect source: Tetranychus cinnabarinus; provided by the Group's R&D Center, and continuously raised indoors without contact with pesticides;

[0108] Test agents: 96% fluazifop technical and 98% chlorfenapyr technical, both provided by the Group's R&D Center.

[0109] Preparation of test materials: Select nymphs raised indoors and in the same physiological state. Cut double-sided tape into 2 cm long pieces and stick them on one end of the slide. Then select healthy mites and stick their backs on the double-sided tape. Put 30 mites per slide in a container padded with a wet sponge, cover it, and place it at (25±1)℃. After 2 hours, examine under a microscope, remove dead and injured individuals, and make up 20 mites per slide.

[0110] Drug preparation: Dissolve the original drug in DMF and prepare high-concentration mother liquors. Then dilute the mother liquors with a 0.1% Tween-80 aqueous solution. Set 5 different concentrations for each treatment and keep them for later use.

[0111] Chemical treatment: Immerse the glass slide in the chemical solution and shake it gently for 5 seconds, then take it out and absorb the excess chemical solution with absorbent paper. Place it on a white porcelain plate lined with a wet sponge, cover it with a light-transmitting plastic film, and place it at (25±1)℃ for observation.

[0112] Experimental repetition: Each treatment was repeated 4 times, and a treatment without drug was set as a blank control.

[0113] Feeding and observation: The treated test insects were placed in a temperature of 25±0.5℃, a humidity of 60%-70%, and a photoperiod of L:D=16h:8h for feeding and observation. 48h after treatment, the number of dead mites was checked.

[0114] Data statistics and analysis: If the blank control mortality rate is less than 5%, no correction is required; if the blank control mortality rate is 5% to 10%, correction mortality rate is required; if the blank control mortality rate is more than 10%, the test needs to be repeated.

[0115] The calculation formula is as follows:

[0116]

[0117]

[0118] Theoretical toxicity index of mixture TTI = TI A ×P A +TI B ×P B

[0119]

[0120] Where: P A , P B are the proportions of active ingredients A and B in the composition, respectively.

[0121] Co-toxicity coefficient (CTC): CTC < 80 indicates antagonism, CTC > 120 indicates synergism, and between 80 and 120 indicates additive effect.

[0122] The DPS statistical analysis system was used to determine the LC of the toxicity regression line. 50 The activity of the test agent on the biological test material was evaluated by using the value and correlation coefficient r.

[0123] Indoor test results:

[0124] As can be seen from Table 4, fluazifop-butyl and cypermethrin have a good combined effect on Tetranychus cinnabarinus. When the mass ratio of fluazifop-butyl to cypermethrin is 1:24-25:1, the co-toxicity coefficient is greater than 120, showing a synergistic effect. When the mass ratio of fluazifop-butyl to cypermethrin is 1:18-25:1, the co-toxicity coefficient is greater than 130, and the synergistic effect is obvious.

[0125] Table 4 Indoor toxicity test results of fluazifop-sulfuron and chlorfenapyr against Tetranychus cinnabarinus (female adult mites)

[0126]

[0127] Field efficacy examples

[0128] Field test on the efficacy of pesticides against citrus red spider mites

[0129] Test crops: Citrus (Wogan);

[0130] Test subjects: Citrus spider mite;

[0131] Experimental design: 40% fluazifop-butyl·chlorfenapyr water dispersible granules (20+20), 32% fluazifop-butyl·chlorfenapyr suspension concentrate (17+15), 360 g / L chlorfenapyr suspension concentrate and 20% fluazifop-butyl suspension concentrate were used as control treatments, and clean water was used as a control.

[0132] Experimental replication and plots: Every 2 trees constituted a plot, with 5 treatments and 3 replications, for a total of 15 plots, arranged in random blocks, with one row of fruit trees between each plot.

[0133] Table 5 Test agents and dosage

[0134]

[0135] Test location: Citrus orchard in Suben Village, Liangqing District, Nanning City, Guangxi Zhuang Autonomous Region. The growth of citrus trees in the test area is basically the same, and citrus red spider mites have occurred more severely in recent years. The soil fertility of the test site is high, the cultivation and management conditions are good, and it is planted all year round.

[0136] Test time: The pesticide was applied on September 21, 2021. It was sunny on the day of application, with the highest temperature of 33°C, the lowest temperature of 25°C, and the east wind at level 1. The weather was cloudy during the test.

[0137] Application method: Use a motorized sprayer (WL-ABSC model) and manual spraying to evenly spray the entire tree.

[0138] Application times: Based on the actual field conditions and farmers' water usage habits, the water usage for each citrus plant is 1.8L, and the amount of application is determined so that the leaves are evenly covered with the drug and a little bit of the drug drips. Apply the drug once.

[0139] Survey method: Randomly select one point from the east, west, south, north and center of each citrus plant, mark a branch at each point, and record the number of all living citrus red spider mites on the leaves of the marked branches (starting from the top leaf, no less than 5 leaves). Mainly investigate adult mites and nymph mites (can be observed with a magnifying glass). Survey once before and 3d and 9d after the drug.

[0140] Calculation method of drug efficacy: The drug efficacy is calculated according to the following formula:

[0141]

[0142]

[0143] During the experiment, the citrus trees in each experimental plot grew well and no phytotoxicity was observed in any treatment.

[0144] The results of the field trials showed that:

[0145] As can be seen from Table 6, the mixture of fluazifop and cypermethrin has a good control effect on citrus red spider mites, showing good rapid effectiveness. 3 days after application, the control effects of 40% fluazifop·cypermethrin water dispersible granules (20+20) and 32% fluazifop·cypermethrin suspension (17+15) on citrus red spider mites were 90.62% and 90.30%, respectively. At the 0.05 level, through difference analysis, the mixed preparation of fluazifop and cypermethrin was significantly higher than the control single-dose 360 ​​g / L cypermethrin suspension and 20% fluazifop suspension.

[0146] Table 6 Control effect of each treatment on citrus red spider mites 3 days after application

[0147]

[0148] Note: The protective effect (%) in the table is the average value of each replicate, and the difference is at the 5% level. The same applies to the following tables.

[0149] As can be seen from Table 7, as time goes by, the control effect of fluazifop-sulfuron and cypermethrin on citrus red spider mites increases and the effective period is long. The control effects of 40% fluazifop-sulfuron·cypermethrin water dispersible granules (20+20) and 32% fluazifop-sulfuron·cypermethrin suspension (17+15) on citrus red spider mites are 95.91% and 95.20%, respectively. At the 0.05 level, through difference analysis, the mixed preparation of fluazifop-sulfuron and cypermethrin is significantly higher than the control single-dose 360 ​​g / L cypermethrin suspension and 20% fluazifop-sulfuron suspension.

[0150] Table 7 Control effect of each treatment on citrus red spider mites 9 days after application

[0151]

[0152] Note: The protective effect (%) in the table is the average value of each replicate, and the difference is at the 5% level. The same applies to the following tables.

[0153] Through indoor toxicity determination and tests on citrus in the field, the acaricidal composition of fluazifop-butyl and cypermethrin described in the present invention showed good control effect on phytophagous mites.

[0154] The pesticide composition or the preparation obtained by compounding the present invention has significant prevention effect, and is superior to a single agent in delaying the generation of drug resistance and prolonging drug retention. In addition, no compound agent was found to cause pesticide damage to crops in the test, indicating that the obtained pesticide composition or preparation can reduce production costs and use costs and is safe for crops when the insecticide synergistic synergy is improved.

[0155] Although the present invention has been described in detail above with general descriptions and specific implementation schemes, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A mite-killing composition, Features: The acaricidal composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is fluazifop-butyl and the active ingredient B is chlorfenapyr.

2. The acaricidal composition according to claim 1, Features: The mass ratio of fluazifop to chlorfenapyr is 1:30 to 25:

1.

3. The acaricidal composition according to claim 1, Features: The mass ratio of fluazifop to chlorfenapyr is 1:24 to 25:1; Preferably, the mass ratio of fluazifop to chlorfenapyr is 1:18 to 16:

1.

4. The acaricidal composition according to claim 1, Features: Based on the total mass of the acaricidal composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the acaricidal composition is 1% to 85%.

5. The acaricidal composition according to claim 1, Features: In addition to the active ingredients, the pesticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

6. The acaricidal composition according to claim 5, Features: The pesticide composition can be prepared into a formulation form permitted in agriculture, and the formulation form is selected from a solid formulation and / or a liquid formulation.

7. The acaricidal composition according to claim 6, Features: The solid preparation is selected from wettable powders and water-dispersible granules; the liquid preparation is selected from soluble solutions, emulsifiable concentrates, aqueous emulsions, suspensions, suspoemulsions, microemulsions, and dispersible oil suspensions.

8. Use of the acaricidal composition according to any one of claims 1 to 7 in controlling agricultural and forestry harmful mites.

9. The use according to claim 8, Features: The agricultural and forestry pest mites are Tetranychus, Acaridae and / or Eupodidae pest mites; Preferably, the pest mites of the Tetranychidae family include Tetranychus cinnabarinus, Panonychus citri, Tetranychus citri, Tetranychus urticae ... and Tetranychus urticae.

10. Use of the acaricidal composition according to claim 8, Features: The acaricidal composition is applied to the harmful mites to be controlled or the medium where they grow in an effective dosage.

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