A miticidal composition and use thereof

Abstract

The present application belongs to the field of pesticide insecticide, and relates to a kind of acaricidal composition and its application.The pesticide composition includes active ingredient A and active ingredient B, the active ingredient A is fluorine mite sulfide, and the active ingredient B is chlorfenapyr.The composition of the present application has the advantages of synergism and resistance reduction, and the pesticide composition can be used for preventing and treating agricultural and forestry pests, sanitary pests.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pesticides, and particularly relates to application of a kind of miticide composition in preventing and treating forest pests. BACKGROUND

[0002] Fluensulfone has a chemical name of (4'-chloro-6-fluoro-4-methyl-[1,1'-biphenyl]-3-yl)(2,2,2-trifluoroethyl) sulfide, and a chemical structural formula as follows:

[0003]

[0004] Chlorfenapyr is a new pyrrole compound, which is a high-efficiency insecticide and miticide developed by Cyanamid Company of the United States in the late 1980s. Its mechanism of action is that after insects ingest or contact chlorfenapyr, chlorfenapyr is oxidized and metabolized in the body of the insects to become a compound with insecticidal activity, and then acts on the mitochondria of cells in the body of the insects, and works through the multi-functional oxidase in the body of the insects, mainly inhibiting the conversion of adenosine diphosphate (ADP) to adenosine triphosphate (ATP), and adenosine triphosphate is necessary for the energy storage cells to maintain their life functions, and ultimately leads to the death of the pests.

[0005] Citrus red mite is an important pest of citrus production, including two major categories of citrus panmuga mite and citrus initial leaf mite. The adult, nymph and larva of the citrus panmuga mite all harm the leaves, green branches and fruits of citrus, but the harm to the leaves is the most serious. The harmed leaves present many white spots, lose luster, and the whole leaf is gray and white in severe cases, causing a large amount of leaf fall; the citrus initial leaf mite harms the leaves, flower buds, fruits and tender shoots of citrus, and the harm to the spring leaf is the most serious. The larva, nymph and adult of the mite cluster on the main veins, branch veins and leaf edges of the leaf back. The citrus red mite has multiple generations, generation overlap, long occurrence time, large occurrence amount, universal occurrence, serious harm, and needs to be controlled for a long time. Non-standard use of pesticides leads to stronger resistance of the citrus red mite to pesticides, and through the mixing of two kinds of pesticides with different mechanisms of action, a new miticide composition with high efficiency and low toxicity for controlling the citrus red mite is still an urgent problem to be solved in the current citrus production.

[0006] The present application surprisingly finds, through research experiments, that fluensulfone and chlorfenapyr mixed within a certain range have good control effect on the Tetranychidae mite, especially the citrus red mite, have a synergistic effect, and reduce the resistance of the mite. In addition, the technical solution of the miticide composition containing fluensulfone and chlorfenapyr is not specifically disclosed in the prior art. SUMMARY

[0007] The present application aims to provide a composition containing fluensulfone and chlorfenapyr, which has a synergistic effect, reduces resistance, and has low use cost.

[0008] To achieve the above object, the technical scheme adopted by the present application is as follows: a kind of acaricidal composition, the acaricidal composition includes active ingredient A and active ingredient B, the active ingredient A is fluorine mite sulfide, the active ingredient B is chlorfenapyr;

[0009] Further, the mass ratio of fluorine mite sulfide to chlorfenapyr is 1:30-25:1;

[0010] Further, the mass ratio of fluorine mite sulfide to chlorfenapyr is 1:30, 1:24, 1:18, 1:12, 5:6, 4:3, 16:1, 25:1;

[0011] Further, the mass ratio of fluorine mite sulfide to chlorfenapyr is 1:24-25:1;

[0012] Further, the mass ratio of fluorine mite sulfide to chlorfenapyr is 1:24, 1:18, 1:12, 5:6, 4:3, 16:1, 25:1;

[0013] Further, the mass ratio of fluorine mite sulfide to chlorfenapyr is 1:18-16:1;

[0014] Further, the sum of the content of active ingredient A and active ingredient B in the acaricidal composition is 0.01%-90% based on the total mass of the acaricidal composition being 100wt%;

[0015] Further, the sum of the content of active ingredient A and active ingredient B in the acaricidal composition is 1%-85% based on the total mass of the acaricidal composition being 100wt%;

[0016] Further, the sum of the content of active ingredient A and active ingredient B in the acaricidal composition is 5%-80% based on the total mass of the acaricidal composition being 100wt%;

[0017] Further, the sum of the content of active ingredient A and active ingredient B in the acaricidal composition is 15%, 20%, 25%, 44%;

[0018] Further, the pesticide composition includes one or more of wetting agent, dispersant, emulsifier, thickening agent, disintegrating agent, antifreeze, defoaming agent, solvent, preservative, stabilizer, synergist or carrier in addition to active ingredient, which is agriculturally acceptable auxiliary ingredient;

[0019] The wetting agent is selected from one or more of alkyl benzene sulfonate, alkyl naphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, dioctyl sodium sulfosuccinate, alpha olefin sulfonate, alkyl phenol ethoxylate, castor oil ethoxylate, glycol polyoxyethylene polyoxypropylene ether, fatty alcohol ethoxylate, isomeric alcohol ethoxylate, sodium fatty alcohol ethoxylate sulfate, silkworm excrement, soap nut, soapberry, SOPA, detergent, emulsifier 2000 series, and wetting penetrant F; and / or

[0020] The dispersing agent is selected from one or more of lignin sulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, styryl phenol ethoxylate phosphate ester, block polyether phosphate ester salt, aryl phenol polyoxyethylene ether sulfate ester, fatty alcohol ethoxylate, alkyl phenol polyoxyethylene ether, alkyl phenol polyoxyethylene ether formaldehyde condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylate, polyacrylate, phosphate, EO-PO block copolymer, and EO-PO graft copolymer; and / or

[0021] The emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, dodecylbenzene sulfonic acid amine, alkyl phenol formaldehyde resin polyoxyethylene ether, phenylethyl phenol polyoxyethylene polyoxypropylene ether, benzyl phenol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styryl phenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkyl phenol polyoxyethylene ether, alkyl phenol ether phosphate ester, and styryl phenol polyoxyethylene ether phosphate ester; and / or

[0022] The thickening agent is selected from one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and white carbon; and / or

[0023] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0024] The antifreezing agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0025] The antifoaming agent is selected from one or more of C 10 -C 20 saturated fatty acid compound, silicone oil, silicone compound, C8-C 10 fatty alcohol; and / or

[0026] The solvent is selected from one or more of benzene, toluene, xylene, trimethylbenzene, mesitylene, methanol, ethanol, isopropanol, n-butanol, isooctanol, dimethyl sulfoxide, N,N dimethylformamide, N,N dimethylacetamide, N,N dimethyldecanamide, N methylpyrrolidone, cyclohexanone, propylene carbonate, alkylene carbonate, diesel oil, solvent oil, vegetable oil, vegetable oil derivative, and water; and / or

[0027] The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, butyl p-hydroxybenzoate, isothiazolinone, benzimidazole, iodo propargyl, pyrithione, kathon, and 1,2-benzisothiazolin-3-one; and / or

[0028] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite, and starch; and / or

[0029] The synergist is selected from synergistic phosphorus, synergistic ether, and synergistic amine; and / or

[0030] The carrier is selected from one or more of ammonium salt, ground natural mineral, ground artificial mineral, silicate, resin, wax, solid fertilizer, water, organic solvent, mineral oil, vegetable oil, and vegetable oil derivative.

[0031] The present application optimizes the content of the effective ingredient and the adjuvant in the acaricidal composition, so that the toxicity and the residue reach a better balance, the drug efficacy is enhanced, the amount of drug used is reduced, and the cost is lowered.

[0032] Further, the pesticide composition can be prepared into an agriculturally allowable formulation form, which is selected from a solid formulation and / or a liquid formulation.

[0033] Further, the solid formulation includes a powder, a granule, a pellet, a tablet, a strip, a wettable powder, an oil dispersible powder, a milk powder, a water dispersible granule, a milk granule, a water dispersible tablet, a soluble powder, a soluble tablet, or a soluble granule.

[0034] Further, the liquid formulation includes a soluble liquid, a soluble gel, an oil, an oil spread, an emulsion, a latex, a dispersible liquid, a paste, a water emulsion, an oil emulsion, a microemulsion, a lipid, a suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspension emulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion, or a microcapsule suspension-suspension emulsion.

[0035] Further, the solid preparation is selected from wettable powder, water dispersible granule; the liquid preparation is selected from soluble liquid, emulsifiable concentrate, water emulsion, suspension, suspoemulsion, microemulsion, dispersible oil suspension and the like.

[0036] The application further discloses application of the acaricidal composition in prevention and treatment of agricultural and forestry acarids.

[0037] Further, the agricultural and forestry acarids are Tetranychidae, Eriophyidae and / or Cheyletidae acarids.

[0038] Further, the Tetranychidae acarids include Tetranychus cinnabarinus, Panonychus citri, Eotetranychus sp., Tetranychus urticae and Hemiberlesia latania.

[0039] Further, the acaricidal composition is applied on the acarids to be controlled or the medium where the acarids grow in an effective dose.

[0040] Compared with the prior art, the application has the following beneficial effects:

[0041] The acaricidal composition has the following advantages:

[0042] 1) The acaricidal composition has a synergistic effect within a certain ratio range, thereby improving the prevention and treatment effect of the acaricidal composition on pests.

[0043] 2) The two active ingredients in the acaricidal composition have different action mechanisms, thereby effectively delaying the generation of pest resistance and prolonging the service life of the product.

[0044] 3) The acaricidal composition is environment-friendly, safe and efficient, and has great economic and social benefits. DETAILED DESCRIPTION

[0045] The application is further described below in combination with examples, and the percentages in the examples are all weight percentages, but the application is not limited thereto. The composition of the application can be provided in the form of a preparation.

[0046] The wettable powder, water dispersible granule, soluble liquid, emulsifiable concentrate, water emulsion, suspension, suspoemulsion, microemulsion, dispersible oil suspension and the like can be prepared according to the requirements.

[0047] The content of the active ingredient in the composition of the application depends on the application amount when used alone, and also depends on the mixing ratio and the degree of synergistic effect. The optimal range of the content of the active ingredient is different according to the type of the composition.

[0048] Preparation example of the preparation:

[0049] Example 1: 44% fluoramiduthiofene and chlorfenapyr wettable powder (20+24)

[0050] Formulation: Sufosuidide 20%, Chlorfenapyr 24%, Naphthalene sulfonate 4%, Polycarboxylate 1%, Sodium lignosulfonate 15%, Sodium dodecyl sulfate 3%, White carbon black 10%, Kaolin added to 100%;

[0051] Preparation method: First, mix uniformly, then pass through the airflow pulverizer to be pulverized to a fineness that meets the requirement that at least 98wt% passes through the 45μm test sieve, to produce wettable powder.

[0052] Example 2: 40% Sufosuidide·Chlorfenapyr Water Dispersible Granules (20+20)

[0053] Formulation: Sufosuidide 20%, Chlorfenapyr 20%, Lignosulfonate 8%, Alpha-olefin sulfonate 2%, Naphthalene sulfonate formaldehyde condensate 8%, Sodium dodecyl sulfate 1.5%, White carbon black 1%, Attapulgite 10.5%, Bentonite added to 100%;

[0054] Preparation method: First, mix uniformly, then pass through the airflow pulverizer to be pulverized to 15μm or less, mix uniformly with water, pass through the rotary granulator to be granulated, dry at 60°C until the water content is ≤3%, select 20 mesh and 60 mesh test sieves for screening, discard the upper large particles and the bottom filtered dust, to produce water dispersible granules.

[0055] Example 3: 32% Sufosuidide·Chlorfenapyr Suspension Concentrate (17+15)

[0056] Formulation: Sufosuidide 17%, Chlorfenapyr 15%, Naphthalene sulfonate formaldehyde condensate 0.5%, Sodium lignosulfonate 1%, EO-PO block copolymer 3.5%, Polycarboxylate 2%, Silicone antifoaming agent 0.5%, Xanthan gum 0.2%, Magnesium aluminum silicate 1%, Ethylene glycol 5%, Sodium benzoate 0.5%, Deionized water added to 100%;

[0057] Preparation method: Add wetting dispersant and antifoaming agent to the measured deionized water and stir uniformly, then add the active ingredients and stir uniformly, use zirconium beads, and use a sand mill to wet grind to D 90 (90% of the particle size of the particles) <5μm, obtain the pulverized slurry. Add thickening agent, antifreeze agent, and preservative to the pulverized slurry and mix uniformly, add deionized water to 100%, and shear uniformly at high speed to produce the suspension concentrate.

[0058] Example 4: 26% Sufosuidide·Chlorfenapyr Suspension Emulsion Concentrate (10+16)

[0059] Formula: Sulprofos 10%, Chlorfenapyr 16%, Lignosulfonate 0.5%, Polycarboxylate 1%, Arylphenol polyoxyethylene ether phosphate ester salt 5%, EO-PO block copolymer 1.5%, Alkylphenol polyoxyethylene ether 2%, Ethylene glycol 2%, Xanthan gum 0.2%, Magnesium aluminum silicate 1%, Silicone defoamer 0.5%, Cyclohexanone 5%, Solvent oil 5%, Deionized water to make up the balance;

[0060] Preparation method: according to the formula proportion, the active ingredient A is completely dissolved with the solvent, and the emulsifier is used to prepare the oil phase; the active ingredient B is wet ground with wetting dispersant, antifreezing agent, defoamer, deionized water and the like to D 90 less than 5 μm to prepare the suspension mother liquor; the oil phase is added to the suspension mother liquor, stirred uniformly, then the thickening agent is added and stirred and sheared uniformly, so that the emulsifiable concentrate preparation of the present application is prepared.

[0061] Example 5: 15% sulprofos·chlorfenapyr emulsifiable concentrate (7+8)

[0062] Formula: Sulprofos 7%, Chlorfenapyr 8%, Propylene carbonate 15%, Sodium dodecylbenzenesulfonate 3%, Styryl phenol polyoxyethylene ether 12%, N, N-dimethyl decanamide 12%, Cyclohexanone 8%, Solvent oil to make up the balance;

[0063] Preparation method: the active ingredient is first added to the solvent to completely dissolve, then the emulsifier is added and stirred uniformly to form a uniform transparent oily liquid, which is filled, so that the emulsion oil preparation of the present application is prepared.

[0064] Indoor biological activity determination

[0065] Toxicity test one

[0066] Joint toxicity of sulprofos and chlorfenapyr to panonychus citri

[0067] The test is based on NY / T 1154.7-2006 "Pesticide indoor biological test guidelines part 7 determination of joint action of mixture".

[0068] Test insect source: panonychus citri;

[0069] The F0 sensitive population is provided by the group research and development center and is continuously bred in the room without contacting the pesticide;

[0070] F5 test population: the female adult mites with consistent development are treated with the sublethal concentration of each test pesticide for 4 generations, and the female adult mites of the indoor F5 generation are used for toxicity determination.

[0071] Test pesticide: 96% sulprofos technical material and 98% chlorfenapyr technical material, both of which are provided by the group research and development center.

[0072] Test preparation: Select the indoor breeding, physiological state of female adult mites. The double-sided tape is cut into 2 cm long, and is pasted on one end of the glass slide. Then, healthy mites are selected, and the back of each mite is pasted on the double-sided tape, 30 mites per piece. The mites are placed in a container with wet sponges, covered with a lid, and placed in a (25±1)℃ environment. After 2 hours, the dead and injured individuals are removed, and 20 mites are added to each piece.

[0073] Preparation of the agent: The original drug is dissolved in DMF to prepare a high-concentration mother liquor. The mother liquor is then diluted with 0.1% Tween-80 aqueous solution. Five different concentrations are set for each treatment.

[0074] Agent treatment: The glass slide is immersed in the drug solution and gently shaken for 5 seconds. Excess drug solution is absorbed with a water-absorbing paper, and the glass slide is placed in a white porcelain dish with wet sponges. The dish is covered with a plastic film with good light transmission and placed in a (25±1)℃ environment for observation.

[0075] Test repetition: Each treatment is repeated 4 times, and a blank control without the agent is set.

[0076] Feeding and observation: The treated insects are placed in an environment with a temperature of 25±0.5℃, humidity of 60%-70%, and light cycle of L:D=16h:8h for feeding and observation. After 48 hours of treatment, the number of dead mites is checked.

[0077] Data statistics and analysis: If the mortality rate of the blank control is less than 5%, no correction is needed. If the mortality rate of the blank control is between 5% and 10%, the corrected mortality rate is needed. If the mortality rate of the blank control is more than 10%, the test needs to be repeated.

[0078] The calculation formula is as follows:

[0079]

[0080]

[0081] Theoretical toxicity index of the mixture TTI = TI A × P A + TI B × P B

[0082]

[0083] In the formula, P A , P B are the proportions of active ingredients A and B in the composition, respectively.

[0084] Co-toxicity coefficient (CTC): CTC <80 is antagonistic, CTC >120 is synergistic, and between 80 and 120 is additive.

[0085] LC value and correlation coefficient r of the toxicity regression line were calculated by DPS statistical analysis system, and the activity of the test agents on the biological test material was evaluated. 50

[0086] Resistance classification criteria (Zhang Shuai et al., 2009 National Agricultural Pest Resistance Monitoring Results and Drug Recommendations, China Plant Protection Guide, 2010, Vol. 30. No. 4):

[0087] Resistance ratio < 3 times is sensitive state;

[0088] 3 ≤ resistance ratio < 5 times is sensitive decline state;

[0089] 5 ≤ resistance ratio < 10 times is low-level resistance;

[0090] 10 ≤ resistance ratio < 40 times is medium-level resistance;

[0091] 40 ≤ resistance ratio < 160 times is high-level resistance;

[0092] Resistance ratio ≥ 160 times is extremely high-level resistance.

[0093] Laboratory test results:

[0094] As can be seen from Table 1, fluensulfone and bifenenthrin have good joint action on Panonychus citri, and the co-toxicity coefficients are greater than 80 when the mass ratio of fluensulfone to bifenenthrin is 1:30-25:1, without showing antagonism. When the mass ratio of fluensulfone to bifenenthrin is 1:24-25:1, the co-toxicity coefficients are greater than 120, showing synergistic effect.

[0095] Table 1 Indoor toxicity determination results of fluensulfone and bifenenthrin single agent or mixture on Panonychus citri (female adult)

[0096]

[0097]

[0098] As can be seen from Table 2, the sublethal concentration of each test agent successively treated Panonychus citri (sensitive population F0) leads to the corresponding resistance mechanism of Panonychus citri to the agents, and the corresponding LC 50 values of Panonychus citri to fluensulfone and bifenenthrin single agent or mixture increase.

[0099] Table 2 Indoor toxicity determination results of fluensulfone and bifenenthrin single agent or mixture on Panonychus citri (F5 generation)

[0100]

[0101] Table 3 shows that the LC 50 ​The values were the sensitive baseline of each test agent, and the sensitive baseline of T. citri to flufenerim and buprofezin were 26.492 mg·L -1 , 167.260 mg·L -1 The resistance of F5 T. citri to flufenerim and buprofezin single agent showed a rising trend, and was at low-level resistance and medium-level resistance, respectively. However, flufenerim and buprofezin in the mass ratio of 1:24-16:1 were in a sensitive state or a sensitive decline state.

[0102] Table 3 Resistance analysis results of T. citri to flufenerim and buprofezin single agent or mixture

[0103]

[0104] Toxicity test two

[0105] Joint toxicity of flufenerim and buprofezin mixture to T. cinnabarinus

[0106] The test was based on NY / T 1154.7-2006 "Guidelines for Laboratory Tests of Pesticides Part 7 Determination of Joint Action of Mixtures".

[0107] Test insect source: T. cinnabarinus, provided by the Group R&D Center, and continuously bred in the room without contacting pesticides;

[0108] Test agents: 96% flufenerim technical material and 98% buprofezin technical material, both provided by the Group R&D Center.

[0109] Test material preparation: Select the nymphs bred in the room and with consistent physiological state. Cut the double-sided tape into 2 cm long, paste it on one end of the glass slide, then select healthy mites and paste their backs on the double-sided tape, 30 per piece, put them in a container with wet sponge, cover the lid, and place it in a (25±1) ℃ condition. After 2 h, examine under a microscope, remove dead and injured individuals, and supplement 20 mites per piece.

[0110] Pesticide preparation: Dissolve the technical material with DMF to prepare high-concentration stock solution, and then dilute the stock solution with 0.1% Tween-80 aqueous solution. Set 5 different concentrations for each treatment, and prepare for use.

[0111] Pesticide treatment: After immersing the glass slide in the pesticide solution and gently shaking for 5 s, remove the excess pesticide solution with a water-absorbing paper, place it in a white porcelain dish with wet sponge, cover it with a plastic film with good light transmission, and place it in a (25±1) ℃ incubator for observation.

[0112] Test repetition: Each treatment was repeated 4 times, and a blank control without pesticide was set.

[0113] Raising and observation: The treated insects were raised and observed under the conditions of temperature 25±0.5℃, humidity 60%-70%, and light cycle L:D=16h:8h. The number of dead insects was checked 48h after treatment.

[0114] Data statistics and analysis: The blank control mortality less than 5% need not be corrected; the blank control mortality 5%-10% needs to be corrected; the blank control mortality more than 10% needs to be retested.

[0115] The calculation formula is as follows:

[0116]

[0117]

[0118] Theoretical toxicity index TTI of mixture = TI A ×P A + TI B ×P B

[0119]

[0120] In the formula, P A , P B are the proportions of active ingredients A and B in the composition, respectively.

[0121] Co-toxicity coefficient (CTC): CTC<80 is antagonism, CTC>120 is synergism, and between 80 and 120 is additive effect.

[0122] The DPS statistical analysis system was used for analysis to obtain the LC 50 values and correlation coefficient r of the toxicity regression line, and to evaluate the activity of the tested agents on the biological test material.

[0123] Indoor test results:

[0124] As shown in Table 4, fluensulfone and bifenenthrin have good joint action on T. cinnabarinus. When the mass ratio of fluensulfone to bifenenthrin is 1:24-25:1, the co-toxicity coefficient is greater than 120, showing synergistic effect. When the mass ratio of fluensulfone to bifenenthrin is 1:18-25:1, the co-toxicity coefficient is greater than 130, showing obvious synergistic effect.

[0125] Table 4 Indoor toxicity determination results of fluensulfone and bifenenthrin mixture on T. cinnabarinus (female adult)

[0126]

[0127] Field efficacy examples

[0128] Field efficacy test of preventing and treating citrus red mite

[0129] Test crop: Citrus (Valencia orange) ;

[0130] Test object: Citrus red mite;

[0131] Test design: 40% fluoromethane sulfide · buprofezin water dispersible granules (20+20), 32% fluoromethane sulfide · buprofezin suspension concentrate (17+15), set 360 g / L buprofezin suspension concentrate, 20% fluoromethane sulfide suspension concentrate as control treatment, set water control.

[0132] Test repetition and plot: 2 trees per plot, 5 treatments, 3 repetitions, a total of 15 plots, using randomized block arrangement, with one row of fruit trees between plots.

[0133] Table 5 Test agents and dosage

[0134]

[0135] Test site: Su'pencun citrus garden in Liangqing District, Nanning City, Guangxi Zhuang Autonomous Region, the test area citrus trees grow basically the same, citrus red mite has been more serious in recent years. The test site has high soil fertility, good cultivation and management conditions, and is planted all year round.

[0136] Test time: September 21, 2021, the weather was sunny on the day of application, the maximum temperature was 33℃, the minimum temperature was 25℃, the east wind was 1 level, and the weather during the test was cloudy.

[0137] Application method: using a motorized sprayer (WL-ABSC type), manual spraying, uniform spraying of the whole tree.

[0138] Application frequency: according to the actual field and the habit of using water by farmers, the water consumption per citrus tree was determined to be 1.8 L, and the spraying amount was determined by the uniform spraying of leaves and the dripping of drug drops. Spray 1 time.

[0139] Investigation method: randomly select a point on the east, west, south, north and center of each citrus tree, mark a branch at each point, and investigate and record the total number of citrus red mites on the marked leaves (top leaves are counted, not less than 5 leaves). Mainly investigate adult mites and nymphs (can be observed with a magnifying glass). Investigate 1 time before and 3d, 9d after drug application.

[0140] Method for calculating efficacy: efficacy is calculated according to the following formula:

[0141]

[0142]

[0143] During the test, the growth of citrus trees in each test plot was good, and no phytotoxicity was observed in each treatment.

[0144] The results of field tests show that:

[0145] As can be seen from Table 6, the fluensulfone and buprofezin mixed preparation has good control effect on red spider mites of citrus trees, and shows good quick-acting property. On the 3rd day after treatment, the control effects of 40% fluensulfone·buprofezin water dispersible granules (20+20) and 32% fluensulfone·buprofezin suspension (17+15) on red spider mites of citrus are 90.62% and 90.30% respectively. At the 0.05 level, through difference analysis, the fluensulfone and buprofezin mixed preparation is significantly higher than the control single agent 360 g / L buprofezin suspension and 20% fluensulfone suspension.

[0146] Table 6: Control effect of each treatment on red spider mites of citrus on the 3rd day after treatment

[0147]

[0148] Note: The control effect (%) in the table is the average value of each repetition, and the difference is at the 5% level, and the following tables are the same.

[0149] As can be seen from Table 7, with the increase of time, the control effect of the fluensulfone and buprofezin mixed preparation on red spider mites of citrus becomes larger, and the effective period is longer. The control effects of 40% fluensulfone·buprofezin water dispersible granules (20+20) and 32% fluensulfone·buprofezin suspension (17+15) on red spider mites of citrus are 95.91% and 95.20% respectively. At the 0.05 level, through difference analysis, the fluensulfone and buprofezin mixed preparation is significantly higher than the control single agent 360 g / L buprofezin suspension and 20% fluensulfone suspension.

[0150] Table 7: Control effect of each treatment on red spider mites of citrus on the 9th day after treatment

[0151]

[0152] Note: The control effect (%) in the table is the average value of each repetition, and the difference is at the 5% level, and the following tables are the same.

[0153] Through indoor toxicity determination and tests on citrus in the field, the fluensulfone and buprofezin mixed mite-killing composition has good control effect on phytophagous mites.

[0154] The control effect of the pesticide composition or the preparation thereof prepared by the present application is significant, and the pesticide composition or the preparation thereof is superior to single agents in delaying the generation of drug resistance and prolonging the drug retention. In addition, no drug damage to crops is found in the test, which indicates that in the case of the synergistic effect of the pesticide composition or the preparation thereof in killing insects, the production cost and use cost can be reduced, and the pesticide composition or the preparation thereof is safe to crops.

[0155] Although the present application has been described in detail with particular references to illustrative embodiments thereof, it should be understood that modifications and / or improvements can be made to the present application which do not depart from the spirit and scope of the present application, and such modifications and / or improvements are intended to be included within the scope of the present application.

Claims

1. A miticidal composition characterized in that: The acaricidal composition comprises active ingredient A and active ingredient B, the active ingredient A is fluorine mite sulfide, the active ingredient B is chlorfenapyr, and the mass ratio of fluorine mite sulfide to chlorfenapyr is 1:24-25:

1.

2. The acaricidal composition according to claim 1, characterized by The mass ratio of fluorine mite sulfide to chlorfenapyr is 1:18-16:

1.

3. The acaricidal composition according to claim 1, characterized by The mass ratio of fluorine mite sulfide to chlorfenapyr is 1:24, 1:18, 1:12, 5:6, 4:3, 16:1 or 25:

1.

4. The acaricidal composition according to claim 1, characterized by: The total mass of the acaricidal composition is 100wt%, and the content of active ingredient A and active ingredient B in the acaricidal composition is 1%-85%.

5. The acaricidal composition according to claim 1, characterized by: The acaricidal composition comprises agricultural acceptable auxiliary ingredients in addition to active ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickening agents, disintegrating agents, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

6. The acaricidal composition according to claim 5, characterized in that: The acaricidal composition is prepared into an agricultural allowable preparation form, and the preparation form is wettable powder, water dispersible granule, soluble liquid, emulsifiable concentrate, water emulsion, suspension, suspoemulsion, microemulsion or dispersible oil suspension.

7. Use of the acaricidal composition according to any one of claims 1 to 6 for controlling agricultural and forest acarids, characterized in that: The harmful mites are Tetranychus cinnabarinus and Panonychus citri.

8. Use of a miticidal composition according to claim 7, characterized in that: The acaricidal composition is applied to the harmful mites or the medium where the harmful mites grow in an effective dose.

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