Bactericidal composition and application thereof

Abstract

The invention discloses a bactericidal composition and application thereof, the active ingredients of the bactericidal composition are pyraclostrobin and copper oxychloride, and the mass ratio of pyraclostrobin to copper oxychloride is (9-25): (30-58). The invention also discloses a pesticide sterilization preparation containing the sterilization composition, and the sterilization composition or the pesticide sterilization preparation has a remarkable synergistic effect on prevention and treatment of the Asian pear fire blight bacteria, can effectively prevent and treat the Asian pear fire blight bacteria, reduces the dosage of chemical agents, delays resistance, is high in safety and environment-friendly, and can be widely applied to prevention and treatment of the Asian pear fire blight bacteria. The blank of prevention and treatment of the Asian pear fire blight bacteria is filled.

Description

Technical Field

[0001] The present application belongs to the technical field of pesticide compounding agents, and specifically relates to a fungicide composition and its use. Background Art

[0002] Erwinia pyrifoliae is a very harmful pear disease. In terms of pathogen classification, it belongs to the Enterobacteriaceae family and the genus Erwinia. The bacteria enter plant tissues through wounds, stomata or other injured parts of plants. The symptoms of pear trees caused by it include leaf chlorosis, death, leaf edge dryness, fruit rot, etc. In addition, the pathogen can also cause bark ulceration, leaf mucus secretion and stem soft rot.

[0003] As a bacterium, the Asian fire blight pathogen is quite different from other pathogens due to its unique pathogenic mechanism. Specifically, the Asian fire blight pathogen mainly infects fruit trees of the Rosaceae family, such as pears and apples, with multiple damaged parts and a narrow host range. In terms of pathogenicity, the Asian fire blight pathogen mainly causes disease by producing extracellular polysaccharides to block plant ducts and secreting pectinase to decompose cell walls, causing plant tissues to soften and rot. For example, apple tree branches will show ulcer symptoms after infection. In contrast, other common pathogenic viruses such as tobacco mosaic virus rely on interfering with the normal metabolic process of plant cells, using cell substances and energy to replicate, and changing cell functions to cause disease; fungi such as powdery mildew are obligate parasites, forming mycelium and conidia on the surface of plant leaves, plundering nutrients, and hindering photosynthesis to cause disease. Due to the uniqueness of the pathogenic mechanism, the prevention and control of the Asian fire blight pathogen is more difficult. In addition, the Asian fire blight pathogen causes disease through extracellular polysaccharides and pectinase, so it is difficult to develop a drug that inhibits the production of extracellular polysaccharides and the activity of pectinase, and it is also necessary to ensure that it is harmless to the fruit trees themselves. Therefore, the selection of drugs must take into account both blocking the pathogenicity of the pathogen and ensuring the normal physiological metabolism of the fruit trees and the quality of the fruit.

[0004] Pesticide mixture refers to the use of two or more pesticide active ingredients mixed in a certain proportion and method to achieve better control effects, delay pest resistance, reduce the amount of medicine used and the number of times the medicine is applied, etc. Screening out components with synergistic effects from known agents to form a mixture is a low-cost and effective way to develop control agents for Asian fire blight. Summary of the invention

[0005] In view of this, it is necessary for the present application to provide a fungicide composition, which combines pyraclostrobin and copper oxychloride in a specific ratio to form a fungicide composition. Pyraclostrobin and copper oxychloride have a significant synergistic effect in preventing and controlling Asian fire blight pathogen, can effectively prevent and control Asian fire blight pathogen, reduce the amount of chemical agents used, delay resistance, have high safety and are environmentally friendly, thereby filling the gap in the prevention and control of Asian fire blight pathogen.

[0006] In order to achieve the above objectives, this application adopts the following technical solutions:

[0007] One aspect of the present application provides a fungicide composition, wherein the active ingredients of the fungicide composition are pyraclostrobin and copper oxychloride, and the mass concentration ratio of pyraclostrobin to copper oxychloride is (9-25): (30-58). .

[0008] Another aspect of the present application provides a pesticide fungicide formulation containing the fungicide composition described above.

[0009] Another aspect of the present application provides use of the fungicidal composition or the pesticide fungicidal formulation described above in controlling Asian amylovora.

[0010] Beneficial effects of this application:

[0011] The bactericidal composition in the present application is composed of pyraclostrobin and copper oxychloride. Pyraclostrobin can inhibit the mitochondrial respiration of the pathogen, and copper oxychloride can dissociate copper ions to destroy the structure of the pathogen. Under the ratio in the present application, the two have a significant synergistic effect on the prevention and treatment of Asian fire blight pathogens, and can effectively prevent and treat Asian fire blight pathogens. In addition, the mixed agent also has the advantages of reducing the amount of chemical agents used, delaying resistance, high safety and environmental protection.

[0012] The introduction of the fungicide composition and pesticide fungicide formulation in the present application solves the difficulty that there is currently no effective control requirement for Asian fire blight pathogen, and fills the gap in the control agent for Asian fire blight pathogen. DETAILED DESCRIPTION

[0013] The following will clearly and completely describe the implementation methods of the present application. The technical solutions in the implementation methods described below are exemplary and are only possible technical implementations of the present application, not all possible implementations. Those skilled in the art can fully combine the implementation methods of the present application to obtain other implementation methods without creative work, and these implementation methods are also within the scope of protection of the present application.

[0014] The first aspect of the present application discloses a fungicide composition, wherein the active ingredients of the fungicide composition are pyraclostrobin and copper oxychloride.

[0015] Pyraclostrobin is a highly effective, broad-spectrum, low-toxic fungicide. Its chemical name is N-[2-[1-(4-chlorophenyl)-1H-pyrazol-3-yl]oxy]methyl-N-methoxy-N-carbamic acid methyl ester, and its molecular formula is C 19 H 18 N 3 O 4 S, molecular weight is 387.8, molecular structure is as follows:

[0016]

[0017] The mechanism of action of pyraclostrobin is mainly to block mitochondrial respiration by inhibiting electron transfer between cytochrome b and c1 of pathogenic bacteria, thereby leading to cell death. This mechanism of action makes it a respiratory inhibitor, and it has a significant inhibitory effect on spore germination and mycelial growth. In addition, it also has the functions of protection, treatment and leaf penetration conduction, and can effectively prevent and control a variety of crop diseases. Although pyraclostrobin is currently widely used in a variety of plant diseases including powdery mildew, rust, downy mildew, etc., and the applied crops cover wheat, peanuts, rice, vegetables, fruit trees and other crops, there are currently no reports of its application in the prevention and control of Erwinia pyrifoliae.

[0018] Copper oxychloride, also known as copper oxychloride, basic copper chloride, chemical formula: Cu 2 (OH) 3 Copper Royal is an inorganic fungicide, mainly used to control a variety of fungal and bacterial diseases on fruit trees, vegetable gardens, field crops, medicinal plants and ornamental plants. It is not easy to develop drug resistance and is resistant to rain erosion. It is widely used in the prevention and control of diseases of crops such as citrus, litchi, and grapes. There are no reports of using copper royal for the prevention and control of Asian fire blight (Erwinia pyrifoliae).

[0019] In the present application, pyraclostrobin and copper oxychloride are compounded to form a mixed agent, which has a significant synergistic effect on the prevention and treatment of Asian fire blight pathogen (Erwinia pyrifoliae). Specifically, pyraclostrobin belongs to the methoxyacrylate fungicide class, which can inhibit mitochondrial respiration and hinder the energy production of pathogens. Copper oxychloride is an inorganic copper fungicide. Pyraclostrobin can be dissociated in water and has good systemicity and broad-spectrum antibacterial activity. It can penetrate into plant tissues, interfere with physiological processes such as bacterial cell respiration, and can inhibit the growth and reproduction of Asian fire blight pathogens. Copper oxychloride, as an inorganic copper fungicide, can combine with bacterial proteins, enzymes, etc. by releasing copper ions, destroy the bacterial cell structure and function, and can especially effectively strike at the pathogenic links related to the cell wall of Asian fire blight pathogens. After the two are combined, they can synergistically act on Asian fire blight pathogen from different sites and modes of action, accurately target its pathogenic characteristics, effectively curb the infection and spread of the pathogen on Rosaceae fruit trees such as pears and apples, and specifically respond to the particularities of Asian fire blight pathogen in host range, pathogenic mechanism and transmission mode, avoiding the problem of general fungicides being ineffective or ineffective against non-target pathogens, filling the gap in the current lack of control agents for Asian fire blight pathogen.

[0020] In the fungicidal composition of the present application, the mass ratio of pyraclostrobin to copper oxychloride is (9-25): (30:58). Preferably, the mass ratio of pyraclostrobin to copper oxychloride is 9:58, or 13:51, or 17:44, or 21:37, or 25:31. Under these ratios, pyraclostrobin and copper oxychloride show obvious synergistic effects in preventing and controlling Asian fire blight pathogen. More preferably, the mass ratio of pyraclostrobin to copper oxychloride is 17:44. Under this ratio, the synergistic effect on preventing and controlling Asian fire blight pathogen is most significant.

[0021] The second aspect of the present application discloses a pesticide fungicide formulation, which contains the fungicide composition described above.

[0022] In the pesticide bactericidal preparation, the mass content of the bactericidal composition is 10.5% to 95% based on the total mass of the pesticide bactericidal preparation. Preferably, the mass content of the bactericidal composition is 29% to 67%.

[0023] In the pesticide fungicide preparation, based on the total mass of the pesticide fungicide preparation, the mass content of pyraclostrobin is 0.5% to 45%, and the mass content of copper oxychloride is 10% to 58%; preferably, based on the total mass of the pesticide fungicide preparation, the mass content of pyraclostrobin is 4.5% to 25%, and the mass content of copper oxychloride is 15% to 58%.

[0024] It is understandable that the pesticide fungicide formulation includes, in addition to the fungicide composition described above, at least one pesticide-acceptable adjuvant, auxiliary material and / or carrier. The adjuvant, auxiliary material and / or carrier here are all conventional choices in the art and can be selected and configured accordingly according to the pesticide fungicide formulation. Those skilled in the art have such ability.

[0025] In the present application, the dosage form of the pesticide bactericidal preparation can be any dosage form in the art, and specific examples include but are not limited to at least one of granules, aqueous emulsions, suspensions, water-dispersible granules, wettable powders, etc. Preferably, the dosage form of the pesticide bactericidal preparation is a suspension. After in-depth research and practical verification, the bactericidal composition in the present application is prepared as a suspension, which shows unique advantages compared to other conventional dosage forms:

[0026] The pathogenesis of Asian fire blight is unique. It infects and spreads rapidly in plant tissues, and requires extremely high permeability and adhesion of control agents. The formulation characteristics of the suspension concentrate and the corresponding application method can just meet the above requirements. The good fluidity and dispersibility of the suspension concentrate enable it to evenly cover the plant surface during the application process, penetrate into the gaps of plant tissues, and effectively contact and inhibit the pathogens. At the same time, the ingredients in the suspension concentrate can enhance the adhesion of the agent to the plant surface, prolong the retention time of the agent on the plant surface, and continue to exert a bactericidal effect.

[0027] In addition, with pyraclostrobin and copper oxychloride as the original drug components, the suspension agent can make them work more efficiently. Specifically, the suspension agent system uses water as the dispersion medium, and the wetting agent and dispersant therein work synergistically to be tightly adsorbed on the surface of the original drug. With the help of electrostatic action and steric hindrance effect, the original drug is highly dispersed in the system, and the suspension rate is as high as 90% or more. Even if placed in a high temperature environment of 54°C for 14 days, the suspension rate is still stably maintained at more than 90%, and there will be no paste phenomenon, which can ensure the stability of product quality. In addition, the stability of the suspension agent in a low temperature environment cannot be ignored. In order to enhance its performance under low temperature conditions, antifreeze agents such as ethylene glycol need to be added. However, due to the presence of dispersants and wetting agents, a large amount of foam is easily generated during use, affecting the use effect. The applicant has found through extensive research that when ethylene glycol is selected as an antifreeze agent and xanthan gum is added at the same time, not only can the suspension maintain good dispersibility in a low temperature environment, and both the hot storage stability and the cold storage stability meet the standards, but also the foam volume can be effectively controlled below 20 mL after 1 minute of use, which can greatly improve the practicality of the pesticide formulation.

[0028] The present application discloses the use of the fungicide composition or the pesticide fungicide formulation described above in controlling Asian fire blight pathogen.

[0029] The following are specific embodiments of the present application. It should be noted that the following specific embodiments are only for illustrative purposes and do not limit the scope of the present application in any way.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0031] In addition, unless otherwise specified, methods without specific conditions or steps are conventional methods, and the reagents and materials used are all commercially available.

[0032] Indoor biological activity experiment

[0033] 1. Experimental subjects: Erwinia pyrifoliae was obtained from the indoor biological testing laboratory of Anhui Fengle Agrochemical Co., Ltd.

[0034] 2. Experimental reagents:

[0035] (1) The technical materials of pyraclostrobin (PD20181231, Jiangsu Youjia Plant Protection Co., Ltd.) and copper oxadiazine (PD20110183, Jiangxi Heyi Chemical Co., Ltd.) were purchased from the market.

[0036] (2) The mass ratios of pyraclostrobin and copper oxychloride are 9:58, 13:51, 17:44, 21:37 and 25:30 respectively.

[0037] 3. Experimental methods:

[0038] Indoor toxicity assays were carried out with reference to the agricultural industry standard of the People's Republic of China "Guidelines for Indoor Bioassay Tests of Pesticides", fungicides NY / T1156.16-2006 (Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides - Part 3 - Test for Inhibition of Bacterial Growth) and NY / T1154.7-2006 (Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 7: Determination of the Combined Action of Mixtures). Statistical analysis was performed using DPS data processing software, and the EC50, b value standard error and 95% confidence limit of each agent were calculated to evaluate the activity of the test agents.

[0039] Among them, the co-toxicity coefficient (CTC value) of the mixed agent is calculated according to the following formula:

[0040]

[0041] in,

[0042] In the formula, CTC—coefficient of co-toxicity; AT1—measured toxicity index of the mixture; TTI—theoretical toxicity index of the mixture;

[0043] S—EC50 of the standard fungicide, unit: mg / L;

[0044] M—EC50 of the mixture, unit: mg / L;

[0045] TIA—toxicity index of agent A;

[0046] PA—percentage content of agent A in the mixture, unit: %;

[0047] TIB—toxicity index of agent B;

[0048] PB—percentage content of agent B in the mixture, unit: %.

[0049] In this experiment, pyraclostrobin was used as the standard fungicide. For the co-toxicity coefficient (CTC) of the mixed preparation, CTC≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; 80<CTC<120 indicates an additive effect. The determination results are shown in Table 1.

[0050] Table 1 Toxicity determination results of each single agent and mixed agent against Erwinia amylovora

[0051]

[0052] From the results in Table 1, it can be seen that the co-toxicity coefficients of the mixed agents in treatments C - G are all significantly greater than 120, indicating that pyraclostrobin and copper oxychloride have a significant synergistic effect in controlling Erwinia amylovora. Among them, in treatment E, when the ratio of pyraclostrobin to copper oxychloride is 17:44, the synergistic effect is the most significant, and the EC50 is the lowest, indicating the highest toxicity to Erwinia amylovora.

[0053] Based on the results of the indoor bioactivity experiment, the following are the examples of preparing agents with Examples 1 - 10, Comparative Examples 1 and 2 are the single-component control examples of pyraclostrobin and copper oxychloride, and Comparative Examples 3 and 4 are the mixed agents of typical components in common copper preparations or methoxyacrylate fungicides in the field, used to verify the control effect of the mixed of two types of fungicides with good effects on common diseases of pear trees against Erwinia amylovora. In addition, in Experimental Example 1, the physicochemical properties of the suspending agents in Examples 1 - 10 were determined. In Experimental Example 2, a field efficacy test was carried out, and the treatments C - G with the same ratio were respectively prepared into suspending agents with two contents of high and low (the content of the bactericidal composition in the low content is half of that in the high content).

[0054] Suspending agent of Example 1 (pyraclostrobin: copper oxychloride = 9:58)

[0055] This embodiment discloses a suspension, which contains a fungicide composition mixed with pyraclostrobin and cuprochloridum. The specific components and content of the suspension are as follows:

[0056]

[0057]

[0058] The specific preparation method of the suspension is as follows:

[0059] According to the composition and content recorded in this embodiment, the above materials are weighed and put into a mixing kettle to stir evenly, and then an external high-speed shearing cycle is performed to ensure that there are no large particles in the kettle during grinding. After the shearing is completed, the material enters a sand mill for sand grinding, and the sand grinding is performed until the particles are more than 90 and less than 5 microns. After the sand grinding is completed, it enters a rear mixing kettle and stirs for half an hour until it is uniform, and a suspension is obtained.

[0060] Example 2 Suspension (pyraclostrobin: copper oxychloride = 9:58)

[0061] This embodiment discloses a suspension, which contains a fungicide composition mixed with pyraclostrobin and cuprochloridum. The specific components and content of the suspension are as follows:

[0062]

[0063] The specific preparation method of the suspension is shown in Example 1.

[0064] Example 3 Suspension Concentrate (Pyraclostrobin: Copper Oxide = 13:51)

[0065] This embodiment discloses a suspension, which contains a fungicide composition mixed with pyraclostrobin and cuprochloridum. The specific components and content of the suspension are as follows:

[0066]

[0067]

[0068] The specific preparation method of the suspension is as follows with reference to Example 1.

[0069] Example 4 Suspension (pyraclostrobin: copper oxychloride = 13:51)

[0070] This embodiment discloses a suspension, which contains a fungicide composition mixed with pyraclostrobin and cuprochloridum. The specific components and content of the suspension are as follows:

[0071]

[0072] The specific preparation method of the suspension is as follows with reference to Example 1.

[0073] Example 5 Suspension Concentrate (Pyraclostrobin: Copper Oxide = 17:44)

[0074] This embodiment discloses a suspension, which contains a fungicide composition mixed with pyraclostrobin and cuprochloridum. The specific components and content of the suspension are as follows:

[0075]

[0076] The specific preparation method of the suspension is as follows with reference to Example 1.

[0077] Example 6 Suspension Concentrate (Pyraclostrobin: Copper Oxide = 17:44)

[0078] This embodiment discloses a suspension, which contains a fungicide composition mixed with pyraclostrobin and cuprochloridum. The specific components and content of the suspension are as follows:

[0079]

[0080] The specific preparation method of the suspension is as follows with reference to Example 1.

[0081] Example 7 Suspension Concentrate (Pyraclostrobin: Copper Oxide = 21:37)

[0082] This embodiment discloses a suspension, which contains a fungicide composition mixed with pyraclostrobin and cuprochloridum. The specific components and content of the suspension are as follows:

[0083]

[0084] The specific preparation method of the suspension is as follows with reference to Example 1.

[0085] Example 8 Suspension Concentrate (Pyraclostrobin: Copper Oxide = 21:37)

[0086] This embodiment discloses a suspension, which contains a fungicide composition mixed with pyraclostrobin and cuprochloridum. The specific components and content of the suspension are as follows:

[0087]

[0088]

[0089] The specific preparation method of the suspension is as follows with reference to Example 1.

[0090] Example 9 Suspension Concentrate (Pyraclostrobin: Copper Oxide = 25:30)

[0091] This embodiment discloses a suspension, which contains a fungicide composition mixed with pyraclostrobin and cuprochloridum. The specific components and content of the suspension are as follows:

[0092]

[0093] The specific preparation method of the suspension is as follows with reference to Example 1.

[0094] Example 10 Suspension Concentrate (Pyraclostrobin: Copper Oxide = 25:30)

[0095] This embodiment discloses a suspension, which contains a fungicide composition mixed with pyraclostrobin and cuprochloridum. The specific components and content of the suspension are as follows:

[0096]

[0097]

[0098] The specific preparation method of the suspension is as follows with reference to Example 1.

[0099] Comparative Example 1 Suspension Concentrate (Pyraclostrobin)

[0100] This comparative example discloses a suspension agent, which is implemented in the same manner as in Example 2, except that cuprochloridum is replaced with pyraclostrobin of equal mass. Other components and contents are the same as in Example 2. The specific components and contents of the suspension agent in this comparative example are as follows:

[0101]

[0102] The specific preparation method of the suspension is as follows with reference to Example 1.

[0103] Comparative Example 2 Suspension Agent (Copper Oxide)

[0104] This comparative example discloses a suspension agent, which adopts the same implementation as Example 2, except that pyraclostrobin is replaced with an equal mass of copper oxychloride. The other components and contents are the same as those in Example 2. The specific components and contents of the suspension agent in this comparative example are as follows:

[0105]

[0106] The specific preparation method of the suspension is as follows with reference to Example 1.

[0107] Comparative Example 3 Suspension (pyraclostrobin: basic copper sulfate = 9:58)

[0108] This comparative example discloses a suspension agent, which is implemented in the same manner as in Example 2, except that copper oxychloride is replaced with basic copper sulfate of equal mass. Other components and contents are the same as in Example 2. The specific components and contents of the suspension agent in this comparative example are as follows:

[0109]

[0110] The specific preparation method of the suspension is as follows with reference to Example 1.

[0111] Comparative Example 4 Suspension Concentrate (Enoxastrobin: Copper Oxychloride = 9:58)

[0112] This comparative example discloses a suspension concentrate, which is implemented in the same manner as in Example 2, except that pyraclostrobin is replaced with an equal mass of enoxamyl. Other components and contents are the same as in Example 2. The specific components and contents of the suspension concentrate in this comparative example are as follows:

[0113]

[0114] The specific preparation method of the suspension is as follows with reference to Example 1.

[0115] Experimental Example 1 Determination of physical and chemical properties

[0116] According to the NY / T1860-2016 test guide for determination of the physicochemical properties of pesticides, the suspension rate, hot storage stability, cold storage stability, and persistent foaming of the suspension concentrates in Examples 1-10 were measured to verify the stability of the formulation. The results are shown in Table 2.

[0117] Table 2 Physical and chemical properties of the suspending agent in Examples 1-10

[0118]

[0119] It can be seen from the experimental results in Table 2 that the suspension concentrates of Examples 1-10 are qualified in terms of suspension rate, hot storage and cold storage stability, and pourability, and the sustained foaming property is ≤ 20 ml. The physical and chemical properties are excellent, and the stability and storage properties can be guaranteed.

[0120] Experimental Example 2 Field Efficacy Test

[0121] 1. Experimental location: Pear Garden Farm, Korla, Xinjiang

[0122] 2. Experimental crops: pear trees.

[0123] 3. The test target is Erwinia pyrifoliae.

[0124] 4. The test agents were all prepared by Anhui Fengle Agrochemical Co., Ltd. with reference to Examples 1-10 and Comparative Examples 1-4. Among them, the dosage of the active ingredients in Examples 1-2, 3-4, 5-6, 7-8, and 9-10 was the same, and all were produced and prepared by Anhui Fengle Agrochemical Co., Ltd.

[0125] 5. Application method and investigation

[0126] The pesticide was applied during the flowering period of the pear trees. The application method was stem and leaf spray. The efficacy was investigated 30 days after the application. The dosage and efficacy results are shown in Table 3:

[0127]

[0128] Table 3 Test dosage and efficacy

[0129] Experimental Drugs Dosage (times of solution) Prevention effect (%) Example 1 2000 times liquid 65.2％ Example 2 4000 times dilution 66.4％ Example 3 2000 times liquid 71.9％ Example 4 4000 times dilution 72.1％ Example 5 2000 times liquid 90.3％ Example 6 4000 times dilution 91.7％ Example 7 2000 times liquid 83.6％ Example 8 4000 times dilution 84.2％ Example 9 2000 times liquid 78.4％ Example 10 4000 times dilution 79.0％ Comparative Example 1 2000 times liquid 41.5％ Comparative Example 2 2000 times liquid 36.3％ Comparative Example 3 2000 times liquid 52.1％ Comparative Example 4 2000 times liquid 56.8％ Blank control Shimizu /

[0130] It can be seen from the field efficacy test in Table 3 that the control effects of the suspension concentrates of Examples 1 and 2, Examples 3 and 4, Examples 5 and 6, Examples 7 and 8, and Examples 9 and 10 are similar, and the control effect of the high-content combination is slightly better than that of the low-content combination, which indicates that under the same ratio conditions, the combinations of different contents have similar control effects on Asian fire blight. Among them, Example 6 shows the most excellent control effect, with a control effect of up to 91.7%.

[0131] The control effect of the compound fungicidal composition in the present application significantly exceeds that of two single-dose control agents and two mixed control agents. Compared with Example 2, the two mixed control examples (Comparative Example 3 and Comparative Example 4) did not exceed 60% of the control effect on Asian fire blight pathogens at higher dosages, indicating that the common combination of common copper preparations and strobilurins has poor control effect on Asian fire blight pathogens, and the fungicidal composition provided in the present application has significant advantages in controlling Asian fire blight pathogens.

[0132] In addition, actual field observations showed that the above-mentioned test agents and control agents did not cause any symptoms of phytotoxicity during the treatment of the test crop pear trees, showing good safety for the pear trees.

[0133] In general, the fungicidal composition of pyraclostrobin and copper oxychloride provided in the present application has excellent control efficacy against Asian fire blight pathogenic bacteria and good safety. As a mixed agent combination, it shows high innovation and has significant advantages in controlling Asian fire blight pathogenic bacteria. Based on this, new agents for controlling Asian fire blight pathogenic bacteria can be developed, providing a practical and novel agent solution for the field of agricultural plant protection, which is expected to be widely used and promoted in related fields.

[0134] It should be noted that the present application is not limited to the above-mentioned embodiments. The above-mentioned embodiments are only examples, and the embodiments having the same structure as the technical idea and exerting the same effect within the scope of the technical solution of the present application are all included in the technical scope of the present application. In addition, without departing from the scope of the main purpose of the present application, various modifications that can be thought of by those skilled in the art to the embodiments and other methods of combining some of the constituent elements in the embodiments are also included in the scope of the present application.

Claims

1. A bactericidal composition, characterized in that: The active ingredients of the bactericidal composition are pyraclostrobin and copper oxychloride, and the mass ratio of pyraclostrobin to copper oxychloride is (9-25):(30-58).

2. The bactericidal composition according to claim 1, characterized in that In the bactericidal composition, the mass ratio of pyraclostrobin to cuprochloridum is 9:58, or 13:51, or 17:44, or 21:37, or 25:

31.

3. The bactericidal composition according to claim 1, characterized in that The mass ratio of pyraclostrobin to copper oxychloride is 17:

44.

4. A pesticide bactericidal preparation, characterized in that: Contains the bactericidal composition according to any one of claims 1 to 3.

5. The pesticide fungicide preparation according to claim 4, characterized in that: Based on the total mass of the pesticide bactericidal preparation, the mass content of the bactericidal composition is 10.5% to 95%.

6. The pesticide fungicide preparation according to claim 5, characterized in that: Based on the total mass of the pesticide bactericidal preparation, the mass content of the bactericidal composition is 29% to 67%.

7. The pesticide fungicide preparation according to claim 4, characterized in that: Based on the total mass of the pesticide fungicide preparation, the mass content of the pyraclostrobin is 0.5% to 45%, and the mass content of the copper oxychloride is 10% to 58%; Preferably, based on the total mass of the pesticide fungicide preparation, the mass content of pyraclostrobin is 4.5% to 25%, and the mass content of cuprous oxychloride is 15% to 58%.

8. The pesticide fungicide preparation according to claim 4, characterized in that: The pesticide fungicide formulation also includes at least any one pesticide-acceptable adjuvant, auxiliary material and / or carrier.

9. The pesticide fungicide preparation according to any one of claims 4 to 8, characterized in that: The dosage form of the pesticide bactericidal preparation is a suspension.

10. Use of the fungicidal composition according to any one of claims 1 to 3 or the pesticide fungicidal preparation according to any one of claims 4 to 9 in controlling Asian amylovora.

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