Prescription food customized for pet obesity and preparation method thereof

Abstract

The present invention relates to the technical field of feeds and particularly discloses a pet obesity customized prescription food and a preparation method thereof, the pet obesity customized prescription food comprises a composite animal protein source, a structured fat system, a functional fiber compound and a metabolism regulator; through the synergistic effect of the compound animal protein source, high-biological-value protein is provided, the muscle loss problem is reduced, the functional fiber compound is combined with the metabolism regulator, lipid metabolism can be regulated in a targeted mode, and the fat rate is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of feed, and in particular relates to a prescription food customized for pet obesity and a preparation method thereof. Background Art

[0002] With the improvement of pet breeding level, pet obesity problem is becoming more and more serious and has become one of the main diseases threatening pet health.

[0003] Existing pet weight loss foods mostly use low-calorie formulas, but have the following defects: the protein source is single, and it is impossible to maintain muscle mass while controlling calories, resulting in muscle loss during the weight loss process; the fat structure design is unreasonable, the oxidative stability is poor, and it is easy to cause lipid peroxidation, which affects the shelf life of the product and has certain limitations. Summary of the invention

[0004] The purpose of the present invention is to provide a prescription food customized for pet obesity and a preparation method thereof, so as to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A prescription food customized for pet obesity and a preparation method thereof, comprising the following components by weight percentage:

[0007] Complex animal protein source 25%-38%, structured fat system 8%-12%, functional fiber complex 10%-18%, metabolic regulator 0.5%-1.5%;

[0008] The composite animal protein source is prepared by mixing enzymatically hydrolyzed ostrich tendon collagen, defatted yak lung tissue powder and Antarctic krill enzymatic hydrolyzate in a mass ratio of 2:1:1;

[0009] The Antarctic krill enzymatic hydrolysate is an active peptide extracted from Antarctic krill by enzymatic hydrolysis, rich in astaxanthin and ω-3 fatty acids, and has both antioxidant and anti-inflammatory functions; the defatted yak lung tissue powder is yak lung that has been defatted and crushed, rich in ferritin and immunoglobulin, and provides high biological value protein and is low in fat;

[0010] The structured fat system comprises beeswax-embedded yak bone marrow microparticles and sodium alginate microencapsulated cod liver oil, wherein the mass ratio of the yak bone marrow microparticles to the cod liver oil microcapsules is 3:2;

[0011] The functional fiber composite comprises pig skin collagen fibers modified with γ-aminobutyric acid, a high-amylose starch-bovine bile acid composite and nano-camel toe bone powder, with a mass ratio of (3-5):(1-2):1;

[0012] The pigskin collagen fiber modified with γ-aminobutyric acid (GABA) is obtained by chemically grafting GABA onto the surface of the collagen fiber. GABA can inhibit appetite central nervous system signals and reduce the food intake of pets. The high-amylose starch-bovine bile acid complex is a complex formed by amylose and bile acid through hydrophobic interaction, which delays starch digestion, reduces postprandial blood sugar fluctuations, and promotes fat emulsification. The nano-camel toe bone powder is obtained by ball-milling camel toe bones to a particle size of 80 nm, which improves the bioavailability of calcium and phosphorus, simulates the natural bone mineral ratio, maintains bone health and enhances satiety.

[0013] The metabolic regulator is a mixture of pig placenta extract and red deer antler stem cell lysate at an activity unit ratio of 1:0.8;

[0014] The placenta extract contains insulin-like growth factor-1 (IGF-1), which promotes protein synthesis and reduces muscle decomposition during weight loss; the stem cell lysate of the red deer antler stem cell lysate is rich in growth factors (such as VEGF and FGF), which activates the mitochondrial function of fat cells and accelerates lipid β-oxidation.

[0015] Preferably, the preparation method of the enzymatically hydrolyzed ostrich tendon collagen comprises:

[0016] (i) placing fresh ostrich tendon in phosphate buffer containing 0.1%-0.3% tea polyphenols and cleaning it at 40 kHz ultrasonic cleaning for 30 minutes;

[0017] (ii) using a complex enzyme composed of pepsin and collagenase in a mass ratio of 1:2, and performing enzymatic hydrolysis at pH 2.5-3.0 and 37° C. in two stages: in the first stage, inactivating the enzyme at 90° C. for 10 minutes after 4 hours of enzymatic hydrolysis, and in the second stage, supplementing the enzyme with 0.05%-0.1% cysteine ​​and continuing the enzymatic hydrolysis for 2 hours;

[0018] (iii) After separation by an ultrafiltration membrane with a molecular weight cutoff of 1000 Da, the mixture was blended with maltodextrin at a mass ratio of 1:0.2 to form granules.

[0019] Preferably, the preparation of the structured fat system comprises:

[0020] (a) fresh yak bone marrow is extracted and defatted by CO2 supercritical extraction, melt-mixed with beeswax at a mass ratio of 5:1, and then formed by a spray condensation granulator at an inlet air temperature of -15°C, with a particle size of 20-50 μm to obtain yak bone marrow particles;

[0021] (b) Cod liver oil and 2% sodium alginate solution were mixed in a volume ratio of 1:3, and monodisperse droplets were formed in a 0.5% CaCl2 coagulation bath using a microfluidic chip, where the channel diameter of the microfluidic chip was 200 μm. The microcapsules were coated with 0.1% chitosan and then freeze-dried to obtain cod liver oil microcapsules.

[0022] A preparation method of a prescription food customized for pet obesity as described in any one of the above, comprising the following steps:

[0023] (1) Pre-treat the composite animal protein source in a high-voltage electrostatic field of 5 - 8 kV / cm for 30 minutes;

[0024] (2) Mix nano-sized camel toe bone powder and Lactobacillus plantarum at an inoculation amount of 10 7 CFU / g, ferment under anaerobic conditions for 24 - 36 hours, and control the reducing sugar content ≤ 1.5%;

[0025] (3) Use a triple-screw extrusion puffing machine, set a 2450 MHz, 800 W microwave emitter in the die area, so that the material puffing and drying are completed synchronously, and the final moisture content ≤ 6% to obtain the prescription food.

[0026] Preferably, in the step (3), a pulsed high magnetic field is applied synchronously in the puffing section, the magnetic field intensity is 1 - 3 T, the frequency is 50 Hz, the magnetic field direction forms an angle of 45° with the material flow direction, and the action time is 2 - 5 seconds.

[0027] Preferably, in the step (3), the puffing temperature is controlled in two stages, the first stage is maintained at 80 - 90 °C for 1 - 2 minutes, and the second stage is heated to 125 - 130 °C within 10 seconds.

[0028] Compared with the prior art, the beneficial effects of the present invention are:

[0029] (1) Through the synergistic effect of the composite animal protein source, high biological value protein is provided, the problem of muscle loss is reduced, and the functional fiber complex is combined with the metabolic regulator, which can target the regulation of lipid metabolism and achieve a reduction in the fat rate.

[0030] (2) The structured fat system uses beeswax to encapsulate yak bone marrow microparticles and sodium alginate microencapsulated cod liver oil. Through the double encapsulation technology, the fat oxidation is delayed. At the same time, the pre-treatment of the high-voltage electrostatic field enhances the protein solubility, and the pulsed high magnetic field coupled with the microwave puffing technology improves the puffing efficiency and retains the active ingredients. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is the process flow chart of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0032] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0033] Embodiment 1:

[0034] See also Figure 1 As shown, a prescription food customized for pet obesity includes the following components by weight percentage:

[0035] 32% composite animal protein source, including 16% enzymatically hydrolyzed ostrich tendon collagen, 8% defatted yak lung tissue powder, and 8% Antarctic krill enzymatic hydrolyzate;

[0036] Structured fat system 10%, including beeswax-embedded yak bone marrow microparticles 6%, sodium alginate microencapsulated cod liver oil 4%;

[0037] Functional fiber complex 15%, including γ-aminobutyric acid modified pig skin collagen fiber (substitution degree 0.5) 9%, high amylose starch-bovine bile acid complex 4%, nano-camel toe bone powder (Ca / P ratio 1.7) 2%;

[0038] Metabolic regulators 1.2%, including pig placenta extract (IGF-1 activity 220IU / g) 0.7%, red deer antler stem cell lysate (CD34+ cells 6%) 0.5%.

[0039] A method for preparing a prescription diet customized for pet obesity comprises the following steps:

[0040] (1) placing the composite animal protein source in a 6.5 V / cm high voltage electrostatic field for pretreatment for 30 minutes;

[0041] (2) Nano-camel toe bone powder and Lactobacillus plantarum were inoculated at a rate of 10 7 CFU / g mixture, fermented under anaerobic conditions for 30 hours, and controlled the reducing sugar content ≤1.5%;

[0042] (3) A three-screw extruder is used, and a 2450MHz, 800W microwave transmitter is set in the die head area to achieve simultaneous expansion and drying of the material, with a final moisture content of ≤6%, to obtain the prescribed food.

[0043] Among them, ostrich tendon collagen preparation:

[0044] 1 kg of fresh ostrich tendon was ultrasonically cleaned (40 kHz, 30 min) in pH 7.4 PBS buffer containing 0.2% tea polyphenols, and then enzymatically hydrolyzed in two stages at pH 2.8 and 37°C using a mixture of pepsin (500 U / g) and collagenase (1000 U / g) in a ratio of 1:2:

[0045] The first stage: enzymatic hydrolysis for 4 h followed by inactivation at 90 °C for 10 min;

[0046] Stage 2: Add 0.08% cysteine ​​and continue enzymatic hydrolysis for 2 h;

[0047] After ultrafiltration (1000Da membrane), it was blended with maltodextrin to form granules.

[0048] Wherein, the structured fat system is prepared:

[0049] Yak bone marrow microparticles: fresh yak bone marrow was taken and CO 2 Supercritical extraction degreasing, CO 2 After supercritical degreasing (pressure 25MPa, 45℃), it is melted with beeswax (75℃), and spray condensed for granulation (inlet air temperature -15℃, outlet air temperature 5℃);

[0050] Cod liver oil microcapsules: Cod liver oil and 2% sodium alginate solution were mixed in a volume ratio of 1:3, and monodisperse microdroplets (flow rate ratio oil phase:water phase=1:3) were prepared on a microfluidic chip (200 μm channel), and then freeze-dried after 0.1% chitosan coating;

[0051] Among them, fermentation treatment:

[0052] Nano-camel toe bone powder (ball-milled particle size 80nm) and Lactobacillus plantarum (10 7 CFU / g) was fermented anaerobically at 37℃ for 30h, and the reducing sugar content dropped to 1.3%;

[0053] Among them, gradient puffing coupled with microwave drying:

[0054] A three-screw extruder was used, with the temperature of zone 1 set at 60°C, zone 2 at 85°C, and die zone at 125°C. The microwave power of the microwave transmitter was 800W, and a pulsed strong magnetic field (2T, 50Hz, 45° angle) was applied for 3 seconds to obtain the prescription food.

[0055] Example 2: Comparative experiment

[0056] The prescription food prepared in Example 1 was selected as the experimental group; a commercially available conventional weight loss food (mainly composed of chicken meal and corn protein meal) was selected as the control group 1; and a comparative formula without adding metabolic regulators and functional fibers was selected as the control group 2;

[0057] The experimental subjects were obese beagles (weight exceeded the standard by 20%-30%), 10 in each group, fed for 8 weeks

[0058] The experimental results are shown in the following table:

[0059]

[0060]

[0061] As can be seen from the above, after 8 weeks of feeding, the average weight loss rate of the obese pets (beagles) in the experimental group reached 18.2% (only 9.7% in the control group 1), the body fat rate decreased by 31.5% (only 15.8% in the control group 1), and the muscle mass retention rate was as high as 98.5%, proving that the formula can effectively prevent muscle loss while reducing fat.

[0062] Example 3: Shelf Life Test

[0063] Accelerated oxidation test (40°C, RH75%), as shown in the following table:

[0064]

[0065] From the above, it can be seen that beeswax-sodium alginate composite encapsulation improves the oxidation stability of fat.

[0066] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A prescription food customized for pet obesity, characterized in that: The following components are included by weight percentage: Complex animal protein source 25%-38%, structured fat system 8%-12%, functional fiber complex 10%-18%, metabolic regulator 0.5%-1.5%; The composite animal protein source is prepared by mixing enzymatically hydrolyzed ostrich tendon collagen, defatted yak lung tissue powder and Antarctic krill enzymatic hydrolyzate in a mass ratio of 2:1:1; The structured fat system comprises beeswax-embedded yak bone marrow microparticles and sodium alginate microencapsulated cod liver oil, wherein the mass ratio of the yak bone marrow microparticles to the cod liver oil microcapsules is 3:2; The functional fiber composite comprises pig skin collagen fibers modified with γ-aminobutyric acid, a high-amylose starch-bovine bile acid composite and nano-camel toe bone powder, with a mass ratio of (3-5):(1-2):1; The metabolic regulator is a mixture of pig placenta extract and red deer antler stem cell lysate in an activity unit ratio of 1:0.

8.

2. The prescription food customized for pet obesity according to claim 1, characterized in that: The preparation method of the enzymatic hydrolysis of ostrich tendon collagen comprises: (i) placing fresh ostrich tendon in phosphate buffer containing 0.1%-0.3% tea polyphenols and cleaning it at 40 kHz ultrasonic cleaning for 30 minutes; (ii) using a complex enzyme composed of pepsin and collagenase in a mass ratio of 1:2, and performing enzymatic hydrolysis at pH 2.5-3.0 and 37° C. in two stages: in the first stage, inactivating the enzyme at 90° C. for 10 minutes after 4 hours of enzymatic hydrolysis, and in the second stage, supplementing the enzyme with 0.05%-0.1% cysteine ​​and continuing the enzymatic hydrolysis for 2 hours; (iii) After separation by an ultrafiltration membrane with a molecular weight cutoff of 1000 Da, the mixture was blended with maltodextrin at a mass ratio of 1:0.2 to form granules.

3. The prescription food customized for pet obesity according to claim 1, characterized in that: The preparation of the structured fat system comprises: (a) fresh yak bone marrow is extracted and defatted by CO2 supercritical extraction, melt-mixed with beeswax at a mass ratio of 5:1, and then formed by a spray condensation granulator at an inlet air temperature of -15°C, with a particle size of 20-50 μm to obtain yak bone marrow particles; (b) Cod liver oil and 2% sodium alginate solution were mixed in a volume ratio of 1:3, and monodisperse droplets were formed in a 0.5% CaCl2 coagulation bath using a microfluidic chip, where the channel diameter of the microfluidic chip was 200 μm. The microcapsules were coated with 0.1% chitosan and then freeze-dried to obtain cod liver oil microcapsules.

4. A method for preparing a prescription food customized for pet obesity according to any one of claims 1 to 3, characterized in that: The following steps are involved: (1) pre-treating the composite animal protein source in a 5-8 kV / cm high voltage electrostatic field for 30 minutes; (2) Nano-camel toe bone powder and Lactobacillus plantarum were inoculated at a rate of 10 7 CFU / g mixture, fermented under anaerobic conditions for 24-36 hours, and controlled the reducing sugar content ≤1.5%; (3) A three-screw extruder is used, and a 2450MHz, 800W microwave transmitter is set in the die head area to achieve simultaneous expansion and drying of the material, with a final moisture content of ≤6%, to obtain the prescribed food.

5. The method for preparing a prescription food customized for pet obesity according to claim 4, characterized in that: In the step (3), a pulsed strong magnetic field is synchronously applied to the puffing section, with a magnetic field strength of 1-3T, a frequency of 50Hz, a magnetic field direction forming an angle of 45° with the material flow direction, and an action time of 2-5 seconds.

6. The method for preparing a prescription food customized for pet obesity according to claim 4, characterized in that: In step (3), the puffing temperature is controlled in two stages, with the first stage being maintained at 80-90°C for 1-2 minutes and the second stage being heated to 125-130°C within 10 seconds.

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