Plant-polyphenol-rich compound Chinese herbal medicine for improving egg quality and preparation and application of plant-polyphenol-rich compound Chinese herbal medicine

Abstract

The invention discloses a compound Chinese herbal medicine rich in plant polyphenol and capable of improving egg quality as well as preparation and application of the compound Chinese herbal medicine. The compound Chinese herbal medicine is prepared from the following components: rhizoma atractylodis, dendrobium officinale, dendrobium nobile, turmeric, polygonum cuspidatum, liquorice, codonopsis pilosula, astragalus membranaceus, leonurus and herba epimedii. The whole prescription improves the production performance of laying hens and the quality of eggs by tonifying middle-Jiao and Qi, invigorating spleen and drying dampness, nourishing yin and moistening dryness, and promoting blood circulation to remove blood stasis.

Description

Technical Field

[0001] The invention relates to the field of Chinese herbal medicine feed additives, and in particular to a compound Chinese herbal medicine rich in plant polyphenols for improving egg quality, and a preparation method and application thereof. Background Art

[0002] This section merely provides background information related to the present disclosure and is not necessarily prior art.

[0004] Feedable Chinese herbal medicine is the product of long-term practice of Chinese medicine theory unique to my country. Chinese herbal medicine has the advantages of rich nutrition, no drug resistance, no residue, no toxic side effects, etc., and can effectively improve the production performance and health level of animals. As an emerging green feed additive, Chinese herbal medicine conforms to the current trend of healthy consumption, pursuit of green food and return to nature with its unique functional advantages, and is one of the ideal substitutes for antibiotics. Chinese herbal medicine can be directly crushed and added to the feed, or used after extraction. However, the efficiency of direct crushing and adding is not high, the extraction method is costly and easy to pollute, and the technology of microbial fermentation and enzymolysis can fully release the active ingredients of Chinese herbal medicine, improve biological utilization, and have the advantage of low cost. In view of the problem that laying hens have severe oxidative stress, reduced production performance, egg quality and antioxidant capacity in the late egg-laying period, the present invention provides a compound Chinese herbal medicine formula rich in plant polyphenols for improving egg quality and its preparation process, so as to effectively improve the antioxidant capacity of laying hens and egg quality. Summary of the invention

[0005] Purpose of the invention: The technical problem to be solved by the present invention is to provide a compound Chinese herbal medicine rich in plant polyphenols for improving the quality of eggs, as well as its preparation and application, in view of the deficiencies in the prior art.

[0006] In order to solve the above technical problems, the present invention discloses the following technical solutions:

[0007] In a first aspect, the present invention discloses a traditional Chinese medicine composition for improving the quality of eggs.

[0008] The composition of the invention is prepared by reasonable combination and compatibility of several Chinese medicinal materials including atractylodes, dendrobium nobile, dendrobium candidum, turmeric, polygonum cuspidatum, liquorice, codonopsis pilosula, astragalus, motherwort and epimedium.

[0009] Among them, the main drugs are Astragalus and Codonopsis, the assistant drug is Epimedium, and the other Chinese herbal medicines are adjuvant drugs. Astragalus, Codonopsis, and Licorice have the effect of tonifying the middle and replenishing qi, and the assistant drug Epimedium has the effect of replenishing qi and supporting yang, which can replenish the middle and replenish qi, promote follicle development, and prolong the peak period of egg production; Dendrobium candidum and Dendrobium nobile can benefit the stomach and produce fluid, nourish yin and clear heat, making the eggshell ruddy and smooth; Turmeric and Leonurus can promote blood circulation and remove blood stasis, Atractylodes and Polygonum cuspidatum can clear heat and dry dampness, inhibit bacteria and reduce inflammation, and significantly improve the production performance of laying hens. The whole prescription improves the production performance of laying hens and the quality of eggs by tonifying the middle and replenishing qi, strengthening the spleen and drying dampness, nourishing yin and moistening dryness, and promoting blood circulation and removing blood stasis.

[0010] The mass percentage of each component is 15-25% of Atractylodes, 5-15% of Dendrobium nobile, 5-15% of Dendrobium candidum, 10-25% of Curcuma longa, 10-25% of Polygonum cuspidatum, 3-15% of Licorice, 1-10% of Codonopsis pilosula, 1-10% of Astragalus, 1-10% of Leonurus japonicus, and 1-10% of Epimedium; preferably, the mass percentage of each component is 15-25% of Atractylodes, 5-15% of Dendrobium nobile, 5-15% of Dendrobium candidum, 10-25% of Curcuma longa, 10-25% of Polygonum cuspidatum, 3-15% of Licorice, 1-10% of Codonopsis pilosula, 1-10% of Astragalus, 1-10% of Leonurus japonicus, and 1-10% of Epimedium. -15%, turmeric 10-20%, knotweed 10-20%, licorice 5-15%, codonopsis 1-10%, astragalus 1-10%, motherwort 1-10%, epimedium 1-10%; preferably, the mass percentage of each component is atractylodes 20%, dendrobium nobile 10%, dendrobium officinale 10%, turmeric 15%, knotweed 15%, licorice 10%, codonopsis 5%, astragalus 5%, motherwort 5%, epimedium 5%.

[0011] In the second aspect, the present invention provides a Chinese medicine compound for improving egg quality and a preparation method thereof. The Chinese medicine compound is prepared by fermentation technology, which is beneficial to the absorption of Chinese medicine. At the same time, probiotics also improve the intestinal environment, make nutrition easier to absorb, and improve the egg-laying performance of laying hens, which has certain advancedness.

[0012] The Chinese medicine compound is specifically prepared by the following method: mixing the Chinese medicine composition described in the first aspect with a fermentation medium containing cellulase, inoculating fermentation bacteria, and fermenting.

[0013] The preparation method of the Chinese medicine compound is as follows: mixing the Chinese medicine composition of the first aspect with a fermentation medium containing cellulase, inoculating fermentation bacteria, and fermenting.

[0014] Wherein, the mass of cellulase in the fermentation medium is 0.01%-0.1%, preferably 0.01%-0.03%.

[0015] The cellulase activity is 45-50U / mg, and the enzyme activity is defined as: 1g enzyme powder (or 1ml enzyme solution) hydrolyzes sodium carboxymethyl cellulose substrate at 50°C and pH 4.8 for 1h to produce reducing sugar equivalent to 1mg glucose, which is 1 enzyme activity unit, expressed in U / g (or U / ml).

[0016] The fermentation medium is composed of sodium chloride, glucose, peptone, magnesium sulfate and water. The content of sodium chloride in the fermentation medium is 0.2%wt-0.8%wt, the content of glucose is 2%wt-6%wt, the content of peptone is 1%wt-4%wt, and the content of magnesium sulfate is 0.1%wt-0.3%wt.

[0017] Wherein, the mass volume ratio of the traditional Chinese medicine composition to the fermentation medium is 1kg:3-7L, preferably 1kg:4-6L.

[0018] Wherein, the fermentation bacteria include Lactobacillus plantarum and Bacillus subtilis; preferably, the volume ratio of Lactobacillus plantarum to Bacillus subtilis is 1-5:1, preferably 2-4:1; preferably, the effective viable counts of Lactobacillus plantarum and Bacillus subtilis are independently selected from 0.5-1.5×10 8 CFU / mL, preferably 1×10 8 CFU / mL; Preferably, the plant lactobacillus is Lactobacillus plantarum LSJ-TY-HYB-T7, which has been deposited in the General Microbiology Center of China Microorganism Culture Collection Committee on September 4, 2020, with the deposit number CGMCCNO.20596, and the deposit address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the Bacillus subtilis is Bacillus subtilis K12; Preferably, the access amount of the fermentation bacteria is 2%-6% of the volume of the fermentation medium, preferably 3%-5%.

[0019] Wherein, the fermentation temperature is 33-39°C, preferably 35-38°C.

[0020] Wherein, the fermentation time is 68-76h, preferably 70-74h.

[0021] In some embodiments, the Chinese medicine compound is prepared by the following method:

[0022] 1) Preparation of natural plant raw materials: Purchase high-quality, natural plant raw materials for screening and remove impurities and defective products;

[0023] 2) Drying: Place the selected natural plant raw materials in an oven and dry them at 45-50°C for 4-6 hours;

[0024] 3) Crushing: Use a crusher to crush various natural plant materials in the formula separately;

[0025] 4) Sieving: The crushed natural plant material is sieved through a 60-mesh sieve;

[0026] 5) Thorough mixing: Accurately weigh the crushed natural plant raw materials according to the weight ratio specified in the formula and mix them thoroughly.

[0027] 6) Preparation of fermentation medium: prepare the fermentation medium according to the ratio of 0.5% sodium chloride, 4% glucose, 2% peptone and 0.2% magnesium sulfate, sterilize in a high pressure steam sterilizer at 121° C. for 20 min, cool to room temperature and add 0.02% cellulase.

[0028] 7) Take the fully mixed Chinese herbal medicine, add the Chinese herbal medicine and fermentation medium according to the solid-liquid ratio of 1:5, and then add 4% compound probiotic solution. After mixing, seal and ferment in a constant temperature shaker at 37°C for 72 hours.

[0029] In a third aspect, the present invention discloses the use of the Chinese medicine composition described in the first aspect and the Chinese medicine compound described in the second aspect in the preparation of products that improve the production performance of laying hens or the quality of eggs.

[0030] Wherein, the application is preferably to improve the production performance of late laying hens or the quality of egg products.

[0031] Wherein, the product comprises feed; the Chinese medicinal compound is 0.05-1% of the mass of the feed, preferably 0.1-0.5%, preferably 0.2-0.3%, such as 0.25%.

[0032] The present invention discloses a preparation and application of a fermented Chinese herbal medicine rich in plant polyphenols for improving egg quality, which is prepared by compound fermentation of multiple Chinese herbal medicines, and relates to a fermentation technology and application of multiple Chinese herbal medicine compatibility and composite probiotics. The process uses multiple Chinese herbal medicines as raw materials, and uses the plant lactobacillus LSJ-TY-HYB-T7 strain isolated from fruits and the Bacillus subtilis K12 strain for co-fermentation, and the fermented Chinese herbal medicines are dried to obtain a fermented Chinese herbal medicine preparation; the preparation is used in laying hen feed as a feed additive. The preparation is non-toxic, safe, has a strong ability to scavenge free radicals in vitro, is rich in plant polyphenols, can significantly improve the antioxidant capacity of the body's antioxidant enzyme system and non-enzyme system in vivo, improves body health, improves egg quality, and has a low addition amount in feed, and can be used as a green, safe and efficient feed additive.

[0033] Beneficial effects:

[0034] (1) The Lactobacillus plantarum LSJ-TY-HYB-T7 strain selected in the present invention was isolated from fruit by our team. Compared with other Lactobacillus plantarum, the Lactobacillus plantarum LSJ-TY-HYB-T7 strain can significantly increase the content of polyphenols when used for fermentation, and can significantly enhance the antioxidant activity of the fermentation product. We innovatively applied the Lactobacillus plantarum LSJ-TY-HYB-T7 strain to the fermentation of Chinese herbal medicines.

[0035] (2) The present invention is based on the principles of traditional Chinese medicine and poultry nutrition, and the Chinese herbal medicines are rationally combined. The main medicines are astragalus and codonopsis, the auxiliary medicine is epimedium, and the other Chinese herbal medicines are adjuvants. Astragalus, codonopsis, and liquorice have the effect of tonifying the middle and replenishing qi, and epimedium has the effect of replenishing qi and supporting yang, which can replenish the middle and replenish qi, promote follicle development, and prolong the peak period of egg laying; Dendrobium candidum and Dendrobium nobile can benefit the stomach and produce body fluid, nourish yin and clear heat, and make the eggshell ruddy and smooth; turmeric and motherwort can promote blood circulation and remove blood stasis, and atractylodes and polygonum cuspidatum can clear heat and dry dampness, inhibit bacteria and reduce inflammation, reduce the occurrence of broken eggs, soft-shell eggs, and deformed eggs, and significantly improve the production performance of laying hens. The raw materials work together to provide a fermented Chinese herbal feed additive that improves the egg-laying performance of laying hens, and can improve the antioxidant capacity and egg quality of laying hens in the later stage of egg laying. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments, and the above and / or other advantages of the present invention will become more clear.

[0037] Figure 1 The effect of the compound Chinese herbal medicine in scavenging DPPH free radicals in vitro; Note: ** indicates a significant difference compared with the control group (P < 0.01).

[0038] Figure 2 It is the content of total phenols and reducing sugars in compound Chinese herbal medicine; Note: ** indicates extremely significant difference compared with the control group (P < 0.01).

[0039] Figure 3 The in vitro DPPH free radical scavenging rate and total phenol content of Ligustrum lucidum after fermentation by different strains; Note: ** indicates extremely significant difference compared with the control group (P < 0.01).

[0040] Figure 4 The effect of the compound Chinese herbal medicine on the egg production rate of laying hens; Note: * indicates a significant difference compared with the control group (P < 0.05).

[0041] Figure 5 The antioxidant effect of the compound Chinese herbal medicine on the serum of laying hens; Note: * indicates a significant difference compared with the control group (P < 0.05). DETAILED DESCRIPTION

[0042] The present invention can be better understood according to the following examples. However, it is easy for those skilled in the art to understand that the contents described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

[0043] The experimental methods described in the following examples are conventional methods unless otherwise specified; the reagents and materials described are commercially available unless otherwise specified.

[0044] The % mentioned in the present invention, unless otherwise specified, are all mass percentages.

[0045] The cellulase activity of the present invention is 50U / mg, and the enzyme activity is defined as: 1g enzyme powder (or 1ml enzyme solution) hydrolyzes sodium carboxymethyl cellulose substrate at 50°C and pH 4.8 for 1h to produce reducing sugar equivalent to 1mg glucose, which is 1 enzyme activity unit, expressed in U / g (or U / ml).

[0046] In the following examples, the effective viable count of Lactobacillus plantarum is 1×10 8 CFU / mL, effective viable count of Bacillus subtilis is 1×10 8 CFU / mL.

[0047] Example 1: Preparation and detection of compound Chinese herbal medicine

[0048] 1. Preparation technology of compound Chinese herbal medicine

[0049] A compound Chinese herbal medicine for improving egg quality comprises the following components by weight ratio: 20% of Atractylodes lancea, 10% of Dendrobium nobile, 10% of Dendrobium candidum, 15% of Curcuma longa, 15% of Polygonum cuspidatum, 10% of Licorice, 5% of Codonopsis pilosula, 5% of Astragalus, 5% of Leonurus japonicus and 5% of Epimedium.

[0050] Its preparation method is:

[0051] 1) Preparation of natural plant raw materials: Purchase high-quality, natural, and additive-free natural plant raw materials for screening and removal of impurities and defective products;

[0052] 2) Drying: Place the selected natural plant raw materials in an oven and dry them at 45-50°C for 4-6 hours;

[0053] 3) Crushing: Use a crusher to crush various natural plant materials in the formula separately;

[0054] 4) Sieving: The crushed natural plant material is sieved through a 60-mesh sieve;

[0055] 5) Fully mixing: accurately weigh the crushed natural plant raw materials according to the weight ratio specified in the formula, and fully mix them;

[0056] 6) Preparation of fermentation medium: prepare fermentation medium (solvent is water) according to the ratio of 0.5% sodium chloride, 4% glucose, 2% peptone and 0.2% magnesium sulfate, sterilize in a high pressure steam sterilizer at 121° C. for 20 min, cool to room temperature, and add 0.02% cellulase of the total weight of the fermentation medium;

[0057] 7) taking the Chinese herbal medicine mixed in step 5), adding it to the fermentation medium prepared in step 6) at a solid-liquid ratio of 1:5 kg / L, then adding 4% vt compound probiotic solution in the fermentation medium, mixing well, sealing, and fermenting in a constant temperature shaker at 37°C for 72 hours;

[0058] Wherein, the composite probiotic bacterial liquid is a mixed bacterial liquid of Lactobacillus plantarum LSJTYHYBBT7 and Bacillus subtilis K12 in a volume ratio of 3:1.

[0059] 2. DPPH free radical scavenging ability test

[0060] The in vitro antioxidant capacity of the compound Chinese herbal medicine was determined using the Nanjing Jiancheng DPPH free radical scavenging ability kit (A153-1-1) to measure the DPPH free radical scavenging rate.

[0061] The control group is a group without adding Chinese herbal medicine, and the compound Chinese herbal medicine group is the Chinese herbal medicine in this example that has been fermented and enzymatically hydrolyzed.

[0062] like Figure 1 As shown, the in vitro experimental results showed that the compound Chinese herbal medicine had a good scavenging effect on DPPH free radicals, and the scavenging rate of DPPH free radicals reached 63.7%.

[0063] 3. Determination of total phenolic and reducing sugar content in compound Chinese herbal medicines

[0064] The total phenol content of the plant was determined by referring to the plant total phenol test kit (colorimetric method) (A143-1-1) of Nanjing Jiancheng Bioengineering Research Institute, and the soluble sugar content of the plant was determined by referring to the plant soluble sugar content test kit (colorimetric method) (BC0035) of Nanjing Jiancheng Bioengineering Research Institute.

[0065] The control group is a fermentation medium group without adding the Chinese herbal medicine group, and the compound Chinese herbal medicine group is the Chinese herbal medicine in this example that has been fermented and enzymatically hydrolyzed.

[0066] like Figure 2 As shown, the in vitro experimental results showed that the total phenol content of the compound Chinese herbal medicine reached 14.84μmol / g, and the content of reducing sugar reached 11.33mg / g.

[0067] Comparative Example 1

[0068] Same as Example 1, except that the compound Chinese herbal medicine was replaced with Ligustrum lucidum, and Ligustrum lucidum was fermented with Bacillus subtilis WB800, and a composite probiotic of Lactobacillus plantarum LSJTYHYBBT7 and Bacillus subtilis K12 in a volume ratio of 3:1. The DPPH free radical scavenging rate of Ligustrum lucidum after fermentation (Legen Bio, TO1143) and the total plant phenol content (Nanjing Jiancheng, A143-1-1) were determined.

[0069] The results are as follows Figure 3 As shown, compared with the group fermented with WB800 alone, the DPPH free radical scavenging rate of the compound probiotic fermentation group increased by 20.65%, which was a very significant difference. The total phenol content increased by 9.94 μmol / g. The fermentation effect of the compound probiotic was better than that of the fermentation with WB800 alone.

[0070] Application Example 1:

[0071] 1. Test methods

[0072] In the experiment, 144 blue-grey laying hens aged 420 days (late egg-laying period) were selected and randomly divided into a positive control group and a test group (the compound Chinese herbal medicine group fermented in Example 1), with 6 replicates in each group and 12 laying hens in each replicate.

[0073] The positive control group was fed a basal diet supplemented with 0.25%wt of antioxidant VE, and the experimental group was fed a basal diet supplemented with 0.25%wt of compound Chinese herbal medicine. The basal diet formula and nutritional level of all experimental groups were the same. The basal diet of the experimental chickens used corn, soybean meal, etc. as raw materials, and the nutritional level was based on the Chinese Chicken Feeding Standard (2004). The experimental laying hens were raised in a three-layer ladder cage, and the chickens were vaccinated and the pens were disinfected according to the routine immunization procedures.

[0074] Test method: The chickens were raised by a dedicated person and fed once a day at 7:00. The amount of feed was increased or decreased according to the amount of leftover feed so that the chickens were in a free-feeding state and free-drinking water. The combination of artificial light and natural light was used, and the light was on for 16 hours a day. The number of eggs laid by the test chickens was recorded every day. After 28 days, the chickens were slaughtered and sampled to determine the quality of the test eggs and the serum antioxidant index. Venous blood was collected from the chickens in each group and placed in a test tube without anticoagulant. The blood was naturally coagulated for 30-60 minutes at room temperature. After the blood coagulated, it was centrifuged at 3000rpm / min for 10 minutes. The supernatant was serum, which was stored in a -80℃ refrigerator. The main detection was the changes in the content of reduced glutathione (GSH) and superoxide dismutase (SOD).

[0075] 2. Test results:

[0076] 2.1 Example 1 Effect of the compound Chinese herbal medicine preparation on the egg-laying performance of laying hens

[0077] Results Figure 4 The test results show that the egg production rate of laying hens in the positive control group that has not been fermented and enzymatically treated is about 74%, while the egg production rate of laying hens in the compound Chinese herbal medicine group after fermentation and enzymatic hydrolysis by Lactobacillus plantarum LSJ-TY-HYB-T7 is about 80%, which is significantly higher than that of the control group; especially for the late egg production period, a 6% increase in egg production rate can bring great economic benefits.

[0078] 2.2 Example 1 Effect of the compound Chinese herbal medicine preparation on the antioxidant properties of laying hen serum

[0079] Results Figure 5 The experimental results showed that the compound Chinese herbal preparation can significantly increase the content of reduced glutathione GSH in the serum of laying hens (P < 0.05), and also improve the activity of superoxide dismutase SOD in the serum. Compared with the control group, the activity of serum superoxide dismutase SOD in the experimental group increased by 33.8%. The above results indicate that the compound Chinese herbal preparation can improve the antioxidant capacity of laying hens.

[0080] 2.3 Example 1 Effect of the compound Chinese herbal medicine preparation on the quality of laying eggs

[0081] Egg quality is mainly reflected by yolk color, albumen height, Haugh unit, eggshell thickness, etc. Egg weight is an important indicator for consumers to choose eggs. The heavier the egg weight, the greater the economic benefits. The albumen height can reflect the freshness of the eggs. The Haugh unit is an indicator of the freshness and protein quality of the eggs. The higher the albumen concentration, the fresher the egg and the larger the Haugh unit.

[0082] The test results are shown in Table 1. The test results show that compared with the positive control group, the addition of compound Chinese herbal preparations to laying hen diets can significantly increase egg width and egg weight (P < 0.01), and significantly improve albumen height and Haugh unit (P < 0.05). Adding fermented Chinese herbal preparations significantly increased egg weight, albumen height and Haugh unit, indicating that compound Chinese herbal preparations can improve egg quality, especially improve egg freshness, egg weight and width.

[0083] Table 1 shows the effect of compound Chinese herbal medicine on egg quality

[0084]

[0085]

[0086] Note: * indicates significant difference compared with the control group (P < 0.05), ** indicates extremely significant difference compared with the control group (P < 0.01).

[0087] Based on the above results, the compound Chinese herbal preparation has a good scavenging effect on free radicals, is rich in plant polyphenols, can improve the antioxidant properties of laying hens, and improve the quality of eggs. The compound Chinese herbal preparation can enhance the antioxidant function of laying hens by increasing the activity of antioxidant enzymes in the body, thereby ensuring the health of laying hens. Since the product is made by fermentation of a variety of Chinese herbal medicines, it is safe and reliable to use, and is green and pollution-free. It can be widely used in laying hen production.

[0088] Example 2

[0089] A compound Chinese herbal medicine for improving egg quality comprises the following components by weight ratio: 15% of Atractylodes lancea, 5% of Dendrobium nobile, 15% of Dendrobium candidum, 25% of Curcuma longa, 25% of Polygonum cuspidatum, 3% of Licorice, 3% of Codonopsis pilosula, 3% of Astragalus, 3% of Leonurus japonicus, and 1% of Epimedium. The preparation process is the same as that of Example 1.

[0090] Example 3

[0091] A compound Chinese herbal medicine for improving egg quality comprises the following components by weight ratio: 25% of Atractylodes lancea, 15% of Dendrobium nobile, 5% of Dendrobium candidum, 20% of Curcuma longa, 20% of Polygonum cuspidatum, 7% of Licorice, 2% of Codonopsis pilosula, 2% of Astragalus, 2% of Leonurus japonicus, and 2% of Epimedium. The preparation process is the same as that of Example 1.

[0092] The compound Chinese herbal medicine provided in Example 2 and Example 3 was used in a study on laying hens in the late egg-laying period with reference to Application Example 1. The results showed that the compound also had the effect of improving the production performance of laying hens and the quality of eggs.

[0093] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A Chinese medicine composition for improving egg quality, characterized in that: It is composed of the following components: Atractylodes lancea, Dendrobium officinale, Dendrobium nobile, Turmeric, Polygonum cuspidatum, Licorice, Codonopsis pilosula, Astragalus membranaceus, Leonurus japonicus and Epimedium.

2. The Chinese medicine composition according to claim 1, characterized in that: The mass percentage of each component is 15-25% of Atractylodes, 5-15% of Dendrobium nobile, 5-15% of Dendrobium candidum, 10-25% of Curcuma longa, 10-25% of Polygonum cuspidatum, 3-15% of Licorice, 1-10% of Codonopsis pilosula, 1-10% of Astragalus, 1-10% of Leonurus japonicus, and 1-10% of Epimedium; preferably, the mass percentage of each component is 15-25% of Atractylodes, 5-15% of Dendrobium nobile, 5-15% of Dendrobium candidum, 5%, turmeric 10-20%, knotweed 10-20%, liquorice 5-15%, codonopsis 1-10%, astragalus 1-10%, motherwort 1-10%, epimedium 1-10%; preferably, the mass percentage of each component is atractylodes 20%, dendrobium nobile 10%, dendrobium officinale 10%, turmeric 15%, knotweed 15%, liquorice 10%, codonopsis 5%, astragalus 5%, motherwort 5%, epimedium 5%.

3. A Chinese medicinal compound for improving egg quality, characterized in that: The method is as follows: the composition according to claim 1 or 2 is mixed with a fermentation medium containing cellulase, and fermentation bacteria are introduced and fermented.

4. A method for preparing a Chinese medicinal compound for improving egg quality, characterized in that: The method comprises the following steps: mixing the composition according to claim 1 or 2 with a fermentation medium containing cellulase, inoculating fermentation bacteria, and fermenting.

5. The Chinese medicinal compound according to claim 3 or the method according to claim 4, characterized in that: The mass of the cellulase in the fermentation medium is 0.01%-0.1%, preferably 0.01%-0.03%.

6. The Chinese medicinal compound according to claim 3 or the method according to claim 4, characterized in that: The mass volume ratio of the traditional Chinese medicine composition to the fermentation medium is 1kg:3-7L, preferably 1kg:4-6L.

7. The Chinese medicinal compound according to claim 3 or the method according to claim 4, characterized in that: The fermentation bacteria include Lactobacillus plantarum and Bacillus subtilis; preferably, the volume ratio of Lactobacillus plantarum to Bacillus subtilis is 1-5:1, preferably 2-4:1; preferably, the effective viable counts of Lactobacillus plantarum and Bacillus subtilis are independently selected from 0.5-1.5×10 8 CFU / mL, preferably 1×10 8 CFU / mL; preferably, the plant lactobacillus is Lactobacillus plantarum LSJ-TY-HYB-T7, which has been deposited in the General Microbiological Center of China National Microbiological Culture Collection Administration on September 4, 2020, with a deposit number of CGMCCNO.20596; the Bacillus subtilis is Bacillus subtilis K12; preferably, the access amount of the fermentation bacteria is 2%-6% of the volume of the fermentation medium, preferably 3%-5%.

8. The Chinese medicinal compound according to claim 3 or the method according to claim 4, characterized in that: The fermentation temperature is 33-39°C, preferably 35-38°C; the fermentation time is 68-76h, preferably 70-74h.

9. Use of the Chinese medicine composition according to claim 1 or 2 or the Chinese medicine compound according to any one of claims 3 or 5-8 in the preparation of products for improving the production performance of laying hens or the quality of eggs; preferably, for improving the production performance of late laying hens or the quality of eggs.

10. The use according to claim 9, characterized in that: The product comprises feed; the Chinese medicinal compound accounts for 0.05-1% of the mass of the feed, preferably 0.1-0.5%, preferably 0.2-0.3%.

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