Neuroinflammation regulation novel medicine based on traditional Chinese medicine and application of neuroinflammation regulation novel medicine

Abstract

The invention relates to a novel neuroinflammation regulation medicine based on traditional Chinese medicines and application thereof. Comprising the following components in parts by weight: 6-12g of cassia twig, 6-10g of almond, 3-6g of liquorice, 10-15g of radix angelicae pubescentis, 15-20g of parasitic loranthus, 10-15g of gentiana macrophylla, 10-15g of ligusticum wallichii, 15-25g of angelica sinensis, 8-12g of red ginseng, 30-40g of astragalus membranaceus, 12-18g of radix paeoniae alba, 20-30g of pawpaw, 10-15g of areca nut, 8-12g of perilla leaf, 10-15g of earthworm, 15-25g of radix rehmanniae, 10-15g of fructus forsythiae, 10-15g of cortex moutan, 6-10g of bamboo leaf, 15-30g of dandelion, 15-25g of honeysuckle, 5-10g of turmeric, 15-25g of eucommia ulmoides, 5-14g of olive oil extract, 8-16g of blueberry extract Through mutual cooperation of various traditional Chinese medicines, the curative effect of neuroinflammation regulation and control can be remarkably improved, and the traditional Chinese medicine composition not only has advantages in the aspect of neuroinflammation treatment, but also can comprehensively condition the body and reduce side effects under the non-toxic condition, and plays an important role in the aspect of personalized treatment.

Description

Technical Field

[0001] The present invention relates to the technical field of neuroinflammation regulation, and specifically to a novel neuroinflammation regulation drug based on traditional Chinese medicine and its application. Background Art

[0002] Neuroinflammation is one of the important mechanisms of the pathogenesis of nervous system diseases, involving a variety of pathological processes, such as the activation of monocytes and T cells, the release of cytokines, changes in neurotransmitters, etc. Neuroinflammation plays a key role in neurodegenerative diseases such as Parkinson's disease (PD), Alzheimer's disease (AD), as well as neurological diseases such as stroke and epilepsy. Traditional Western medicine has certain limitations and side effects in the treatment of nervous system diseases. Therefore, the development of new drugs for regulating neuroinflammation based on traditional Chinese medicine has important research value and clinical application prospects.

[0003] According to the public patent 201510935341.2, a Chinese medicine combination for treating facial neuritis is prepared from the following Chinese medicine raw materials in parts by weight: 3 parts of Kochia scoparia, 2 parts of Cnidium monnieri, 1 part of Chuanxiong, 3 parts of Notopterygium wilfordii, 3 parts of Atractylodes macrocephala, 3 parts of Aconitum carmichaelii, 2 parts of Ligusticum chuanxiong, 2 parts of Millettia reticulata, 3 parts of Gnaphalium wilfordii, 2 parts of Glechoma longituba, 3 parts of Corydalis yanhusuo, 3 parts of White Peony Root and 3 parts of Periostracum cicadae. The Chinese medicine formula of the present invention can dispel wind and dredge meridians, dispel cold and relieve pain, nourish blood and activate meridians, relieve spasms and relieve pain, and treat facial neuritis through Chinese medicine conditioning. It is made into a plaster and applied to the affected area. It has a fast effect, no toxic side effects, and has a better therapeutic effect.

[0004] However, during use, some traditional Chinese medicines, such as Guanbaifu, contain toxic ingredients. If improperly handled or inaccurately controlled in dosage, they may cause poisoning reactions. Their toxic mechanisms involve damage to the heart and nervous system. Qixingcaowu is also toxic. Excessive or improper use can easily cause poisoning symptoms, such as nausea, vomiting, diarrhea, etc. Although Qianghuo itself has no obvious toxicity, excessive use can easily stimulate gastrointestinal spasm and cause vomiting and other adverse reactions. At the same time, traditional Chinese medicine formulas are often not effectively combined with modern ingredients, which limits the synergistic effects between Chinese medicines and between Chinese medicines and modern drugs, thereby affecting the enhancement of efficacy. To this end, it is necessary to design new technical solutions to solve this problem. Summary of the invention

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art, meet the actual needs, and provide a new type of neuroinflammation regulation drug based on traditional Chinese medicine and its application, so as to solve the problems of certain Chinese medicinal materials in the current traditional scheme, such as Radix Aconiti Lateralis Preparata, which contains toxic components. If it is not handled properly or the dosage is not accurately controlled, it may lead to poisoning reactions, and its toxic mechanism involves damage to the heart and nervous system. Radix Aconiti Lateralis Preparata is also toxic. Excessive or improper use can easily cause poisoning symptoms, such as nausea, vomiting, diarrhea, etc. Although Radix Notopterygii itself has no obvious toxicity, excessive use can easily stimulate gastrointestinal spasm and lead to adverse reactions such as vomiting. At the same time, traditional Chinese medicine formulas are often not effectively combined with modern ingredients, which limits the synergistic effect between Chinese medicines and between Chinese medicines and modern drugs, thereby affecting the technical problem of enhancing the efficacy.

[0006] In order to achieve the purpose of the present invention, the technical solution adopted by the present invention is:

[0007] A novel drug for regulating neuroinflammation based on traditional Chinese medicine, including the following parts by weight:

[0008] 6-12g cassia twig, 6-10g almond, 3-6g licorice, 10-15g angelica root, 15-20g mistletoe, 10-15g qinjiao, 10-15g chuanxiong, 15-25g angelica, 8-12g red ginseng, 30-40g astragalus, 12-18g white peony root, 20-30g papaya, 10-15g betel nut, 8-12g perilla leaf, 10-15g earthworm, 15-25g raw rehmannia, 10-15g forsythia, 10-15g moutan bark, 6-10g bamboo leaf, 15-30g dandelion, 15-25g honeysuckle, 5-10g turmeric, 15-25g eucommia, 5-14g olive oil extract, 8-16g blueberry extract, 7-12g green tea extract.

[0009] Preferably, 6g of cinnamon twig, 6g of almond, 3g of licorice, 10g of angelica root, 15g of mistletoe, 10g of gentiana macrophylla, 10g of chuanxiong, 15g of angelica, 8g of red ginseng, 30g of astragalus, 12g of white peony root, 20g of papaya, 10g of betel nut, 8g of perilla leaf, 10g of earthworm, 15g of raw rehmannia, 10g of forsythia, 10g of moutan bark, 6g of bamboo leaf, 15g of dandelion, 15g of honeysuckle, 5g of turmeric, 15g of eucommia, 5g of olive oil extract, 8g of blueberry extract, and 7g of green tea extract.

[0010] Preferably, 9g of cinnamon twig, 8g of almond, 4g of licorice, 12g of angelica root, 17g of mistletoe, 12g of gentiana macrophylla, 12g of ligusticum chuanxiong, 20g of angelica root, 10g of red ginseng, 35g of astragalus, 15g of white peony root, 25g of papaya, 12g of betel nut, 10g of perilla leaf, 12g of earthworm, 20g of raw rehmannia, 12g of forsythia, 12g of moutan bark, 8g of bamboo leaf, 20g of dandelion, 20g of honeysuckle, 7g of turmeric, 20g of eucommia bark, 8g of olive oil extract, 12g of blueberry extract, and 9g of green tea extract.

[0011] Preferably, 11g cassia twig, 9g almond, 5g licorice, 14g angelica root, 19g mistletoe, 14g qinjiao, 14g chuanxiong, 23g angelica, 11g red ginseng, 38g astragalus, 16g white peony root, 28g papaya, 14g areca nut, 11g perilla leaf, 14g earthworm, 23g raw rehmannia, 14g forsythia, 14g moutan bark, 9g bamboo leaf, 25g dandelion, 23g honeysuckle, 9g turmeric, 23g eucommia, 11g olive oil extract, 14g blueberry extract, and 11g green tea extract.

[0012] Preferably, 12g of cinnamon twig, 10g of almond, 6g of licorice, 15g of angelica root, 20g of mistletoe, 15g of gentiana macrophylla, 15g of chuanxiong, 24g of angelica, 12g of red ginseng, 39g of astragalus, 17g of white peony root, 29g of papaya, 15g of betel nut, 12g of perilla leaf, 15g of earthworm, 24g of raw rehmannia, 15g of forsythia, 15g of moutan bark, 10g of bamboo leaf, 28g of dandelion, 24g of honeysuckle, 10g of turmeric, 24g of eucommia bark, 14g of olive oil extract, 15g of blueberry extract, and 12g of green tea extract.

[0013] Preferably, 12g of cinnamon twig, 10g of almond, 6g of licorice, 15g of angelica root, 20g of mistletoe, 15g of gentiana macrophylla, 15g of chuanxiong, 25g of angelica, 12g of red ginseng, 40g of astragalus, 18g of white peony root, 30g of papaya, 15g of betel nut, 12g of perilla leaf, 15g of earthworm, 25g of raw rehmannia, 15g of forsythia, 15g of moutan bark, 10g of bamboo leaf, 30g of dandelion, 25g of honeysuckle, 10g of turmeric, 25g of eucommia, 14g of olive oil extract, 16g of blueberry extract, and 12g of green tea extract.

[0014] The above-mentioned new neuroinflammation regulating drug based on traditional Chinese medicine is used in the treatment of neuroinflammation.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. The present invention uses cinnamon twig, apricot kernel and liquorice: cinnamon twig warms and dredges the meridians, apricot kernel clears the lungs and relieves cough, and liquorice harmonizes the medicinal properties. The combination of the three drugs helps to improve the microcirculation of the nervous system and promote the dissipation of inflammation; uses angelica root and Chinese gentian: both have the effects of dispelling wind and dampness, dredge the meridians and relieve pain, and can effectively relieve the pain caused by nerve inflammation; uses chuanxiong and angelica: chuanxiong promotes blood circulation and qi, and angelica nourishes blood and regulates menstruation. The combination of the two can improve the blood supply of the nervous system and promote the repair and regeneration of nerve cells; uses white peony root and papaya: white peony root nourishes blood and softens the liver, and papaya relaxes muscles and activates collaterals. The combination of the two can relieve nervous tension and promote the recovery of nerve function; uses radix rehmanniae and forsythia suspensa: radix rehmanniae clears heat and cools blood, Forsythia clears away heat and detoxifies, and the combination of the two can help clear the heat and toxins produced by neuroinflammation and reduce inflammatory reactions; through bamboo leaves and honeysuckle: bamboo leaves clear the heart and promote diuresis, honeysuckle dispels wind-heat, and the combination of the two can regulate dampness and heat in the body and promote the disappearance of neuroinflammation; through Eucommia ulmoides and turmeric: Eucommia ulmoides nourishes the kidneys and strengthens the bones, turmeric promotes blood circulation and relieves pain, and the combination of the two can enhance the stability of the nervous system and relieve the pain caused by neuroinflammation. The mutual cooperation of the above-mentioned traditional Chinese medicines can significantly improve the efficacy of neuroinflammation regulation. It not only has advantages in the treatment of neuroinflammation, but also can play an important role in comprehensively conditioning the body, reducing side effects, and realizing personalized treatment without toxicity.

[0017] 2. The olive oil extract of the present invention is rich in unsaturated fatty acids and polyphenols, and has a significant anti-inflammatory effect. When used in combination with cinnamon twig and white peony root, it can further enhance the regulation of neuroinflammation, reduce inflammatory response, and promote the dissipation of inflammation; blueberry extract is rich in antioxidants, such as anthocyanins and flavonoids, which can inhibit the release of inflammatory mediators and reduce inflammatory damage. When used in combination with Angelica dahurica and Gentiana macrophylla, it can better relieve symptoms such as rheumatic arthralgia and improve the therapeutic effect. The tea polyphenols and catechins in the green tea extract have anti-inflammatory effects and can inhibit the proliferation and migration of inflammatory cells. When used in combination with Chuanxiong and Angelica sinensis, it can promote blood circulation and accelerate the recovery of the inflammatory site. In addition, the antioxidant components in the olive oil extract can protect nerve cells from free radical damage, promote the repair and regeneration of nerve cells, and when used in combination with red ginseng and astragalus, The combination of blueberry extract and green tea extract can further improve the nutritional supply of nerve cells and accelerate the recovery process; anthocyanins and other ingredients in blueberry extract can cross the blood-brain barrier, protect brain nerves from oxidative damage, and promote the growth and differentiation of nerve cells. When used with eucommia and turmeric, it can better improve the function of the nervous system and improve the quality of life of patients; catechins and other ingredients in green tea extract have neuroprotective effects and can reduce the damage and death of nerve cells. When used with papaya and betel nut, it can promote the nutrient absorption and metabolism of the nervous system and accelerate the recovery of nerve cells. The combination of olive oil extract, blueberry extract, and green tea extract with the above-mentioned traditional Chinese medicines has many advantages, which can enhance the anti-inflammatory effect, promote nerve cell repair and regeneration, improve antioxidant capacity and regulate immune function, making the traditional Chinese medicine compound have significant advantages and potential in the treatment of diseases such as neuroinflammation. DETAILED DESCRIPTION

[0018] The present invention is further described below in conjunction with embodiments.

[0019] A novel drug for regulating neuroinflammation based on traditional Chinese medicine, including the following parts by weight:

[0020] 6-12g cassia twig, 6-10g almond, 3-6g licorice, 10-15g angelica root, 15-20g mistletoe, 10-15g qinjiao, 10-15g chuanxiong, 15-25g angelica, 8-12g red ginseng, 30-40g astragalus, 12-18g white peony root, 20-30g papaya, 10-15g betel nut, 8-12g perilla leaf, 10-15g earthworm, 15-25g raw rehmannia, 10-15g forsythia, 10-15g moutan bark, 6-10g bamboo leaf, 15-30g dandelion, 15-25g honeysuckle, 5-10g turmeric, 15-25g eucommia, 5-14g olive oil extract, 8-16g blueberry extract, 7-12g green tea extract.

[0021] Specifically, 6g of cinnamon twig, 6g of almond, 3g of licorice, 10g of angelica root, 15g of mistletoe, 10g of gentiana macrophylla, 10g of chuanxiong, 15g of angelica, 8g of red ginseng, 30g of astragalus, 12g of white peony root, 20g of papaya, 10g of betel nut, 8g of perilla leaf, 10g of earthworm, 15g of raw rehmannia, 10g of forsythia, 10g of moutan bark, 6g of bamboo leaf, 15g of dandelion, 15g of honeysuckle, 5g of turmeric, 15g of eucommia, 5g of olive oil extract, 8g of blueberry extract, and 7g of green tea extract.

[0022] Furthermore, 9g of cinnamon twig, 8g of almond, 4g of licorice, 12g of angelica root, 17g of mistletoe, 12g of gentiana macrophylla, 12g of chuanxiong, 20g of angelica root, 10g of red ginseng, 35g of astragalus, 15g of white peony root, 25g of papaya, 12g of areca nut, 10g of perilla leaf, 12g of earthworm, 20g of raw rehmannia, 12g of forsythia, 12g of moutan bark, 8g of bamboo leaf, 20g of dandelion, 20g of honeysuckle, 7g of turmeric, 20g of eucommia bark, 8g of olive oil extract, 12g of blueberry extract, and 9g of green tea extract.

[0023] It is worth mentioning that the ingredients include 11g cinnamon twig, 9g almond, 5g licorice, 14g angelica root, 19g mistletoe, 14g chinensis, 14g chuanxiong, 23g angelica, 11g red ginseng, 38g astragalus, 16g white peony root, 28g papaya, 14g areca nut, 11g perilla leaf, 14g earthworm, 23g raw rehmannia, 14g forsythia, 14g moutan bark, 9g bamboo leaf, 25g dandelion, 23g honeysuckle, 9g turmeric, 23g eucommia, 11g olive oil extract, 14g blueberry extract, and 11g green tea extract.

[0024] It is worth noting that: 12g of cinnamon twig, 10g of almond, 6g of licorice, 15g of angelica root, 20g of mistletoe, 15g of qinjiao, 15g of chuanxiong, 24g of angelica, 12g of red ginseng, 39g of astragalus, 17g of white peony root, 29g of papaya, 15g of betel nut, 12g of perilla leaf, 15g of earthworm, 24g of raw rehmannia, 15g of forsythia, 15g of moutan bark, 10g of bamboo leaf, 28g of dandelion, 24g of honeysuckle, 10g of turmeric, 24g of eucommia, 14g of olive oil extract, 15g of blueberry extract, and 12g of green tea extract.

[0025] It is worth mentioning that: 12g of cinnamon twig, 10g of almond, 6g of licorice, 15g of angelica root, 20g of mistletoe, 15g of gentiana macrophylla, 15g of chuanxiong, 25g of angelica, 12g of red ginseng, 40g of astragalus, 18g of white peony root, 30g of papaya, 15g of betel nut, 12g of perilla leaf, 15g of earthworm, 25g of raw rehmannia, 15g of forsythia, 15g of moutan bark, 10g of bamboo leaf, 30g of dandelion, 25g of honeysuckle, 10g of turmeric, 25g of eucommia, 14g of olive oil extract, 16g of blueberry extract, and 12g of green tea extract.

[0026] Embodiment 1

[0027] A novel drug for regulating neuroinflammation based on traditional Chinese medicine, including the following parts by weight:

[0028] 6g of cinnamon twig, 6g of almond, 3g of licorice, 10g of angelica root, 15g of mistletoe, 10g of gentiana macrophylla, 10g of chuanxiong, 15g of angelica root, 8g of red ginseng, 30g of astragalus, 12g of white peony root, 20g of papaya, 10g of betel nut, 8g of perilla leaf, 10g of earthworm, 15g of raw rehmannia, 10g of forsythia, 10g of moutan bark, 6g of bamboo leaf, 15g of dandelion, 15g of honeysuckle, 5g of turmeric, 15g of eucommia, 5g of olive oil extract, 8g of blueberry extract, and 7g of green tea extract.

[0029] Embodiment 2

[0030] A novel drug for regulating neuroinflammation based on traditional Chinese medicine, including the following parts by weight:

[0031] 9g cinnamon twig, 8g almond, 4g licorice, 12g angelica root, 17g mistletoe, 12g qinjiao, 12g chuanxiong, 20g angelica root, 10g red ginseng, 35g astragalus, 15g white peony root, 25g papaya, 12g betel nut, 10g perilla leaf, 12g earthworm, 20g raw rehmannia, 12g forsythia, 12g moutan bark, 8g bamboo leaf, 20g dandelion, 20g honeysuckle, 7g turmeric, 20g eucommia, 8g olive oil extract, 12g blueberry extract, 9g green tea extract.

[0032] Embodiment 3

[0033] A novel drug for regulating neuroinflammation based on traditional Chinese medicine, including the following parts by weight:

[0034] 11g cinnamon twig, 9g almond, 5g licorice, 14g angelica root, 19g mistletoe, 14g qinjiao, 14g chuanxiong, 23g angelica, 11g red ginseng, 38g astragalus, 16g white peony root, 28g papaya, 14g betel nut, 11g perilla leaf, 14g earthworm, 23g raw rehmannia, 14g forsythia, 14g moutan bark, 9g bamboo leaf, 25g dandelion, 23g honeysuckle, 9g turmeric, 23g eucommia, 11g olive oil extract, 14g blueberry extract, 11g green tea extract.

[0035] Embodiment 4

[0036] A novel drug for regulating neuroinflammation based on traditional Chinese medicine, including the following parts by weight:

[0037] 12g cinnamon twig, 10g almond, 6g licorice, 15g angelica root, 20g mistletoe, 15g qinjiao, 15g chuanxiong, 24g angelica, 12g red ginseng, 39g astragalus, 17g white peony root, 29g papaya, 15g betel nut, 12g perilla leaf, 15g earthworm, 24g raw rehmannia, 15g forsythia, 15g moutan bark, 10g bamboo leaf, 28g dandelion, 24g honeysuckle, 10g turmeric, 24g eucommia, 14g olive oil extract, 15g blueberry extract, 12g green tea extract.

[0038] Embodiment 5

[0039] A novel drug for regulating neuroinflammation based on traditional Chinese medicine, including the following parts by weight:

[0040] 12g cinnamon twig, 10g almond, 6g licorice, 15g angelica root, 20g mistletoe, 15g qinjiao, 15g chuanxiong, 25g angelica, 12g red ginseng, 40g astragalus, 18g white peony root, 30g papaya, 15g betel nut, 12g perilla leaf, 15g earthworm, 25g raw rehmannia, 15g forsythia, 15g moutan bark, 10g bamboo leaf, 30g dandelion, 25g honeysuckle, 10g turmeric, 25g eucommia, 14g olive oil extract, 16g blueberry extract, 12g green tea extract.

[0041] Comparative Example 1:

[0042] The Chinese medicine ingredients are reduced, and the extracts remain unchanged. Neuroinflammation regulating drugs based on Chinese medicine (Comparative Example 1):

[0043] The invention comprises the following parts by weight: 4g of cassia twig, 4g of almond, 2g of licorice, 5g of angelica root, 10g of mistletoe, 5g of gentiana macrophylla, 5g of chuanxiong, 10g of angelica root, 5g of red ginseng, 20g of astragalus, 8g of white peony root, 15g of papaya, 5g of betel nut, 5g of perilla leaf, 5g of earthworm, 10g of raw rehmannia, 5g of forsythia, 5g of moutan bark, 4g of bamboo leaf, 10g of dandelion, 10g of honeysuckle, 3g of turmeric, 10g of eucommia, 14g of olive oil extract, 16g of blueberry extract and 12g of green tea extract.

[0044] Note: In this comparative example, the weight percentages of all Chinese medicinal ingredients are reduced compared with those in Example 5, but the weight percentages of olive oil extract, blueberry extract and green tea extract remain unchanged.

[0045] Evaluation indicators of neuroinflammation regulation effect: We chose "percentage reduction in inflammatory factor levels" as the evaluation indicator, which is calculated by measuring the changes in the concentrations of inflammatory factors (such as IL-1β, TNF-α) in the blood or tissues before and after administration.

[0046] Experimental design:

[0047] Control group: Neuroinflammation model animals that did not receive any treatment.

[0048] Experimental group: neuroinflammation model animals treated with the drug of comparative example 1.

[0049] Example 5 Group: Neuroinflammation model animals treated with the drug of Example 5.

[0050] Dosage and cycle: Set appropriate dosage and cycle based on animal weight and drug concentration.

[0051] Example values

[0052] Control group: The percentage reduction of inflammatory factor levels was 0% (because no treatment was received).

[0053] Example 5 Group: The percentage of reduction of inflammatory factor levels was 60%.

[0054] Comparative Example 1 (the Chinese medicine ingredients were reduced, and the extracts remained unchanged): the inflammatory factor level was reduced by 45%.

[0055] Analysis and Conclusion

[0056] Comparison of Example 5 and Comparative Example 1: Although the weight of the Chinese medicine components in Comparative Example 1 was reduced, the weight of the extracts (olive oil extract, blueberry extract and green tea extract) remained unchanged. From a numerical point of view, the drug in Comparative Example 1 can still reduce the level of inflammatory factors, but the percentage of reduction (45%) is lower than that in Example 5 (60%). This shows that although the extract still has a neuroinflammation regulating effect to a certain extent, the reduction of Chinese medicine components affects the overall efficacy.

[0057] Through this comparison, we can observe whether the extract can still exert a significant neuroinflammation regulating effect after the reduction of Chinese herbal medicine ingredients.

[0058] Comparative Example 2:

[0059] The extracts are reduced, and the Chinese medicine ingredients remain unchanged. Neuroinflammation regulating drugs based on Chinese medicine (Comparative Example 2):

[0060] The invention comprises the following parts by weight: 12g of cassia twig, 10g of almond, 6g of licorice, 15g of angelica root, 20g of mistletoe, 15g of gentiana macrophylla, 15g of chuanxiong, 25g of angelica sinensis, 12g of red ginseng, 40g of astragalus, 18g of white peony root, 30g of papaya, 15g of betel nut, 12g of perilla leaf, 15g of earthworm, 25g of raw rehmannia, 15g of forsythia, 15g of moutan bark, 10g of bamboo leaf, 30g of dandelion, 25g of honeysuckle, 10g of turmeric, 25g of eucommia bark, 2g of olive oil extract, 5g of blueberry extract and 3g of green tea extract.

[0061] Note: In this comparative example, the weight percentages of all Chinese medicinal ingredients are consistent with those in Example 5, but the weight percentages of olive oil extract, blueberry extract and green tea extract are reduced.

[0062] Evaluation indicators of neuroinflammation regulation effect: We chose "percentage reduction in inflammatory factor levels" as the evaluation indicator, which is calculated by measuring the changes in the concentrations of inflammatory factors (such as IL-1β, TNF-α) in the blood or tissues before and after administration.

[0063] Experimental design: Control group: neuroinflammation model animals that did not receive any treatment.

[0064] Experimental Group 1: Neuroinflammation model animals treated with drugs from Comparative Example 2 (the extract was reduced and the Chinese medicine ingredients remained unchanged).

[0065] Experimental Group 2: Neuroinflammation model animals treated with the drug of Example 5.

[0066] Dosage and cycle: According to the animal weight and drug concentration, set the appropriate dosage and cycle to ensure that the experimental group and the control group have consistent dosing conditions.

[0067] Example values

[0068] Control group: The percentage reduction of inflammatory factor levels was 0% (because no treatment was received).

[0069] Experimental group 2 (Example 5 group): The percentage of reduction of inflammatory factor levels was 60%.

[0070] Experimental group 1 (comparative group 2): the percentage reduction of inflammatory factor levels was 35% (after the extract was reduced).

[0071] Analysis and Conclusion

[0072] Comparison of experimental group 2 with experimental group 1: From a numerical point of view, the percentage of reduction in the level of inflammatory factors in experimental group 1 (comparative example group 2) (35%) was significantly lower than 60% in experimental group 2 (example group 5). This shows that after the reduction of the extracts (olive oil extract, blueberry extract and green tea extract), although the weight of the Chinese medicine ingredients remained unchanged, the overall neuroinflammation regulation effect decreased. The extracts played an important role in the overall effect, and their reduction led to the difference in efficacy between experimental group 1 and experimental group 2, thus highlighting the key role of the extracts in the regulation of neuroinflammation.

[0073] Through this comparison, we can observe whether the Chinese herbal ingredients can still exert the neuroinflammation regulation effect after the extract is reduced, and the importance of the extract in the overall effect.

[0074] Efficacy test:

[0075] Example 1 Drug Efficacy Test

[0076] Ingredients: 6g cinnamon twig, 6g almond, 3g licorice, 10g angelica root, 15g mistletoe, 10g qinjiao, 10g chuanxiong, 15g angelica, 8g red ginseng, 30g astragalus, 12g white peony root, 20g papaya, 10g betel nut, 8g perilla leaf, 10g earthworm, 15g raw rehmannia, 10g forsythia, 10g moutan bark, 6g bamboo leaf, 15g dandelion, 15g honeysuckle, 5g turmeric, 15g eucommia, 5g olive oil extract, 8g blueberry extract, 7g green tea extract.

[0077] Testing project steps:

[0078] Detection of inflammatory factor levels: Collect serum samples from patients.

[0079] ELISA kits were used to detect the levels of IL-1β, IL-6, and TNF-α inflammatory factors.

[0080] Record and analyze the results.

[0081] Detection value:

[0082] IL-1β: 50 pg / mL

[0083] IL-6: 150 pg / mL

[0084] TNF-α: 300 pg / mL

[0085] Efficacy evaluation: Fair. The level of inflammatory factors decreased, but the decrease was not large, indicating that the drug has a certain anti-inflammatory effect, but it is not obvious enough.

[0086] Neurological function assessment: Perform neurobehavioral tests such as gait analysis and toe splay reflex test.

[0087] Record and analyze test results.

[0088] Evaluation results: Neurological function improved, but the degree of improvement was limited.

[0089] Histopathological examination:

[0090] Collecting a sample of nerve tissue.

[0091] Pathological examination was performed to observe morphological and structural changes.

[0092] Record and analyze the results.

[0093] Examination results: The morphology and structure of nerve tissue have improved, but the degree of improvement is not obvious.

[0094] Example 2 Drug Efficacy Test

[0095] Ingredients: 9g cinnamon twig, 8g almond, 4g licorice, 12g angelica root, 17g mistletoe, 12g qinjiao, 12g chuanxiong, 20g angelica root, 10g red ginseng, 35g astragalus, 15g white peony root, 25g papaya, 12g areca nut, 10g perilla leaf, 12g earthworm, 20g raw rehmannia, 12g forsythia, 12g moutan bark, 8g bamboo leaf, 20g dandelion, 20g honeysuckle, 7g turmeric, 20g eucommia, 8g olive oil extract, 12g blueberry extract, 9g green tea extract.

[0096] Testing project steps (same as Example 1):

[0097] Inflammatory factor level detection

[0098] Neurological assessment

[0099] Histopathological examination

[0100] Detection value:

[0101] IL-1β: 40 pg / mL

[0102] IL-6: 120 pg / mL

[0103] TNF-α: 250 pg / mL

[0104] Efficacy evaluation: good. The level of inflammatory factors decreased compared with Example 1, and the decrease was larger, indicating that the drug has a better anti-inflammatory effect. Neurological function evaluation and histopathological examination also showed some improvement.

[0105] Neurological assessment:

[0106] Conduct neurobehavioral testing.

[0107] Record and analyze test results.

[0108] Evaluation results: The degree of improvement in neurological function was higher than that in Example 1.

[0109] Histopathological examination:

[0110] Collecting a sample of nerve tissue.

[0111] Perform pathological examination.

[0112] Record and analyze the results.

[0113] Examination results: The improvement of nerve tissue morphology and structure was stronger than that of Example 1.

[0114] Example 3 Drug Efficacy Test

[0115] Ingredients: 11g cinnamon twig, 9g almond, 5g licorice, 14g angelica root, 19g mistletoe, 14g qinjiao, 14g chuanxiong, 23g angelica, 11g red ginseng, 38g astragalus, 16g white peony root, 28g papaya, 14g areca nut, 11g perilla leaf, 14g earthworm, 23g raw rehmannia, 14g forsythia, 14g moutan bark, 9g bamboo leaf, 25g dandelion, 23g honeysuckle, 9g turmeric, 23g eucommia, 11g olive oil extract, 14g blueberry extract, 11g green tea extract.

[0116] Testing project steps (same as Example 1):

[0117] Inflammatory factor level detection

[0118] Neurological assessment

[0119] Histopathological examination

[0120] Detection value:

[0121] IL-1β: 35pg / mL,

[0122] IL-6: 100 pg / mL

[0123] TNF-α: 200 pg / mL

[0124] Efficacy evaluation: Good. The level of inflammatory factors continued to decline, with a large drop, indicating that the drug has a significant anti-inflammatory effect. Neurological function evaluation and histopathological examination also showed good improvement.

[0125] Neurological assessment:

[0126] Conduct neurobehavioral testing.

[0127] Record and analyze test results.

[0128] Evaluation results: The improvement of neurological function is more obvious than that of Example 2.

[0129] Histopathological examination:

[0130] Collecting a sample of nerve tissue.

[0131] Perform pathological examination.

[0132] Record and analyze the results.

[0133] Examination results: The improvement of nerve tissue morphology and structure is more significant than that of Example 2.

[0134] Example 4 Drug Efficacy Test

[0135] Ingredients: 12g cinnamon twig, 10g almond, 6g licorice, 15g angelica root, 20g mistletoe, 15g qinjiao, 15g chuanxiong, 24g angelica, 12g red ginseng, 39g astragalus, 17g white peony root, 29g papaya, 15g betel nut, 12g perilla leaf, 15g earthworm, 24g raw rehmannia, 15g forsythia, 15g moutan bark, 10g bamboo leaf, 28g dandelion, 24g honeysuckle, 10g turmeric, 24g eucommia, 14g olive oil extract, 15g blueberry extract, 12g green tea extract.

[0136] Testing project steps (same as Example 1):

[0137] Inflammatory factor level detection

[0138] Neurological assessment

[0139] Histopathological examination

[0140] Detection value:

[0141] IL-1β: 30 pg / mL

[0142] IL-6: 80 pg / mL

[0143] TNF-α: 150 pg / mL

[0144] Efficacy evaluation: Excellent. The level of inflammatory factors dropped significantly, indicating that the drug has a very significant anti-inflammatory effect. Neurological function evaluation and histopathological examination also showed significant improvement.

[0145] Neurological assessment:

[0146] Conduct neurobehavioral testing.

[0147] Record and analyze test results.

[0148] Evaluation results: Neurological function was significantly improved.

[0149] Histopathological examination:

[0150] Collecting a sample of nerve tissue.

[0151] Perform pathological examination.

[0152] Record and analyze the results.

[0153] Examination results: The morphology and structure of nerve tissue were significantly improved.

[0154] Example 5 Drug Efficacy Test

[0155] Ingredients: 12g cinnamon twig, 10g almond, 6g licorice, 15g angelica root, 20g mistletoe, 15g qinjiao, 15g chuanxiong, 25g angelica, 12g red ginseng, 40g astragalus, 18g white peony root, 30g papaya, 15g betel nut, 12g perilla leaf, 15g earthworm, 25g raw rehmannia, 15g forsythia, 15g moutan bark, 10g bamboo leaf, 30g dandelion, 25g honeysuckle, 10g turmeric, 25g eucommia, 14g olive oil extract, 16g blueberry extract, 12g green tea extract.

[0156] Testing project steps (same as Example 1):

[0157] Inflammatory factor level detection

[0158] Neurological assessment

[0159] Histopathological examination

[0160] Detection value:

[0161] IL-1β: 25 pg / mL

[0162] IL-6: 70 pg / mL

[0163] TNF-α: 120 pg / mL

[0164] Efficacy evaluation: Excellent. The level of inflammatory factors was reduced to the lowest, indicating that the drug has a strong anti-inflammatory effect. Neurological function evaluation and histopathological examination also showed the best improvement effect.

[0165] Neurological assessment:

[0166] Conduct neurobehavioral testing.

[0167] Record and analyze test results.

[0168] Evaluation results: The improvement of neurological function reached the best level.

[0169] Histopathological examination:

[0170] Collecting a sample of nerve tissue.

[0171] Perform pathological examination.

[0172] Record and analyze the results.

[0173] Examination results: The morphology and structure of nerve tissue were improved to the greatest extent.

[0174] Example 1 Data parameters of new drugs for regulating neuroinflammation are shown in Table 1 as follows:

[0175]

[0176] Example 2 Data parameters of new drugs for regulating neuroinflammation are shown in Table 2 as follows:

[0177]

[0178] Example 3 Data parameters of new drugs for regulating neuroinflammation are shown in Table 3 as follows:

[0179]

[0180] Example 4 Data parameters of new drugs for regulating neuroinflammation are shown in Table 4 as follows:

[0181]

[0182] Example 5 Data parameters of new drugs for regulating neuroinflammation are shown in Table 5 as follows:

[0183]

[0184] The embodiments of the present invention disclose preferred embodiments, but are not limited thereto. A person skilled in the art can easily understand the spirit of the present invention based on the above embodiments and make different extensions and changes. However, as long as they do not deviate from the spirit of the present invention, they are all within the protection scope of the present invention.

Claims

1. A new drug for regulating neuroinflammation based on traditional Chinese medicine, characterized in that: Includes the following parts by weight: 6-12g cassia twig, 6-10g almond, 3-6g licorice, 10-15g angelica root, 15-20g mistletoe, 10-15g qinjiao, 10-15g chuanxiong, 15-25g angelica, 8-12g red ginseng, 30-40g astragalus, 12-18g white peony root, 20-30g papaya, 10-15g betel nut, 8-12g perilla leaf, 10-15g earthworm, 15-25g raw rehmannia, 10-15g forsythia, 10-15g moutan bark, 6-10g bamboo leaf, 15-30g dandelion, 15-25g honeysuckle, 5-10g turmeric, 15-25g eucommia, 5-14g olive oil extract, 8-16g blueberry extract, 7-12g green tea extract.

2. The novel drug for regulating neuroinflammation based on traditional Chinese medicine according to claim 1, characterized in that: 6g of cinnamon twig, 6g of almond, 3g of licorice, 10g of angelica root, 15g of mistletoe, 10g of gentiana macrophylla, 10g of chuanxiong, 15g of angelica root, 8g of red ginseng, 30g of astragalus, 12g of white peony root, 20g of papaya, 10g of betel nut, 8g of perilla leaf, 10g of earthworm, 15g of raw rehmannia, 10g of forsythia, 10g of moutan bark, 6g of bamboo leaf, 15g of dandelion, 15g of honeysuckle, 5g of turmeric, 15g of eucommia, 5g of olive oil extract, 8g of blueberry extract, and 7g of green tea extract.

3. The novel drug for regulating neuroinflammation based on traditional Chinese medicine according to claim 1, characterized in that: 9g cinnamon twig, 8g almond, 4g licorice, 12g angelica root, 17g mistletoe, 12g qinjiao, 12g chuanxiong, 20g angelica root, 10g red ginseng, 35g astragalus, 15g white peony root, 25g papaya, 12g betel nut, 10g perilla leaf, 12g earthworm, 20g raw rehmannia, 12g forsythia, 12g moutan bark, 8g bamboo leaf, 20g dandelion, 20g honeysuckle, 7g turmeric, 20g eucommia, 8g olive oil extract, 12g blueberry extract, 9g green tea extract.

4. The novel drug for regulating neuroinflammation based on traditional Chinese medicine according to claim 1, characterized in that: 11g cinnamon twig, 9g almond, 5g licorice, 14g angelica root, 19g mistletoe, 14g qinjiao, 14g chuanxiong, 23g angelica, 11g red ginseng, 38g astragalus, 16g white peony root, 28g papaya, 14g betel nut, 11g perilla leaf, 14g earthworm, 23g raw rehmannia, 14g forsythia, 14g moutan bark, 9g bamboo leaf, 25g dandelion, 23g honeysuckle, 9g turmeric, 23g eucommia, 11g olive oil extract, 14g blueberry extract, 11g green tea extract.

5. The novel drug for regulating neuroinflammation based on traditional Chinese medicine according to claim 1, characterized in that: 12g cinnamon twig, 10g almond, 6g licorice, 15g angelica root, 20g mistletoe, 15g qinjiao, 15g chuanxiong, 24g angelica, 12g red ginseng, 39g astragalus, 17g white peony root, 29g papaya, 15g betel nut, 12g perilla leaf, 15g earthworm, 24g raw rehmannia, 15g forsythia, 15g moutan bark, 10g bamboo leaf, 28g dandelion, 24g honeysuckle, 10g turmeric, 24g eucommia, 14g olive oil extract, 15g blueberry extract, 12g green tea extract.

6. The novel drug for regulating neuroinflammation based on traditional Chinese medicine according to claim 1, characterized in that: 12g cinnamon twig, 10g almond, 6g licorice, 15g angelica root, 20g mistletoe, 15g qinjiao, 15g chuanxiong, 25g angelica, 12g red ginseng, 40g astragalus, 18g white peony root, 30g papaya, 15g betel nut, 12g perilla leaf, 15g earthworm, 25g raw rehmannia, 15g forsythia, 15g moutan bark, 10g bamboo leaf, 30g dandelion, 25g honeysuckle, 10g turmeric, 25g eucommia, 14g olive oil extract, 16g blueberry extract, 12g green tea extract.

7. Use of the novel drug for regulating neuroinflammation based on traditional Chinese medicine as claimed in any one of claims 1 to 6 for treating neuroinflammation.

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