An amorphous alloy electrode for electrochemical glucose detection sensing

Abstract

This invention discloses an amorphous alloy electrode for electrochemical glucose detection sensing. The electrode material is an amorphous alloy with the chemical composition formula Ni. 60+x Mo 20‑x P 16 B 4， x = 0~8. The steps are as follows: First, Ni is obtained through induction melting. 60+x Mo 20‑x P 16 A B4 alloy ingot is cast, and then an amorphous alloy strip is prepared from the ingot using a single-roll spin quenching method. Finally, an electrochemical glucose detection sensing electrode is prepared by connecting copper sheets or screen-printed electrodes. This invention's amorphous alloy electrode enables the visual detection of glucose in strong alkaline or strong acid electrolyte solutions.

Description

Technical Field

[0001] This invention belongs to the field of electrochemical glucose sensing technology, and relates to an amorphous alloy electrode for electrochemical glucose detection sensing. Background Technology

[0002] With economic and social development, diabetes has become one of the most common chronic diseases in modern life. According to the latest "IDF World Diabetes Atlas (10th Edition)" released by the International Diabetes Federation (IDF) on December 6, 2021, a total of 67 million people worldwide have died from diabetes by 2021. The IDF points out that among adults aged 20-79 globally, one in ten has diabetes, and the total number of people with diabetes worldwide is as high as 537 million; furthermore, the IDF indicates that in developing countries, three in four people have diabetes. Real-time monitoring of blood glucose concentration may be an effective way to solve this problem, therefore, developing sensors that can quickly and accurately detect blood glucose levels and visualize the results is of great significance for the prevention and treatment of diabetes.

[0003] Currently, the mainstream sensor is the enzyme glucose sensor, which has significant research value in glucose detection due to its high sensitivity and selectivity. The current commercial method for human blood glucose testing is still the needle-prick blood-enzyme test strip method. This method is not only physically painful for patients but also increases the risk of infection. Furthermore, enzymes are easily deactivated by environmental factors such as pH, temperature, and humidity, and the enzyme immobilization process is very complex, with poor chemical stability. On the other hand, although some non-invasive blood glucose monitoring devices exist, existing non-invasive blood glucose meters indirectly test the concentration of glucose in the blood using methods such as infrared, electromagnetic, thermal capacity, and ultrasound. These devices are expensive and the test results have large errors. Therefore, there is an urgent need to develop a low-cost, non-invasive, rapid, accurate, and continuous glucose monitoring sensor. Summary of the Invention

[0004] The purpose of this invention is to provide an amorphous alloy electrode material for electrochemical glucose detection sensing. In a three-electrode system, it exhibits extremely high sensitivity when a glucose solution is added dropwise to an alkaline electrolyte. Simultaneously, it attempts to achieve continuous glucose monitoring by connecting a microelectrode system to a signal processing unit, providing a reference for exploring next-generation wearable, non-invasive, visual blood glucose monitoring devices.

[0005] The technical solution adopted in this invention is as follows:

[0006] An amorphous alloy electrode material for electrochemical glucose detection sensing, wherein the electrode material is an amorphous alloy with the chemical composition formula Ni. 60+xMo 20-x P 16 B 4， x = 0 to 8, preferably x = 4 to 6, and more preferably x = 4.

[0007] The preparation method of the above electrode material includes the following steps:

[0008] Step 1: Based on the chemical composition of the electrode material, alloy ingots are prepared sequentially by induction melting and arc melting methods.

[0009] Step 2: Prepare amorphous alloy strip by vacuum single-roll quenching of the obtained alloy ingot;

[0010] Step 3: Cut the obtained strip into thin sheets of the required size as electrode materials.

[0011] Preferably, in step 1, the induction melting current is 20A and the arc melting current is 160A.

[0012] Preferably, in step 2, when preparing amorphous alloy strip by vacuum single-roller spin quenching, the diameter of the quartz tube casting nozzle is 0.7-0.9 mm, with 0.8 mm being optimal.

[0013] Preferably, in step 2, when preparing amorphous alloy strip by vacuum single-roller quenching, the rotation speed of the copper roller is above 5000 rpm. If the rotation speed is too low, the cooling rate of the molten alloy liquid will be insufficient, resulting in the inability to form an amorphous alloy.

[0014] The present invention also provides an amorphous alloy electrode for electrochemical glucose detection sensing prepared by the above method. The specific steps are as follows: a conductive silver paste is applied to one surface of a clean copper sheet or the central circular area of ​​a commercial Poten-TC201 screen-printed electrode. After drying, the sheet, i.e., the electrode material, is placed on the conductive silver paste for full contact and pressing. The area other than the sheet is insulated.

[0015] The present invention also provides the use of the above-mentioned amorphous alloy electrode in the detection of glucose.

[0016] Furthermore, the glucose mentioned includes glucose from blood glucose levels.

[0017] Compared with the prior art, the present invention has the following advantages:

[0018] This invention is the first to use Ni 60+x Mo 20-x P 16An electrode made of B4 amorphous alloy strip was used to perform electrochemical sensitivity detection of glucose in 0.1M NaOH solution. Compared with conventional enzyme glucose sensors, the electrode material described in this invention has a simpler preparation process, higher stability, and is easier to store. Furthermore, it exhibits a high sensitivity of 2.503 mA / cm² in glucose electrochemical detection. -2 mM -1 Detection sensitivity. Attached Figure Description

[0019] Figure 1 Ni in Example 1 60 Mo 20 P 16 Characterization of B4 amorphous alloy electrode structure and electrochemical detection of glucose, where a is the XRD pattern, b is the glucose detection IT curve, c is the linear fitting result of the linear part in b, and the slope is the glucose detection sensitivity of the sensor.

[0020] Figure 2 Ni in Example 2 64 Mo 16 P 16 Characterization of B4 amorphous alloy electrode structure and electrochemical detection of glucose, where a is the XRD pattern, b is the glucose detection IT curve, c is the linear fitting result of the linear part in b, and the slope is the glucose detection sensitivity of the sensor.

[0021] Figure 3 Ni in Example 3 66 Mo 14 P 16 Characterization of B4 amorphous alloy electrode structure and electrochemical detection of glucose, where a is the XRD pattern, b is the glucose detection IT curve, c is the linear fitting result of the linear part in b, and the slope is the glucose detection sensitivity of the sensor.

[0022] Figure 4 Ni in Example 4 68 Mo 12 P 16 Characterization of B4 amorphous alloy electrode structure and electrochemical detection of glucose, where a is the XRD pattern, b is the glucose detection IT curve, c is the linear fitting result of the linear part in b, and the slope is the glucose detection sensitivity of the sensor.

[0023] Figure 5 Ni in Example 5 64 Mo 16 P 16 A test image of a micro-sensing system made of B4 amorphous alloy strip.

[0024] Figure 6 The Ni constructed in Example 5 64Mo 16 P 16 The glucose detection IT curve of the B4 amorphous alloy strip micro-sensor system.

[0025] Figure 7 For crystalline Ni in Comparative Example 1 64 Mo 16 P 16 The B4 alloy electrode is used for the electrochemical detection of glucose, where a is the XRD pattern, b is the glucose detection IT curve, c is the linear fitting result of the linear part in b, and the slope is the glucose detection sensitivity of the sensor. Detailed Implementation

[0026] The technical solution of the present invention will be further described below with specific embodiments. The following embodiments are all implemented under the premise of the technical solution of the present invention, and detailed implementation methods and specific operation processes are given. However, the protection scope of the present invention is not limited to the following embodiments.

[0027] Based on previous research into the compositional control of NiMo-based amorphous alloys, we have designed a fuel cell electrode material with performance comparable to Pt. According to the design principles for amorphous alloy composition, we believe that NiMoPB amorphous alloy with a suitable NiMo elemental ratio exhibits good catalytic performance in electrochemical glucose detection.

[0028] Example 1

[0029] Step 1: Accurately weigh high-purity Ni, Mo, P, and B (purity above 99.95%) according to the ingot composition ratio, and successively use induction melting and electric arc melting methods to obtain Ni. 60 Mo 20 P 16 B4 alloy ingots;

[0030] Step 2, the Ni obtained in Step 1 60 Mo 20 P 16 The B4 alloy ingot was placed in a quartz tube with a casting nozzle diameter of 0.8 mm and a vacuum was drawn.

[0031] Step 3: After the vacuum environment is formed, high-purity argon gas is introduced as a protective gas, and the rotation speed of the copper roller is set to 5000 rpm.

[0032] Step 4: Turn on the induction heating power supply and slowly increase it to 20A until Ni... 60 Mo 20 P 16 B4 alloy ingots are heated to a boiling state and held for 10 seconds;

[0033] Step 5, Ni 60 Mo 20 P16 B4 alloy molten material was sprayed and cast into amorphous alloy strips and collected;

[0034] Step 6: Coat the first surface of the clean copper sheet with conductive silver paste. After drying, place the thin film obtained in Step 5 on it for full contact and press firmly. Insulate the area other than the thin film.

[0035] Step 7: Connect the three-electrode working system (the amorphous alloy electrode prepared in this invention is the working electrode, the platinum sheet electrode is the counter electrode, and Ag / AgCl is the reference electrode) to the electrochemical workstation, and perform electrochemical tests in a 0.1M NaOH solution. Place the electrolytic cell on a magnetic stirring table with a rotation speed of 300 r / min and a temperature of 25℃.

[0036] Step 8: Perform cyclic voltammetry to activate the working electrode surface. Set the voltage window to 0.15V to 0.65V, the scan rate to 50mV / s, and the number of scan segments to 20.

[0037] Step 9: Set the timing current detection and set the potential to 0.5V. Add glucose solutions of different concentrations to the electrolytic cell so that the glucose concentration in the electrolyte solution continuously varies from 10μM to 1mM.

[0038] For Ni 60 Mo 20 P 16 XRD analysis was performed on the B4 amorphous alloy strip electrode with a scanning angle range (2θ) of 30°–60° and a scanning step size of 0.02°. The results showed a diffuse peak, distinct from the strong diffraction peaks of crystals, appearing near 2θ at approximately 43°, confirming its amorphous structure. Figure 1 As shown in 'a'.

[0039] Electrochemical sensitivity tests were performed. The current response in the IT curves under different glucose concentrations was statistically analyzed. Figure 1 As shown in b) and linear fitting, the electrochemical sensitivity of the electrode to glucose can be obtained. The results show that Ni 60 Mo 20 P 16 The B4 amorphous alloy strip electrode has a sensitivity of 0.96 mA cm⁻¹ to glucose. -2 mM, such as Figure 1 As shown in c in the figure.

[0040] Example 2

[0041] Step 1: Accurately weigh high-purity Ni, Mo, P, and B (purity above 99.95%) according to the ingot composition ratio, and successively use induction melting and electric arc melting methods to obtain Ni. 64 Mo 16 P16 B4 alloy ingots;

[0042] Step 2, the Ni obtained in Step 1 64 Mo 16 P 16 The B4 alloy ingot was placed in a quartz tube with a casting nozzle diameter of 0.8 mm and a vacuum was drawn.

[0043] Step 3: After the vacuum environment is formed, high-purity argon gas is introduced as a protective gas, and the rotation speed of the copper roller is set to 5000 rpm.

[0044] Step 4: Turn on the induction heating power supply and slowly increase it to 20A until Ni... 64 Mo 16 P 16 B4 alloy ingots are heated to a boiling state and held for 10 seconds;

[0045] Step 5, Ni 64 Mo 16 P 16 B4 alloy molten material was sprayed and cast into amorphous alloy strips and collected;

[0046] Step 6: Coat the first surface of the clean copper sheet with conductive silver paste. After drying, place the thin film obtained in Step 5 on it for full contact and press firmly. Insulate the area other than the thin film.

[0047] Step 7: Connect the three-electrode working system (the amorphous alloy electrode prepared in this invention is the working electrode, the platinum sheet electrode is the counter electrode, and Ag / AgCl is the reference electrode) to the electrochemical workstation, and perform electrochemical tests in a 0.1M NaOH solution. Place the electrolytic cell on a magnetic stirring table with a rotation speed of 300 r / min and a temperature of 25℃.

[0048] Step 8: Perform cyclic voltammetry to activate the working electrode surface. Set the voltage window to 0.15V to 0.65V, the scan rate to 50mV / s, and the number of scan segments to 20.

[0049] Step 9: Set the timing current detection and set the potential to 0.5V. Add glucose solutions of different concentrations to the electrolytic cell so that the glucose concentration in the electrolyte solution continuously varies from 10μM to 1mM.

[0050] For Ni 64 Mo 16 P 16 XRD analysis was performed on the B4 amorphous alloy strip electrode with a scanning angle range (2θ) of 30°–60° and a scanning step size of 0.02°. The results showed a diffuse peak, distinct from the strong diffraction peaks of crystals, appearing near 2θ at approximately 43°, confirming its amorphous structure. Figure 2 As shown in 'a'.

[0051] Electrochemical sensitivity tests were performed. The current response in the IT curves under different glucose concentrations was statistically analyzed. Figure 2 As shown in b) and linear fitting, the electrochemical sensitivity of the electrode to glucose can be obtained. The results show that Ni 64 Mo 16 P 16 The sensitivity of the B4 amorphous alloy strip electrode to glucose is 2.503 mA cm⁻¹. -2 mM -1 ,like Figure 2 As shown in c in the figure.

[0052] Example 3

[0053] Step 1: Accurately weigh high-purity Ni, Mo, P, and B (purity above 99.95%) according to the ingot composition ratio, and successively use induction melting and electric arc melting methods to obtain Ni. 66 Mo 14 P 16 B4 alloy ingots;

[0054] Step 2, the Ni obtained in Step 1 66 Mo 14 P 16 The B4 alloy ingot was placed in a quartz tube with a casting nozzle diameter of 0.8 mm and a vacuum was drawn.

[0055] Step 3: After the vacuum environment is formed, high-purity argon gas is introduced as a protective gas, and the rotation speed of the copper roller is set to 5000 rpm.

[0056] Step 4: Turn on the induction heating power supply and slowly increase it to 20A until Ni... 66 Mo 14 P 16 B4 alloy ingots are heated to a boiling state and held for 10 seconds;

[0057] Step 5, Ni 66 Mo 14 P 16 B4 alloy molten material was sprayed and cast into amorphous alloy strips and collected;

[0058] Step 6: Coat the first surface of the clean copper sheet with conductive silver paste. After drying, place the thin film obtained in Step 5 on it for full contact and press firmly. Insulate the area other than the thin film.

[0059] Step 7: Connect the three-electrode working system (the amorphous alloy electrode prepared in this invention is the working electrode, the platinum sheet electrode is the counter electrode, and Ag / AgCl is the reference electrode) to the electrochemical workstation, and perform electrochemical tests in a 0.1M NaOH solution. Place the electrolytic cell on a magnetic stirring table with a rotation speed of 300 r / min and a temperature of 25℃.

[0060] Step 8: Perform cyclic voltammetry to activate the working electrode surface. Set the voltage window to 0.15V to 0.65V, the scan rate to 50mV / s, and the number of scan segments to 20.

[0061] Step 9: Set the timing current detection and set the potential to 0.5V. Add glucose solutions of different concentrations to the electrolytic cell so that the glucose concentration in the electrolyte solution continuously varies from 10μM to 1mM.

[0062] For Ni 66 Mo 14 P 16 XRD analysis was performed on the B4 amorphous alloy strip electrode with a scanning angle range (2θ) of 30°–60° and a scanning step size of 0.02°. The results showed a diffuse peak, distinct from the strong diffraction peaks of crystals, appearing near 2θ at approximately 43°, confirming its amorphous structure. Figure 3 As shown in 'a'.

[0063] Electrochemical sensitivity tests were performed. The current response in the IT curves under different glucose concentrations was statistically analyzed. Figure 3 As shown in b) and linear fitting, the electrochemical sensitivity of the electrode to glucose can be obtained. The results show that Ni 66 Mo 14 P 16 The B4 amorphous alloy strip electrode has a sensitivity of 1.671 mA cm⁻¹ to glucose. -2 mM -1 ,like Figure 3 As shown in c in the figure.

[0064] Example 4

[0065] Step 1: Accurately weigh high-purity Ni, Mo, P, and B (purity above 99.95%) according to the ingot composition ratio, and successively use induction melting and electric arc melting methods to obtain Ni. 68 Mo 12 P 16 B4 alloy ingots;

[0066] Step 2, the Ni obtained in Step 1 68 Mo 12 P 16 The B4 alloy ingot was placed in a quartz tube with a casting nozzle diameter of 0.8 mm and a vacuum was drawn.

[0067] Step 3: After the vacuum environment is formed, high-purity argon gas is introduced as a protective gas, and the rotation speed of the copper roller is set to 5000 rpm.

[0068] Step 4: Turn on the induction heating power supply and slowly increase it to 20A until Ni... 68 Mo 12 P 16 B4 alloy ingots are heated to a boiling state and held for 10 seconds;

[0069] Step 5, Ni 68 Mo 12 P 16 B4 alloy molten material was sprayed and cast into amorphous alloy strips and collected;

[0070] Step 6: Coat the first surface of the clean copper sheet with conductive silver paste. After drying, place the thin film obtained in Step 5 on it for full contact and press firmly. Insulate the area other than the thin film.

[0071] Step 7: Connect the three-electrode working system (the amorphous alloy electrode prepared in this invention is the working electrode, the platinum sheet electrode is the counter electrode, and Ag / AgCl is the reference electrode) to the electrochemical workstation, and perform electrochemical tests in a 0.1M NaOH solution. Place the electrolytic cell on a magnetic stirring table with a rotation speed of 300 r / min and a temperature of 25℃.

[0072] Step 8: Perform cyclic voltammetry to activate the working electrode surface. Set the voltage window to 0.15V to 0.65V, the scan rate to 50mV / s, and the number of scan segments to 20.

[0073] Step 9: Set the timing current detection and set the potential to 0.5V. Add glucose solutions of different concentrations to the electrolytic cell so that the glucose concentration in the electrolyte solution continuously varies from 10μM to 1mM.

[0074] For Ni 68 Mo 12 P 16 XRD analysis was performed on the B4 amorphous alloy strip electrode with a scanning angle range (2θ) of 30°–60° and a scanning step size of 0.02°. The results showed a diffuse peak, distinct from the strong diffraction peaks of crystals, appearing near 2θ at approximately 43°, confirming its amorphous structure. Figure 4 As shown in 'a'.

[0075] Electrochemical sensitivity tests were performed. The current response in the IT curves under different glucose concentrations was statistically analyzed. Figure 4 As shown in b) and linear fitting, the electrochemical sensitivity of the electrode to glucose can be obtained. The results show that Ni 68 Mo12 P 16 The B4 amorphous alloy strip electrode has a sensitivity of 1.56 mA cm⁻¹ to glucose. -2 mM, such as Figure 4 As shown in c in the figure.

[0076] Example 5

[0077] Step 1: Accurately weigh high-purity Ni, Mo, P, and B (purity above 99.95%) according to the ingot composition ratio, and successively use induction melting and electric arc melting methods to obtain Ni. 64 Mo 16 P 16 B4 alloy ingots;

[0078] Step 2, take the Ni obtained in Step 1 64 Mo 16 P 16 The B4 alloy ingot was placed in a quartz tube with a casting nozzle diameter of 0.8 mm and a vacuum was drawn.

[0079] Step 3: After the vacuum environment is formed, high-purity argon gas is introduced as a protective gas, and the rotation speed of the copper roller is set to 5000 rpm.

[0080] Step 4: Turn on the induction heating power supply and slowly increase it to 20A until Ni... 64 Mo 16 P 16 B4 alloy ingots are heated to a boiling state and held for 10 seconds;

[0081] Step 5, Ni 64 Mo 16 P 16 B4 alloy molten material was sprayed and cast into amorphous alloy strips and collected;

[0082] Step 6, Ni 64 Mo 16 P 16 B4 strip is cut into 2*2mm thin sheets for later use;

[0083] Step 7: Apply conductive silver paste to the central circular area of ​​the commercial Poten-TC201 screen-printed electrode. After drying, place the thin film obtained in Step 6 on it for full contact and press firmly. Insulate the area other than the thin film.

[0084] Step 8: Connect the three-electrode working system (the amorphous alloy electrode prepared in this invention is the working electrode, the carbon electrode is the counter electrode, and the Ag / AgCl is the reference electrode) to the electrochemical workstation, and perform electrochemical tests in a 0.1M NaOH solution. Place the electrolytic cell on a magnetic stirring table with a rotation speed of 200 r / min and a temperature of 25℃.

[0085] Step 9: Perform cyclic voltammetry to activate the working electrode surface. Set the voltage window to 0.1V to 0.4V, the scan rate to 50mV / s, and the number of scan segments to 20.

[0086] Step 10: Set the timing current detection and set the potential to 0.3V. Add glucose solutions of different concentrations to the electrolytic cell so that the glucose concentration in the electrolyte solution continuously varies from 80μM to 248μM.

[0087] Figure 5 For Ni 64 Mo 16 P 16 A test image of a micro-sensing system made of B4 amorphous alloy strip.

[0088] Electrochemical tests were also performed on it. For example... Figure 6 As shown in Figure a, with the continuous change of glucose concentration in the electrolytic cell, the IT curve also shows a consistent current response. By statistically analyzing the current response in the IT curves under different glucose concentrations and performing linear fitting, the electrochemical sensitivity of the electrode to glucose can be obtained. The results show that Ni 64 Mo 16 P 16 The B4 amorphous alloy strip microsensor system has a sensitivity of 4.3678 mA cm⁻¹ for glucose. -2 mM, such as Figure 6 As shown in b in the figure. This indicates that the Ni constructed in this invention 64 Mo 16 P 16 The B4 amorphous alloy strip micro-sensing system has the capability for continuous glucose detection.

[0089] Comparative Example 1

[0090] Step 1: Accurately weigh high-purity Ni, Mo, P, and B (purity above 99.95%) according to the ingot composition ratio, and successively use induction melting and electric arc melting methods to obtain Ni. 64 Mo 16 P 16 B4 alloy ingots;

[0091] Step 2, take the Ni obtained in Step 1 64 Mo 16 P 16 The B4 alloy ingot was placed in a quartz tube with a casting nozzle diameter of 0.8 mm and a vacuum was drawn.

[0092] Step 3: After the vacuum environment is formed, high-purity argon gas is introduced as a protective gas, and the rotation speed of the copper roller is set to 4500 rpm.

[0093] Step 4: Turn on the induction heating power supply and slowly increase it to 20A until Ni... 64 Mo 16 P 16 B4 alloy ingots are heated to a boiling state and held for 10 seconds;

[0094] Step 5, Ni 64 Mo 16 P 16 B4 alloy molten liquid was sprayed and cast into crystalline alloy strips and collected;

[0095] Step 6: Coat the first surface of the clean copper sheet with conductive silver paste. After drying, place the thin film obtained in Step 5 on it for full contact and press firmly. Insulate the area other than the thin film.

[0096] Step 7: Connect the three-electrode working system (the amorphous alloy electrode prepared in this invention is the working electrode, the platinum sheet electrode is the counter electrode, and Ag / AgCl is the reference electrode) to the electrochemical workstation, and perform electrochemical tests in a 0.1M NaOH solution. Place the electrolytic cell on a magnetic stirring table with a rotation speed of 300 r / min and a temperature of 25℃.

[0097] Step 8: Perform cyclic voltammetry to activate the working electrode surface. Set the voltage window to 0.15V to 0.65V, the scan rate to 50mV / s, and the number of scan segments to 20.

[0098] Step 9: Set the timing current detection and set the potential to 0.5V. Add glucose solutions of different concentrations to the electrolytic cell so that the glucose concentration in the electrolyte solution continuously varies from 100μM to 1000μM.

[0099] XRD analysis was performed on the alloy strip with a scanning angle range (2θ) of 20°–60° and a scanning step size of 0.02°. The results showed that in addition to a diffuse peak representing an amorphous structure near 2θ of approximately 38°, strong diffraction peaks representing crystalline structures also appeared. This indicates that the strip prepared by reducing the copper roller speed underwent crystallization behavior, such as… Figure 7 As shown in 'a'.

[0100] Electrochemical tests were also performed on it. For example... Figure 7 As shown in b, the IT curve exhibits a consistent current response as the glucose concentration in the electrolytic cell continuously changes. By statistically analyzing the current responses in the IT curves under different glucose concentrations and performing linear fitting, the electrochemical sensitivity of the electrode to glucose can be obtained. The results indicate that Ni 64 Mo 16 P 16 The B4 crystalline alloy strip microsensor system has a sensitivity of 0.905 mA cm⁻¹ to glucose. -2 mM. For example, Figure 7 As shown in c in the figure. This indicates that the Ni constructed in Example 2 of this invention... 64 Mo 16 P 16 B4 amorphous alloy strip has higher glucose detection sensitivity due to the greater number of highly active unsaturated sites on the surface of its amorphous material.

[0101] In summary, Ni 64 Mo 16 P 16 The B4 amorphous alloy strip electrode exhibits ultra-high sensitivity for glucose detection, while the Ni electrode constructed in this invention... 64 Mo 16 P 16 The B4 amorphous alloy strip micro-sensing system has the capability for continuous glucose detection.

Claims

1. The use of an amorphous alloy electrode in glucose detection, wherein the amorphous alloy electrode is made of an amorphous alloy electrode material, characterized in that... The amorphous alloy electrode material is an amorphous alloy, and its chemical composition formula is Ni. 64 Mo 16 P 16 B4; Includes the following steps: Step 1: Based on the chemical composition of the amorphous alloy electrode material, alloy ingots are prepared by induction melting and arc melting in sequence, wherein the induction melting current is 20 A and the arc melting current is 160 A. Step 2: The obtained alloy ingot is used to prepare amorphous alloy strip by vacuum single-roll quenching method, with the copper roller speed being above 5000 rpm; Step 3: Cut the obtained strip into thin sheets of the required size as amorphous alloy electrode material.

2. The use as described in claim 1, characterized in that, In step 2, when preparing amorphous alloy strips by vacuum single-roller spin quenching, the diameter of the quartz tube injection nozzle is 0.7-0.9 mm.

3. The use as described in claim 1, characterized in that, In step 2, when preparing amorphous alloy strips by vacuum single-roll quenching, the diameter of the quartz tube casting nozzle is 0.8 mm.

4. The use as described in claim 1, characterized in that, The amorphous alloy electrode is made of amorphous alloy electrode material. The manufacturing steps are as follows: apply conductive silver paste to one surface of a clean copper sheet or the central circular area of ​​a commercial Poten-TC201 screen-printed electrode. After drying, place the amorphous alloy electrode material on the conductive silver paste for full contact and press firmly. The area other than the amorphous alloy electrode material is insulated.

5. The use as described in claim 1, characterized in that, The glucose mentioned includes glucose from blood glucose levels.

Images