Thrip phagostimulant and application thereof

Abstract

The invention discloses a thrip phagostimulant and application thereof, and belongs to the technical field of plant disease and pest control. The effective component of the thrip phagostimulant is at least one of L-ascorbyl stearate and L-ascorbyl palmitate, and the concentration of the effective component is 0.5%-3.0%. The attractant prepared by taking the L-ascorbyl stearate and the L-ascorbyl palmitate as effective components has a very good attraction effect on the thrips. After the two components are compounded in proportion, the attraction effect after compounding is obviously superior to that of a single component. In addition, L-ascorbyl stearate and L-ascorbyl palmitate are used as effective components of the thrip phagostimulant, and the components are natural and safe. In conclusion, the thrip phagostimulant developed by taking the L-ascorbyl stearate and the L-ascorbyl palmitate as the effective components has important application value in thrip prevention and control.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant pest control, and particularly relates to a thrips attractant and its application. Background Art

[0002] Thrips are small in size, have a short generation cycle, strong reproductive capacity, fast diffusion speed, long occurrence and damage periods, and strong concealment, making them prone to large outbreaks and difficult to control. Currently, chemical control is still the preferred measure for controlling thrips in agricultural production. However, due to their special bio-ecological characteristics and the extensive and unreasonable use of chemical agents, thrips are extremely likely to develop resistance under the selection pressure of certain insecticides.

[0003] Insects have extremely sensitive olfaction, which has important biological and ecological significance for insects to adapt to the environment and population reproduction. Utilizing this sensitive olfaction of pests to develop pest attractants has become one of the important means in the green control of pests. Plant volatile secondary metabolites have high chemical activity and play an important role in transmitting information in the interaction between plants and insects, affecting a series of behavioral responses of insects such as host location, oviposition, feeding, and attracting natural enemies of pests.

[0004] Currently, the types of plant volatile odor substances reported to have an attracting effect on thrips include benzenes, monoterpene isoprenes, phenylpropanoids, nicotinic acid, etc. In addition, anisaldehyde, benzaldehyde, and ethyl nicotinate also have good attracting effects on western flower thrips and can accurately detect the population dynamics of thrips. Besides plant volatile secondary metabolites, benzoic acid, anisaldehyde, o-anisaldehyde, geraniol, nerol, linalool, citronellol, eugenol, etc. also have a certain attracting effect on western flower thrips.

[0005] Utilizing the characteristic that thrips tend to feed on volatile substances of aromatic plants to develop natural and highly effective thrips attractants is of great significance for thrips control. Summary of the Invention

[0006] Aiming at the problems existing in the prior art, the purpose of the present invention is to provide a thrips attractant and its application.

[0007] To achieve the above purpose, the present invention adopts the following technical solutions:

[0008] A thrips attractant, the active ingredient of which is at least one of L-ascorbic acid stearate and L-ascorbic acid palmitate.

[0009] Based on the above scheme, in the thrips attractant, the concentration of the active ingredient is 0.5% - 3.0%.

[0010] Based on the above scheme, the active ingredients are L-ascorbic acid stearate and L-ascorbic acid palmitate.

[0011] On the basis of the above scheme, the mass ratio of stearyl L-ascorbate to palmitoyl L-ascorbate is 1:1.

[0012] Application of the above thrips attractant in thrips attraction.

[0013] On the basis of the above scheme, the thrips attractant is diluted 200 - 1500 times with water and sprayed on a yellow sticky board with a specification of 40 cm * 60 cm. The sticky board is fixed beside the crop to attract thrips.

[0014] On the basis of the above scheme, the dilution multiple of the thrips attractant is 500 times.

[0015] Advantages of the technical scheme of the present invention

[0016] The attractant prepared from stearyl L-ascorbate and palmitoyl L-ascorbate as active ingredients in the present invention has a good attracting effect on thrips. After the two are compounded in proportion, the attracting effect after compounding is significantly better than that of a single component. In addition, stearyl L-ascorbate and palmitoyl L-ascorbate, as active ingredients of the thrips attractant, are natural and safe in composition. In summary, the thrips attractant developed with stearyl L-ascorbate and palmitoyl L-ascorbate as active ingredients has important application value in thrips control. Description of the drawings

[0017] Figure 1 Shows the trapping effect of stearyl L-ascorbate at different concentrations on thrips;

[0018] Figure 2 Shows the trapping effect of palmitoyl L-ascorbate at different concentrations on thrips;

[0019] Figure 3 Shows the trapping effect of the compound preparation of stearyl L-ascorbate and palmitoyl L-ascorbate at different concentrations on thrips;

[0020] Figure 4 Shows the influence of the dilution multiple of the compound preparation of stearyl L-ascorbate and palmitoyl L-ascorbate on the trapping effect of thrips. Detailed implementation manners

[0021] The terms used in the present invention generally have the meanings commonly understood by those of ordinary skill in the art unless otherwise specified. The present invention will be further described in detail below with reference to specific examples and data. The following examples are only for illustrating the present invention and do not limit the scope of the present invention in any way.

[0022] In the experimental methods of the following examples, unless otherwise specified, they are all conventional methods, carried out according to the techniques or conditions described in the literature in this field or according to the product instructions. For the test materials, reagents, drugs, etc. used in the following examples, unless otherwise specified, they can all be obtained by purchasing through general channels.

[0023] The L-ascorbyl stearate in the present invention has the chemical formula C 24 H 42 O 7 , CAS No.: 25395-66-8; the chemical formula of L-ascorbyl palmitate is C 22 H 38 O 7 , CAS No.: 137-66-6.

[0024] Example 1

[0025] A thrips attractant, the active ingredient of which is L-ascorbyl stearate; its concentration is 0.5% by mass fraction.

[0026] Example 2

[0027] A thrips attractant, the active ingredient of which is L-ascorbyl stearate; its concentration is 1.0% by mass fraction.

[0028] Example 3

[0029] A thrips attractant, the active ingredient of which is L-ascorbyl stearate; its concentration is 1.5% by mass fraction.

[0030] Example 4

[0031] A thrips attractant, the active ingredient of which is L-ascorbyl stearate; its concentration is 2.0% by mass fraction.

[0032] Example 5

[0033] A thrips attractant, the active ingredient of which is L-ascorbyl stearate; its concentration is 2.5% by mass fraction.

[0034] Example 6

[0035] A thrips attractant, the active ingredient of which is L-ascorbyl stearate; its concentration is 3.0% by mass fraction.

[0036] Application of L-ascorbyl stearate in attracting field thrips

[0037] Take L-ascorbyl stearate (C 24 H 42 O 7, CAS No.: 25395-66-8), using water as the solvent, aqueous solutions with concentrations of 0.5%, 1.0%, 1.5%, 2.0%, 2.5% and 3.0% (mass fraction) were respectively prepared. When in use, 30 μL of L-ascorbyl stearate aqueous solutions with different concentrations were dissolved in 15 mL of water, and evenly sprayed on both sides of the yellow sticky traps. The specifications of the yellow sticky traps were 40 cm * 60 cm. The sticky traps were fixed beside the crops for attracting thrips.

[0038] The number of thrips attracted in 7 days was counted, and the results were as Figure 1 shown. There were significant differences in the number of thrips attracted by different concentrations of L-ascorbyl stearate solutions within 7 days. As the concentration increased, the number of thrips attracted gradually increased and became stable when the concentration reached 2.0%. The number of thrips attracted was 9.7 - 10.3. When the concentration increased further, the change in the number of thrips attracted was not significant. Considering the attracting effect and drug cost, 2.0% L-ascorbyl stearate solution was the best.

[0039] Example 7

[0040] A thrips attractant, with the active ingredient being L-ascorbyl palmitate; its concentration is 0.5% by mass fraction.

[0041] Example 8

[0042] A thrips attractant, with the active ingredient being L-ascorbyl palmitate; its concentration is 1.0% by mass fraction.

[0043] Example 9

[0044] A thrips attractant, with the active ingredient being L-ascorbyl palmitate; its concentration is 1.5% by mass fraction.

[0045] Example 10

[0046] A thrips attractant, with the active ingredient being L-ascorbyl palmitate; its concentration is 2.0% by mass fraction.

[0047] Example 11

[0048] A thrips attractant, with the active ingredient being L-ascorbyl palmitate; its concentration is 2.5% by mass fraction.

[0049] Example 12

[0050] A thrips attractant, with the active ingredient being L-ascorbyl palmitate; its concentration is 3.0% by mass fraction.

[0051] Application of L-ascorbyl palmitate in attracting field thrips

[0052] Take L-ascorbyl palmitate (C22 H 38 O 7 (CAS No.: 137 - 66 - 6), using water as the solvent, aqueous solutions with concentrations of 0.5%, 1.0%, 1.5%, 2.0%, 2.5% and 3.0% (mass fraction) were respectively prepared. When in use, 30 μL of L - ascorbyl palmitate aqueous solutions with different concentrations were dissolved in 15 mL of water and evenly sprayed on both sides of the yellow sticky traps. The yellow sticky traps had a specification of 40 cm * 60 cm. The sticky traps were fixed beside the crops for attracting thrips.

[0053] The number of thrips attracted in 7 days was counted, and the results were as Figure 2 shown. There were significant differences in the number of thrips attracted by different concentrations of L - ascorbyl palmitate solutions within 7 days. As the concentration increased, the number of thrips attracted gradually increased and became stable when the concentration reached 2.0%. The number of thrips attracted was 14.6 - 15.4. When the concentration increased further, the change in the number of thrips attracted was not significant. Considering the attracting effect and the cost of the drug, the 2.0% L - ascorbyl palmitate solution was the best.

[0054] Example 13

[0055] A thrips attractant, with the active ingredients being L - ascorbyl stearate and L - ascorbyl palmitate; among them, the concentration of L - ascorbyl stearate is 0.5% (mass fraction), and the concentration of L - ascorbyl palmitate is 0.5% (mass fraction).

[0056] Example 14

[0057] A thrips attractant, with the active ingredients being L - ascorbyl stearate and L - ascorbyl palmitate; among them, the concentration of L - ascorbyl stearate is 1.0% (mass fraction), and the concentration of L - ascorbyl palmitate is 1.0% (mass fraction).

[0058] Example 15

[0059] A thrips attractant, with the active ingredients being L - ascorbyl stearate and L - ascorbyl palmitate; among them, the concentration of L - ascorbyl stearate is 1.0% (mass fraction), and the concentration of L - ascorbyl palmitate is 2.0% (mass fraction).

[0060] Example 16

[0061] A thrips attractant, with the active ingredients being L - ascorbyl stearate and L - ascorbyl palmitate; among them, the concentration of L - ascorbyl stearate is 2.0% (mass fraction), and the concentration of L - ascorbyl palmitate is 2.0% (mass fraction).

[0062] Example 17

[0063] A thrips attractant, the active ingredients are L-ascorbyl stearate and L-ascorbyl palmitate; among them, the concentration of L-ascorbyl stearate is 3.0% (mass fraction), and the concentration of L-ascorbyl palmitate is 3.0% (mass fraction).

[0064] Application of the compound preparation of L-ascorbyl stearate and L-ascorbyl palmitate in attracting field thrips

[0065] Take L-ascorbyl stearate and L-ascorbyl palmitate, use water as a solvent, and prepare aqueous solutions with concentrations of 0.5%:0.5%, 1.0%:1.0%, 1.0%:2.0%, 2.0%:2.0%, and 3.0%:3.0% (mass fraction) respectively. When in use, take 30 μL of the compound aqueous solutions of L-ascorbyl stearate and L-ascorbyl palmitate with different concentrations and dissolve them in 15 mL of water, then evenly spray them on both the front and back sides of a yellow sticky board. The specification of the yellow sticky board is 40 cm * 60 cm. Fix the sticky board beside the crop to attract thrips.

[0066] Count the number of thrips attracted in 7 days, and the results are as Figure 3 shown. There are significant differences in the number of thrips attracted by the aqueous solutions of L-ascorbyl palmitate and L-ascorbyl palmitate with different concentrations. The number of thrips attracted by 0.5%:0.5% is in the range of 6 - 8, 1.0%:1.0% is 14 - 17, 1.0%:2.0% is 25 - 28, 2.0%:2.0% is 35 - 38, and 3.0%:3.0% is 37 - 39. The compound use of L-ascorbyl stearate and L-ascorbyl palmitate has a better attracting effect on thrips than a single component, and the best attractant effect can be obtained with a mixing ratio of 2.0%:2.0%.

[0067] Example 18

[0068] Application of thrips attractant in controlling field thrips

[0069] Take L-ascorbyl stearate and L-ascorbyl palmitate, use water as a solvent, and prepare an aqueous solution with a concentration of 2.0%:2.0% (mass fraction); dilute it 200, 300, 500, 800, 1000, and 1500 times with water respectively. 20 days after transplanting tobacco seedlings, take 15 mL of the diluted solution and evenly spray it on both the front and back sides of a yellow sticky board. The control sticky board is sprayed with clear water. The specification of the yellow sticky board is 40 cm * 60 cm. Fix the sticky board beside the tobacco seedlings. After 7 days, collect the sticky board and count the number of thrips. The plot area is 30 - 50 square meters (select 5 rows for investigating the middle three rows), with 5 replicates for each treatment, and arranged in a randomized block design.

[0070] The results are as follows Figure 4 shown. The attractant provided by the present invention has a good thrips attracting effect. Compared with the control group, the attractants diluted 200, 300, 500, 800, 1000, and 1500 times respectively attracted an average of 37, 36, 34, 28, 18, and 13 thrips, while the control group only attracted an average of 5 thrips. The attracting effects were increased by 86.1%, 85.6%, 84.9%, 81.4%, 76.6%, and 66.7% respectively. In addition, the attractants with different dilution multiples showed different attracting effects, and the number of attracted thrips decreased with the increase of the dilution multiple. In order to achieve a good thrips attracting effect and control the cost to the greatest extent, the best dilution multiple for this product as an attractant when spraying yellow sticky traps is 500 times.

[0071] As described above, it is only a preferred embodiment of the present invention, and it is not a limitation of the present invention in other forms. Any person skilled in the art may use the technical content disclosed above to make changes or modifications into equivalent embodiments with equivalent changes. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention without departing from the technical solution content of the present invention still fall within the protection scope of the technical solution of the present invention.

Claims

1. A thrips attractant, characterized in that: The active ingredient is at least one of L-ascorbyl stearate and L-ascorbyl palmitate.

2. The thrips attractant according to claim 1, characterized in that The concentration of active ingredients is 0.5% to 3.0%.

3. The thrips attractant according to claim 1, characterized in that The active ingredients are L-ascorbyl stearate and L-ascorbyl palmitate.

4. The thrips attractant according to claim 3, characterized in that: The mass ratio of L-ascorbyl stearate to L-ascorbyl palmitate is 1:

1.

5. Use of the thrips attractant according to any one of claims 1 to 4 in thrips attracting.

6. The use of the thrips attractant in thrips luring according to claim 5, characterized in that: Dilute the thrips attractant 200 to 1500 times with water, spray it on a yellow sticky insect board with a specification of 40cm*60cm, and fix the sticky insect board next to the crops to attract thrips.

7. The use of the thrips attractant in thrips luring according to claim 6, characterized in that: The dilution multiple of the thrips attractant is 500 times.

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