Sterilization composition containing Xinjunan and epoxiconazole and application thereof

Abstract

The invention discloses a sterilization composition containing xinjunan and epoxiconazole and application of a preparation. The mass ratio of the xinjunan to the epoxiconazole in the sterilization composition is 1: 9-9: 1. Compared with single use of the two components, the bactericide composition disclosed by the invention has high biological activity, delays the generation and development of drug resistance of pathogens to a greater extent, reduces the risk that pathogenic fungi generate resistance to a single bactericide, and has the characteristics of economy, high efficiency, safety and environmental protection.

Description

Technical Field

[0001] The present invention relates to the field of agricultural fungicides, and specifically to a bactericidal composition containing octhilinone and epoxiconazole for controlling wheat scab. Background Art

[0002] Wheat scab caused by Fusarium graminearum is widely distributed in global wheat-growing areas and is more severe in regions with mild climate and high humidity. Wheat scab can occur from the seedling stage to the ear stage of wheat, and is prone to outbreak and epidemic when continuous rainy weather occurs during the heading and flowering stage. It not only causes a significant reduction in wheat yield but also produces toxins, affecting the health of humans and livestock. However, the long-term and single use of common fungicides for controlling wheat scab has led to serious resistance of wheat scab pathogens to some fungicides, greatly reducing the control effect. Therefore, how to effectively control wheat scab, especially the management of resistant strains, has become a major challenge faced by agricultural researchers and growers.

[0003] Octhilinone is an alkyl polyamine fungicide that inhibits the growth and reproduction of fungi by interfering with the biosynthesis of lipids in the fungal cell membrane and destroying the structure and function of the cell membrane. This fungicide is widely used in global agricultural production, mainly for controlling plant diseases caused by various pathogenic bacteria and viruses, such as bacterial leaf streak of rice, bacterial blight of rice, bacterial angular leaf spot of cucumber, and tomato virus disease. Octhilinone not only has a significant effect on disease control but also has the characteristics of low toxicity and low residue, with less impact on the environment and non-target organisms, meeting the requirements of the green development of modern agriculture.

[0004] Epoxiconazole is a highly effective triazole fungicide that inhibits the growth and reproduction of fungi by inhibiting the biosynthesis of ergosterol in the fungal cell membrane and destroying the structure and function of the cell membrane. Epoxiconazole has both systemic and protective effects, can be absorbed through the leaves and translocated within the plant to provide long-term disease protection, and is widely used in global agricultural production, mainly for controlling various diseases on cereals such as wheat, barley, corn, and rice, as well as fruits such as grapes, apples, and pears, such as powdery mildew, rust, and leaf spot.

[0005] Both validamycin and epoxiconazole can play an antibacterial role when used alone. However, using the same pesticide alone for a long time in disease control will increase the risk of resistance in the field. The Fungicide Resistance Action Committee (FRAC) recommends that combining pesticides with different modes of action can avoid or delay the emergence of resistance. Therefore, combining the two into a bactericidal composition can not only effectively solve the practical production problem of the difficult control of Fusarium head blight of wheat, but also delay the pathogen from developing resistance to it. The inventor combined validamycin and epoxiconazole and found that it has excellent antibacterial activity against Fusarium head blight of wheat within a suitable range, can significantly improve the control effect, reduce the number of pesticide applications and the dosage of pesticides, slow down the development of pathogen resistance, and reduce the control cost. Summary of the Invention

[0006] To solve the problems existing in the prior art, the inventor compounded validamycin and epoxiconazole in a certain mass ratio, which can produce a synergistic effect, effectively reducing the amount of pesticide used and the control cost, and at the same time greatly reducing the pesticide residue in agricultural products. Based on the above research, the applicant further completed the present invention.

[0007] The present invention combines two types of bactericides with different modes of action and processes them into a formulation that is easy to use in production. Compared with the separate use of the two components, the bactericidal composition has high biological activity, the total dosage of pesticides per unit area decreases, the application cost is greatly reduced, and at the same time, the emergence and development of pathogen resistance are delayed to a greater extent, the risk of the pathogenic fungus developing resistance to a single bactericide is reduced, and the service life of each component in the bactericidal composition is extended.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] In the first aspect of the present invention, a bactericidal composition is provided, and the active ingredients are composed of validamycin and epoxiconazole; on the basis of the above scheme, the mass ratio of validamycin to epoxiconazole is 1:9 to 9:1; preferably, the mass ratio of validamycin to epoxiconazole is 1:1 to 1:7, and more preferably, the mass ratio of validamycin to epoxiconazole is 1:1.

[0010] In the second aspect of the present invention, a formulation containing the bactericidal composition of validamycin and epoxiconazole is provided. In addition to the active ingredients, the bactericidal composition contains adjuvants, and the mass percentage content of the active ingredients in the bactericidal composition is 20% to 32%.

[0011] The bactericidal composition containing validamycin and epoxiconazole is characterized in that: the dosage form of the bactericidal composition is emulsifiable concentrate and suspension concentrate.

[0012] In the third aspect of the present invention, an application of the above formulation in the control of Fusarium head blight of wheat is provided.

[0013] Adopting the above technical solution, the present invention has the following beneficial effects:

[0014] (1) The present invention has a higher control effect on wheat scab, and there is an obvious synergistic effect between the bactericidal composition containing xinjubutramide and epoxiconazole compared with the single agent; (2) The amount of pesticide used is reduced, effectively reducing ecological damage and environmental pollution, and can improve the yield and quality of crops; (3) It can effectively control the resistance of pathogenic bacteria such as Fusarium to fungicides such as carbendazim and prochloraz, and can be rotated with a variety of existing agents, delaying the development and spread of resistance of pathogenic bacteria to existing agents and single agents, which is of great significance for the comprehensive control of various diseases. Specific embodiments

[0015] The following further publicly describes the specific solutions and effects of the present invention in combination with specific embodiments.

[0016] Example 1: Indoor bioassay of xinjubutramide and epoxiconazole against wheat scab pathogen

[0017] Test strain: The wheat scab pathogen (Fusarium graminearum) was provided by the Bactericide Biology Laboratory of Anhui Agricultural University.

[0018] Agent preparation: The test agents were dissolved in acetone to prepare single-agent stock solutions and mixed-agent stock solutions of each ratio. Five mass concentration gradients were set for each single agent and mixed agent of each ratio by the equal ratio method.

[0019] Test method: Referring to the pesticide indoor bioassay test guidelines NY / T 1156.2-2006, the petri dish method was used. The pure cultured wheat scab pathogen was cut into agar plugs with a diameter of 5 mm under sterile conditions using a sterilized puncher at the edge of the colony. The agar plugs were inoculated in the center of the drug-containing petri dish with an inoculator, with the mycelium side facing down, and each treatment had 4 replicates. It was cultured in a constant temperature incubator at 25±1°C. When the diameter of the blank control colony was about 5 cm, the colony diameter was measured with a digital vernier caliper. The diameter of each colony was measured vertically once by the cross method, and the average value was taken.

[0020] Data analysis: According to the investigation results, the growth inhibition rate of each treatment was calculated according to the formula. The calculation formula is as follows:

[0021] Growth inhibition rate (%) = (control colony diameter - treatment colony diameter) / (control colony diameter - agar plug diameter) × 100

[0022] According to the logarithm of the agent concentration and the probit value of the corresponding growth inhibition rate of the pathogenic bacteria, regression analysis was performed using the DPS data processing system to calculate the EC 50 value.

[0023] According to the EC of the two single agents 50Set the combination ratio of the two agents according to the Wadley method, calculate the synergistic coefficient (SR) according to the Wadley method, and make a comprehensive evaluation based on this. SR ≤ 0.5 indicates an antagonistic effect, 0.5 < SR < 1.5 indicates an additive effect, and SR ≥ 1.5 indicates a synergistic effect. The calculation method of the SR value is as follows:

[0024] EC 50 (Theoretical value) = (a + b) / (a / EC 50A + b / EC 50B );

[0025] SR = EC 50 (Theoretical value) / EC 50 (Actual value);

[0026] SR is the synergistic coefficient, and a and b are the percentage contents of each component in the mixture.

[0027] Results and analysis:

[0028] As can be seen from Table 1, when the mass ratio of cymoxanil to epoxiconazole is 1:1, 1:3, 1:5, and 1:7, the synergistic coefficients (SR) are 2.3187, 1.5257, 1.5095, and 1.8982 respectively. This indicates that the cymoxanil and epoxiconazole bactericidal composition shows a synergistic effect against Gibberella zeae within the range of the mass ratio from 1:1 to 1:7. Among them, the maximum synergistic ratio is 1:1, the synergistic coefficient is the largest, and the synergistic effect is the most obvious.

[0029] Table 1 Indoor bioassay results of the compound of cymoxanil and epoxiconazole against Gibberella zeae

[0030]

[0031] Example two: Formulation example

[0032] Example 1: Preparation of 20% cymoxanil·epoxiconazole emulsifiable concentrate

[0033] By mass percentage, the proportion of each component is: 10% cymoxanil, 10% epoxiconazole, 27% cyclohexane (solvent), 4% calcium dodecylbenzenesulfonate (emulsifier), 7% castor oil polyethylene glycol condensate (emulsifier), 5% glycerol (antifreeze), and xylene (filler) to make up the balance.

[0034] Preparation method: Prepare materials according to the above ratio and add them to a mixing kettle by the conventional emulsifiable concentrate processing preparation method, and stir evenly to make 20% cymoxanil·epoxiconazole emulsifiable concentrate.

[0035] Example 2: Preparation of 20% cymoxanil·epoxiconazole emulsifiable concentrate

[0036] By mass percentage, the component ratios are as follows: 5% of cymoxanil, 15% of epoxiconazole, 27% of cyclohexane (solvent), 4% of calcium dodecylbenzenesulfonate (emulsifier), 7% of castor oil polyethylene glycol condensate (emulsifier), 5% of glycerol (antifreeze), and the balance is made up with xylene (filler).

[0037] Preparation method: Prepare materials according to the above ratios and prepare 20% cymoxanil·epoxiconazole EC by the conventional EC processing preparation method.

[0038] Example 3: Preparation of 24% cymoxanil·epoxiconazole SC

[0039] By mass percentage, the component ratios are as follows: 4% of cymoxanil, 20% of epoxiconazole, 3% of alkylnaphthalenesulfonate (dispersant), 3% of fumed silica (thickener), 2% of butyl epoxy stearate (stabilizer), 1% of silicone (antifoaming agent), 1% of propylene glycol (antifreeze), and the balance is made up with water.

[0040] Preparation method: Prepare materials according to the above ratios and prepare 24% cymoxanil·epoxiconazole SC by the conventional SC processing preparation method.

[0041] Example 4: Preparation of 32% cymoxanil·epoxiconazole SC

[0042] By mass percentage, the component ratios are as follows: 4% of cymoxanil, 28% of epoxiconazole, 3% of alkylnaphthalenesulfonate (dispersant), 3% of fumed silica (thickener), 2% of butyl epoxy stearate (stabilizer), 1% of silicone (antifoaming agent), 1% of propylene glycol (antifreeze), and the balance is made up with water.

[0043] Preparation method: Prepare materials according to the above ratios and prepare 32% cymoxanil acetate·epoxiconazole SC by the conventional SC processing preparation method.

[0044] Example 3: Field control efficacy

[0045] In order to further verify the actual application effect of the present invention, the bactericidal compositions containing cymoxanil and epoxiconazole prepared in Examples 1-4 were used respectively, with cymoxanil and epoxiconazole as control agents, and a field efficacy test for controlling wheat scab was carried out.

[0046] Test treatment method: The field control efficacy test for wheat scab was carried out in the suburbs of Bengbu City, with a test area of 0.9 hm 2 , and the medicament was sprayed during the wheat flowering stage, with spraying clear water as the control. The medicament was applied 2 times at an interval of 7 days, and each treatment was repeated three times.

[0047] Efficacy investigation and calculation method: The disease incidence was investigated after the occurrence of scab was stable. Diagonal five-point sampling was used, and 100 ears were investigated for each treatment.

[0048] The grading standard for the severity of diseased ears is as follows:

[0049] Level 1 diseased spikelets account for less than 25% of the whole spike.

[0050] Level 2 diseased spikelets account for 25% to 50% of the entire spike.

[0051] Grade 3 diseased spikelets account for 50% to 75% of the entire spike.

[0052] Level 4 diseased spikelets account for more than 75% of the whole spike. The disease index and control effect calculation formula are:

[0053] Disease index = [Σ(number of diseased ears at each level × corresponding disease level value) / total number of ears surveyed × highest level representative value] × 100;

[0054] Control effect = [(control disease index - treatment disease index) / control disease index] × 100%.

[0055] Results and Analysis:

[0056] As can be seen from Table 2, the fungicidal composition of oxazolidinone and fluconazole showed excellent control effect on wheat fusarium head blight. Under the same dosage conditions of oxazolidinone, the control agents oxazolidinone (40.13%) and fluconazole (76.42%) had lower control effects, while the control effects of Examples 1-4 were all over 85%. The control effects of each treatment of the fungicidal composition were significantly higher than those of the control agent, showing a synergistic effect.

[0057] Table 2 Field efficacy of the fungicidal combination of cypermethrin and epoxiconazole in controlling wheat scab

[0058]

[0059] The results of the indoor activity test and the field test above show that the fungicidal composition containing oxadiazine and epoxiconazole described in the present invention shows good control effect on wheat fusarium head blight, and the obtained fungicidal composition or preparation shows obvious synergistic effect. The fungicidal composition obtained by compounding the present invention can delay the resistance of wheat fusarium head blight to a single agent, reduce the dosage of the drug, effectively reduce ecological damage and environmental pollution, and improve crop yield and quality.

[0060] Finally, it should be noted that the above examples are only some specific embodiments of the present invention. Obviously, the present invention is not limited to the above embodiments, and there are many variations. All variations that can be directly derived or associated with the content disclosed by a person skilled in the art are considered to be within the scope of protection of the present invention.

Claims

1. A bactericidal composition containing oxazolidinone and fluopicolide, characterized in that: The active ingredients are composed of sinotriptyline and fluopyram, and the mass ratio of sinotriptyline to fluopyram is 1:1 to 1:

7.

2. The bactericidal composition according to claim 1, characterized in that: The mass ratios of oxathiapiprolin and epoxiconazole in the bactericidal composition are 1:1, 1:3, 1:5 and 1:

7.

3. The bactericidal composition according to claim 1, characterized in that: The mass ratio of oxathiapiprolin to epoxiconazole in the bactericidal composition is 1:

1.

4. The bactericidal composition according to claim 1, characterized in that: Also contains agricultural auxiliary ingredients.

5. The bactericidal composition according to claim 4, characterized in that: The dosage form of the bactericidal composition is emulsifiable concentrate, suspension, microemulsion, aqueous emulsion, water-dispersible granules and wettable powder.

6. Use of the fungicidal composition according to any one of claims 1 to 5 for preventing and controlling crop diseases.

7. The use according to claim 6, characterized in that The crop disease is wheat fusarium rust.

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