Quantitative blood coagulation vacuum blood collection tube and use method thereof

Abstract

The invention relates to the technical field of vacuum blood collection tubes, and discloses a quantitative blood clotting vacuum blood collection tube and a using method thereof.The quantitative blood clotting vacuum blood collection tube comprises a blood collection tube body and a rubber plug arranged on the blood collection tube body, a liquid storage gap is formed in the side wall of the blood collection tube body, and a communicating opening communicating with the liquid storage gap is further formed in the inner wall of the blood collection tube body; a one-way valve is arranged in the communicating port, and the conducting direction of the one-way valve is one-way conducting from the inside of the blood collection tube body to the liquid storage gap; the position of the communicating port meets the requirement that redundant blood flows into the communicating port when the blood volume in the blood collection tube body exceeds a fixed value; by arranging the liquid storage gap, the communicating port and the one-way valve and specially arranging the position of the communicating port, a blood sampling person can know that the blood collection amount reaches a fixed value when observing that blood flows into the liquid storage gap, and the blood entering the liquid storage gap is limited by the one-way valve and cannot flow back; the method has the advantages of realizing quantitative detection of blood volume and ensuring the accuracy of subsequent detection results.

Description

Technical Field

[0001] The present invention relates to a quantitative blood coagulation vacuum blood collection tube and a method for using the same, belonging to the technical field of vacuum blood collection tubes. Background Art

[0002] A blood coagulation vacuum blood collection tube is a blood collection tube specifically used for blood coagulation function detection, which usually contains specific anticoagulants and additives to ensure the stability of blood samples during collection, transportation, and storage, and enable accurate laboratory tests.

[0003] Currently, blood coagulation vacuum blood collection tubes are mainly used for common coagulation item detections (such as prothrombin time, thrombin time, activated partial thromboplastin time, fibrinogen, D-dimer, etc.), guiding clinical monitoring (such as monitoring of oral warfarin), monitoring of intravenous heparin infusion, and special coagulation research (such as detection of coagulation factors: factor VIII or hemophilia factor, platelet function detection, etc.). The blood collection principle of such blood coagulation vacuum blood collection tubes is as follows: by evacuating the inside of the blood coagulation vacuum blood collection tube to a vacuum state, when it is connected to a blood collection needle, blood will be pressed into the blood coagulation vacuum blood collection tube for collection under the action of a pressure difference. Currently, a common problem with such blood coagulation vacuum blood collection tubes is that it is difficult to complete quantitative blood collection.

[0004] For this reason, a patent application for an invention with the Chinese patent publication number CN117503132A discloses a vacuum blood collection tube for quantitative blood collection. By setting a blood volume plate and a balance mechanism, the setting of the blood volume plate can more intuitively show whether the blood collection volume meets the standard. When the negative pressure in the vacuum blood collection tube is insufficient and the blood collection volume is insufficient, it controls the movement of the piston through a bolt, thereby being able to supplement the negative pressure and perform precise blood collection.

[0005] However, it still has the following problems in use: Since there is a limited amount of blood required for coagulation item detections, taking the common citrate anticoagulant (0.2 ml, 109 mmol / L) as an example, calculated according to the ratio of citrate anticoagulant (0.2 ml, 109 mmol / L) to blood of 1:9, the required final blood volume is 1.8 ml. If the collected blood volume exceeds the limit, it will affect the test results. For the technical solution provided by the above-mentioned patent application for an invention, when adjusting the internal pressure through the piston, it is very easy to have a situation where too much blood is drawn, which will cause the problem that the blood collection volume exceeds the limit during clinical blood collection, affecting subsequent test results. Summary of the Invention

[0006] In order to solve the above problems existing in the prior art, the present invention provides a quantitative blood coagulation vacuum blood collection tube and a method for using the same.

[0007] The technical solution of the present invention is as follows: In a first aspect, the present invention provides a quantitative blood coagulation vacuum blood collection tube, which includes a blood collection tube body and a rubber stopper disposed at the mouth position of the blood collection tube body. A liquid storage gap is formed in the side wall of the blood collection tube body, and a communication port communicating with the liquid storage gap is further formed on the inner wall of the blood collection tube body. A one-way valve is disposed inside the communication port, and the conduction direction of the one-way valve is a one-way conduction from inside the blood collection tube body to the liquid storage gap; the position of the communication port is set such that when the blood volume inside the blood collection tube body exceeds a certain value, the excess blood will flow into the inside of the communication port.

[0008] Further, a plug-in groove adapted to the structure of the mouth of the blood collection tube body is formed at the bottom of the rubber stopper, and the mouth of the blood collection tube body is inserted into the plug-in groove.

[0009] Further, a positioning groove is further formed at the top of the rubber stopper, and a guiding sleeve is embedded at the bottom of the rubber stopper. The guiding sleeve is of a conical structure, the small end of the guiding sleeve is close to the blood collection tube body, and the guiding sleeve is coaxially arranged with the positioning groove.

[0010] Further, the blood collection tube body is of an integrally formed structure.

[0011] Further, the blood collection tube body is of a split structure. The blood collection tube body includes a first tube body and a second tube body. The first tube body is disposed above the second tube body, and the bottom of the first tube body and the top of the second tube body are hermetically connected together by a sealing connection assembly.

[0012] Further, the liquid storage gap is formed in the side wall of the second tube body, and the liquid storage gap is opened inward from the top of the second tube body. The liquid storage gap is arranged in a circular ring shape in cross section in the second tube body. The communication port is formed at the bottom side wall position of the first tube body, and the communication port also communicates the side wall of the first tube body with the bottom wall of the first tube body. When the first tube body and the second tube body are hermetically connected together by the sealing connection assembly, the bottom of the communication port is communicated with the bottom of the liquid storage gap.

[0013] Further, the sealing connection assembly includes a first connection sleeve fixedly sleeved at the bottom of the first tube body and a fixed sleeve fixedly sleeved at the top of the second tube body. A connection groove with a circular ring-shaped cross section is formed inward on the bottom wall of the first connection sleeve, and a first connection thread is formed on the outer side wall of the connection groove. A second connection sleeve is fixedly arranged at the top of the fixed sleeve. The outer diameter of the second connection sleeve is adapted to the outer diameter of the connection groove, and a second connection thread adapted to the first connection thread is formed on the outer side wall of the second connection sleeve. The second connection sleeve can be connected to the connection groove by a threaded connection between the second connection thread and the first connection thread.

[0014] Furthermore, a sealing gap is left between the inner side wall of the second connecting sleeve and the inner side wall of the connecting groove. A sealing rubber ring is fixedly arranged in the sealing gap. When the second connecting sleeve is connected into the connecting groove, the outer side wall of the sealing rubber ring can be tightly attached to the inner side wall of the second connecting sleeve, and the bottom wall of the sealing rubber ring can be tightly attached to the top wall of the fixed sleeve.

[0015] Furthermore, the bottom of the sealing rubber ring extends out of the connecting groove. The outer diameter of the sealing rubber ring is larger than the inner diameter of the second connecting sleeve. A guiding inclined wall is also arranged on the outer side wall of the bottom of the sealing rubber ring. The arrangement of the guiding inclined wall makes the outer diameter at the bottom position of the sealing rubber ring smaller than the inner diameter of the second connecting sleeve.

[0016] In a second aspect, the present invention provides a method for using the aforementioned quantitative blood coagulation vacuum blood collection tube, including the following steps: The first step: Determine the pressure value inside the blood collection tube body; the pressure value inside the blood collection tube body is calculated according to the following formula:

[0017] In the formula, represents the pressure value inside the blood collection tube body, represents the viscosity value of the blood, taking 4 mPa·s, L represents the length of the blood collection tube body, Q represents the volume of blood to be collected, and r represents the radius of the blood collection tube body.

[0018] The second step: Extract the pressure inside the blood collection tube body to the pressure value calculated in the first step; The third step: Insert an external blood collection needle through the rubber stopper into the blood collection tube body, and then blood collection can be carried out. When it is observed that blood flows into the liquid storage gap through the one-way valve, stop blood collection by pulling out the external blood collection needle. The present invention has the following beneficial effects: 1. By arranging a liquid storage gap in the side wall of the blood collection tube body, arranging a communication port communicating with the liquid storage gap on the inner wall of the blood collection tube body, arranging a one-way valve in the communication port, and arranging the position of the communication port in a special way, when the blood volume in the blood collection tube body exceeds a certain value, the excess blood will flow into the communication port and then into the liquid storage gap through the one-way valve for collection. When the blood collection personnel observe that blood flows into the liquid storage gap, they can know that the blood collection volume has reached the fixed value, and then stop blood collection. The blood flowing into the liquid storage gap is restricted by the one-way conduction of the one-way valve and cannot flow back into the blood collection tube body, so as to ensure the constancy of the blood volume in the blood collection tube body and not exceed the limited amount specified in the experiment. Compared with the prior art, it has the advantages of realizing quantitative detection of blood volume and ensuring the accuracy of subsequent detection results.

[0019] 2. The present invention provides a method for using a quantitative blood coagulation vacuum blood collection tube, which calculates the pressure value in the blood collection tube body by setting a specific formula to ensure that the negative pressure in the blood collection tube body can draw enough blood during subsequent blood drawing. In combination with the use of the blood collection tube body, it can avoid the occurrence of too little or too much blood drawing, so as to achieve quantitative detection of blood volume and ensure the accuracy of subsequent test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the structure of Embodiment 1 of the present invention; Figure 2 This is a schematic diagram of the structure of Embodiment 2 of the present invention; Figure 3 This is a schematic structural diagram of the first tube body and the second tube body being connected in the second embodiment of the present invention; Figure 4 This is the enlarged view of point A; Figure 5 This is an enlarged view of point B.

[0021] The reference numerals in the figure represent: 1. Blood collection tube body; 2. Rubber plug; 3. Liquid storage gap; 4. Connecting port; 5. One-way valve; 6. Plug-in slot; 7. Positioning slot; 8. Guide sleeve; 9. First tube body; 10. Second tube body; 11. First connecting sleeve; 12. Fixed sleeve; 13. Connecting slot; 14. First connecting thread; 15. Second connecting sleeve; 16. Second connecting thread; 17. Sealing gap; 18. Sealing rubber ring; 19. Guide inclined wall. DETAILED DESCRIPTION

[0022] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0023] Example 1: Please refer to Figure 1 The present embodiment provides a quantitative blood coagulation vacuum blood collection tube, comprising a blood collection tube body 1 and a rubber stopper 2 arranged at the tube mouth of the blood collection tube body 1. The bottom of the rubber stopper 2 is provided with an insertion groove 6 which is adapted to the tube mouth structure of the blood collection tube body 1. The tube mouth of the blood collection tube body 1 is inserted into the insertion groove 6. The rubber stopper 2 is connected to the tube mouth of the blood collection tube body 1 through the insertion groove 6 so as to be fixedly connected to the blood collection tube body 1. The rubber stopper 2 can seal the blood collection tube body 1.

[0024] In this embodiment, the blood collection tube body 1 is of an integrally formed structure. A liquid storage gap 3 is formed inside the side wall of the blood collection tube body 1. A communication port 4 communicating with the liquid storage gap 3 is further formed on the inner wall of the blood collection tube body 1. A one-way valve 5 is arranged inside the communication port 4, and the conduction direction of the one-way valve 5 is one-way conduction from inside the blood collection tube body 1 to the liquid storage gap 3. The position of the communication port 4 is set such that when the blood volume inside the blood collection tube body 1 exceeds a certain value, the excess blood will flow into the communication port 4. At the same time, in order to enable the blood entering the communication port 4 to smoothly flow into the liquid storage gap 3, in this embodiment, the position where the communication port 4 communicates with the liquid storage gap 3 is at the top of the liquid storage gap 3, so that the blood entering the communication port 4 can smoothly flow into the liquid storage gap 3 under the action of gravity.

[0025] To facilitate the subsequent blood collection work, a positioning groove 7 is further formed at the top of the rubber stopper 2. The positioning groove 7 is used for positioning when a blood collection needle is inserted subsequently. The positioning groove 7 is arranged in an arc structure, and the central axis of the positioning groove 7 is coaxially arranged with the central axis of the blood collection tube body 1. An induction sleeve 8 is further embedded at the bottom of the rubber stopper 2. The induction sleeve 8 is arranged in a conical structure. The small end of the induction sleeve 8 is arranged close to the blood collection tube body 1. The central axis of the induction sleeve 8 is also coaxially arranged with the central axis of the positioning groove 7. The arrangement of the induction sleeve 8 is used to guide the blood collection needle to pass through the rubber stopper 2, avoiding phenomena such as deviation when the blood collection needle passes through the rubber stopper 2, which may affect the normal progress of the blood collection work.

[0026] This embodiment also provides a method for using a quantitative blood coagulation vacuum blood collection tube, including the following steps: The first step: Determine the pressure value inside the blood collection tube body 1. The pressure value inside the blood collection tube body 1 is calculated according to the following formula:

[0027] In the formula, represents the pressure value inside the blood collection tube body 1, represents the viscosity value of the blood, taking 4 mPa·s. L represents the length of the blood collection tube body 1. Q represents the volume of the blood volume to be collected. r represents the radius of the blood collection tube body 1. For example, taking the common citric acid anticoagulant of 0.2 ml and 109 mmol / L as an example, according to the ratio of citric acid anticoagulant of 0.2 ml and 109 mmol / L to blood of 1:9, the required final blood volume is 1.8 ml. The value of 1.8 ml can be used as the value of Q, combined with the actual length L and actual radius r of the blood collection tube body 1, and then can be calculated. At the same time, to ensure the sufficiency of the drawn blood volume and to overcome the situation of pressure value change of the blood collection tube body 1 caused by possible subsequent operation errors, in actual calculation, the blood volume can be set to 1.9 - 2.0 ml for substitution calculation.

[0028] Step 2: Use a common vacuum device to extract the pressure inside the blood collection tube body 1 to the pressure value calculated in Step 1.

[0029] Step 3: During blood collection, the blood collection personnel place the blood collection tube body 1 on the placement rack, and then insert the end of the external blood collection needle inserted into the patient's blood vessel through the rubber stopper 2 into the blood collection tube body 1. At this time, the negative pressure existing in the blood collection tube body 1 can extract the blood in the patient's blood vessel through the blood collection needle to perform corresponding blood collection work. The blood collection personnel should observe the blood volume in the blood collection tube body 1 during the blood collection process. When it is observed that blood flows into the liquid storage gap 3 through the one-way valve 5, stop blood collection by pulling out the external blood collection needle. At this time, the blood volume in the blood collection tube body 1 is the required fixed value. The blood entering the liquid storage gap 3 is restricted by the one-way conduction of the one-way valve 5 and cannot flow back into the blood collection tube body 1, thus ensuring the constancy of the blood volume in the blood collection tube body 1 and not exceeding the limited quantity specified in the experiment, having the advantages of realizing quantitative detection of blood volume and ensuring the accuracy of subsequent detection results.

[0030] Since it is relatively difficult to process the liquid storage gap 3 in the integrally formed blood collection tube body 1, it mostly needs to be made by chemical etching method or laser processing method. The production cost required by the chemical etching method or laser processing method is relatively high, and the rejection rate is also relatively high. Therefore, on the basis of Embodiment 1, Embodiment 2 is proposed.

[0031] Embodiment 2: Please refer to Figures 2 to 5 , this embodiment provides a quantitative blood coagulation vacuum blood collection tube, which is different from Embodiment 1 in that: the blood collection tube body 1 is of a split structure.

[0032] In this embodiment, the blood collection tube body 1 includes a first tube body 9 and a second tube body 10. The first tube body 9 is arranged above the second tube body 10. The rubber stopper 2 is connected to the pipe orifice position of the first tube body 9. The liquid storage gap 3 is opened in the side wall of the second tube body 10, and the liquid storage gap 3 is opened inward from the top of the second tube body 10. The liquid storage gap 3 is arranged in a circular cross-section in the second tube body 10. The communication port 4 is opened at the bottom side wall position of the first tube body 9, and the communication port 4 also communicates the side wall of the first tube body 9 with the bottom wall of the first tube body 9. The bottom of the first tube body 9 and the top of the second tube body 10 are hermetically connected together by setting a sealing connection assembly. When the first tube body 9 and the second tube body 10 are hermetically connected together by the sealing connection assembly, the bottom of the communication port 4 is communicated with the bottom of the liquid storage gap 3 so that the blood entering the communication port 4 can smoothly flow into the liquid storage gap 3.

[0033] Through the foregoing settings, by setting the first tube body 9 and the second tube body 10 in a split structure, when manufacturing this vacuum blood collection tube, the first tube body 9 and the second tube body 10 can be directly processed by a hot processing method through a corresponding mold. Since the liquid storage gap 3 is opened inward from the top of the second tube body 10, there is no need to perform an operation of creating an internal channel, that is, there is no need to use chemical etching or laser processing methods. The hot processing method has the advantages of low cost and high cost rate, so as to reduce the manufacturing cost of this vacuum blood collection tube.

[0034] In this embodiment, the sealed connection assembly includes a first connection sleeve 11 fixedly sleeved on the bottom of the first tube body 9 and a fixed sleeve 12 fixedly sleeved on the top of the second tube body 10. A connection groove 13 with an annular cross-section is opened inward on the bottom wall of the first connection sleeve 11. A first connection thread 14 is opened on the outer side wall of the connection groove 13. A second connection sleeve 15 is fixedly provided on the top of the fixed sleeve 12. The outer diameter of the second connection sleeve 15 is adapted to the outer diameter of the connection groove 13. A second connection thread 16 adapted to the first connection thread 14 is opened on the outer side wall of the second connection sleeve 15. The second connection sleeve 15 can be connected to the connection groove 13 through the threaded connection of the second connection thread 16 and the first connection thread 14. When the second connection sleeve 15 is completely connected into the connection groove 13, at this time, the bottom of the first tube body 9 can be tightly attached to the top of the second tube body 10. At this time, the first tube body 9 and the second tube body 10 can be connected together.

[0035] A sealing gap 17 is left between the inner side wall of the second connection sleeve 15 and the inner side wall of the connection groove 13. A sealing rubber ring 18 is fixedly provided in the sealing gap 17. The bottom of the sealing rubber ring 18 extends out of the connection groove 13. The outer diameter of the sealing rubber ring 18 is larger than the inner diameter of the second connection sleeve. When the second connection sleeve 15 is connected into the connection groove 13, the sealing rubber ring 18 can generate a corresponding deformation after being squeezed by the second connection sleeve 15 and the fixed sleeve 12. At this time, the outer side wall of the sealing rubber ring 18 can be tightly attached to the inner side wall of the second connection sleeve 15, and the bottom wall of the sealing rubber ring 18 can be tightly attached to the top wall of the fixed sleeve 12, so as to achieve the required sealing effect.

[0036] To facilitate the connection of the second connection sleeve 15 into the connection groove 13, a guiding inclined wall 19 is further provided on the outer side wall of the bottom of the sealing rubber ring 18. The setting of the guiding inclined wall 19 makes the outer diameter of the bottom position of the sealing rubber ring 18 smaller than the inner diameter of the second connection sleeve. The setting of the guiding inclined wall 19 can play a guiding role for the second connection sleeve 15, making it more convenient to align with the connection groove 13.

[0037] Through the setting of the foregoing sealing connection assembly, when connecting the first pipe body 9 and the second pipe body 10, by aligning the second connection sleeve 15 with the connection groove 13, then inserting the second connection sleeve 15 into the connection groove 13 and rotating it, so that the second connection sleeve 15 gradually enters the connection groove 13 under the action of the first connection thread 14 and the second connection thread 16, and stops rotating until it cannot rotate anymore. At this time, the bottom of the first pipe body 9 is tightly attached to the top of the second pipe body 10, the outer side wall of the rubber sealing ring is tightly attached to the inner side wall of the second connection sleeve 15, and the bottom wall of the sealing rubber ring 18 is tightly attached to the top wall of the fixed sleeve 12, so as to be able to connect the first pipe body 9 and the second pipe body 10 and block the gap between the first pipe body 9 and the second pipe body 10, thereby realizing the sealing connection between the first pipe body 9 and the second pipe body 10.

[0038] The above are only the embodiments of the present invention, and do not limit the patent scope of the present invention accordingly. Any equivalent structure or equivalent process transformation made by using the content of the specification and drawings of the present invention, or directly or indirectly applied to other related technical fields, shall be equally included in the patent protection scope of the present invention.

Claims

1. A quantitative blood coagulation vacuum blood collection tube, comprising a blood collection tube body (1) and a rubber stopper (2) arranged at the tube opening of the blood collection tube body (1), characterized in that: A liquid storage gap (3) is provided in the side wall of the blood sampling tube body (1), and a communication port (4) is provided on the inner wall of the blood sampling tube body (1) and is connected to the liquid storage gap (3). A one-way valve (5) is provided inside the communication port (4), and the conduction direction of the one-way valve (5) is one-way conduction from the blood sampling tube body (1) to the liquid storage gap (3); the position of the communication port (4) is set to ensure that when the amount of blood in the blood sampling tube body (1) exceeds a fixed value, excess blood will flow into the inside of the communication port (4).

2. A quantitative blood coagulation vacuum blood collection tube according to claim 1, characterized in that: The bottom of the rubber stopper (2) is provided with an inserting groove (6) adapted to the tube mouth structure of the blood collection tube body (1), and the tube mouth of the blood collection tube body (1) is inserted into the inserting groove (6).

3. A quantitative blood coagulation vacuum blood collection tube according to claim 1, characterized in that: The top of the rubber stopper (2) is also provided with a positioning groove (7), and the bottom of the rubber stopper (2) is also provided with a guide sleeve (8) in an embedded manner. The guide sleeve (8) is provided in a conical structure, and the small end of the guide sleeve (8) is provided close to the blood collection tube body (1). The guide sleeve (8) is also provided coaxially with the positioning groove (7).

4. A quantitative blood coagulation vacuum blood collection tube according to claim 1, characterized in that: The blood collection tube body (1) is an integrally formed structure.

5. The quantitative blood coagulation vacuum blood collection tube according to claim 1, characterized in that: The blood collection tube body (1) is of a split structure, comprising a first tube body (9) and a second tube body (10), wherein the first tube body (9) is arranged above the second tube body (10), and the bottom of the first tube body (9) and the top of the second tube body (10) are sealed together by providing a sealing connection assembly.

6. A quantitative blood coagulation vacuum blood collection tube according to claim 5, characterized in that: The liquid storage gap (3) is formed in the side wall of the second tube body (10), and the liquid storage gap (3) is formed inwardly from the top of the second tube body (10). The liquid storage gap (3) is arranged in the second tube body (10) in a circular ring shape in cross section. The communication port (4) is formed in the bottom side wall of the first tube body (9), and the communication port (4) also connects the side wall of the first tube body (9) with the bottom wall of the first tube body (9). When the first tube body (9) and the second tube body (10) are sealed together by means of a sealing connection assembly, the bottom of the communication port (4) is connected to the bottom of the liquid storage gap (3).

7. The quantitative blood coagulation vacuum blood collection tube according to claim 5, characterized in that: The sealing connection assembly comprises a first connection sleeve (11) fixedly sleeved on the bottom of the first tube body (9) and a fixed sleeve (12) fixedly sleeved on the top of the second tube body (10); a connection groove (13) with a circular cross-section is provided inwardly on the bottom wall of the first connection sleeve (11); a first connection thread (14) is provided on the outer side wall of the connection groove (13); a second connection sleeve (15) is fixedly provided on the top of the fixed sleeve (12); the outer diameter of the second connection sleeve (15) is adapted to the outer diameter of the connection groove (13); a second connection thread (16) adapted to the first connection thread (14) is provided on the outer side wall of the second connection sleeve (15); the second connection sleeve (15) can be connected to the connection groove (13) through the threaded connection between the second connection thread (16) and the first connection thread (14).

8. The quantitative blood coagulation vacuum blood collection tube according to claim 7, characterized in that: A sealing gap (17) is left between the inner side wall of the second connecting sleeve (15) and the inner side wall of the connecting groove (13), and a sealing rubber ring (18) is fixedly arranged in the sealing gap (17). When the second connecting sleeve (15) is connected to the connecting groove (13), the outer side wall of the sealing rubber ring (18) can be closely arranged with the inner side wall of the second connecting sleeve (15), and the bottom wall of the sealing rubber ring (18) can be closely arranged with the top wall of the fixed sleeve (12).

9. A quantitative blood coagulation vacuum blood collection tube according to claim 8, characterized in that: The bottom of the sealing rubber ring (18) is arranged to extend out of the connecting groove (13), the outer diameter of the sealing rubber ring (18) is arranged to be larger than the inner diameter of the second connecting sleeve, and a guiding inclined wall (19) is also arranged on the outer side wall of the bottom of the sealing rubber ring (18). The arrangement of the guiding inclined wall (19) makes the outer diameter of the bottom position of the sealing rubber ring (18) smaller than the inner diameter of the second connecting sleeve.

10. A method for using the quantitative blood coagulation vacuum blood collection tube according to any one of claims 1 to 9, comprising the following steps: Step A1: Determine the internal pressure value of the blood collection tube body (1); the internal pressure value of the blood collection tube body (1) is calculated according to the following formula: In the formula, Indicates the pressure value inside the blood collection tube body (1). represents the viscosity of blood, which is 4 mPa·s, L represents the length of the blood collection tube body (1), Q represents the volume of blood to be collected, and r represents the radius of the blood collection tube body (1). Step A2: reducing the pressure in the blood collection tube body (1) to the pressure value calculated in step A1; Step A3: insert the external blood collection needle through the rubber stopper (2) into the blood collection tube body (1) to start blood collection. When blood is observed to flow into the liquid storage gap (3) through the one-way valve (5), the external blood collection needle is pulled out to stop blood collection.

Images