Bionic hydrogel dressing and preparation method thereof

Abstract

The invention relates to the technical field of hydrogel, in particular to bionic hydrogel dressing and a preparation method thereof.The bionic hydrogel dressing is prepared from a hydrogel matrix, polylysine, phenylalanine-based gel, propylene glycol and deionized water, the invention discloses an antibacterial hydrogel dressing, which is prepared from the following components in parts by weight: 1 to 5 parts of hydrogel matrix, 0.1 to 1 part of polylysine, 0.01 to 0.1 part of phenylalanine-based gel, 1 to 5 parts of propylene glycol and 89 to 98 parts of deionized water. The antibacterial hydrogel dressing can effectively solve the problems that the existing hydrogel dressing still lacks structural bionics in the use process, is difficult to promote cell regeneration and wound surface tissue healing, has no antibacterial property, is difficult to promote wound surface tissue healing, and cannot be used for treating the wound surface tissue healing. And once bacterial infection occurs, a stable environment for cell regeneration cannot be maintained, and enough mechanical stability and self-repairing capability are lacked.

Description

Technical Field

[0001] The present invention relates to the technical field of hydrogels, and specifically relates to a bionic hydrogel dressing and a preparation method thereof. Background Art

[0002] Hydrogel wound dressings have a three-dimensional network structure, can simulate the extracellular matrix to form an environment for maintaining cell physiological activities, have good elasticity and hydrophilicity, and have the characteristics of being soft and highly plastic. Compared with traditional dressings that only play a simple physical isolation role, hydrogel dressings can not only cover the wound and provide a temporary barrier to resist external infections, but also effectively control fluid loss and provide a moist environment during the wound repair process.

[0003] However, existing hydrogel dressings still have deficiencies, specifically: existing hydrogel dressings still lack structural bionics during use, are difficult to promote cell regeneration and wound tissue healing, have no antibacterial properties, and once infected with bacteria, they cannot maintain a stable environment for cell regeneration, and lack sufficient mechanical stability and self-healing ability; Therefore, a bionic hydrogel dressing and a preparation method thereof are needed to solve the problems raised in the above background art. Summary of the Invention

[0004] The purpose of the present invention is to provide a bionic hydrogel dressing and a preparation method thereof to solve the problems raised in the above background art.

[0005] To achieve the above purpose, the present invention provides the following technical solutions: A bionic hydrogel dressing includes a hydrogel matrix, polylysine, phenylalanine-based gel, propylene glycol, and deionized water, and the components are respectively in a weight ratio of: 1 - 5 parts of hydrogel matrix, 0.1 - 1 part of polylysine, 0.01 - 0.1 part of phenylalanine-based gel, 1 - 5 parts of propylene glycol, and 89 - 98 parts of deionized water.

[0006] A preparation method of a bionic hydrogel dressing includes the following steps: S1, Weigh appropriate amounts of polylysine, hydrogel matrix, and deionized water, and sequentially add the weighed polylysine and hydrogel matrix to deionized water and stir to dissolve to obtain a polylysine and hydrogel matrix mixed solution; S2, Weigh appropriate amounts of amino acid gel and propylene glycol, add the weighed amino acid gel to propylene glycol, and stir under constant temperature conditions to obtain an amino acid gel solution; S3, Mix the polylysine and hydrogel matrix mixed solution and the amino acid gel solution together and stir. After waiting for the mixed solution to be uniform, evacuate and defoam and discharge to obtain the hydrogel dressing.

[0007] As a preferred embodiment of the present invention, the hydrogel matrix comprises one or a combination of sodium carboxymethyl cellulose, sodium alginate, hydroxyethyl cellulose, polyvinyl alcohol, and hyaluronic acid; the polylysine is ε-polylysine; and the phenylalanine-based gel is a chiral phenylalanine-based gel having a biomimetic right-handed helical structure.

[0008] As a preferred embodiment of the present invention, the mass ratio of the hydrogel matrix, polylysine, phenylalanine-based gel, and deionized water is (3 - 5) : (0.5 - 1) : (0.05 - 0.1) : (1 - 2) : (92 - 96). Preferably, the mass ratio of the hydrogel matrix, polylysine, phenylalanine-based gel, and water is 4 : 0.5 : 0.05 : 2 : 93.45.

[0009] As a preferred embodiment of the present invention, the stirring and dissolving time in S1 is 4 h - 24 h, and the stirring and dissolving speed is 50 r / min - 80 r / min.

[0010] As a preferred embodiment of the present invention, the temperature of the constant temperature condition in S2 is 60°C - 90°C, the stirring duration under the constant temperature condition is 1 h - 3 h, and the stirring speed is 60 r / min - 75 r / min.

[0011] As a preferred embodiment of the present invention, the mixing and homogenizing duration in S3 is 2 h - 8 h.

[0012] As a preferred embodiment of the present invention, the hydrogel dressing obtained in S3 is used for the treatment of acute and chronic wounds. Preferably, the wounds are trauma, burns, ulcers, diabetic foot, and / or postoperative incision wounds.

[0013] Compared with the prior art, the beneficial effects of the present invention are: 1. In the present invention, by weighing appropriate amounts of polylysine, hydrogel matrix, and deionized water, the weighed polylysine and hydrogel matrix are successively added to deionized water and stirred for dissolution to obtain a mixed solution of polylysine and hydrogel matrix. Then, appropriate amounts of amino acid gel and propylene glycol are weighed, the weighed amino acid gel is added to propylene glycol, and stirred under constant temperature conditions to obtain an amino acid gel solution. Finally, the mixed solution of polylysine and hydrogel matrix and the amino acid gel solution are mixed together and stirred. After waiting for the mixed solution to be uniform, vacuum degassing is carried out and the product is discharged to obtain a hydrogel dressing. The prepared hydrogel dressing contains a chiral phenylalanine-based gel, and the chiral phenylalanine-based gel has excellent biological activity. By mimicking the wound tissue structure, it can significantly inhibit the glycosylation products in diabetic wounds, promote collagen adsorption and re-epithelialization, accelerate blood circulation reconstruction and wound healing. At the same time, the polylysine contained in the hydrogel dressing is a natural polypeptide antibacterial agent with a broad-spectrum antibacterial effect, and has a significant inhibitory effect on fungi, Gram-negative bacteria, Gram-positive bacteria, etc. Adding polylysine improves the biological activity of the hydrogel matrix and the chiral phenylalanine-based gel, and significantly enhances the wound repair effect. In addition, the hydrogel matrix and phenylalanine-based gel in the hydrogel dressing can form a double-network hydrogel with a rigid-flexible double-network interpenetrating structure, endowing the hydrogel dressing with high strength and toughness and self-healing ability. The amino group of polylysine forms hydrogen bonds with the abundant hydroxyl groups of the hydrogel matrix and phenylalanine-based gel, further strengthening its mechanical properties. By compounding the hydrogel matrix, phenylalanine-based gel, and polylysine, the prepared hydrogel dressing obtains excellent mechanical properties. Thus, the prepared hydrogel dressing has excellent biological activity, antibacterial properties, and good mechanical properties. It can not only provide a moist healing environment for wound surfaces, but also remove wound infections, accelerate blood circulation reconstruction and healing of wound surfaces. At the same time, the preparation method is simple, and a hydrogel dressing with uniform properties can be obtained through techniques such as stirring dissolution and vacuum degassing, which is beneficial for practical production applications. It solves the problems that still exist in the existing hydrogels, such as excellent biological activity, antibacterial properties, and good mechanical properties. It can not only provide a moist healing environment for wound surfaces, but also effectively solve the problems that the existing hydrogel dressings still lack structural bionics during use, are difficult to promote cell regeneration and wound tissue healing, have no antibacterial properties, and once infected by bacteria, they cannot maintain a stable environment for cell regeneration, as well as lack sufficient mechanical stability and self-healing ability. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the molecular structure of the phenylalanine-based gel of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0015] Next, in combination with the embodiments of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0016] To facilitate the understanding of the present invention, the present invention will be described more comprehensively below with reference to the relevant drawings. Several embodiments of the present invention are given. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0017] It should be noted that when an element is referred to as "fixed on" another element, it can be directly on the other element or there may also be an intermediate element. When an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are only for the purpose of illustration.

[0018] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention belongs. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0019] For the embodiments, please refer to Figure 1 , the present invention provides a technical solution: A bionic hydrogel dressing, comprising a hydrogel matrix, polylysine, phenylalanine-based gel, propylene glycol and deionized water, and the components are in weight ratios respectively: 1 - 5 parts of hydrogel matrix, 0.1 - 1 part of polylysine, 0.01 - 0.1 part of phenylalanine-based gel, 1 - 5 parts of propylene glycol, 89 - 98 parts of deionized water.

[0020] A preparation method of a bionic hydrogel dressing, comprising the following steps: S1, weigh appropriate amounts of polylysine, hydrogel matrix and deionized water, and sequentially add the weighed polylysine and hydrogel matrix into deionized water and stir to dissolve to obtain a polylysine and hydrogel matrix mixed solution; S2, weigh appropriate amounts of amino acid gel and propylene glycol, add the weighed amino acid gel into propylene glycol, and stir under constant temperature conditions to obtain an amino acid gel solution; S3. Mix the polylysine and hydrogel matrix mixed solution with the amino acid gel solution and stir. After waiting for the mixed solution to be uniform, evacuate to remove air bubbles and discharge to obtain a hydrogel dressing.

[0021] Furthermore, the hydrogel matrix includes one or a combination of sodium carboxymethylcellulose, sodium alginate, hydroxyethyl cellulose, polyvinyl alcohol, and hyaluronic acid. The polylysine is ε-polylysine, and the phenylalanine-based gel is a chiral phenylalanine-based gel with a biomimetic right-handed helical structure.

[0022] Furthermore, the mass ratio among the hydrogel matrix, polylysine, phenylalanine-based gel, and deionized water is (3 - 5):(0.5 - 1):(0.05 - 0.1):(1 - 2):(92 - 96). Preferably, the mass ratio among the hydrogel matrix, polylysine, phenylalanine-based gel, and water is 4:0.5:0.05:2:93.45.

[0023] Furthermore, the stirring and dissolving time in S1 is 4h - 24h, and the stirring and dissolving speed is 50r / min - 80r / min.

[0024] Furthermore, the temperature of the constant temperature condition in S2 is 60°C - 90°C, the stirring duration under the constant temperature condition is 1h - 3h, and the stirring speed is 60r / min - 75r / min.

[0025] Furthermore, the mixing and homogenizing duration in S3 is 2h - 8h.

[0026] Furthermore, the hydrogel dressing obtained in S3 is used for the treatment of acute and chronic wounds. Preferably, the wound is a wound of trauma, burn, ulcer, diabetic foot, and / or postoperative incision.

[0027] Weigh appropriate amounts of the hydrogel matrix, polylysine, phenylalanine-based gel, propylene glycol, and deionized water according to the mass ratio of 4:0.5:0.05:2:93.45. Add the weighed polylysine and hydrogel matrix to deionized water in sequence and stir for dissolution. The stirring and dissolving time is 24h, and the stirring and dissolving speed is 50r / min to obtain a polylysine and hydrogel matrix mixed solution. Then add the weighed amino acid gel to propylene glycol and carry out constant temperature stirring at a temperature of 60°C. The stirring duration is 1h, and the stirring speed is 60r / min to obtain an amino acid gel solution. Finally, mix the polylysine and hydrogel matrix mixed solution with the amino acid gel solution and stir. The mixing and homogenizing duration is 8h. After waiting for the mixed solution to be uniform, evacuate to remove air bubbles and discharge to obtain a hydrogel dressing.

[0028] Although embodiments of the present invention have been shown and described, those of ordinary skill in the art will understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A bionic hydrogel dressing, comprising a hydrogel matrix, polylysine, a phenylalanine-based gel, propylene glycol and deionized water, wherein the weight ratio of each component is: 1-5 parts of hydrogel matrix, 0.1-1 parts of polylysine, 0.01-0.1 parts of phenylalanine-based gel, 1-5 parts of propylene glycol, and 89-98 parts of deionized water.

2. A method for preparing a bionic hydrogel dressing, characterized in that: The following steps are involved: S1, weighing appropriate amounts of polylysine, a hydrogel matrix and deionized water, adding the weighed polylysine and the hydrogel matrix into the deionized water in sequence and stirring to dissolve, to obtain a mixed solution of polylysine and the hydrogel matrix; S2, weighing appropriate amounts of amino acid gel and propylene glycol, adding the weighed amino acid gel to the propylene glycol, and stirring at a constant temperature to obtain an amino acid gel solution; S3, mixing the polylysine and hydrogel matrix mixed solution and the amino acid gel solution together and stirring, and after the mixed solution is uniform, vacuuming, degassing and discharging to obtain a hydrogel dressing.

3. The bionic hydrogel dressing according to claim 1, characterized in that: The hydrogel matrix comprises one or a combination of sodium carboxymethyl cellulose, sodium alginate, hydroxyethyl cellulose, polyvinyl alcohol, and hyaluronic acid; the polylysine is ε-polylysine; and the phenylalanine-based gel is a chiral phenylalanine-based gel with a bionic right-handed helical structure.

4. The bionic hydrogel dressing according to claim 1, characterized in that: The mass ratio of the hydrogel matrix, polylysine, phenylalanine-based gel and deionized water is (3-5): (0.5-1): (0.05-0.1): (1-2): (92-96). Preferably, the mass ratio of the hydrogel matrix, polylysine, phenylalanine-based gel and water is 4:0.5:0.05:2:93.

45.

5. The method for preparing a bionic hydrogel dressing according to claim 1, characterized in that: The stirring and dissolving time in S1 is 4h-24h, and the stirring and dissolving speed is 50r / min-80r / min.

6. The bionic hydrogel dressing and preparation method thereof according to claim 1, characterized in that: The temperature of the constant temperature condition in S2 is 60° C.-90° C., the stirring time under the constant temperature condition is 1 h-3 h, and the stirring speed is 60 r / min-75 r / min.

7. The method for preparing a bionic hydrogel dressing according to claim 2, characterized in that: The mixing and homogenizing time in S3 is 2h-8h.

8. The method for preparing a bionic hydrogel dressing according to claim 2, characterized in that: The hydrogel dressing obtained in S3 is used for treating acute and chronic wounds. Preferably, the wound is a wound caused by trauma, burns, ulcers, diabetic foot and / or postoperative incision.

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