C-band microwave cell exposure system and use method thereof

Abstract

The invention relates to a C-band microwave cell exposure system and a use method thereof, and the C-band microwave cell exposure system comprises a first cell culture module, a second cell culture module, a radio frequency signal generator, a radio frequency power amplifier, a coupler, a radio frequency switch, a power meter and an upper computer, each of the first cell culture module and the second cell culture module comprises an experiment box, a culture dish, a corrugated irradiator and an infrared monitoring module; the ripple irradiator is used for forming a uniform electromagnetic field required by an experimental group or a control group; the radio frequency signal generator is used for generating a microwave signal with C-band frequency; the radio frequency power amplifier is used for performing power amplification on the generated microwave signal; the coupler is used for extracting part of amplified microwave signals according to a fixed proportion; the radio frequency switch is used for switching on or off of the two ripple irradiators; the power meter is used for obtaining power output by the radio frequency power amplifier; and the upper computer is used for determining the electromagnetic field intensity. The method can be widely applied to the field of C-band microwaves.

Description

Technical Field

[0001] The present invention relates to the field of C-band microwaves, and in particular to a C-band microwave cell exposure system and a use method thereof. Background Art

[0002] C-band microwaves (4-6GHz) are widely used in military and civilian fields such as radar, electronic countermeasures, communications, and aerospace and military industry. Especially with the commercial deployment of the fifth-generation mobile communication technology (5G), the importance of the C-band has increased significantly. The C-band is mainly used in 5G to provide a balanced coverage and capacity, and is an ideal frequency band for achieving wide urban coverage and indoor coverage. In these applications, human exposure to microwave radiation is inevitable, and people's general concern about the biological effects of C-band microwaves is increasing. Research on the biological effects of C-band is very important for assessing health risks and formulating corresponding safety standards.

[0003] Many studies believe that microwave exposure can affect multiple organ systems of organisms, such as sleep, cognitive impairment, decreased male fertility, myocardial cell damage, and even increase the risk of certain cancers. This has been widely discussed and concerned in the medical community. Therefore, the biological effects of electromagnetic fields within this frequency range are studied at the cellular level to reveal health effects, damage targets, and molecular mechanisms. However, there is no system in the existing technology that can achieve this goal. Summary of the invention

[0004] In view of the above problems, the object of the present invention is to provide a C-band microwave cell exposure system and a method of using the system that can reveal health effects, damage targets and molecular mechanisms.

[0005] To achieve the above-mentioned object, the present invention adopts the following technical scheme: on the one hand, a C-band microwave cell exposure system is provided, comprising a first cell culture module, a second cell culture module, a radio frequency signal generator, a radio frequency power amplifier, a coupler, a radio frequency switch, a power meter and a host computer; The first cell culture module and the second cell culture module both include an experimental box, a culture dish, a corrugated illuminator and an infrared monitoring module. Each of the experimental boxes is provided with a corresponding culture dish for placing a corresponding cell sample. The first cell culture module and the second cell culture module serve as an experimental group or a control group, respectively. Each of the experimental boxes is also provided with the corrugated illuminator and the infrared monitoring module. The corrugated irradiator is used to form a uniform electromagnetic field required by the experimental group or the control group based on the microwave signal after power amplification, and radiate the uniform electromagnetic field to the cell samples of the experimental group or the control group in a directionally manner; The infrared monitoring module is used to monitor and record in real time the temperature and electromagnetic field intensity of the exposure environment of the cell samples of the experimental group or the control group; The radio frequency signal generator is used to generate a microwave signal with a C-band frequency; The radio frequency power amplifier is used to amplify the power of the microwave signal generated by the radio frequency signal generator; The coupler is used to extract part of the amplified microwave signal at a fixed ratio; The radio frequency switch is used to switch the two corrugated illuminators on or off, and only one of the corrugated illuminators is selected to be turned on each time; The power meter is used to multiply the reading by the fixed ratio to obtain the power output by the radio frequency power amplifier; The host computer is used to control the operation of the RF signal generator, RF power amplifier and RF switch, receive data monitored by the infrared monitoring module, and determine the electromagnetic field strength according to a pre-calibrated conversion factor and the power output by the RF power amplifier.

[0006] Furthermore, the corrugated irradiator is integrally processed by CNC and includes a corrugated ring, a core tube and a coaxial feed head; The corrugated ring is a stepped horn-shaped structure with a diameter gradually increasing from bottom to top; the bottom of the corrugated ring is connected to the core tube through a flange, and the coaxial feeding head is also arranged at the lower part of the core tube.

[0007] Furthermore, the core tube is composed of five steps and a tail end smooth transition section, wherein: The total length of the core tube is 129.62 mm; the maximum outer diameter Dg of the core tube is 49.1 mm; the bottom wall thickness of the core tube is 1.5 mm, and the outer diameter is 46.1 mm; The first step height of the core tube is 5.9 mm and the length is 21.32 mm; The second step height of the core tube is 4.84 mm and the length is 16.65 mm; The third step of the core tube has a height of 3.25 mm and a length of 18.33 mm; The fourth step of the core tube has a height of 2.72 mm and a length of 21.24 mm; The fifth step of the core tube has a height of 2.5 mm and a length of 20.08 mm; The length of the smooth transition section at the tail end of the core tube is 30.5 mm, and the height ranges from 2.5 mm to 1.5 mm.

[0008] Furthermore, the length of the corrugated ring is 54.5 mm, the opening angle of the corrugated ring is 46, the number of turns of the corrugated ring is 10, the groove width of the corrugated ring is 13.6 mm, the groove wall thickness of the corrugated ring is 1.5 mm, and the groove depth of the corrugated ring is 6 mm.

[0009] Furthermore, the upper half of the core tube and the corrugated ring are of axially symmetrical structure and are processed by a common milling machine; The lower half of the core tube is a multi-step structure and is processed by a multi-axis machining center.

[0010] Furthermore, the core tube is made of copper or aluminum alloy material.

[0011] Furthermore, the first cell culture module and the second cell culture module further include culture racks, and each of the culture racks is provided with a corresponding experimental box.

[0012] On the other hand, a method for using a C-band microwave cell exposure system is provided, comprising: Placing the cell samples in the culture dishes of the first cell culture module and the second cell culture module respectively, and placing the culture dishes in corresponding experimental boxes as the experimental group and the control group; Start the host computer for initialization and self-test, and set the experimental parameters on the host computer to ensure that all parameter settings of the experimental group and the control group are the same; The host computer controls the radio frequency signal generator to generate a microwave signal of the C-band frequency, which is processed by the radio frequency power amplifier, the coupler and the power meter in sequence and then transmitted to the corrugated irradiators of the first cell culture module and the second cell culture module respectively; The RF switch switches the two corrugated irradiators on or off based on the switch control command of the host computer, and only one corrugated irradiator is selected to be turned on at a time; The corrugated irradiator of the experimental group radiates the processed microwave signal to the cell samples of the experimental group in a directionally manner, and the corrugated irradiator of the control group radiates the processed microwave signal to the cell samples of the control group in a directionally manner; During the experiment, the infrared monitoring modules of the first cell culture module and the second cell culture module monitor and record the temperature changes in the corresponding experimental box in real time and send them to the host computer for recording; The host computer determines the electromagnetic field strength based on the pre-calibrated conversion factor and the power output by the RF power amplifier sent by the power meter; After reaching the set exposure time, the host computer automatically shuts down each component, the experiment ends, and the cell samples of the experimental group and the control group are taken out for subsequent biological effect analysis.

[0013] Furthermore, the host computer controls the radio frequency signal generator to generate a microwave signal of a C-band frequency, which is processed by the radio frequency power amplifier, the coupler and the power meter in sequence and then transmitted to the corrugated irradiators of the first cell culture module and the second cell culture module respectively, including: The host computer controls the radio frequency signal generator to generate a microwave signal of C-band frequency; The radio frequency power amplifier amplifies the power of the microwave signal generated by the radio frequency signal generator; The coupler extracts part of the amplified microwave signal at a fixed ratio; The power meter multiplies the reading by the above fixed ratio to obtain the power output by the RF power amplifier and sends it to the host computer; The microwave signals after power amplification are transmitted to the corrugated irradiators of the first cell culture module and the second cell culture module respectively.

[0014] Furthermore, the host computer determines the electromagnetic field strength according to the pre-calibrated conversion factor and the power output by the radio frequency power amplifier sent by the power meter, including: The host computer multiplies the pre-calibrated conversion factor with the power output by the RF power amplifier obtained in real time by the power meter to obtain the electromagnetic field strength.

[0015] The present invention adopts the above technical solution, which has the following advantages: 1. The present invention can not only be used to analyze the effects of microwaves on cells in scientific research, but also to evaluate the possible effects of 5G and advanced WiFi technologies on human cells in daily use. The corrugated irradiator can generate a highly uniform and directional electromagnetic field in the frequency range of 4-7 GHz through its optimized geometric structure, making cell exposure experiments more accurate and reliable.

[0016] 2. Traditional open microwave cell exposure systems in the frequency band above 1 GHz usually use standard gain horns or broadband double-ridge horns. These systems often have problems of low energy efficiency and uneven field strength distribution. The present invention adopts an optimized corrugated irradiator, which has higher directionality and energy focusing, can form a uniform electromagnetic field in the cell culture area, and reduce energy radiation loss.

[0017] 3. The corrugated illuminator of the present invention is different from the traditional corrugated horn antenna. The present invention introduces specific geometric deformation to intentionally excite the illuminator to produce high-order modes, and utilizes the electric field distribution characteristics of the high-order modes to make the irradiation field intensity within the 12x12cm irradiation surface 20cm away from the illuminator mouth, especially the high-frequency band field intensity distribution more uniform.

[0018] 4. In traditional antenna design, higher-order modes are generally considered to be avoided because they lead to uneven distribution of the electromagnetic field, thereby reducing the far-field effect of the antenna and may even cause the antenna to malfunction. However, the present invention cleverly utilizes this feature. By appropriately introducing higher-order modes, the trend of electromagnetic energy becoming increasingly concentrated as the frequency increases can be destroyed, thereby improving the uniformity of the irradiated surface. Ultimately, the designed corrugated illuminator 13 achieves a relatively ideal electromagnetic field distribution and uniformity in the high-frequency band, improving the overall performance of the antenna.

[0019] 5. The present invention integrates high-precision temperature and electromagnetic field intensity monitoring instruments, and monitors the experimental environment and cell exposure status in real time through the host computer to ensure the stability and reliability of the experimental conditions.

[0020] 6. The present invention can ensure the efficiency and reliability of C-band microwave cell exposure experiments, and provide a scientific basis for evaluating the possible impact of 5G and advanced WiFi technologies on human cells in daily use.

[0021] In summary, the present invention can be widely used in the C-band microwave field. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Various other advantages and benefits will become apparent to those of ordinary skill in the art by reading the detailed description of the preferred embodiments below. The accompanying drawings are only for the purpose of illustrating the preferred embodiments and are not to be considered as limiting the present invention. Throughout the accompanying drawings, the same reference numerals are used to represent the same components. In the accompanying drawings: Figure 1 It is a schematic diagram of the overall structure of a system provided by an embodiment of the present invention; Figure 2 It is a schematic diagram of the overall structure of a corrugated illuminator provided by an embodiment of the present invention; Figure 3 It is a simplified cross-sectional schematic diagram of a core tube of a corrugated illuminator provided by an embodiment of the present invention; Figure 4 is a schematic diagram of simulation and test results using a corrugated illuminator provided by an embodiment of the present invention; Figure 5 is a schematic diagram of an irradiation platform provided by an embodiment of the present invention; Figure 6 It is a schematic diagram of the uniformity of the irradiation platform and the maximum SAR value of adherent cells at 4 GHz provided by one embodiment of the present invention; Figure 7 It is a schematic diagram of the uniformity of the illumination platform and the maximum SAR value of the suspended cells at 4 GHz provided by one embodiment of the present invention. DETAILED DESCRIPTION

[0023] The exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided in order to enable a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0024] It should be understood that the terms used herein are only for the purpose of describing specific example embodiments and are not intended to be limiting. Unless the context clearly indicates otherwise, the singular forms "one", "an" and "said" as used herein may also be meant to include plural forms. The terms "include", "comprise", "contain", and "have" are inclusive, and therefore specify the existence of stated features, steps, operations, elements and / or parts, but do not exclude the existence or addition of one or more other features, steps, operations, elements, parts, and / or combinations thereof. The method steps, processes, and operations described herein are not interpreted as necessarily requiring them to be performed in the specific order described or illustrated, unless the execution order is clearly indicated. It should also be understood that additional or alternative steps may be used.

[0025] Although the terms first, second, third, etc. can be used in the text to describe multiple elements, components, regions, layers and / or sections, these elements, components, regions, layers and / or sections should not be limited by these terms. These terms can only be used to distinguish an element, component, region, layer or section from another region, layer or section. Unless the context clearly indicates, terms such as "first", "second" and other numerical terms do not imply order or sequence when used in the text. Therefore, the first element, component, region, layer or section discussed below can be referred to as the second element, component, region, layer or section without departing from the teaching of the example embodiments.

[0026] For ease of description, spatially relative terms may be used herein to describe the relationship of one element or feature relative to another element or feature as shown in the figures, such as "inside", "outside", "inner side", "outer side", "below", "above", etc. Such spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation depicted in the figures.

[0027] At present, there is no C-band microwave cell exposure system in the prior art that can reveal health effects, damage targets and molecular mechanisms. An embodiment of the present invention provides a C-band microwave cell exposure system, including a first cell culture module, a second cell culture module, a radio frequency signal generator, a radio frequency power amplifier, a coupler, a radio frequency switch, a power meter and a host computer; the first cell culture module and the second cell culture module both include an experimental box, a culture dish, a corrugated irradiator and an infrared monitoring module, each experimental box is provided with a corresponding culture dish for placing corresponding cell samples, and the first cell culture module and the second cell culture module are respectively used as experimental groups or control groups; each experimental box is also provided with a corrugated irradiator and an infrared monitoring module; the corrugated irradiator is used to form a uniform electromagnetic field required by the experimental group or control group based on the microwave signal after power amplification, and directionally radiate to the cell samples of the experimental group or control group; the infrared monitoring module is used to The invention is used to monitor and record the temperature and electromagnetic field strength of the exposure environment of the cell samples of the experimental group or the control group in real time; the radio frequency signal generator is used to generate a microwave signal of C-band frequency; the radio frequency power amplifier is used to amplify the microwave signal generated by the radio frequency signal generator; the coupler is used to extract the amplified part of the microwave signal according to a fixed ratio; the radio frequency switch is used to switch the opening or closing of the two corrugated irradiators, and only one corrugated irradiator is selected to be turned on each time; the power meter is used to multiply the reading by the above fixed ratio to obtain the power output by the radio frequency power amplifier; the host computer is used to control the operation of the radio frequency signal generator, the radio frequency power amplifier and the radio frequency switch, receive the data monitored by the infrared monitoring module, and determine the electromagnetic field strength according to the pre-calibrated conversion factor and the power output by the radio frequency power amplifier. The present invention can not only be used to analyze the effect of microwaves on cells in scientific research, but also to evaluate the possible effects of 5G and advanced WiFi technologies on human cells in daily use. The corrugated horn antenna design adopted by the system can generate a highly uniform and directional electromagnetic field in the frequency range of 4-7 GHz through its optimized geometric structure, making the cell exposure experiment more accurate and reliable.

[0028] Example 1 like Figure 1 As shown, this embodiment provides a C-band microwave cell exposure system, including a first cell culture module 1, a second cell culture module 2, a radio frequency signal generator 3, a radio frequency power amplifier 4, a coupler 5, a radio frequency switch 6, a power meter 7 and a host computer 8, wherein the first cell culture module 1 and the second cell culture module 2 both include a culture rack, an experimental box 11, a culture dish 12, a corrugated illuminator 13 and an infrared monitoring module 14.

[0029] The culture racks of the first cell culture module 1 and the second cell culture module 2 are both provided with corresponding experimental boxes 11, and each experimental box 11 is provided with corresponding culture dishes 12 for placing corresponding cell samples. The first cell culture module 1 is the experimental group and the second cell culture module 2 is the control group, or the first cell culture module 1 is the control group and the second cell culture module 2 is the experimental group. Each experimental box 11 is also provided with a corrugated irradiator 13 and an infrared monitoring module 14. The corrugated irradiator 13 is used to form a uniform electromagnetic field required by the experimental group or the control group based on the microwave signal after power amplification, and radiate it directionally to the cell samples of the experimental group or the control group. The infrared monitoring module 14 is used to monitor and record the temperature and electromagnetic field strength of the exposure environment of the cell samples of the experimental group or the control group in real time.

[0030] The host computer 8 is connected to the infrared monitoring module 14 and the RF signal generator 3 respectively. The RF signal generator 3 is connected to the RF switch 6 through the RF power amplifier 4 and the coupler 5 in turn. The RF switch 6 is connected to the two ripple irradiators 13 respectively. The coupler 5 is also connected to the power meter 7.

[0031] The RF signal generator 3 is used to generate a microwave signal with a C-band frequency based on the signal control command of the host computer 8, and supports the generation of complex modulation signals such as AM / FM / PM / 4G / 5G / WiFi.

[0032] The RF power amplifier 4 is used to amplify the power of the microwave signal generated by the RF signal generator 3 based on the power amplifier control command of the host computer 8 .

[0033] The coupler 5 is used to extract part of the amplified microwave signal according to a fixed ratio.

[0034] The radio frequency switch 6 is used to switch the two corrugated irradiators 13 on or off based on the switch control command of the host computer 8, and only one corrugated irradiator 13 is selected to be turned on each time.

[0035] The power meter 7 is used to multiply the reading by the above fixed ratio to obtain the power output by the RF power amplifier 4 and send it to the host computer 8 .

[0036] The host computer 8 is used to control the operation of the RF signal generator 3, the RF power amplifier 4 and the RF switch 6, receive data monitored by the infrared monitoring module 14, and determine the electromagnetic field strength according to the pre-calibrated conversion factor and the power output by the RF power amplifier 4.

[0037] In a preferred embodiment, the inner walls of the experimental boxes 11 of the first cell culture module 1 and the second cell culture module 2 are both provided with absorbing materials 15 .

[0038] In a preferred embodiment, Figure 2As shown, the corrugated illuminator 13 is made of CNC integrated processing, including a corrugated ring 131, a core tube 132 and a coaxial feed head 133, wherein the corrugated ring 131 is a stepped horn-shaped structure with a diameter gradually increasing from bottom to top. The bottom of the corrugated ring 131 is connected to the core tube 132 through a flange, and a coaxial feed head 133 is also provided at the lower part of the core tube 132. The corrugated illuminator 13 of the present invention is different from the traditional corrugated horn antenna. The present invention introduces specific geometric deformations to intentionally excite the illuminator to produce high-order modes, and utilizes the electric field distribution characteristics of the high-order modes to make the irradiation field strength in the 12x12cm irradiation surface (i.e., the plane where the culture dish 12 is located) 20cm away from the irradiator mouth, especially the high-frequency band field strength distribution, more uniform.

[0039] In a preferred embodiment, the corrugated ring 131 is a key part in the design of the corrugated illuminator 13 of this embodiment. The periodic structure introduced by it can effectively control the propagation characteristics of electromagnetic waves by strengthening the current resonance. Through precise optimization design, the groove width and depth of the corrugated ring 131 can be adjusted to achieve the following goals: 1) Improve gain: optimize the corrugated structure, improve the gain of the corrugated illuminator 13, and concentrate the radiation energy in the main lobe direction. 2) Reduce side lobes: by adjusting the periodic structure of the corrugation, reduce the side lobe level, reduce interference and noise. 3) Improve matching: accurately design the geometric parameters of the corrugation to make the impedance matching between the corrugated illuminator 13 and the microwave signal system (i.e., the RF signal generator 3 and the RF power amplifier 4) better, thereby reducing reflections and standing waves. The core tube 132 of the corrugated illuminator 13 is the core part of the corrugated illuminator 13, and its main function is to guide and modulate the propagation of electromagnetic waves. The design of the core tube 132 needs to consider the following aspects: 1) Structural optimization: The core tube 132 is designed as a multi-step structure, which can effectively control the phase and amplitude distribution of electromagnetic waves and improve the radiation efficiency of the antenna. 2) Material selection: The core tube 132 uses a high conductivity and low loss material, such as copper or aluminum alloy, to reduce energy loss and thermal effects.

[0040] In a preferred embodiment, Figure 3As shown, the core tube 132 consists of five steps and a smooth transition section at the tail end, and the total length of the core tube 132 is 129.62 mm, wherein the maximum outer diameter Dg of the core tube 132 is 49.1 mm; the bottom wall thickness of the core tube 132 is 1.5 mm, and the outer diameter is 46.1 mm; the first step of the core tube 132 is 5.9 mm high and 21.32 mm long, and the middle part is a standard N-type female connector; the second step of the core tube 132 is 4.84 mm high and 16.65 mm long; the third step of the core tube 132 is 3.25 mm high and 18.33 mm long; the fourth step of the core tube 132 is 2.72 mm high and 21.24 mm long; the fifth step of the core tube 132 is 2.5 mm high and 20.08 mm long; the tail end smooth transition section of the core tube 132 is 30.5 mm long, and the height ranges from 2.5 mm to 1.5 mm.

[0041] In a preferred embodiment, the length Lg of the corrugated ring 131 is 54.5 mm, the opening angle Theta of the corrugated ring 131 is 46, the number of turns N of the corrugated ring 131 is 10, the groove width Sd of the corrugated ring 131 is 13.6 mm, the groove wall thickness Wr of the corrugated ring 131 is 1.5 mm, and the groove depth Ws of the corrugated ring 131 is 6 mm.

[0042] In a preferred embodiment, the processing process of the corrugated illuminator 13 is as follows: 1) Processing of the upper part of the corrugated irradiator 13: Structural features: The upper half (linear gradient portion) of the core tube 132 and the corrugated ring 131 are both simple axisymmetric structures.

[0043] Processing method: Ordinary milling machines can be used for processing. Ordinary milling machines are efficient and economical, and are suitable for processing these regular symmetrical structures.

[0044] 2) Processing of the lower part of the corrugated irradiator 13: Structural features: The lower half of the core tube 132 is a multi-step structure with high complexity.

[0045] Processing method: Use a high-end multi-axis machining center for processing. The multi-axis machining center can accurately control the movement trajectory of the tool to ensure the processing accuracy and surface quality of complex geometric shapes.

[0046] 3) Overall assembly: Connection method: After processing, the upper and lower parts are connected together through the designed flange. The flange connection must ensure the strength and sealing of the structure to avoid electromagnetic wave leakage and mechanical looseness.

[0047] Inspection and testing: After assembly, strict inspection and electrical performance testing are required to ensure that the design indicators of the corrugated illuminator 13 are achieved.

[0048] The corrugated illuminator 13 obtained through the above-mentioned processing can significantly improve efficiency and performance. The optimized design of the corrugated ring 131 and the precision-machined core tube 132 structure not only improve the gain and directivity of the corrugated illuminator 13, but also reduce the side lobe level and energy loss. In particular, by reasonably designing the transition structure of the coaxial to waveguide and introducing the structural deformation of the high-order mode, efficient impedance matching and signal transmission can be achieved in a wide frequency band. After simulation and testing, the corrugated illuminator 13 of this embodiment has significantly improved irradiation efficiency and uniformity, such as Figure 4 shown.

[0049] In a preferred embodiment, this embodiment uses electromagnetic simulation software (such as CST, HFSS, etc.) for simulation optimization to adjust the length, diameter and transition structure of the core tube 132 of the corrugated illuminator 13 to ensure that the core tube 132 achieves good impedance matching and efficient transmission within the designed frequency band.

[0050] In a preferred embodiment, the infrared monitoring module 14 may adopt a precision infrared imager.

[0051] The effectiveness of the C-band microwave cell exposure system of the present invention is described in detail below through specific examples: In order to verify the effectiveness of the C-band microwave cell exposure system of the present invention, a prototype was designed and manufactured. In the experiment, different types of cell samples were placed in the uniform field area of ​​the corrugated irradiator 13, and different exposure parameters such as power, frequency and time were set by the host computer 8.

[0052] Set the output frequency of the RF signal generator 3 to 4 GHz; adjust the output power of the RF signal generator 3 so that the reading of the power meter 7 is 40-CO(4)+L(4)dBm, that is, the input power of the corrugated illuminator 13 is 40 dBm, read the total effective value of the field strength of the field strength probe (the component used for initial and regular calibration of the field strength), this reading is the field strength value at point (0,0) in the irradiation plane, move the field strength probe in sequence at intervals of 1.5 cm in the 12x12 cm plane, and read the total effective value of the field strength of the field strength probe, and simulate to obtain the field strength distribution value of the irradiation surface under this frequency. At 20 cm from the irradiator mouth, within the 12 cmx12 cm irradiation area, the maximum and minimum deviations of the field strength values ​​corresponding to the culture dish 12 in the irradiation plane detected at 4 GHz are no more than 35%, such as Figure 5 shown.

[0053] Adherent cells: Import the electric field data of the test into SEMCAD, set the relative dielectric constant and conductivity of the culture, and use DMEM culture medium for the verification project. The conductivity and dielectric constant are 0.6mS / cm and 80 respectively. Set the culture medium volume to 0.5mL, and simulate to obtain the maximum SAR peak value of 100.773W / kg. The SAR non-uniformity is less than 20%. Figure 6 shown.

[0054] Suspended cells: irradiate the 35mL culture dish 12 on the tray. When the input power of the corrugated irradiator 13 is 10W and the input frequency is 4GHz, import the tested electric field data into SEMCAD, set the relative dielectric constant and conductivity of the culture, and use DMEM culture medium for the verification project. The conductivity and dielectric constant are 0.6mS / cm and 80 respectively. Set the culture medium volume to 0.5mL, and simulate the maximum average SAR of suspended cells: 122.769 W / kg, and the SAR inhomogeneity is less than 20%, such as Figure 7 shown.

[0055] The experimental results show that compared with the traditional horn antenna, the corrugated illuminator 13 of the present invention can significantly improve energy efficiency, ensure the uniformity of field strength and the repeatability of the experiment. In addition, the system's integrated real-time monitoring and data recording functions can accurately record all key parameters during the exposure process, such as electromagnetic field strength, frequency, exposure time, and ambient temperature, thereby providing researchers with a large amount of reliable data.

[0056] Example 2 This embodiment provides a method for using a C-band microwave cell exposure system, comprising the following steps: 1) Cell samples are placed in the culture dishes 12 of the first cell culture module 1 and the second cell culture module 2 respectively, and the culture dishes 12 are placed in the corresponding experimental boxes 11 as the experimental group and the control group.

[0057] 2) Start the host computer 8 to perform initialization and self-test to ensure that all modules of the host computer 8 are working properly.

[0058] 3) Set the experimental parameters on the host computer 8 to ensure that all parameter settings of the experimental group and the control group are the same to ensure the fairness and scientificity of the experiment.

[0059] Specifically, the parameters to be set include frequency, power, exposure time and temperature, etc.

[0060] 4) The host computer 8 controls the RF signal generator 3 to generate a microwave signal of C-band frequency, which is processed by the RF power amplifier 4, the coupler 5 and the power meter 7 in sequence and then transmitted to the corrugated irradiator 13 of the first cell culture module 1 and the second cell culture module 2 respectively, specifically: 4.1) The host computer 8 controls the RF signal generator 3 to generate a microwave signal with a C-band frequency.

[0061] 4.2) The RF power amplifier 4 amplifies the power of the microwave signal generated by the RF signal generator 3 .

[0062] 4.3) Coupler 5 extracts part of the amplified microwave signal at a fixed ratio.

[0063] 4.4) The power meter 7 multiplies the reading by the above fixed ratio to obtain the power output by the RF power amplifier 4 and sends it to the host computer 8.

[0064] 4.5) The microwave signal after power amplification is transmitted to the corrugated irradiator 13 of the first cell culture module 1 and the second cell culture module 2 respectively.

[0065] 5) The radio frequency switch 6 switches the two corrugated irradiators 13 on or off based on the switch control command of the host computer 8, and only one corrugated irradiator 13 is selected to be turned on each time.

[0066] 6) The corrugated irradiator 13 of the experimental group directional radiates the processed microwave signal to the cell samples of the experimental group, and the corrugated irradiator 13 of the control group directional radiates the processed microwave signal to the cell samples of the control group.

[0067] 7) During the experiment, the infrared monitoring modules 14 of the first cell culture module 1 and the second cell culture module 2 monitor and record the temperature changes in the corresponding experimental box 11 in real time and send them to the host computer 8 for recording.

[0068] 8) The host computer 8 determines the electromagnetic field strength according to the pre-calibrated conversion factor and the power output by the radio frequency power amplifier 4 sent by the power meter 7 to ensure the stability of the experimental environment.

[0069] Specifically, the host computer 8 multiplies the pre-calibrated conversion factor by the power output by the radio frequency power amplifier 4 acquired in real time by the power meter 7 to obtain the electromagnetic field strength.

[0070] 9) After the set exposure time is reached, the host computer 8 automatically shuts down each component, the experiment ends, and the cell samples of the experimental group and the control group are taken out for subsequent biological effect analysis.

[0071] 10) Researchers can analyze the data recorded by the host computer 8 and the cell samples taken out, including the impact of electromagnetic fields on cells, temperature changes, etc., and draw scientific and reasonable conclusions.

[0072] The above embodiments are only used to illustrate the present invention, wherein the structure, connection mode and manufacturing process of each component may be changed. Any equivalent transformations and improvements based on the technical solution of the present invention should not be excluded from the protection scope of the present invention.

Claims

1. A C-band microwave cell exposure system, characterized in that: It includes a first cell culture module, a second cell culture module, a radio frequency signal generator, a radio frequency power amplifier, a coupler, a radio frequency switch, a power meter and a host computer; The first cell culture module and the second cell culture module both include an experimental box, a culture dish, a corrugated illuminator and an infrared monitoring module. Each of the experimental boxes is provided with a corresponding culture dish for placing a corresponding cell sample. The first cell culture module and the second cell culture module serve as an experimental group or a control group, respectively. Each of the experimental boxes is also provided with the corrugated illuminator and the infrared monitoring module. The corrugated irradiator is used to form a uniform electromagnetic field required by the experimental group or the control group based on the microwave signal after power amplification, and radiate the uniform electromagnetic field to the cell samples of the experimental group or the control group in a directionally manner; The infrared monitoring module is used to monitor and record in real time the temperature and electromagnetic field intensity of the exposure environment of the cell samples of the experimental group or the control group; The radio frequency signal generator is used to generate a microwave signal with a C-band frequency; The radio frequency power amplifier is used to amplify the power of the microwave signal generated by the radio frequency signal generator; The coupler is used to extract part of the amplified microwave signal at a fixed ratio; The radio frequency switch is used to switch the two corrugated illuminators on or off, and only one of the corrugated illuminators is selected to be turned on each time; The power meter is used to multiply the reading by the fixed ratio to obtain the power output by the radio frequency power amplifier; The host computer is used to control the operation of the RF signal generator, RF power amplifier and RF switch, receive data monitored by the infrared monitoring module, and determine the electromagnetic field strength according to a pre-calibrated conversion factor and the power output by the RF power amplifier.

2. A C-band microwave cell exposure system as claimed in claim 1, characterized in that: The corrugated irradiator is made by CNC integrated processing, including a corrugated ring, a core tube and a coaxial feed head; The corrugated ring is a stepped horn-shaped structure with a diameter gradually increasing from bottom to top; the bottom of the corrugated ring is connected to the core tube through a flange, and the coaxial feeding head is also arranged at the lower part of the core tube.

3. A C-band microwave cell exposure system as claimed in claim 2, characterized in that: The core tube is composed of five steps and a smooth transition section at the tail end, wherein: The total length of the core tube is 129.62 mm; the maximum outer diameter Dg of the core tube is 49.1 mm; the bottom wall thickness of the core tube is 1.5 mm, and the outer diameter is 46.1 mm; The first step height of the core tube is 5.9 mm and the length is 21.32 mm; The second step height of the core tube is 4.84 mm and the length is 16.65 mm; The third step of the core tube has a height of 3.25 mm and a length of 18.33 mm; The fourth step of the core tube has a height of 2.72 mm and a length of 21.24 mm; The fifth step of the core tube has a height of 2.5 mm and a length of 20.08 mm; The length of the smooth transition section at the tail end of the core tube is 30.5 mm, and the height ranges from 2.5 mm to 1.5 mm.

4. A C-band microwave cell exposure system as claimed in claim 2, characterized in that: The length of the corrugated ring is 54.5 mm, the opening angle of the corrugated ring is 46, the number of turns of the corrugated ring is 10, the groove width of the corrugated ring is 13.6 mm, the groove wall thickness of the corrugated ring is 1.5 mm, and the groove depth of the corrugated ring is 6 mm.

5. A C-band microwave cell exposure system as claimed in claim 2, characterized in that: The upper half of the core tube and the corrugated ring are of axisymmetric structure and are processed by a common milling machine; The lower half of the core tube is a multi-step structure and is processed by a multi-axis machining center.

6. A C-band microwave cell exposure system as claimed in claim 1, characterized in that: The core tube is made of copper or aluminum alloy.

7. A C-band microwave cell exposure system as claimed in claim 1, characterized in that: The first cell culture module and the second cell culture module further include culture racks, and each of the culture racks is provided with a corresponding experimental box.

8. A method for using the C-band microwave cell exposure system according to any one of claims 1 to 7, characterized in that: include: Placing the cell samples in the culture dishes of the first cell culture module and the second cell culture module respectively, and placing the culture dishes in corresponding experimental boxes as the experimental group and the control group; Start the host computer for initialization and self-test, and set the experimental parameters on the host computer to ensure that all parameter settings of the experimental group and the control group are the same; The host computer controls the radio frequency signal generator to generate a microwave signal of the C-band frequency, which is processed by the radio frequency power amplifier, the coupler and the power meter in sequence and then transmitted to the corrugated irradiators of the first cell culture module and the second cell culture module respectively; The RF switch switches the two corrugated irradiators on or off based on the switch control command of the host computer, and only one corrugated irradiator is selected to be turned on at a time; The corrugated irradiator of the experimental group radiates the processed microwave signal to the cell samples of the experimental group in a directionally manner, and the corrugated irradiator of the control group radiates the processed microwave signal to the cell samples of the control group in a directionally manner; During the experiment, the infrared monitoring modules of the first cell culture module and the second cell culture module monitor and record the temperature changes in the corresponding experimental box in real time and send them to the host computer for recording; The host computer determines the electromagnetic field strength based on the pre-calibrated conversion factor and the power output by the RF power amplifier sent by the power meter; After reaching the set exposure time, the host computer automatically shuts down each component, the experiment ends, and the cell samples of the experimental group and the control group are taken out for subsequent biological effect analysis.

9. The method for using the C-band microwave cell exposure system according to claim 8, characterized in that: The host computer controls the radio frequency signal generator to generate a microwave signal with a C-band frequency, which is processed by the radio frequency power amplifier, the coupler and the power meter in sequence and then transmitted to the corrugated irradiators of the first cell culture module and the second cell culture module respectively, including: The host computer controls the radio frequency signal generator to generate a microwave signal of C-band frequency; The radio frequency power amplifier amplifies the power of the microwave signal generated by the radio frequency signal generator; The coupler extracts part of the amplified microwave signal at a fixed ratio; The power meter multiplies the reading by the above fixed ratio to obtain the power output by the RF power amplifier and sends it to the host computer; The microwave signals after power amplification are transmitted to the corrugated irradiators of the first cell culture module and the second cell culture module respectively.

10. The method for using the C-band microwave cell exposure system according to claim 8, characterized in that: The host computer determines the electromagnetic field strength according to the pre-calibrated conversion factor and the power output by the radio frequency power amplifier sent by the power meter, including: The host computer multiplies the pre-calibrated conversion factor with the power output by the RF power amplifier obtained in real time by the power meter to obtain the electromagnetic field strength.

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