Application of catechin in promoting growth and development of rice and / or increasing yield of rice

Abstract

The invention belongs to the technical field of rice cultivation, and particularly relates to application of catechin in promoting rice growth and development and / or increasing rice yield. The invention finds that catechin has the function of a biostimulant, and can effectively promote the growth and development of rice and increase the yield of rice. Planting tests show that by applying a proper amount of catechin to rice through root irrigation, direct field application along with base fertilizer or foliage spraying, growth of the overground part of the rice can be remarkably promoted, growth of the underground part of the rice can be promoted, the nitrogen accumulation amount of the rice is increased, the tiller number of the rice is increased, the yield of the rice is increased, and the effect is excellent.

Description

Technical Field

[0001] The present invention belongs to the technical field of rice cultivation, and particularly relates to the application of catechin in promoting the growth and development of rice and / or increasing the yield of rice. Background Art

[0002] Rice is an important food crop, and nitrogen is a key nutrient limiting the yield of rice. However, the current situation of excessive nitrogen fertilizer input and low nitrogen use efficiency seriously restricts the sustainable development of rice production. The root system is an important organ for rice to absorb nitrogen. The morphological changes of the root system during the seedling stage of rice have an impact on processes such as flowering, pollination, fertilization, and seed setting, and are related to the final yield of rice. Well-developed roots are also a sign of strong rice seedlings, and cultivating strong rice seedlings is an important guarantee for high and stable yields of rice. In actual production, the natural environmental conditions of the seedbed are often restricted by time and space. To ensure the quality of the seedlings, growers often apply excessive chemical fertilizers, resulting in excessive growth of the seedlings and poor root development, affecting the rooting of rice. Therefore, it is particularly important to find a new regulatory substance that can promote the growth and development of rice.

[0003] Biostimulants have various biological sources, including plant sources, animal sources, and microbial sources. When applied to plant leaves and rhizospheres, they are beneficial to plants in absorbing nutrients, resisting abiotic stresses, and improving quality, etc., and can exert the growth potential of plants in conventional cultivation, regardless of their own nutrient components. Currently, the research on biostimulants still mainly focuses on complex mixtures, and the exploration, identification, and application of their active ingredients have broad prospects. Therefore, exploring the growth-promoting effects of biological metabolic extracts on plants is an effective way to improve the efficient use of resources and the sustainable development of agriculture.

[0004] Flavonoids are widely distributed. Most of them are flavonoid derivatives containing hydroxyl groups and belong to plant secondary metabolites, which have extremely high application values in the fields of chemistry, pharmacy, and biology. In the agricultural field, flavonoids are important molecules that affect plant growth and development, and resist biotic and abiotic stresses, and are widely used in aspects such as plant growth promotion, quality improvement, stress resistance improvement, antibacterial, and antioxidant. Currently, known flavonoid natural plant growth regulators applied in agricultural production include quercetin, apigenin, and puerarin, etc., but whether the tea tree secondary metabolite catechin can be used as a plant growth regulator has not been reported. Summary of the Invention

[0005] The purpose of the present invention is to provide the application of catechin in promoting the growth and development of rice and / or increasing the yield of rice. Catechin can effectively promote the growth and development of rice and increase the yield of rice.

[0006] The present invention provides the application of catechin in promoting the growth and development of rice and / or increasing the yield of rice.

[0007] Preferably, the application of promoting rice growth and development includes one or more of promoting the growth of the above-ground part of rice, promoting the growth of the underground part of rice, increasing the nitrogen accumulation in the above-ground part of rice, increasing the nitrogen accumulation in the rice roots, and increasing the tiller number of rice.

[0008] Preferably, promoting the growth of the above-ground part of rice includes one or more of increasing the plant height, leaf area, total chlorophyll content, and above-ground biomass of rice.

[0009] Preferably, promoting the growth of the underground part of rice includes one or more of increasing the total volume, total area, and biomass of rice roots.

[0010] Preferably, the application method of catechin includes one or more of root irrigation, foliar spraying, and broadcasting.

[0011] Preferably, catechin is applied as a biostimulant.

[0012] The present invention also provides a method for promoting rice growth and development and / or increasing rice yield, including: applying catechin to rice.

[0013] Preferably, the application method includes root irrigation of rice seedlings; the application concentration of catechin is 5-50 mg / L.

[0014] Preferably, the application method includes broadcasting; the application amount of catechin is 0.5-4 kg / mu; the broadcasting includes broadcasting catechin together with the basal fertilizer.

[0015] Preferably, the application method includes foliar spraying; the application amount of catechin is 1-5 kg / mu / time, and it is applied 2 times in total; the two application periods include the early tillering stage and the late tillering stage of rice.

[0016] Beneficial effects:

[0017] The present invention provides the application of catechin in promoting rice growth and development and / or increasing rice yield. The present invention discovers that catechin has the function of a biostimulant, which can effectively promote rice growth and development and increase rice yield. Through planting experiments, it is found that applying an appropriate amount of catechin to rice by root irrigation, direct field application together with the basal fertilizer, or foliar spraying can significantly promote the growth of the above-ground part of rice, promote the growth of the underground part of rice, increase the nitrogen accumulation in rice, increase the tiller number of rice, and increase the yield of rice, and the effect is excellent. Description of the Drawings

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the embodiments.

[0019] Figure 1The chemical structural formula of catechin used in the present invention;

[0020] Figure 2 The phenotypes of rice seedlings after irrigation with different concentrations of catechin irrigation solution in Example 1 of the present invention; among them, C 0 、C 5 、C 10 、C 20 、C 50 represent that the added concentrations of catechin are 0, 5 mg / L, 10 mg / L, 20 mg / L, and 50 mg / L respectively;

[0021] Figure 3 The effects of irrigation with different concentrations of catechin irrigation solution on the nitrogen accumulation in rice seedlings in Example 1 of the present invention. Detailed implementation mode

[0022] The present invention provides the application of catechin in promoting the growth and development of rice and / or increasing the yield of rice.

[0023] The CAS number of the catechin described in the present invention is 7295-85-4, and the structural formula is as Figure 1 shown. As an implementation mode, the promotion of rice growth and development described in the present invention includes the application in one or more of promoting the above-ground growth of rice, promoting the underground growth of rice, increasing the nitrogen accumulation in the above-ground part of rice, increasing the nitrogen accumulation in the rice roots, and increasing the tiller number of rice. As an implementation mode, the promotion of the above-ground growth of rice includes increasing one or more of the plant height, leaf area, total chlorophyll content, and above-ground biomass of rice. As an implementation mode, the promotion of the underground growth of rice includes increasing one or more of the total volume, total area, and biomass of rice roots. As an implementation mode, the increase in rice yield can be an increase in grain yield.

[0024] As an implementation mode, the application method of the catechin can be one or more of irrigation, foliar spraying, and topdressing; as another implementation mode, the application method of the catechin can be irrigation, foliar spraying, or topdressing.

[0025] As an implementation mode, the catechin is applied as a biostimulant.

[0026] The present invention also provides a method for promoting the growth and development of rice and / or increasing the yield of rice, including: applying catechin to rice.

[0027] As an implementation method, the application method includes root irrigation treatment of rice seedlings; the application concentration of catechin is 5-50 mg / L; as another implementation method, the application concentration of catechin can be 5 mg / L, 10 mg / L, 20 mg / L or 50 mg / L. As an implementation method, when catechin is applied by root irrigation, the solvent is water. In a specific embodiment, the first root irrigation is carried out on the 3rd day after the emergence of rice seedlings, and the root irrigation is carried out every 7 days. The amount of root irrigation each time is 100 mL / cavity, and the root irrigation is carried out 4 times in total; two rice plants are planted in each cavity. The present invention formulates catechin into a catechin solution for root irrigation of rice seedling raising, which can significantly promote the growth of rice roots, improve the rice biomass, the total chlorophyll content of rice and the nitrogen accumulation amount of rice, and achieve the effect of raising strong seedlings.

[0028] As an implementation method, the application method can be broadcasting; as another implementation method, the broadcasting is to broadcast the catechin together with the base fertilizer, that is, to mix the catechin with the base fertilizer and then broadcast it. As an implementation method, the application amount of catechin is 0.5-4 kg / mu; as another implementation method, the application amount of catechin can be 0.5 kg / mu, 1 kg / mu, 2 kg / mu, 3 kg / mu or 4 kg / mu.

[0029] As an implementation method, the application method can be foliar spraying. As an implementation method, the application amount of catechin is 1-5 kg / mu; as another implementation method, the application amount of catechin can be 1 kg / mu, 2 kg / mu, 3 kg / mu, 4 kg / mu or 5 kg / mu. As an implementation method, the number of times of catechin application can be 2 times; as another implementation method, the two application periods can be the early tillering stage and the late tillering stage of rice. The present invention has no strict limit on the boundary between the early tillering stage and the late tillering stage, and those skilled in the art can determine it according to professional technical conventions. As an implementation method, when the present invention carries out the foliar spraying, catechin is mixed and dissolved with an equal mass of water to prepare a catechin solution and then sprayed.

[0030] The present invention directly applies the catechin in the field, that is, broadcasting together with the base fertilizer or foliar spraying, which can significantly promote rice tillering, improve the plant height, nitrogen accumulation amount and yield of rice.

[0031] In order to further illustrate the present invention, the technical effects provided by the present invention will be described in detail below in conjunction with the drawings and embodiments, but they cannot be understood as a limitation to the protection scope of the present invention.

[0032] The CAS number of catechin used in the following examples is 7295-85-4, and the structural formula is as Figure 1 shown.

[0033] Example 1

[0034] Use of catechin for root irrigation in promoting root growth and nitrogen utilization of rice seedlings, the steps are as follows:

[0035] Rice variety: Heliangyou 332;

[0036] Rice seedling raising substrate: purchased from Sichuan Hainadi Agricultural Technology Co., Ltd., the product name or product number is "Hainadi Special Rice Seedling Substrate", the main components are peat, vermiculite and yellow mud, and the pH value range is 5.5 - 7.5;

[0037] Catechin solution: Catechin was dissolved in distilled water to prepare 5 catechin root irrigation solutions with different concentrations, namely 0mg / L, 5mg / L, 10mg / L, 20mg / L and 50mg / L;

[0038] Catechin application method: root irrigation;

[0039] Implementation steps: Fill the rice seedling raising substrate into the rice seedling raising tray, irrigate with distilled water until the substrate is in a moist state. After the rice is normally soaked and germinated, sow 2 seeds in each hole of the seedling raising tray, and then gently cover a layer of substrate on the surface of the rice seeds, and irrigate with distilled water until it is in a moist state. On the 3rd day after the rice seedlings emerge, irrigate with different concentrations of catechin root irrigation solution every 7 days, the irrigation amount is 100 mL per hole, and irrigate the roots 4 times in total to ensure that the irrigation amounts of each treatment are the same. The irrigation time is about 8 pm to reduce evaporation loss.

[0040] When the rice seedlings emerge for 30 days and grow to the three-leaf and one-heart stage, take 10 rice plants from each treatment to measure the plant height, leaf area and chlorophyll content of the rice; use a root scanner to extract the rice root parameters. Another 10 rice plants are divided into above-ground parts and roots, dried, weighed, ground and then the nitrogen nutrient content is measured.

[0041] The results show that catechin root irrigation can optimize the stem and leaf parameters and root parameters of rice (Table 1). When the concentration of the catechin root irrigation solution is 20mg / L, the growth of rice seedlings reaches a relatively optimal level ( Figure 2 ). Compared with the 0mg / L catechin root irrigation solution, 5 - 50mg / L catechin root irrigation can increase the plant height of rice by 12.8% - 16.0%, leaf area by 23.9% - 46.1%, chlorophyll content by 226.1% - 320.1%, above-ground biomass by 50.7% - 81.6%, total root volume by 43.7% - 68.8%, total root surface area by 55.4% - 73.4%, and root biomass by 69.5% - 247.6% (Table 1). In addition, different concentrations of catechin root irrigation solution increase the nitrogen accumulation in the above-ground part and roots of rice by 73.9% - 106.0% and 77.6% - 285.7% respectively ( Figure 3 ).

[0042] Table 1 Effects of Root Drenching with Catechin Solutions at Different Concentrations on the Growth of Rice Seedlings

[0043]

[0044] Example 2

[0045] Use of Catechin Applied with Base Fertilizer in Increasing the Tillering Number and Yield of Paddy Rice in the Field, the steps are as follows:

[0046] Rice variety: Heliangyou 332;

[0047] Application method of catechin: Applied with base fertilizer, set 5 application rates, which are 0.5 kg / mu, 1 kg / mu, 2 kg / mu, 3 kg / mu and 4 kg / mu respectively. The experiment without applying catechin (i.e., applying 0 kg / mu) was used as a blank control.

[0048] Implementation steps: The rice was transplanted by seedling raising, the transplanting density was 14,000 hills / mu, the fertilizer application rates were 12 kg / mu of nitrogen fertilizer, 4 kg / mu of phosphate fertilizer, and 5 kg / mu of potassium fertilizer; the nitrogen fertilizer was applied according to 50% base fertilizer + 30% tillering fertilizer + 20% panicle fertilizer, the phosphate fertilizer was applied once as base fertilizer, and the potassium fertilizer was applied according to 2 / 3 base fertilizer + 1 / 3 panicle fertilizer. The test fertilizer varieties were urea (46% N), superphosphate (12% P 2 O 5 ) and potassium chloride (60% K 2 O) and were applied as nitrogen fertilizer, phosphate fertilizer and potassium fertilizer respectively.

[0049] Before transplanting the rice, the base fertilizer and catechin were mixed evenly and then applied. At the late tillering stage of the rice, 5 random 1 m 2 quadrats were selected for each treatment to investigate the tillering number and plant height of the rice. In addition, at the time of mature harvest of the rice, 5 random 1 m 2 quadrats were selected for each treatment to measure the nitrogen nutrient content of the rice, and the yield was counted according to the actual production.

[0050] The results showed that applying catechin with base fertilizer could increase the tillering number, biomass and yield of rice. In the range of application rates from 0.5 to 4 kg / mu, it could increase the plant height of rice by 2.3% - 12.9%, the tillering number by 9.5% - 52.4%, the grain yield by 3.7% - 20.7% and the above-ground nitrogen accumulation by 7.4% - 49.1% (Table 2).

[0051] Table 2 Effects of Applying Different Amounts of Catechin with Base Fertilizer on the Yield of Rice

[0052]

[0053]

[0054] Example 3

[0055] Use of catechin foliar spraying in increasing tiller number and yield of paddy rice in the field, the steps are as follows:

[0056] Rice variety: Heliangyou 332;

[0057] Application method of catechin: foliar spraying, set 5 application rates, which are 1 kg / mu / time, 2 kg / mu / time, 3 kg / mu / time, 4 kg / mu / time and 5 kg / mu / time respectively. The experiment of not spraying catechin on the leaf surface (i.e., spraying 0 kg / mu on the leaf surface) is used as a blank control; dissolve different masses of catechin in equal mass of water and spray with a drone.

[0058] Implementation method: The rice is transplanted by seedling raising, the transplanting density is 14,000 hills / mu, the fertilizer application rate is 12 kg / mu of nitrogen fertilizer, 4 kg / mu of phosphate fertilizer, and 5 kg / mu of potassium fertilizer. The nitrogen fertilizer is applied according to 50% basal fertilizer + 30% tillering fertilizer + 20% panicle fertilizer, the phosphate fertilizer is applied once as basal fertilizer, and the potassium fertilizer is applied according to 2 / 3 basal fertilizer + 1 / 3 panicle fertilizer. The fertilizer varieties for the test are urea (46% N), superphosphate (12% P 2 O 5 ) and potassium chloride (60% K 2 O). The rice is foliar sprayed with catechin 2 times during the whole growth period, respectively at the early tillering stage of rice (10 days after rice transplanting) and the late tillering stage (40 days after rice transplanting). The spraying time is after 6 pm to reduce the loss of volatilization at high temperature.

[0059] At the late tillering stage of rice, randomly select 5 1 m 2 quadrats for each treatment to investigate the tiller number and plant height of rice. In addition, at the time of rice maturity and harvest, randomly select 5 1 m 2 quadrats for each treatment to measure the nitrogen nutrient content of rice, and the yield is statistically calculated according to the actual production.

[0060] The results show that foliar spraying of catechin with different dosages can increase the plant height, tiller number and yield of rice. In the range of application rate of 1 - 5 kg / mu, it can increase the plant height of rice by 2.0% - 15.4%, the tiller number by 9.1% - 40.9%, the grain yield by 4.4% - 20.6% and the above-ground nitrogen accumulation by 5.8% - 46.6% (Table 3).

[0061] Table 3 Effects of foliar spraying of catechin with different dosages on rice yield

[0062]

[0063] It can be concluded from the above examples that catechin can be used as a biostimulant to effectively promote the growth and development of rice and increase the yield of rice.

[0064] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, rather than all embodiments. People can also obtain other embodiments based on this embodiment without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. Application of catechins in promoting rice growth and development and / or increasing rice yield.

2. The use according to claim 1, characterized in that: The method for promoting the growth and development of rice includes one or more of promoting the growth of the aboveground part of rice, promoting the growth of the underground part of rice, increasing the nitrogen accumulation of the aboveground part of rice, increasing the nitrogen accumulation of the rice root system and increasing the number of rice tillers.

3. The use according to claim 2, characterized in that: The method of promoting the aboveground growth of rice includes increasing one or more of the following: rice plant height, leaf area, total chlorophyll content and aboveground biomass.

4. The use according to claim 2, characterized in that: The promoting the underground growth of rice includes increasing one or more of the total volume, total area and biomass of the rice roots.

5. The use according to claim 1, characterized in that: The catechin application method includes one or more of root irrigation, foliar spraying and broadcasting.

6. The use according to any one of claims 1 to 5, characterized in that: The catechins are administered as biostimulants.

7. A method for promoting rice growth and / or increasing rice yield, characterized in that: include: Catechins are applied to rice.

8. The method according to claim 7, characterized in that The application method comprises root irrigation treatment of rice seedlings; the application concentration of the catechins is 5-50 mg / L.

9. The method according to claim 7, characterized in that: The application method includes broadcasting; the application amount of the catechin is 0.5-4 kg / mu; the broadcasting includes broadcasting the catechin with basal fertilizer.

10. The method according to claim 7, characterized in that The application method includes foliar spraying; the application amount of the catechin is 1-5 kg / mu / time, and it is applied twice in total; the two application periods include the early tillering stage and the late tillering stage of rice.

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