Composition for improving conversion efficiency of chicken feed and application thereof

Abstract

The invention discloses a composition for improving the conversion efficiency of chicken feed and application of the composition. The composition comprises Brucella Romummii metabiotics, farnesol and paeonol in a mass ratio of 2: 1: 1. The composition is directly added into a feed, and the addition amount of the composition is 1% of the total weight of the chicken feed. The invention finds that when the composition formed by mixing the Brucella Romummiana metagen, the farnesol and the paeonol is used as a feed additive, the safety is high, the toxic and side effects are avoided, the conversion efficiency of broiler feed can be obviously improved, the growth performance and the meat quality of broilers are improved, and the egg laying performance and the egg quality of laying hens are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of healthy and efficient chicken breeding, and particularly relates to a composition for improving the feed conversion efficiency of chickens and its application. Background Art

[0002] In China, the output of poultry meat / eggs ranks among the top in the world. Among the meat consumption in China, poultry meat accounts for the second place after pork, and eggs are the protein sources with a high proportion in the daily consumption of residents. Therefore, ensuring the healthy and efficient breeding of chickens and strictly controlling the output and quality of poultry meat / eggs are of great significance to the national economy and people's livelihood. However, due to the stimulation of various stressors during the breeding process, such as abnormal temperature changes, pathogen infections, transportation stress, excessive vaccination, and harmful factors in feed sources, the production performance and meat / egg quality of chickens are negatively affected; this severely restricts the economic benefits of farmers or chicken production enterprises.

[0003] Therefore, developing green, safe and efficient feed additive products to improve the production performance and meat / egg quality of chickens is one of the key research topics in the livestock industry. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide a composition for improving the feed conversion efficiency of chickens and its application. The composition, by mass, includes 2 parts of Rombutsia postbiotics, 1 part of farnesol, and 1 part of paeonol. The composition has high safety, stable efficacy, no toxic and side effects, can significantly improve the feed conversion efficiency of chickens, and improve meat / egg quality. The composition can be used to prepare a chicken feed additive for improving the feed conversion efficiency of chickens.

[0005] To achieve the above object, the present invention provides at least the following technical solutions:

[0006] In the first aspect, the present invention provides a composition for improving the feed conversion efficiency of chickens, and the composition includes Rombutsia postbiotics, farnesol, and paeonol.

[0007] In the second aspect, the present invention provides the application of the foregoing composition in preparing a chicken feed additive.

[0008] The composition for improving the feed conversion efficiency of chickens and its application provided by the present invention, compared with the prior art, has at least the following advantages:

[0009] 1. Postbiotics of Romboutsia can overcome the disadvantages such as difficult colonization of live bacteria, easy degradation by gastric acid and bile salts, and then target the intestine to play a role in improving intestinal health; farnesol and paeonol are derived from natural plants, with high safety and no toxic side effects. The composition formed by mixing postbiotics of Romboutsia, farnesol and paeonol can be used as a feed additive for a long time to achieve the purpose of improving feed conversion efficiency; long-term use will not lead to the generation of drug-resistant strains, which is an advantage that other anti-disease drugs do not have.

[0010] 2. In vivo experiments show that the composition formed by mixing postbiotics of Romboutsia, farnesol and paeonol can improve the feed conversion efficiency of broilers and the meat quality; improve the laying performance and egg quality of laying hens. Detailed implementation manners

[0011] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0012] Those skilled in the art of the present technology can understand that unless specifically stated, the "said", "this", "aforementioned" used in the text of this application may also include plural forms. It should be further understood that the term "including" used in the description of this application means that there are the described features, steps, operations, but does not exclude the existence or addition of one or more other features, integers, steps.

[0013] Those skilled in the art of the present technology can understand that for those not specifying specific experimental steps or conditions in the embodiments, the operations or conditions of the conventional experimental steps described in the literature in this field can be followed; for those raw materials or instrument devices not specifying the manufacturer, they are all conventional products that can be obtained through commercial purchase.

[0014] Those skilled in the art of the present technology can understand that unless otherwise specified in this application, when the embodiments give a numerical range, any value at both ends of each numerical range and any value between the two ends can be selected. Unless otherwise defined, all technical and scientific terms used in this application, based on the understanding of those skilled in the art of the present technology of the prior art and the description of this application, can also use any method, device and material of the prior art similar or equivalent to the methods, devices and materials described in the embodiments of this application to implement this application.

[0015] Romboutsia (RB) is mainly colonized in the intestinal lumen and mucosa of the host ileum, and its metabolism can produce various bioactive substances such as β-glucan, functional amino acids, vitamins, propionic acid and butyric acid. In recent years, due to its high correlation with the health of the body, RB has been widely concerned in the field of life medicine. For example, studies have found that the relative abundance of RB in the intestinal lumen of patients with autism, hypertension and diabetes is significantly down-regulated. In the intestine of adult broilers, the abundance of RB is second only to lactic acid bacteria, and it has great potential in regulating the intestinal health of broilers and improving their growth performance. RB postbiotics prepared based on RB are rich in β-glucan, functional amino acids, vitamins, propionic acid and butyric acid, and also include a high content of bacterial protein. Compared with pure live bacteria, RB postbiotics can overcome the disadvantages of difficult live bacteria colonization, easy degradation by gastric acid and bile salts, target the intestine and then play a role in improving intestinal health. However, there is no report on RB postbiotics in poultry production at present.

[0016] Farnesol is an active ingredient extracted from the bark of Acacia farnesiana, and its main components are flavonoids and triterpenoids. Farnesol can also be found in citronella, lemon grass, tuberose, cyclamen, rose, neroli, balsam and musk. Its chemical formula is C 15 H 25 OH, with a molecular weight of 223.72 g / mol. Studies have found that farnesol has various biological functions such as antibacterial, anti-inflammatory, antioxidant, immune regulation and lipid metabolism. Clinically, farnesol is used to treat allergic asthma, diabetes, atherosclerosis, obesity and hyperlipidemia, etc. Due to its green and safe characteristics, farnesol is widely used in food, skin care products, medicine and other fields. In livestock production, there are few reports on farnesol.

[0017] Paeonol is a traditional Chinese medicine extracted from the root bark of Paeonia suffruticosa Andr., and can also be extracted from the dried root bark of Paeonia lactiflora Pall., a plant of the Ranunculaceae family. Paeonol is a white or slightly yellowish needle-shaped crystal, with a melting point of about 49-51 °C, easily soluble in ethanol and methanol, and slightly soluble in water. Paeonol can be distilled with water vapor and has a strong absorption in the ultraviolet region; the energy of paeonol at a wavelength of 274 nm is 862, so it can be measured by a spectrophotometer. Current studies have found that paeonol has analgesic, anti-inflammatory, antipyretic and anti-allergic effects. Specifically, paeonol can relieve pain responses caused by the stimulation of substances such as tail pressure and acetic acid; it can relieve inflammatory responses caused by egg white, formaldehyde, histamine, 5-hydroxytryptamine, bradykinin and endotoxin, etc.; it can relieve the body fever caused by vaccine immunization. In addition, paeonol also has various biological functions such as antibacterial, anti-inflammatory, antioxidant, blood pressure and lipid regulation. Due to its green, safe and efficient characteristics, paeonol is used as a clinical drug in human medicine, such as paeonol ointment, but there is no report on paeonol in livestock production.

[0018] At present, there are few reports on the application of Rombutsia postbiotics, farnesol, and paeonol in chicken production, and there is no report on their application as a composition in chicken production. On this basis, in the embodiments of the present invention, Rombutsia postbiotics, farnesol, and paeonol are mixed in a certain proportion to form a composition. In vivo experiments have shown that this composition can improve the feed conversion efficiency of broilers and improve meat quality; it can also improve the laying performance and egg quality of laying hens, providing basic data for the efficient breeding of chickens.

[0019] Based on this, an embodiment of the present invention provides a composition for improving the feed conversion efficiency of chickens, and the composition includes Rombutsia postbiotics, farnesol, and paeonol.

[0020] In some preferred embodiments, the composition, by mass, includes 2 parts of Rombutsia postbiotics, 1 part of farnesol, and 1 part of paeonol.

[0021] In some embodiments, the strain of Rombutsia used to prepare Rombutsia postbiotics is the commercially available Romboutsia ilealis DSM 25109.

[0022] In some embodiments, the cell protein in the Rombutsia postbiotics is ≥ 33%, the Rombutsia is ≥ 10 8 cfu / g, the β-glucan content is ≥ 5 mg / g, and the sodium butyrate content is ≥ 10 mg / g.

[0023] In some preferred embodiments, the preparation method of the Rombutsia postbiotics is as follows:

[0024] Inoculate a single colony of Romboutsia ilealis DSM 25109 into 10 mL of modified GAM liquid medium and anaerobically culture it at 37°C for 24 hours; then take 1 mL of the bacterial liquid and inoculate it into 100 mL of modified GAM liquid medium; then take 100 mL of the bacterial liquid and place it in a 500 L fermenter for expansion culture for 24 hours, keeping the temperature of the fermenter constant at 37°C and filling it with N 2 Establish anaerobic fermentation conditions; after the culture is completed, add 10% of maltodextrin to the fermentation broth for adsorption, and spray-dry to prepare Rombutsia postbiotics.

[0025] Based on this, an embodiment of the present invention provides the application of the foregoing composition in the preparation of chicken feed additives.

[0026] In some embodiments, the usage method of the feed additive is: directly add this composition to the feed, and the addition amount is 1% of the total weight of the chicken feed.

[0027] The technical solutions and achieved technical effects of the present invention will be described in detail through more specific embodiments below.

[0028] Example 1

[0029] This embodiment provides a composition for improving chicken feed conversion efficiency, which is prepared by the following method:

[0030] Weigh 2000 g of Rombutsiella postbiotics (bacterial protein ≧33%, Rombutsiella ≧108 cfu / g, β-glucan content ≧5 mg / g, sodium butyrate content ≧10 mg / g), 1000 g of farnesol (purchased from Aladdin, with a purity greater than 80%), and 1000 g of paeonol (purchased from Aladdin, with a purity greater than 85%), and mix them evenly to obtain the composition for improving chicken feed conversion efficiency.

[0031] The composition can be used in normal broiler or laying hen feeding, and is added to feed at an amount of 1% of the total weight of feed, or added to drinking water at 1% of the weight of drinking water, and is used throughout the entire feeding cycle.

[0032] Example 2

[0033] This example verifies the effect of the composition prepared in Example 1 on the feed conversion efficiency and meat quality of broiler chickens. The verification method is as follows:

[0034] 1. Materials and Methods

[0035] The experiment involved 240 healthy AA + Broiler male chicks were divided into two treatment groups, each treatment group included 8 replicate groups, and each replicate group had 15 chickens; the two treatment groups were: a control group and a combination group; the broilers in the control group were fed a "corn-soybean meal" type basal diet, and the diet formula was formulated with reference to the nutritional requirements standard of Chinese broilers (NY / T33-2004); the broilers in the combination group were fed a diet in which the composition prepared in Example 1 was added to the basal diet at an amount of 1% of the total weight of the feed; the feeding experiment lasted for 35 days, during which Broilers were allowed to eat and drink freely and adopted a 24-hour lighting system. On the 35th day of the experiment, all broilers were fasted for 8 hours, then weighed and feed consumption was counted, and growth performance-related indicators such as average daily gain (ADG), average daily feed intake (ADFI), feed-to-weight ratio (FCR) were calculated. Two broilers with uniform weight were selected from each replicate group and anesthetized by intravenous injection of sodium pentobarbital (at a dose of 50 mg / kg live weight) under the wing, and then slaughtered. The breast and leg muscles were dissected for subsequent analysis.

[0036] 2. Data Analysis

[0037] The experimental data were analyzed by independent-sample T-test using One-way ANOVA in SPSS 23.0 statistical software. The significance level for difference was set at P < 0.05, and the highly significant level for difference was set at P < 0.01. The results were expressed as mean ± standard deviation.

[0038] 3. Experimental Results

[0039] 3.1 Effects of the Composition on the Growth Performance of Broiler Chickens

[0040] Table 1 shows the results of the effects of the composition on the growth performance of broiler chickens. Different superscript letters in the table indicate significant differences (P < 0.05). The survival rate was calculated as the number of broiler chickens at 35 days of age divided by the number at the start of 1 day of age.

[0041] Table 1

[0042] Test items Control group Composition group P-value d35 - Average body weight, g <![CDATA[2017.63±52.18 b > <![CDATA[2209.12±82.34 a > 0.019 ADFI, g 86.73±4.15 90.26±3.05 0.062 ADG, g <![CDATA[59.32±1.99 b > <![CDATA[64.91±2.73 a > 0.031 FCR <![CDATA[1.47±0.03 a > <![CDATA[1.39±0.02 b > <0.001 Survival rate, % 94.33±1.98 96.14±2.05 0.052

[0043] As can be seen from Table 1, adding the composition formed by mixing postbiotics of Romboutsia, farnesol and paeonol to the diet significantly increased the average weight of broiler chickens at 35 days of age and the average daily gain during the experimental period, decreased the feed-to-weight ratio, and there was a tendency to increase the survival rate of broiler chickens (P = 0.052). The experimental results found that the composition provided by the present invention improved the feed conversion efficiency of broiler chickens and increased the survival rate of broiler chickens by about 1.81%, which has important application value for farmers or enterprises.

[0044] 3.2 Effects of the Composition on the Meat Quality of Broiler Chickens

[0045] Table 2 shows the results of the effects of the composition on the meat quality of broiler chickens. Different superscript letters in the table indicate significant differences (P < 0.05). The muscle ratio was expressed as the ratio of the muscle weight to the body weight of the corresponding broiler chicken × 100%. The pH value was measured using a pH meter, and the shear force was detected using a computer-controlled muscle tenderness tester (C-LM4, Beijing Tianxiang Feiyu Technology Co., Ltd.). The specific steps were briefly described as follows: A 2 cm × 2 cm × 2 cm meat sample was peeled from the whole chicken breast, placed in a sealed plastic bag, and put into an 80 °C constant temperature water bath. After heating for 9.5 min with the lid on, it was taken out and cooled at room temperature for 30 min. Then the liquid in the bag was drained, and the meat sample was taken out and weighed. The ratio of the weight loss of the muscle sample before the test minus the weight at this time to the muscle weight before the test × 100% represented the cooking loss rate of the muscle. Further, the square meat was placed horizontally on the blade position of the tenderness tester along the muscle fiber direction for shearing operation. Each piece of meat was cut 2 times, and the average value of the 2 shear forces was calculated, and its unit was Newton (N).

[0046] Table 2

[0047]

[0048] As can be seen from Table 2, adding the composition formed by mixing Rombutz postbiotics, farnesol and paeonol to the diet significantly increased the thigh muscle rate of broilers, had a tendency to decrease the pH value of breast muscle (P = 0.063), significantly reduced the cooking loss of broiler breast and thigh muscles, reduced the shear force of thigh muscles, and had a tendency to decrease the shear force of breast muscles (P = 0.074). The lower the pH value, the more acidic the muscle, which will affect palatability; the greater the cooking loss, the less meat consumers can actually utilize; the lower the shear force value, the higher the tenderness of chicken. The results in Table 2 show that the composition provided in the examples of the present invention improves the deposition efficiency of muscle and increases the tenderness of muscle, thereby improving meat quality.

[0049] Example 3

[0050] In this example, the effects of the composition prepared in Example 1 on the feed conversion efficiency and blood biochemical indexes of broilers under the low-energy model were verified, and the verification method is as follows:

[0051] 1. Materials and Methods

[0052] In the experiment, 360 one-day-old, uniformly weighted and healthy "Cobb 500" male broiler chicks were evenly divided into 3 treatment groups. Each treatment group included 8 replicate groups, and each replicate group included 15 chickens; the 3 treatment groups were: control group, low-energy group, low-energy + composition group; the broilers in the control group were fed a "corn-soybean meal" type basal diet, and the diet formula was formulated with reference to the nutritional requirements standard for Chinese broilers (NY / T33-2004); the diet fed to the broilers in the low-energy group was to reduce the energy by 60 kcal / ton of feed by reducing soybean oil on the basis of the basal diet level, and the diet fed to the broilers in the low-energy + composition group was to add the composition prepared in Example 1 at an addition amount of 1% of the total feed weight on the diet level of the broilers in the low-energy group; the feeding experiment lasted for 35 days. During this period, the broilers were allowed to freely eat and drink, and a 24-hour lighting system was adopted; on the 35th day of the experiment, all broilers were fasted for 8 hours, then weighed and the feed consumption was counted, and growth performance-related indexes such as average daily gain (ADG), average daily feed intake (ADFI), and feed-to-gain ratio (FCR) were calculated. And 2 broilers with uniform body weight were selected from each replicate group to collect wing vein blood for the determination of blood biochemical indexes.

[0053] 2. Data Analysis

[0054] The experimental data were analyzed by one-way ANOVA in the SPSS 23.0 statistical software, and the Duncan method was used for multiple comparisons between groups. P < 0.05 was used as the standard for significant differences, and P < 0.01 was used as the standard for extremely significant differences; the results were expressed as mean ± standard deviation.

[0055] 3. Experimental Results

[0056] 3.1 Effects of the composition on the growth performance of broilers under the low - energy model

[0057] Table 3 shows the results of the effects of the composition on the growth performance of broilers under the low - energy model. Different superscript letters in the table indicate significant differences (P < 0.05).

[0058] Table 3

[0059]

[0060]

[0061] When the energy of poultry diet decreases, its feed conversion efficiency becomes worse. As can be seen from Table 3, under the low - energy model, the feed conversion efficiency of broilers decreases (manifested as an increase in the feed - to - gain ratio), and the average daily gain of broilers also decreases. However, adding the composition prepared by the present invention under the low - energy model can significantly improve the average daily gain and the feed conversion efficiency of broilers.

[0062] 3.2 Effects of the composition on the blood biochemical indexes of broilers under the low - energy model

[0063] Table 4 shows the results of the effects of the composition on the blood biochemical indexes of broilers under the low - energy model. Different superscript letters in the table indicate significant differences (P < 0.05); the blood biochemical indexes were measured using a Hitachi automatic blood biochemical analyzer, where TP represents total protein, ALB represents albumin, TB represents total bilirubin, DB represents direct bilirubin, AST represents aspartate aminotransferase, ALT represents alanine aminotransferase, ALP represents alkaline phosphatase, GGT represents γ - glutamyl transpeptidase, TG represents triglyceride, TC represents total cholesterol, HDL represents high - density lipoprotein, LDL represents low - density lipoprotein, CREA represents creatinine, UA represents uric acid, GLU represents glucose, and LDH represents lactate dehydrogenase.

[0064] Table 4

[0065] Test items Control group Low-energy group Low-energy + composition group P value TP (g / L) 33.67±3.17 35.64±4.00 35.35±2.47 0.379 ALB (g / L) 15.25±0.98 15.74±1.18 15.50±0.87 0.730 TB (g / L) <![CDATA[14.16±2.20 b > <![CDATA[17.87±2.61 a > <![CDATA[14.21±1.07 b > 0.016 DB (μmol / L) <![CDATA[1.29±0.36 b > <![CDATA[1.79±0.42 a > <![CDATA[1.14±0.49 b > <0.001 ALT (U / L) 1.29±0.36 1.79±0.42 1.84±0.49 0.941 AST (U / L) 240.91±34.45 296.75±54.50 251.45±72.78 0.061 ALP (U / L) <![CDATA[2086.68±522.27 b > <![CDATA[2940.05±711.54 a > <![CDATA[1458.26±325.99 c > <0.001 GGT (U / L) 16.50±3.78 18.08±3.92 17.58±3.23 0.791 TG (mmol / L) 0.36±0.07 0.35±0.05 0.37±0.03 0.744 TC (mmol / L) 3.37±0.32 3.54±0.43 3.47±0.32 0.555 HDL (mmol / L) 2.25±0.28 2.41±0.30 2.32±0.22 0.548 LDL (mmol / L) 0.51±0.14 0.51±0.15 0.54±0.12 0.819 CREA (μmol / L) 8.95±2.38 8.87±2.63 8.71±1.72 0.881 UA (μmol / L) 225.20±63.32 255.24±64.49 258.70±62.39 0.254 GLU (mmol / L) 13.44±1.35 13.10±0.59 13.58±1.30 0.733 LDH (U / L) 945.89±728.46 930.61±263.82 976.49±279.91 0.570

[0066] As can be seen from Table 4, under the low - energy model, the total bilirubin, direct bilirubin, alkaline phosphatase, and aspartate aminotransferase in the serum of broilers increase, indicating that low energy has a negative impact on the liver of broilers. Under the low - energy model conditions, adding the composition prepared by the present invention to the diet can alleviate the increase in the contents of total bilirubin, direct bilirubin, alkaline phosphatase, and aspartate aminotransferase, indicating that the composition has a protective effect on the liver of broilers. The liver is the main site of nutrient metabolism and an important immune organ of the body. This protective effect of the composition on the liver of broilers is also one of the reasons for its improvement of the feed conversion efficiency of broilers.

[0067] Example 4

[0068] In this example, the effects of the composition on the laying performance and egg quality of laying hens were verified, and the verification method is as follows:

[0069] 1. Materials and Methods

[0070] In the experiment, 480 healthy Jingfen No. 1 laying hens at 21 weeks of age with uniform body weight were randomly divided into two treatment groups: a control group and a composition group. Each treatment group had 8 replicate groups, and each replicate group had 30 laying hens. The laying hens in the control group were fed a "corn-soybean meal" based basal diet, and the diet formula was formulated according to the nutritional requirement standard for Chinese laying hens (NY / T 33-2004). The laying hens in the composition group were fed a diet with the composition prepared in Example 1 added at a rate of 1% of the total feed weight on the basis of the basal diet. The experiment lasted for 5 weeks. During the experiment, the laying hens had free access to food and water, and a lighting regime of 18 hours of light and 6 hours of darkness was adopted. During the experiment, the egg weight was counted every day, and the laying rate and feed consumption were counted every week, and the feed-to-egg ratio was calculated. At the end of the experiment, 60 eggs were randomly selected from each treatment group for egg quality analysis.

[0071] 2. Data Analysis

[0072] The experimental data were analyzed by independent sample T-test using One-way ANOVA in SPSS 23.0 statistical software. The significance level for difference was set at P < 0.05, and the extremely significant level for difference was set at P < 0.01. The results were expressed as mean ± standard deviation.

[0073] 3. Experimental Results

[0074] 3.1 Effects of the Composition on the Laying Rate of Laying Hens

[0075] Table 5 shows the results of the effects of the composition on the laying rate of laying hens. Different superscript letters in the table indicate significant differences at P < 0.05.

[0076] Table 5

[0077] Time Control group Composition group P value Week 1 25.61±2.43 26.12±3.32 0.484 Week 2 51.24±3.65 52.11±3.37 0.628 Week 3 <![CDATA[74.08±5.11 b > <![CDATA[81.42±3.22 a > 0.016 Week 4 <![CDATA[84.32±3.21 b > <![CDATA[90.39±5.15 a > 0.021 Week 5 91.90±2.91 93.47±4.43 0.056

[0078] As can be seen from Table 5, adding the composition provided by the present invention to the diet can significantly improve the laying rate of laying hens in the 3rd, 4th, and 5th weeks of the experiment.

[0079] 3.2 Effects of the Composition on the Feed-to-Egg Ratio of Laying Hens

[0080] Table 6 shows the results of the effects of the composition on the feed-to-egg ratio of laying hens. The results are expressed as the feed-to-egg ratio of laying hens in each week of the experiment and during the whole experiment. The feed-to-egg ratio represents the weight of feed (jin) consumed to produce 1 jin of eggs.

[0081] Table 6

[0082]

[0083]

[0084] As can be seen from Table 6, the composition formed by mixing postbiotics of Romboutsia, farnesol and paeonol in the diet can improve the feed-to-egg ratio of laying hens in the 3rd, 4th and 5th weeks of the experiment, and has an improving effect on the feed-to-egg ratio during the whole experiment period. The experimental results show that the composition provided by the present invention can improve the feed conversion efficiency of laying hens and save the breeding cost.

[0085] 3.3 Effects of the composition on egg quality

[0086] Table 7 shows the results of the effects of the composition on egg quality. The egg quality detection method is as follows:

[0087] After weighing the egg weight with an analytical balance, the eggshell strength (kg / cm 2 ) and egg yolk color were measured with an egg quality detector DET-6000 (NABEL Co., Ltd, Japan); specifically, the egg was placed vertically on the eggshell strength detector with the blunt end upward, and the pressure borne per unit area of the eggshell surface was measured; the albumen height (mm) was measured with an albumen height detector (KIYA-818B, SEISAKUSHO, LTD), and the Haugh unit was calculated according to the formula; Haugh unit = 100·lg(H - 1.7W 0.37 + 7.57), where H is the albumen height (mm) and W is the egg weight (g); the eggshell thickness was measured with a micrometer (after removing the shell membrane, the thicknesses of the middle, blunt and sharp ends of the egg were measured and averaged, in millimeters, accurate to 0.01 mm).

[0088] Table 7

[0089] Test items Control group Composition group P value Albumen height, mm 6.33±0.95 6.56±0.87 0.845 Haugh unit 73.17±8.22 75.35±5.09 0.511 Yolk color 6.38±0.77 6.41±0.78 0.798 <![CDATA[Eggshell strength, kg / cm 2 > 40.15±3.15 44.01±2.18 0.029 Eggshell thickness, cm 0.33±0.01 0.36±0.01 0.002 Yolk ratio, % 22.33±1.52 23.06±1.67 0.176 Albumen ratio, % 66.87±1.44 65.77±0.89 0.221 Eggshell ratio, % 10.75±0.38 10.51±0.99 0.537

[0090] As can be seen from Table 7, the composition formed by mixing postbiotics of Romboutsia, farnesol and paeonol in the diet can increase the eggshell strength and eggshell thickness of eggs. The higher the eggshell strength and thickness of eggs, the stronger their characteristics of being resistant to storage and transportation. The above results show that the composition provided by the present invention improves the egg quality by improving the eggshell quality of eggs.

[0091] In summary, the composition formed by mixing postbiotics of Romboutsia, farnesol and paeonol provided by the present invention has the ability to improve the conversion efficiency of chicken feed, and this composition can be used to prepare chicken feed additives.

[0092] The present invention has been described in detail above. Specific examples are used herein to illustrate the principle and implementation of the present invention. The description of the above embodiments is only for helping to understand the present invention and its core idea. It should be noted that for those of ordinary skill in the art in this technical field, without departing from the principle of the present invention, several improvements and modifications can be made to the present invention, and these improvements and modifications also fall within the protection scope of the claims of the present invention.

Claims

1. A composition for improving chicken feed conversion efficiency, the composition comprising Rombutsiella postbiotics, farnesol and paeonol.

2. The composition according to claim 1, characterized in that Calculated by mass, it includes 2 parts of Rombutsiella postbiotics, 1 part of farnesol and 1 part of paeonol.

3. The composition according to claim 1, characterized in that The strain of Romboutsia ilealis used for preparing the Romboutsia postbiotics is commercially available Romboutsia ilealis DSM 25109.

4. The composition according to claim 1, characterized in that The bacterial protein in the Rombutsiella postbiotics is ≥33%, and the Rombutsiella is ≥10 8 cfu / g, β-glucan content ≥5mg / g, sodium butyrate content ≥10mg / g.

5. The composition according to claim 1, characterized in that The preparation method of the Rombutsiella postbiotics is as follows: A single colony of Romboutsia ilealis DSM 25109 was inoculated into 10 mL of a modified GAM liquid culture medium and cultured anaerobically at 37° C. for 24 hours; thereafter, 1 mL of the bacterial solution was inoculated into 100 mL of the modified GAM liquid culture medium; thereafter, 100 mL of the bacterial solution was placed in a 500 L fermenter for expansion culture for 24 hours, the temperature of the fermenter was kept constant at 37° C., and N2 was filled to establish anaerobic fermentation conditions; after the culture was completed, 10% maltodextrin was added to the fermentation liquid for adsorption, and spray drying was performed to prepare Romboutsia postbiotics.

6. Use of the composition according to any one of claims 1 to 5 in the preparation of a chicken feed additive.

7. The use according to claim 6, characterized in that: The method for using the feed additive is: directly adding the composition to feed, and the added amount is 1% of the total weight of the chicken feed.

Images