An enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation and its preparation method

Abstract

The present invention discloses an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation and its preparation method, belonging to the field of biomedicine. An enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation includes traditional Chinese medicine components, an enzyme and a carrier, wherein the enzyme is adapted to the traditional Chinese medicine components; the carrier includes an adsorbent and a blocking layer wrapping the adsorbent; wherein, the adsorbent is a modified porous yeast cell wall; the modified porous yeast cell wall is a modified product obtained by enzymatically hydrolyzing and expanding the pores of the yeast cell wall with β-1,3-glucanase and then performing surface modification with carboxymethyl chitosan. The enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation of the present invention enriches the enzymatically hydrolyzed products of traditional Chinese medicine with the adsorbent, and then wraps the adsorbent with the blocking layer to prevent the leakage of the enzymatically hydrolyzed products of traditional Chinese medicine, obtaining a dry powder preparation that can be stored for a long time.

Description

Technical Field

[0001] The present invention belongs to the field of biomedicine and relates to an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation and a preparation method thereof. Background Art

[0002] Traditional Chinese medicine components are the main components of traditional Chinese medicine health-preserving decoctions. At present, the traditional Chinese medicine component preparations for traditional Chinese medicine health-preserving decoctions mainly adopt two forms: one is a prefabricated liquid decoction, which is convenient for processing and has a good taste, but has a short shelf life (usually requires refrigeration and does not exceed 7 days), and has a high transportation cost; the other is to directly use traditional Chinese medicinal materials. The medicinal materials have a large volume, a long boiling time (usually 2 - 3 hours, which is not conducive to obtaining effective components), and the medicinal residues affect the taste (such as bitter taste or rough texture). There is a lack of a traditional Chinese medicine component preparation that is both convenient for storage and convenient for obtaining the effective components of traditional Chinese medicine. Summary of the Invention

[0003] The purpose of the present invention is to provide an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation and a preparation method thereof, which solve the problem that there is currently a lack of a traditional Chinese medicine component preparation for traditional Chinese medicine health-preserving decoctions that is both convenient for storage and convenient for obtaining the effective components of traditional Chinese medicine.

[0004] The technical solution adopted by the present invention is as follows:

[0005] An enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation, comprising traditional Chinese medicine components, an enzyme, and a carrier, wherein the enzyme is adapted to the traditional Chinese medicine components;

[0006] The carrier includes an adsorbent and a blocking layer that wraps the adsorbent; wherein, the adsorbent is a modified porous yeast cell wall;

[0007] The modified porous yeast cell wall is a modified product obtained by enzymatically expanding the pores of the yeast cell wall with β-1,3-glucanase and then performing surface modification with carboxymethyl chitosan.

[0008] In the present invention, enzymes are used to enzymatically hydrolyze traditional Chinese medicine components to obtain enzymatically hydrolyzed traditional Chinese medicine products. The present invention utilizes the directional enzyme β-1,3-glucanase to expand the pores of yeast cell walls, increase their porosity, and form a porous structure. The β-1,3-glucanase only hydrolyzes the β-1,3 bonds of the yeast wall to form pores with a size of 2-5 nm, which allows small molecule substances to enter and blocks hydrolytic enzymes for macromolecular substances such as traditional Chinese medicine components, avoiding the occupation competition of enzymes and other macromolecular substances with small molecule traditional Chinese medicine enzymatically hydrolyzed products on the yeast cell wall, and providing sufficient storage space for traditional Chinese medicine enzymatically hydrolyzed products. The pore expansion in this application does not refer to enlarging the pore diameter, but rather increasing the proportion of the number of pores. After the directional enzyme pore expansion, the present application further modifies the pore-expanded yeast cell wall with carboxymethyl chitosan. Without affecting the release of enzymatically hydrolyzed products after reconstitution of the dry powder, carboxymethyl chitosan enhances the adsorption strength of the yeast cell wall for substances entering its internal pores. The present invention realizes the targeted adsorption of small molecule enzymatically hydrolyzed products by means of pore size screening and adsorption enhancement, which is beneficial to increasing the content of small molecule enzymatically hydrolyzed products in the final dry powder preparation and reducing the adsorption effect of enzymes and other major macromolecular substances on small molecule enzymatically hydrolyzed products.

[0009] After the modified porous yeast cell wall adsorbs traditional Chinese medicine enzymatically hydrolyzed products in the present invention, in order to prevent the leakage of traditional Chinese medicine enzymatically hydrolyzed products from the modified porous yeast cell wall, a blocking layer is used to wrap the modified porous yeast cell wall adsorbed with traditional Chinese medicine enzymatically hydrolyzed products, which is also beneficial for the long-term preservation of traditional Chinese medicine enzymatically hydrolyzed products.

[0010] Further, the modified porous yeast cell wall is prepared by the following method:

[0011] A1. At 50 °C, use 10 U / g of β-1,3-glucanase to enzymatically hydrolyze the yeast cell wall for 1.5 - 2 hours to obtain a porous yeast cell wall with 2 - 5 nm micropores distributed thereon;

[0012] A2. Dissolve carboxymethyl chitosan in MES buffer to obtain a carboxymethyl chitosan solution, add 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide to the carboxymethyl chitosan solution, and stir and react to obtain an activated carboxymethyl chitosan solution;

[0013] A3. Disperse the porous yeast cell wall in MES buffer to obtain a porous yeast cell wall suspension;

[0014] A4. Add the activated carboxymethyl chitosan solution to the porous yeast cell wall suspension, stir and react for 4 - 5 hours, then add glycine, stir for 30 - 40 minutes and then separate to obtain a precipitate, which is then washed and freeze-dried to obtain the modified porous yeast cell wall.

[0015] Further, the mass ratio of the porous yeast cell wall to carboxymethyl chitosan is 9 - 10:1.

[0016] Furthermore, the addition amount of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride is 2% of the mass of carboxymethyl chitosan, and the addition amount of N-hydroxysuccinimide is 1% of the mass of carboxymethyl chitosan.

[0017] Furthermore, the blocking layer comprises the following components: sodium alginate, gelatin, resistant dextrin and a cross-linking agent.

[0018] Furthermore, the traditional Chinese medicine components comprise the following components: ginseng, polygonatum sibiricum, cordyceps militaris, astragalus membranaceus, and the mass ratio of ginseng, polygonatum sibiricum, cordyceps militaris, astragalus membranaceus is 4:2:2:1.

[0019] The present invention gives a specific traditional Chinese medicine component in combination with the actual research and development situation.

[0020] Furthermore, the enzyme is a composite enzyme, and the mass ratio of the composite enzyme to the traditional Chinese medicine component is 1:50-52. The composite enzyme comprises the following components: cellulase, protease, amylase, β-glucosidase, the pH of enzymatic hydrolysis is 5-6, and the mass ratio of cellulase, protease, amylase, β-glucosidase is 1:0.5:0.5:1.

[0021] The enzyme in the present invention is selected based on the traditional Chinese medicine component.

[0022] Furthermore, the mass ratio of resistant dextrin, sodium alginate to gelatin is (3-4):(1-2):1.

[0023] A preparation method of an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation comprises the following steps:

[0024] S1. Obtain a traditional Chinese medicine composition, including ginseng, polygonatum sibiricum, cordyceps militaris, astragalus membranaceus, boil the traditional Chinese medicine composition in water for 1-1.5 h, and filter to obtain a primary traditional Chinese medicine extract;

[0025] S2. Use beer yeast cell wall as a raw material, perform enzymatic treatment with β-1,3-glucanase to expand pores, and then perform surface modification with carboxymethyl chitosan, filter and dry to obtain a modified porous yeast cell wall;

[0026] S3. Cool the primary traditional Chinese medicine extract to 50 °C, adjust the pH to 5-6, then add cellulase, protease, amylase, β-glucosidase, perform enzymatic hydrolysis at 50 °C for 2 hours, add the modified porous yeast cell wall, continue to react for 1 hour, and filter to obtain a modified porous yeast cell wall loaded with enzymatically hydrolyzed traditional Chinese medicine components;

[0027] S4. Add resistant dextrin to water, stir until completely dissolved, then add sodium alginate and gelatin, and stir at a constant temperature of 50 °C for 1 h to obtain a liquid mixture for the encapsulation layer. Then disperse the modified porous yeast cell wall loaded with the enzymatically hydrolyzed components of traditional Chinese medicine in the liquid mixture for the encapsulation layer, stir evenly, and then dropwise add a CaCl2 cross-linking agent for cross-linking reaction. After the reaction, filter and spray-dry to obtain the dry powder of the modified porous yeast cell wall loaded with the enzymatically hydrolyzed components of traditional Chinese medicine encapsulated by the encapsulation layer, which is the dry powder preparation of the enzymatically hydrolyzed traditional Chinese medicine decoction.

[0028] Furthermore, the modified porous yeast cell wall and the crude extract of traditional Chinese medicine are mixed in a ratio of 15 - 20% w / v.

[0029] In summary, due to the adoption of the above technical solutions, the beneficial effects of the present invention are as follows:

[0030] 1. The dry powder preparation of the enzymatically hydrolyzed traditional Chinese medicine decoction of the present invention enriches the enzymatically hydrolyzed products of traditional Chinese medicine with an adsorbent, and then encapsulates the adsorbent with an encapsulation layer to prevent the leakage of the enzymatically hydrolyzed products of traditional Chinese medicine, thus obtaining a dry powder preparation that can be stored for a long time.

[0031] 2. In the present invention, the adsorbent is a modified porous yeast cell wall. The porous yeast cell wall modified with carboxymethyl chitosan has a targeted and efficient adsorption effect on the enzymatically hydrolyzed products of traditional Chinese medicine. Moreover, after the surface modification with carboxymethyl chitosan, it can not only significantly improve the thermal stability of the porous yeast cell wall but also block the influence of the enzymes for enzymatically hydrolyzing traditional Chinese medicine on the yeast cell wall, enabling the modified porous yeast cell wall to stably exist in the weakly acidic environment of enzymatically hydrolyzing traditional Chinese medicine without hydrolysis. However, when the dry powder preparation of the enzymatically hydrolyzed traditional Chinese medicine decoction of the present application is added to the health-preserving decoction and subjected to high-pressure boiling, at a temperature above 100 °C, along with the disintegration of the encapsulation layer, the modified porous yeast cell wall will rupture and release the adsorbed enzymatically hydrolyzed products of traditional Chinese medicine. The enzymatically hydrolyzed products of traditional Chinese medicine after enzymatic hydrolysis enter the traditional Chinese medicine health-preserving decoction to play the corresponding health-preserving role, and the ruptured modified porous yeast cell wall has a small volume and does not need to be removed, which is also beneficial to health.

[0032] 3. In the present invention, the encapsulation layer is mainly composed of sodium alginate, gelatin, and resistant dextrin, which can quickly disintegrate at a temperature above 100 °C and will not significantly increase the viscosity of the decoction after disintegration.

[0033] 4. The preparation method of the dry powder preparation of the enzymatically hydrolyzed traditional Chinese medicine decoction of the present invention obtains a dry powder preparation that can stably adsorb and enrich the enzymatically hydrolyzed products of traditional Chinese medicine in an enzymatic hydrolysis environment at about 50 °C and can quickly disintegrate and release the internal enzymatically hydrolyzed products of traditional Chinese medicine in a high-pressure health-preserving decoction boiling environment above 100 °C. The dry powder preparation of the present invention will not bring solid substances with a large volume ratio after being redissolved and dispersed in the health-preserving decoction, and has little impact on the taste of the health-preserving decoction. Description of the Drawings

[0034] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and should not be regarded as limiting the scope. For those of ordinary skill in the art, without creative efforts, other related drawings can also be obtained based on these drawings, where:

[0035] Figure 1 is the surface SEM image of the modified porous yeast cell wall powder particles of the present invention. Detailed implementation manners

[0036] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention, that is, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Usually, the components of the embodiments of the present invention described and shown in the accompanying drawings here can be arranged and designed in various different configurations.

[0037] Therefore, the detailed description of the embodiments of the present invention provided in the accompanying drawings below is not intended to limit the scope of the claimed present invention, but only represents the selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0038] It should be noted that relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device. Without further limitations, an element defined by the statement "including one..." does not exclude the existence of additional identical elements in the process, method, article or device including the said element.

[0039] The features and performance of the present invention will be further described in detail below in conjunction with the embodiments.

[0040] Embodiment 1:

[0041] A dry enzyme-hydrolyzed traditional Chinese medicine decoction preparation provided by a preferred embodiment of the present invention includes traditional Chinese medicine components, enzymes and carriers, wherein the enzymes are adapted to the traditional Chinese medicine components;

[0042] The carrier includes an adsorbent and a blocking layer that wraps the adsorbent; wherein, the adsorbent is a modified porous yeast cell wall;

[0043] The modified porous yeast cell wall is a modified product obtained by subjecting a yeast cell wall to pore expansion by enzymatic hydrolysis with β-1,3-glucanase and then performing surface modification with carboxymethyl chitosan.

[0044] The modified porous yeast cell wall is prepared by the following method:

[0045] A1. At 50 °C, use 10 U / g of β-1,3-glucanase to enzymatically hydrolyze the yeast cell wall for 1.5 - 2 hours to obtain a porous yeast cell wall with micropores of 2 - 5 nm distributed therein;

[0046] A2. Dissolve carboxymethyl chitosan in MES buffer to obtain a carboxymethyl chitosan solution, add 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide to the carboxymethyl chitosan solution, and stir and react to obtain an activated carboxymethyl chitosan solution;

[0047] A3. Disperse the porous yeast cell wall in MES buffer to obtain a porous yeast cell wall suspension;

[0048] A4. Add the activated carboxymethyl chitosan solution to the porous yeast cell wall suspension, stir and react for 4 - 5 hours, then add glycine, stir for 30 - 40 minutes and then separate to obtain a precipitate, which is then washed and freeze-dried to obtain the modified porous yeast cell wall.

[0049] The specific surface area of the porous yeast cell wall after pore expansion in the present invention is significantly increased compared to that before pore expansion. As Figure 1 shown in the SEM image of the surface of the modified porous yeast cell wall powder particles, there are a large number of pore structures distributed.

[0050] The mass ratio of the porous yeast cell wall to carboxymethyl chitosan is 9 - 10:1.

[0051] The addition amount of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride is 2% of the mass of carboxymethyl chitosan, and the addition amount of N-hydroxysuccinimide is 1% of the mass of carboxymethyl chitosan.

[0052] The blocking layer includes the following components: sodium alginate, gelatin, resistant dextrin and a cross-linking agent.

[0053] The traditional Chinese medicine components include the following components: ginseng, polygonatum sibiricum, cordyceps militaris, astragalus membranaceus, and the mass ratio of ginseng, polygonatum sibiricum, cordyceps militaris, astragalus membranaceus is 4:2:2:1. The traditional Chinese medicine components in this example are one of the actually developed traditional Chinese medicine components, and the present invention is not limited to the above traditional Chinese medicine components only,

[0054] The enzyme is a complex enzyme, and the mass ratio of the complex enzyme to the traditional Chinese medicine components is 1:50 - 52. The complex enzyme includes the following components: cellulase, protease, amylase, and β-glucosidase. The pH of enzymatic hydrolysis is 5 - 6, and the mass ratio of cellulase, protease, amylase, and β-glucosidase is 1:0.5:0.5:1.

[0055] The mass ratio of resistant dextrin, sodium alginate, and gelatin is (3 - 4):(1 - 2):1.

[0056] A preparation method of an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation includes the following steps:

[0057] S1. Obtain a traditional Chinese medicine composition including ginseng, polygonatum, cordyceps militaris, and astragalus membranaceus. Boil the traditional Chinese medicine composition in water for 1 - 1.5 h, and filter to obtain a primary traditional Chinese medicine extract.

[0058] S2. Use beer yeast cell wall as a raw material, perform enzymatic treatment with β-1,3-glucanase to expand pores, and then use carboxymethyl chitosan for surface modification. After filtration and drying, obtain a modified porous yeast cell wall.

[0059] S3. Cool the primary traditional Chinese medicine extract to 50°C, adjust the pH to 5 - 6, then add cellulase, protease, amylase, and β-glucosidase. After enzymatic hydrolysis at 50°C for 2 hours, add the modified porous yeast cell wall, and continue the reaction for 1 hour. Filter to obtain a modified porous yeast cell wall loaded with enzymatically hydrolyzed traditional Chinese medicine components.

[0060] S4. Add resistant dextrin to water, stir until completely dissolved, then add sodium alginate and gelatin, and stir at 50°C for 1 h to obtain a sealing layer liquid mixture. Then disperse the modified porous yeast cell wall loaded with enzymatically hydrolyzed traditional Chinese medicine components in the sealing layer liquid mixture, stir evenly, and then dropwise add a CaCl2 cross-linking agent for cross-linking reaction. After the reaction ends, filter and spray dry to obtain a dry powder of the modified porous yeast cell wall loaded with enzymatically hydrolyzed traditional Chinese medicine components wrapped by the sealing layer, which is the enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation.

[0061] The modified porous yeast cell wall and the primary traditional Chinese medicine extract are mixed in a ratio of 15% w / v.

[0062] Example 2:

[0063] Based on Example 1, the difference from Example 1 in this example is that the modified porous yeast cell wall and the primary traditional Chinese medicine extract are mixed in a ratio of 17.5% w / v.

[0064] Example 3:

[0065] Based on Example 1, the difference in this example from Example 1 is that the modified porous yeast cell wall and the crude traditional Chinese medicine extract are mixed in a ratio of 20% w / v.

[0066] Example 4:

[0067] Based on Example 1, the present invention provides another solution of a traditional Chinese medicine composition with different functions and its corresponding enzyme. The difference from Example 1 is that the traditional Chinese medicine composition is Agaricus blazei, Angelica sinensis, and Polygonatum odoratum, the enzymes are cellulase, protease, and amylase, the enzymatic hydrolysis temperature is 50 °C, and the enzymatic hydrolysis pH is 5; the mass ratio of Agaricus blazei, Angelica sinensis, and Polygonatum odoratum is 5:3:2, and the mass ratio of cellulase, protease, and amylase is 1:0.5:1. This example is basically equivalent to Example 1 in terms of adsorption effect, disintegration time, and change in solution viscosity after disintegration.

[0068] Comparative Example 1:

[0069] Based on Example 2, the difference in this comparative example from Example 2 is that the mass ratio of the porous yeast cell wall to carboxymethyl chitosan is 8:1.

[0070] Comparative Example 2:

[0071] Based on Example 2, the difference in this comparative example from Example 2 is that the mass ratio of the porous yeast cell wall to carboxymethyl chitosan is 11:1.

[0072] Comparative Example 3:

[0073] Based on Example 2, the difference in this comparative example from Example 2 is that the carrier does not include a blocking layer. A preparation method of an enzymatic hydrolysis traditional Chinese medicine decoction dry powder preparation includes the following steps:

[0074] S1. Obtain a traditional Chinese medicine composition, including ginseng, polygonatum, cordyceps militaris, and astragalus membranaceus. Boil the traditional Chinese medicine composition in water for 1 - 1.5 h, and filter to obtain a crude traditional Chinese medicine extract;

[0075] S2. Use beer yeast wall as the raw material, perform enzymatic treatment with β-1,3-glucanase for pore expansion, and then use carboxymethyl chitosan for surface modification. After filtration and drying, obtain a modified porous yeast cell wall;

[0076] S3. Cool the crude traditional Chinese medicine extract to 50 °C, adjust the pH to 5 - 6, then add cellulase, protease, amylase, and β-glucosidase. After enzymatic hydrolysis at 50 °C for 2 hours, add the modified porous yeast cell wall and continue to react for 1 hour. Filter to obtain the modified porous yeast cell wall loaded with the enzymatic hydrolysis components of traditional Chinese medicine, and spray dry to obtain the enzymatic hydrolysis traditional Chinese medicine decoction dry powder preparation.

[0077] Comparative Example 4:

[0078] This comparative example is based on Example 2. The difference from Example 2 is that the modified porous yeast cell wall is a modified product obtained by directly performing carboxymethyl chitosan surface modification on the yeast cell wall without pore expansion by β-1,3-glucanase. The modified porous yeast cell wall is prepared by the following method:

[0079] A1. Dissolve carboxymethyl chitosan in MES buffer to obtain a carboxymethyl chitosan solution. Add 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide to the carboxymethyl chitosan solution, and stir and react to obtain an activated carboxymethyl chitosan solution;

[0080] A2. Disperse the yeast cell wall in MES buffer to obtain a yeast cell wall suspension;

[0081] A3. Add the activated carboxymethyl chitosan solution to the yeast cell wall suspension, stir and react for 4-5 hours, then add glycine, stir for 30-40 minutes and then separate to obtain a precipitate, and then wash and freeze-dry to obtain the modified porous yeast cell wall.

[0082] Comparative Example 5:

[0083] This comparative example is based on Example 2. The difference from Example 2 is that the modified porous yeast cell wall is a modified product obtained by only expanding pores of the yeast cell wall by β-1,3-glucanase without carboxymethyl chitosan surface modification.

[0084] The modified porous yeast cell wall is prepared by the following method: At 50 °C, use 10 U / g of β-1,3-glucanase to enzymatically hydrolyze the yeast cell wall, and the enzymatic hydrolysis time is 1.5-2 hours to obtain a porous yeast cell wall with 2-5 nm micropores, then wash and freeze-dry to obtain the modified porous yeast cell wall.

[0085] Comparative Example 6:

[0086] This comparative example is based on Example 2. The difference from Example 2 is that the modified porous yeast cell wall is a modified product obtained by expanding pores of the yeast cell wall by β-1,6-glucanase and then performing carboxymethyl chitosan surface modification. The method for pore expansion by enzymatic hydrolysis refers to the existing usage conditions of β-1,6-glucanase, and the enzymatic hydrolysis temperature is 55 °C.

[0087] Comparative Example 7:

[0088] This comparative example is based on Example 2. The difference from Example 2 is that the modified porous yeast cell wall is a modified product obtained by surface modification with carboxymethyl chitosan after pore expansion by enzymatic hydrolysis with cellulase. The method of pore expansion by enzymatic hydrolysis refers to the existing usage conditions of cellulase. If the pore size is too large, it cannot prevent the adsorption of macromolecules and target the adsorption of small molecules.

[0089] Comparative Example 8:

[0090] This comparative example is based on Example 2. The difference from Example 2 is that the adsorbent is mesoporous silica. A preparation method of an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation includes the following steps:

[0091] S1. Obtain a traditional Chinese medicine composition including ginseng, polygonatum sibiricum, cordyceps militaris, and astragalus membranaceus. Boil the traditional Chinese medicine composition in water for 1 - 1.5 h, and filter to obtain a primary traditional Chinese medicine extract;

[0092] S2. Cool the primary traditional Chinese medicine extract to 50 °C, adjust the pH to 5 - 6, then add cellulase, protease, amylase, and β - glucosidase, carry out enzymatic hydrolysis at 50 °C for 2 hours, add mesoporous silica, continue the reaction for 1 hour, and filter to obtain mesoporous silica loaded with enzymatically hydrolyzed traditional Chinese medicine components;

[0093] S4. Add resistant dextrin to water and stir until completely dissolved, then add sodium alginate and gelatin, stir at a constant temperature of 50 °C for 1 h to obtain a sealing layer liquid mixture. Then disperse the mesoporous silica loaded with enzymatically hydrolyzed traditional Chinese medicine components in the sealing layer liquid mixture, stir evenly, and then dropwise add a CaCl2 cross - linker for cross - linking reaction. After the reaction ends, filter and spray - dry to obtain mesoporous silica dry powder loaded with enzymatically hydrolyzed traditional Chinese medicine components wrapped by the sealing layer, which is the enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation.

[0094] Comparative Example 9:

[0095] This comparative example is based on Example 2. The difference from Example 2 is that the sealing layer does not include resistant dextrin.

[0096] Test Example 1:

[0097] Detect the adsorption rates of the adsorbents in Examples 1 - 3 and Comparative Examples 1 - 9 for ginsenoside and cordycepin in a test environment with a pH of 5 - 6 and a temperature of 50 °C, and detect the disintegration time of the enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparations prepared in Examples 1 - 3 and Comparative Examples 1 - 9 in an aqueous solution under a pressure of 120 °C and the solution viscosity after disintegration; the results are shown in Table 1. The detection methods for adsorption rate, disintegration time, and solution viscosity are all existing technologies and will not be elaborated here;

[0098] Table 1 Performance detection of adsorbents and enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparations:

[0099] 。

[0100] Test Example 2:

[0101] Detect the storage time and the toxicity after disintegration of the enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparations prepared in Examples 1-4 and Comparative Examples 1-9. The detection results are shown in Table 2.

[0102] The detection method for the storage time is as follows: Seal the dry powder in an aluminum foil bag (humidity < 30%), take samples for detection every month, and detect the retention rate of active ingredients (HPLC method): such as the change in the peak area of ginsenosides, cordycepin, etc., and the microbial limit (GB 4789.2): It should meet the food standard (total number of colonies < 1,000 CFU / g); Endpoint determination: Terminate when the retention rate of active ingredients < 90% or the microorganisms exceed the standard;

[0103] Acute toxicity (rat experiment): Refer to GB 15193.3-2014 "National Food Safety Standard Acute Oral Toxicity Test".

[0104] Table 2 Detection results of the storage stability and toxicity of the enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation:

[0105] 。

[0106] Combined with the data in Table 1 and Table 2, it can be seen that Example 2 of the present invention is the best solution.

[0107] The above are only the preferred embodiments of the present invention and are not intended to limit the protection scope of the present invention. Any modifications, equivalent replacements, and improvements made by those skilled in the art within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. An enzyme-hydrolyzed traditional Chinese medicine decoction dry powder preparation, characterized in that: It includes traditional Chinese medicine components, enzymes and carriers, where the enzymes are adapted to the traditional Chinese medicine components; The carrier includes an adsorbent and a blocking layer that wraps the adsorbent; among them, the adsorbent is a modified porous yeast cell wall; The modified porous yeast cell wall is a modified product obtained by enzymatically expanding the pores of the yeast cell wall with β-1,3-glucanase and then performing carboxymethyl chitosan surface modification; The modified porous yeast cell wall is prepared by the following method: A1. At 50 °C, use 10 U / g of β-1,3-glucanase to enzymatically hydrolyze the yeast cell wall, and the enzymatic hydrolysis time is 1.5 - 2 hours to obtain a porous yeast cell wall with 2 - 5 nm micropores distributed; A2. Dissolve carboxymethyl chitosan in MES buffer to obtain a carboxymethyl chitosan solution, add 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide to the carboxymethyl chitosan solution, and stir and react to obtain an activated carboxymethyl chitosan solution; A3. Disperse the porous yeast cell wall in MES buffer to obtain a porous yeast cell wall suspension; A4. Add the activated carboxymethyl chitosan solution to the porous yeast cell wall suspension, stir and react for 4 - 5 hours, then add glycine, stir for 30 - 40 minutes and then separate to obtain a precipitate, and then wash and freeze-dry to obtain the modified porous yeast cell wall; The mass ratio of the porous yeast cell wall to carboxymethyl chitosan is 9 - 10:1; The addition amount of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride is 2% of the mass of carboxymethyl chitosan, and the addition amount of N-hydroxysuccinimide is 1% of the mass of carboxymethyl chitosan; The blocking layer includes the following components: sodium alginate, gelatin, resistant dextrin and a cross-linking agent; The mass ratio of resistant dextrin, sodium alginate to gelatin is (3 - 4):(1 - 2):

1.

2. The dry powder preparation of enzymatically hydrolyzed traditional Chinese medicine decoction according to claim 1, wherein: The traditional Chinese medicine components include the following components: ginseng, polygonatum sibiricum, cordyceps militaris, astragalus membranaceus, and the mass ratio of ginseng, polygonatum sibiricum, cordyceps militaris, astragalus membranaceus is 4:2:2:

1.

3. The dry powder preparation of enzymatically hydrolyzed traditional Chinese medicine decoction according to claim 2, characterized in that: The enzyme adapted to the traditional Chinese medicine components is a composite enzyme, and the mass ratio of the composite enzyme to the traditional Chinese medicine components is 1:50 - 52. The composite enzyme includes the following components: cellulase, protease, amylase, β-glucosidase, the enzymatic hydrolysis pH is 5 - 6, and the mass ratio of cellulase, protease, amylase, β-glucosidase is 1:0.5:0.5:

1.

4. The preparation method of an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation according to any one of claims 1-3, characterized in that: It includes the following steps: S1. Obtain traditional Chinese medicine components, including ginseng, polygonatum sibiricum, cordyceps militaris, astragalus membranaceus, boil the traditional Chinese medicine components in water for 1 - 1.5 h, and filter to obtain a crude traditional Chinese medicine extract; S2. Use beer yeast wall as raw material, perform enzymatic treatment for pore expansion with β-1,3-glucanase and then perform surface modification with carboxymethyl chitosan, filter and dry to obtain the modified porous yeast cell wall; S3. Cool the crude traditional Chinese medicine extract to 50 °C, adjust the pH to 5 - 6, then add cellulase, protease, amylase, β-glucosidase, enzymatically hydrolyze at 50 °C for 2 hours, then add the modified porous yeast cell wall, continue to react for 1 hour, and filter to obtain the modified porous yeast cell wall loaded with enzymatically hydrolyzed components of traditional Chinese medicine; S4. Add resistant dextrin into water, stir until it is completely dissolved, then add sodium alginate and gelatin, and stir at a constant temperature of 50 °C for 1 h to obtain a liquid mixture of the blocking layer. Then disperse the modified porous yeast cell wall loaded with the enzymatically hydrolyzed components of traditional Chinese medicine in the liquid mixture of the blocking layer, stir evenly, and then dropwise add a CaCl2 cross-linking agent for cross-linking reaction. After the reaction is completed, filter and spray dry to obtain the dry powder of the modified porous yeast cell wall loaded with the enzymatically hydrolyzed components of traditional Chinese medicine wrapped by the blocking layer, which is the dry powder preparation of the enzymatically hydrolyzed traditional Chinese medicine decoction.

5. The preparation method of an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation according to claim 4, characterized in that: The modified porous yeast cell wall and the crude extract of traditional Chinese medicine are mixed in a ratio of 15 - 20% w / v.

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