Efficient affinity chromatography bilirubin extraction device

Abstract

The invention relates to the technical field of bilirubin extraction, in particular to an efficient affinity chromatography bilirubin extraction device. Comprising an extraction device body, and a circulating chromatography mechanism is arranged at the top of the extraction device body. Ligands in the adjustable chromatography assemblies are used for adsorbing bilirubin in animal bile, then waste liquid enters the waste liquid tank, the rotating column is controlled to rotate after the corresponding adjustable chromatography assembly is saturated, the next adjustable chromatography assembly continues to adsorb bilirubin, when the adjustable chromatography assemblies rotate to the corresponding positions, an eluent can be guided in, and then the bilirubin in the animal bile can be eluted. During adsorption and elution, bilirubin is released and enters the purification tank, and in the circulation process of adsorption and elution, the adjustable chromatography assembly can fully drain the internal solution and discharge the solution into the waste liquid tank or the purification tank through the corresponding liquid guide tank, so that different solutions are prevented from being mixed with one another; while the bilirubin extraction efficiency of the extraction device is improved, the bilirubin extraction quality is also improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of bilirubin extraction, and particularly relates to a high-efficiency affinity chromatography bilirubin extraction device. Background Art

[0002] Bilirubin is usually extracted from animal bile. Bilirubin is an organic compound with important biological functions and can be used in industrial production.

[0003] After retrieval, in the prior art, Chinese Patent Publication No.: CN221155460U, Publication Date: June 18, 2024, discloses a bilirubin extraction device, including an extraction tank. A base is fixedly installed at the bottom end of the extraction tank. A stirring tank is fixedly installed inside the extraction tank. A sealing cover is installed at the top end of the extraction tank. An air inlet pipe is fixedly installed in the middle of the top end of the sealing cover. A feed pipe is fixedly installed at the edge of the top end of the sealing cover. Through the settings of the rotating frame, stirring rod B, annular internal gear, and gear A, the rotation of the annular internal gear drives the rotation of the rotating frame, so that the stirring rod B moves along the inner wall of the stirring tank for stirring, making the stirring inside the stirring tank more uniform. The uniform stirring ensures that bilirubin and the extraction reagent in the sample are fully mixed and reacted, thereby improving the accuracy and reliability of the measurement results, and can reduce the waste of reagents for bilirubin extraction because each sample can fully react, thus saving time and cost.

[0004] However, the following defects still exist in this extraction device:

[0005] In the prior art, the methods for extracting bilirubin usually adopt the chloroform extraction method and the calcium bilirubinate method. However, in the above extraction methods, there are usually time-consuming processes during the treatment, such as saponification, acidification, and extraction processes, thereby reducing the extraction efficiency of bilirubin. Summary of the Invention

[0006] In view of the above problems, the present invention provides a high-efficiency affinity chromatography bilirubin extraction device, including an extraction device body. Waste liquid tanks and purification tanks are symmetrically arranged at the top of the extraction device body. A raw material storage tank and an eluent storage tank are respectively arranged at one side edge of the extraction device body.

[0007] A bile filtration mechanism is arranged at the top of the raw material storage tank. A circulating chromatography mechanism is arranged at the top of the extraction device body. The top of the circulating chromatography mechanism is movably communicated with the raw material storage tank and the eluent storage tank, and the bottom is communicated with the waste liquid tank and the purification tank.

[0008] The cyclic chromatography mechanism includes a bottom plate for support and a top plate for connection; a rotating column for providing cyclic rotation for cyclic chromatography is arranged between the top plate and the bottom plate; a plurality of groups of mounting grooves for mounting the chromatography structure are arranged at the top of the rotating column in an annular array distribution;

[0009] A set of adjustable chromatography components capable of adjusting the solution channel according to requirements are movably penetrated through each group of mounting grooves; two groups of liquid guide grooves for guiding the residual solution and arranged obliquely are symmetrically arranged on the top of the bottom plate in a rotationally symmetric manner.

[0010] Further, an electric push rod is arranged on one side wall of the extraction device body; a lifting plate is drivingly connected to the output end of the electric push rod; two connecting pipes are symmetrically arranged at the bottom of the end of the lifting plate away from the electric push rod; the two connecting pipes are respectively communicated with the raw material storage tank and the eluent storage tank; two groups of sliding grooves are symmetrically arranged on the top of the extraction device body; a limiting block is arranged in each group of sliding grooves; each group of limiting blocks movably penetrates through the cyclic chromatography mechanism.

[0011] Further, a transmission cavity is arranged inside the extraction device body; two screw rods are arranged in the transmission cavity; one ends of the two screw rods are fixedly connected, and the other ends are respectively rotatably connected to the inner walls on both sides of the transmission cavity; the thread directions of the two screw rods are opposite, and the central axes are on the same straight line; two sliders are slidably connected to the inner wall of the bottom of the transmission cavity; each slider is in threaded connection with a corresponding screw rod; each slider is drivingly connected to a corresponding limiting block.

[0012] Further, the bile filtration mechanism includes a box body; a centralized groove is arranged at the top of the box body; a collection box is movably penetrated through one side wall of the box body; a second motor is arranged on one side wall of the box body; the output end of the second motor extends into the interior of the box body and is drivingly connected to a rotating shaft; a filter sleeve is sleeved on the outer wall of the rotating shaft; a plurality of groups of liquid guide channels are arranged at equal intervals in the filter sleeve.

[0013] Further, a plurality of groups of filter holes are arranged on the outer side inner wall of the liquid guide channel in an annular array distribution; a liquid blocking plate is arranged on one side inner wall of the box body; the liquid blocking plate is movably attached to the outer wall of the filter sleeve; a scraping plate and an extrusion block are arranged in the collection box; the extrusion block is located directly below the filter sleeve and is movably abutted against the outer wall of the filter sleeve; the scraping plate is located on the side of the extrusion block away from the centralized groove and is movably attached to the outer wall of the filter sleeve.

[0014] Further, a plurality of groups of connecting rods are arranged on the top of the bottom plate in an annular array distribution; the tops of the plurality of groups of connecting rods are clamped with the top plate; two groups of limiting grooves are symmetrically arranged on the outer wall of the bottom plate; each group of limiting blocks movably penetrates through a corresponding group of limiting grooves.

[0015] Furthermore, two sets of drain pipes are provided at the bottom of the bottom plate; the two sets of drain pipes are respectively communicated with the waste liquid tank and the purification tank; two sets of liquid inlet pipes are provided at the top of the top plate; each set of liquid inlet pipes is communicated with a corresponding set of connecting pipes; a third motor is provided at the center of the top of the top plate; the output end of the third motor is in transmission connection with the rotating column; the lowest point of each set of liquid guide grooves is communicated with a corresponding set of drain pipes.

[0016] Furthermore, the adjustable chromatography assembly includes a chromatography tube; a first baffle is movably penetrated through the top of the chromatography tube; a rotating block is rotatably connected to the top of the first baffle; two sets of clamping blocks are symmetrically provided at the bottom of the first baffle.

[0017] Furthermore, a second baffle is rotatably connected to the bottom of the chromatography tube; a plurality of liquid through holes are uniformly distributed on both the first baffle and the second baffle; a flow disturbing plate is provided at the top of the second baffle; a plurality of communication holes are distributed in a rectangular array on the flow disturbing plate.

[0018] Furthermore, one end of the rotating block extends into the first baffle and is in transmission connection with a gear; a set of racks that can only slide horizontally are respectively meshed on both sides of the gear; each set of racks is in transmission connection with a corresponding set of clamping blocks.

[0019] The beneficial effects of the present invention are:

[0020] 1. By using the ligand packing in the adjustable chromatography assembly to adsorb bilirubin in animal bile, then the waste liquid enters the waste liquid tank. After the corresponding adjustable chromatography assembly is saturated, the rotating column is controlled to rotate, so that the next set of adjustable chromatography assemblies continues to adsorb bilirubin. When the adjustable chromatography assembly rotates to the corresponding position, the eluent can be introduced to release bilirubin and enter the purification tank. And during the adsorption and elution cycle process, the adjustable chromatography assembly can fully drain the internal solution and discharge it into the waste liquid tank or the purification tank through the corresponding liquid guide grooves, avoiding the mixing of different solutions, and improving the bilirubin extraction efficiency of the extraction device while also improving the extraction quality of bilirubin.

[0021] 2. By placing the ligand packing for adsorbing bilirubin on both sides of the flow disturbing plate in the chromatography tube, then placing the first baffle on the top of the chromatography tube and rotating the rotating block, the rotating block drives the gear to rotate. Under the meshing connection relationship between the gear and the two sets of racks, the two sets of racks drive the two sets of clamping blocks to clamp the top of the flow disturbing plate. While fixing the first baffle, the flow disturbing plate can be driven to generate torsion by continuously rotating the rotating block, so that the animal bile can generate a refraction process when entering the chromatography tube, thereby enabling the ligand packing to fully adsorb bilirubin and improving the chromatography effect of the extraction device.

[0022] 3. The filter sleeve is driven to rotate slowly in the direction opposite to the falling direction of the animal bile by controlling the rotating shaft. The animal bile enters the liquid guide channel after filtering the impurities through a plurality of filter holes on the surface of the filter sleeve, and is discharged from the corresponding filter holes below under the action of gravity. The grease in the animal bile is absorbed by the filter sleeve, and the impurities attached to the surface of the filter sleeve are scraped off by the scraper and collected separately. Then the squeezing block squeezes the filter sleeve to squeeze out the grease absorbed in the filter sleeve and collect it separately, which not only improves the raw material processing efficiency of the extraction device, but also can separate the impurities and grease, thereby improving the raw material processing effect of the extraction device.

[0023] 4. The electric push rod is controlled to drive the two sets of connecting tubes to rise and disconnect the connection with the circulating chromatography mechanism, and then the first motor is controlled to drive the two sets of screw rods to rotate. Under the threaded connection relationship between the two sets of screw rods and the two sets of sliders, the two sets of sliders drive the two sets of limit blocks to move toward opposite sides and disengage from the circulating chromatography mechanism, so that the circulating chromatography mechanism can be directly disassembled from the extraction device body for repair or maintenance, thereby improving the maintenance efficiency of the extraction device.

[0024] Other features and advantages of the present invention will be described in the following description, and partly become apparent from the description, or understood by practicing the present invention. The purpose and other advantages of the present invention can be realized and obtained by the structures pointed out in the description, claims and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0026] Figure 1 A schematic diagram of the structure of an extraction device according to an embodiment of the present invention is shown;

[0027] Figure 2 A schematic top view of a cross-sectional view of an extraction device according to an embodiment of the present invention is shown;

[0028] Figure 3 A schematic structural diagram of a bile filtering mechanism according to an embodiment of the present invention is shown;

[0029] Figure 4 A cross-sectional schematic diagram of a bile filtering mechanism according to an embodiment of the present invention is shown;

[0030] Figure 5 It shows a schematic structural diagram of a circulating chromatography mechanism according to an embodiment of the present invention;

[0031] Figure 6 Shows an exploded schematic view of a cyclic chromatography mechanism according to an embodiment of the present invention;

[0032] Figure 7 Shows a structural schematic view of an adjustable chromatography assembly according to an embodiment of the present invention;

[0033] Figure 8 Shows a cross-sectional schematic view of an adjustable chromatography assembly according to an embodiment of the present invention;

[0034] Figure 9 Shows a top-down cross-sectional schematic view of a first baffle according to an embodiment of the present invention.

[0035] In the figure: 1, the main body of the extraction device; 2, the sealing cover; 3, the raw material storage tank; 4, the bile filtration mechanism; 5, the eluent storage tank; 6, the electric push rod; 7, the lifting plate; 8, the connecting pipe; 9, the cyclic chromatography mechanism; 10, the chute; 11, the limit block; 12, the waste liquid tank; 13, the purification tank; 14, the transmission cavity; 15, the lead screw; 16, the slider; 17, the first motor; 401, the box body; 402, the centralized tank; 403, the collection box; 404, the second motor; 405, the scraper; 406, the extrusion block; 407, the rotating shaft; 408, the filter sleeve; 409, the liquid guiding channel; 410, the filter hole; 411, the liquid blocking plate; 901, the bottom plate; 902, the connecting rod; 903, the top plate; 904, the rotating column; 905, the limit groove; 906, the drain pipe; 907, the liquid inlet pipe; 908, the third motor; 909, the installation groove; 910, the adjustable chromatography assembly; 911, the liquid guiding groove; 9101, the chromatography tube; 9102, the first baffle; 9103, the rotating block; 9104, the clamping block; 9105, the second baffle; 9106, the flow disturbing plate; 9107, the communication hole; 9108, the gear; 9109, the rack. Detailed implementation manners

[0036] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Apparently, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0037] The embodiments of the present invention provide an efficient affinity chromatography bilirubin extraction device, including the main body 1 of the extraction device. Exemplarily, such as Figure 1 and Figure 2As shown, a waste liquid tank 12 and a purification tank 13 are symmetrically provided on the top of the extraction device body 1; a sealing cover 2 is provided on the top of each of the waste liquid tank 12 and the purification tank 13; a raw material storage box 3 and an eluent storage box 5 are respectively provided at one side edge of the extraction device body 1; a bile filtering mechanism 4 is provided on the top of the raw material storage box 3; an electric push rod 6 is provided on one side wall of the extraction device body 1; a lifting plate 7 is transmission-connected to the output end of the electric push rod 6; two groups of connecting pipes 8 are symmetrically provided at the bottom of one end of the lifting plate 7 away from the electric push rod 6; the two groups of connecting pipes 8 are respectively connected to the raw material storage box 3 and the eluent storage box 5; a circulating chromatography mechanism 9 is provided on the top of the extraction device body 1; the top of the circulating chromatography mechanism 9 is movably connected to the two groups of connecting pipes 8, and the bottom is connected to the waste liquid tank 12 and the purification tank 13; the extraction device body 1 is symmetrically provided with two groups of slide grooves 10 at the top; each group of the slide grooves 10 is provided with a group of limit blocks 11; each group of the limit blocks 11 is movable and runs through the circulating chromatography mechanism 9; a transmission chamber 14 is provided in the extraction device body 1; two groups of screw rods 15 are provided in the transmission chamber 14; one end of the two groups of screw rods 15 is fixedly connected, and the other end is rotatably connected to the inner walls on both sides of the transmission chamber 14; the thread directions of the two groups of screw rods 15 are opposite, and the central axes are on the same straight line; two groups of sliders 16 are slidably connected to the bottom inner wall of the transmission chamber 14; each group of the sliders 16 is threadedly connected to the corresponding group of screw rods 15; each group of the sliders 16 is transmission-connected to the corresponding group of limit blocks 11; a first motor 17 is provided on one side wall of the extraction device body 1; the output end of the first motor 17 is transmission-connected to one of the groups of screw rods 15.

[0038] When performing affinity chromatography extraction of bilirubin, animal bile is first added to the raw material storage tank 3 through the bile filtering mechanism 4, and then the filtered animal bile is introduced into the circulating chromatography mechanism 9. The circulating chromatography mechanism 9 is provided with a ligand filler for adsorbing bilirubin. The bilirubin in the bile solution is adsorbed by the ligand filler, and then the waste liquid enters the waste liquid tank 12. Then the eluent in the eluent storage tank 5 is introduced into the circulating chromatography mechanism 9 to release the bilirubin adsorbed by the ligand filler and bring the bilirubin into the purification tank 13 to facilitate subsequent processing. When the circulation chromatography mechanism 9 fails, the electric push rod 6 is first controlled to drive the two groups of connecting pipes 8 to rise and disconnect the circulation chromatography mechanism 9, and then the first motor 17 is controlled to drive the two groups of screw rods 15 to rotate. Under the threaded connection relationship between the two groups of screw rods 15 and the two groups of sliders 16, the two groups of sliders 16 drive the two groups of limit blocks 11 to move toward opposite sides and disengage from the circulation chromatography mechanism 9, so that the circulation chromatography mechanism 9 can be directly disassembled from the extraction device body 1 for repair or maintenance, thereby improving the maintenance efficiency of the extraction device.

[0039] For example, Figure 3 andFigure 4 As shown, the bile filtration mechanism 4 includes a box body 401; a centralized tank 402 is provided at the top of the box body 401; a collection box 403 penetrates through a side wall of the box body 401 movably; a second motor 404 is provided on a side wall of the box body 401; an output end of the second motor 404 extends into the interior of the box body 401 and is drivingly connected to a rotating shaft 407; a filter sleeve 408 is sleeved on an outer wall of the rotating shaft 407; the filter sleeve 408 is made of an elastic oil-loving and water-repellent material; a plurality of groups of liquid guiding channels 409 are arranged at equal intervals in the filter sleeve 408; a plurality of groups of filter holes 410 are arranged in a circumferential array on an outer side inner wall of the liquid guiding channel 409; a liquid blocking plate 411 is provided on a side inner wall of the box body 401; the liquid blocking plate 411 is movably attached to an outer wall of the filter sleeve 408; a scraping plate 405 and a pressing block 406 are provided in the collection box 403; the pressing block 406 is located directly below the filter sleeve 408 and is movably abutted against the outer wall of the filter sleeve 408; the scraping plate 405 is located on a side of the pressing block 406 away from the centralized tank 402 and is movably attached to the outer wall of the filter sleeve 408.

[0040] During the process of adding animal bile into the raw material storage tank 3, the animal bile enters the interior of the box body 401 through the centralized tank 402 and falls under the action of gravity in a direction deviating from the axis of the filter sleeve 408. The rotating shaft 407 drives the filter sleeve 408 to slowly rotate in a direction opposite to the falling direction of the animal bile. The animal bile filters out impurities through a plurality of groups of filter holes 410 on the surface of the filter sleeve 408 and enters the liquid guiding channels 409, and then is discharged into the raw material storage tank 3 through the corresponding filter holes 410 below under the action of gravity. Moreover, the oil in the animal bile is absorbed by the filter sleeve 408. The filter sleeve 408 continues to rotate, and the scraping plate 405 scrapes off the impurities attached to the surface of the filter sleeve 408 and collects them separately. Subsequently, the pressing block 406 presses the filter sleeve 408 to squeeze out and collect separately the oil absorbed in the filter sleeve 408. This not only improves the raw material processing efficiency of the extraction device but also can separate impurities and oil, improving the raw material processing effect of the extraction device.

[0041] Exemplarily, such as Figure 5 and Figure 6As shown in the figure, the cyclic chromatography mechanism 9 includes a bottom plate 901; a plurality of groups of connecting rods 902 are arranged in a circular array distribution on the top of the bottom plate 901; the tops of the plurality of groups of connecting rods 902 are clamped with a top plate 903; a rotating column 904 is arranged between the top plate 903 and the bottom plate 901; two groups of limiting grooves 905 are symmetrically opened on the outer wall of the bottom plate 901; each group of limiting blocks 11 movably penetrates through a corresponding group of limiting grooves 905; two groups of drain pipes 906 are arranged at the bottom of the bottom plate 901; the two groups of drain pipes 906 are respectively communicated with the waste liquid tank 12 and the purification tank 13; two groups of liquid inlet pipes 907 are arranged on the top of the top plate 903; each group of liquid inlet pipes 907 is communicated with a corresponding group of connecting pipes 8; a third motor 908 is arranged at the center of the top of the top plate 903; the output end of the third motor 908 is in transmission connection with the rotating column 904; a plurality of groups of installation grooves 909 are arranged in a circular array distribution on the top of the rotating column 904; a group of adjustable chromatography components 910 movably penetrates through each group of installation grooves 909; two groups of inclined liquid guide grooves 911 are symmetrically opened on the top of the bottom plate 901; the lowest part of each group of liquid guide grooves 911 is communicated with a corresponding group of drain pipes 906.

[0042] When performing the chromatography extraction work of bilirubin, animal bile enters a corresponding group of adjustable chromatography components 910 through one of the liquid inlet pipes 907. The ligand packing in the adjustable chromatography components 910 adsorbs bilirubin in the animal bile. Subsequently, the waste liquid enters the waste liquid tank 12 through a corresponding group of drain pipes 906. After the corresponding adjustable chromatography components 910 are saturated, the third motor 908 drives the rotating column 904 to rotate, and the next group of adjustable chromatography components 910 continues to adsorb bilirubin in the animal bile. When the adjustable chromatography components 910 that have adsorbed bilirubin rotate below the other group of liquid inlet pipes 907, the eluent can be introduced to release bilirubin and enter the purification tank 13. And during the adsorption and elution cycle process, the adjustable chromatography components 910 can fully drain the internal solution and discharge it into the waste liquid tank 12 or the purification tank 13 through the corresponding liquid guide grooves 911, avoiding the mixing of different solutions, improving the bilirubin extraction efficiency of the extraction device and also improving the extraction quality of bilirubin.

[0043] Exemplarily, such as Figure 7 、 Figure 8 and Figure 9As shown in the figure, the adjustable chromatography assembly 910 includes a chromatography tube 9101; a first baffle 9102 movably penetrates through the top of the chromatography tube 9101; a rotating block 9103 is rotatably connected to the top of the first baffle 9102; two groups of clamping blocks 9104 are symmetrically arranged at the bottom of the first baffle 9102; a second baffle 9105 is rotatably connected to the bottom of the chromatography tube 9101; a number of liquid through holes are uniformly distributed on both the first baffle 9102 and the second baffle 9105; a spoiler 9106 is arranged on the top of the second baffle 9105; the spoiler 9106 is made of a plastic metal material; a number of communication holes 9107 are arranged in a rectangular array on the spoiler 9106; one end of the rotating block 9103 extends into the first baffle 9102 and is drivingly connected to a gear 9108; two groups of racks 9109 that can only slide horizontally are respectively meshed with both sides of the gear 9108; each group of racks 9109 is drivingly connected to a corresponding group of clamping blocks 9104.

[0044] By placing the ligand packing for adsorbing bilirubin on both sides of the spoiler 9106 in the chromatography tube 9101, then placing the first baffle 9102 on the top of the chromatography tube 9101 and rotating the rotating block 9103, the rotating block 9103 drives the gear 9108 to rotate. Under the meshing connection relationship between the gear 9108 and the two groups of racks 9109, the two groups of racks 9109 drive the two groups of clamping blocks 9104 to clamp the top of the spoiler 9106. While fixing the first baffle 9102, the spoiler 9106 can be driven to twist by continuously rotating the rotating block 9103, so that when animal bile enters the chromatography tube 9101, a refraction process can occur, enabling the ligand packing to fully adsorb bilirubin and improving the chromatography effect of the extraction device.

[0045] By using the ligand packing in the adjustable chromatography assembly 910 to adsorb bilirubin in animal bile, and then the waste liquid enters the waste liquid tank 12. After the corresponding adjustable chromatography assembly 910 is saturated, the rotating column 904 is controlled to rotate, so that the next group of adjustable chromatography assemblies 910 continues to adsorb bilirubin. When the adjustable chromatography assembly 910 rotates to the corresponding position, the eluent can be introduced to release bilirubin and enter the purification tank 13. And during the adsorption and elution cycle process, the adjustable chromatography assembly 910 can fully drain the internal solution and discharge it into the waste liquid tank 12 or the purification tank 13 through the corresponding liquid guide groove 911, avoiding the mixing of different solutions, and improving both the bilirubin extraction efficiency and the bilirubin extraction quality of the extraction device.

[0046] By placing the ligand filler for adsorbing bilirubin on both sides of the spoiler 9106 in the chromatography tube 9101, and then placing the first baffle 9102 on the top of the chromatography tube 9101, and rotating the rotating block 9103, the rotating block 9103 drives the gear 9108 to rotate. Under the meshing connection relationship between the gear 9108 and the two sets of racks 9109, the two sets of racks 9109 drive the two sets of clamping blocks 9104 to clamp the top of the spoiler 9106. While fixing the first baffle 9102, the spoiler 9106 can be twisted by continuing to rotate the rotating block 9103, so that the animal bile can be refracted after entering the chromatography tube 9101, so that the ligand filler can fully adsorb bilirubin, thereby improving the chromatography effect of the extraction device.

[0047] The filter sleeve 408 is driven to rotate slowly in the direction opposite to the falling direction of the animal bile by controlling the rotating shaft 407. The animal bile passes through a plurality of groups of filter holes 410 on the surface of the filter sleeve 408 to filter out impurities and enter the liquid guide channel 409, and is discharged from the corresponding filter holes 410 below under the action of gravity. The grease in the animal bile is absorbed by the filter sleeve 408, and the impurities attached to the surface of the filter sleeve 408 are scraped off by the scraper 405 and collected separately. Then, the squeezing block 406 squeezes the filter sleeve 408, squeezes out the grease absorbed in the filter sleeve 408 and collects it separately, which not only improves the raw material processing efficiency of the extraction device, but also can separate impurities and grease, thereby improving the raw material processing effect of the extraction device.

[0048] The electric push rod 6 is controlled to drive the two groups of connecting tubes 8 to rise and disconnect the connection with the circulating chromatography mechanism 9, and then the first motor 17 is controlled to drive the two groups of screw rods 15 to rotate. Under the threaded connection relationship between the two groups of screw rods 15 and the two groups of sliders 16, the two groups of sliders 16 drive the two groups of limit blocks 11 to move toward opposite sides and disengage from the circulating chromatography mechanism 9, so that the circulating chromatography mechanism 9 can be directly disassembled from the extraction device body 1 for repair or maintenance, thereby improving the maintenance efficiency of the extraction device.

[0049] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein; and these modifications or substitutions do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A high-efficiency affinity chromatography bilirubin extraction device, comprising an extraction device body, characterized in that: A waste liquid tank and a purification tank are symmetrically provided on the top of the extraction device body; a raw material storage box and an eluent storage box are respectively provided on one side edge of the extraction device body; The top of the raw material storage tank is provided with a bile filtering mechanism; the top of the extraction device body is provided with a circulating chromatography mechanism; the top of the circulating chromatography mechanism is movably connected with the raw material storage tank and the eluent storage tank, and the bottom is connected with the waste liquid tank and the purification tank; The circulating chromatography mechanism comprises a bottom plate for support and a top plate for connection; a rotating column for providing circulating rotation for the circulating chromatography is arranged between the top plate and the bottom plate; a plurality of groups of mounting grooves for mounting the chromatography structure are arranged on the top of the rotating column in a circular array; Each group of the installation grooves is movably penetrated by a group of adjustable chromatography components capable of adjusting the solution channel according to demand; the top of the bottom plate is rotationally symmetrically provided with two groups of inclined liquid guide grooves for guiding residual solution.

2. The high-efficiency affinity chromatography bilirubin extraction device according to claim 1, characterized in that: An electric push rod is provided on one side wall of the extraction device body; a lifting plate is transmission-connected on the output end of the electric push rod; two groups of connecting pipes are symmetrically provided at the bottom of one end of the lifting plate away from the electric push rod; the two groups of connecting pipes are respectively connected to the raw material storage box and the eluent storage box; two groups of slide grooves are symmetrically opened on the top of the extraction device body; a group of limit blocks is provided in each group of the slide grooves; and each group of the limit blocks is movable and runs through the circulating chromatography mechanism.

3. The high-efficiency affinity chromatography bilirubin extraction device according to claim 2, characterized in that: A transmission chamber is provided in the extraction device body; two groups of screw rods are provided in the transmission chamber; one end of the two groups of screw rods are fixedly connected, and the other ends are rotatably connected to the inner walls on both sides of the transmission chamber; the thread directions of the two groups of screw rods are opposite, and the central axes are on the same straight line; two groups of sliders are slidably connected to the bottom inner wall of the transmission chamber; each group of sliders is threadedly connected to a corresponding group of screw rods; each group of sliders is transmission connected to a corresponding group of limit blocks.

4. The high-efficiency affinity chromatography bilirubin extraction device according to claim 1, characterized in that: The bile filtering mechanism comprises a box body; a concentration groove is arranged on the top of the box body; a collecting box is movably penetrated on one side wall of the box body; a second motor is arranged on one side wall of the box body; an output end of the second motor extends to the inside of the box body and is connected to a rotating shaft in a transmission manner; a filter sleeve is sleeved on the outer wall of the rotating shaft; and a plurality of groups of liquid guide channels are arranged at equal intervals in the filter sleeve.

5. The high-efficiency affinity chromatography bilirubin extraction device according to claim 4, characterized in that: A plurality of groups of filter holes are arranged in a circular array on the inner wall of the outer side of the liquid guiding channel; a liquid baffle is arranged on the inner wall of one side of the box body; the liquid baffle is movably fitted on the outer wall of the filter sleeve; a scraper and an extrusion block are arranged in the collecting box; the extrusion block is located directly below the filter sleeve and movably abuts against the outer wall of the filter sleeve; the scraper is located on the side of the extrusion block away from the focusing groove and movably fits on the outer wall of the filter sleeve.

6. The high-efficiency affinity chromatography bilirubin extraction device according to claim 3, characterized in that: A plurality of connecting rods are arranged in a circular array on the top of the bottom plate; the tops of the plurality of connecting rods are clamped with the top plate; two groups of limiting grooves are symmetrically opened on the outer wall of the bottom plate; each group of limiting blocks is movable through a corresponding group of limiting grooves.

7. The high-efficiency affinity chromatography bilirubin extraction device according to claim 6, characterized in that: Two groups of drainage pipes are provided at the bottom of the bottom plate; the two groups of drainage pipes are connected to the waste liquid tank and the purification tank respectively; two groups of liquid inlet pipes are provided at the top of the top plate; each group of liquid inlet pipes is connected to a corresponding group of connecting pipes; a third motor is provided at the top center of the top plate; the output end of the third motor is transmission-connected to the rotating column; the lowest point of each group of liquid guiding grooves is connected to a corresponding group of drainage pipes.

8. The high-efficiency affinity chromatography bilirubin extraction device according to claim 1, characterized in that: The adjustable chromatography component comprises a chromatography tube; a first baffle movably penetrates the top of the chromatography tube; a rotating block is rotatably connected to the top of the first baffle; and two groups of clamping blocks are symmetrically arranged at the bottom of the first baffle.

9. The high-performance affinity chromatography bilirubin extraction device according to claim 8, characterized in that: The bottom of the chromatography tube is rotatably connected to a second baffle; the first baffle and the second baffle are evenly provided with a plurality of liquid holes; the top of the second baffle is provided with a spoiler; the spoiler is provided with a plurality of communication holes distributed in a rectangular array.

10. The high-performance affinity chromatography bilirubin extraction device according to claim 9, characterized in that: One end of the rotating block extends to the inside of the first baffle and is transmission-connected with a gear; a group of racks that can only slide horizontally are respectively meshed and connected on both sides of the gear; each group of racks is transmission-connected with a corresponding group of clamping blocks.

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