Radioactive monodisperse particle preparation, aerosol generation and animal inhalation deposition in-situ quantification method

Abstract

The invention relates to the field of intelligent medical treatment, in particular to a radioactive monodisperse particle preparation, aerosol generation and animal inhalation deposition in-situ quantification method. Comprising the following steps: acquiring aerosol generation standard particles and radioisotopes; adding the standard particles and radioactive isotopes into a PBS solution, and carrying out an oxidation-reduction reaction to obtain a reaction solution; the reaction solution is washed and filtered through deionized water until no separable gamma activity exists, and the radioactive standard particles used for aerosol generation are obtained.The radioactive aerosol generation particles with the single particle size can be obtained and have good clinical value.

Description

Technical Field

[0001] This application relates to the field of intelligent medicine, and specifically relates to a method, device, program product and computer-readable storage medium for preparing radioactive monodisperse particles, generating aerosol and in-situ quantitative animal inhalation deposition. Background Art

[0002] The research on radioactive aerosols began in the mid-20th century and is mainly used in fields such as the nuclear industry, medical radiotherapy, and environmental radioactive pollution monitoring. In animal models, the research on the deposition distribution of radioactive aerosols is an important means to evaluate the potential hazards of inhaled radioactive substances to the human body. By simulating the human respiratory tract structure and using animals (such as mice, rabbits, monkeys, etc.) to study the deposition pattern of aerosol particles in the respiratory tract, in order to understand how aerosols with different particle sizes, shapes, and chemical properties affect their distribution in the respiratory system. The deposition distribution of aerosols in the respiratory tract may be very uneven, which depends on the aerosol particle size, shape, density, as well as the geometric structure and hydrodynamic characteristics of the respiratory tract. Among them, the aerosol particle size has a significant impact on its deposition in the respiratory tract. Smaller particles can penetrate deep into the bronchioles and alveoli, while larger particles are deposited in the upper respiratory tract. Currently, most of the existing radioactive animal inhalation exposure aerosol generators use two types of generators, namely liquid aerosol generators and electric spark + condensation devices, for quasi-monodisperse (polydisperse) aerosol generation. At the same time, the existing small animal deposition distribution quantification is all regional quantification, which cannot meet the real quantification of single-sized hydrophilic / hydrophobic aerosol particles in the respiratory tract. In addition, for radioactive aerosols, their concentration in the respiratory tract is usually low, and the detection technology needs to have high sensitivity to accurately capture the deposition distribution of these tiny particles, which poses high requirements for detection equipment and methods. Summary of the Invention

[0003] In view of the above problems, the present invention proposes a method for preparing radioactive monodisperse particles, generating aerosol and in-situ quantitative animal inhalation deposition, specifically including: Obtain standard particles for aerosol generation and radioactive isotopes; Add the standard particles and radioactive isotopes to a PBS solution to obtain a reaction solution through a redox reaction; Rinse and filter the reaction solution with deionized water until there is no separable γ activity, to obtain radioactive standard particles for aerosol generation.

[0004] The radioactive isotope includes one or more of the following: 125 I-NaI, 123 I-NaI, 131 I-NaI.

[0005] The standard particles include one or more of the following: polystyrene standard particles, polystyrene-divinylbenzene standard particles.

[0006] The PBS solution further includes chloramine-T, and the chloramine-T, standard particles, and radioactive isotope undergo a redox reaction in the PBS solution to obtain a reaction solution.

[0007] The standard particles have a single particle size, and the particle size range of the standard particles is 0.2 μm - 10 μm.

[0008] The method further includes aerosol generation: Based on the radioactive standard particles, a radioactive aerosol generating solution is prepared, and the radioactive aerosol generating solution is added to an aerosol generator to obtain monodisperse radioactive aerosol. The aerosol generator includes a polydisperse aerosol generator and an aerosol diffusion drying tube. The radioactive aerosol generating solution generates polydisperse radioactive aerosol in the polydisperse aerosol generator, and the polydisperse radioactive aerosol passes through the aerosol diffusion drying tube to obtain monodisperse radioactive aerosol; Optionally, the particle size range of the monodisperse radioactive aerosol is 0.2 μm - 10 μm; Optionally, the monodisperse radioactive aerosol is a radioactive hydrophilic aerosol or a radioactive hydrophobic aerosol; Optionally, the preparation of the radioactive aerosol generating solution is to mix the radioactive standard particles with ultrapure water to obtain the radioactive aerosol generating solution.

[0009] Optionally, the polydisperse aerosol generator includes one or more of the following: Collision three-hole polydisperse aerosol generator, German 7811 polydisperse aerosol generator, TDA-4B polydisperse aerosol generator, TDA-6C polydisperse aerosol generator, MODEL 3990-01 polydisperse aerosol generator.

[0010] The object of the present invention is a computer program product having a computer program or instruction, and the computer program or instruction is executed by a processor to implement the above-mentioned method for preparing radioactive monodisperse particles, aerosol generation, and in-situ quantitative animal inhalation deposition.

[0011] The object of the present invention is to provide a computer device, which includes a memory, a processor, and a computer program or instruction stored in the memory, and the computer program or instruction is executed by the processor to implement the above-mentioned method for preparing radioactive monodisperse particles, aerosol generation, and in-situ quantitative animal inhalation deposition.

[0012] An object of the present invention is a computer-readable storage medium, on which a computer program or instruction is stored, and the computer program or instruction is executed by a processor to implement the above-mentioned method for preparing radioactive monodisperse particles, aerosol generation, and in-situ quantitative animal inhalation deposition.

[0013] An object of the present invention is to provide a method for quantitatively determining the deposition distribution of radioactive particles in the respiratory tract, including: Obtaining monodisperse radioactive aerosol according to the above-mentioned method for preparing radioactive monodisperse particles, aerosol generation, and in-situ quantitative animal inhalation deposition; Performing tomographic scanning on an animal inhaling the monodisperse radioactive aerosol to obtain respiratory tract image data; Obtaining a particle deposition distribution map based on the respiratory tract image data.

[0014] An object of the present invention is a computer program product, on which a computer program or instruction is stored, and the computer program or instruction is executed by a processor to implement the above-mentioned method for quantitatively determining the deposition distribution of radioactive particles in the respiratory tract.

[0015] An object of the present invention is to provide a computer device, which includes a memory, a processor, and a computer program or instruction stored on the memory, and the computer program or instruction is executed by the processor to implement the above-mentioned method for quantitatively determining the deposition distribution of radioactive particles in the respiratory tract.

[0016] An object of the present invention is a computer-readable storage medium, on which a computer program or instruction is stored, and the computer program or instruction is executed by a processor to implement the above-mentioned method for quantitatively determining the deposition distribution of radioactive particles in the respiratory tract.

[0017] An object of the present invention is to provide a method for constructing a deposition distribution prediction model, including: Obtaining an atlas of deposition distribution maps and labels of radioactive particles deposited in the respiratory tract of animals with different particle sizes; Inputting the atlas of deposition distribution maps and labels into a neural network model for training to obtain a deposition distribution prediction model; Wherein, the deposition distribution map is obtained by the above-mentioned method for quantitatively determining the deposition distribution of radioactive particles in the respiratory tract.

[0018] An object of the present invention is a computer program product, on which a computer program or instruction is stored, and the computer program or instruction is executed by a processor to implement the above-mentioned method for constructing a deposition distribution prediction model.

[0019] An object of the present invention is to provide a computer device, which includes a memory, a processor, and a computer program or instruction stored on the memory, and the computer program or instruction is executed by the processor to implement the above-mentioned method for constructing a deposition distribution prediction model.

[0020] An object of the present invention is a computer-readable storage medium having a computer program or instruction stored thereon, and the computer program or instruction is executed by a processor to implement the above-mentioned method for constructing a deposition distribution prediction model.

[0021] An object of the present invention is to provide a method for predicting the deposition distribution of radioactive particles in the respiratory tract of animals, including: Obtaining a monodisperse radioactive aerosol particle size distribution map and data; Inputting the monodisperse aerosol particle size distribution map and data into the prediction model obtained by the above-mentioned method for constructing a deposition distribution prediction model to obtain the deposition distribution of particulate matter in the respiratory tract.

[0022] An object of the present invention is a computer program product having a computer program or instruction stored thereon, and the computer program or instruction is executed by a processor to implement the above-mentioned method for predicting the deposition distribution of radioactive particles in the respiratory tract of animals.

[0023] An object of the present invention is to provide a computer device, which includes a memory, a processor, and a computer program or instruction stored on the memory, and the computer program or instruction is executed by the processor to implement the above-mentioned method for predicting the deposition distribution of radioactive particles in the respiratory tract of animals.

[0024] An object of the present invention is a computer-readable storage medium having a computer program or instruction stored thereon, and the computer program or instruction is executed by a processor to implement the above-mentioned method for predicting the deposition distribution of radioactive particles in the respiratory tract of animals.

[0025] Advantages of the present invention: 1. The present invention proposes a method for preparing radioactive monodisperse particles, generating aerosol, and in-situ quantitatively depositing in animal inhalation. Different from the existing radioactive aerosol types, it uses radioactive isotope 125 I-NaI for labeling. The aerosol generated based on this radioactive isotope can display the deposition distribution of aerosol particles in the entire respiratory tract through tomographic scan image data in an animal model, which is helpful for the study of the deposition distribution of aerosol with different particle sizes.

[0026] 2. In the generation of radioactive aerosol in the present invention, hydrophilic particles and hydrophobic particles are used for preparation, specifically polystyrene and polystyrene-divinylbenzene, which helps to explore the deposition distribution law of hydrophilic particles and hydrophobic particles in the entire respiratory tract of animals. And radioactive standard particles with different particle sizes are prepared, revealing the stability of radioactive standard particles with different particle sizes during the preparation process, providing a stable precondition for generating aerosol in a single-particle-size monodisperse aerosol generator in the follow-up.

[0027] 3. The quantitative deposition distribution of the present invention is for the deposition distribution of hydrophilic or hydrophobic particles of a single particle size in the entire respiratory tract of animals, providing assistance for targeted therapy and drug delivery.

[0028] 4. A monodisperse aerosol generation method for animals is proposed. The monodispersity of this method can provide a single particle size, and the particle size has a high degree of consistency, making the experimental results more reliable and reproducible. Moreover, the monodisperse aerosol can be deposited at specific sites. By adjusting the particle size, the aerosol can be deposited at specific respiratory sites (such as the upper respiratory tract, lower respiratory tract, or alveolar region), which is suitable for studying the biological effects of specific sites. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present invention. For those skilled in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0030] Figure 1 Schematic flow diagram of a method for preparing radioactive monodisperse particles, generating aerosol, and in-situ quantification of animal inhalation deposition provided by an embodiment of the present invention; Figure 2 Schematic diagram of a system for preparing radioactive monodisperse particles, generating aerosol, and in-situ quantification of animal inhalation deposition provided by an embodiment of the present invention; Figure 3 Schematic diagram of a device for preparing radioactive monodisperse particles, generating aerosol, and in-situ quantification of animal inhalation deposition provided by an embodiment of the present invention; Figure 4 Preparation process of radioactive standard particles and aerosol generation liquid provided by an embodiment of the present invention; Figure 5 Ratio of surface radioactivity of the radioactive aerosol generation liquid after 5 hours of labeling to that at the initial labeling provided by an embodiment of the present invention; Figure 6 Animal exposure and radioactive aerosol recovery system provided by an embodiment of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0031] In order to enable those skilled in the art to better understand the solutions of the present invention, the following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention.

[0032] In some of the processes described in the specification, claims, and above-mentioned drawings of the present invention, a plurality of operations appear in a specific order. However, it should be clearly understood that these operations may not be executed in the order in which they appear herein or may be executed in parallel. The serial numbers of the operations, such as S101, S102, etc., are only used to distinguish different operations, and the serial numbers themselves do not represent any execution order. In addition, these processes may include more or fewer operations, and these operations may be executed in sequence or in parallel. It should be noted that the descriptions such as "first", "second", etc. in this article are used to distinguish different messages, devices, modules, etc., and do not represent a sequence, nor do they limit that "first" and "second" are of different types.

[0033] Figure 1 Schematic diagram of a method for preparing radioactive monodisperse particles, aerosol generation, and in-situ quantitative animal inhalation deposition provided by an embodiment of the present invention, specifically including: S101: Obtain standard particles for aerosol generation and radioactive isotopes; In one embodiment, the ratio of the standard particles to the PBS solution is 1:1.

[0034] In one embodiment, the radioactive isotope includes one or more of the following: 125 I-NaI, 123 I-NaI, 131 I-NaI; In one embodiment, the standard particles include one or more of the following: polystyrene standard particles, polystyrene-divinylbenzene standard particles.

[0035] In one embodiment, the standard particles have a single particle size, and the particle size range of the standard particles is 0.2 μm - 10 μm.

[0036] In a specific embodiment, the raw materials include polystyrene particles (Thermo Scientific, USA), polystyrene-divinylbenzene cross-linked microspheres (Thermo Scientific, USA), PBS solution (Thermo Scientific, USA), 125 I -NaI (McMaster University, Canada), PBS (Sigma-Aldrich, USA), chloramine-T (Sigma-Aldrich, USA).

[0037] In a specific embodiment, the preparation materials further include an ultrafiltration tube (Sigma-Aldrich, USA) containing a porous cellulose membrane (MWCO: 100 KD), and a porous cellulose filter membrane (Sigma-Aldrich, USA).

[0038] S102: Add the standard particles and the radioactive isotope to a PBS solution to obtain a reaction solution through a redox reaction; In one embodiment, the PBS solution further includes chloramine-T, and the chloramine-T, standard particles, and radioactive isotope undergo a redox reaction in the PBS solution to obtain a reaction solution.

[0039] In a specific embodiment, 5 mg of standard particles are added to 5 mL of PBS, and 2000 μCi 125 I-NaI and 200 μL of chloramine-T with a concentration of 4 mg / mL are added, and the reaction is carried out at 25 °C in PBS for 30 min.

[0040] S103: Rinse and filter the reaction solution with deionized water until no separable γ activity remains, to obtain radioactive standard particles for generating an aerosol.

[0041] In one embodiment, the method further includes the preparation of a radioactive aerosol generating solution. Obtain ultrapure water, and add the radioactive standard particles to the ultrapure water and mix to obtain a radioactive aerosol generating solution.

[0042] In one embodiment, no separable γ activity means that no separable γ can be detected.

[0043] In a specific embodiment, an ultrafiltration tube containing a porous cellulose membrane (MWCO: 100 KD) is used for centrifugal separation of particles to remove the excess 125 I, and then it is washed with deionized water at least 5 times until no separable γ activity remains in the filtrate solution. Then, it is mixed with ultrapure water to prepare an aerosol generating solution. The preparation processes of the radioactive standard particles and the radioactive aerosol generating solution are as Figure 4 shown.

[0044] In a specific embodiment, the generating solution is left to stand for 5 hours for measurement, and the MiniScanPRO Radio-iTLC is used to measure the stability of the radioactive calibration of polystyrene particles. For 0.2 μm - 5 μm 125 I-polystyrene and 0.2 μm - 5 μm 125 I-polystyrene-divinylbenzene, the ratio of the surface radioactivity of the particles at 5 hours after labeling to the initial moment is statistically analyzed. The radioactivity purity at 5 hours after labeling is the same as that at the initial moment, indicating that the radioactive standard particles / radioactive aerosol generating solution prepared by this method have good radioactive stability. Specifically, the results of the radioactive stability are as Figure 5 shown, for the radioactive change results of radioactive standard particles with different batches and different particle sizes.

[0045] In one embodiment, the method further includes aerosol generation: Obtain an aerosol generating liquid; Prepare a radioactive aerosol generating liquid based on the radioactive standard particles, and add the radioactive aerosol generating liquid to an aerosol generator to obtain monodisperse radioactive aerosol. The aerosol generator includes a polydisperse aerosol generator and an aerosol diffusion drying tube. The radioactive aerosol generating liquid generates polydisperse radioactive aerosol in the polydisperse aerosol generator, and the polydisperse radioactive aerosol passes through the aerosol diffusion drying tube to obtain monodisperse radioactive aerosol; In one embodiment, the particle size range of the monodisperse radioactive aerosol is 0.2 μm - 10 μm.

[0046] In one embodiment, the monodisperse radioactive aerosol is a radioactive hydrophilic aerosol or a radioactive hydrophobic aerosol.

[0047] In one embodiment, the preparation of the radioactive aerosol generating liquid is a radioactive aerosol generating liquid obtained by mixing radioactive standard particles with ultrapure water. Further, the radioactive standard particles and ultrapure water are mixed and then subjected to ultrasonic treatment to obtain the radioactive aerosol generating liquid.

[0048] In one embodiment, the polydisperse aerosol generator includes one or more of the following: Collision three-hole polydisperse aerosol generator, German 7811 polydisperse aerosol generator, TDA-4B polydisperse aerosol generator, TDA-6C polydisperse aerosol generator, MODEL 3990-01 polydisperse aerosol generator.

[0049] In one embodiment, the aerosol generating liquid is a radioactive aerosol generating liquid.

[0050] In one embodiment, the aerosol generator includes a polydisperse aerosol generator and an aerosol diffusion drying tube. The radioactive standard particles obtain polydisperse aerosol through the polydisperse aerosol generator, and the polydisperse aerosol obtains monodisperse radioactive aerosol through the aerosol diffusion drying tube.

[0051] The disclosed embodiments of the present invention also provide a computer program product or system, including a computer program, which when executed by a processor implements the above steps of preparing radioactive monodisperse particles, aerosol generation, and in-situ quantitative method of animal inhalation deposition.

[0052] Figure 2 The schematic diagram of the system for preparing radioactive monodisperse particles, aerosol generation, and in-situ quantitative animal inhalation deposition provided by the embodiments of the present invention specifically includes: Acquisition unit: Acquire standard particles for aerosol generation and radioactive isotopes; Reaction unit: Add the standard particles and radioactive isotopes to a PBS solution and obtain a reaction solution through a redox reaction; Preparation unit: Rinse and filter the reaction solution with deionized water until no separable γ activity remains, obtaining radioactive standard particles for aerosol generation.

[0053] Figure 3 Schematic diagram of the equipment for preparing radioactive monodisperse particles, generating aerosols, and in-situ quantitative animal inhalation deposition provided by the embodiments of the present invention, specifically including: A memory and a processor; the memory is used to store program instructions; the processor is used to call the program instructions, and when the program instructions are executed, any one of the above-mentioned methods for preparing radioactive monodisperse particles, generating aerosols, and in-situ quantitative animal inhalation deposition.

[0054] The disclosed embodiments of the present invention also provide a computer-readable storage medium, which stores a computer program, and when the computer program is executed by a processor, any one of the above-mentioned methods for preparing radioactive monodisperse particles, generating aerosols, and in-situ quantitative animal inhalation deposition.

[0055] The embodiments of the present invention provide a radioactive aerosol generating solution, which is a solution obtained by mixing radioactive standard particles and ultrapure water, wherein the radioactive standard particles are obtained by the above-mentioned methods for preparing radioactive monodisperse particles, generating aerosols, and in-situ quantitative animal inhalation deposition.

[0056] The embodiments of the present invention provide a method for quantitatively determining the deposition distribution of radioactive particles in the respiratory tract, including: Obtain monodisperse radioactive aerosols according to the above-mentioned methods for preparing radioactive monodisperse particles, generating aerosols, and in-situ quantitative animal inhalation deposition; Perform tomographic scanning on the animal inhaling the monodisperse radioactive aerosol to obtain respiratory tract image data; Obtain a particle deposition distribution map based on the respiratory tract image data.

[0057] In one embodiment, the imaging uses one or more of the following: SPECT, CT, SPECT-CT, MRI, X-ray.

[0058] In one embodiment, the monodisperse radioactive aerosol is a hydrophilic / hydrophobic aerosol with a single particle size. After the animal model inhales it, the whole respiratory tract image data of the animal model is obtained, and further the deposition distribution of the aerosol with a single particle size in the whole respiratory tract is obtained. The whole respiratory tract includes the alveolar region and the non-alveolar region.

[0059] In one embodiment, a radioactive isotope is used 125 125 I-NaI is used to label polystyrene standard particles based on the principle of redox reaction to construct radioactive monodisperse standard particles. Subsequently, a Collision three-hole polydisperse aerosol generator is combined with an aerosol drying tube to generate monodisperse radioactive aerosol. Then, an existing small animal breathing exposure system is combined with an exhaust gas recovery system to expose guinea pigs to inhaled radioactive aerosol. Finally, the exposed guinea pigs are subjected to high-resolution SPECT / CT detection to quantitatively determine the deposition distribution.

[0060] In one embodiment, the aerosol generator further includes an air compressor, a cascade filter, a valve, and a flow meter. Non-clean gas is obtained through the air compressor, and the non-clean gas is filtered through the cascade filter to obtain clean gas. The clean gas is controlled by the valve to enter the polydisperse aerosol generator. The flow rate of the gas is measured by the flow meter. Standard particles and aerosol generation liquid are added to the polydisperse aerosol generator. When the gas flow rate reaches the generation condition, the valve is opened to generate polydisperse aerosol to obtain polydisperse aerosol, and the polydisperse aerosol is dried through an aerosol diffusion drying tube to obtain monodisperse aerosol.

[0061] In a specific embodiment, the process of obtaining radioactive monodisperse aerosol from a radioactive standard particle aerosol generation liquid is as follows: Experimental materials: polystyrene particles (Thermo Scientific, USA), polystyrene-divinylbenzene cross-linked microspheres (Thermo Scientific, USA), ultrapure water, aerosol diffusion dryer (HRH-DRY2, Huironghe Technology Co., Ltd.), Collision three-hole polydisperse aerosol generator (HRH-WAG3, Huironghe Technology Co., Ltd.), air compressor (Huironghe Technology Co., Ltd.), cascade filter (Huironghe Technology Co., Ltd.), valve (Huironghe Technology Co., Ltd.), flow meter (Huironghe Technology Co., Ltd.).

[0062] Radioactive polystyrene particles and radioactive polystyrene-divinylbenzene particle aerosol generation liquid are added to the Collision three-hole polydisperse aerosol generator, and generation and drying are carried out in combination with the Huironghe HRH-DRY2 aerosol diffusion drying tube. The aerosol particle size spectrum is measured using a TSI3321 aerodynamic particle sizer. The results show that the aerosol particle size spectrum shows good concentration at aerosol particle sizes of 0.7 μm and 3 μm, with GSD < 1.2, meeting the monodisperse condition.

[0063] In a specific embodiment, the process of exposing animals to a radioactive aerosol environment for inhalation and recovering the remaining aerosol is as follows: Experimental materials: nose and mouth exposure tower (Silicon Motion Technology Corporation), TSI 3321 Aerodynamic Particle Sizer, TSI Scanning Mobility Particle Sizer (TSI 3756, TSI 3081A, TSI 3082, TSI 3088), cascade filter (Silicon Motion Technology Corporation), flowmeter (Silicon Motion Technology Corporation), valve (Silicon Motion Technology Corporation), air pump (Silicon Motion Technology Corporation), small animal SPECT / CT (Burker, USA).

[0064] First, prepare monodisperse aerosol from radioactive standard particles and introduce it into the nose and mouth exposure tower to construct a stable gas environment (monitor the aerosol particle size spectrum using the TSI 3321 Aerodynamic Particle Sizer and the TSI Scanning Mobility Particle Sizer; then place guinea pigs in the respiratory exposure chamber for nose and mouth exposure breathing for 2 hours. After the exposure, anesthetize the guinea pigs with 5 mL of 0.25% sodium pentobarbital and use SPECT / CT to quantitatively analyze the particle deposition distribution in the guinea pigs. Specifically, as shown in Figure 6 After the aerosol is transported to the exposure tower, the animals inhale through the exposure tower, and the remaining radioactive aerosol is recovered successively through the cascade filter, flowmeter + valve, and air pump.

[0065] In a specific embodiment, the SPECT / CT quantitative results show that the constructed system can quantitatively analyze the deposition distribution of particles with different particle sizes in the respiratory tract with high resolution, and there are significant differences in the deposition distribution of 0.7-micron particles and 3-micron particles in the respiratory tract.

[0066] The disclosed embodiment of the present invention also provides a computer program product or system, including a computer program, which when executed by a processor implements the steps of the above-mentioned method for detecting the respiratory tract particle deposition distribution based on radioactive particles.

[0067] The detection system for the deposition distribution of radioactive particles in the respiratory tract provided by the embodiment of the present invention includes: Particle unit: Obtain radioactive standard particles according to the above-mentioned method for preparing radioactive monodisperse particles, generating aerosol, and in-situ quantitative analysis of animal inhalation deposition; Aerosol unit: Convert the radioactive standard particles into radioactive aerosol through an aerosol generator; Imaging unit: Take images of the inhaled radioactive aerosol to obtain respiratory tract image data; Deposition distribution unit: Obtain the particle deposition distribution result based on the respiratory tract image data.

[0068] The object of the present invention is to provide a computer device, which includes a memory, a processor, and a computer program or instruction stored on the memory, and the computer program or instruction is executed by the processor to implement the above-mentioned method for detecting the deposition distribution of radioactive particles in the respiratory tract.

[0069] The object of the present invention is a computer-readable storage medium, on which a computer program or instruction is stored, and the computer program or instruction is executed by the processor to implement the above-mentioned method for detecting the deposition distribution of respiratory tract particles based on radioactive particles.

[0070] An embodiment of the present invention provides a method for constructing a deposition distribution prediction model, including: Obtaining an atlas and labels of the deposition distribution of radioactive particles with different particle sizes in the respiratory tract of animals; Inputting the deposition distribution atlas and labels into a neural network model for training to obtain a deposition distribution prediction model; Wherein, the deposition distribution map is obtained by the above-mentioned method for quantitatively detecting the deposition distribution of radioactive particles in the respiratory tract.

[0071] An embodiment of the present invention provides a method for predicting the deposition distribution of radioactive particles in the respiratory tract of animals, including: Obtaining a monodisperse radioactive aerosol particle size distribution map and data; Inputting the monodisperse aerosol particle size distribution map and data into the prediction model obtained by the above-mentioned method for constructing a deposition distribution prediction model to obtain the deposition distribution of particulate matter in the respiratory tract.

[0072] The verification results of this verification embodiment show that assigning fixed weights to indications can improve the performance of this method compared to the default settings. Those skilled in the art can clearly understand that for the convenience and brevity of description, the specific working processes of the systems, devices, and units described above can refer to the corresponding processes in the foregoing method embodiments and will not be elaborated herein. In several embodiments provided by this application, it should be understood that the disclosed systems, devices, and methods can be implemented in other ways. For example, the device embodiments described above are merely illustrative. For example, the division of the units is only a logical function division, and there may be other division methods in actual implementation. For example, multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the displayed or discussed couplings or direct couplings or communication connections to each other can be through some interfaces, and the indirect couplings or communication connections of the devices or units can be in electrical, mechanical, or other forms. The units described as separate components may or may not be physically separated, and the components displayed as units may or may not be physical units, that is, they can be located in one place or distributed to multiple network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of this embodiment. In addition, in each embodiment of the present invention, the functional units can be integrated in a processing unit, or each unit can exist physically alone, or two or more units can be integrated in one unit. The above-mentioned integrated units can be implemented in the form of hardware or in the form of software functional units. Those of ordinary skill in the art can understand that all or part of the steps in the various methods of the above embodiments can be completed by instructing relevant hardware through a program, and the program can be stored in a computer-readable storage medium. The storage medium can include: read-only memory (ROM, Read Only Memory), random access memory (RAM, Random Access Memory), magnetic disk, or optical disc, etc.

[0073] Those of ordinary skill in the art can understand that all or part of the steps in implementing the methods of the above embodiments can be completed by instructing relevant hardware through a program, and the program can be stored in a computer-readable storage medium. The above-mentioned medium storage can be read-only memory, magnetic disk, or optical disc, etc.

[0074] The above has introduced in detail a computer device provided by the present invention. For those of ordinary skill in the art, according to the idea of the embodiments of the present invention, there will be changes in the specific implementation manners and application scopes. In summary, the content of this specification should not be construed as a limitation to the present invention.

Claims

1. A method for preparing radioactive monodisperse particles, generating aerosols and in-situ quantitative deposition by animal inhalation, characterized in that: include: Obtain standard particles and radioisotopes for aerosol generation; Adding the standard particles and radioactive isotopes into a PBS solution to obtain a reaction solution through an oxidation-reduction reaction; The reaction solution was filtered by washing with deionized water until there was no isolatable gamma activity, thereby obtaining radioactive standard particles for aerosol generation.

2. The in-situ quantitative method for preparation of radioactive monodisperse particles, aerosol generation and animal inhalation deposition according to claim 1, characterized in that: The radioactive isotopes include one or more of the following: 125 I-NaI, 123 I-NaI, 131 I-NaI; Optionally, the standard particles include one or more of the following: polystyrene standard particles, polystyrene-divinylbenzene standard particles; Optionally, the PBS solution further includes chloramine-T, and the chloramine-T, standard particles, and radioactive isotopes are subjected to a redox reaction in the PBS solution to obtain a reaction solution; Optionally, the particle size of the standard particles is a single particle size, and the particle size range of the standard particles is 0.2 μm-10 μm.

3. The in-situ quantitative method for preparing radioactive monodisperse particles, generating aerosols and inhaling deposition by animals according to claim 1, characterized in that: The method further comprises aerosol generation: preparing a radioactive aerosol generating liquid based on the radioactive standard particles, adding the radioactive aerosol generating liquid into an aerosol generator to obtain a monodisperse radioactive aerosol, wherein the aerosol generator comprises a polydisperse aerosol generator and an aerosol diffusion drying tube, the radioactive aerosol generating liquid generates a polydisperse radioactive aerosol in the polydisperse aerosol generator, and the polydisperse radioactive aerosol passes through the aerosol diffusion drying tube to obtain a monodisperse radioactive aerosol; Optionally, the particle size of the monodisperse radioactive aerosol ranges from 0.2 μm to 10 μm; Optionally, the monodisperse radioactive aerosol is a radioactive hydrophilic aerosol or a radioactive hydrophobic aerosol; Optionally, the polydisperse aerosol generator includes one or more of the following: Collision three-hole polydisperse aerosol generator, German 7811 polydisperse aerosol generator, TDA-4B polydisperse aerosol generator, TDA-6C polydisperse aerosol generator, MODEL 3990-01 polydisperse aerosol generator.

4. The method for preparing radioactive monodisperse particles, generating aerosols and in-situ quantitative deposition by animal inhalation according to claim 1, characterized in that: The preparation method comprises mixing radioactive standard particles with ultrapure water to obtain radioactive aerosol generating liquid.

5. A quantitative method for the deposition and distribution of radioactive particles in the respiratory tract, characterized in that: include: The monodisperse radioactive aerosol is obtained according to the in-situ quantitative method for preparing radioactive monodisperse particles, generating aerosols and inhaling deposition by animals as described in claim 3; Tomographically scanning the animals that inhale the monodisperse radioactive aerosol to obtain respiratory imaging data; A particle deposition distribution map is obtained based on the respiratory tract image data.

6. A method for constructing a sedimentation distribution prediction model, characterized in that: include: Obtain deposition distribution atlases and labels of radioactive particles of different sizes deposited in the respiratory tract of animals; Inputting the deposition distribution atlas and labels into a neural network model for training to obtain a deposition distribution prediction model; Wherein, the deposition distribution diagram is obtained by the quantitative method for the deposition distribution of radioactive particles in the respiratory tract as described in claim 5.

7. A method for predicting the deposition distribution of radioactive particles in the respiratory tract of an animal, characterized in that: include: Obtain monodisperse radioactive aerosol particle size distribution map and data; The monodisperse aerosol particle size distribution diagram and data are input into the prediction model obtained by the method for constructing the deposition distribution prediction model described in claim 6 to obtain the deposition distribution of particles in the respiratory tract.

8. A computer program product having a computer program or instructions thereon, characterized in that: The computer program or instructions are executed by the processor to implement the in situ quantitative method for preparing radioactive monodisperse particles, generating aerosols and depositing by animal inhalation as described in any one of claims 1 to 3, or to implement the quantitative method for the deposition and distribution of radioactive particles in the respiratory tract as described in claim 5, or to implement the method for constructing a deposition distribution prediction model as described in claim 6, or to implement the method for predicting the deposition and distribution of radioactive particles in the respiratory tract of animals as described in claim 7.

9. A computer device comprising a memory, a processor and a computer program or instruction stored in the memory, characterized in that: The computer program or instructions are executed by the processor to implement the in situ quantitative method for preparing radioactive monodisperse particles, generating aerosols and depositing by animal inhalation as described in any one of claims 1 to 3, or to implement the quantitative method for the deposition and distribution of radioactive particles in the respiratory tract as described in claim 5, or to implement the method for constructing a deposition distribution prediction model as described in claim 6, or to implement the method for predicting the deposition and distribution of radioactive particles in the respiratory tract of animals as described in claim 7.

10. A computer-readable storage medium having a computer program or instruction stored thereon, characterized in that: The computer program or instructions are executed by the processor to implement the in situ quantitative method for preparing radioactive monodisperse particles, generating aerosols and depositing by animal inhalation as described in any one of claims 1 to 3, or to implement the quantitative method for the deposition and distribution of radioactive particles in the respiratory tract as described in claim 5, or to implement the method for constructing a deposition distribution prediction model as described in claim 6, or to implement the method for predicting the deposition and distribution of radioactive particles in the respiratory tract of animals as described in claim 7.

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