Pressure type microfluidic chip and detection device thereof

Abstract

The application relates to the technical field of nucleic acid detection, in particular to a pressure type micro-fluidic chip and a detection device thereof. The micro-fluidic chip comprises a chip body, a sample adding hole, a detection pool, a plurality of reaction pools, a plurality of reagent pools and a plurality of flow channels are arranged on the chip body, the plurality of reaction pools are sequentially communicated, the plurality of reaction pools are located between the sample adding hole and the detection pool, the reagent pool is correspondingly arranged around the reaction pool, the flow channel communicates the openings between the adjacent reaction pools, and the flow channel also communicates the opening of the reagent pool and the opening of the corresponding reaction pool. The solution in the reaction pool and the reagent pool is extruded by a pressure hammer, so that all the solutions in the reaction pool flow through the flow channel. The application has the effect of reducing the solution residue in the reaction pool.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of nucleic acid detection, and in particular to a pressure type microfluidic chip and a detection device thereof. BACKGROUND

[0002] A microfluidic chip, also known as a microfluidic chip, is a technology that integrates small-scale liquid fluid processes on a small chip. Such chips are usually made of glass or plastic, with microchannels and chambers engraved on them, which can precisely control the flow and mixing of liquids in a small space. Microfluidic chips are widely used in biomedicine, chemical analysis, environmental monitoring and other fields, because they can achieve high efficiency and high sensitivity experiments while reducing the amount of reagents and samples required.

[0003] At present, one patent with publication number CN111607506B discloses a thin film type nucleic acid amplification chip body and a preparation and application method thereof. The thin film type nucleic acid amplification chip body includes a plurality of reaction chambers connected by channels; the reaction chamber includes a lysis chamber, a purification chamber, a pre-amplification chamber and an amplification chamber; the lysis chamber is provided with a sample inlet and is in communication with the purification chamber; the purification chamber is respectively communicated with a first liquid storage chamber preloaded with nucleic acid purification liquid and a second liquid storage chamber preloaded with nucleic acid eluent; the purification chamber is pre-packaged with silica-coated magnetic beads and is in communication with the pre-amplification chamber; the pre-amplification chamber is communicated with a third liquid storage chamber preloaded with nucleic acid amplification diluent and is in communication with the amplification chamber.

[0004] For the related technology in the above, the flow of the solution between the channels relies on the gravity of the solution itself, so the solution usually flows into the top side of the reaction chamber and flows out from the bottom side. However, the microfluidic chip is small and the channels are thin, and a small amount of solution at the bottom of the reaction chamber is difficult to flow out through the channel relying on gravity, so the solution in the reaction chamber is prone to residual. SUMMARY

[0005] In order to reduce the residual of the solution in each reaction chamber, improve the solution volume accuracy, speed up the liquid flow, increase the sealing effect to prevent pollution, and reduce the production cost of the chip body, the present application provides a pressure type microfluidic chip and a detection device thereof.

[0006] In the first aspect, the pressure type microfluidic chip provided by the present application adopts the following technical scheme:

[0007] The pressure type micro-fluidic chip comprises a chip body, the inside of the chip body has a cavity, and a sample adding hole, a detection pool, a plurality of reaction pools, a plurality of reagent pools and a plurality of flow channels are arranged on the chip body; the plurality of reaction pools comprise a sample lysis pool, a magnetic bead reaction pool and an amplification mixing pool which are sequentially communicated, the sample adding hole is communicated with the sample lysis pool, the detection pool is communicated with the amplification mixing pool, the bottom of the magnetic bead reaction pool is provided with a magnetic bead, one side of the opening of the magnetic bead reaction pool is further provided with a liquid discharge groove, and the liquid discharge groove is communicated with the cavity in the inside of the chip body; the plurality of reagent pools comprise a washing liquid pool, an eluent pool, an amplification liquid pool and a primer probe pool, the washing liquid pool and the eluent pool are respectively communicated with the magnetic bead reaction pool, and the amplification liquid pool and the primer probe pool are respectively communicated with the amplification mixing pool; the plurality of flow channels are used for communicating the openings between adjacent reaction pools, and are also used for communicating the openings of the reagent pools with the openings of the corresponding reaction pools.

[0008] By adopting the technical scheme, in the experiment process, the sample solution is injected into the sample lysis pool through the sample adding hole, after the sample is lysed in the sample lysis pool, the detection equipment uses the pressure hammer to extrude the solution in the sample lysis pool, the pressure hammer above the lysis pool extrudes all the solution in the lysis pool into the magnetic bead reaction pool through the flow channel, after the magnetic bead is adsorbed, the pressure hammer is used to extrude all the solution in the washing liquid pool into the magnetic bead reaction pool through the flow channel, after washing, the magnetic bead is adsorbed by using the magnet, and then the pressure hammer is used to extrude all the solution in the magnetic bead reaction pool into the cavity in the inside of the chip body through the liquid discharge groove.

[0009] Then the pressure hammer above the magnetic bead reaction pool is reset, the pressure hammer above the eluent pool extrudes all the solution in the magnetic bead reaction pool into the amplification mixing pool through the flow channel, at this time, the pressure hammers above the amplification liquid pool and the primer probe pool respectively extrude all the solution in the amplification liquid pool and the primer probe pool into the amplification mixing pool through the flow channel, after amplification is completed, the pressure hammer above the amplification mixing pool is used to extrude all the solution in the amplification mixing pool into the detection pool through the flow channel.

[0010] The solution is extruded by using the pressure hammer, so that all the solution in the reaction pool flows through the flow channel, and the solution residue in the reaction pool is reduced, and when the pressure hammer extrudes the solution in the reaction pool, the solution can flow more quickly.

[0011] Optionally, the bottoms of the reaction pool and the reagent pool are spherical, and the inner concave sides are directed to the opening direction of the reaction pool.

[0012] By adopting the technical scheme, the spherical reaction pool and the reagent pool are more easily extruded by the pressure hammer, so that the reaction pool and the reagent pool can more smoothly discharge the solution.

[0013] Optionally, the inner wall of the reaction pool and the reagent pool is provided with a flow guide groove, and one side of the flow guide groove is communicated with the flow channel on the side of the flowing direction.

[0014] By adopting the above technical scheme, the flow guide groove is provided to facilitate the solution in the reaction pool and the reagent pool to flow into the flow channel along the flow guide groove under the extrusion of the pressure hammer, thereby reducing the solution residue in the reaction pool and the reagent pool.

[0015] Optionally, a plurality of flow guide grooves are provided, and the plurality of flow guide grooves are communicated with the flow channel on the side of the flowing direction.

[0016] By adopting the above technical scheme, the plurality of flow guide grooves are provided to further enhance the flowability of the solution in the reaction pool and the reagent pool to flow into the flow channel along the flow guide groove under pressure, thereby further reducing the solution residue in the reaction pool and the reagent pool.

[0017] Optionally, the side of the same flow guide groove away from the flow channel is located on the same contour surface.

[0018] By adopting the above technical scheme, the reaction pool and the reagent pool located on each height layer can flow towards the flow channel under the extrusion of the pressure hammer.

[0019] Optionally, the reaction pool further comprises a quantitative pool, the quantitative pool is located between the magnetic bead reaction pool and the amplification mixing pool, one side of the quantitative pool is communicated with the magnetic bead reaction pool, and the other side is communicated with the amplification mixing pool; the reagent pool further comprises a residual liquid pool, and the residual liquid pool is communicated with the quantitative pool.

[0020] By adopting the above technical scheme, the solution in the magnetic bead reaction pool is transported to the quantitative pool, and the overflowed solution flows into the residual liquid pool, so that the solution transported to the amplification mixing pool in the quantitative pool is a certain amount, and the experimental data is more accurate.

[0021] Optionally, the detection pool comprises a first detection pool and a second detection pool, and the amplification mixing pool, the first detection pool and the second detection pool are communicated in sequence.

[0022] By adopting the above technical scheme, different environmental temperature solutions are placed in the first detection pool and the second detection pool, which facilitates the PCR detection of the detection personnel.

[0023] Optionally, the washing liquid pool comprises a first washing liquid pool and a second washing liquid pool, and the first washing liquid pool and the second washing liquid pool are respectively communicated with the magnetic bead reaction pool.

[0024] By adopting the technical scheme, the first washing liquid pool and the second washing liquid pool are used to enable the sample adsorbed by the magnetic beads in the magnetic bead reaction pool to be washed twice when the reaction is carried out in the magnetic bead reaction pool.

[0025] In a second aspect, the detection device provided by the application adopts the following technical scheme:

[0026] The detection device comprises the pressure type microfluidic chip, a lower clamping plate, a magnet, an electric heating element, and an upper clamping plate.

[0027] By adopting the technical scheme, in the process of detecting the nucleic acid, the chip body is placed in the limiting groove, and then the sample solution is added into the sample lysis pool through the sample adding hole.

[0028] After the sample is lysed, the driving element drives the pressure hammer located on the upper side of the sample lysis pool to extrude the solution in the sample lysis pool, and at the same time, the driving element opens the pressure valve between the sample lysis pool and the magnetic bead reaction pool, so that the solution in the sample lysis pool is completely transferred into the magnetic bead reaction pool, and then the driving element closes the pressure valve between the sample lysis pool and the magnetic bead reaction pool.

[0029] After the nucleic acid in the solution in the magnetic bead reaction pool is adsorbed by the magnetic beads, the driving element drives the pressure hammer located on the upper side of the washing liquid pool to extrude the washing liquid pool, and at the same time, the driving element opens the pressure valve between the washing liquid pool and the magnetic bead reaction pool, so that the solution in the washing liquid pool is completely transferred into the magnetic bead reaction pool, and then the driving element closes the pressure valve between the washing liquid pool and the magnetic bead reaction pool.

[0030] After the washing is completed, the magnet is close to the bottom of the magnetic bead reaction pool, the magnetic beads are adsorbed by the magnet, and then the driving element drives the pressure hammer located on the upper side of the magnetic bead reaction pool to extrude the solution in the magnetic bead reaction pool, and at the same time, the driving element opens the pressure valve above the liquid discharge groove, so that the pressure hammer completely discharges the solution in the magnetic bead reaction pool through the liquid discharge groove, and then the driving element closes the pressure valve above the liquid discharge groove, and at the same time, the driving element drives the pressure hammer located on the upper side of the magnetic bead reaction pool to reset.

[0031] Then the magnet is moved away from the bottom of the magnetic bead reaction pool, the driving member drives the pressure hammer located on the upper side of the eluent pool to extrude the solution in the eluent pool, and at the same time the driving member opens the pressure valve between the eluent pool and the magnetic bead reaction pool, so that the pressure hammer transfers all the solution in the eluent pool to the magnetic bead reaction pool, and then the driving member closes the pressure valve between the eluent pool and the magnetic bead reaction pool.

[0032] After the nucleic acid is separated from the magnetic bead, the driving member drives the pressure hammer located on the upper side of the magnetic bead reaction pool to extrude the magnetic bead reaction pool, and at the same time the driving member opens the pressure valve between the magnetic bead reaction pool and the amplification mixing pool, so that the solution in the magnetic bead reaction pool is completely transferred to the amplification mixing pool, and then the driving member closes the pressure valve between the magnetic bead reaction pool and the amplification mixing pool.

[0033] Then the driving member drives the pressure hammer located on the upper side of the amplification liquid pool to extrude the amplification liquid pool, and at the same time the driving member opens the pressure valve between the amplification liquid pool and the amplification mixing pool, so that the solution in the amplification liquid pool is completely transferred to the amplification mixing pool, and then the driving member closes the pressure valve between the amplification liquid pool and the amplification mixing pool.

[0034] Next, the driving member drives the pressure hammer located on the upper side of the primer probe pool to extrude the primer probe pool, and at the same time the driving member opens the pressure valve between the primer probe pool and the amplification mixing pool, so that the solution in the primer probe pool is completely transferred to the amplification mixing pool, and then the driving member closes the pressure valve between the primer probe pool and the amplification mixing pool.

[0035] After the nucleic acid is amplified, the driving member drives the pressure hammer located on the upper side of the amplification liquid pool to extrude the amplification mixing pool, and at the same time the driving member opens the pressure valve between the amplification mixing pool and the detection pool, so that the solution in the amplification liquid pool is completely transferred to the detection pool, and then the driving member closes the pressure valve between the amplification mixing pool and the detection pool.

[0036] The solution is extruded by the pressure hammer, so that all the solutions in the reaction pool flow through the flow channel, which accelerates the flow speed of the solution and makes the experimental process more rapid.

[0037] Optionally, the lower clamping plate is further provided with an ultrasonic device, and the ultrasonic device is located at the bottom of the sample lysis pool.

[0038] By adopting the above technical scheme, the ultrasonic device is arranged to facilitate the solution in the sample lysis pool.

[0039] In summary, the present application has at least one of the following beneficial technical effects:

[0040] 1. The chip body is provided with a drainage groove, so that the internal space of the chip body can be used to contain the washing liquid, the space for containing the washing liquid is not additionally arranged on the chip body, the containing space is larger, the structure is simpler, the area of the chip body can be reduced, and the production cost is reduced.

[0041] 2. The chip body, the reaction pool, the reagent pool and the flow channel are matched, the solution is extruded by the pressure hammer, all the solutions in the reaction pool flow through the flow channel, the solution residue in the reaction pool is reduced, and the solution can flow more quickly when the pressure hammer extrudes the solution in the reaction pool.

[0042] 3. The drainage groove is arranged, so that the solution in the reaction pool and the reagent pool can flow into the flow channel along the drainage groove under the extrusion of the pressure hammer, and the solution residue in the reaction pool and the reagent pool is reduced.

[0043] 4. The quantitative pool and the excess liquid pool are matched, so that the solution in the magnetic bead reaction pool is transported to the quantitative pool, the overflow solution flows into the excess liquid pool, the solution transported to the amplification mixing pool in the quantitative pool is a certain amount, and the experimental data is more accurate. BRIEF DESCRIPTION OF DRAWINGS

[0044] Figure 1 FIG. 1 is a schematic diagram of the overall structure of the pressure type microfluidic chip in Embodiment 1 of the present application.

[0045] Figure 2 FIG. 2 is a schematic diagram of the top view structure of the pressure type microfluidic chip in Embodiment 1 of the present application.

[0046] Figure 3 FIG. 3 is a schematic diagram of the overall structure of the detection device in Embodiment 1 of the present application.

[0047] Figure 4 FIG. 4 is a schematic diagram of the sectional structure of the lower clamping plate in Embodiment 1 of the present application.

[0048] Figure 5 FIG. 5 is a schematic diagram of the overall structure of the detection device in Embodiment 2 of the present application.

[0049] Figure 6 FIG. 6 is a schematic diagram of the overall structure of the limiting assembly in Embodiment 2 of the present application.

[0050] Figure 7 FIG. 7 is a schematic diagram of the sectional structure of the limiting assembly in Embodiment 2 of the present application.

[0051] Figure 8 FIG. 8 is a schematic diagram of the sectional structure of the mounting seat in Embodiment 2 of the present application.

[0052] Figure labeling: 1. Chip body; 2. Sample loading well; 3. Detection cell; 31. First detection cell; 32. Second detection cell; 4. Reaction cell; 41. Sample lysis cell; 42. Magnetic bead reaction cell; 43. Quantitative cell; 44. Amplification mixing cell; 5. Drainage tank; 6. Reagent tank; 61. Washing solution tank; 611. First washing solution tank; 612. Second washing solution tank; 62. Elution solution tank; 63. Residual solution tank; 64. Amplification solution tank; 65. Primer / probe tank; 7. Flow channel; 8. Drainage channel; 10. Lower clamp; 101. Limiting groove; 102. Magnet; 103. Heating element; 104. Ultrasonic device; 20. Upper clamp; 201. Pressure hammer; 202. Pressure valve; 203. Drive element; 30. Safety device. 40. Mounting base; 40. Limiting component; 401. Mounting body; 4011. Mounting cavity; 4012. Mounting support rod; 402. Mounting hole; 403. Abutting component; 4031. Limiting block; 4032. Sliding hole; 4033. Guide surface; 4034. First spring; 404. Manual drive component; 4041. Drive column; 4042. Drive hole; 4043. Second spring; 405. Converter component; 4051. Intermediate rod; 4052. First elongated hole; 4053. First column; 4054. Second elongated hole; 4055. Second column; 50. Pop-out component; 501. Pop-out plate; 502. Pop-out groove; 503. Pop-out spring; 60. Mounting component; 601. Mounting bolt; 602. Mounting elongated hole; Detailed Implementation

[0053] The following is in conjunction with the appendix Figures 1-8 This application will be described in further detail.

[0054] Example 1: This application discloses a pressure-type microfluidic chip.

[0055] Reference Figure 1 and Figure 2 A pressure-based microfluidic chip includes a chip body 1, multiple reaction cells 4, multiple reagent cells 6, and a flow channel 7. The chip body 1 has a sample dispensing port 2 and a detection cell 3. The multiple reaction cells 4 are located between the sample dispensing port 2 and the detection cell 3. The reagent cells 6 are arranged around the corresponding reaction cells 4. The flow channel 7 is formed on the chip body 1, connecting the openings between adjacent reaction cells 4, and also connecting the openings of the reagent cells 6 with the openings of the corresponding reaction cells 4.

[0056] In this embodiment, the bottoms of both reaction tank 4 and reagent tank 6 are spherical, with the concave side facing the opening of reaction tank 4. In other embodiments, the bottoms of reaction tank 4 and reagent tank 6 can also be configured as inverted triangles or inverted trapezoids. The spherical shape of reaction tank 4 and reagent tank 6 makes them more easily compressed by pressure hammer 201, allowing the solution to be discharged from reaction tank 4 and reagent tank 6 more smoothly.

[0057] With reference to Figure 1 and Figure 2 , the reaction pool 4 includes a sample lysing pool 41, a magnetic bead reaction pool 42 and an amplification mixing pool 44 connected in sequence, the sample lysing pool 41, the magnetic bead reaction pool 42 and the amplification mixing pool 44 are arranged on the chip body 1, the sample addition hole 2 is communicated with the sample lysing pool 41, the detection pool 3 is communicated with the amplification mixing pool 44, and the bottom of the magnetic bead reaction pool 42 is provided with magnetic beads. The chip body 1 is provided with a cavity, and the side of the opening of the magnetic bead reaction pool 42 is further provided with a liquid discharge groove 5, which communicates the magnetic bead reaction pool 42 to the cavity inside the chip body 1. In this embodiment, the liquid discharge groove 5 is arranged to make full use of the internal space of the chip body 1 to accommodate the washing liquid, the accommodation space is larger, the structure is simpler, the space for accommodating the washing liquid is not additionally arranged on the chip body 1, the structure of the chip body 1 is simplified, the area of the chip body 1 is reduced, and the production cost is reduced.

[0058] With reference to Figure 1 and Figure 2 , the reagent pool 6 includes a washing liquid pool 61, an eluent pool 62, an amplification liquid pool 64 and a primer probe pool 65, the washing liquid pool 61 and the eluent pool 62 are respectively communicated with the magnetic bead reaction pool 42, and the amplification liquid pool 64 and the primer probe pool 65 are respectively communicated with the amplification mixing pool 44.

[0059] The sample solution added from the sample addition hole 2 is reacted by cooperation of the sample lysing pool 41, the magnetic bead reaction pool 42, the amplification mixing pool 44, the washing liquid pool 61, the eluent pool 62, the amplification liquid pool 64 and the primer probe pool 65, and finally transferred to the detection pool 3 for observation.

[0060] With reference to Figure 1 and Figure 2 , the washing liquid pool 61 includes a first washing liquid pool 611 and a second washing liquid pool 612, and the first washing liquid pool 611 and the second washing liquid pool 612 are respectively communicated with the magnetic bead reaction pool 42.

[0061] In this embodiment, the first washing liquid pool 611 and the second washing liquid pool 612 are cooperated to enable the sample adsorbed by the magnetic beads in the magnetic bead reaction pool 42 to be washed twice during the reaction in the magnetic bead reaction pool 42, and the first washing liquid pool 611 and the second washing liquid pool 612 can accommodate washing liquids with different properties or functions according to needs, so that the washing is more thorough. Moreover, since the magnetic bead reaction pool 42 is communicated to the cavity inside the chip body 1 through the liquid discharge groove 5, more washing liquid generated by multiple washing can be processed.

[0062] With reference to Figure 1 and Figure 2The reaction pool 4 further comprises a dosing pool 43, which is located between the magnetic bead reaction pool 42 and the amplification mixing pool 44, and is in communication with the magnetic bead reaction pool 42 on one side and the amplification mixing pool 44 on the other side. The reagent pool 6 further comprises a residual liquid pool 63, which is in communication with the dosing pool 43.

[0063] The cooperation of the dosing pool 43 and the residual liquid pool 63 enables the solution in the magnetic bead reaction pool 42 to flow into the dosing pool 43, and the overflow solution to flow into the residual liquid pool 63, so that the solution in the dosing pool 43 is dosed to the amplification mixing pool 44, and the solution volume control is more accurate, and the experimental data is more accurate.

[0064] Referring to Figure 1 and Figure 2 , the detection pool 3 further comprises a first detection pool 31 and a second detection pool 32, and the amplification mixing pool 44, the first detection pool 31 and the second detection pool 32 are in communication in sequence. The solutions with different environmental temperatures are placed in the first detection pool 31 and the second detection pool 32, which greatly improves the detection efficiency of the detection personnel.

[0065] Referring to Figure 1 and Figure 2 , the inner walls of the reaction pool 4 and the reagent pool 6 are provided with drainage grooves 8. The drainage grooves 8 are arranged to facilitate the solution in the reaction pool 4 and the reagent pool 6 to flow into the flow-through groove 7 along the drainage grooves 8 under the extrusion of the pressure hammer 201, thereby reducing the solution residue in the reaction pool 4 and the reagent pool 6.

[0066] Referring to Figure 1 and Figure 2 , the drainage grooves 8 are provided in multiple, and the multiple drainage grooves 8 are in communication with the flow-through grooves 7 on the side of the flow direction. In the embodiment, the drainage grooves 8 are provided in three, and in other embodiments, two, four or more drainage grooves 8 can be provided according to the size of the reaction pool 4 and the reagent pool 6. The side of all the drainage grooves 8 is in communication with the flow-through grooves 7 on the side of the flow direction, and the side of the same drainage groove 8 away from the flow-through groove 7 is located on the same level surface.

[0067] The arrangement of the multiple drainage grooves 8 further enhances the flowability of the solution in the reaction pool 4 and the reagent pool 6 along the drainage grooves 8 to the corresponding flow-through grooves 7 when the solution is under pressure, thereby further reducing the solution residue in the reaction pool 4 and the reagent pool 6. Meanwhile, the side of the drainage grooves 8 away from the flow-through grooves 7 is located on the same level surface, so that the reaction pool 4 and the reagent pool 6 located on each height layer can flow towards the flow-through grooves 7 under the extrusion of the pressure hammer 201.

[0068] In this embodiment, the pressure type microfluidic chip needs to provide pressure for the pressure hammer 201 of the corresponding detection equipment to realize the flow of the solution in the chip body 1. In order to prevent the solution from overflowing to the upper side of the chip body 1 under the action of the pressure hammer 201, a separation film is covered on the upper side of the chip body 1, and the separation film is bonded to the surface of the chip body 1, so that the solution can only flow along the corresponding flow channel 7.

[0069] The implementation principle of the pressure type microfluidic chip in the embodiment of the application is as follows: in the experiment, the sample solution is injected into the sample lysis pool 41 through the sample adding hole 2, after the sample is lysed in the sample lysis pool 41, the detection equipment uses the pressure hammer 201 to press the solution in the sample lysis pool 41, and the solution in the sample lysis pool 41 is pressed through the flow channel 7 to the magnetic bead reaction pool 42, after the magnetic beads are adsorbed, the pressure hammer 201 is used to press the solution in the first washing liquid pool 611 through the flow channel 7 to the magnetic bead reaction pool 42, after washing, the magnetic beads are adsorbed by the magnet 102, and then the pressure hammer 201 is used again to press the solution in the magnetic bead reaction pool 42 through the liquid discharge groove 5 to the cavity in the chip body 1 to discharge the liquid, the pressure hammer 201 above the magnetic bead reaction pool 42 is reset, then the pressure hammer 201 is used again to press the solution in the second washing liquid pool 612 through the flow channel 7 to the magnetic bead reaction pool 42, after the second washing, the magnetic beads are adsorbed by the magnet 102, and then the pressure hammer 201 is used again to press the solution in the magnetic bead reaction pool 42 through the liquid discharge groove 5 to the cavity in the chip body 1 to discharge the liquid.

[0070] Then the pressure hammer 201 above the magnetic bead reaction pool 42 is reset, the pressure hammer 201 above the elution liquid pool 62 presses the solution in the elution liquid pool 62 to be discharged into the magnetic bead reaction pool 42, after the nucleic acid is separated from the magnetic beads, the pressure hammer 201 above the magnetic bead reaction pool 42 is pressed to make the solution in the magnetic bead reaction pool 42 pass through the flow channel 7 to be pressed into the quantification pool 43, after the quantification pool 43 is full, the excess solution in the quantification pool 43 flows into the excess liquid pool 63, after the solution in all the magnetic bead reaction pools 42 passes through the flow channel 7 to be pressed into the quantification pool 43, the solution in the quantification pool 43 is still quantified.

[0071] Then the pressure hammer 201 above the quantitative pool 43 extrudes the solution in the quantitative pool 43 into the amplification mixing pool 44, at this time the pressure hammer 201 above the amplification liquid pool 64 and the primer probe pool 65 respectively extrudes the solution in the amplification liquid pool 64 and the primer probe pool 65 into the amplification mixing pool 44 through the flow channel 7, after amplification is completed, the solution in the amplification mixing pool 44 is extruded into the first detection pool 31 through the flow channel 7 by the pressure hammer 201 above the amplification mixing pool 44, the detection solution in the first detection pool 31 can also be extruded into the second detection pool 32 by the pressure hammer 201 above the first detection pool 31, and the detection solution in the second detection pool 32 can also be extruded into the first detection pool 31 by the pressure hammer 201 above the second detection pool 32. Different temperatures in the first detection pool 31 and the second detection pool 32 are used to adapt to different types of nucleic acid for constant temperature amplification for detection.

[0072] The solution is extruded by the pressure hammer 201, so that all the solutions in the reaction pool 4 flow through the flow channel 7, reducing the residue of the solution in the reaction pool 4, and when the pressure hammer 201 extrudes the solution in the reaction pool 4, the solution can flow more quickly. At the same time, there is no need to set up a gas circuit, saving the space for designing a gas circuit, so that the chip body 1 can be set to be lighter and thinner.

[0073] The embodiment also discloses a detection device.

[0074] Referring to Figure 3 and Figure 4 A detection device comprises a lower clamping plate 10 and an upper clamping plate 20, a limiting groove 101 is formed in the lower clamping plate 10, a magnet 102 and an electric heating element 103 are arranged in the limiting groove 101, the magnet 102 is rotationally connected to the lower clamping plate 10, the electric heating element 103 is fixedly connected to the lower clamping plate 10, the upper clamping plate 20 is located on the upper side of the lower clamping plate 10, a plurality of pressure hammers 201 and a plurality of pressure valves 202 are installed on the upper clamping plate 20, the pressure hammers 201 and the pressure valves 202 are provided with driving elements 203, the driving elements 203 are micro air cylinders or linear modules, under the driving of the driving elements 203, the pressure hammers 201 and the pressure valves 202 can move in the vertical direction.

[0075] Referring to Figure 3 and Figure 4 The magnet 102 on the lower clamping plate 10 corresponds to the bottom of the magnetic bead reaction pool 42 and can be close to or away from the bottom of the magnetic bead reaction pool 42 to adsorb or release the magnetic beads in the magnetic bead reaction pool 42. In the embodiment, the magnet 102 is rotationally connected to the lower clamping plate 10, and the rotation of the magnet 102 towards the bottom of the magnetic bead reaction pool 42 or away from the bottom of the magnetic bead reaction pool 42 is controlled by the rotation of the motor.

[0076] Referring toFigure 3 and Figure 4 The electric heating element 103 corresponds to the bottom of the detection cell 3, and the electric heating element 103 is also provided with two, one of which is located below the first detection cell 31, and the other is located below the second detection cell 32, so that the first detection cell 31 and the second detection cell 32 can maintain the required temperature through the two electric heating elements 103.

[0077] Referring to Figure 3 and Figure 4 A plurality of pressure hammers 201 are respectively arranged above the sample lysis cell 41, the magnetic bead reaction cell 42, the quantification cell 43, the residual liquid cell 63, the amplification mixing cell 44, the first detection cell 31, the second detection cell 32, the first washing liquid cell 611, the second washing liquid cell 612, the eluent cell 62, the amplification liquid cell 64 and the primer probe cell 65. Each time the solution is transferred, the pressure hammer 201 can extrude the corresponding solution. The shape of the bottom of the pressure hammer 201 is consistent with the inner wall of the corresponding sample lysis cell 41, magnetic bead reaction cell 42, quantification cell 43, residual liquid cell 63, amplification mixing cell 44, first detection cell 31, second detection cell 32, first washing liquid cell 611, second washing liquid cell 612, eluent cell 62, amplification liquid cell 64 and primer probe cell 65. The surface of the pressure hammer 201 is made of rubber material or high polymer compound and has a certain elasticity, so that the pressure hammer 201 can extrude all the solutions in each cell onto the flow channel 7 when extruding.

[0078] Referring to Figure 3 and Figure 4 A plurality of pressure valves 202 are respectively arranged on the upper side of the flow channels 7 and the liquid discharge groove 5, and the pressure valve 202 is inserted and matched with the corresponding flow channel 7 or liquid discharge groove 5. Under the driving of the corresponding driving member 203, the pressure valve 202 is inserted into the corresponding flow channel 7 or liquid discharge groove 5, at which time the pressure valve 202 closes the corresponding flow channel 7 or the corresponding liquid discharge groove 5. Therefore, the arrangement of the pressure valve 202 can realize the closing or opening of the corresponding flow channel 7 or liquid discharge groove 5.

[0079] Referring to Figure 3 and Figure 4 In an optional embodiment, the lower clamping plate 10 is also provided with an ultrasonic device 104, which is located at the bottom of the sample lysis cell 41. The arrangement of the ultrasonic device 104 facilitates the lysis of nucleic acids in the sample lysis cell 41.

[0080] The implementation principle of the detection equipment in the embodiment is that in the process of detecting nucleic acids, the chip body 1 is placed in the limiting groove 101, and then the sample solution is added into the sample lysis cell 41 through the sample adding hole 2.

[0081] After the sample is lysed, the driving member 203 drives the pressure hammer 201 located on the upper side of the sample lysis pool 41 to extrude the solution in the sample lysis pool 41, and at the same time, the driving member 203 opens the pressure valve 202 between the sample lysis pool 41 and the magnetic bead reaction pool 42, so that the solution in the sample lysis pool 41 is completely transferred to the magnetic bead reaction pool 42, and then the driving member 203 closes the pressure valve 202 between the sample lysis pool 41 and the magnetic bead reaction pool 42.

[0082] After the nucleic acid in the solution in the magnetic bead reaction pool 42 is adsorbed by the magnetic beads, the driving member 203 drives the pressure hammer 201 located on the upper side of the first washing liquid pool 611 to extrude the first washing liquid pool 611, and at the same time, the driving member 203 opens the pressure valve 202 between the first washing liquid pool 611 and the magnetic bead reaction pool 42, so that the solution in the first washing liquid pool 611 is completely transferred to the magnetic bead reaction pool 42, and then the driving member 203 closes the pressure valve 202 between the first washing liquid pool 611 and the magnetic bead reaction pool 42.

[0083] After the first washing is completed, the magnet 102 is close to the bottom of the magnetic bead reaction pool 42, the magnetic beads are adsorbed by the magnet 102, and then the driving member 203 drives the pressure hammer 201 located on the upper side of the magnetic bead reaction pool 42 to extrude the solution in the magnetic bead reaction pool 42, and at the same time, the driving member 203 opens the pressure valve 202 above the liquid discharge groove 5, so that the pressure hammer 201 completely discharges the solution in the magnetic bead reaction pool 42 through the liquid discharge groove 5, and then the driving member 203 closes the pressure valve 202 above the liquid discharge groove 5, and at the same time, the driving member 203 drives the pressure hammer 201 located on the upper side of the magnetic bead reaction pool 42 to reset.

[0084] Then the magnet 102 is away from the bottom of the magnetic bead reaction pool 42, the driving member 203 drives the pressure hammer 201 located on the upper side of the second washing liquid pool 612 to extrude the second washing liquid pool 612, and at the same time, the driving member 203 opens the pressure valve 202 between the second washing liquid pool 612 and the magnetic bead reaction pool 42, so that the solution in the second washing liquid pool 612 is completely transferred to the magnetic bead reaction pool 42, and then the driving member 203 closes the pressure valve 202 between the second washing liquid pool 612 and the magnetic bead reaction pool 42.

[0085] After the second washing is completed, the magnet 102 is close to the bottom of the magnetic bead reaction pool 42, the magnetic beads are adsorbed by the magnet 102, and then the driving member 203 drives the pressure hammer 201 located on the upper side of the magnetic bead reaction pool 42 to extrude the solution in the magnetic bead reaction pool 42, and at the same time, the driving member 203 opens the pressure valve 202 above the liquid discharge groove 5, so that the pressure hammer 201 completely discharges the solution in the magnetic bead reaction pool 42 through the liquid discharge groove 5, and then the driving member 203 closes the pressure valve 202 above the liquid discharge groove 5, and at the same time, the driving member 203 drives the pressure hammer 201 located on the upper side of the magnetic bead reaction pool 42 to reset.

[0086] Then the magnet 102 is moved away from the bottom of the magnetic bead reaction pool 42, the driving member 203 drives the pressure hammer 201 located on the upper side of the eluent pool 62 to extrude the solution in the eluent pool 62, and at the same time the driving member 203 opens the pressure valve 202 between the eluent pool 62 and the magnetic bead reaction pool 42, so that the pressure hammer 201 transfers all the solution in the eluent pool 62 to the magnetic bead reaction pool 42, and then the driving member 203 closes the pressure valve 202 between the eluent pool 62 and the magnetic bead reaction pool 42.

[0087] After the nucleic acid is separated from the magnetic bead, the driving member 203 drives the pressure hammer 201 located on the upper side of the magnetic bead reaction pool 42 to extrude the magnetic bead reaction pool 42, and at the same time the driving member 203 opens the pressure valve 202 between the magnetic bead reaction pool 42 and the quantitative pool 43, and opens the pressure valve 202 between the quantitative pool 43 and the residual liquid pool 63, so that the solution in the magnetic bead reaction pool 42 is all transferred to the quantitative pool 43, and the overflowed solution in the quantitative pool 43 automatically flows into the residual liquid pool 63, and then the driving member 203 closes the pressure valve 202 between the magnetic bead reaction pool 42 and the quantitative pool 43, and closes the pressure valve 202 between the quantitative pool 43 and the residual liquid pool 63.

[0088] The driving member 203 drives the pressure hammer 201 located on the upper side of the quantitative pool 43 to extrude the quantitative pool 43, and at the same time the driving member 203 opens the pressure valve 202 between the quantitative pool 43 and the amplification mixing pool 44, so that the solution in the quantitative pool 43 is all transferred to the amplification mixing pool 44, and then the driving member 203 closes the pressure valve 202 between the magnetic bead reaction pool 42 and the amplification mixing pool 44.

[0089] Then the driving member 203 drives the pressure hammer 201 located on the upper side of the amplification liquid pool 64 to extrude the amplification liquid pool 64, and at the same time the driving member 203 opens the pressure valve 202 between the amplification liquid pool 64 and the amplification mixing pool 44, so that the solution in the amplification liquid pool 64 is all transferred to the amplification mixing pool 44, and then the driving member 203 closes the pressure valve 202 between the amplification liquid pool 64 and the amplification mixing pool 44.

[0090] Then the driving member 203 drives the pressure hammer 201 located on the upper side of the primer probe pool 65 to extrude the primer probe pool 65, and at the same time the driving member 203 opens the pressure valve 202 between the primer probe pool 65 and the amplification mixing pool 44, so that the solution in the primer probe pool 65 is all transferred to the amplification mixing pool 44, and then the driving member 203 closes the pressure valve 202 between the primer probe pool 65 and the amplification mixing pool 44.

[0091] After the nucleic acid is amplified, the driving member 203 drives the pressure hammer 201 located on the upper side of the amplification liquid pool 64 to extrude the amplification mixing pool 44, and at the same time, the driving member 203 opens the pressure valve 202 between the amplification mixing pool 44 and the first detection pool 31, so that all the solution in the amplification mixing pool 44 is transferred into the first detection pool 31, and then the driving member 203 closes the pressure valve 202 between the amplification mixing pool 44 and the first detection pool 31.

[0092] When it is needed to be transferred into the first detection pool 31, the driving member 203 drives the pressure hammer 201 located on the upper side of the first detection pool 31 to extrude the first detection pool 31, and at the same time, the driving member 203 opens the pressure valve 202 between the first detection pool 31 and the second detection pool 32, so that all the solution in the first detection pool 31 is transferred into the second detection pool 32, and then the driving member 203 closes the pressure valve 202 between the first detection pool 31 and the second detection pool 32.

[0093] And when it is needed to transfer the solution in the second detection pool 32 into the first detection pool 31, the driving member 203 drives the pressure hammer 201 located on the upper side of the second detection pool 32 to extrude the second detection pool 32, and at the same time, the driving member 203 opens the pressure valve 202 between the first detection pool 31 and the second detection pool 32, so that all the solution in the second detection pool 32 is transferred into the first detection pool 31, and then the driving member 203 closes the pressure valve 202 between the first detection pool 31 and the second detection pool 32.

[0094] The solution is extruded by the pressure hammer 201, so that all the solution in the reaction pool 4 flows through the flow channel 7, which accelerates the flow speed of the solution and makes the experimental process more rapid.

[0095] Embodiment 2: The embodiment discloses a detection device, referring to Figure 5 The difference between the embodiment and the embodiment 1 is that the mounting seat 30 is arranged on the upper side of the lower clamping plate 10, and the mounting seat 30 is detachably connected with the lower clamping plate 10, the limiting groove 101 is arranged on the upper side of the mounting seat 30 and penetrates through the mounting seat 30 along the length direction of the limiting groove 101, the chip body 1 is inserted and matched with the limiting groove 101, the length direction of the chip body 1 is arranged along the length direction of the limiting groove 101, and the chip body 1 is slidably connected with the inner side wall of the limiting groove 101 along the length direction of the chip body 1.

[0096] Referring to Figure 5 and Figure 6The limiting groove 101 is provided with a limiting assembly 40 on opposite sides, the limiting assembly 40 comprises a mounting body 401, mounting holes 402 are provided on opposite inner side walls of the limiting groove 101, the mounting body 401 is inserted and matched with the mounting holes 402, and the mounting body 401 is connected with the mounting seat 30 through bolts, and a side of the mounting body 401 facing the chip body 1 is flush with the corresponding inner side wall of the limiting groove 101. The mounting body 401 and the mounting holes 402 are matched and arranged, so that the limiting assembly 40 is convenient to mount and dismount.

[0097] With reference to Figure 5 and Figure 6 The limiting assembly 40 further comprises an abutting piece 403, the abutting piece 403 comprises a limiting block 4031, a sliding hole 4032 is provided on a side of the mounting body 401 facing the chip body 1, and the limiting block 4031 is located in the sliding hole 4032, the limiting block 4031 is inserted and matched with the sliding hole 4032, and the limiting block 4031 is slidingly connected with the inner side wall of the sliding hole 4032 along the axial direction of the sliding hole 4032.

[0098] With reference to Figure 5 and Figure 6 A side of the chip body 1 along the length direction thereof abuts against the limiting block 4031, and the other side abuts against the corresponding inner side wall of the limiting groove 101. When the limiting block 4031 extends out of the sliding hole 4032, the limiting block 4031 can limit the chip body 1.

[0099] With reference to Figure 5 and Figure 6 A side of the limiting block 4031 away from the chip body 1 is provided with a guide surface 4033, the guide surface 4033 is obliquely arranged, and the guide surface 4033 is obliquely arranged towards the chip body 1 along a side of the mounting body 401 away from the axial direction of the sliding hole 4032. When the chip body 1 is inserted, the chip body 1 abuts against the guide surface 4033, at this time, the limiting block 4031 is retracted into the sliding hole 4032 under the abutment of the chip body 1, which can prevent the limiting block 4031 from interfering with the installation of the chip body 1.

[0100] With reference to Figure 6 and Figure 7 The abutting piece 403 further comprises a first spring 4034, the first spring 4034 is arranged along the axial direction of the sliding hole 4032 in the length direction, one end of the first spring 4034 is connected with the limiting block 4031, and the other end is connected with the inner side wall of the sliding hole 4032. The first spring 4034 is arranged, when the chip body 1 abuts against the limiting block 4031 and is retracted into the sliding hole 4032, the first spring 4034 is compressed, and then when the chip body 1 is separated from the limiting block 4031, the first spring 4034 resets the limiting block 4031, at this time, the chip body 1 is located between the limiting block 4031 and the corresponding inner side wall of the limiting groove 101 along the length direction thereof, which can realize the automatic fixing of the chip body 1.

[0101] With reference to Figure 5 and Figure 7 , the limiting assembly 40 further comprises a manual driving member 404, the manual driving member 404 comprises a driving column 4041, the installation main body 401 is provided with a driving hole 4042 on the side away from the limiting slot 101, the driving hole 4042 and the sliding hole 4032 are respectively located on the opposite sides of the installation main body 401, and the axes of the driving hole 4042 and the sliding hole 4032 are arranged in parallel with each other. One end of the driving column 4041 is inserted into the driving hole 4042 in a fit manner, the other end of the driving column 4041 extends to the outside of the installation main body 401, and the driving column 4041 is slidably connected with the inner side wall of the driving hole 4042 along the length direction of the driving column 4041. The manual driving member 404 further comprises a second spring 4043, the second spring 4043 is located in the driving hole 4042, the length direction of the second spring 4043 is arranged along the axial direction of the driving hole 4042, one end of the second spring 4043 is connected with the driving column 4041, and the other end of the second spring 4043 is connected with the inner side wall of the driving hole 4042.

[0102] By pressing the driving column 4041 along the axial direction of the driving column 4041, the driving column 4041 can be pushed into the driving hole 4042, and after the driving column 4041 is released, the second spring 4043 can drive the driving column 4041 to reset.

[0103] With reference to Figure 5 and Figure 7 , the limiting assembly 40 further comprises a conversion member 405, the conversion member 405 comprises an intermediate rod 4051, the installation main body 401 is provided with an installation cavity 4011, the intermediate rod 4051 is located in the installation cavity 4011, the intermediate rod 4051 is rotatably connected with the installation main body 401 at the middle part, one end of the intermediate rod 4051 is rotatably connected with the driving column 4041, the other end of the intermediate rod 4051 is rotatably connected with the limiting block 4031, and the limiting block 4031 and the driving column 4041 are slidably connected with the intermediate rod 4051 along the length direction of the intermediate rod 4051.

[0104] When the driving column 4041 is pressed and shrunk into the driving hole 4042, under the action of the intermediate rod 4051, the limiting block 4031 is also shrunk into the sliding hole 4032, at this time, the limiting block 4031 releases the locking of the chip body 1, which facilitates the chip body 1 to be taken off from the mounting seat 30.

[0105] With reference to Figure 7The inner side wall of the sliding hole 4032 is provided with a first long hole 4052, the length direction of the first long hole 4052 is arranged along the axial direction of the sliding hole 4032, and the first long hole 4052 is in communication with the mounting cavity 4011. The conversion piece 405 includes a first column 4053, the first column 4053 is in plug-in cooperation with the first long hole 4052, one end of the first column 4053 is connected with the limiting block 4031, the other end of the first column 4053 is rotationally connected with the intermediate rod 4051, the first column 4053 is slidingly connected with the intermediate rod 4051 along the length direction of the intermediate rod 4051, and the first column 4053 is slidingly connected with the inner side wall of the first long hole 4052 along the length direction of the first long hole 4052, so that the rotation connection between the limiting block 4031 and the intermediate rod 4051 is realized.

[0106] With reference to Figure 7 The inner side wall of the driving hole 4042 is provided with a second long hole 4054, the length direction of the second long hole 4054 is arranged along the axial direction of the driving hole 4042, and the second long hole 4054 is in communication with the mounting cavity 4011. The rotating piece further includes a second column 4055, the second column 4055 is in plug-in cooperation with the second long hole 4054, one end of the second column 4055 is connected with the driving column 4041, the other end of the second column 4055 is rotationally connected with the intermediate rod 4051, and the second column 4055 is slidingly connected with the intermediate rod 4051 along the length direction of the intermediate rod 4051. This realizes the rotation connection between the driving column 4041 and the intermediate rod 4051.

[0107] With reference to Figure 7 The conversion piece 405 further includes a mounting branch rod 4012, the length direction of the mounting branch rod 4012 is arranged along the axial direction of the sliding hole 4032, one end of the mounting branch rod 4012 is connected with the inner wall of the mounting cavity 4011, and the other end of the mounting branch rod 4012 is rotationally connected with the middle part of the intermediate rod 4051. Through the arrangement of the mounting branch rod 4012, the installation of the intermediate rod 4051 can be realized.

[0108] With reference to Figure 7 And Figure 8 The inner side wall of the limiting groove 101 is further provided with a pop-up assembly 50, the pop-up assembly 50 is located on the side of the chip body 1 away from the limiting block 4031 along the length direction of the chip body 1. The pop-up assembly 50 includes a pop-up plate 501, the inner side wall of the limiting groove 101 is provided with a pop-up groove 502, the pop-up plate 501 is in plug-in cooperation with the pop-up groove 502, the pop-up plate 501 is slidingly connected with the inner side wall of the pop-up groove 502 along the depth direction of the pop-up groove 502, a plurality of pop-up springs 503 are arranged between the pop-up plate 501 and the groove bottom of the pop-up groove 502, the axial direction of the pop-up spring 503 is arranged along the depth direction of the limiting groove 101, one end of the pop-up spring 503 is connected with the groove bottom of the limiting groove 101, and the other end of the pop-up spring 503 is connected with the pop-up plate 501.

[0109] When the chip body 1 is installed into the limiting groove 101, the chip body 1 is in contact with the ejection plate 501 and is retracted into the ejection groove 502, at this time, the ejection spring 503 is compressed, and the chip body 1 is located between the ejection plate 501 and the limiting block 4031 along the length direction of the chip body 1. When the chip body 1 needs to be removed, the driving column 4041 is pressed, the limiting block 4031 is retracted into the sliding hole 4032 under the action of the middle rod 4051, at this time, the limiting block 4031 contacts the limiting of the chip body 1, the ejection spring 503 is in contact with the ejection plate 501, and the ejection plate 501 ejects the chip body 1, which facilitates the removal of the chip body 1.

[0110] Referring to Figure 5 The mounting seat 30 is in sliding connection with the lower clamping plate 10, and a mounting assembly 60 is arranged between the mounting seat 30 and the lower clamping plate 10. The mounting assembly 60 comprises a plurality of mounting bolts 601. Long mounting holes 602 are formed on both sides of the mounting seat 30 in the width direction. The length direction of the long mounting holes 602 is arranged along the length direction of the mounting seat 30. A plurality of threaded holes are formed on the lower clamping plate 10. The plurality of threaded holes are in communication with the corresponding long mounting holes 602. The mounting bolts 601 are arranged in one-to-one correspondence with the threaded holes. The mounting bolts 601 are in plug-in fit and threaded connection with the corresponding threaded holes. The nuts of the mounting bolts 601 are in contact with the upper side of the mounting seat 30. When the mounting bolts 601 are tightened, the mounting seat 30 is fixed. When the mounting bolts 601 are loosened, the position of the mounting seat 30 is adjusted.

[0111] The implementation principle of the detection equipment according to the embodiment of the application is as follows: in the process of checking the nucleic acid, one end of the chip body 1 in the length direction is inserted into the limiting groove 101, and then the chip body 1 is pushed along the length direction of the chip body 1 until the chip body 1 is completely inserted into the limiting groove 101. At this time, the limiting block 4031 fixes the position of the chip body 1, and the ejection plate 501 can abut against the chip body 1, so that the chip body 1 cannot shake or move in the limiting groove 101, which can improve the stability and position accuracy of the installation of the chip body 1. After that, the driving columns 4041 protruding on both sides of the mounting seat 30 are pressed, the limiting block 4031 releases the limiting of the chip body 1, and the ejection plate 501 ejects the chip body 1 from the limiting groove 101, which realizes the disassembly of the chip body 1.

[0112] The above are preferred embodiments of the application, which do not limit the protection scope of the application. Therefore, equivalent changes made on the basis of the structure, shape and principle of the application should be covered by the protection scope of the application.

Claims

1. A pressure-driven microfluidic chip, characterized in that, Include: Chip body (1), the inside of the chip body (1) has a cavity, the chip body (1) is provided with sample addition hole (2), detection pool (3), multiple reaction pool (4), multiple reagent pool (6) and multiple flow channel (7); Multiple reaction pool (4) includes sample lysing pool (41), magnetic bead reaction pool (42) and amplification mixing pool (44) communicated in sequence, and the sample addition hole (2) is communicated with the sample lysing pool (41), the detection pool (3) is communicated with the amplification mixing pool (44), the bottom of the magnetic bead reaction pool (42) is provided with magnetic bead, the side of the opening of the magnetic bead reaction pool (42) is also provided with drain groove (5), and the drain groove (5) is communicated with the cavity inside the chip body (1); The reaction pool (4) further includes a quantitative pool (43), the quantitative pool (43) is located between the magnetic bead reaction pool (42) and the amplification mixing pool (44), one side of the quantitative pool (43) is communicated with the magnetic bead reaction pool (42), and the other side is communicated with the amplification mixing pool (44); Multiple reagent pool (6) includes washing liquid pool (61), eluent pool (62), amplification liquid pool (64) and primer probe pool (65), the washing liquid pool (61) and the eluent pool (62) are communicated with the magnetic bead reaction pool (42) respectively, the amplification liquid pool (64) and the primer probe pool (65) are communicated with the amplification mixing pool (44) respectively; The reagent pool (6) further includes a residual liquid pool (63), and the residual liquid pool (63) is communicated with the quantitative pool (43); Multiple flow channels (7) are used to communicate the openings between adjacent reaction pools (4), and also used to communicate the openings of the reagent pool (6) with the openings of the corresponding reaction pool (4); When the solution in the corresponding reaction pool (4) or the reagent pool (6) is extruded by the pressure hammer (201), the solution in the reaction pool (4) or the reagent pool (6) flows into the corresponding adjacent reaction pool (4) through the corresponding flow channel (7).

2. The pressure-driven microfluidic chip according to claim 1, wherein: The bottom of the reaction pool (4) and the reagent pool (6) is spherical, and the inner concave side faces the opening direction of the reaction pool (4).

3. The pressure-driven microfluidic chip according to claim 2, wherein: The inner wall of the reaction pool (4) and the reagent pool (6) is provided with drainage groove (8), and one side of the drainage groove (8) is communicated with the flow channel (7) on one side of the flow direction.

4. The pressure-driven microfluidic chip according to claim 3, wherein: Multiple drainage grooves (8) are provided, and multiple drainage grooves (8) are communicated with the flow channel (7) on one side of the flow direction.

5. The pressure-driven microfluidic chip of claim 4, wherein: The side of the same drainage groove (8) away from the flow channel (7) is located on the same contour surface.

6. The pressure-driven microfluidic chip of claim 1, wherein: The detection pool (3) includes first detection pool (31) and second detection pool (32), and the amplification mixing pool (44), the first detection pool (31) and the second detection pool (32) are communicated in sequence.

7. The pressure-driven microfluidic chip of claim 1, wherein: The washing liquid pool (61) comprises a first washing liquid pool (611) and a second washing liquid pool (612), and the first washing liquid pool (611) and the second washing liquid pool (612) are communicated with the magnetic bead reaction pool (42) respectively.

8. A detection device, characterized in that Comprise: The pressure type microfluidic chip according to any one of claims 1-7; A lower clamping plate (10) is provided with a limiting groove (101) for accommodating the chip body (1); A magnet (102) is rotatably connected to the lower clamping plate (10) to move away from or close to the bottom of the magnetic bead reaction pool (42); An electric heating element (103) is fixedly connected to the lower clamping plate (10) and located at the bottom of the detection pool (3); An upper clamping plate (20) is located on the upper side of the lower clamping plate (10), and the upper clamping plate (20) is provided with a plurality of pressure hammers (201), a plurality of pressure valves (202), and a driving element (203) for driving the pressure hammers (201) and the pressure valves (202) to move; The pressure hammers (201) are respectively arranged one-to-one corresponding to the reaction pools (4), the reagent pools (6), and the detection pools (3), and the shape of the pressure hammers (201) is consistent with the inner wall of the corresponding reaction pools (4), reagent pools (6), and detection pools (3); The pressure valves (202) are respectively arranged one-to-one corresponding to the flow-through grooves (7) and the liquid discharge grooves (5), and the pressure valves (202) are used for closing or opening the corresponding flow-through grooves (7) or liquid discharge grooves (5).

9. The detection device of claim 8, wherein, The lower clamping plate (10) is further provided with an ultrasonic device (104) located at the bottom of the sample lysis pool (41).

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