Intelligent microfluidic chemical reaction platform based on FPGA

Abstract

The invention discloses an intelligent microfluidic chemical reaction platform based on an FPGA (Field Programmable Gate Array), which comprises an FPGA core control board, a user service module, a video coding module, an industrial control board and a microfluidic reaction equipment unit, and is characterized in that the user service module, the video coding module and the industrial control board are controlled by the FPGA core control board; the chemical reaction equipment unit is accurately, efficiently and intelligently controlled, micro-fluidic chemical reaction is performed according to user requirements, and a teaching demonstration function is also considered. According to the invention, control and monitoring of various reaction parameters such as temperature, pressure, quality, pH and the like are integrated, intelligent microfluidic reaction integrated control is realized, two image sensors are used for real-time monitoring of an experimental process and video storage of a reaction process in a reaction chip, and complete storage of reaction data is ensured. The system has the advantages of being reasonable in structure, high in working freedom degree, convenient to control, good in compatibility and high in interaction capacity, and is suitable for various scenes such as classroom experiment teaching, student exploration experiments and college laboratory scientific research.

Description

Technical Field

[0001] The present invention relates to the technical fields of continuous flow chemistry, network communication technology, and high-speed electronic circuit technology, and particularly relates to an intelligent microfluidic chemical reaction platform based on FPGA, which is applicable to the automatic control of reaction conditions in flow chemistry experiments, and the acquisition, transmission, and analysis of experimental data and video data. Background Art

[0002] Continuous flow chemistry refers to chemical reaction equipment and methods in which two or more different reactants are pumped into a single chamber, tube, or microreactor at a specific flow rate. After the reaction is completed, the stream containing the resulting compound is collected at the outlet, or it is directed to a subsequent flow reactor loop for further reaction to obtain the final product. Network communication technology refers to the technology of collecting, storing, processing, and transmitting data through a computer network system to achieve the full sharing of information resources. Flow chemistry has been widely applied in many fields such as pharmaceuticals and fine chemicals, but the popularity of the concept of flow chemistry in the field of chemical education has not kept up, and traditional batch reaction methods are still mostly used for experiments, with low system integration. Considering the growing demand for efficient, safe, and green chemistry in chemical research and industrial production, it is particularly important to introduce flow chemistry knowledge in experimental teaching, combine continuous flow chemistry technology with network communication technology and high-speed electronic circuit technology, and design an intelligent microfluidic chemical reaction platform with rich functions, convenient operation, and excellent performance.

[0003] The key points that a flow chemistry experimental platform needs to solve are as follows: First, to construct a system with high universality and simple operation. Through replaceable module design, it can be compatible with a variety of reactants, reaction types, and reaction conditions, ensuring simple and efficient switching between different experiments and meeting the needs of demonstrating and exploring various reaction types in teaching. Second, to ensure the coordinated improvement of automation and safety, the reaction parameters can be accurately set and adjusted, and the reaction process and result data can be fed back in real time, monitoring the real-time dynamics during the experimental process of the reaction platform, and archiving and storing the video of the reaction phenomenon for students to deeply understand the reaction principle and influencing factors and to review past experiments in a timely manner. Moreover, multiple safety monitoring and protection mechanisms are embedded to ensure that the experimental process can operate safely and reliably even without direct human monitoring. Third, to design a controller with highly expandable functions. Construct an open programming interface and a modular functional architecture to facilitate the subsequent addition of new control algorithms, data processing functions, and connection to other devices for post-processing, laying a foundation for cultivating talents who meet the needs of modern chemical development. Summary of the Invention

[0004] The object of the present invention is to provide an FPGA-based intelligent microfluidic chemical reaction platform in view of the deficiencies of the prior art. The present invention integrates an FPGA core control board, a user service module, a video encoding module, an industrial control board, and a flow chemistry equipment module. The fiber optic Ethernet communication module of the FPGA core control board receives the control instructions sent by the user service module and accurately transmits the instructions to the industrial control board and the video encoding module, realizing precise control of the flow chemistry equipment module and real-time acquisition and storage of experimental video data. Experimental personnel can precisely control each component of the entire reaction platform through the user service module, quantitatively adjust each experimental parameter of the flow chemical reaction system. At the same time, they can also monitor the real-time dynamics of the reaction platform and retrieve past experimental videos for comparative research. The present invention has the advantages of reasonable structure, high working freedom, portable control, good compatibility, and strong interaction ability.

[0005] The specific technical solution for achieving the object of the present invention is as follows: An FPGA-based intelligent microfluidic chemical reaction platform, which includes an FPGA core control board, a user service module, a video encoding module, an industrial control board, and a flow chemistry equipment module; The FPGA core control board is connected to the user service module, the video encoding module, the industrial control board, and the flow chemistry equipment module, and is used to receive and parse the instruction data sent by the user service module and upload the experimental data collected in real time during the reaction process of the flow chemistry equipment module to the industrial control board, and receive the encoded experimental monitoring video of the flow chemistry equipment module output by the video encoding module; The user service module is connected to the FPGA core control board, and is used to receive the experimental data and the monitoring video sent by the FPGA core control board, and send the user operation instructions to the FPGA core control board; The video encoding module is connected to the FPGA core control board and the flow chemistry equipment module, and is used to obtain the monitoring video during the experiment of the flow chemistry equipment module and encode the video with H.265 and then transmit it to the FPGA core control board for storage; The industrial control board is connected to the FPGA core control board and the flow chemistry equipment module, and is used to receive the control instructions sent by the FPGA core control board, collect the values of each experimental parameter in real time during the reaction process of the flow chemistry equipment module and transmit them to the FPGA core control board for processing; The flow chemistry equipment module is connected to the FPGA core control board and the industrial control board, and is used to receive the control signal sent by the industrial control board and execute it accurately, and the reaction process image is collected in real time by the FPGA core control board.

[0006] The FPGA core control board includes a clock module, a power supply module, a fiber optic Ethernet communication module, a MIPI image sensor module, a microfluidic chemical reaction control module, a video storage module, a video output module, a data cache module, and a gigabit Ethernet communication module; The clock module is connected to the power supply module, the fiber optic Ethernet communication module, the video monitoring module, the microfluidic chemical reaction control module, the video storage module, the video output module, the data cache module, and the gigabit Ethernet communication module, providing a reference clock for each module; The power supply module is connected to the clock module, the fiber optic Ethernet communication module, the MIPI image sensor module, the microfluidic chemical reaction control module, the video storage module, the video output module, the data cache module, and the gigabit Ethernet communication module, supplying power to each module; The fiber optic Ethernet communication module includes a fiber optic Ethernet data receiving module and a fiber optic Ethernet data sending module, and is connected to the clock module, the power supply module, the microfluidic chemical reaction control module, the video output module, the gigabit Ethernet communication module, and the user service module. It receives the operation instructions sent by the user service module through the fiber optic Ethernet data receiving module and transmits the operation instructions to the gigabit Ethernet communication module and the microfluidic chemical reaction control module; the fiber optic Ethernet data sending module receives the video signals output by the video output module and the judgment results of various experimental data collected by the microfluidic chemical reaction control module on the experimental progress, and forms the above data into a TCP data packet format and sends it to the user service module; The video monitoring module includes a MIPI interface module and an image sensor module, and is connected to the clock module, the power supply module, the flow chemistry device module, and the video output module. The image sensor module collects the images during the experiment of the flow chemistry device module in real time and transmits them to the video output module through the MIPI interface module for output display, which is used for observing and demonstrating the experimental process; The microfluidic chemical reaction control module includes a reaction progress judgment module, a real-time parameter acquisition module, a power-on system status detection module, and a control signal generation module, and is connected to the clock module, the power supply module, the fiber optic Ethernet data receiving module, the fiber optic Ethernet data sending module, and the industrial control board. The reaction progress judgment module judges the progress of the reaction according to the experimental process parameters collected by the real-time parameter acquisition module and transmits the result to the fiber optic Ethernet data sending module. The real-time parameter acquisition module directly transmits the collected experimental process parameters to the fiber optic Ethernet data sending module; the power-on system status detection module generates initialization check instructions for each component of the flow chemistry device module when the system is powered on, and generates corresponding control signals through the control signal generation module to check the status of the initially powered-on system to ensure that the functions of each component are normal; The video storage module includes a SATA hard disk module and a SATA interface module, which are connected to a clock module, a power module, a video output module, and a data cache module. The SATA interface module receives the encoded reaction video data transmitted from the data cache module and stores it in the SATA hard disk module. Meanwhile, the stored reaction video data is sent to the video output module for decoding and output. The video output module includes an H.265 video decoding module and an HDMI interface module, which are connected to a clock module, a power module, a fiber optic Ethernet sending module, an MIPI interface module, and a SATA interface module. It allows receiving the unencoded real-time monitoring video data transmitted by the MIPI interface module and outputting it through the HDMI interface module. It can also receive the reaction video data stored in the SATA hard disk module transmitted by the SATA interface module, decode it through the H.265 video decoding module, and then transmit it to the HDMI interface module or the fiber optic Ethernet data sending module. The data cache module includes a DDR4 data cache module and a DDR4 data control module, which are connected to a clock module, a power module, a SATA interface module, and a gigabit Ethernet communication module. The DDR4 data cache module caches the encoded reaction video data transmitted by the gigabit Ethernet communication module in real time at high speed. The DDR4 data control module transmits the real-time cached data to the SATA interface module according to the timing requirements of the SATA interface. The gigabit Ethernet communication module includes a gigabit Ethernet sending module and a gigabit Ethernet receiving module, which are connected to a clock module, a power module, a fiber optic Ethernet data receiving module, an H.265 video encoding module, and a DDR4 data cache module. The gigabit Ethernet sending module receives the user instructions forwarded by the fiber optic Ethernet data receiving module and sends them to the H.265 video encoding module. The gigabit Ethernet receiving module receives the encoded reaction video data transmitted by the H.265 video encoding module and sends it to the DDR4 data cache module for real-time high-speed caching.

[0007] The user service module includes a user operation interface and a user data storage module.

[0008] The user operation interface is connected to the fiber optic Ethernet data receiving module and the user data storage module, used to generate user operation instructions and send them to the fiber optic Ethernet data receiving module, read the reaction parameters and video data from the user data storage module and display them to enhance user interaction. The data storage module is connected to the fiber optic Ethernet data sending module, used to receive and store reaction parameters and video data.

[0009] The video encoding module includes an image sensor module, an MIPI interface module, and an H.265 video encoding module. The image sensor module is connected to the MIPI interface module and the flow chemistry device module, and collects reaction video data in real time and transmits it to the MIPI interface module; The MIPI interface module is connected to the image sensor module and the H.265 video encoding module, and transmits the reaction video data collected by the image sensor module to the H.265 video encoding module; The H.265 video encoding module is connected to the gigabit Ethernet receiving module and the MIPI interface module, receives the unencoded reaction video data transmitted by the MIPI interface module, performs H.265 encoding, and then outputs it to the gigabit Ethernet receiving module.

[0010] The industrial control board includes an RS module, a PWM output module, a relay matrix module, an ADC sampling module, and a motor drive module; The RS module is connected to the real-time parameter acquisition module, the control signal generation module, and the flow chemistry device module, receives the control signal sent by the control signal generation module, precisely controls the relevant flow chemistry devices, and at the same time transmits the feedback parameters of the relevant devices to the real-time parameter acquisition module; The PWM output module is connected to the control signal generation module and the flow chemistry device module, receives the control signal, and generates corresponding PWM waves to control the relevant devices in the flow chemistry device module; The relay matrix module is connected to the control signal generation module and the flow chemistry device module, receives the control signal, and performs switching operations on the relay matrix to control the relevant devices in the flow chemistry device module; The ADC sampling module is connected to the real-time parameter acquisition module, the control signal generation module, and the flow chemistry device module, receives the control signal, and performs real-time sampling on each experimental parameter in the flow chemistry device module, and transmits the sampling data to the real-time parameter acquisition module; The motor drive module is connected to the control signal generation module and the flow chemistry device module, receives the control signal, generates a motor control signal, and drives the motor in the flow chemistry device module to work precisely.

[0011] The beneficial effects of the present invention are as follows: (1) The present invention uses an FPGA hardware architecture to process high-bandwidth network data and complex control instructions. Compared with the traditional ARM chip architecture, it has a higher processing speed and stronger parallel processing ability.

[0012] (2) The present invention takes into account each reaction parameter in the flow chemical reaction and the control of experimental equipment, and has a complete system structure and high integration.

[0013] (3) The present invention integrates a system power-on automatic detection module, and performs self-checks on all modules of the entire system during initial power-on to ensure the safety and efficiency of the experimental process.

[0014] (4) The video of the flow chemical reaction of the present invention is encoded and stored, which is convenient for subsequent comparative experiment learning, and is of great significance for teaching and research.

[0015] (5) The present invention establishes a complete database system to record the experimental data in detail, with strong data reliability and high integrity.

[0016] (6) The present invention realizes the integrated control under different demand scenarios of flow chemical reactions, with strong versatility and strong compatibility. Description of the Drawings

[0017] Figure 1 is the architecture diagram of the present invention; Figure 2 is the working flow diagram of the present invention; Figure 3 is the working flow diagram of the flow chemical equipment module when the present invention is conducting experiments.

[0018] In the figure: 1 - FPGA core control board; 2 - user service module; 3 - video encoding module; 4 - industrial control board; 5 - flow chemical equipment module; 11 - clock module; 12 - power supply module; 13 - fiber optic Ethernet communication module; 14 - MIPI image sensor module; 15 - microfluidic chemical reaction control module; 16 - video storage module; 17 - video output module; 18 - data cache module; 19 - gigabit Ethernet communication module; 21 - user operation interface; 22 - user data storage module; 31 - image sensor module; 32 - MIPI interface module; 33 - H.265 video encoding module; 41 - RS485 module; 42 - PWM output module; 43 - relay matrix module; 44 - ADC sampling module; 45 - motor drive module; 51 - optoelectronic bubble sensor; 52 - liquid storage bottle array; 53 - vertical injection pump; 54 - electromagnetic switching valve; 55 - pressure sensor; 56 - temperature control module; 57 - flow chemical reaction chip; 58 - temperature sensor; 59 - back pressure valve; 510 - pH sensor; 511 - product liquid storage bottle; 512 - mass sensor; 513 - waste liquid storage bottle; 131 - fiber optic Ethernet data receiving module; 132 - fiber optic Ethernet data sending module 132; 141 - MIPI interface module; 142 - image sensor module; 151 - reaction progress judgment module; 152 - real-time parameter acquisition module; 153 - power-on system status detection module; 154 - control signal generation module; 161 - SATA hard disk module; 162 - SATA interface module; 171 - H.265 video decoding module; 172 - HDMI interface module; 181 - DDR4 data cache module; 182 - DDR4 data control module; 191 - gigabit Ethernet sending module; 192 - gigabit Ethernet receiving module. Detailed implementation mode

[0019] The present invention will be described in detail below with reference to the accompanying drawings and embodiments.

[0020] Refer to Figure 1 , this embodiment includes an FPGA core control board 1, a user service module 2, a video encoding module 3, an industrial control board 4, and a flow chemistry equipment module 5; The FPGA core control board 1 is connected to the user service module 2, the video encoding module 3, the industrial control board 4, and the flow chemistry equipment module 5; The user service module 2 is connected to the FPGA core control board 1; The video encoding module 3 is connected to the FPGA core control board 1 and the flow chemistry equipment module 5; The industrial control board 4 is connected to the FPGA core control board 1 and the flow chemistry equipment module 5; The flow chemistry equipment module 5 is connected to the FPGA core control board 1 and the industrial control board 4.

[0021] Refer to Figure 1, the FPGA core control board 1 includes a clock module 11, a power supply module 12, a fiber optic Ethernet communication module 13, a MIPI image sensor module 14, a microfluidic chemical reaction control module 15, a video storage module 16, a video output module 17, a data cache module 18, and a gigabit Ethernet communication module 19; the clock module 11 is connected to the power supply module 12, the fiber optic Ethernet communication module 13, the video monitoring module 14, the microfluidic chemical reaction control module 15, the video storage module 16, the video output module 17, the data cache module 18, and the gigabit Ethernet communication module 19 to provide a reference clock for each module; the power supply module 12 is connected to the clock module 11, the fiber optic Ethernet communication module 13, the MIPI image sensor module 14, the microfluidic chemical reaction control module 15, the video storage module 16, the video output module 17, the data cache module 18, and the gigabit Ethernet communication module 19 to supply power to each module; the fiber optic Ethernet communication module 13 includes a fiber optic Ethernet data receiving module 131 and a fiber optic Ethernet data sending module 132, and is connected to the clock module 11, the power supply module 12, the microfluidic chemical reaction control module 15, the video output module 17, the gigabit Ethernet communication module 19, and the user service module 2. The fiber optic Ethernet data receiving module 131 receives the operation instructions sent by the user service module 2 and transmits the operation instructions to the gigabit Ethernet communication module 19 and the microfluidic chemical reaction control module 15; the fiber optic Ethernet data sending module 132 receives the video signal output by the video output module 17 and the judgment results of various experimental data collected by the microfluidic chemical reaction control module 15 on the experimental progress, and forms the above data into a TCP data packet format and sends it to the user service module 2; the video monitoring module 14 includes a MIPI interface module 141 and an image sensor module 142, and is connected to the clock module 11, the power supply module 12, the flow chemistry device module 5, and the video output module 17. The image sensor module 142 collects the images during the experiment of the flow chemistry device module 5 in real time and transmits them to the video output module 17 through the MIPI interface module 141 for output display, which is used for observing and demonstrating the experimental process; the microfluidic chemical reaction control module 15 includes a reaction progress judgment module 151, a real-time parameter collection module 152, a power-on system status detection module 153, and a control signal generation module 154, and is connected to the clock module 11, the power supply module 12, the fiber optic Ethernet data receiving module 131, the fiber optic Ethernet data sending module 132, and the industrial control board 4. The reaction progress judgment module 151 judges the progress of the reaction according to the experimental process parameters collected by the real-time parameter collection module 152 and transmits the result to the fiber optic Ethernet data sending module 132. The real-time parameter collection module 152 directly transmits the collected experimental process parameters to the fiber optic Ethernet data sending module 132;The power-on system status detection module 153 generates initialization check instructions for each component of the flow chemistry equipment module 5 when the system is powered on, and generates corresponding control signals through the control signal generation module 154 to check the status of the initially powered-on system to ensure that the functions of each component are normal; the video storage module 16 includes a SATA hard disk module 161 and a SATA interface module 162, and is connected to the clock module 11, the power supply module 12, the video output module 17, and the data cache module 18. The SATA interface module 162 receives the encoded reaction video data transmitted from the data cache module 18 and stores it in the SATA hard disk module 161, and at the same time sends the stored reaction video data to the video output module 17 for decoding and output; the video output module 17 includes an H.265 video decoding module 171 and an HDMI interface module 172, and is connected to the clock module 11, the power supply module 12, the fiber optic Ethernet transmission module 132, the MIPI interface module 141, and the SATA interface module 162. It allows receiving the unencoded real-time monitoring video data transmitted by the MIPI interface module 141 and outputting it through the HDMI interface module 172, and can also receive the reaction video data stored in the SATA hard disk module 161 transmitted by the SATA interface module 162. After being decoded by the H.265 video decoding module 171, it is transmitted to the HDMI interface module 172 or the fiber optic Ethernet data transmission module 132; the data cache module 18 includes a DDR4 data cache module 181 and a DDR4 data control module 182, and is connected to the clock module 11, the power supply module 12, the SATA interface module 162, and the gigabit Ethernet communication module 19. The DDR4 data cache module 181 caches the encoded reaction video data transmitted from the gigabit Ethernet communication module 19 in real time at high speed, and the DDR4 data control module 182 transmits the real-time cached data to the SATA interface module 162 according to the timing requirements of the SATA interface; the gigabit Ethernet communication module 19 includes a gigabit Ethernet transmission module 191 and a gigabit Ethernet reception module 192, and is connected to the clock module 11, the power supply module 12, the fiber optic Ethernet data reception module 131, the H.265 video encoding module 33, and the DDR4 data cache module 181. The gigabit Ethernet transmission module 191 receives the user instructions forwarded by the fiber optic Ethernet data reception module 131 and sends them to the H.265 video encoding module 33, and the gigabit Ethernet reception module 192 receives the encoded reaction video data transmitted by the H.265 video encoding module 33 and sends it to the DDR4 data cache module 181 for real-time high-speed caching.;

[0022] See Figure 1, the user service module 2 includes a user operation interface 21 and a user data storage module 22; the user operation interface 21 is connected to the fiber optic Ethernet data receiving module 131 and the user data storage module 22, and is used to generate user operation instructions and send them to the fiber optic Ethernet data receiving module 131, read reaction parameters and video data from the user data storage module 22 and display them to enhance user interaction; the data storage module 22 is connected to the fiber optic Ethernet data sending module 132, and is used to receive and store reaction parameters and video data.

[0023] Refer to Figure 1 , the video encoding module 3 includes an image sensor module 31, a MIPI interface module 32, and an H.265 video encoding module 33; the image sensor module 31 is connected to the MIPI interface module 32 and the flow chemistry device module 5, and is used to collect reaction video data in real time and transmit it to the MIPI interface module 32; the MIPI interface module 32 is connected to the image sensor module 31 and the H.265 video encoding module 33, and is used to transmit the reaction video data collected by the image sensor module 31 to the H.265 video encoding module 33; the H.265 video encoding module 33 is connected to the gigabit Ethernet receiving module 192 and the MIPI interface module 32, and is used to receive the unencoded reaction video data transmitted by the MIPI interface module 32, perform H.265 encoding, and output it to the gigabit Ethernet receiving module 192.

[0024] Refer to Figure 1, the industrial control board 4 includes an RS485 module 41, a PWM output module 42, a relay matrix module 43, an ADC sampling module 44, and a motor drive module 45; the RS485 module 41 is connected to the real-time parameter acquisition module 152, the control signal generation module 154, and the flow chemistry equipment module 5, receives the control signal sent by the control signal generation module 154 and precisely controls the relevant flow chemistry equipment, and at the same time transmits the feedback parameters of the relevant equipment to the real-time parameter acquisition module 152; the PWM output module 42 is connected to the control signal generation module 154 and the flow chemistry equipment module 5, receives the control signal and generates a corresponding PWM wave to control the relevant equipment in the flow chemistry equipment module 5; the relay matrix module 43 is connected to the control signal generation module 154 and the flow chemistry equipment module 5, receives the control signal and performs a switching operation on the relay matrix to control the relevant equipment in the flow chemistry equipment module 5; the ADC sampling module 44 is connected to the real-time parameter acquisition module 152, the control signal generation module 154, and the flow chemistry equipment module 5, receives the control signal and performs real-time sampling on each experimental parameter in the flow chemistry equipment module 5, and transmits the sampling data to the real-time parameter acquisition module 152; the motor drive module 45 is connected to the control signal generation module 154 and the flow chemistry equipment module 5, receives the control signal and generates a motor control signal to drive the motor in the flow chemistry equipment module 5 to work precisely.

[0025] Refer to Figure 2, after the present invention starts to work, first, the power-on system status detection module generates detection instructions for each device and sends them to the industrial control board. The industrial control board receives a series of detection instructions, sends corresponding instructions to each device to detect whether each device of the system is working properly. If any device is not in the normal working state, the user will be notified, and after troubleshooting, power on again. On the premise that all devices are working properly, the user enters the user operation interface to configure each flow chemistry experiment device, and can choose whether to store the video data of the reaction process. If the user chooses to store the reaction video data, the user operation interface will send a video encoding module start instruction and receive the encoded reaction video data output by the video encoding module in real time. The gigabit Ethernet interface receives the reaction video data and sends it to the DDR4 real-time cache, and stores the video data in the cache in the hard disk of the SATA interface. If the user chooses not to store the reaction video data, the operation of the user operation interface will be sent to the FPGA core control board through the fiber optic Ethernet communication module and transmitted to the control signal generation module. The control signal generation module receives the configuration instruction and generates relevant control signals to send to the industrial control board, and the industrial control board will control each flow chemistry experiment device according to each control signal. During the experiment, the system samples, stores, and analyzes each experiment parameter in real time. The FPGA core control board sends all experiment parameters and the experiment progress to the user interaction module through the fiber optic Ethernet communication module until the experiment is completed.

[0026] The present invention can support n reaction lines to conduct experiments simultaneously, and only one reaction line is used in the "traffic light reaction" experiment.

[0027] Refer to Figure 3 , taking the "traffic light reaction" experiment as an example, the flow chemistry device module 5 includes a photoelectric bubble sensor 51, a liquid storage bottle array 52, a vertical injection pump 53, an electromagnetic switching valve 54, a pressure sensor 55, a temperature control module 56, a flow chemical reaction chip 57, a temperature sensor 58, a back pressure valve 59, a pH sensor 510, a product liquid storage bottle 511, a mass sensor 512, and a waste liquid storage bottle 513.

[0028] Refer to Figure 3 , in the "traffic light reaction" experiment, for the electrical connection part, the photoelectric bubble sensor 51 is connected to the ADC sampling module 44; the vertical injection pump 53 is connected to the RS485 module 41; the electromagnetic switching valve 54 is connected to the relay matrix module 43; the pressure sensor 55 is connected to the RS485 module 41; the temperature control module 56 is connected to the PWM output module 42; the temperature sensor 58 is connected to the ADC sampling module 44; the back pressure valve 59 is connected to the motor drive module 43; the pH sensor 510 is connected to the ADC sampling module 44; the mass sensor 512 is connected to the ADC sampling module 44.

[0029] Refer to Figure 3 In the "traffic light reaction" experiment, for the non-electrically connected parts, the image sensor module 141 monitors the entire intelligent reaction platform; the image sensor module 31 captures the image of the flow chemical reaction chip 57 during the reaction process; the optoelectronic bubble sensor 51 contacts the liquid storage bottle array 52 to detect whether the reaction liquid therein is sufficient; the reaction liquid in the liquid storage bottle array 52 is sucked into the vertical injection pump 53 through the channel of the electromagnetic switching valve 54; the liquid in the vertical injection pump 53 is injected through the electromagnetic switching valve 54 into the pressure sensor 55 to detect the pressure value of the detection path; after flowing through the pressure sensor 55, the reaction liquid enters the flow chemical reaction chip 57; the flow chemical reaction chip 57 is placed on the temperature control module 56; the temperature sensor 58 is placed on the flow chemical reaction chip 57 to detect the reaction temperature; the liquid that has fully reacted inside the flow chemical reaction chip 57 flows through the pressure sensor 55; a back pressure valve 59 is connected to the rear stage of the pressure sensor 55 to control the pressure of the entire reaction system; a electromagnetic switching valve 54 is connected behind the back pressure valve 59 to distinguish the product and waste liquid paths; the product enters the product storage bottle 511 through the electromagnetic switching valve 54; the waste liquid enters the waste liquid storage bottle 513 through the electromagnetic switching valve 54; the product storage bottle 511 is placed on the mass sensor 512; the pH sensor is placed inside the product storage bottle 511.

[0030] Refer to Figure 3In the "traffic light reaction" experiment, all user operations are based on the user operation interface. After the system is powered on, it will automatically detect the system status to ensure that all devices are in normal working condition. If any device is not in normal working condition, the user operation interface will pop up a window to notify the user. After troubleshooting, power on again. After starting the experiment, the user first switches the first electromagnetic switching valve to the first fluid channel and operates the first vertical syringe pump to draw 5 mL of indigo disulfonate solution from the liquid storage bottle array at a flow rate of 5 mL / min; then switches the second electromagnetic switching valve to the first fluid channel and operates the second vertical syringe pump to draw 5 mL of alkaline glucose solution from the liquid storage bottle array at a flow rate of 5 mL / min; during the operation, the user can view the entire intelligent experimental platform screen to observe whether there are any emergencies such as liquid leakage. At the same time, the user can choose to save the video of the flow chemical reaction chip. The image sensor will collect, encode the video of the flow chemical reaction chip and transmit it to the FPGA core control board, and store it in the SATA hard disk. Next, the reaction temperature of the flow chemical reaction chip can be set to 60 °C. The temperature sensor will collect temperature data in real time and transmit it to the FPGA core control board. Once the detected temperature deviates from the set value, a control signal will be generated to drive the temperature control module to maintain a constant temperature. Then switch the first electromagnetic switching valve to the third fluid channel and operate the first vertical syringe pump to inject 5 mL of indigo disulfonate solution at a flow rate of 1 mL / min; then switch the second electromagnetic switching valve to the third fluid channel and operate the second vertical syringe pump to inject 5 mL of alkaline glucose solution at a flow rate of 1 mL / min. The two reactants are injected into the flow chemical reaction chip for full reaction; during the injection process, the real-time measured values of the three pressure sensors can be observed, and the pressure inside the reaction system can be adjusted to 80 kPa through the back pressure valve. During the experiment, it can be observed that the solution in the microfluidic chip will produce a green-red-yellow color change phenomenon with the change of time and space, and the pH sensor and mass sensor will detect the pH of the liquid in the product liquid storage bottle and the mass of the product in real time.

[0031] The above is only a further description of the present invention, and is not intended to limit the present invention. All equivalent implementations of the present invention should be included within the scope of the claims of the present invention. For those of ordinary skill in the art, equivalent substitutions or changes can be made according to the technical solutions and inventive concepts of the present invention, and all such changes or substitutions should fall within the protection scope of the appended claims of the present invention.

Claims

1. An intelligent microfluidic chemical reaction platform based on FPGA, characterized in that: The platform includes an FPGA core control board (1), a user service module (2), a video encoding module (3), an industrial control board (4) and a flow chemical equipment module (5); The FPGA core control board (1) is connected to the user service module (2), the video encoding module (3), the industrial control board (4) and the flow chemical equipment module (5), and is used to receive and analyze the instruction data issued by the user service module (2) and upload the experimental data collected in real time by the industrial control board (4) during the reaction process of the flow chemical equipment module (5), and receive the encoded flow chemical equipment module (5) experimental monitoring video output by the video encoding module (3); The user service module (2) is connected to the FPGA core control board (1) and is used to receive experimental data and monitoring video sent by the FPGA core control board (1), and send user operation instructions to the FPGA core control board (1); The video encoding module (3) is connected to the FPGA core control board (1) and the flow chemistry equipment module (5) to obtain monitoring video during the experiment of the flow chemistry equipment module (5) and transmit the video to the FPGA core control board (1) for storage after encoding it with H.265; The industrial control board (4) is connected to the FPGA core control board (1) and the flow chemical equipment module (5) to receive control instructions sent by the FPGA core control board (1), collect the values ​​of various experimental parameters in the reaction process of the flow chemical equipment module (5) in real time, and transmit them to the FPGA core control board (1) for processing; The flow chemistry equipment module (5) is connected to the FPGA core control board (1) and the industrial control board (4) to receive control signals sent by the industrial control board (4) and accurately execute the control signals. The reaction process image is collected in real time by the FPGA core control board (1).

2. The FPGA-based intelligent microfluidic chemical reaction platform according to claim 1, characterized in that: The FPGA core control board (1) comprises a clock module (11), a power module (12), an optical fiber Ethernet communication module (13), a MIPI image sensor module (14), a microfluidic chemical reaction control module (15), a video storage module (16), a video output module (17), a data cache module (18) and a Gigabit Ethernet communication module (19); The clock module (11) is connected to a power module (12), an optical fiber Ethernet communication module (13), a video monitoring module (14), a microfluidic chemical reaction control module (15), a video storage module (16), a video output module (17), a data cache module (18) and a Gigabit Ethernet communication module (19), and provides a reference clock for each module; The power module (12) is connected to the clock module (11), the optical fiber Ethernet communication module (13), the MIPI image sensor module (14), the microfluidic chemical reaction control module (15), the video storage module (16), the video output module (17), the data cache module (18) and the Gigabit Ethernet communication module (19) to supply power to each module; The optical fiber Ethernet communication module (13) comprises an optical fiber Ethernet data receiving module (131) and an optical fiber Ethernet data sending module (132), which are connected to the clock module (11), the power module (12), the microfluidic chemical reaction control module (15), the video output module (17), the Gigabit Ethernet communication module (19) and the user service module (2), and receives the operation instructions sent by the user service module (2) through the optical fiber Ethernet data receiving module (131), and transmits the operation instructions to the Gigabit Ethernet communication module (19) and the microfluidic chemical reaction control module (15); the optical fiber Ethernet data sending module (132) receives the video signal output by the video output module (17), the various experimental data collected by the microfluidic chemical reaction control module (15) and the judgment result of the experimental progress, and sends the above data in a TCP data packet format to the user service module (2); The video monitoring module (14) comprises a MIPI interface module (141) and an image sensor module (142), and is connected to a clock module (11), a power module (12), a flow chemistry equipment module (5) and a video output module (17); the image sensor module (142) collects images of the flow chemistry equipment module (5) during an experiment in real time and transmits the images to the video output module (17) through the MIPI interface module (141) for output and display, so as to be used for observation and demonstration of the experiment process; The microfluidic chemical reaction control module (15) comprises a reaction progress judgment module (151), a real-time parameter acquisition module (152), a power-on system state detection module (153) and a control signal generation module (154), and is connected to a clock module (11), a power module (12), an optical fiber Ethernet data receiving module (131), an optical fiber Ethernet data sending module (132) and an industrial control board (4). The reaction progress judgment module (151) judges the progress of the reaction according to the experimental process parameters collected by the real-time parameter acquisition module (152), and transmits the result to the optical fiber Ethernet data sending module (132); the real-time parameter acquisition module (152) directly transmits the collected experimental process parameters to the optical fiber Ethernet data sending module (132); the power-on system state detection module (153) generates initialization check instructions for each component of the flow chemical equipment module (5) when the system is powered on, and generates corresponding control signals through the control signal generation module (154) to perform a state check on the initially powered-on system to ensure that each component functions normally; The video storage module (16) comprises a SATA hard disk module (161) and a SATA interface module (162), and is connected to the clock module (11), the power module (12), the video output module (17) and the data cache module (18); the SATA interface module (162) receives the encoded reaction video data transmitted by the data cache module (18), stores the data in the SATA hard disk module (161), and sends the stored reaction video data to the video output module (17) for decoding and output; The video output module (17) comprises an H.265 video decoding module (171) and an HDMI interface module (172), and is connected to a clock module (11), a power module (12), an optical fiber Ethernet transmission module (132), a MIPI interface module (141) and a SATA interface module (162), allowing the reception of uncoded real-time monitoring video data transmitted by the MIPI interface module (141) and output through the HDMI interface module (172), and is also capable of receiving response video data stored in the SATA hard disk module (161) and transmitted by the SATA interface module (162), and transmitting the data to the HDMI interface module (172) or the optical fiber Ethernet data transmission module (132) after being decoded by the H.265 video decoding module (171); The data cache module (18) comprises a DDR4 data cache module (181) and a DDR4 data control module (182), and is connected to the clock module (11), the power module (12), the SATA interface module (162) and the Gigabit Ethernet communication module (19); the DDR4 data cache module (181) caches the encoded response video data transmitted from the Gigabit Ethernet communication module (19) in real time at high speed; and the DDR4 data control module (182) transmits the real-time cached data to the SATA interface module (162) according to the timing requirements of the SATA interface; The Gigabit Ethernet communication module (19) comprises a Gigabit Ethernet sending module (191) and a Gigabit Ethernet receiving module (192), which are connected to the clock module (11), the power module (12), the optical fiber Ethernet data receiving module (131), the H.265 video encoding module (33) and the DDR4 data cache module (181); the Gigabit Ethernet sending module (191) receives a user instruction forwarded by the optical fiber Ethernet data receiving module (131) and sends it to the H.265 video encoding module (33); the Gigabit Ethernet receiving module (192) receives the encoded response video data transmitted by the H.265 video encoding module (33) and sends it to the DDR4 data cache module (181) for real-time high-speed caching.

3. The FPGA-based intelligent microfluidic chemical reaction platform according to claim 1, characterized in that: The user service module (2) comprises a user operation interface (21) and a user data storage module (22); The user operation interface (21) is connected to the optical fiber Ethernet data receiving module (131) and the user data storage module (22) to generate user operation instructions and send them to the optical fiber Ethernet data receiving module (131), read reaction parameters and video data from the user data storage module (22) and display them, thereby enhancing user interaction; The data storage module (22) is connected to the optical fiber Ethernet data sending module (132) and is used to receive and store reaction parameters and video data.

4. The FPGA-based intelligent microfluidic chemical reaction platform according to claim 1, characterized in that: The video encoding module (3) comprises an image sensor module (31), a MIPI interface module (32) and an H.265 video encoding module (33); The image sensor module (31) is connected to the MIPI interface module (32) and the flow chemistry equipment module (5), collects reaction video data in real time and transmits it to the MIPI interface module (32); The MIPI interface module (32) is connected to the image sensor module (31) and the H.265 video encoding module (33), and transmits the reaction video data collected by the image sensor module (31) to the H.265 video encoding module (33); The H.265 video encoding module (33) is connected to the Gigabit Ethernet receiving module (192) and the MIPI interface module (32), receives unencoded response video data transmitted from the MIPI interface module (32), performs H.265 encoding, and then outputs the data to the Gigabit Ethernet receiving module (192).

5. The FPGA-based intelligent microfluidic chemical reaction platform according to claim 1, characterized in that: The industrial control board (4) comprises an RS485 module (41), a PWM output module (42), a relay matrix module (43), an ADC sampling module (44) and a motor drive module (45); The RS485 module (41) is connected to the real-time parameter acquisition module (152), the control signal generation module (154) and the flow chemical equipment module (5), receives the control signal sent by the control signal generation module (154) and accurately controls the relevant flow chemical equipment, and transmits the feedback parameters of the relevant equipment to the real-time parameter acquisition module (152); The PWM output module (42) is connected to the control signal generation module (154) and the flow chemical equipment module (5), receives the control signal and generates a corresponding PWM wave to control the relevant equipment in the flow chemical equipment module (5); The relay matrix module (43) is connected to the control signal generation module (154) and the flow chemical equipment module (5), receives the control signal and implements a switching operation on the relay matrix to control the relevant equipment in the flow chemical equipment module (5); The ADC sampling module (44) is connected to the real-time parameter acquisition module (152), the control signal generation module (154) and the flow chemical equipment module (5), receives the control signal and performs real-time sampling of various experimental parameters in the flow chemical equipment module (5), and transmits the sampled data to the real-time parameter acquisition module (152); The motor drive module (45) is connected to the control signal generation module (154) and the flow chemical equipment module (5), receives the control signal and generates a motor control signal to drive the motor in the flow chemical equipment module (5) to operate accurately.

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