Hair loss prevention composition

Abstract

The invention provides a hair loss prevention composition. The hair loss prevention composition comprises 0.03 to 0.1 weight percent of epigallocatechin gallyl glucoside; 0.2 to 1.5% by weight of soluble collagen; and a carrier acceptable in the personal care field. The invention also relates to an application of the composition in preparing a personal care product with an anti-hair-loss effect, wherein the personal care product is selected from an anti-hair-loss hair washing and care product and an eyelash plumping product.

Description

Technical Field

[0001] The present invention relates to the field of daily chemical care, and particularly to a hair anti - shedding composition with synergistic effect. Background Art

[0002] With the accelerating pace of people's lives, the increasing pressure in life and work, hair loss has become a common problem faced by the social population. In recent years, the problem of hair loss has also shown a trend of getting younger, and many people born in the 1980s or even 1990s have joined the ranks of hair loss. After investigation, most people with severe hair loss will choose specialist clinics and use therapeutic drugs, while consumers with less severe hair loss are more inclined to use anti - hair - loss hair care products in daily care to improve symptoms. According to statistics, among hair loss patients, the proportions of androgenetic alopecia and alopecia areata are relatively high. Anti - hair - loss hair care products are more helpful in solving the problem of seborrheic alopecia (androgenetic alopecia). Commonly used anti - hair - loss agents are mainly divided into two categories: plant - derived and non - plant - derived. Plant extracts with anti - hair - loss effects include: Platycladus orientalis extract, ginger extract, ginseng extract, polygonum multiflorum extract, eclipta prostrata extract, rhodiola rosea extract, angelica sinensis extract, ligusticum wallichii extract, mulberry leaf extract, etc. The anti - hair - loss mechanisms of different plant extracts are also different. Common anti - hair - loss active ingredients of non - plant origin include minoxidil, finasteride, adenosine, diaminopyrimidine oxide, pyrrolidinediaminopyrimidine oxide, etc. Due to their chemical origin, drug properties, or side effects, etc., their use in anti - hair - loss cosmetics is limited.

[0003] Therefore, the development of more safe, mild and effective bio - derived anti - hair - loss active ingredients is the need of the market.

[0004] Chinese Patent CN 118845516 A provides an anti - hair - loss composition and its application. The anti - hair - loss composition is composed of adenosine, coffee extract, L - carnitine tartrate, tripeptide - 1 copper, Platycladus orientalis extract and niacinamide. This composition has a significant synergistic effect in improving the antioxidant capacity of dermal papilla cells and inhibiting 5α - reductase. Through human efficacy tests, it can significantly reduce the number of shed hairs, significantly increase hair density, significantly increase the proportion of anagen hairs, significantly reduce the proportion of telogen hairs, and significantly increase the proportion of vellus hairs. However, the composition of this patent application is complex, with both chemical - derived and bio - derived components, which is not conducive to the later study of its mechanism of action.

[0005] Chinese Patent CN1640393 A discloses the application of tea polyphenols in drugs for preventing and treating hair loss caused by chemotherapy. The tea polyphenols referred to herein are extracts containing at least 30% epigallocatechin gallate (EGCG) with a flavanol structure extracted from tea leaves or the single component epigallocatechin gallate, and the test method is oral administration.

[0006] Chinese Patent CN 111991273 A A lotion with hair growth effect and its preparation method, characterized in that it contains 0.5%-10% by mass fraction of hair growth active substances, and the hair growth active substances are composed of ligusticum wallichii oil, glucosyl rutin, tocopherol nicotinate, epigallocatechin gallate glucoside, and diglucosyl gallic acid according to a mass ratio of 2-6:1:1:0.1-0.5:0.1-0.5.

[0007] However, so far, there has been no relevant report indicating that a composition composed of two active components, epigallocatechin gallate glucoside and soluble collagen, can promote hair follicle growth at a relatively low addition amount.

[0008] This application innovatively uses a combination of epigallocatechin gallate glucoside (INOVEOL EGCG) and soluble collagen to promote hair follicle growth and is used in the field of daily chemical care. Summary of the Invention

[0009] On the one hand, the present invention provides a hair loss prevention composition, which comprises:

[0010] 0.03-0.1% by weight of epigallocatechin gallate glucoside;

[0011] 0.2-1.5% by weight of soluble collagen; and

[0012] A carrier acceptable in the field of personal care.

[0013] In a preferred embodiment, the composition of the present invention contains 0.07-0.1% by weight of epigallocatechin gallate glucoside. In a preferred embodiment, the composition of the present invention contains 1.2-1.5% by weight of soluble collagen.

[0014] In a preferred embodiment, the hair loss prevention effect is achieved by promoting hair follicle growth. In a preferred embodiment, the hair loss prevention effect is achieved by increasing the content of VEGF.

[0015] On the other hand, the present invention also relates to the application of the composition in the preparation of a personal care product having a hair loss prevention effect.

[0016] In a preferred embodiment, the personal care product is selected from: anti-hair loss hair washing and hair care products and eyelash volumizing products. Brief Description of the Drawings

[0018] Figure 1 Shows photographs of vibrissa follicles taken every 7 days. Detailed Description of the Invention

[0019] The present invention relates to a natural - sourced composition having the efficacy of preventing hair loss. The composition is composed of soluble collagen and epigallocatechin gallate glucoside, which can promote the growth of hair follicles, increase the nutrients required for hair follicle growth, and can be used in anti - hair - loss hair washing and hair care products, eyelash volumizing products, etc.

[0020] The present invention unexpectedly discovers that the composition of epigallocatechin gallate glucoside and soluble collagen can promote the growth of ex vivo hair follicles, and the promoting effect of the combination of the two on hair follicle growth can be comparable to that of commercially available minoxidil solution, and even superior to commercially available minoxidil solution. The components in the composition are all of natural origin, which undoubtedly has great significance for expanding the market of mild and effective anti - hair - loss products with a gap, and can also inject new vitality into the eyelash volumizing market.

[0021] The object of the present invention is to provide a safe and effective hair anti - loss composition of natural origin, which is composed of a compound of epigallocatechin gallate glucoside and soluble collagen, providing new solutions for hair anti - loss and volumizing, and having potential application value in aspects such as preventing hair loss and eyelash volumizing.

[0022] For a more concise description, some quantitative expressions given herein are not modified by the term "about". It should be understood that whether or not the term "about" is explicitly used, each quantity given herein is intended to refer to the actual given value, and also intended to refer to the approximate value of these given values reasonably inferred by those of ordinary skill in the art, including the approximations of these given values caused by experimental and / or measurement conditions.

[0023] For a more concise description, some quantitative expressions herein are described as a range from about X amount to about Y amount. It should be understood that when a range is recited, the range is not limited to the recited upper and lower limits, but should include the entire range from about X amount to about Y amount or any amount therebetween.

[0024] Epigallocatechin gallate glucoside

[0025] Epigallocatechin gallate glucoside can be applied to the field of personal care products such as cosmetics, and is used as an antioxidant, skin protectant, etc. in cosmetics. However, there has been no report on using epigallocatechin gallate glucoside for hair anti - loss.

[0026] The composition of the present invention uses epigallocatechin gallate glucoside with the trade name INOVEOL EGCG purchased from Guangzhou Aoxue Chemical Co., Ltd.

[0027] In some embodiments, the composition of the present invention comprises about 0.03 - 0.1% by weight of epigallocatechin galloyl glucoside. In some embodiments, the composition of the present invention comprises about 0.05 - 0.1% by weight of epigallocatechin galloyl glucoside, preferably about 0.07 - 0.1% by weight of epigallocatechin galloyl glucoside.

[0028] Soluble collagen

[0029] Soluble collagen can be used to improve skin elasticity and firmness. Soluble collagen is a natural protein that can be absorbed by the skin and helps restore the elasticity and luster of the skin. However, there has been no report on using soluble collagen for preventing hair loss.

[0030] The composition of the present invention uses soluble collagen with the trade name Hair-Col17 purchased from Hangzhou Shiguang Xinya Biotechnology Co., Ltd.

[0031] In some embodiments, the composition of the present invention comprises about 0.2 - 1.5% by weight of soluble collagen. In some embodiments, the composition of the present invention comprises about 0.4 - 1.5% by weight of soluble collagen, preferably about 1 - 1.5% or about 1.2 - 1.5% by weight of soluble collagen.

[0032] Personal care products

[0033] The composition of soluble collagen and epigallocatechin galloyl glucoside can promote hair follicle growth, increase the nutrients required for hair follicle growth, and can be used in personal care products such as anti-hair loss hair washing and care products, eyelash volumizing products, etc.

[0034] The main object of the present invention is to provide a safe and effective hair loss prevention composition from natural sources, which is composed of a compound of epigallocatechin galloyl glucoside and soluble collagen, providing a new solution for hair loss prevention and hair volume increase. It has potential application value in preventing hair loss, eyelash volumizing, etc.

[0035] In some embodiments, the weight percentage of the composition of the present invention in the personal care product can be about 0.0001% - 20% (w / w), preferably the weight percentage is about 0.001% - 10% (w / w), more preferably the weight percentage is about 0.001% - 5% (w / w), and most preferably the weight percentage is about 0.01% - 5% (w / w).

[0036] The personal care product may also include one or more optional ingredients, which include, but are not exclusively limited to, pearlescent or opacifying agents, thickeners, auxiliary conditioning agents, humectants, chelating agents, and additives such as colorants, fragrances, preservatives, pH regulators, etc. that can improve its appearance, feel, and fragrance. For example, the pH of a shampoo product is preferably maintained at about 5 to about 7.5, more preferably about 5.5 to about 7.0.

[0037] Commercially available pearlescent or opacifying agents that can suspend water-insoluble additives such as silicones and / or enable the user to recognize that the product is a conditioning shampoo are suitable for the present invention. The amount of the pearlescent or opacifying agent used is about 1% to about 10% based on the total weight of the product, preferably about 1.5% to about 7%, more preferably about 2% to about 5%. Examples of suitable pearlescent or opacifying agents include, but are not limited to, (a) fatty acids having about 16 to about 22 carbon atoms and (b) mono- or diesters of ethylene glycol or propylene glycol; (a) fatty acids having about 16 to about 22 carbon atoms and (b) mono- or diesters of a polyalkylene glycol of the formula: HO-(JO) a -H, where J is an alkylene having about 2 to about 3 carbon atoms and a is 2 or 3; fatty alcohols having about 16 to about 22 carbon atoms; fatty acid esters of the formula: KCOOCH 2 L, where K and L each contain about 15 to about 21 carbon atoms; inorganic solids insoluble in the shampoo product, and mixtures thereof.

[0038] In a preferred embodiment, the pearlescent or opacifying agent is added to the shampoo product in the form of a preformed, stable aqueous dispersion, for example, commercially available from Henkel Corporation of Hoboken, New Jersey, under the trade name "Euperlan PK-3000". This substance is a combination of diethylene glycol distearate (the diester of ethylene glycol and stearic acid), lauryl ether-4 (CH 3 (CH 2 ) 10 CH 2 (OCH 2 CH 2 ) 4 OH), and cocamidopropyl betaine, and the weight percentages of the three combined are preferably about 25 to about 30: about 3 to about 15: about 20 to about 25, respectively.

[0039] Commercially available thickeners capable of imparting appropriate viscosity to the conditioning shampoo are applicable to the present invention. If a thickener is used, its amount in the shampoo product should be sufficient to increase the Brookfield viscosity value of the product to about 500 to about 10,000 centipoise. Examples of suitable thickeners include, but are not limited to: 1) polyethylene glycol of the formula: HO-(CH 2 CH 2 O) z H and 2) mono- or diesters of fatty acids containing about 16 to about 22 carbon atoms, where z is an integer from about 3 to about 200; ethoxylated polyol fatty acid esters; ethoxylated derivatives of mono- or diesters of fatty acids and glycerol; hydroxyalkyl celluloses; alkyl celluloses; hydroxyalkyl alkyl celluloses and mixtures thereof. Preferred thickeners include polyethylene glycol esters, more preferably PEG-150 distearate, which is commercially available from Stepan Company of Northfield, Illinois or from Comiel, S.p.A. of Bologna, Italy under the trade name "PEG 6000DS".

[0040] Commercially available auxiliary conditioning agents such as volatile silicones capable of imparting other properties to the hair such as luster are applicable to the present invention. If a volatile silicone conditioning agent is used, its boiling point at atmospheric pressure is preferably below about 220 °C. The amount of the volatile silicone conditioning agent is about 0% to about 3% based on the total weight of the product, such as about 0.25% to about 2.5% or about 0.5% to about 1.0%. Examples of suitable volatile silicones include, but are not limited to: polydimethylsiloxane, polydimethylcyclosiloxane, hexamethyldisiloxane, cyclomethicone fluids such as polydimethylcyclosiloxane commercially available from Dow Corning Corporation of Midland, Michigan under the trade name "DC-345" and mixtures thereof.

[0041] Commercially available humectants capable of imparting moisturizing and conditioning properties to personal care products are applicable to the present invention. The amount of the humectant is about 0% to about 10% based on the total weight of the product, preferably about 0.5% to about 5%, more preferably about 0.5% to about 3%. Examples of suitable humectants include, but are not limited to: 1) water-soluble liquid polyols selected from glycerol, propylene glycol, hexylene glycol, butylene glycol, dipropylene glycol and mixtures thereof; 2) polyalkylene glycol of the formula: HO-(R”O) b -H, where R” is an alkylene having about 2 to about 3 carbon atoms and b is an integer from about 2 to about 10; 3) of the formula: CH 3 -C 6 H 10 O 5- (OCH2 CH 2 ) c Polyethylene glycol ethers of methyl glucoside of -OH, where c is an integer from about 5 to about 25; 4) urea; and 5) mixtures thereof, glycerol being the preferred humectant.

[0042] Examples of suitable chelating agents include those that can protect and preserve the products of the present invention. The preferred chelating agent is EDTA, and more preferably disodium EDTA commercially available from Nouryon Chemicals Co., Ltd of Ningbo, China under the trade name "Dissolvine Na2", in an amount of from about 0% to about 0.5% based on the total weight of the product, preferably from about 0.05% to about 0.2%.

[0043] Suitable preservatives include sodium benzoate commercially available from Merck KGaA Darmstadt, German under the trade name " Sodium Benzoate", in an amount in the product of from about 0% to about 0.5% based on the total weight of the product, preferably from about 0.05% to about 0.45%.

[0044] Optionally, the personal care product may also contain at least one conditioning agent in an amount of from about 0.01% to about 1.0% based on the total weight, preferably from about 0.01% to about 0.5%, more preferably from about 0.01% to about 0.2%. Examples of suitable cationic conditioning agents include, but are not exclusive to, cationic cellulose derivatives; cationic guar gum derivatives; diallyldimethylammonium chloride and mixtures thereof.

[0045] The cationic cellulose derivative is preferably a polymeric quaternary ammonium salt obtained from the reaction of hydroxyethyl cellulose and a trimethylammonium-substituted epoxide. A substance called Polyquaternium-10, commercially available from Amerchol Corporation of Edison, New Jersey as "Polymer JR-400", is particularly useful in this regard.

[0046] The cationic guar gum derivative is preferably guar gum hydroxypropyltrimethylammonium chloride, commercially available from Rhodia of Cranbury, New Jersey under the trade name "Jaguar-17".

[0047] Other cationic conditioning polymers are those polymers derived from the monomer diallyldimethylammonium chloride. The homopolymer of this monomer is Polyquaternium-6, commercially available from Allied Colloids of Suffolk, Virginia, under the trade name "Salcare SC30". The copolymer of diallyldimethylammonium chloride and acrylamide is called Polyquaternium-7, also available from Allied Colloids, under the trade name "Salcare SC10".

[0048] In one embodiment, the conditioning agent portion comprises from about 0.01% to about 0.5%, such as from about 0.01% to about 0.2%, of a cationic guar derivative based on the total weight of the product and from about 0.01% to about 0.5%, such as from about 0.01% to about 0.2%, of a homopolymer or copolymer of diallyldimethylammonium chloride.

[0049] Examples

[0050] The present invention will be further described below in conjunction with specific examples. It is necessary to point out here that the examples are only used to further illustrate the present invention and should not be construed as limiting the scope of protection of the present invention. Those skilled in the art can make some non-essential improvements and adjustments based on the content of the present invention above. The test methods without specific conditions in the following examples are usually carried out under conventional conditions or according to the conditions recommended by the manufacturer. Unless otherwise specified, all percentages and parts are by weight.

[0051] The experimental materials used in the examples are as follows:

[0052] Epigallocatechin gallate glucoside, trade name: INOVEOL EGCG, purchased from Guangzhou Aoxue Chemical Co., Ltd., the raw material contains 95% (w / w) water and 5% (w / w) epigallocatechin gallate glucoside;

[0053] Soluble collagen, trade name: Hair-Col17, purchased from Hangzhou Shiguang Xinya Biotechnology Co., Ltd., the raw material contains 64.95% (w / w) water, 30% (w / w) butanediol, 5% (w / w) 1,2-hexanediol, and 0.05% (w / w) soluble collagen;

[0054] Positive control (PC): Minoxidil sulfate, purchased from Shanghai Yuanye Bio-Technology Co., Lot: N17GSS167999.

[0055] Test Example 1: HaCaT cell cytotoxicity test

[0056] 1 Basic principle: MTT colorimetric method

[0057] 2 Experimental procedures:

[0058] 2.1 Regular culture of HaCaT cells.

[0059] The cell source is Shanghai Jingkang Bioengineering Co., Ltd., and the culture medium is MEM with 10% FBS (fetal bovine serum). The sources of both FBS and MEM are Gibco.

[0060] 2.2 Cell seeding

[0061] Add 100 μL of culture medium to the outer wells (blank control) of a 96-well tissue culture plate, and add 100 μL of cell suspension with a density of 1×10 5 cells / mL (= 1×10 4 cells / well) to the remaining wells. The cells are cultured for 24 h until a confluent monolayer is formed. During this culture period, the cells recover vitality, attach, and grow exponentially.

[0062] 2.3 Addition of test substances

[0063] Remove the culture medium, and add 100 μL of samples with appropriate concentrations to each well. The cells are cultured for 24 h. The test substances are weighed samples, added to MEM with 10% FBS, and then diluted 7-fold at this concentration by a factor of 3.16.

[0064] 2.4 Measurement of MTT

[0065] The source of the MTT reagent is Sigma. Remove the culture medium, re-add 100 μL of culture medium, and add 20 μL of MTT stock solution. Incubate in an incubator for 2 - 4 h. Then remove the MTT solution, and add 100 μL of isopropanol to each well. Shake for 10 min, and measure the absorbance at 570 nm on an enzyme-linked immunosorbent assay (ELISA) reader.

[0066] 3 Data analysis

[0067] A decrease in the number of viable cells leads to a decrease in metabolic activity in the samples, which is directly related to the amount of blue-violet formazan produced. Measure the optical density at 570 nm. Calculate the survival rate by comparing with the blank equation according to the following formula:

[0068]

[0069] In the formula: OD 570a —Average optical density of the test sample; OD 570b —Average optical density of the blank.

[0070] When the survival rate is low, the potential cytotoxicity of the test sample is high.

[0071] The CV90 value (the concentration of the test sample at which 90% of the cells survive) obtained by fitting with GraphPad Prism software can be used as the maximum allowable addition concentration that is non-toxic to the cells.

[0072] 4 Experimental Results

[0073] Table 1: Cell survival rate data with soluble collagen as the test sample

[0074]

[0075]

[0076] Table 2: Cell survival rate data with epigallocatechin gallate glucoside as the test sample

[0077] Epigallocatechin gallate glucoside / % Survival rate (%) Control 100±3.15 10 9.74±2.82 3.1646 10.09±2.07 1.0014 12.36±1.33 0.3169 12.67±1.79 0.1003 90.58±3.56 0.0317 97.67±4.67 0.0100 97.76±4.45 0.0032 98.01±3.2

[0078] Table 3: CV90 values obtained by fitting with GraphPad Prism software

[0079] Raw material CV90 / % Soluble collagen 1.848％ Epigallocatechin gallate glucoside 0.1007％

[0080] Example 1:

[0081] A solution of 0.05% (w / w) soluble collagen, 0.01% (w / w) epigallocatechin gallate glucoside, and 99.94% (w / w) Williams E serum-free medium (purchased from Gibco) was mixed and dissolved evenly for testing.

[0082] Example 2:

[0083] A solution of 0.2% (w / w) soluble collagen, 0.03% (w / w) epigallocatechin gallate glucoside, and 99.77% (w / w) Williams E serum-free medium (purchased from Gibco) was mixed and dissolved evenly for testing.

[0084] Example 3:

[0085] A solution of 0.4% (w / w) soluble collagen, 0.05% (w / w) epigallocatechin gallate glucoside, and 99.55% (w / w) Williams E serum-free medium (purchased from Gibco) was mixed and dissolved evenly for testing.

[0086] Example 4:

[0087] A solution of 1% (w / w) soluble collagen, 0.07% (w / w) epigallocatechin gallate glucoside, and 98.93% (w / w) Williams E serum-free medium (purchased from Gibco) was mixed and dissolved evenly for testing.

[0088] Example 5:

[0089] A solution of 1.2% (w / w) soluble collagen, 0.1% (w / w) epigallocatechin gallate, and 98.7% (w / w) Williams E serum-free medium (purchased from Gibco) was mixed and dissolved evenly before testing.

[0090] Example 6:

[0091] A solution of 1.5% (w / w) soluble collagen, 0.1% (w / w) epigallocatechin gallate, and 98.4% (w / w) Williams E serum-free medium (purchased from Gibco) was mixed and dissolved evenly before testing.

[0092] Example 7:

[0093] A solution of 1.8% (w / w) soluble collagen, 0.1% (w / w) epigallocatechin gallate, and 98.1% (w / w) Williams E serum-free medium (purchased from Gibco) was mixed and dissolved evenly before testing.

[0094] Test Example 2: Hair Growth Test in Ex Vivo Hair Follicle Culture

[0095] 1 Hair follicle culture: Vibrissa follicles of 5-week-old SPF-grade C57BL / 6J healthy male mice (the mice were purchased from Guangdong Provincial Medical Laboratory Animal Center; production license number: SCXK(Yue)2022-0002; quality certificate number: 44007200143483) were incubated for 14 days at 37°C and 5% CO2, with 1 mL of Williams E serum-free medium (purchased from Gibco) in each well.

[0096] 2 Sample addition: Sample addition was performed on the next day of culture. The medium containing samples at different concentrations was added to the corresponding wells, and the medium was changed every 3 days.

[0097] 3 Determination of VEGF (vascular endothelial growth factor) content: After collecting the vibrissa follicles of mice on the 15th day of culture, the follicles in each group were transferred to cryogenic tubes, PBS was added, and they were kept in a -80°C refrigerator for standby. After the specimens were thawed, they were kept at 2 - 8°C, PBS was added, the follicles were ground manually, and the cells were broken by repeated freezing and thawing. After centrifugation, the supernatant was carefully collected, and the content of VEGF in the specimens was detected by ELISA.

[0098] 4 Image analysis: The length of the vibrissa follicles was photographed every 7 days, and the length of the vibrissa follicles was observed morphologically and measured. The hair follicle growth rate was calculated.

[0099] 5 Statistical analysis: IBM SPSS Statistics 20 software was used for significant statistical analysis.

[0100] 6 Experimental results

[0101] 6.1 Determination of the growth of the first batch of hair follicles and the content of VEGF in the hair follicles

[0102] Blank group: Pure Williams E serum-free medium solution

[0103] Positive control (PC): Commercially available minoxidil sulfate

[0104] 6.2 Experimental results

[0105] Figure 1 Pictures of the vibrissa hair follicles taken every 7 days are shown.

[0106] Table 4 shows the data of the growth of hair follicles and the content of VEGF in the hair follicles (MEAN±SD).

[0107] Table 4

[0108]

[0109]

[0110] Note: The meaning of the superscript letters - if there are no same letters in the superscripts of the data of two groups of samples, it means that the difference between the two groups of samples is significant; if there are same letters in the superscripts of the data, it means that the difference between the two groups of samples is not significant. For example, the superscripts of the hair follicle growth length data of Example 2 and Example 4 have the same letter b, so the difference between these two groups of samples is not significant; the superscripts of the hair follicle growth length data of Example 2 and the blank control group have no same letters, so the difference between these two groups of samples is significant.

[0111] As can be seen from Table 4, the growth rate, the growth length of the hair follicles, and the VEGF content in the hair follicles cultured with the test sample of Example 1 were all lower than those of the blank control group. The growth rate, the growth length, and the VEGF content in the hair follicles cultured with the test samples of Example 2, Example 3, Example 4, Example 5, and Example 6 were all higher than those of the blank control group. The growth rate and the growth length of the hair follicles cultured with the test sample of Example 7 were comparable to those of the blank control sample, while the VEGF content in the hair follicles was lower than that of the blank control group. The data indicate that when the addition amount of soluble collagen is in the range of 0.2% - 1.5% (w / w) and the addition amount of epigallocatechin gallate glucoside is in the range of 0.03% - 0.1% (w / w), the combination of the two has an effective promoting effect on both hair follicle growth and hair follicle nutrient supply, and can be used as a reference ingredient for the efficacy addition of natural-source anti-hair loss products. Example 1 (0.05% (w / w) soluble collagen + 0.01% (w / w) epigallocatechin gallate glucoside) had no promoting effect on hair follicle growth. The reason may be that when the concentration of the active substance is low, it will lead to the disorder of dermal papilla cell proliferation, thus affecting the normal development of hair follicles. Example 7 (1.8% (w / w) soluble collagen + 0.1% (w / w) epigallocatechin gallate glucoside) had an obvious inhibitory effect on hair follicle growth. Analyzing the reason, it may be that the addition concentrations of the two raw materials are both near their CV90 concentrations, and the cytotoxicity increases after superposition, thus having an inhibitory effect on hair follicle growth.

Claims

1. A hair loss prevention composition, comprising: 0.03-0.1 wt % of epigallocatechin galloside; 0.2-1.5 wt% soluble collagen; and A personal care acceptable carrier.

2. The composition according to claim 1, wherein The composition comprises 0.07-0.1 wt % of epigallocatechin galloside.

3. The composition according to claim 1, wherein The composition comprises 1.2-1.5% by weight of soluble collagen.

4. The composition according to claim 1, wherein The hair loss prevention effect is achieved by promoting the growth of hair follicles.

5. The composition according to claim 1, wherein The hair loss prevention effect is achieved by increasing the VEGF content.

6. Use of the composition according to any one of claims 1 to 5 in preparing a personal care product having a hair loss prevention effect.

7. The use according to claim 6, wherein: The hair loss prevention effect is achieved by promoting the growth of hair follicles.

8. The use according to claim 6, wherein: The hair loss prevention effect is achieved by increasing the VEGF content.

9. The use according to claim 6, wherein: The personal care product is selected from: anti-hair loss shampoo and hair care products and eyelash volumizing products.

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