Compositions for repair of skin photodamage and methods of making, using and cosmetic use thereof

Abstract

The application relates to the cosmetic technical field, and relates to a composition for repairing skin photo-damage and a preparation method, application and cosmetic thereof. The composition for repairing skin photo-damage comprises a Rehmannia glutinosa extract and a Angelica keiskei extract. In the composition for repairing skin photo-damage, the mass ratio of the Angelica keiskei extract to the Rehmannia glutinosa extract is (2-10):1. By compounding the Rehmannia glutinosa extract and the Angelica keiskei extract, a synergistic effect can be achieved within the above mass ratio range, the synergistic antioxidant effect is enhanced, the DPPH free radical and hydroxyl radical clearance rate of the composition is improved, and the repair effect of the composition is improved, so that the composition can be effectively used for repairing skin photo-damage.

Description

Technical Field

[0001] This application relates to the field of cosmetic technology, and more specifically, to a composition for repairing photodamage to the skin, its preparation method, application, and cosmetics. Background Technology

[0002] Photodamage to the skin occurs when excessive ultraviolet radiation causes oxidative stress in the body. This results in an overproduction of reactive oxygen species (ROS) or a slowed clearance of these substances, leading to a buildup of ROS and an imbalance in the body's oxidation and antioxidant systems, ultimately damaging the skin tissue.

[0003] The most direct effect of sun damage to the skin is gradual darkening. This is because ultraviolet radiation causes a large amount of melanin to deposit in the epidermis, and the marks are not easy to fade; over time, this accelerates skin aging.

[0004] Therefore, it is necessary to provide a new skin care product that can repair photodamage to the skin. Summary of the Invention

[0005] The purpose of this application is to provide a new composition for repairing photodamage to the skin, its preparation method, application, and cosmetics.

[0006] In a first aspect, this application provides a composition for repairing photodamage to the skin, the composition comprising: *Lysimachia christinae* extract and *Eriocaulon buergerianum* extract;

[0007] In the composition for repairing photodamage to the skin, the mass ratio of Ashitaba extract to Gynostemma pentaphyllum extract is (2-10):1.

[0008] In the above technical solution, by combining the extracts of *Lysimachia christinae* and *Lysimachia christinae* within the above-mentioned mass ratio range, a synergistic effect can be achieved, enhancing the synergistic antioxidant effect; increasing the DPPH and hydroxyl radical scavenging rate of the composition, and improving the repair effect of the composition, thus it can be effectively used for the repair of skin photodamage.

[0009] In other embodiments of this application, the composition for repairing photodamage to the skin further includes: Schizophyllum commune fermentation filtrate and polyols.

[0010] In other embodiments of this application, the composition for repairing photodamage to the skin, by weight percentage, comprises 20%–50% Ashitaba extract, 5%–10% Smilax glabra extract, 40%–50% Schizophyllum commune fermentation filtrate, and 10%–20% polyol.

[0011] In other embodiments of this application, the preparation method of the *Lysimachia christinae* extract includes the following steps:

[0012] The herb *Lysimachia christinae* was crushed to obtain herb powder, which was then extracted by soaking in water at 60℃~70℃.

[0013] In other embodiments of this application, the preparation method of Ashitaba extract includes the following steps:

[0014] Ashitaba leaves are pulverized to obtain Ashitaba powder, which is then added to an aqueous solution of polyol and extracted using high-pressure pulse extraction to obtain an extract. Optionally, the electric field of the high-pressure pulse extraction is 3 kV / cm to 4 kV / cm, and the number of pulses is 50 to 100.

[0015] In other embodiments of this application, the aqueous solution of the polyol is soaked in an alcohol solution with a mass percentage of 30% to 40%.

[0016] In other embodiments of this application, the polyol includes one or more of 1,3-propanediol or glycerol.

[0017] Secondly, this application provides a method for preparing a composition for repairing photodamage to the skin, comprising the following steps:

[0018] By mixing extracts of *Lysimachia christinae*, *Eriocaulon buergerianum*, fermentation filtrate of *Schizophyllum commune*, and polyols, a composition for repairing photodamage to the skin is obtained.

[0019] Thirdly, this application provides the use of a composition for repairing photodamage to the skin in the preparation of cosmetics.

[0020] Fourthly, this application provides a cosmetic product comprising the aforementioned composition for repairing photodamage to the skin.

[0021] In other embodiments of this application, the amount of the composition added to the cosmetic is greater than or equal to 0.05 wt%. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions in the embodiments of this application will be clearly and completely described below. Obviously, the described embodiments are some embodiments of this application, but not all embodiments.

[0023] Therefore, the following detailed description of the embodiments of this application is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments of the application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0024] This application provides a composition for repairing photodamage to the skin, comprising: *Euphorbia hirta* extract and *Euphorbia pekinensis* extract;

[0025] In the composition for repairing photodamage to the skin, the mass ratio of Ashitaba extract to Gynostemma pentaphyllum extract is (2-10):1.

[0026] In the above technical solution, by combining the extracts of *Lysimachia christinae* and *Lysimachia christinae* within the above-mentioned mass ratio range, a synergistic effect can be achieved, enhancing the synergistic antioxidant effect; increasing the DPPH and hydroxyl radical scavenging rate of the composition; and improving the repair effect of the composition, thereby effectively using it for the repair of photodamage to the skin.

[0027] Gynura divaricata (L.), also known as white-backed vegetable, white-seed vegetable, and chicken-feather vegetable, is a perennial herb mainly produced in Guangxi and distributed throughout southern provinces. The whole plant has heat-clearing and detoxifying properties, promotes blood circulation, removes blood stasis, dispels wind, and reduces swelling. The root is used medicinally, having the effects of purging fire, cooling blood, and promoting body fluid production. It is a common traditional Chinese medicine used to treat various inflammations such as mastitis, arthritis, and bronchitis.

[0028] Ashitaba (Angelica keiskei Koidz.), a perennial herb belonging to the Apiaceae family and the Angelica genus, is a plant used for both food and medicinal purposes. Ashitaba has a sweet, salty, and slightly bitter taste, and is cool in nature. It enters the spleen, kidney, and liver meridians, and has the effects of clearing turbidity, lowering lipids, and tonifying the liver and kidneys. Ashitaba contains various bioactive components, mainly used in the health food industry, such as chalcones, coumarins, and flavonoids, which have improving effects on hypertension, insomnia, gastrointestinal diseases, palpitations, arteriosclerosis, cirrhosis, and constipation.

[0029] The inventors were pleasantly surprised to discover that combining *Smilax glabra* extract and *Eriocaulon buergerianum* extract produces a synergistic effect, significantly enhancing antioxidant and cell repair properties. Applying this combination to cosmetics not only effectively improves their efficacy in repairing photodamage but also expands the application of *Smilax glabra* and *Eriocaulon buergerianum* in cosmetics. This has significant practical implications for the development of green, all-natural skincare products.

[0030] Further optionally, and exemplary, in some embodiments of this application, the mass ratio of Ashitaba extract to Echinopsis pilosula extract in the composition for repairing photodamage to the total skin is (2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, or any two of the aforementioned values): 1.

[0031] Furthermore, in some embodiments of this application, the composition for repairing photodamage to the skin further includes: Schizophyllum commune fermentation filtrate and polyol.

[0032] Furthermore, in some embodiments of this application, the composition for repairing photodamage to the skin, by weight percentage, includes 20%–50% Ashitaba extract, 5%–10% Smilax glabra extract, 40%–50% Schizophyllum commune fermentation filtrate, and 10%–20% polyol.

[0033] For example, in some embodiments of this application, the composition for repairing photodamage to the skin comprises, by weight percentage, 20%, 25%, 30%, 35%, 40%, 45%, 50%, or any two of the aforementioned values ​​of Ashitaba extract.

[0034] 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, or any range between two of the aforementioned values;

[0035] 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, or any two of the aforementioned values ​​in the fermentation filtrate of Schizophyllum commune;

[0036] Polyols of 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, or any two of the aforementioned values.

[0037] Furthermore, in some embodiments of this application, the aforementioned polyol includes one or more of glycerol and 1,3-propanediol.

[0038] For example, in some embodiments of this application, the polyol is either glycerol or 1,3-propanediol; or in some embodiments of this application, the polyol is a mixture of glycerol and 1,3-propanediol; the raw materials in the mixture can be mixed in any proportion.

[0039] Furthermore, in some embodiments of this application, the preparation of the *Schizophyllum commune* fermentation filtrate is as follows:

[0040] (1) Activation of Schizophyllum commune strain: The Schizophyllum commune strain was transferred to a plate medium for activation and cultured at 28°C for 5-7 days until the mycelium covered the plate; then the plate strain was transferred to a liquid medium and cultured for 3 days to obtain the fermentation seed liquid;

[0041] (2) Preparation of fermentation medium: The fermentation medium includes the following components: 0.3% yeast extract, 0.05% magnesium sulfate, 0.1% potassium dihydrogen phosphate, 3% glucose and the balance water, pH natural (about 6.0);

[0042] (3) Collect fermentation products: Inoculate the Schizophyllum commune seed liquid from step (1) into the sterilized culture medium above, and ferment at 28°C and 160 r / min for 48 hours to complete the fermentation. Filter to obtain Schizophyllum commune fermentation filtrate.

[0043] Furthermore, in some embodiments of this application, the preparation method of the *Lysimachia christinae* extract includes the following steps:

[0044] The herb *Lysimachia christinae* was crushed to obtain herb powder, which was then extracted by soaking in water at 60℃~70℃.

[0045] Exemplary, in some embodiments of this application, the preparation method of *Lysimachia christinae* extract includes the following steps:

[0046] The herb *Lysimachia christinae* was pulverized to obtain herb powder, which was then extracted by soaking in water at temperatures ranging from 60°C, 61°C, 62°C, 63°C, 64°C, 65°C, 66°C, 67°C, 68°C, 69°C, 70°C, or any two of the aforementioned values.

[0047] Furthermore, in some embodiments of this application, the method for preparing the Ashitaba extract includes the following steps:

[0048] Ashitaba leaves are pulverized to obtain Ashitaba powder, which is then added to an aqueous solution of polyol and extracted using high-pressure pulse extraction to obtain an extract. Optionally, the electric field of the high-pressure pulse extraction is 3 kV / cm to 4 kV / cm, and the number of pulses is 50 to 100.

[0049] Exemplary, in some embodiments of this application, the method for preparing Ashitaba extract includes the following steps:

[0050] Ashitaba leaves are pulverized to obtain Ashitaba powder, which is then added to an aqueous solution of polyol and extracted using high-pressure pulse extraction to obtain an extract. Optionally, the electric field for high-pressure pulse extraction is 3 kV / cm, 3.1 kV / cm, 3.2 kV / cm, 3.3 kV / cm, 3.4 kV / cm, 3.5 kV / cm, 3.6 kV / cm, 3.7 kV / cm, 3.8 kV / cm, 3.9 kV / cm, 4 kV / cm, or any two of the aforementioned values; the number of pulses is 50, 52, 55, 58, 60, 62, 65, 68, 70, 72, 75, 78, 80, 82, 85, 88, 90, 92, 95, 98, 100, or any two of the aforementioned values.

[0051] Furthermore, in some embodiments of this application, the aqueous solution of the polyol is soaked in an alcohol solution with a mass percentage of 30% to 40%.

[0052] For example, in some embodiments of this application, the aqueous solution of polyol is soaked in an alcohol solution with a mass percentage of 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, or any two of the aforementioned values.

[0053] Furthermore, in some embodiments of this application, the polyols mentioned above include one or more of 1,3-propanediol or glycerol.

[0054] For example, in some embodiments of this application, the polyol is 1,3-propanediol or glycerol; or in some embodiments of this application, the polyol is a mixture of 1,3-propanediol and glycerol, wherein the raw materials in the mixture can be mixed in any proportion.

[0055] This application provides a method for preparing a composition for repairing photodamage to the skin, comprising the following steps:

[0056] By mixing extracts of *Lysimachia christinae*, *Eriocaulon buergerianum*, fermentation filtrate of *Schizophyllum commune*, and polyols, a composition for repairing photodamage to the skin is obtained.

[0057] Some embodiments of this application provide the use of a composition for repairing photodamage to the skin in the preparation of cosmetics.

[0058] Some embodiments of this application provide a cosmetic product, including the skin photodamage repair composition provided in any of the foregoing embodiments;

[0059] Optionally, in some embodiments of this application, the amount of the above-described composition added to the cosmetic is greater than or equal to 0.05 wt%. Exemplarily, the amount of the above-described composition added to the cosmetic is 0.05 wt%, 0.06 wt%, 0.07 wt%, 0.08 wt%, 0.09 wt%, 0.1 wt%, 0.2 wt%, 0.3 wt%, 0.5 wt%, or a range between any two of the aforementioned values.

[0060] The features and performance of this application will be further described in detail below with reference to embodiments:

[0061] Example 1

[0062] A composition for repairing photodamage to the skin is provided, comprising: *Lysimachia christinae* extract and *Eriocaulon buergerianum* extract. The mass percentage of each ingredient in the composition is shown in Table 1.

[0063] The preparation process of the composition is as follows:

[0064] Step S1: Crush the dried *Lysimachia christinae* (a type of herb) into powder, sieve it, and obtain *Lysimachia christinae* powder. Add 100g of water to 10g of *Lysimachia christinae* powder, soak and extract at 70℃ for 1 hour, filter, and obtain *Lysimachia christinae* extract.

[0065] Step S2: Crush the dried Ashitaba leaves and sieve them to obtain Ashitaba powder. Add 100g of a 40% (w / w) glycerol aqueous solution to 10g of Ashitaba powder and stir until homogeneous. Extract using high-pressure pulse extraction at an extraction field strength of 4kV / cm and a frequency of 100 times. Filter the mixture to obtain Ashitaba extract.

[0066] Step S3: Mix the *Euphorbia hirta* extract obtained in step S1) and the *Euphorbia esculenta* extract obtained in step S2); control the mass ratio of *Euphorbia hirta* extract to *Euphorbia esculenta* extract to be 1:9.

[0067] Example 2-3

[0068] A composition is provided that differs from Example 1 in the mass percentage of the composition (see Table 1 for details).

[0069] Examples 4-6

[0070] A composition is provided, which differs from Example 1 in that it also contains Schizophyllum commune fermentation filtrate and polyols. The mass percentages of each component are detailed in Table 1. Other preparation processes are the same as those in Example 1.

[0071] Comparative Example 1

[0072] A *Lysimachia christinae* extract for repairing photodamage to the skin is provided, and its preparation process is as follows;

[0073] The dried *Lysimachia christinae* was pulverized and sieved to obtain *Lysimachia christinae* powder. 10g of water was added to 10g of *Lysimachia christinae* powder, and the mixture was soaked and extracted at 70℃ for 1 hour. The extract was then filtered to obtain *Lysimachia christinae* extract.

[0074] (See Table 1 for details.)

[0075] Comparative Example 2

[0076] An extract of Ashitaba for repairing photodamage to the skin is provided, and the preparation process is as follows;

[0077] The dried Ashitaba leaves were pulverized and sieved to obtain Ashitaba powder. 100g of a 40% (w / w) glycerol aqueous solution was added to 10g of Ashitaba powder and stirred until homogeneous. Extraction was performed using high-pressure pulse extraction at an extraction field strength of 4kV / cm and a frequency of 100 pulses. The mixture was then filtered to obtain the Ashitaba extract. (See Table 1 for details).

[0078] Example 7

[0079] A composition for repairing photodamage to the skin is provided, comprising, by weight percentage:

[0080] 20% Ashitaba extract, 10% Gynostemma pentaphyllum extract, 60% glycerol, balance water. The preparation process is the same as in Example 1 above (see Table 1 for details).

[0081] Example 8

[0082] A composition for repairing photodamage to the skin is provided, comprising, by weight percentage:

[0083] The mixture consisted of 20% Ashitaba extract, 10% Gynostemma pentaphyllum extract, 60% Schizophyllum commune fermentation filtrate, and the remainder was water. The preparation process was the same as that in Example 1 above (see Table 1 for details).

[0084] Table 1. Composition ratios of the compositions

[0085]

[0086] [Performance Testing]

[0087] 1. Antioxidant properties

[0088] (1) The DPPH clearance rate test method is as follows:

[0089] DPPH, also known as 1,1-diphenyl-2-trinitrophenylhydrazine, is a very stable nitrogen-centered free radical. Its stability primarily stems from the steric hindrance of the three benzene rings through resonance stabilization, preventing the unpaired electrons on the central nitrogen atom from performing their proper electron pairing function. Its anhydrous ethanol solution is purple, exhibiting maximum absorption at 517 nm, with absorbance showing a linear relationship with concentration. Adding free radical scavengers can bind to or replace DPPH, reducing the number of free radicals, decreasing absorbance, and lightening the solution color, thus allowing evaluation of its free radical scavenging ability.

[0090] Prepare a 0.2 mmol / L DPPH methanol (or anhydrous ethanol) solution; prepare test sample solutions of different concentrations.

[0091] Pipette 2 mL of the sample solution and 2 mL of DPPH solution into stoppered test tubes, mix well, and react in the dark for 30 min. Measure the absorbance at 517 nm (A1). Pipette 2 mL of the sample solution and 2 mL of methanol into stoppered test tubes, mix well, and react in the dark for 30 min. Measure the absorbance at 517 nm (A2). Pipette 2 mL of DPPH solution and 2 mL of methanol into stoppered test tubes, mix well, and react in the dark for 30 min. Measure the absorbance at 517 nm (A0). Perform three replicates for each sample.

[0092]

[0093] (2) The test method for hydroxyl radicals is as follows:

[0094] The hydroxyl radicals generated by the Fenton reaction oxidize salicylic acid to produce 2,3-dihydroxybenzoic acid. The content of hydroxyl radicals is estimated by measuring the absorbance at 510 nm.

[0095]

[0096] The meanings of each letter are as follows:

[0097] A0: Absorbance of the control tube

[0098] A1: Absorbance of blank tube

[0099] A2: Absorbance of the measuring tube

[0100] The antioxidant properties of different concentrations of *Euphorbia hirta* extract are shown in Table 2; the antioxidant properties of different concentrations of *Euphorbia pekinensis* extract are shown in Table 3; and the antioxidant properties of the composition with a concentration of 0.05 wt% are shown in Table 4.

[0101] Table 2. Antioxidant performance test of *Lysimachia christinae* extract.

[0102] Concentration of *Lysimachia christinae* extract DPPH free radical scavenging rate Hydroxyl radical scavenging rate 0.01％ 98.11％ 36.06％ 0.00500％ 73.57％ 20.72％ 0.00250％ 53.07％ 9.22％ 0.00125％ 35.99％ 4.38％ 0.00063％ 29.17％ 1.47％

[0103] Table 3. Antioxidant properties of Ashitaba extract

[0104] Concentration of Ashitaba extract DPPH free radical scavenging rate Hydroxyl radical scavenging rate 0.050％ 53.41％ 72.48％ 0.025％ 52.37％ 71.59％ 0.013％ 42.66％ 49.10％ 0.006％ 28.06％ 16.78％ 0.003％ 22.65％ 12.34％ 0.002％ 20.47％ 8.89％

[0105] The data from the two tables above show that: 0.01% *Eupatorium fortunei* extract has a significant scavenging effect on DPPH free radicals, with a scavenging rate as high as 98%. Furthermore, within the concentration range of 0.00063%-0.01%, the scavenging effect shows a stoichiometric relationship with the concentration of *Eupatorium fortunei* extract; however, its scavenging effect on hydroxyl free radicals is relatively poor. Similarly, 0.025% *Akebia quinata* extract has a significant scavenging effect on hydroxyl free radicals, with a scavenging rate of 71.59%. Within the concentration range of 0.002%-0.050%, the scavenging effect shows a stoichiometric relationship with the concentration of *Akebia quinata* extract; however, its scavenging effect on DPPH free radicals is relatively weak.

[0106] Table 4 Antioxidant properties of the 0.05 wt% composition

[0107]

[0108] As can be seen from the table data above, the antioxidant performance of each embodiment is superior to that of each comparative embodiment. This is because:

[0109] Compared with Comparative Example 1, each embodiment of the present invention combined with Ashitaba extract showed better antioxidant activity compared with Ashitaba extract alone.

[0110] Compared with Comparative Example 2, each of the embodiments combined with the extract of *Eriocaulon buergerianum* showed better antioxidant activity compared with the extract of *Eriocaulon buergerianum* alone.

[0111] Furthermore, as can be seen from the table above, when the extract of *Lysimachia christinae* is combined with the extract of *Ephedra sinica*, the antioxidant effect of the composition can be enhanced synergistically in terms of both DPPH free radical scavenging rate and hydroxyl free radical scavenging rate.

[0112] Furthermore, when the concentration of *Euphorbia pekinensis* extract is within the range of 0.00063%-0.01%, and the concentration of *Euphorbia pekinensis* extract is within the range of 0.002%-0.050%, the antioxidant properties of the composition can be further enhanced, thereby improving the skin photodamage repair efficacy of the cosmetic. Therefore, when the composition concentration is 0.05wt%, it exhibits good antioxidant properties; thus, adding ≥0.05wt% of the composition to cosmetics can further enhance the skin photodamage repair efficacy of the cosmetic.

[0113] 2. UV radiation resistance test

[0114] (1) Select human fibroblasts in good viability, collect cells for counting, and prepare 2×10⁻⁶ cells. 5 Cell suspension of cells / mL, at 2 × 10⁶ cells / mL per well 4 Cells were seeded in 96-well plates, divided into groups, and incubated in a constant temperature incubator for 24 hours. The old culture medium was removed, and the cells were washed twice with PBS. Except for the control group, the remaining cells were treated with a radiation dose of 140 mJ / cm². 2 After UVB irradiation, 100 μL of fresh serum-containing culture medium was added to the blank group and the UVB group, while 100 μL of the UV damage repair composition prepared in each example or comparative example was added to the drug-treated group. The cells were incubated overnight in a constant temperature incubator, and cell growth was carefully observed under a microscope. 100 μL of 10% CCK-8 was added to each well of a 96-well plate, and the plates were incubated for 30 min. The absorbance was measured at 450 nm using a microplate reader.

[0115] Cell viability was calculated using a formula based on the CCK-8 assay.

[0116] Cell viability = [(experimental wells - blank wells) / (negative control wells - blank wells)] × 100%

[0117] The test results are shown in Table 5 below.

[0118] Table 5. Results of cell viability assay

[0119]

[0120]

[0121] (2) Cell scratch test

[0122] Select healthy human fibroblasts, collect cells for counting, and prepare 5×10⁶ cells. 5 A cell suspension of 1 cell / mL was added to 2mL of complete culture medium to allow cells to adhere and grow. The next day, when the cell confluence was approximately 95%, a vertical line was drawn along the marker's black line using a 10μL pipette tip. The original complete culture medium was discarded, and the cells were washed 2-3 times with PBS to remove cell debris. 1% of the UV damage repair composition from Example 5 and 10% serum were added to the culture medium. Cells were observed under an inverted microscope at 0h and 24h, and images were taken. The images were analyzed using ImageJ software to calculate the cell scratch migration rate.

[0123] Cell scratch migration rate = (mean initial inter-cell distance - mean inter-cell distance at time t) / mean initial inter-cell distance

[0124] The test results are shown in Table 6 below:

[0125] Table 6. Results of cell scratch migration rate detection

[0126]

[0127]

[0128] The data in the table above shows that:

[0129] The compositions provided in the various embodiments have higher cell survival rates than the combined samples provided in the UV model group and Comparative Examples 1-2, which demonstrates that the compositions provided in the embodiments of this application can effectively resist UV damage to cells.

[0130] The compositions provided in Examples 1-3 showed higher cell scratch migration rates than the blank control group and the combined samples provided in Comparative Examples 1-2; the compositions provided in Examples 4-6 and 7-8 showed higher cell scratch migration rates than the samples provided in the blank control group. This demonstrates that the compositions provided in the examples of this application can effectively improve the repair efficacy.

[0131] In summary, the composition provided in this application can effectively enhance the antioxidant and repair effects of the composition, and when applied to cosmetics, it can be effectively used for the repair of photodamage to the skin.

[0132] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A composition for repairing photodamage to the skin, characterized in that, The composition for repairing photodamage to the skin includes: *Lysimachia christinae* extract and *Akebia quinata* extract; In the composition for repairing photodamage to the skin, the mass ratio of the Ashitaba extract to the Smilax glabra extract is (2-10):1; The preparation method of the *Lysimachia christinae* extract includes the following steps: The herb *Lysimachia christinae* was crushed to obtain herb powder, which was then extracted by soaking in water at 60℃~70℃. The preparation method of the Ashitaba extract includes the following steps: Ashitaba leaves were pulverized to obtain Ashitaba powder, which was then added to an aqueous solution of polyol and extracted using a high-pressure pulse extraction method to obtain an extract. The electric field of the high-pressure pulse extraction was 3 kV / cm ~ 4 kV / cm, and the number of pulses was 50 ~ 100.

2. The composition for repairing photodamage to the skin according to claim 1, characterized in that, The composition for repairing photodamage to the skin also includes: Schizophyllum commune fermentation filtrate and polyols.

3. The composition for repairing photodamage to the skin according to claim 2, characterized in that, The composition for repairing photodamage to the skin, by weight percentage, comprises 20% to 50% of the Ashitaba extract, 5% to 10% of the Smilax china extract, 40% to 50% of the Schizophyllum commune fermentation filtrate, and 10% to 20% of the polyol.

4. The composition for repairing photodamage to the skin according to claim 1, characterized in that, The aqueous solution of the polyol is an alcohol solution with a mass percentage of 30% to 40%.

5. The composition for repairing photodamage to the skin according to claim 4, characterized in that, The polyols include one or more of 1,3-propanediol or glycerol.

6. A method for preparing a composition for repairing photodamage to the skin according to any one of claims 1-5, characterized in that, Includes the following steps: By mixing extracts of *Lysimachia christinae*, *Eriocaulon buergerianum*, fermentation filtrate of *Schizophyllum commune*, and polyols, a composition for repairing photodamage to the skin is obtained.

7. The use of a composition for repairing photodamage to the skin according to any one of claims 1-5 in the preparation of cosmetics.

8. A cosmetic product, characterized in that, The composition for repairing photodamage to the skin according to any one of claims 1-5 is included; the amount of the composition added to the cosmetic is greater than or equal to 0.05 wt%.

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