Rapid cutting propagation method for acer truncatum

Abstract

The invention belongs to the field of plant rapid propagation, and particularly relates to an acer truncatum cuttage rapid propagation method. According to the method, the optimal growth conditions for the acer truncatum of different tree ages are obtained by regulating and controlling the influence of the types, the concentrations and the growth matrixes of the cutting growth hormones of the acer truncatum of different tree ages, the optimal rooting combination of the acer truncatum big tree is that the current-year new branches of the big tree are treated by using perlite as a matrix and 200mg / L IBA, and the rooting rate reaches 56.67%. The optimal rooting combination of the ancient acer truncatum trees is that 200mg / L ABT is used for treating current-year new branches of the ancient trees, and the rooting rate reaches 24.67%. Research results show that acer truncatum of different tree ages needs to be treated by different hormones for rooting, so that technical support is provided for rapid propagation of acer truncatum of different tree ages. Compared with the prior art, the rooting rate of the acer truncatum is increased.

Description

Technical Field

[0001] The invention belongs to the field of plant rapid propagation, and particularly relates to a method for rapid propagation of Acer truncatum by cuttings. Background Art

[0002] Acer truncatum Bunge is a deciduous tree of the genus Acer L. in the family Sapindaceae Juss. It is named after its winged fruit, which resembles ancient Chinese gold ingots. Its leaf color has important ornamental value and significant garden and ecological value. Acer truncatum Bunge seeds are large in size and high in oil content. They contain hundreds of bioactive ingredients, including oleic acid and linoleic acid, which are as high as 53%, and the oil is of excellent quality. Its nucleoprotein content is 25%-27%, and it is known as a new resource of high-quality woody oil and food protein. The content of neuraminic acid in the seed oil is as high as 5-6%, which has the potential to treat Alzheimer's disease, memory loss, insomnia and amnesia, and can be developed into a health product. In addition, Acer truncatum Bunge is an important source of wood for high-end furniture.

[0003] In recent years, with the deepening of the study of Acer truncatum, scholars have collected, preserved, processed and utilized Acer truncatum as well as studied its molecular biology. At present, the breeding of Acer truncatum mainly relies on seed germination, so there are some problems such as uneven emergence of seedlings, recombination mutation, and loss of excellent traits of the mother tree. These problems have seriously restricted the development of the Acer truncatum industry. Most of the Acer truncatum with excellent traits in nature are large trees or ancient trees, so the asexual reproduction of large trees and ancient trees has become the key to restricting the development of the industry. Most scholars have conducted research on the rooting of Acer truncatum ... Some scholars have conducted research on the asexual reproduction of Acer truncatum. Qiao Qian and others obtained excellent asexual lines of Acer truncatum through grafting, and some scholars obtained asexual lines of ancient Acer truncatum trees through tissue culture.

[0004] The above studies found that the type, concentration and matrix of growth regulators all have an impact on the rooting of Acer truncatum. Rooting cuttings through methods such as sand storage is not only time-consuming and labor-intensive, but also has a long seedling raising cycle, is prone to mildew, and is difficult to guarantee the rooting rate of cuttings. Secondly, the operation is complicated and the actual production feasibility is not good. Therefore, there are still difficulties in factory-based seedling raising of large and ancient Acer truncatum trees, and the rooting method and rooting rate of Acer truncatum cuttings are still problems that need to be solved urgently. In response to these problems, this study used large and ancient Acer truncatum trees as materials to explore suitable rooting methods to solve the problem of seedling raising of large and ancient Acer truncatum trees. Summary of the invention

[0005] Based on the above purpose, the technical solution of the present application provides a method for rapid propagation of Acer truncatum by cuttings, comprising: soaking the Acer truncatum scion in growth hormone and then cutting it into a cutting medium, controlling the humidity and temperature and cultivating it under natural light.

[0006] Furthermore, the Acer truncatum scion is a healthy young branch without diseases and insect pests that sprouts in the current year, and is obtained by retaining one bud and one leaf.

[0007] Furthermore, the cutting matrix is ​​perlite.

[0008] Furthermore, when the Acer truncatum scion is taken from a large Acer truncatum tree, the growth hormone is IBA, and the concentration is 100-300 mg / L; when the Acer truncatum scion is taken from an ancient Acer truncatum tree, the growth hormone is ABT, and the concentration is 50-500 mg / L.

[0009] Furthermore, the IBA concentration is 200 mg / L, and the ABT concentration is 100 mg / L.

[0010] Furthermore, the humidity is maintained above 85% and the temperature is maintained at 25-30°C.

[0011] The beneficial effects of the present invention are as follows: the present application obtains the best growth conditions for Acer truncatum of different ages by regulating the types, concentrations and growth substrates of growth hormones for cuttings of Acer truncatum of different ages. Among them, the best combination for rooting of Acer truncatum trees is perlite as the substrate and 200 mg / L IBA to treat the new branches of the trees in the current year, and the rooting rate reaches 56.67%. The best combination for rooting of ancient Acer truncatum trees is perlite as the substrate and 200 mg / L ABT to treat the new branches of the ancient trees in the current year, and the rooting rate reaches 24.67%. The research results show that different hormone treatments are required for rooting of Acer truncatum of different ages, which provides technical support for the rapid propagation of Acer truncatum of different ages. Compared with the existing technology, the rooting rate of Acer truncatum is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 This is the picture of the Acer truncatum scion after processing;

[0013] Figure 2 This is a picture of the Acer truncatum scion after cutting;

[0014] Figure 3 This is a graph showing the effects of different exogenous hormones and concentrations on the rooting of Acer truncatum cuttings;

[0015] Figure 4 This is a graph showing the effects of different exogenous hormones and concentrations on the rooting of ancient Acer truncatum cuttings;

[0016] Figure 5 The following is a diagram of the morphological changes in the rooting process of Acer truncatum cuttings, where A indicates that the base begins to swell 10 days after cutting; B indicates that adventitious roots begin to sprout at the base 20 days after cutting; and C indicates that complete adventitious roots are formed 20 days after cutting. DETAILED DESCRIPTION

[0017] The specific implementation modes of the present invention are described below so that those skilled in the art can understand the present invention. However, it should be clear that the present invention is not limited to the scope of the specific implementation modes. For those of ordinary skill in the art, as long as various changes are within the spirit and scope of the present invention as defined and determined by the attached claims, these changes are obvious, and all inventions and creations utilizing the concept of the present invention are protected.

[0018] In the present application, the term "Acer truncatum tree" refers to an Acer truncatum tree with an age of 50±5 years.

[0019] The term "ancient Acer truncatum tree" refers to a Acer truncatum tree with an age of 100±10 years.

[0020] The term "IBA" means indolebutyric acid.

[0021] The term "ABT" refers to a broad-spectrum, highly effective compound plant growth regulator, namely ABT rooting powder.

[0022] The above-mentioned IBA and ABT can be purchased through commercial channels. The IBA and ABT used in this application are from Solebau.

[0023] Example 1 Acer truncatum scion cuttings

[0024] 1. Preparation of cutting materials: The scions of Acer truncatum large and ancient trees are taken from the Acer truncatum large and ancient trees in the National Long-term Scientific Research Base of Warm Temperate Forests in Jiulongshan, Beijing (39.96N, 116.09E), with tree ages of 50±5 years and 100±10 years respectively. They are healthy and pest-free tender branches that sprouted that year. The cuttings are 8 to 10 cm long, with one bud and a leaf (the leaf is trimmed into a circle with a diameter of about 10 cm), and the incision is flat on the top and oblique on the bottom (such as Figure 1 As shown in the figure, the experiment was carried out in April 2024 in the greenhouse of the North China Forestry Experimental Center of the Chinese Academy of Forestry in Mentougou District, Beijing. The greenhouse is a glass greenhouse covered with a sunshade net on the top, with automatic spray moisturizing, good water supply and drainage systems, and the air humidity is maintained above 85%. The temperature is 25-35℃.

[0025] 2. Cutting experiment

[0026] (1) Experiments with different cutting media

[0027] Use 65*45*20cm (length*width*height) as the cutting pool, which is convenient for ventilation and drainage. The cutting medium is perlite, vermiculite, and vermiculite: nutrient soil (1:1); spread the prepared medium in the cutting pool, about 18cm thick, and water it thoroughly with tap water before cutting.

[0028] (2) Experiments with different growth matrix types and concentrations

[0029] IBA and ABT were prepared in different concentrations (0, 50 mg / L, 100 mg / L, 200 mg / L, 500 mg / L) to soak the base of the cuttings, which were: CK (0), IBA50 (50 mg / L), IBA100 (100 mg / L), IBA200 (200 mg / L), IBA500 (500 mg / L), ABT50 (50 mg / L), ABT100 (100 mg / L), ABT200 (200 mg / L), ABT500 (500 mg / L).

[0030] The soaking time was 20 min, and each treatment was repeated 3 times, with 50 scions in each repetition. The rooting situation was counted 30 days after cuttings, and the rooting rate = the number of adventitious roots formed / the total number of cuttings (50)*100%.

[0031] 3. Cutting and post-management

[0032] like Figure 2 As shown, the pretreated cuttings are inserted into the spare matrix, then watered thoroughly with tap water, and the matrix around the cuttings is compacted; automatic spray moisturizing is used for the cuttings, and the setting time is 7:00-19:00, spraying once every half an hour, each time for 2 minutes, and once every hour for the rest of the time, the air humidity is maintained above 85%, the temperature is maintained at 25-30℃, and natural light is provided.

[0033] 4. Experimental Results

[0034] (1) Effects of different substrates on rooting of Acer truncatum cuttings

[0035] The large and ancient trees of Acer truncatum were cut into different substrates (perlite, vermiculite, and vermiculite: nutrient soil (1:1)). Due to the temperature and humidity inside the greenhouse, vermiculite and vermiculite: nutrient soil (1:1) caused a large amount of mold to grow about 10 days after the scion was cut, and the scion died. Therefore, perlite was determined to be the most suitable cutting substrate for Acer truncatum. Subsequent experimental studies used perlite as the substrate for the rooting study of Acer truncatum cuttings.

[0036] (2) Effects of different exogenous hormones and their concentrations on rooting of Acer truncatum cuttings

[0037] like Figure 3The young branches of Acer truncatum (50±5) were used as cutting materials. The effects of different exogenous hormone types and concentrations on the rooting rate of cuttings were studied, showing obvious differences ( Figure 3 ). Comparison showed that IBA200 and ABT100 had the highest rooting rates, especially IBA200, which had a rooting rate of 56.67%. The above results showed that IBA200 was the most suitable for rooting induction of Acer truncatum cuttings.

[0038] like Figure 4 The figure shows the factors affecting rooting of cuttings of old Acer truncatum trees. Different concentrations of IBA and ABT were used as exogenous hormones to induce rooting of tender branches of old Acer truncatum trees (100±10) in the same year. The study found that unlike the old Acer truncatum trees, the rooting rate of cuttings of Acer truncatum was significantly lower, and ABT was significantly better than IBA; in particular, ABT200 had the highest rooting rate, which could reach 24.67% ( Figure 4 The above results show that the age of Acer truncatum has a great influence on the rooting rate. As the age of Acer truncatum increases, its rooting rate decreases.

[0039] The above results show that the best growth hormone for large Acer truncatum trees is IBA200, and the best growth hormone for old Acer truncatum trees is ABT100.

[0040] Finally, the present application also discloses the morphological changes in the rooting process of Acer truncatum cuttings, such as Figure 5 As shown in Figure 1, it takes about 30 days for the new branches of Acer truncatum to take root. Ten days after cutting, the base of the cuttings begins to swell and grow callus tissue ( Figure 5 A); 20 days after cutting, white adventitious roots appeared near the cut at the base of the cuttings ( Figure 5 B); 30 days after cutting, the number of adventitious roots continued to increase and the color began to darken ( Figure 5 C); The rooting rate no longer increases and the rooting of the cuttings is basically completed.

[0041] Finally, it should be noted that the technical features of the above embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0042] The principles and implementation methods of the present invention are described in this article using specific examples. The description of the above embodiments is only used to help understand the method and core idea of ​​the present invention. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A method for rapid propagation of Acer truncatum by cuttings, characterized in that: The method comprises the steps of soaking the Acer truncatum scion in growth hormone and then cutting it into a cutting medium, and then controlling the humidity and temperature and cultivating it under natural light.

2. The breeding method according to claim 1, characterized in that: The Acer truncatum scion is a healthy young branch without diseases and insect pests that sprouts in the current year, and is obtained by retaining a bud and a leaf.

3. The breeding method according to claim 1, characterized in that: The cutting matrix is ​​perlite.

4. The breeding method according to claim 1, characterized in that: When the Acer truncatum scion is taken from a large Acer truncatum tree, the growth hormone is IBA with a concentration of 100-300 mg / L; when the Acer truncatum scion is taken from an old Acer truncatum tree, the growth hormone is ABT with a concentration of 50-500 mg / L.

5. The breeding method according to claim 4, characterized in that: The IBA concentration is 200 mg / L, and the ABT concentration is 100 mg / L.

6. The breeding method according to claim 1, characterized in that: The humidity is maintained above 85% and the temperature is 25-30°C.

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