Regulating agent and method for reducing nicotine content in tobacco leaves

Abstract

The invention provides a regulating agent and method for reducing the nicotine content in tobacco leaves, and the method for reducing the nicotine content in the tobacco leaves comprises the following steps: S1, preparing the regulating agent for reducing the nicotine content in the tobacco leaves, the regulating agent being a mixed solution of bafilomycin A1 and dicyclohexylcarbodiimide; and S2, spraying the regulating agent in the step S1 on the leaf surfaces of the whole tobacco plants in the topping period. The regulating agent can remarkably reduce the nicotine content of the tobacco leaves, solves the problem that the tobacco leaf quality is affected due to the fact that the nicotine content is too high, has small influence on agronomic characters and is beneficial to improving the market competitiveness of the tobacco leaves.

Description

Technical Field

[0001] The invention relates to the technical field of tobacco planting, and in particular to a regulating agent and method for regulating the nicotine content of flue-cured tobacco. Background Art

[0002] Flue-cured tobacco is one of the important economic crops in my country, and its quality directly affects the development of the tobacco industry and the health of consumers. Nicotine is an important alkaloid in flue-cured tobacco, and its content has an important impact on the quality and safety of flue-cured tobacco. Excessive nicotine content not only affects the taste and aroma of tobacco leaves, but also causes harm to human health. Therefore, how to effectively regulate the nicotine content of flue-cured tobacco has always been a hot issue in the field of tobacco research.

[0003] At present, in the process of flue-cured tobacco cultivation, the supply of nitrogen plays a key role in the growth, development and quality formation of flue-cured tobacco. However, in order to pursue yield, excessive nitrogen fertilizer is often applied, resulting in a decline in tobacco leaf quality, the most notable of which is the excessively high nicotine content in tobacco leaves. Some people have proposed measures such as not topping and ring cutting to reduce nicotine, but these methods have certain limitations. For example, not topping has a greater impact on tobacco leaf quality and yield, and ring cutting is time-consuming and labor-intensive and difficult to achieve in large-scale production. In recent years, some people have also explored the use of plant hormones to regulate nicotine, but the effect is unstable, the cost is high, or there is a potential impact on the environment. Therefore, there is an urgent need for a new and effective method to regulate the nicotine content of flue-cured tobacco. Summary of the invention

[0004] The object of the present invention is to provide a method for regulating the nicotine content of flue-cured tobacco. When the amount of fertilizer applied to flue-cured tobacco is high and may lead to high nicotine, a specific regulator is used to effectively reduce the nicotine content without affecting the yield and other qualities of the flue-cured tobacco.

[0005] In order to solve the above problems, the first aspect of the present invention provides a method for reducing the nicotine content in tobacco leaves, comprising the following steps:

[0006] S1. preparing a regulating agent for reducing the nicotine content in tobacco leaves, wherein the regulating agent is a mixed solution of bafilomycin A1 and dicyclohexylcarbodiimide;

[0007] S2. Use the regulating agent in S1 to spray the leaves of the tobacco plants at the topping stage.

[0008] In one embodiment, the regulator in step S1 is a mixed solution of 50 μmol / L bafilomycin A1 and 20 μmol / L dicyclohexylcarbodiimide (DCCD).

[0009] In one embodiment, in step S2, the regulating agent is evenly sprayed on the back and front of leaves of tobacco plants during the topping period of flue-cured tobacco.

[0010] The second aspect of the present invention provides a regulator for reducing the nicotine content in tobacco leaves, wherein the regulator is a mixed solution of bafilomycin A1 and dicyclohexylcarbodiimide (DCCD).

[0011] In one embodiment, the modulator is a mixed solution of 50 μmol / L bafilomycin A1 and 20 μmol / L dicyclohexylcarbodiimide (DCCD).

[0012] The beneficial effects of the present invention include:

[0013] Effectively reduce nicotine content: The regulating agent of the present invention can significantly reduce the nicotine content of tobacco leaves under conditions of high nitrogen application, thereby solving the problem that excessive nicotine content affects tobacco leaf quality.

[0014] Small impact on agronomic traits: While reducing the nicotine content, it has no obvious negative impact on agronomic traits of flue-cured tobacco such as plant height, stem girth, and leaf area, ensuring the normal growth and yield of flue-cured tobacco.

[0015] Improve economic benefits: By increasing the rate and average price of medium and high-quality tobacco, the per-acre output value of flue-cured tobacco has been significantly increased, the economic income of tobacco farmers has been increased, and it is also beneficial to improve the market competitiveness of tobacco leaves.

[0016] Environmentally friendly: The regulator itself is pollution-free, harmless, and naturally degradable, which is environmentally friendly and meets the requirements of sustainable agricultural development. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a bar graph of the nicotine content measurement results of flue-cured tobacco leaves in an embodiment of the present invention;

[0018] Figure 2a This is a photo of the tobacco plant in Comparative Example 1 14 days after topping;

[0019] Figure 2b is a photograph of the tobacco plant in Example 1 14 days after topping;

[0020] Figure 2c This is a photo of the tobacco plant in Comparative Example 2 14 days after topping;

[0021] Figure 2d is a photograph of a middle blade in an embodiment of the present invention;

[0022] Figure 2e This is a photograph of the upper blade in an embodiment of the present invention. DETAILED DESCRIPTION

[0023] The embodiments of the present application are described in detail below with reference to the accompanying drawings.

[0024] The following describes the implementation methods of the present application through specific examples, and those skilled in the art can easily understand other advantages and effects of the present application from the contents disclosed in this specification. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. The present application can also be implemented or applied through other different specific implementation methods, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present application. It should be noted that, in the absence of conflict, the following embodiments and the features in the embodiments can be combined with each other. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field without making creative work belong to the scope of protection of the present application.

[0025] It should be noted that various aspects of the embodiments within the scope of the appended claims are described below. It should be apparent that the aspects described herein can be embodied in a wide variety of forms, and any specific structure and / or function described herein is merely illustrative. Based on the present application, it should be understood by those skilled in the art that an aspect described herein can be implemented independently of any other aspect, and two or more of these aspects can be combined in various ways. For example, any number and aspect described herein can be used to implement the device and / or practice the method. In addition, other structures and / or functionalities other than one or more of the aspects described herein can be used to implement this device and / or practice this method.

[0026] It should also be noted that the illustrations provided in the following embodiments are only schematic illustrations of the basic concept of the present application. The drawings only show components related to the present application rather than being drawn according to the number, shape and size of components in actual implementation. In actual implementation, the type, quantity and proportion of each component may be changed arbitrarily, and the component layout may also be more complicated.

[0027] Additionally, in the following description, specific details are provided to facilitate a thorough understanding of the examples. However, it will be understood by those skilled in the art that the aspects described may be practiced without these specific details.

[0028] Example

[0029] This embodiment provides a method for reducing the nicotine content in tobacco leaves, comprising the following steps:

[0030] S1. preparing a regulating agent for reducing the nicotine content in tobacco leaves, wherein the regulating agent is a mixed solution of bafilomycin A1 and dicyclohexylcarbodiimide;

[0031] S2. Use the regulating agent in S1 to spray the leaves of the tobacco plants at the topping stage.

[0032] In one embodiment, the regulator in step S1 is a mixed solution of 50 μmol / L bafilomycin A1 and 20 μmol / L dicyclohexylcarbodiimide (DCCD).

[0033] In one embodiment, in step S2, the regulating agent is evenly sprayed on the back and front of leaves of tobacco plants during the topping period of flue-cured tobacco.

[0034] This embodiment also provides a regulator for reducing the nicotine content in tobacco leaves, wherein the regulator is a mixed solution of bafilomycin A1 and dicyclohexylcarbodiimide (DCCD).

[0035] In one embodiment, the modulator is a mixed solution of 50 μmol / L bafilomycin A1 and 20 μmol / L dicyclohexylcarbodiimide (DCCD).

[0036] Example 1

[0037] Using regulators to reduce nicotine content in flue-cured tobacco

[0038] The nitrogen application rate for flue-cured tobacco fields is 150 kg / hm 2 , and spray the regulator, and spray the regulator evenly on the back and front of the leaves of the tobacco plants on the day of topping. The regulator is a mixed solution of 50 μmol / L bafilomycin A1 and 20 μmol / L dicyclohexylcarbodiimide (DCCD).

[0039] Nicotine content data in Example 1 can be found in Figure 1 For economic characteristics of tobacco leaves, see Table 1, and for photos of leaves, see Figure 2d and Figure 2e .in, Figure 2d The leaf in the middle position is the middle leaf of the tobacco leaf in Example 1 14 days after topping. Figure 2e The leaves in the middle are the upper leaves of the tobacco leaves in Example 1 14 days after topping.

[0040] Comparative Example 1

[0041] Using conventional nitrogen application method to grow flue-cured tobacco

[0042] The nitrogen application rate for flue-cured tobacco fields is 105 kg / hm2 2 , do not spray regulating agents.

[0043] Nicotine content data in Comparative Example 1 refer to Figure 1 For economic characteristics of tobacco leaves, see Table 1, and for photos of leaves, see Figure 2d and Figure 2e .in, Figure 2d The leaf on the left is the middle leaf of the tobacco leaf in Comparative Example 1 14 days after topping. Figure 2eThe leaves on the left are the upper leaves of the tobacco leaves in Comparative Example 1 14 days after topping.

[0044] Comparative Example 2

[0045] Using a higher nitrogen application rate to grow flue-cured tobacco

[0046] The nitrogen application rate for flue-cured tobacco fields is 150 kg / hm2 2 , do not spray regulating agents.

[0047] Nicotine content data in Comparative Example 2 is shown in Figure 1 For economic characteristics of tobacco leaves, see Table 1, and for photos of leaves, see Figure 2d and Figure 2e .in, Figure 2d The leaf on the right is the middle leaf of the tobacco leaf in Comparative Example 2 14 days after topping. Figure 2e The leaves on the right in the figure are the upper leaves of the tobacco leaves in Comparative Example 2 14 days after topping.

[0048] Table 1 is the statistical table of economic characteristics of flue-cured tobacco leaves

[0049] Table 1

[0050]

[0051] Figure 1 It is a bar graph showing the results of measuring the nicotine content of flue-cured tobacco leaves in the embodiments of the present invention.

[0052] from Figure 1 The nicotine content of the flue-cured tobacco leaves can be seen from the nicotine content measurement results that the nicotine content (about 3%) of the high nitrogen application treatment of Comparative Example 2 is significantly higher than the nicotine content (about 2.2%) of the conventional nitrogen application treatment of Comparative Example 1; and after the treatment with the regulator, the nicotine content (about 1.4%) of the tobacco leaves treated with the high nitrogen application of Example 1 is significantly reduced, and even lower than the conventional nitrogen application treatment of Comparative Example 1. This shows that the use of the regulator solves the problem of excessive nicotine content affecting tobacco quality caused by high nitrogen.

[0053] Figure 2d 14 days after topping, the middle leaves in the examples are shown in FIG. 1 , and from left to right are the middle leaves in Comparative Example 1, Example 1, and Comparative Example 2.

[0054] Figure 2e 14 days after topping, the upper leaves in the examples are shown in Figures 14 and 14, respectively. From left to right, they are the upper leaves in Comparative Example 1, Example 1, and Comparative Example 2.

[0055] Agronomic performance: Figure 2d and Figure 2eIt can be found that the agronomic traits of the leaves in Example 1 and Comparative Example 2, such as plant height, stem girth, leaf length, leaf width, and number of leaves left, are significantly higher than those in Comparative Example 1, indicating that high nitrogen can significantly improve the agronomic traits of flue-cured tobacco.

[0056] 14 days after topping, Figure 2a Compared with the comparative example 1 shown in Figure 2c The tobacco plants treated in Comparative Example 2 shown in the figure are greedy for green and late maturing; Figure 2b The agronomic traits of tobacco plants treated with the regulating agent in Example 1 shown in the figure did not change significantly, and the yellowing time was normal, indicating that the regulating agent can regulate the nicotine content without affecting the agronomic traits, and has little effect on the agronomic traits: while reducing the nicotine content, it has no obvious negative impact on agronomic traits such as plant height, stem girth, and leaf area of ​​flue-cured tobacco, thereby ensuring the normal growth and yield of flue-cured tobacco.

[0057] Yield and quality of tobacco leaves: The quality of comparison sample 2 treated with high nitrogen application rate was significantly reduced compared with comparison sample 1 treated with conventional nitrogen application rate, which was mainly manifested in: the yield per mu increased by 8.65%, the rate of medium and high-quality tobacco decreased significantly by 59.34%, the average price decreased significantly by 13.85%, and the output value per mu decreased from 3712.91 yuan / mu to 3372.96 yuan / mu.

[0058] The quality of Example 1 after regulation and treatment was significantly improved compared with that of Comparative Example 2, wherein the rate of medium and high-quality tobacco increased significantly by 91.14%, the average price increased significantly by 14.04%, and the per-acre output value increased significantly by 17.51%; the yield decreased slightly, with the per-acre yield decreasing by 2.95%, but the difference was not significant.

[0059] In summary, although the regulators slightly inhibited the yield, they could still improve the adverse effects of excessive fertilization on flue-cured tobacco and significantly improve the quality and economic benefits of tobacco leaves.

[0060] Improve economic benefits: By increasing the rate and average price of medium and high-quality tobacco, the per-acre output value of flue-cured tobacco has been significantly increased, the economic income of tobacco farmers has been increased, and it is also beneficial to improve the market competitiveness of tobacco leaves.

[0061] The preferred embodiments of the present invention have been described in detail above, but it should be understood that after reading the above teachings of the present invention, those skilled in the art may make various changes or modifications to the present invention. These equivalent forms also fall within the scope defined by the appended claims of this application.

Claims

1. A method for reducing the nicotine content in tobacco leaves, characterized in that: The following steps are involved: S1. preparing a regulating agent for reducing the nicotine content in tobacco leaves, wherein the regulating agent is a mixed solution of bafilomycin A1 and dicyclohexylcarbodiimide; S2. Use the regulating agent in S1 to spray the leaves of the tobacco plants at the topping stage.

2. The method for reducing nicotine content in tobacco leaves according to claim 1, characterized in that: The regulator in step S1 is a mixed solution of 50 μmol / L bafilomycin A1 and 20 μmol / L dicyclohexylcarbodiimide.

3. The method for reducing nicotine content in tobacco leaves according to claim 1, characterized in that: In step S2, the regulating agent is evenly sprayed on the back and front of leaves of tobacco plants during the topping period of flue-cured tobacco.

4. A regulating agent for reducing nicotine content in tobacco leaves, characterized in that: The regulating agent is a mixed solution of bafilomycin A1 and dicyclohexylcarbodiimide.

5. The regulating agent for reducing nicotine content in tobacco leaves according to claim 4, characterized in that: The regulator is a mixed solution of 50 μmol / L bafilomycin A1 and 20 μmol / L dicyclohexylcarbodiimide.

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