Auxiliary device for leveling skull surface of small animal

Abstract

The invention relates to the technical field of medical instruments, and discloses a small animal skull surface leveling auxiliary device which comprises a shell, suspension teeth are installed at the bottom end of the shell, arc-shaped sliding grooves are formed in the suspension teeth, the bottom end of the shell is fixedly connected with fulcrums, and the fulcrums are slidably connected with the inner walls of the arc-shaped sliding grooves. Moving rods are symmetrically mounted in the bottom ends of the suspension teeth; the unique design that the suspension teeth are matched with the fulcrums is adopted, and when the bottom ends of the suspension teeth make contact with the surface of the small animal skull, the suspension teeth can automatically slide in the arc-shaped sliding grooves according to the skull radian to adjust the angle. An operator only needs to finely adjust the swing angle of the shell through the handle, and by means of visual indication of the angle pointer and the angle scales, the suspension teeth can be rapidly adjusted to be preliminarily horizontal with the surface of the skull in the vertical direction, the leveling operation process is greatly simplified, the leveling efficiency and accuracy are improved, and compared with a traditional manual repeated adjustment mode, the advantages are remarkable.

Description

Technical Field

[0001] The invention relates to the technical field of medical devices, in particular to a small animal skull surface leveling auxiliary device. Background Art

[0002] In today's field of experiments on mice and other small animals, injection into the brain of small animals is an extremely common and important operation method, and there are many different injection methods. To achieve the key goal of accurately injecting drugs into the brain of small animals, it is usually highly dependent on brain locators to determine the specific injection location. However, relying solely on brain locators is far from enough to ensure that the injection operation has sufficient accuracy and stability.

[0003] In many experimental scenarios, ensuring that the line connecting the Bregma point and the Lambda point is parallel to the forward and backward movement direction of the positioning arm of the stereotaxic positioner, and that the height of the left and right temporal bones is consistent at the same distance on both sides of the line connecting the two points, has become a key link for the success of the experiment. However, at present, this leveling process relies on repeated debugging and verification and observation of the contact between the positioning needle and the skull surface under a desktop microscope, which is a great challenge to the eyesight and time cost of the experimenters.

[0004] If the leveling cannot be done accurately, it is difficult to ensure that the drug can be accurately injected into the target location in the mouse brain according to the predetermined three-dimensional coordinates during the injection process. In particular, the deeper the injection site is and the smaller the target brain area is, the greater the deviation will be, which will seriously affect the accuracy and reliability of the experimental results, and may also cause deviations or even failure of the entire experiment, wasting a lot of experimental resources and time.

[0005] To this end, we propose a small animal skull surface leveling auxiliary device. Summary of the invention

[0006] The purpose of the present invention is to provide a small animal skull surface leveling auxiliary device to solve the problems raised in the above background technology.

[0007] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a small animal skull surface leveling auxiliary device, comprising: a shell, a suspension tooth is installed at the bottom end of the shell, an arc-shaped slide groove is opened inside the suspension tooth, a fulcrum is fixedly connected to the bottom end of the shell, and the fulcrum is slidably connected to the inner wall of the arc-shaped slide groove;

[0008] A moving rod is symmetrically installed inside the bottom end of the suspension tooth. The two moving rods are perpendicular to the suspension tooth. A positioning rod is arranged on one side of the shell. The fulcrum is slidably connected to the positioning rod.

[0009] A fixing component is provided between the suspension teeth and the two moving rods, and the fixing component is used to drive the two moving rods to approach each other to clamp the skull of the small animal, and to fix the small animal when the suspension teeth are in contact with the skull surface of the small animal;

[0010] A positioning component is installed on one side of the shell, an angle scale is opened on the top of the shell, and the positioning rod can be moved up and down to position the midpoint of the shell angle scale, the positioning rod, and the sagittal suture of the animal skull in the same plane.

[0011] Preferably, the fixed component includes a guide groove, a connecting groove, a matching groove, a guide block, a sleeve block, a first spring, a clamping groove, an L-shaped plug block and a spring sheet. The guide groove is symmetrically provided inside the suspended tooth, and the connecting groove is symmetrically provided inside the suspended tooth. The connecting groove is connected to the guide groove, and a matching groove is provided inside the connecting groove at the bottom end of the suspended tooth. A guide block is fixedly connected to the top of the movable rod, and the guide block is slidably connected to the inner wall of the guide groove. The sleeve block is slidably connected to the inner wall of the matching groove. The movable rod is slidably connected to the inside of the sleeve block, and a first spring is provided between the inner wall of the matching groove and the sleeve block. A clamping groove is provided on the inner wall of the matching groove, and an L-shaped plug block is slidably connected to the inside of the guide block. A spring is fixedly connected between the L-shaped plug block and the guide block, and the L-shaped plug block is clamped with the corresponding clamping groove.

[0012] Preferably, the positioning assembly includes a bidirectional threaded rod, a threaded slider and a support rod. One side of the shell is rotatably connected to the bidirectional threaded rod, both ends of the bidirectional threaded rod are symmetrically threadedly connected to the threaded sliders, the bottom ends of the two threaded sliders are rotatably connected to the support rod, the bottom ends of the two support rods are rotatably connected to connecting blocks, and one side of the connecting block is fixedly connected to the positioning rod.

[0013] Preferably, a transverse groove is provided inside the suspension tooth, a connecting piece is longitudinally slidably connected inside the transverse groove, and two ends of the connecting piece are respectively slidably connected to the guide block.

[0014] Preferably, the interior of the moving rod is hollow in design, a second spring is fixedly connected to the interior of the moving rod, an extension rod is slidably connected to the interior of the moving rod, and one end of the extension rod is fixedly connected to the second spring.

[0015] Preferably, one side of the suspended tooth is fixedly connected to a connecting shaft, the connecting shaft is rotatably connected to the bottom end of the shell, one end of the connecting shaft is rotatably connected to a gear, a rack is slidably connected inside the shell, the rack and the gear are meshingly connected, the top of the rack is fixedly connected to a third spring, one end of the third spring is fixedly connected to the inside of the shell, an unlocking rod is slidably connected to the inside of the suspended tooth, an avoidance groove is provided on one side of the two sets of blocks, the unlocking rod is slidably connected inside the avoidance groove, one end of the unlocking rod is fixedly connected to a pull rope, one side of the gear is fixedly connected to a turntable, the turntable is rotatably connected to the connecting shaft, one end of the pull rope is fixedly connected to one side of the turntable, and the top of the rack is fixedly connected to a push rod.

[0016] Preferably, one end of the L-shaped plug that contacts the unlocking rod is rounded, and the two L-shaped plugs are not engaged with the slots at the same time. The cross-section of the slot is designed as a right-angled trapezoid, and the inclined surface of the right-angled trapezoid faces the fulcrum.

[0017] Preferably, an angle pointer is fixedly connected to the top of the suspension tooth, and a horizontal scale is symmetrically provided on one side of the suspension tooth.

[0018] Preferably, the center of the arc-shaped slide groove coincides with the axis of the connecting shaft.

[0019] Preferably, a handle is fixedly connected to the top of the shell, a push ring is slidably connected to the outer wall of the handle, and the push ring is fixedly connected to the top of the push rod.

[0020] Preferably, the top of the handle is rotatably connected to a top column, the top of the handle is fixedly connected to a connecting column, the top of the connecting column is rotatably connected to a mounting column, the outer wall of the connecting column is provided with a pull groove, the pull groove consists of a plurality of vertical grooves and a plurality of threaded grooves, the two ends of the threaded groove are respectively connected end to end with two adjacent vertical grooves, the outer wall of the mounting column is slidably connected to a connecting sleeve, the interior of the connecting sleeve is fixedly connected to a fourth spring, one end of the fourth spring is fixedly connected to a spherical pull block, one end of the spherical pull block is slidably connected to the inner wall of the pull groove, the outer wall of the mounting column is fixedly connected to a fifth spring, one end of the fifth spring is fixedly connected to the inside of the connecting sleeve, the inner wall of the vertical groove gradually decreases in depth from top to bottom, the inner wall of the threaded groove gradually decreases in depth from top to bottom, and the bottom end of the threaded groove connected to the adjacent vertical groove is lower in depth than the bottom end of the vertical groove connected thereto, and the top end of the threaded groove connected to the adjacent vertical groove is deeper than the top end of the vertical groove connected thereto.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] 1. Accurate and efficient leveling function

[0023] Adaptive and visual leveling: The device uses the ingenious design of the suspension teeth and fulcrums, so that the suspension teeth can automatically slide and adjust the angle in the arc-shaped slide according to the curvature of the skull surface. At the same time, the combination of the angle pointer and the angle scale allows the operator to intuitively observe and accurately fine-tune, quickly achieving the initial level of the suspension teeth and the skull surface in the vertical direction, greatly improving the efficiency and accuracy of leveling, which is significantly better than the traditional manual repeated debugging method.

[0024] Coordinated precise leveling of the moving rod: Based on the preliminary leveling of the suspension teeth, the moving rod, under the coordinated action of the guide block, guide groove, connecting piece, sleeve block and the first spring, can accurately approach and contact both sides of the skull, further assisting the suspension teeth to perform fine leveling of the skull surface, ensuring that the skull surface and the suspension teeth (and shell) are precisely on the same midline, thus building a stable and reliable basic plane for subsequent experimental operations.

[0025] 2. Stable and reliable fixing performance

[0026] Strong clamping and fixation: The mobile rod is designed with a unique L-shaped plug and slot connection. Under the action of the spring, it can be firmly fixed in the appropriate clamping position, effectively preventing the head of the small animal from shaking during the experiment and providing a stable skull fixation state for experimental operations.

[0027] Overall stability is guaranteed: The moving rod and the suspension teeth work closely together, and the fixed component reliably locks the position of the moving rod, so that the entire device is tightly connected to the skull of the small animal to form a stable whole, which can effectively resist vibration and external force interference during operation, ensuring that the skull surface always maintains a horizontal state after leveling during long-term experiments, effectively ensuring the accuracy and reliability of the experimental results.

[0028] 3. Accurate 3D positioning capability

[0029] Multi-dimensional positioning basis: After leveling and skull fixation, the positioning rod is first aligned with the angle pointer to determine the vertical position. Then, the adjustment system composed of a bidirectional threaded rod, a threaded slider, a strut and a connecting block can be used to accurately move the positioning rod in the horizontal direction. The horizontal position is accurately determined from both horizontal and vertical dimensions, providing an accurate positioning reference for experimental operations based on this point.

[0030] Improve experimental accuracy: Precise positioning of the skull surface leveling helps experimenters to accurately find key positions when conducting brain-related experiments, reduce experimental errors caused by positioning deviations, and greatly improve the accuracy of experimental operations, thereby providing strong support for obtaining more accurate and scientific experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0032] Figure 2 It is a schematic diagram of the overall back structure of the present invention;

[0033] Figure 3 This is a schematic diagram of the back structure of the suspension tooth of the present invention;

[0034] Figure 4 This is one of the partial cross-sectional structural schematic diagrams of the suspension tooth of the present invention;

[0035] Figure 5 This is the second schematic diagram of the partial cross-sectional structure of the suspension tooth of the present invention;

[0036] Figure 6 It is a schematic diagram of the shell structure of the present invention;

[0037] Figure 7 This is a partial cross-sectional structural diagram of the sleeve block and the moving rod of the present invention.

[0038] Figure 8 It is a schematic diagram of the structure of a partial cross-sectional enlarged view of the suspension tooth of the present invention;

[0039] Fig. 9 for Figure 6 Enlarged view of point A in the middle;

[0040] Fig.10 for Figure 7 Enlarged view of point B in the middle;

[0041] Fig.11 It is a schematic structural diagram of a partial cross-sectional enlarged view of the connecting sleeve of the present invention.

[0042] In the figure: 1, housing; 2, suspension teeth; 3, arc-shaped slide groove; 4, fulcrum; 5, moving rod; 6, positioning rod; 7, horizontal groove; 8, connecting piece; 9, second spring; 10, extension rod; 11, connecting shaft; 12, gear; 13, rack; 14, third spring; 15, unlocking rod; 16, avoidance groove; 17, pull rope; 18, turntable; 19, angle pointer; 20, angle scale; 21, horizontal scale; 22, handle; 23, push ring; 24, ejector rod; 201, guide groove; 202, connecting groove; 203, matching groove; 204, guide block; 205, sleeve block; 206, first spring; 207, slot; 208, L-shaped plug block; 209, spring piece; 31, bidirectional threaded rod; 32, threaded slider; 33, support rod; 101, top column; 102, connecting column; 103, vertical groove; 104, threaded groove; 105, connecting sleeve; 106, fourth spring; 107, spherical dial block; 108, fifth spring. DETAILED DESCRIPTION

[0043] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] Example 1

[0045] See also Figure 1-11 As shown, a small animal skull surface leveling auxiliary device comprises a shell 1, a suspension tooth 2 is installed at the bottom end of the shell 1, an arc-shaped slide groove 3 is provided inside the suspension tooth 2, a fulcrum 4 is fixedly connected to the bottom end of the shell 1, the fulcrum 4 is slidably connected to the inner wall of the arc-shaped slide groove 3, an angle pointer 19 is fixedly connected to the top end of the suspension tooth 2, and a horizontal scale 21 is symmetrically provided on one side of the suspension tooth 2;

[0046] Moving rod 5, a moving rod 5 is symmetrically installed inside the bottom end of the suspension tooth 2, the two moving rods 5 are perpendicular to each other and the suspension tooth 2, a positioning rod 6 is arranged on one side of the housing 1, and the fulcrum 4 is slidably connected to the positioning rod 6;

[0047] The operator drives the shell 1 to swing left and right through the handle 22, so that the suspended tooth 2 in the fixed component gradually approaches the skull surface of the small animal. When the bottom end of the suspended tooth 2 contacts the skull surface, since the fulcrum 4 at the bottom end of the shell 1 is slidably connected to the inner wall of the arc-shaped slide groove 3 inside the suspended tooth 2, the suspended tooth 2 can adaptively slide around the fulcrum 4 in the arc-shaped slide groove 3. The operator fine-tunes the swing angle of the shell 1 driven by the handle 22 by observing the angle pointer 19 fixedly connected to the top of the suspended tooth 2 and the angle scale 20 opened at the top of the shell 1. For example, if the angle pointer 19 shows that the suspended tooth 2 is tilted to the left, the operator swings the shell 1 to the right through the handle 22, so that the suspended tooth 2 adjusts the angle according to the curvature of the skull surface until the angle pointer 19 scale is 0, at which time the suspended tooth 2 is initially horizontal with the skull surface in the vertical direction.

[0048] A fixing component is provided between the suspension teeth 2 and the two moving rods 5, and the fixing component is used to drive the two moving rods 5 to approach each other to clamp the skull of the small animal, and to fix the suspension teeth 2 in contact with the skull surface of the small animal;

[0049] A positioning assembly is installed on one side of the shell 1. An angle scale 20 is provided on the top of the shell 1. The positioning rod 6 can be moved up and down to position the midpoint of the angle scale 20 of the shell 1, the positioning rod 6, and the sagittal suture of the animal's skull in the same plane.

[0050] The fixing assembly includes a guide groove 201, a connecting groove 202, a matching groove 203, a guide block 204, a sleeve block 205, a first spring 206, a slot 207, an L-shaped plug block 208 and a spring 209. The guide groove 201 is symmetrically opened inside the suspension tooth 2, and the connecting groove 202 is symmetrically opened inside the suspension tooth 2. The connecting groove 202 is connected to the guide groove 201. The matching groove 203 is opened inside the connecting groove 202 at the bottom end of the suspension tooth 2. The top of the moving rod 5 is fixedly connected to the guide block 204. The guide block 204 is slidably connected to the inner wall of the guide groove 201, and the inner wall of the matching groove 203 is slidably connected with a sleeve block 205. The moving rod 5 is slidably connected to the inside of the sleeve block 205. There is a first spring 206 between the inner wall of the matching groove 203 and the sleeve block 205. The inner wall of the matching groove 203 is provided with a clamping groove 207. The guide block 204 is slidably connected with an L-shaped plug-in block 208. A spring piece 209 is fixedly connected between the L-shaped plug-in block 208 and the guide block 204. The L-shaped plug-in block 208 is clamped with the corresponding clamping groove 207.

[0051] While the suspension tooth 2 makes initial contact with the skull surface and adjusts the angle, the operator continues to apply downward force to push the device closer to the head of the small animal. The two movable rods 5 symmetrically installed inside the bottom end of the suspension tooth 2 begin to approach each other under the restriction of the guide block 204 and the guide slot 201. After the movable rods 5 contact and clamp the two sides of the skull of the small animal, they continue to move inward to a suitable clamping position. At this time, the spring piece 209 restores its deformation, and the L-shaped plug block re-enters the corresponding slot 207 under the action of the spring piece 209, thereby fixing the position of the movable rod 5. The movable rod 5 works in synergy with the suspension tooth 2 to tightly fix the skull of the small animal to prevent the head of the small animal from shaking during subsequent operations, ensuring that the entire device is tightly connected to the skull of the small animal to form a stable whole;

[0052] The positioning assembly includes a bidirectional threaded rod 31, a threaded slider 32 and a support rod 33. The bidirectional threaded rod 31 is rotatably connected to one side of the shell 1, and the two ends of the bidirectional threaded rod 31 are symmetrically threadedly connected to the threaded sliders 32. The bottom ends of the two threaded sliders 32 are rotatably connected to the support rod 33, and the bottom ends of the two support rods 33 are rotatably connected to a connecting block, and one side of the connecting block is fixedly connected to the positioning rod 6.

[0053] A transverse groove 7 is provided inside the suspension tooth 2 , and a connecting piece 8 is longitudinally slidably connected inside the transverse groove 7 , and two ends of the connecting piece 8 are slidably connected to the guide block 204 respectively.

[0054] The interior of the moving rod 5 is hollow in design, a second spring 9 is fixedly connected to the interior of the moving rod 5 , an extension rod 10 is slidably connected to the interior of the moving rod 5 , and one end of the extension rod 10 is fixedly connected to the second spring 9 .

[0055] A connecting shaft 11 is fixedly connected to one side of the suspension tooth 2, and the connecting shaft 11 is rotatably connected to the bottom end of the shell 1, and a gear 12 is rotatably connected to one end of the connecting shaft 11. A rack 13 is slidably connected inside the shell 1, and the rack 13 is meshed with the gear 12. A third spring 14 is fixedly connected to the top of the rack 13, and one end of the third spring 14 is fixedly connected to the inside of the shell 1. An unlocking rod 15 is slidably connected to the inside of the suspension tooth 2. An avoidance groove 16 is provided on one side of the two sets of blocks 205, and the unlocking rod 15 is slidably connected inside the avoidance groove 16. A pull rope 17 is fixedly connected to one end of the unlocking rod 15, and a turntable 18 is fixedly connected to one side of the gear 12. The turntable 18 is rotatably connected to the connecting shaft 11, and one end of the pull rope 17 is connected to the turntable 18. The top of the shell 1 is fixedly connected with a handle 22, and the outer wall of the handle 22 is slidably connected with a push ring 23. The push ring 23 is fixedly connected to the top of the push rod 24. When the small animal completes the experiment, it only needs to push the push ring 23 to make the push rod 24 descend, causing the rack 13 to descend together. At this time, the gear 12 meshing with it rotates, and drives the pull rope 17 through the turntable 18 to move the unlocking rod 15. When the unlocking rod 15 squeezes the rounded corner of the L-shaped plug block 208, the spring piece 209 is compressed, causing the L-shaped plug block 208 to not be plugged into the slot 207. At this time, the sleeve block 205 is reset under the action of the first spring 206.

[0056] The top of the handle 22 is rotatably connected to a top column 101, the top of the handle 22 is fixedly connected to a connecting column 102, the top of the connecting column 102 is rotatably connected to a mounting column, a pull groove is provided on the outer wall of the connecting column 102, the pull groove is composed of a plurality of vertical grooves 103 and a plurality of thread grooves 104, the two ends of the thread groove 104 are respectively connected end to end with two adjacent vertical grooves 103, a connecting sleeve 105 is slidably connected to the outer wall of the mounting column, a fourth spring 106 is fixedly connected to the interior of the connecting sleeve 105, one end of the fourth spring 106 is fixedly connected to a spherical pull block 107, one end of the spherical pull block 107 is slidably connected to the inner wall of the pull groove, a fifth spring 108 is fixedly connected to the outer wall of the mounting column, one end of the fifth spring 108 is fixedly connected to the inside of the connecting sleeve 105, the inner wall of the vertical groove 103 gradually decreases in depth from top to bottom, and the inner wall of the thread groove 104 gradually decreases in depth from top to bottom , and the depth of the bottom end of the thread groove 104 connected to the adjacent vertical groove 103 is lower than the bottom end of the vertical groove 103 connected thereto, and the depth of the top end of the thread groove 104 connected to the adjacent vertical groove 103 is higher than the top end of the vertical groove 103 connected thereto. When the bottom end of the shell 1 needs to be rotated 90 degrees, hold the connecting sleeve 105 and apply a downward force to it. At this time, the spherical block 107 inside the connecting sleeve 105 slides along the thread groove 104, causing the connecting column 102 to drive the shell 1 to rotate. Because the entire stroke of the thread groove 104 is equal to the 90-degree rotation of the connecting sleeve 105, the spherical block 107 continuously compresses the fourth spring 106. When the spherical block 107 moves to the bottom end of the vertical groove 103, no force is applied to the connecting sleeve 105 at this time. At this time, the fifth spring 108 drives the connecting sleeve 105 to reset, and the corresponding spherical block 107 moves to the top end of the vertical groove 103.

[0057] Example 2

[0058] In order to prevent the leveling device from getting stuck when resetting, this embodiment is further improved on the basis of embodiment 1. The improvements are as follows: Figure 8 and Fig.10 As shown, the end of the L-shaped plug 208 that contacts the unlocking rod 15 is provided with a rounded corner, and the two L-shaped plugs 208 are not engaged with the slot 207 at the same time. The cross-section of the slot 207 is designed as a right-angled trapezoid, and the inclined surface of the right-angled trapezoid faces the fulcrum 4. The rounded corner design of the L-shaped plug 208 is to prevent the unlocking rod 15 from squeezing the L-shaped plug 208 and causing it to get stuck. In addition, the L-shaped plugs 208 on the two sleeve blocks 205 are engaged with the slot 207 in turn, that is, one is engaged and the other is to be engaged, thereby reducing the gap between the two moving rods 5.

[0059] Working principle:

[0060] Device preparation and initial connection

[0061] Before starting the experiment, the operator first connects the connecting column 102 of the device to the horizontal arm of the brain stereotaxic instrument to ensure that the device can be stably installed and can be used for subsequent operations with the help of the brain stereotaxic instrument. At this time, the entire device is in an initial state, the suspension tooth 2 in the fixed component is located at the bottom of the shell 1, the moving rod 5 is in the initial position inside the bottom of the suspension tooth 2, and the positioning rod 6 in the positioning component is slidably connected to the fulcrum 4, and has not yet played a positioning role. The operator holds the handle 22 of the device and is ready to place it above the head of the small animal.

[0062] Leveling and fixing process

[0063] Leveling operation: The operator holds the handle 22 and moves the device above the head of a small animal such as a mouse, so that the suspension tooth 2 gradually approaches the skull surface of the small animal. When the bottom end of the suspension tooth 2 contacts the skull surface, the suspension tooth 2 can slide adaptively in the arc-shaped chute 3 around the fulcrum 4 because the fulcrum 4 at the bottom end of the shell 1 is slidably connected to the inner wall of the arc-shaped chute 3 inside the suspension tooth 2. The operator fine-tunes the position and angle of the handle 22 by observing the angle pointer 19 fixedly connected to the top of the suspension tooth 2 and the angle scale 20 opened at the top of the shell 1. For example, if the angle pointer 19 shows that the suspension tooth 2 is tilted to the left, the operator adjusts the handle 22 and swings the shell 1 to the right, so that the suspension tooth 2 is adjusted according to the curvature of the skull surface until the angle pointer 19 scale is 0, at which time the suspension tooth 2 is initially horizontal with the skull surface in the vertical direction. At the same time, the horizontal scale 21 symmetrically opened on one side of the suspension tooth 2 can assist the operator in fine-tuning the position of the device in the horizontal direction to ensure that the device fits the skull surface as closely as possible in both the horizontal and vertical directions.

[0064] Approach and fixation of the moving rod 5: While the suspension tooth 2 makes initial contact with the skull surface and adjusts the angle, the operator continues to apply downward force to push the device closer to the head of the small animal. The two moving rods 5 symmetrically installed inside the bottom of the suspension tooth 2 begin to approach each other under the restriction of the guide block 204 and the guide groove 201. The guide block 204 is fixed to the top of the moving rod 5 and slides in the guide groove 201 symmetrically opened inside the suspension tooth 2 to ensure the accurate movement direction of the moving rod 5. As the moving rod 5 approaches, the guide block 204 pushes the L-shaped plug block to compress the spring piece 209, so that the L-shaped plug block is disengaged from the slot 207. Under the action of the first spring 206, the sleeve block 205 moves relatively with the movement of the moving rod 5, and the sleeve block 205 slides with the inside of the moving rod 5 to provide buffering and adaptive capabilities for the movement of the moving rod 5. At the same time, the connecting piece 8 longitudinally slidably connected in the transverse groove 7 inside the suspension tooth 2 is slidably connected to the guide block 204 at both ends to ensure the stability of the two moving rods 5 in the process of approaching each other. The moving rod 5 continuously approaches and contacts the two sides of the skull, further assisting the suspension teeth 2 to level the skull surface, ensuring that the skull surface and the suspension teeth 2 (and then the housing 1) are located on the same midline. After the moving rod 5 contacts and clamps the two sides of the skull of the small animal, it continues to move inward to a suitable clamping position. At this time, the spring piece 209 recovers its deformation, and the L-shaped plug is re-entered into the corresponding slot 207 under the action of the spring piece 209, thereby fixing the position of the moving rod 5, achieving stable clamping of the skull of the small animal, and preventing the head of the small animal from shaking during subsequent operations.

[0065] The process of locating the midpoint between the anterior and posterior bregmas

[0066] Initial adjustment of the positioning rod 6: After completing the leveling of the suspension tooth 2 and the skull surface and fixing the skull, the positioning rod 6 is slidably connected to the fulcrum 4. The operator first adjusts the positioning rod 6 to a position that coincides with the angle pointer 19. At this time, the positioning rod 6 is in a corresponding positional relationship with the leveled skull surface in the vertical direction, preparing for the subsequent accurate determination of the position of the Bregma point (the midpoint between the anterior bregma point and the Lambda point, i.e., the posterior bregma point).

[0067] The positioning rod 6 is precisely adjusted and positioned: the bidirectional threaded rod 31 connected to the housing 1 side by rotation begins to play a role. The two ends of the bidirectional threaded rod 31 are symmetrically threaded with threaded sliders 32, and the bottom ends of the two threaded sliders 32 are connected to the support rod 33 by rotation. The connecting block connected to the bottom end of the support rod 33 is fixedly connected to the positioning rod 6. The operator rotates the bidirectional threaded rod 31. Due to the thread characteristics of the bidirectional threaded rod 31, the two threaded sliders 32 will move in opposite directions or opposite directions along the axial direction of the bidirectional threaded rod 31. The movement of the threaded slider 32 is transmitted to the connecting block through the support rod 33, thereby driving the positioning rod 6 to move in the horizontal direction. Based on experience and observation of the surface features of the skull, the operator slowly rotates the bidirectional threaded rod 31 to gradually move the positioning rod 6 until the positioning rod 6 accurately points to the Bregma point. The positioning rod 6 cooperates with the fixed components (suspended teeth 2, moving rod 5, etc.) to accurately determine the Bregma point position from the horizontal and vertical dimensions, providing a reliable positioning basis for subsequent related experimental operations based on this positioning point.

[0068] Special rotation operation

[0069] When the bottom end of the housing 1 needs to be rotated 90 degrees during the experiment, the operator performs the following operations. The operator holds the connecting sleeve 105 and applies a downward force to it. At this time, the spherical block 107 at one end of the fourth spring 106 fixedly connected inside the connecting sleeve 105 slides along the thread groove 104 in the pull groove opened on the outer wall of the connecting column 102. Since the entire stroke of the thread groove 104 is designed to be equidistant and can rotate the connecting sleeve 105 90 degrees, as the spherical block 107 slides, it continuously compresses the fourth spring 106. During the sliding process, the connecting column 102 drives the housing 1 to rotate. When the spherical block 107 moves to the bottom end where the thread groove 104 is connected to the vertical groove 103, the operator no longer applies a force to the connecting sleeve 105. At this time, one end of the fifth spring 108 fixedly connected to the outer wall of the connecting column 102 is fixedly connected to the inside of the connecting sleeve 105, and the fifth spring 108 drives the connecting sleeve 105 to reset, and the corresponding spherical block 107 moves to the top of the vertical groove 103. In this way, a 90-degree rotation of the bottom end of the shell 1 is achieved to meet special requirements for the device angle in different experimental scenarios.

[0070] After the experiment, the device is reset

[0071] When the small animal completes the experiment, the operator performs the unfixing and resetting operation of the device. The operator pushes the push ring 23 located on the outer wall of the handle 22 at the top of the housing 1. The push ring 23 is fixedly connected to the top of the push rod 24, so the push of the push ring 23 drives the push rod 24 to descend. The descent of the push rod 24 causes the rack 13 connected thereto to descend together. Since the rack 13 is meshed with the gear 12, when the rack 13 descends, the gear 12 starts to rotate. The gear 12 is fixed to one end of the connecting shaft 11, and the connecting shaft 11 is rotationally connected to one side of the suspension gear 2, and the other end of the connecting shaft 11 is fixedly connected to the turntable 18. The rotation of the gear 12 drives the connecting shaft 11 to rotate, and the rotation of the connecting shaft 11 drives the turntable 18 to rotate. When the turntable 18 rotates, the pull rope 17 wrapped around the turntable 18 is pulled. The other end of the pull rope 17 is connected to the unlocking rod 15, and the unlocking rod 15 slides inside the suspension gear 2 and is slidably connected to the avoidance groove 16 opened on one side of the sleeve block 205. The pull of the pull rope 17 moves the unlocking rod 15. When the unlocking rod 15 moves to contact the L-shaped plug-in block, since the end of the L-shaped plug-in block that contacts the unlocking rod 15 has a rounded corner, the unlocking rod 15 squeezes the rounded corner of the L-shaped plug-in block. At this time, the L-shaped plug-in block is subjected to a squeezing force, which compresses the spring sheet 209, causing the L-shaped plug-in block to disengage from the slot 207 and no longer plug into the slot 207. At this time, the sleeve block 205 slides and resets in a direction away from the moving rod 5 under the action of the first spring 206, and the moving rod 5 is no longer fixed. The device returns to the initial state, which is convenient for subsequent cleaning, storage and use in the next experiment.

[0072] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0073] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A small animal skull surface leveling auxiliary device, characterized in that: include: A shell (1), a suspension tooth (2) is installed at the bottom end of the shell (1), an arc-shaped slide groove (3) is provided inside the suspension tooth (2), a fulcrum (4) is fixedly connected to the bottom end of the shell (1), and the fulcrum (4) is slidably connected to the inner wall of the arc-shaped slide groove (3); A moving rod (5) is symmetrically installed inside the bottom end of the suspension tooth (2), the two moving rods (5) and the suspension tooth (2) are perpendicular to each other, a positioning rod (6) is arranged on one side of the housing (1), and the fulcrum (4) and the positioning rod (6) are slidably connected; A fixing component is provided between the suspension tooth (2) and the two moving rods (5), and the fixing component is used to drive the two moving rods (5) to move closer to each other to clamp the skull of the small animal, and then cooperate with the suspension tooth (2) to contact the surface of the skull of the small animal to form a fixation; A positioning component is installed on one side of the shell (1), an angle scale (20) is provided on the top of the shell (1), and a positioning rod (6) can be moved up and down to position the midpoint of the angle scale (20) of the shell (1), the positioning rod (6), and the sagittal suture of the animal's skull in the same plane.

2. The small animal skull surface leveling auxiliary device according to claim 1, characterized in that: The fixing assembly comprises a guide groove (201), a connecting groove (202), a matching groove (203), a guide block (204), a sleeve block (205), a first spring (206), a clamping groove (207), an L-shaped plug block (208) and a spring piece (209); the guide groove (201) is symmetrically provided inside the suspension tooth (2); the connecting groove (202) is symmetrically provided inside the suspension tooth (2); the connecting groove (202) is communicated with the guide groove (201); a matching groove (203) is provided inside the connecting groove (202) at the bottom end of the suspension tooth (2); the top end of the moving rod (5) is fixedly connected to the guide block (201); 4), the guide block (204) is slidably connected to the inner wall of the guide groove (201), the inner wall of the matching groove (203) is slidably connected to a sleeve block (205), the moving rod (5) is slidably connected to the inside of the sleeve block (205), a first spring (206) is provided between the inner wall of the matching groove (203) and the sleeve block (205), a clamping groove (207) is provided on the inner wall of the matching groove (203), an L-shaped plug-in block (208) is slidably connected to the inside of the guide block (204), a spring piece (209) is fixedly connected between the L-shaped plug-in block (208) and the guide block (204), and the L-shaped plug-in block (208) is clamped with the corresponding clamping groove (207).

3. The small animal skull surface leveling auxiliary device according to claim 1, characterized in that: The positioning assembly comprises a bidirectional threaded rod (31), a threaded slider (32) and a support rod (33); one side of the housing (1) is rotatably connected to the bidirectional threaded rod (31); both ends of the bidirectional threaded rod (31) are symmetrically threadedly connected to the threaded sliders (32); the bottom ends of the two threaded sliders (32) are both rotatably connected to the support rod (33); the bottom ends of the two support rods (33) are rotatably connected to a connecting block; one side of the connecting block is fixedly connected to the positioning rod (6).

4. The small animal skull surface leveling auxiliary device according to claim 2, characterized in that: A transverse groove (7) is provided inside the suspension tooth (2), a connecting piece (8) is longitudinally slidably connected inside the transverse groove (7), and two ends of the connecting piece (8) are respectively slidably connected to the guide block (204).

5. The small animal skull surface leveling auxiliary device according to claim 1, characterized in that: The interior of the moving rod (5) is hollow in design, a second spring (9) is fixedly connected to the interior of the moving rod (5), an extension rod (10) is slidably connected to the interior of the moving rod (5), and one end of the extension rod (10) is fixedly connected to the second spring (9).

6. The small animal skull surface leveling auxiliary device according to claim 2, characterized in that: A connecting shaft (11) is fixedly connected to one side of the suspension tooth (2), the connecting shaft (11) is rotatably connected to the bottom end of the housing (1), one end of the connecting shaft (11) is rotatably connected to a gear (12), a rack (13) is slidably connected inside the housing (1), the rack (13) and the gear (12) are meshedly connected, a third spring (14) is fixedly connected to the top of the rack (13), one end of the third spring (14) is fixedly connected to the inside of the housing (1), an unlocking rod (15) is slidably connected inside the suspension tooth (2), and the two gears are meshedly connected to each other. A relief groove (16) is provided on one side of the sleeve block (205), the unlocking rod (15) is slidably connected inside the relief groove (16), one end of the unlocking rod (15) is fixedly connected to a pull rope (17), one side of the gear (12) is fixedly connected to a turntable (18), the turntable (18) is rotatably connected to the connecting shaft (11), one end of the pull rope (17) is fixedly connected to one side of the turntable (18), the top end of the rack (13) is fixedly connected to a push rod (24), and the center of the arc-shaped slide groove (3) coincides with the axis of the connecting shaft (11).

7. The small animal skull surface leveling auxiliary device according to claim 2, characterized in that: One end of the L-shaped plug (208) that contacts the unlocking rod (15) is provided with a rounded corner, and the two L-shaped plugs (208) are not engaged with the slot (207) at the same time. The slot (207) has a right-angled trapezoidal cross-section, and the inclined surface of the right-angled trapezoid faces the fulcrum (4).

8. The small animal skull surface leveling auxiliary device according to claim 1, characterized in that: An angle pointer (19) is fixedly connected to the top of the suspension tooth (2), and a horizontal scale (21) is symmetrically provided on one side of the suspension tooth (2).

9. The small animal skull surface leveling auxiliary device according to claim 6, characterized in that: The top end of the housing (1) is fixedly connected with a handle (22), the outer wall of the handle (22) is slidably connected with a push ring (23), and the push ring (23) is fixedly connected with the top end of a push rod (24).

10. The small animal skull surface leveling auxiliary device according to claim 9, characterized in that: The top of the handle (22) is rotatably connected to a top column (101), the top of the handle (22) is fixedly connected to a connecting column (102), the top of the connecting column (102) is rotatably connected to a mounting column, the outer wall of the connecting column (102) is provided with a shifting groove, the shifting groove is composed of a plurality of vertical grooves (103) and a plurality of threaded grooves (104), the two ends of the threaded groove (104) are respectively connected end to end with two adjacent vertical grooves (103), the outer wall of the mounting column is slidably connected to a connecting sleeve (105), the interior of the connecting sleeve (105) is fixedly connected to a fourth spring (106), one end of the fourth spring (106) is fixedly connected to a spherical A shift block (107), one end of the spherical shift block (107) is slidably connected to the inner wall of the shift groove, the outer wall of the mounting column is fixedly connected with a fifth spring (108), one end of the fifth spring (108) is fixedly connected to the inside of the connecting sleeve (105), the inner wall of the vertical groove (103) gradually decreases in depth from top to bottom, the inner wall of the thread groove (104) gradually decreases in depth from top to bottom, and the bottom end of the thread groove (104) connected to the adjacent vertical groove (103) has a lower depth than the bottom end of the vertical groove (103) connected thereto, and the top end of the thread groove (104) connected to the adjacent vertical groove (103) has a higher depth than the top end of the vertical groove (103) connected thereto.

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