Cedrol hair growth liquid composition as well as preparation method and application thereof

Abstract

The invention discloses a cedrol hair-growing liquid composition and a preparation method and application thereof, and belongs to the technical field of cosmetic preparation.According to the cedrol hair-growing liquid composition, a formula of a cedrol and cacumen biotae extract composition with the hair-growing effect is deeply optimized by adopting two-time extreme vertex design, and the cedrol and cacumen biotae extract composition with the hair-growing effect is obtained by taking viscosity as a core evaluation index. Uniform and stable yellow transparent cedrol and cacumen biotae extract hair growth liquid is obtained; the cedrol and cacumen biotae extract hair growth liquid is moderate in viscosity (more than 200cP), the drug loading capacity of cedrol is 0.5%-10%, the net content of the cacumen biotae extract is 0.1%-2%, and a foundation is laid for external application of the cedrol and cacumen biotae extract composition to head skin and hair growth promotion.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetic preparation, and particularly relates to a cedar alcohol hair growth liquid composition and a preparation method and application thereof. Background Art

[0002] Cedrol (CE), also known as cedarwood, cedar alcohol, CAS number: 77-53-2, is a high-boiling sesquiterpene alcohol with a weak aroma and a mild fir aroma. It is mostly found in volatile oils of plants such as Cupressaceae, Taxaceae and Pinaceae. Cedrol has been approved by the US Food and Drug Administration as a flavoring agent or adjuvant, and has been widely used as a flavoring ingredient in cosmetics, food and medicines. It is also widely used as a flavoring agent for disinfectants and sanitary products. It is reported that cedrol has a wide range of pharmacological activities, including promoting hair growth, anti-inflammatory and analgesic, treating cardiovascular and cerebrovascular diseases, and anti-tumor effects. However, cedrol is almost insoluble in water, and its poor solubility in water limits its application. Therefore, screening a suitable solvent system and increasing its solubility is an effective way to increase its application range.

[0003] Platycladus orientalis leaf, as a traditional Chinese medicine with a long history, is taken from the leaves of the Platycladus orientalis tree. Classics such as "Compendium of Materia Medica" and "Compendium of Materia Medica" have recorded its effects on hair growth and black hair. Modern scientific research has further confirmed that Platycladus orientalis leaf extract can effectively promote hair growth by promoting hair follicle proliferation, improving the scalp environment, and having antioxidant and antibacterial effects. Platycladus orientalis leaf extract has been widely used in the field of cosmetics and personal care products, mainly as a hair conditioner and skin protectant. This raw material has been included in the "Catalogue of Used Cosmetic Raw Materials (2021 Edition)". At the same time, it does not belong to the banned substances in the "Technical Specifications for Safety of Cosmetics (2015 Edition)". Summary of the invention

[0004] In order to solve the above problems, the present invention provides a cedar alcohol hair growth liquid composition and an optimized preparation method and application thereof.

[0005] The purpose of the present invention is achieved by the following technical solutions:

[0006] A cedar alcohol hair growth liquid composition, which comprises, by weight ratio: 2-methyl-1,3-propanediol: 1,3-butanediol: 2-methyl-2,4-pentanediol: glycerol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = (18-25): (25-31): (7-10): (20-29): (0.2-0.8): (0.2-0.8): (4-15): (0.8-15), wherein the net content of Platycladus orientalis leaf extract in the cedar alcohol hair growth liquid composition is 0.1-2%.

[0007] Furthermore, the viscosity of the cedar alcohol hair growth liquid composition is above 200 cP, the cedar alcohol drug loading is 0.5% to 10%, and the net content of Platycladus orientalis leaf extract in the cedar alcohol hair growth liquid composition is 2%.

[0008] Furthermore, a cedar alcohol hair growth liquid composition comprises, by weight ratio: 2-methyl-1,3-propanediol: 1,3-butanediol: 2-methyl-2,4-pentanediol: glycerol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = (18-22): (26-29): (8-9): (23-25): 0.50: 0.50: 10.00: (8-9).

[0009] Furthermore, a cedar alcohol hair growth liquid composition is provided, wherein the composition is calculated by weight ratio as follows: 2-methyl-1,3-propanediol: 1,3-butanediol: 2-methyl-2,4-pentanediol: glycerol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = 22.00: 26.50: 9.00: 23.50: 0.50: 0.50: 10.00: 8.00, wherein the net content of Platycladus orientalis leaf extract in the cedar alcohol hair growth liquid composition is 2%.

[0010] Furthermore, a cedar alcohol hair growth liquid composition is provided, wherein the composition is calculated by weight ratio as follows: 2-methyl-1,3-propanediol: 1,3-butanediol: 2-methyl-2,4-pentanediol: glycerol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = 18.00: 29.00: 8.81: 25.00: 0.50: 0.50: 10.00: 8.19, wherein the net content of Platycladus orientalis leaf extract in the cedar alcohol hair growth liquid composition is 2%.

[0011] The preparation method of the cedar alcohol hair growth liquid composition of the present invention comprises the following steps:

[0012] a. Accurately weigh 2-methyl-1,3-propanediol, 1,3-butanediol, 2-methyl-2,4-pentanediol, 1,2-hexanediol, and p-hydroxyacetophenone and mix them; stir at room temperature, assisted by ultrasound, or stir at 45°C until p-hydroxyacetophenone is completely dissolved to obtain sample 1;

[0013] b. Accurately weigh cedar alcohol and add it to sample 1; stir at room temperature, assisted by ultrasound, or stir at 45°C until the cedar alcohol is completely dissolved to obtain sample 2;

[0014] c. Accurately weigh glycerol and add it to sample 2; stir at room temperature, with the aid of ultrasound, or stir at 45°C until the sample is evenly mixed to obtain sample 3;

[0015] d. Accurately weigh the Platycladus orientalis leaf extract solution and add it to sample 3, stir at room temperature until the sample is evenly mixed to obtain a cedar alcohol hair growth liquid composition with a viscosity of more than 200 cP.

[0016] Furthermore, the ultrasound in step a has a frequency of 40 kHz, a power of 200 W, and an ultrasound time of 5 min.

[0017] Furthermore, the ultrasound in step b has a frequency of 40 kHz, a power of 200 W, and an ultrasound time of 5 min.

[0018] Furthermore, in the step c, the ultrasound frequency is 40kHz, the power is 200w, and the ultrasound time is 10min; the net content of the added Platycladus orientalis leaf extract in the cedar alcohol hair growth liquid composition is 0.1% to 2%.

[0019] The present invention also provides an application of the cedar alcohol hair growth liquid composition in the preparation of cosmetics for promoting the growth of head hair.

[0020] The cedar alcohol hair growth liquid composition of the present invention belongs to a non-oily (alcohol base) matrix and is suitable for oily skin.

[0021] The beneficial effects of the present invention are:

[0022] The present invention prepares a hair growth liquid by mixing different solvents in a certain proportion, and uses viscosity and drug loading as evaluation indicators. The formula of the hair growth liquid is optimized by using extreme vertex mixing design to ensure that cedar alcohol can be stably dispersed in the mixed solvent system. The drug loading is successfully increased to 10%, and 2% (net content) of Platycladus orientalis leaf extract is added. The cedar alcohol hair growth liquid composition thus prepared can be used to prepare cosmetics that promote head hair growth.

[0023] Among them, the present invention firstly screened the solvents through the cedar alcohol solubility experiment. On this basis, two rounds of extreme vertex mixing design were adopted to comprehensively investigate the effects of different solvents on the viscosity of the hair growth solution and the cedar alcohol loading, thereby screening out the optimal solvent ratio. Then, the addition amount of Platycladus orientalis leaf extract was screened and its optimal addition amount was determined. The present invention uses a mixed solvent method to prepare a hair growth solution containing cedar alcohol and Platycladus orientalis leaf extract. The viscosity of the hair growth solution finally prepared reached more than 200cP, the cedar alcohol loading amount was 10%, and the Platycladus orientalis leaf extract addition amount was 2% (net content). A research report pointed out that the solubility of cedar alcohol is 21.88mg / kg (CN112870161A). The present invention achieves a cedar alcohol loading amount of up to 100g / kg by dissolving 1000mg of cedar alcohol in 9.0g of mixed solvent, which significantly improves the solubility of cedar alcohol. The prepared hair growth solution is mainly used for external use on the scalp to promote hair growth. Since cedar alcohol has poor solubility in water, using a mixed solvent to dissolve it can effectively enhance its dissolution effect. In addition, ingredients such as 2-methyl-1,3-propanediol, glycerol, and 1,2-hexanediol in the mixed solvent also have the effect of moisturizing and protecting the skin.

[0024] Finally, the cedar alcohol hair growth liquid composition disclosed by the present invention does not contain any preservatives. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 is the structural formula of cedrol used in the present invention.

[0026] Figure 2 Hair growth experiment roadmap (cyclophosphamide model).

[0027] Figure 3 Effects of different samples on hair length and weight of mice. a. Images of the back of mice at 0, 9, 12, 16 and 28 days after back hair removal; b. Evaluation of the effects of different samples on promoting hair growth in mice (the vertical axis is the grayscale value read by Image J software, without units); c. Effect of cedrol on hair length of mice with alopecia areata; *Compared with the control group, ***p<0.001; #Compared with the control group###p<0.001, ##p<0.01, ###p<0.001. DETAILED DESCRIPTION

[0028] The specific embodiments of the present invention are further described in detail below, but the present invention is not limited to these embodiments, and any improvement or substitution based on the basic spirit of the present embodiment still falls within the scope of protection claimed by the present invention.

[0029] 1. Screening formula of cedar alcohol hair growth liquid composition:

[0030] 1. The first extreme vertex design: 2-methyl-1,3-propanediol, polyethylene glycol-400 and 1,3-butanediol were selected as the influencing factors X1, X2 and X3 respectively, and the viscosity parameter was the dependent variable Y1. Among them, X1 = 0-45%, X2 = 0-60%, and X3 = 0-87.8% were fixed. The vertex mixture design extreme vertex mixture design method in Minitab 21.1 was used to measure the sample viscosity and optimize the formula of the cedar alcohol hair growth liquid composition. The results are shown in Table 1:

[0031] Table 1 The first extreme vertex design

[0032]

[0033]

[0034] 2. Second extreme vertex mixture design: Select 2-methyl-1,3-propanediol and 1,3-butanediol mixed solution (0.45:0.55), 2-methyl-2,4-pentanediol, and glycerol as influencing factors X1, X2, and X3, respectively, and take viscosity parameters and whether the drug loading can reach 10% as dependent variables Y1 and Y2. Among them, X1 = 17.9-100%, X2 = 0-20%, and X3 = 0-62.1%. The vertex mixture design extreme vertex mixture design method in Minitab 21.1 was used to optimize the formula of the cedar alcohol hair growth liquid composition and measure the viscosity. The results are shown in Table 2:

[0035] Table 2 Second extreme vertex design

[0036]

[0037] As shown in Table 2, the drug loading of Prescription 2, Prescription 4-6 and Prescription 9 can reach 10%. Among them, Prescription 5 has a viscosity of more than 200 cP and a drug loading of 10%.

[0038] The drug loading in Table 2 refers to the amount of cedarol dissolved in the mixed solvent, which is calculated as cedarol mass / (total solvent + cedarol mass).

[0039] 3. 0.5% of 1,2-hexanediol and 0.5% of p-hydroxyacetophenone were added as antioxidants.

[0040] 4. Add 8% of Platycladus orientalis leaf extract solution, wherein the net content of Platycladus orientalis leaf extract is 2%, and the solvent for dissolving the Platycladus orientalis leaf extract is 1,2-propylene glycol; the Platycladus orientalis leaf extract solution is purchased from Hunan Langlin Biological Resources Co., Ltd.

[0041] Table 3 Screening table of the amount of Platycladus orientalis leaf extract added

[0042]

[0043] 2. Solubility and viscosity experiments of solvent cedarol and Platycladus orientalis leaf extract

[0044] 1. The first solvent solubility experiment: After experimental verification, at room temperature, the solubility of cedar alcohol in 2-methyl-1,3-propanediol, polyethylene glycol-400 and 1,3-butanediol is about 25 mg / g.

[0045] 2. Solvent viscosity experiment: Measure the viscosity of 2-methyl-1,3-propanediol, polyethylene glycol-400, and 1,3-butanediol, and repeat three times. Viscosity measurement method using a rotational viscometer: Measure 7mL of sample and add it to the measuring cup, select rotor 18, the water bath temperature is 25°C, and the measuring speed is 10rpm. The results are shown in Table 4 below:

[0046] Table 4 Viscosity of 2-methyl-1,3-propanediol, polyethylene glycol-400, and 1,3-butanediol

[0047]

[0048] 3. Second solvent solubility exploration experiment: After experimental verification, at room temperature, the solubility of cedar alcohol in 1,2-propylene glycol, glycerol, diethylene glycol monoethyl ether and 2-methyl-2,4-pentanediol is 0.05g / g, <0.005g / g, 0.5g / g and 0.25g / g respectively. The results are shown in Table 5 below:

[0049] Table 5 Solubility of propylene glycol, glycerol, diethylene glycol monoethyl ether and 2-methyl-2,4-pentanediol

[0050] Sample name Ultrasonic solubility g / g Stirring solubility g / g 1,2-Propanediol 0.05 0.05 Glycerin ＜＜0.005 — Diethylene glycol monoethyl ether 0.74 0.5 2-Methyl-2,4-pentanediol 0.50 0.25

[0051] 4. Solvent viscosity experiment: The viscosity of propylene glycol, glycerol, diethylene glycol monoethyl ether and 2-methyl-2,4-pentanediol was measured three times. The results are shown in Table 6 below:

[0052] Table 6 Viscosity of propylene glycol, glycerol, diethylene glycol monoethyl ether and 2-methyl-2,4-pentanediol

[0053]

[0054] 5. From the experimental results, it can be seen that 2-methyl-1,3-propanediol and 1,3-butanediol have good viscosity and solubility, so 2-methyl-1,3-propanediol and 1,3-butanediol are used as the best solvents for cedar alcohol. 2-methyl-2,4-pentanediol can improve the solubility of cedar alcohol in a mixed solvent of 2-methyl-1,3-propanediol and 1,3-butanediol, and its viscosity is higher than that of diethylene glycol monoethyl ether, so it is used as the best solubilizer, and glycerol (glycerol) is used as a thickener. The above selection can take into account both fluidity and adhesion. At the same time, according to Table 3, the appropriate addition amount of Platycladus orientalis leaf extract is 2%.

[0055] Example 1

[0056] A cedar alcohol hair growth liquid composition, comprising, by weight ratio: 2-methyl-1,3-propanediol: 1,3-butanediol: 2-methyl-2,4-pentanediol: glycerol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = 22.00: 26.50: 9.00: 23.50: 0.50: 0.50: 10.00: 8.00, wherein the net content of Platycladus orientalis leaf extract is 2%;

[0057] Specifically: 2.20g of 2-methyl-1,3-propanediol, 2.65g of 1,3-butanediol, 0.90g of 2-methyl-2,4-pentanediol, 2.35g of glycerol, 0.05g of 1,2-hexanediol, 0.05g of p-hydroxyacetophenone, 1.00g of cedar alcohol, and 0.80g of Platycladus orientalis leaf extract solution. The net content of Platycladus orientalis leaf extract is 2%.

[0058] The preparation method of the cedar alcohol hair growth liquid composition comprises the following steps:

[0059] a. Accurately weigh 2-methyl-1,3-propanediol, 1,3-butanediol, 2-methyl-2,4-pentanediol, 1,2-hexanediol, and p-hydroxyacetophenone and mix them; stir at room temperature, assisted by ultrasound, with an ultrasound frequency of 40 kHz, an ultrasound power of 200 w, and an ultrasound time of 5 min; until the p-hydroxyacetophenone is completely dissolved to obtain sample 1;

[0060] b. Accurately weigh cedar alcohol and add it to sample 1; stir at room temperature, assisted by ultrasound, the ultrasonic frequency is 40kHz, the ultrasonic power is 200w, and the ultrasonic time is 10min; until the cedar alcohol is completely dissolved to obtain sample 2;

[0061] c. Accurately weigh glycerol and add it to sample 2; stir at room temperature, assisted by ultrasound, the ultrasonic frequency is 40kHz, the ultrasonic power is 200w, and the ultrasonic time is 10min; until the sample is evenly mixed to obtain sample 3;

[0062] d. Accurately weigh the Platycladus orientalis leaf extract solution and add it to sample 3 to make the mass fraction of Platycladus orientalis leaf extract be 2%, stir at room temperature until the sample is evenly mixed to obtain a cedar alcohol hair growth liquid composition.

[0063] The viscosity of the cedar alcohol hair growth liquid composition prepared in Example 1 is 243.00 cP, the cedar alcohol drug loading is 10%, and the net content of Platycladus orientalis leaf extract is 2%.

[0064] Example 2

[0065] A cedar alcohol hair growth liquid composition, comprising, by weight ratio: 2-methyl-1,3-propanediol: 1,3-butanediol: 2-methyl-2,4-pentanediol: glycerol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = 24.74: 30.23: 9.32: 28.95: 0.50: 0.50: 4.96: 0.80, wherein the net content of Platycladus orientalis leaf extract is 0.2%;

[0066] Specifically: 2.47g 2-methyl-1,3-propanediol, 3.02g 1,3-butanediol, 0.93g 2-methyl-2,4-pentanediol, 2.90g glycerol, 0.05g 1,2-hexanediol, 0.05g p-hydroxyacetophenone, 0.50g cedarol, 0.08g Platycladus orientalis leaf extract solution, the net content of Platycladus orientalis leaf extract is 0.2%.

[0067] The preparation method of the cedar alcohol hair growth liquid composition comprises the following steps:

[0068] a. Accurately weigh 2-methyl-1,3-propanediol, 1,3-butanediol, 2-methyl-2,4-pentanediol, 1,2-hexanediol, and p-hydroxyacetophenone and mix them; stir at room temperature, assisted by ultrasound, with an ultrasound frequency of 40 kHz, an ultrasound power of 200 w, and an ultrasound time of 5 min; until the p-hydroxyacetophenone is completely dissolved to obtain sample 1;

[0069] b. Accurately weigh cedar alcohol and add it to sample 1; stir at room temperature, assisted by ultrasound, the ultrasonic frequency is 40kHz, the ultrasonic power is 200w, and the ultrasonic time is 10min; until the cedar alcohol is completely dissolved to obtain sample 2;

[0070] c. Accurately weigh glycerol and add it to sample 2; stir at 45°C until the sample is evenly mixed to obtain sample 3;

[0071] d. Accurately weigh the Platycladus orientalis leaf extract solution and add it to sample 3 to make the mass fraction of Platycladus orientalis leaf extract be 0.2%, stir at room temperature until the sample is evenly mixed to obtain a cedar alcohol hair growth liquid composition.

[0072] The viscosity of the cedar alcohol and Platycladus orientalis leaf extract hair growth liquid prepared in Example 2 is 213.00 cP, the drug loading of cedar alcohol is 5%, and the net content of Platycladus orientalis leaf extract is 0.2%

[0073] Example 3

[0074] A cedar alcohol hair growth liquid composition comprises, by weight ratio: 2-methyl-1,3-propanediol: 1,3-butanediol: 2-methyl-2,4-pentanediol: glycerol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = 18.00: 29.00: 8.81: 25.00: 0.50: 0.50: 10.00: 8.19, wherein the net content of Platycladus orientalis leaf extract is 2%;

[0075] Specifically: 1.80g of 2-methyl-1,3-propanediol, 2.90g of 1,3-butanediol, 0.88g of 2-methyl-2,4-pentanediol, 2.50g of glycerol, 0.05g of 1,2-hexanediol, 0.05g of p-hydroxyacetophenone, 1.00g of cedar alcohol, and 0.82g of Platycladus orientalis leaf extract solution. The net content of Platycladus orientalis leaf extract is 2%.

[0076] The preparation method of the cedar alcohol hair growth liquid composition comprises the following steps:

[0077] a. Accurately weigh 2-methyl-1,3-propanediol, 1,3-butanediol, 2-methyl-2,4-pentanediol, 1,2-hexanediol, and p-hydroxyacetophenone and mix them; stir at room temperature, assisted by ultrasound, with an ultrasound frequency of 40 kHz, an ultrasound power of 200 w, and an ultrasound time of 5 min; until the p-hydroxyacetophenone is completely dissolved to obtain sample 1;

[0078] b. Accurately weigh cedar alcohol and add it to sample 1; stir at room temperature, assisted by ultrasound, the ultrasonic frequency is 40kHz, the ultrasonic power is 200w, and the ultrasonic time is 10min; until the cedar alcohol is completely dissolved to obtain sample 2;

[0079] c. Accurately weigh glycerol and add it to sample 2; stir at room temperature, assisted by ultrasound, the ultrasonic frequency is 40kHz, the ultrasonic power is 200w, and the ultrasonic time is 10min; until the sample is evenly mixed to obtain sample 3;

[0080] d. Accurately weigh the Platycladus orientalis leaf extract solution and add it to sample 3 to make the mass fraction of Platycladus orientalis leaf extract be 2%, stir at room temperature until the sample is evenly mixed to obtain a cedar alcohol hair growth liquid composition.

[0081] The viscosity of the cedar alcohol hair growth liquid composition prepared in Example 3 is 220.42 cP, the cedar alcohol drug loading is 10%, and the net content of Platycladus orientalis leaf extract is 2%.

[0082] Comparative Example 1

[0083] A cedar alcohol hair growth liquid composition comprises, by weight: 2-methyl-1,3-propanediol: 1,3-butanediol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = 41.96: 51.28: 0.50: 0.50: 4.96: 0.80, and the net content of Platycladus orientalis leaf extract is 0.2%; the viscosity of the obtained cedar alcohol hair growth liquid composition is 116.46 cP, the cedar alcohol drug loading is 5%, and the net content of Platycladus orientalis leaf extract is 0.2%;

[0084] Specifically: 4.20 g of 2-methyl-1,3-propanediol, 5.13 g of 1,3-butanediol, 0.05 g of 1,2-hexanediol, 0.05 g of p-hydroxyacetophenone, 0.50 g of cedar alcohol, and 0.08 g of Platycladus orientalis leaf extract solution.

[0085] The preparation method of the cedar alcohol hair growth liquid composition comprises the following steps:

[0086] a. Accurately weigh 2-methyl-1,3-propanediol, 1,3-butanediol, 2-methyl-2,4-pentanediol, 1,2-hexanediol, and p-hydroxyacetophenone and mix them; stir at 45°C until p-hydroxyacetophenone is completely dissolved to obtain sample 1;

[0087] b. Accurately weigh cedar alcohol and add it to sample 1; stir at 45°C until the cedar alcohol is completely dissolved to obtain sample 2;

[0088] c. Accurately weigh the Platycladus orientalis leaf extract solution and add it to sample 2 to make the mass fraction of Platycladus orientalis leaf extract be 0.2%, stir at room temperature until the sample is evenly mixed to obtain a cedar alcohol hair growth liquid composition.

[0089] Comparative Example 2

[0090] A cedar alcohol hair growth liquid composition, comprising, by weight: 2-methyl-1,3-propanediol: 1,3-butanediol: 2-methyl-2,4-pentanediol: glycerol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = 32.94: 40.26: 4.61: 14.31: 0.49: 0.49: 4.90: 2.01, the net content of Platycladus orientalis leaf extract is 0.5%; the viscosity of the obtained cedar alcohol hair growth liquid composition is 150.01 cP, the cedar alcohol drug loading is 5%, and the net content of Platycladus orientalis leaf extract is 0.5%;

[0091] Specifically: 3.29g of 2-methyl-1,3-propanediol, 4.03g of 1,3-butanediol, 0.46g of 2-methyl-2,4-pentanediol, 1.43g of glycerol, 0.05g of 1,2-hexanediol, 0.05g of p-hydroxyacetophenone, 0.49g of cedar alcohol, and 0.20g of Platycladus orientalis leaf extract solution.

[0092] The preparation method of the cedar alcohol hair growth liquid composition comprises the following steps:

[0093] a. Accurately weigh 2-methyl-1,3-propanediol, 1,3-butanediol, 2-methyl-2,4-pentanediol, 1,2-hexanediol, and p-hydroxyacetophenone and mix them; stir at room temperature, assisted by ultrasound, with an ultrasound frequency of 40 kHz, an ultrasound power of 200 w, and an ultrasound time of 5 min; until the p-hydroxyacetophenone is completely dissolved to obtain sample 1;

[0094] b. Accurately weigh cedar alcohol and add it to solution a; stir at room temperature, assisted by ultrasound, the ultrasonic frequency is 40kHz, the ultrasonic power is 200w, and the ultrasonic time is 10min; until the cedar alcohol is completely dissolved to obtain sample 2;

[0095] c. Accurately weigh glycerol and add it to sample 2, stir at room temperature, and perform ultrasound with an ultrasound frequency of 40 kHz, an ultrasound power of 200 w, and an ultrasound time of 10 min until the sample is evenly mixed to obtain sample 3;

[0096] d. Accurately weigh the Platycladus orientalis leaf extract solution and add it to the sample 3 to make the mass fraction of Platycladus orientalis leaf extract be 0.5%, stir at room temperature until the sample is evenly mixed to obtain a cedar alcohol hair growth liquid composition.

[0097] Comparative Example 3

[0098] A cedar alcohol hair growth liquid composition, comprising, by weight: 2-methyl-1,3-propanediol: 1,3-butanediol: 2-methyl-2,4-pentanediol: glycerol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = 29.27: 35.77: 14.04: 14.54: 0.50: 0.50: 4.98: 0.40, with a net content of Platycladus orientalis leaf extract of 0.1%; the viscosity of the obtained cedar alcohol hair growth liquid composition is 144.53 cP, the cedar alcohol drug loading is 5%, and the net content of Platycladus orientalis leaf extract is 0.1%;

[0099] Specifically: 2.93g of 2-methyl-1,3-propanediol, 3.58g of 1,3-butanediol, 1.40g of 2-methyl-2,4-pentanediol, 1.45g of glycerol, 0.05g of 1,2-hexanediol, 0.05g of p-hydroxyacetophenone, 0.50g of cedar alcohol, and 0.04g of Platycladus orientalis leaf extract solution.

[0100] The preparation method of the cedar alcohol hair growth liquid composition comprises the following steps:

[0101] a. Accurately weigh 2-methyl-1,3-propanediol, 1,3-butanediol, 2-methyl-2,4-pentanediol, 1,2-hexanediol, and p-hydroxyacetophenone and mix them; stir at room temperature, and perform ultrasound with an ultrasound frequency of 40 kHz, an ultrasound power of 200 w, and an ultrasound time of 5 min; until the p-hydroxyacetophenone is completely dissolved to obtain sample 1;

[0102] b. Accurately weigh cedar alcohol and add it to sample 1; stir at room temperature, assisted by ultrasound, the ultrasonic frequency is 40kHz, the ultrasonic power is 200w, and the ultrasonic time is 10min; until the cedar alcohol is completely dissolved to obtain sample 2;

[0103] c. Accurately weigh glycerol and add it to sample b; stir at room temperature, assisted by ultrasound, the ultrasonic frequency is 40kHz, the ultrasonic power is 200w, and the ultrasonic time is 10min; until the sample is mixed evenly, and sample 3 is obtained;

[0104] d. Accurately weigh the Platycladus orientalis leaf extract solution and add it to sample 3 to make the mass fraction of Platycladus orientalis leaf extract be 0.1%, stir at room temperature until the sample is evenly mixed to obtain a cedar alcohol hair growth liquid composition.

[0105] Comparative Example 4

[0106] A cedar alcohol hair growth liquid composition, comprising, by weight: 2-methyl-1,3-propanediol: 1,3-butanediol: 2-methyl-2,4-pentanediol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = 33.57: 41.03: 18.65: 0.50: 0.50: 4.96: 0.80, with a net content of Platycladus orientalis leaf extract of 0.2%; the viscosity of the obtained cedar alcohol hair growth liquid composition is 107.33 cP, the cedar alcohol drug loading is 5%, and the net content of Platycladus orientalis leaf extract is 0.2%;

[0107] Specifically: 3.36 g of 2-methyl-1,3-propanediol, 4.10 g of 1,3-butanediol, 1.86 g of 2-methyl-2,4-pentanediol, 0.05 g of 1,2-hexanediol, 0.05 g of p-hydroxyacetophenone, 0.50 g of cedar alcohol, and 0.08 g of Platycladus orientalis leaf extract solution.

[0108] The preparation method of the cedar alcohol hair growth liquid composition comprises the following steps:

[0109] a. Accurately weigh 2-methyl-1,3-propanediol, 1,3-butanediol, 2-methyl-2,4-pentanediol, 1,2-hexanediol, and p-hydroxyacetophenone and mix them; stir at room temperature, assisted by ultrasound, with an ultrasound frequency of 40 kHz, an ultrasound power of 200 w, and an ultrasound time of 5 min; until the p-hydroxyacetophenone is completely dissolved to obtain sample 1;

[0110] b. Accurately weigh cedar alcohol and add it to sample 1; stir at room temperature, assisted by ultrasound, the ultrasonic frequency is 40kHz, the ultrasonic power is 200w, and the ultrasonic time is 10min; until the cedar alcohol is completely dissolved to obtain sample 2;

[0111] c. Accurately weigh the Platycladus orientalis leaf extract solution and add it to sample 2 to make the mass fraction of Platycladus orientalis leaf extract be 0.2%, stir at room temperature until the sample is evenly mixed to obtain a cedar alcohol hair growth liquid composition.

[0112] Application example: Evaluation of the hair growth promoting and hair loss preventing regulating effect of the cedar alcohol hair growth liquid composition prepared in the example

[0113] 1. Animals:

[0114] C57BL / 6 mice, male, 18-22 g, were purchased from Liaoning Changsheng Animal Co., Ltd., certificate number: SCXK (Liao) 2015-0001.

[0115] 2. Drugs and reagents:

[0116] (1) Cedrol: white crystals; Liaoning Xinzhong Modern Pharmaceutical Co., Ltd., purity > 98%; structural formula: Figure 1 ;

[0117] (2) Minoxidil tincture: Zhejiang Wansheng Pharmaceutical Co., Ltd., national medicine approval number H20010714;

[0118] (3) 95% ethanol: Tianjin Damao Chemical Reagent Factory;

[0119] (4) 10% paraformaldehyde: Wuhan Saiweier Biological Co., Ltd.;

[0120] (5) Depilatory cream: Veet depilatory cream available on the market;

[0121] (6) Sodium carboxymethyl cellulose (CMC-Na): Aladdin Chemical Reagent Co., Ltd. (C304951);

[0122] (7) Cyclophosphamide: Baxter Oncology Gmbh, specification: 0.2 g.

[0123] 3. Methods:

[0124] The cyclophosphamide (CP)-induced hair loss model was used to evaluate the hair growth effect of different dosing samples.

[0125] Establishment of hair loss model: 48 male C57BL / 6 mice (Liaoning Changsheng Animal Co., Ltd.) were randomly divided into 6 groups (8 mice in each group): control group, model group, Example 2, Example 3, and 2% minoxidil tincture (MD) group, a total of 5 groups. After purchase, they were raised normally for 7 days. After the end, the mice were intraperitoneally injected with 4% chloral hydrate for mouse anesthesia. Gently lift the mouse's limbs and turn the mouse over. If the mouse does not resist, it means that the mouse is anesthetized successfully. Use a small animal hair preparer to cut a 2*3 cm thick hair on the back of the mouse. 2 The area is prepared for skin preparation. After the skin preparation is completed, a mild depilatory cream is applied to the prepared area. After standing for 2 minutes, a non-damaging plastic scraper is used to gently scrape the depilatory cream, and then a wet wipe is used to pick it up until the depilatory cream on the back of the mouse is completely removed. Then an image capture device is used to capture images of the mouse's back. On the first day after depilation, images of the mouse's back are captured during the mouse's special hair cycle. The topical application is once a day, Example 2 (0.1 mL / mouse), Example 3 (0.1 mL / mouse), 2% minoxidil tincture (0.1 mL / mouse), the model group and the control group do not receive any treatment, and the application is continued for 28 days. On the 9th day during the administration (drug application), mice in other groups except the control group are intraperitoneally injected with 150 mg / kg of cyclophosphamide. After the experiment, the hair length and hair weight ( Figure 2 ).

[0126] 4. Results:

[0127] (1) Effects of different samples on cyclophosphamide-induced alopecia in mice

[0128] Examples 2 and 3 both significantly promoted hair growth in mouse skin tissue, effectively prevented CP-induced hair loss, and promoted hair regrowth in hair-loss mice, which was superior to the 2% minoxidil (MD) group ( Figure 3 ).

[0129] (2) Effects of different samples on mouse hair length and weight

[0130] After the last administration, the hair length and weight of the hair loss area on the back of the mice were measured. It was found that the hair length and weight of Example 3 were the best, better than Example 2 and the 2% minoxidil tincture (MD) group.

[0131] Table 7 Effects of different cedar alcohol administration methods on hair length and weight in CP-induced alopecia areata mice (28 days)

[0132] Group Hair length (mm) Hair weight (mg) Control 8.13±0.64 53.02±9.69 Model 4.04±0.59 17.27±4.76 Example 2 4.72±0.65 22.68±4.61 Example 3 6.17±0.52 37.86±4.69 MD2% 5.15±0.72 27.34±5.62

[0133] The present invention adopts two extreme vertex designs to deeply optimize the formula of the cedar alcohol and Platycladus orientalis leaf extract composition with hair growth effect, and takes viscosity as the core evaluation index to obtain a uniform, stable, yellow, transparent cedar alcohol and Platycladus orientalis leaf extract hair growth liquid; the viscosity of the cedar alcohol and Platycladus orientalis leaf extract hair growth liquid is moderate (above 200cP), wherein the drug loading amount of cedar alcohol is 0.5-10%, and the net content of Platycladus orientalis leaf extract is 0.1-2%, which lays a foundation for the use of the cedar alcohol and Platycladus orientalis leaf extract composition for external use on the head skin to promote hair growth.

Claims

1. A cedar alcohol hair growth liquid composition, characterized in that: The composition comprises cedrol and orientalis leaf extract.

2. A cedar alcohol hair growth liquid composition according to claim 1, characterized in that: Its composition includes, by weight ratio: 2-methyl-1,3-propanediol: 1,3-butanediol: 2-methyl-2,4-pentanediol: glycerol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = (18-25): (25-31): (7-10): (20-29): (0.2-0.8): (0.2-0.8): (4-15): (0.8-15).

3. The cedar alcohol hair growth liquid composition according to claim 1, characterized in that: The viscosity of the cedar alcohol hair growth liquid composition is above 200 cP, the drug loading amount of cedar alcohol is 0.5% to 10%, and the net content of Platycladus orientalis leaf extract in the cedar alcohol hair growth liquid composition is 0.1% to 2%.

4. The cedar alcohol hair growth liquid composition according to claim 3, characterized in that: The drug loading amount of cedar alcohol is 10%, and the net content of Platycladus orientalis leaf extract in the cedar alcohol hair growth liquid composition is 2%.

5. The cedar alcohol hair growth liquid composition according to claim 2, characterized in that: Its composition includes, by weight ratio: 2-methyl-1,3-propanediol: 1,3-butanediol: 2-methyl-2,4-pentanediol: glycerol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = (18-22): (26-29): (8-9): (23-25): 0.50: 0.50: 10.00: (8-9).

6. The cedar alcohol hair growth liquid composition according to claim 5, characterized in that: Its composition is calculated by weight ratio: 2-methyl-1,3-propanediol: 1,3-butanediol: 2-methyl-2,4-pentanediol: glycerol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = 22.00: 26.50: 9.00: 23.50: 0.50: 0.50: 10.00: 8.

00.

7. The cedar alcohol hair growth liquid composition according to claim 5, characterized in that: Its composition is calculated by weight ratio: 2-methyl-1,3-propanediol: 1,3-butanediol: 2-methyl-2,4-pentanediol: glycerol: 1,2-hexanediol: p-hydroxyacetophenone: cedar alcohol: Platycladus orientalis leaf extract solution = 18.00: 29.00: 8.81: 25.00: 0.50: 0.50: 10.00: 8.

19.

8. A method for preparing the cedar alcohol hair growth liquid composition according to claim 2, characterized in that: The following steps are involved: a. Accurately weigh 2-methyl-1,3-propanediol, 1,3-butanediol, 2-methyl-2,4-pentanediol, 1,2-hexanediol, and p-hydroxyacetophenone and mix them; stir at room temperature, assisted by ultrasound, or stir at 45°C until p-hydroxyacetophenone is completely dissolved to obtain sample 1; b. Accurately weigh cedar alcohol and add it to sample 1; stir at room temperature, assisted by ultrasound, or stir at 45°C until the cedar alcohol is completely dissolved to obtain sample 2; c. Accurately weigh glycerol and add it to sample 2; stir at room temperature, with the aid of ultrasound, or stir at 45°C until the sample is evenly mixed to obtain sample 3; d. Accurately weigh the Platycladus orientalis leaf extract solution and add it to sample 3, stir at room temperature until the sample is evenly mixed to obtain a cedar alcohol hair growth liquid composition with a viscosity of more than 200 cP.

9. The method for preparing a cedar alcohol hair growth liquid composition according to claim 8, characterized in that: The ultrasound in step a has a frequency of 40kHz, a power of 200W, and an ultrasound time of 5min; the ultrasound in step b has a frequency of 40kHz, a power of 200W, and an ultrasound time of 5min; the ultrasound in step c has a frequency of 40kHz, a power of 200W, and an ultrasound time of 10min; the net content of the added Platycladus orientalis leaf extract in the cedar alcohol hair growth liquid composition is 0.1-2%.

10. Use of the cedar alcohol hair growth liquid composition according to any one of claims 1 to 7 in the preparation of cosmetics for promoting hair growth on the head.

Images