Fermented traditional Chinese medicine preparation for treating trichomoniasis of poultry and preparation method of fermented traditional Chinese medicine preparation

Abstract

The invention provides a fermented traditional Chinese medicine preparation for treating trichomoniasis of poultry and a preparation method of the fermented traditional Chinese medicine preparation, and relates to the technical field of traditional Chinese medicine fermented preparations for livestock and poultry. The invention provides a fermented traditional Chinese medicine preparation prepared from a traditional Chinese medicine composition and compound probiotics on the basis of the traditional Chinese veterinary dialectical theory according to the pathogenesis characteristics and the treatment principle of poultry trichomoniasis. Wherein the traditional Chinese medicine composition is prepared from senecio scandens, juncus roemerianus, felwort, trollflower, fructus gardeniae praeparatus, pollen typhae, trogopterus dung, semen pharbitidis, folium mori, tripterygium wilfordii, notopterygium root, rhizoma atractylodis and agastache rugosus, and has the effects of clearing away heat and toxic materials, cooling blood and relieving pain, killing parasites and removing food retention, and clearing damp and tonifying spleen; after the traditional Chinese medicine composition is subjected to probiotic fermentation, the treatment and prevention effects of the traditional Chinese medicine composition on trichomonad of poultry can be remarkably improved. The fermented traditional Chinese medicine preparation disclosed by the invention has obvious killing and preventing effects on trichomonad in vivo and in vitro, the death rate is reduced, the body weight of a diseased body is quickly recovered, and the infection rate of poultry trichomonad is obviously reduced; meanwhile, the fermented traditional Chinese medicine preparation disclosed by the invention has excellent anti-inflammatory and anti-inflammatory effects, and has repairing and preventing effects on liver injury caused by trichomonas infection. The invention provides a new medicine for treating and preventing the trichomonad of poultry.

Description

Technical Field

[0001] The invention relates to the technical field of fermented Chinese medicinal preparations for livestock and poultry, and in particular to a fermented Chinese medicinal preparation for treating avian Trichomonas and a preparation method thereof. Background Art

[0002] Avian trichomoniasis is a protozoan disease caused by Trichomonas, characterized by ulcers and diarrhea in the upper digestive tract of birds. It is transmitted through contaminated feed and drinking water. Turkeys, chickens, ducks, geese, pigeons and eagles are susceptible and young birds are seriously ill. Avian trichomoniasis is characterized by cheesy accumulation in the throat, often accompanied by weight loss. Birds with upper digestive tract infection show loss of appetite, mental depression, collapsed crop, neck often stretched as if swallowing, watery eyes, difficulty closing the mouth, light green to light yellow mucus flowing out of the mouth, and emitting a foul odor; birds with lower digestive tract infection show depression, loss of appetite or loss of appetite, ruffled feathers, unstable gait, standing or lying down, light yellow watery feces, weight loss, lethargy, and even death. Avian Trichomonas invades the mucosal surface of the mouth, nasal cavity, pharynx, esophagus and crop.

[0003] Breeding sites need to strictly implement environmental sanitation and disinfection systems, such as using fumigation, exposure to the sun and other methods to disinfect the environment, promptly clean up ground feces to cut off the transmission route and source of infection, and change from flat breeding to cage breeding. In addition to the above-mentioned physical means, drug treatment is more commonly used in the prevention and control of avian trichomoniasis. Nitroimidazole drugs have been favored by people since their advent, and metronidazole is one of the best. However, metronidazole has been used for a long time, and its drug resistance problem has also become prominent. Relevant departments in my country have issued specific regulations prohibiting the use of metronidazole in the production of animal-derived foods. The remaining nitroimidazole drugs belong to the same class of drugs as metronidazole, and also have the risk of carcinogenicity and mutagenicity.

[0004] In view of the practical problems in the prevention and treatment of poultry trichomoniasis, traditional Chinese veterinary medicine has brought new hope for solving the above problems. Chinese medicine has many advantages: it is not easy to cause drug resistance, has very little toxic side effects, and no residue has been found so far. At present, there are few reports on the use of Chinese medicine preparations for the prevention and treatment of poultry trichomoniasis. It is very necessary to provide a Chinese medicine preparation that can effectively prevent and treat poultry trichomoniasis and enhance the immunity of poultry. Summary of the invention

[0005] 1. Technical issues to be solved

[0006] In view of the deficiencies in the prior art, the present invention provides a fermented Chinese medicine preparation for treating avian trichomoniasis and a preparation method thereof.

[0007] (II) Technical solution

[0008] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0009] The invention provides a fermented Chinese medicine preparation for treating avian trichomonas. The preparation is prepared from a Chinese medicine composition and composite probiotics, wherein the Chinese medicine composition is composed of the following raw materials in parts by weight: 10-30 parts of Senecio radix, 10-30 parts of Juncus effusus, 10-30 parts of Gentiana scabra, 5-20 parts of Trollius chinensis, 5-20 parts of Gardenia jasminoides, 5-20 parts of Pollen Typhae, 5-20 parts of Ophiopogon japonicus, 10-30 parts of Pharbitidis seeds, 10-30 parts of Morus alba, 10-30 parts of Tripterygium wilfordii, 5-20 parts of Notopterygium wilfordii, 5-20 parts of Atractylodes macrocephala, and 5-20 parts of Patchouli rugosa; and the composite probiotics are composed of Lactobacillus plantarum, Enterococcus faecalis, and Saccharomyces cerevisiae in the following weight ratio of 1-2:0.5-1:0.5-1.

[0010] Furthermore, the Chinese medicine composition is composed of the following raw materials in parts by weight: 15-25 parts of Senecio, 20-30 parts of Juncus effusus, 15-25 parts of Gentiana scabra, 10-20 parts of Nasturtium, 5-15 parts of Gardenia jasminoides, 5-15 parts of Pollen Typhae, 5-15 parts of Ophiopogon japonicus, 15-25 parts of Pharbitis chinensis, 20-30 parts of mulberry leaves, 15-25 parts of Tripterygium wilfordii, 5-15 parts of Notopterygium wilfordii, 5-15 parts of Atractylodes lancea, and 5-10 parts of Patchouli; the composite probiotics are composed of Lactobacillus plantarum, Enterococcus faecalis, and Saccharomyces cerevisiae in the following weight ratio of 1-1.6:0.5-0.8:0.5-0.8.

[0011] Furthermore, the Chinese medicine composition is composed of the following raw materials in parts by weight: 22 parts of Senecio, 26 parts of Juncus effusus, 20 parts of Gentiana scabra, 16 parts of Nasturtium, 12 parts of Gardenia jasminoides, 8 parts of Pollen Typhae, 10 parts of Ophiopogon japonicus, 22 parts of Pharbitis chinensis, 24 parts of Morus alba, 20 parts of Tripterygium wilfordii, 12 parts of Notopterygium wilfordii, 10 parts of Atractylodes macrocephala, and 10 parts of Patchouli rugosa; the composite probiotics are composed of Lactobacillus plantarum, Enterococcus faecalis, and Saccharomyces cerevisiae in the following weight ratio of 1.4:0.7:0.5.

[0012] Furthermore, the number of viable bacteria of each probiotic is: Lactobacillus plantarum ≥ 1.5×10 10 CFU / g, Enterococcus faecalis ≥9×10 9 CFU / g, brewer's yeast ≥9×10 8 CFU / g.

[0013] The present invention also provides a method for preparing a fermented Chinese medicine preparation for treating avian Trichomonas. The fermented Chinese medicine preparation is prepared according to the following steps:

[0014] (1) Weighing Senecio radix, Juncus wickerensis, Gentiana scabra, Nasturtium, Gardenia jasminoides, Pollen Typhae, Ophiopogon japonicus, Pharbitis chinensis, Morus alba, Tripterygium wilfordii, Notopterygium wilfordii, Atractylodes lancea, and Patchouli chinensis according to the above weight proportions, washing, drying, crushing, and passing through a 100-mesh sieve to obtain a fermentation raw material for later use;

[0015] (2) weighing Lactobacillus plantarum, Enterococcus faecalis, and Saccharomyces cerevisiae according to the above weight portions to obtain a composite probiotic powder, and then mixing 10-20% of the composite probiotic powder, 5-10% of glucose, 5-10% of cellulase, and 60-80% of purified water, stirring well, and culturing in a closed manner at 37° C. for 24 hours to obtain an activated bacterial solution for standby use;

[0016] (3) The fermentation raw material obtained in step (1) is uniformly mixed with 0.2-0.5 times of purified water, and then uniformly mixed with the activated bacterial solution obtained in step (2), and then placed in a sealed container for anaerobic sealed fermentation at a fermentation temperature of 30-40° C., a fermentation humidity of 30-40%, and a fermentation time of 3-5 days to obtain the fermented Chinese medicine preparation.

[0017] The invention provides a fermented Chinese medicine preparation for preparing a medicine for treating avian trichomoniasis. The fermented Chinese medicine preparation can be prepared into any oral dosage form acceptable to animals according to conventional methods, and the dosage form includes oral liquid, lyophilized powder, granules, tablets and pills.

[0018] Senecio: cold in nature, bitter in taste, has the effects of clearing away heat and detoxifying, improving eyesight, and relieving itching. It is mostly used for wind-heat colds, red and swollen eyes, diarrhea, dysentery, skin eczema, sores and boils.

[0019] Juffure: sweet and light in taste, slightly cold in nature, enters the heart, lung, and small intestine meridians; clears heart fire and promotes urination, used for restlessness and insomnia, less urinary pain, and mouth and tongue ulcers.

[0020] Gentiana scabra: bitter in taste, cold in nature, enters the liver and gallbladder meridians; clears away heat and dampness, relieves liver and gallbladder fire, used for damp-heat jaundice, vaginal swelling and itching, leucorrhea, eczema and itching, red eyes due to liver fire, tinnitus and deafness, pain in the ribs and bitter taste in the mouth, strong middle wind, and convulsions.

[0021] Golden lotus: bitter, cold, non-toxic; functions and indications: clearing heat and detoxifying. Treats upper respiratory tract infection, tonsillitis, pharyngitis, acute otitis media, acute tympanitis, acute conjunctivitis, acute lymphangitis, mouth sores, furunculosis. ① "Shanhai Caohan": Treats furunculosis, all kinds of wind. ② "Gangmu Shiyi": Treats mouth sores, sore throat, superficial heat, toothache, earache, eye pain, improves eyesight, and relieves miasma. ③ "Hebei Traditional Chinese Medicine Handbook": Clears heat and detoxifies

[0022] Scorched Gardenia: bitter in taste, cold in nature. Enters the heart, lung and triple burner meridians; used for vomiting and bleeding due to blood heat, hematuria and metrorrhagia.

[0023] Puhuang: sweet, neutral, enters the liver and pericardium meridians, stops bleeding, removes blood stasis, and relieves stranguria; used for hematemesis, epistaxis, hemoptysis, metrorrhagia, bleeding from trauma, amenorrhea, stabbing pain in the chest and abdomen, swelling and pain from falls, and painful stranguria with blood.

[0024] Wulingzhi: sweet, warm, belongs to the liver meridian; promotes blood circulation and relieves pain, removes blood stasis and stops bleeding; used raw to promote blood circulation and stop pain. Treats various pains caused by blood and qi in the heart and abdomen, amenorrhea in women, and pain caused by blood stasis after childbirth; externally treats bites by snakes, scorpions, and centipedes. Stir-fried to stop bleeding.

[0025] Pharbitis seeds: bitter, cold in nature, slightly toxic. Has the effects of purging water, lowering qi, and expelling parasites. Used for edema, wheezing, phlegm, beriberi, parasite accumulation, constipation, etc.

[0026] Mulberry leaf: bitter and sweet in taste, cold in nature, enters the lung and liver meridians; dispels wind-heat, clears the lungs and moistens dryness, soothes the liver and improves eyesight, cools blood and stops bleeding; used for wind-heat colds, the initial stage of febrile disease, lung heat cough, dizziness caused by hyperactivity of liver yang, red and blurred vision, and coughing up and vomiting blood due to blood heat.

[0027] Tripterygium wilfordii: bitter, highly toxic, dispels wind and dampness; promotes blood circulation and dredges the meridians; reduces swelling and relieves pain; kills insects and detoxifies.

[0028] Qianghuo: warm in nature, it can relieve exterior symptoms, dispel cold and dampness, and is used for exogenous wind-cold, headache without sweating, cold-dampness arthralgia, and rheumatic pain in the upper limbs.

[0029] Atractylodes: pungent and bitter in taste, warm in nature, enters the spleen, stomach, and liver meridians; dries dampness and strengthens the spleen, dispels wind and cold, and improves eyesight; used for dampness blocking the middle burner, abdominal distension, diarrhea, edema, beriberi, rheumatic arthralgia, colds, night blindness, and blurred vision.

[0030] Patchouli: spicy in flavor, warm in nature, enters the spleen, stomach, and lung meridians; eliminates dampness and invigorates the spleen, dispels filth and harmonizes the middle, relieves heat, and releases the exterior; used for dampness blocking the spleen and stomach, abdominal distension, initial onset of damp-heat, vomiting, diarrhea, summer-dampness, fever and chills, fever and chills, chest and abdominal fullness and stuffiness, etc.

[0031] (III) Beneficial effects

[0032] The present invention is based on the theory of traditional Chinese veterinary syndrome differentiation, and provides a fermented Chinese medicine preparation prepared by a Chinese medicine composition and a composite probiotic for the pathogenesis characteristics and treatment principles of avian trichomoniasis, wherein the Chinese medicine composition is composed of Senecio radix, Juncus juncis, Gentiana scabra, Nasturtium, Gardenia jasminoides, Pollen Typhae, Rhizoma Atractylodis Macrocephalae, Semen Pharbitidis, Folium Mori, Tripterygium wilfordii, Notopterygium incisifolia, Atractylodes macrocephala, and Patchouli, and has the effects of clearing away heat and detoxifying, cooling blood and relieving pain, killing insects and eliminating accumulation, and removing dampness and strengthening the spleen. The above-mentioned Chinese medicine composition can significantly improve its therapeutic and preventive effects on avian trichomoniasis after probiotic fermentation. The fermented Chinese medicine preparation of the present invention has a significant killing effect on trichomoniasis both in vivo and in vitro, can avoid the occurrence of death of sick young pigeons, and quickly restore the body weight of the sick body; at the same time, the fermented Chinese medicine preparation of the present invention has excellent anti-inflammatory and anti-inflammatory effects, and has a repair and preventive effect on liver damage caused by trichomoniasis infection. The fermented Chinese medicinal preparation of the invention also has a good preventive effect on Trichomonas, can significantly reduce the infection rate of Trichomonas in pigeons, and provides a new medicine for the treatment and prevention of Trichomonas in birds. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 The weights of young pigeons in each group before and after medication; within each group, after medication compared with before medication, *P<0.05, ***P<0.001; before medication, each diseased group compared with the prevention group, ###P<0.001.

[0034] Figure 2 The numbers of Trichomonas in young pigeons of each group on the 0th, 1st, 3rd and 7th days of the experiment; within each group, compared with the 0th day, ***P<0.001, ****P<0.0001.

[0035] Figure 3 The numbers of Trichomonas in young pigeons in the fermented Chinese medicine preparation group, Chinese medicine control group and Western medicine control group on the 1st, 3rd and 7th days of the experiment; on the same day, compared with the fermented Chinese medicine preparation group, *P<0.05, ***P<0.01, ***P<0.001.

[0036] Figure 4 The levels of inflammatory factors IL-6 and IL-1β in the serum of young pigeons in each group after administration; compared with the positive control group, ****P<0.0001; compared with the fermented Chinese medicine preparation group, *P<0.05, ***P<0.01.

[0037] Figure 5 ALT and AST levels in serum of young pigeons in each group after administration; **P<0.01 compared with the positive control group. DETAILED DESCRIPTION

[0038] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] Example 1

[0040] A fermented Chinese medicine preparation for treating avian Trichomonas is prepared according to the following steps:

[0041] (1) Weigh the following raw materials in parts by weight: 10 parts of Senecio grandiflora, 10 parts of Juncus effusus, 10 parts of Gentiana scabra, 5 parts of Nasturtium, 5 parts of Gardenia jasminoides, 5 parts of Pollen Typhae, 5 parts of Ophiopogon japonicus, 10 parts of Pharbitidis seeds, 10 parts of Morus alba, 10 parts of Tripterygium wilfordii, 5 parts of Notopterygium wilfordii, 5 parts of Atractylodes lancea, and 5 parts of Patchouli grandiflora, wash, dry, crush, and pass through a 100-mesh sieve to obtain a fermentation raw material for later use;

[0042] (2) Weigh each probiotic powder according to the following weight ratio: Lactobacillus plantarum, Enterococcus faecalis, and Saccharomyces cerevisiae in a ratio of 1:0.5:0.5, and mix them evenly to obtain a composite probiotic powder; then, add 15% composite probiotic powder, 8% glucose, 5% cellulase, and 72% purified water, mix and stir them evenly, and culture them in a closed environment at 37°C for 24 hours to obtain an activated bacterial solution for use; the number of viable bacteria of each probiotic is: Lactobacillus plantarum ≥ 1.5 × 10 10 CFU / g, Enterococcus faecalis ≥9×10 9 CFU / g, brewer's yeast ≥9×10 8 CFU / g;

[0043] (3) The fermentation raw material obtained in step (1) is uniformly mixed with 0.3 times purified water, and then uniformly mixed with the activated bacterial solution obtained in step (2), and then placed in a sealed container for anaerobic sealed fermentation at a fermentation temperature of 37° C., a fermentation humidity of 30-40%, and a fermentation time of 4 days to obtain the fermented Chinese medicine preparation.

[0044] Example 2

[0045] The difference between this embodiment and embodiment 1 is that:

[0046] The Chinese medicine composition is composed of the following raw materials in parts by weight: 30 parts of Senecio radix, 30 parts of Juncus effusus, 30 parts of Gentiana scabra, 20 parts of Nasturtium, 20 parts of Gardenia jasminoides, 20 parts of Pollen Typhae, 20 parts of Ophiopogonis aconitifolia, 30 parts of Pharbitidis seeds, 30 parts of Morus alba, 30 parts of Tripterygium wilfordii, 20 parts of Notopterygium wilfordii, 20 parts of Atractylodes macrocephala, and 20 parts of Patchouli;

[0047] The composite probiotics are composed of Lactobacillus plantarum, Enterococcus faecalis and Saccharomyces cerevisiae in the following weight ratio of 2:01:1.

[0048] Example 3

[0049] The difference between this embodiment and embodiment 1 is that:

[0050] The Chinese medicine composition is composed of the following raw materials in parts by weight: 14 parts of Senecio radix, 16 parts of Juncus effusus, 12 parts of Gentiana scabra, 8 parts of Nasturtium, 10 parts of Gardenia jasminoides, 8 parts of Pollen Typhae, 8 parts of Ophiopogonis aconitifolia, 16 parts of Pharbitidis seeds, 14 parts of Morus alba, 12 parts of Tripterygium wilfordii, 6 parts of Notopterygium wilfordii, 8 parts of Atractylodes lancea, and 8 parts of Patchouli;

[0051] The composite probiotics are composed of plant lactobacillus, enterococcus faecalis and brewer's yeast in the following weight ratio of 1.2:0.6:0.5.

[0052] Example 4

[0053] The difference between this embodiment and embodiment 1 is that:

[0054] The Chinese medicine composition is composed of the following raw materials in parts by weight: 28 parts of Senecio radix, 26 parts of Juncus effusus, 24 parts of Gentiana scabra, 16 parts of Nasturtium, 14 parts of Gardenia jasminoides, 18 parts of Pollen Typhae, 14 parts of Ophiopogonis aconitifolia, 26 parts of Pharbitidis seeds, 26 parts of Morus alba, 24 parts of Tripterygium wilfordii, 12 parts of Notopterygium wilfordii, 10 parts of Atractylodes lancea, and 12 parts of Patchouli;

[0055] The composite probiotics are composed of plant lactobacillus, enterococcus faecalis and brewer's yeast in the following weight ratio of 1.5:0.7:0.6.

[0056] Example 5

[0057] The difference between this embodiment and embodiment 1 is that:

[0058] The Chinese medicine composition is composed of the following raw materials in parts by weight: 28 parts of Senecio radix, 26 parts of Juncus effusus, 24 parts of Gentiana scabra, 16 parts of Nasturtium, 14 parts of Gardenia jasminoides, 18 parts of Pollen Typhae, 14 parts of Ophiopogonis aconitifolia, 26 parts of Pharbitidis seeds, 26 parts of Morus alba, 24 parts of Tripterygium wilfordii, 12 parts of Notopterygium wilfordii, 10 parts of Atractylodes lancea, and 12 parts of Patchouli;

[0059] The composite probiotics are composed of plant lactobacillus, enterococcus faecalis and brewer's yeast in the following weight ratio of 1.4:0.7:0.5.

[0060] Example 6

[0061] The difference between this embodiment and embodiment 1 is that:

[0062] The Chinese medicine composition is composed of the following raw materials in parts by weight: 15 parts of Senecio radix, 20 parts of Juncus effusus, 15 parts of Gentiana scabra, 10 parts of Nasturtium, 5 parts of Gardenia jasminoides, 5 parts of Pollen Typhae, 5 parts of Ophiopogonis aconitifolia, 15 parts of Pharbitidis seeds, 20 parts of Morus alba, 15 parts of Tripterygium wilfordii, 5 parts of Notopterygium wilfordii, 5 parts of Atractylodes macrocephala, and 5 parts of Patchouli grandiflora;

[0063] The composite probiotics are composed of Lactobacillus plantarum, Enterococcus faecalis and Saccharomyces cerevisiae in the following weight ratio of 1:0.5:0.5.

[0064] Example 7

[0065] The difference between this embodiment and embodiment 1 is that:

[0066] The Chinese medicine composition is composed of the following raw materials in parts by weight: 25 parts of Senecio radix, 30 parts of Juncus effusus, 25 parts of Gentiana scabra, 20 parts of Nasturtium, 15 parts of Gardenia jasminoides, 15 parts of Pollen Typhae, 15 parts of Ophiopogonis aconitifolia, 25 parts of Pharbitidis seeds, 30 parts of Morus alba, 25 parts of Tripterygium wilfordii, 15 parts of Notopterygium wilfordii, 15 parts of Atractylodes lancea, and 10 parts of Patchouli;

[0067] The composite probiotics are composed of Lactobacillus plantarum, Enterococcus faecalis and Saccharomyces cerevisiae in the following weight ratio of 1.6:0.8:0.8.

[0068] Example 8

[0069] The difference between this embodiment and embodiment 1 is that:

[0070] The Chinese medicine composition is composed of the following raw materials in parts by weight: 18 parts of Senecio radix, 22 parts of Juncus effusus, 20 parts of Gentiana scabra, 12 parts of Nasturtium, 10 parts of Gardenia jasminoides, 6 parts of Pollen Typhae, 8 parts of Ophiopogonis aconitifolia, 20 parts of Pharbitidis seeds, 22 parts of Morus alba, 16 parts of Tripterygium wilfordii, 8 parts of Notopterygium wilfordii, 8 parts of Atractylodes lancea, and 8 parts of Patchouli grandiflora;

[0071] The composite probiotics are composed of Lactobacillus plantarum, Enterococcus faecalis and Saccharomyces cerevisiae in the following weight ratio of 1.4:0.7:0.5.

[0072] Example 9

[0073] The difference between this embodiment and embodiment 1 is that:

[0074] The Chinese medicine composition is composed of the following raw materials in parts by weight: 20 parts of Senecio radix, 20 parts of Juncus effusus, 20 parts of Gentiana scabra, 14 parts of Nasturtium, 8 parts of Gardenia jasminoides, 8 parts of Pollen Typhae, 8 parts of Ophiopogonis atractylodes, 20 parts of Pharbitidis seeds, 20 parts of Morus alba leaves, 20 parts of Tripterygium wilfordii, 8 parts of Notopterygium wilfordii, 8 parts of Atractylodes lancea, and 8 parts of Patchouli;

[0075] The composite probiotics are composed of Lactobacillus plantarum, Enterococcus faecalis and Saccharomyces cerevisiae in the following weight ratio of 1.4:0.7:0.5.

[0076] Example 10

[0077] The difference between this embodiment and embodiment 1 is that:

[0078] The Chinese medicine composition is composed of the following raw materials in parts by weight: 24 parts of Senecio radix, 28 parts of Juncus effusus, 18 parts of Gentiana scabra, 18 parts of Nasturtium, 10 parts of Gardenia jasminoides, 10 parts of Pollen Typhae, 10 parts of Ophiopogonis aconitifolia, 20 parts of Pharbitidis seeds, 28 parts of Morus alba, 18 parts of Tripterygium wilfordii, 10 parts of Notopterygium wilfordii, 10 parts of Atractylodes macrocephala, and 10 parts of Patchouli grandiflora;

[0079] The composite probiotics are composed of Lactobacillus plantarum, Enterococcus faecalis and Saccharomyces cerevisiae in the following weight ratio of 1.4:0.7:0.5.

[0080] Embodiment 11

[0081] The difference between this embodiment and embodiment 1 is that:

[0082] The Chinese medicine composition is composed of the following raw materials in parts by weight: 22 parts of Senecio, 26 parts of Juncus effusus, 20 parts of Gentiana scabra, 16 parts of Nasturtium, 12 parts of Gardenia jasminoides, 8 parts of Pollen Typhae, 10 parts of Ophiopogon japonicus, 22 parts of Pharbitis chinensis, 24 parts of Morus alba, 20 parts of Tripterygium wilfordii, 12 parts of Notopterygium wilfordii, 10 parts of Atractylodes macrocephala, and 10 parts of Patchouli rugosa; the composite probiotics are composed of Lactobacillus plantarum, Enterococcus faecalis, and Saccharomyces cerevisiae in the following weight ratio of 1.4:0.7:0.5.

[0083] Test Example 1

[0084] Study on the effect of the Chinese medicine composition in the fermented Chinese medicine preparation of the present invention on anti-pigeon trichomonas in vitro

[0085] 1. Drug preparation

[0086] 1.1 Preparation of liver infusion medium

[0087] Take 15g of fresh pork liver, cut it into rice-sized pieces, add appropriate amount of pure water and place it in a 4℃ refrigerator overnight. The next day, boil the pork liver into liver soup, filter it with double-layer gauze, and take the supernatant. Add appropriate amount of L-cysteine, peptone, and sodium chloride to the supernatant, make up to 100mL with pure water, adjust the pH to 5-6, sterilize it at 8 pounds for 20 minutes, and store it in a 4℃ refrigerator for later use. Add 20% sterilized calf serum, 10μg / mL amphotericin B, and 100U / mL each of penicillin and streptomycin before use.

[0088] 1.2 Preparation of test drugs

[0089] Drug 1: 22 parts of Senecio, 26 parts of Juncus, 20 parts of Gentiana, 16 parts of Nasturtium, 12 parts of Gardenia, 8 parts of Pollen Typhae, 10 parts of Ophiopogonis, 22 parts of Pharbitis Seed, 24 parts of Morus Alata, 20 parts of Tripterygium, 12 parts of Notopterygium, 10 parts of Atractylodes, 10 parts of Patchouli

[0090] Drug 2: 20 parts of Gentiana, 16 parts of Nasturtium, 12 parts of Gardenia, 8 parts of Pollen, 10 parts of Ophiopogon japonicus, 22 parts of Pharbitidis, 20 parts of Tripterygium wilfordii, 12 parts of Notopterygium wilfordii, 10 parts of Atractylodes macrocephala, 10 parts of Patchouli

[0091] Drug 3: 22 parts of Senecio, 26 parts of Juncus, 12 parts of Gardenia, 8 parts of Pollen Typhae, 10 parts of Ophiopogonis, 24 parts of Morus alba, 12 parts of Notopterygium, 10 parts of Atractylodes, 10 parts of Patchouli

[0092] Drug 4: 22 parts of Senecio, 26 parts of Juncus, 20 parts of Gentiana, 16 parts of Nasturtium, 22 parts of Pharbitis seeds, 24 parts of Morus alba, 20 parts of Tripterygium wilfordii, 12 parts of Notopterygium wilfordii, 10 parts of Atractylodes, 10 parts of Patchouli

[0093] Drug 5: 22 parts of Senecio, 26 parts of Juncus, 20 parts of Gentiana, 16 parts of Nasturtium, 12 parts of Gardenia, 8 parts of Pollen, 10 parts of Ophiopogon japonicus, 22 parts of Pharbitis chinensis, 24 parts of Mulberry Leaf, 20 parts of Tripterygium wilfordii

[0094] Preparation of drug extract: Weigh each raw material according to the above weight parts, wash it, add 3 times purified water to soak for 30 minutes, then decoct for 40 minutes, filter, decoct it 3 times, combine the filtrate, concentrate the filtrate to a drug content of 1g / mL (0.1g of crude drug per milliliter), sterilize, refrigerate and set aside. When using, dilute each drug extract with liver infusion medium to a drug concentration of 50mg / mL.

[0095] 2 Test methods

[0096] 2.1 Pigeon-derived Trichomonas strains

[0097] The bacteria were isolated from the oral cavity and sinus of sick young pigeons in a breeding farm in Henan Province and stored in liquid nitrogen for 3 months. After resuscitation, they were inoculated with liver infusion medium and cultured aseptically at 37°C. The bacteria were passaged once every 24 hours and tested after stabilization for 1 week.

[0098] 2.3 Determination of the anti-Trichomonas pigeon activity in vitro of different Chinese medicine composition extracts

[0099] The pigeon Trichomonas that had been cultured stably for three generations in vitro was centrifuged to remove dead and weak parasites, and the liver extract was used to prepare a density of 1×10 6 250 μL of live worm suspension was mixed with 250 μL of different Chinese medicine composition extracts and added to 1.5 mL centrifuge tubes, which were experimental groups 1-5. At the same time, culture medium was used instead of drugs as a blank control group, and each group was repeated 3 times. Each group of pigeon trichomonas was cultured in a 37°C incubator, and samples were taken at 2, 4, 8, 12, and 24 hours, respectively, and the pigeon trichomonas mortality rate was calculated using a hemocytometer.

[0100] Criteria for judging whether an insect is dead or alive: a living insect moves, and its flagella or undulating membrane keeps undulating; a dead insect becomes round, its structure becomes blurred, and its body becomes granular; the insect, flagella and undulating membrane stop moving or even rupture.

[0101] 3 Results of in vitro anti-Trichomonas pigeon activity of different Chinese medicine composition extracts

[0102] The results are shown in Table 1. Within the same drug time, the extracts of drugs 1-5 all showed a certain killing effect on pigeon trichomonas. After 8 hours of drug action, the mortality rate of pigeon trichomonas in experimental group 1 was 97.69%, which was much higher than that in experimental groups 2-4, with a very significant difference (P<0.05); after 12 hours of drug action, only the mortality rate of pigeon trichomonas in experimental group 1 reached 100%; after 24 hours of drug administration, the mortality rates of pigeon trichomonas in experimental groups 4 and 5 reached 100%, and at the end of the experiment in experimental groups 2 and 3, the mortality rates of pigeon trichomonas in the two groups did not reach 100%.

[0103] The above results indicate that the Chinese medicine composition in the fermented Chinese medicine preparation of the present invention has a reasonable compatibility, and the components synergize with each other to achieve a good in vitro inhibitory effect on pigeon trichomonas. The lack of any component will significantly affect its activity against pigeon trichomonas.

[0104] Table 1 Trichomonas mortality rate of pigeons in each group at different medication time (%)

[0105]

[0106]

[0107] Note: In the same period, if the shoulder letters are the same or no letter, P>0.05; if the shoulder letters are different, P<0.05.

[0108] Test Example 2

[0109] Verification of the therapeutic effect of the fermented Chinese medicine preparation of the present invention on pigeon trichomoniasis

[0110] 1 Materials and methods

[0111] 1.1 Experimental animals

[0112] Sixty 21-day-old young pigeons infected with Trichomonas were selected from a breeding farm, and 15 21-day-old young pigeons not infected with Trichomonas were selected from the same breeding farm.

[0113] 1.2 Experimental drugs

[0114] The fermented Chinese medicine preparation of the present invention is prepared according to the method described in Example 11;

[0115] The Chinese medicine composition of the present invention comprises the following raw materials: 22 parts of Senecio radix, 26 parts of Juncus effusus, 20 parts of Gentiana scabra, 16 parts of Nasturtium, 12 parts of Gardenia jasminoides, 8 parts of Pollen Typhae, 10 parts of Ophiopogonis, 22 parts of Pharbitidis seeds, 24 parts of Morus alba, 20 parts of Tripterygium wilfordii, 12 parts of Notopterygium wilfordii, 10 parts of Atractylodes lancea, and 10 parts of Patchouli grandiflora, washed, dried, and ultrafinely ground to obtain Chinese medicine powder;

[0116] Metronidazole (Metronidazole): Prepare a 0.05% aqueous solution with purified water

[0117] 1.3 Grouping and processing

[0118] The young pigeons infected with Trichomonas were randomly divided into 4 groups, each with 15 pigeons. The experiment adopted a single-factor completely randomized experimental design, and the fermented Chinese medicine preparation group was gavaged with 0.5 g / (time / pigeon) of the fermented Chinese medicine preparation of the present invention; the Chinese medicine control group was gavaged with 0.5 g / (time / pigeon) of Chinese medicine powder; the Western medicine control group was gavaged with 2 mL / (time / pigeon) of metronidazole solution; the positive control group was gavaged with an equal dose of normal saline; each group was given the drug twice a day for 7 consecutive days.

[0119] Fifteen young pigeons not infected with Trichomonas were used as a preventive group and intragastrically administered with 0.5 g / (time / pigeon) of the fermented Chinese medicinal preparation of the present invention, twice a day, for 7 consecutive days.

[0120] The feeding conditions and immunization procedures were consistent among the groups.

[0121] 1.4 Detection indicators and measurement methods

[0122] 1.4.1 Growth index determination

[0123] The body weight of each group of pigeons was measured before and after administration, and the mortality rate of each group of pigeons during the experimental period was observed and counted.

[0124] 1.4.2 Trichomonas detection

[0125] On the 0th, 1st, 3rd and 7th days of the experiment, crop secretions of pigeons were collected and placed in 1.5-mL centrifuge tubes containing 0.5 mL of normal saline. The tubes were sealed and brought back to the laboratory. Live Trichomonas were counted using a hemocytometer within 4 hours after sampling.

[0126] 1.4.3 Determination of blood biochemical indicators

[0127] After the administration, blood was collected from the right vein of the pigeons in each group, and the levels of AST, ALT, IL-6, and IL-1β in serum were detected using enzyme-linked immunosorbent assay kits.

[0128] 1.5 Data Analysis

[0129] Statistical analysis was performed using Prism 8.0.2, and one-way analysis of variance was used for comparison among groups. The results are expressed as mean ± standard deviation The data were analyzed using t-test. P < 0.05 was considered statistically significant.

[0130] 2 Results

[0131] 2.1 Effect of the fermented Chinese medicine preparation of the present invention on the body weight of pigeons with trichomoniasis

[0132] The results are as follows Figure 1 As shown, before medication, the weight of sick pigeons in each disease group decreased significantly compared with the prevention group, and the difference was extremely significant (P<0.001); the weight of sick pigeons in the positive control group continued to decrease during the experiment; compared with before medication, the weight of sick pigeons in each medication group increased to varying degrees, and the weight of the fermented Chinese medicine preparation group and the Chinese medicine control group after medication was significantly higher than the weight before medication (P<0.001 or P<0.05), and the weight of sick pigeons in the fermented Chinese medicine preparation group increased more. The results show that after the Chinese medicine powder is fermented by probiotics, it is easier to absorb in the animal body. At the same time, probiotics can promote the body's intestinal health and are more conducive to restoring the weight of sick pigeons.

[0133] During the experiment, 7 deaths of sick pigeons occurred in the positive control group, no deaths of sick pigeons occurred in the fermented Chinese medicine preparation group and the Chinese medicine control group, 2 deaths of sick pigeons occurred in the western medicine control group, no deaths of sick pigeons occurred in the prevention group, and no confirmed sick pigeons occurred in the prevention group.

[0134] 2.2 Anti-trichomonas activity of the fermented Chinese medicine preparation of the present invention in vivo

[0135] like Figure 2 As shown, in the positive control group, as time went on, the number of Trichomonas in the sick pigeons increased in a step-by-step manner, with significant differences (P<0.001); in each drug-treated diseased group, the number of Trichomonas in each group was significantly reduced 1d after drug administration (P<0.001), and continued to decrease as the treatment time increased; during the experimental period, the number of Trichomonas in the preventive group did not change significantly, indicating that the fermented Chinese medicine preparation of the present invention has a good preventive effect on Trichomonas.

[0136] like Figure 3 As shown, on the 1st, 3rd and 7th days of administration, the number of Trichomonas in the sick pigeons in the fermented Chinese medicine preparation group was significantly lower than that in the Chinese medicine control group and the Western medicine control group (P<0.05), and there was no significant difference between the Chinese medicine control group and the Western medicine control group (P>0.05). This result shows that the killing effect of the Chinese medicine composition provided by the present invention on Trichomonas is equivalent to that of metronidazole, and the Chinese medicine composition can significantly improve its killing effect on Trichomonas after probiotic fermentation.

[0137] 2.3 Effect of the fermented Chinese medicine preparation of the present invention on serum biochemical indices of trichomoniasis

[0138] like Figure 4As shown, after the administration, compared with the positive control group, each sick pigeon administration group can significantly reduce the content of inflammatory factors IL-6 and IL-1β in the serum of sick pigeons, with significant differences (P<0.0001); among them, the content of inflammatory factors IL-6 and IL-1β in the serum of sick pigeons in the fermented Chinese medicine preparation group was significantly lower than that in the Chinese medicine control group and the Western medicine control group, with significant differences (P<0.05). This result shows that the fermented Chinese medicine preparation provided by the present invention has excellent anti-inflammatory and anti-inflammatory effects.

[0139] Severe Trichomonas infection in pigeons can cause hepatosplenomegaly and the appearance of gray-white nodules with clear boundaries on the surface, so changes in liver-related indicators are of great significance for the diagnosis of Trichomonas infection. Figure 5 As shown, after the administration, the ALT and AST levels in the fermented Chinese medicine preparation group, the Chinese medicine control group and the Western medicine control group were significantly lower than those in the positive control group (P<0.01), and there was no significant difference compared with the prevention group (P>0.05). This result shows that the fermented Chinese medicine preparation of the present invention has a repair and preventive effect on liver damage caused by Trichomonas infection.

[0140] 3 Conclusion

[0141] The above results show that the fermented Chinese medicine preparation of the present invention can significantly reduce the number of Trichomonas in sick young pigeons, has a significant killing effect on Trichomonas, avoids the death of sick young pigeons, and restores the weight of sick pigeons; at the same time, the fermented Chinese medicine preparation of the present invention has excellent anti-inflammatory and anti-inflammatory effects, and has a repair and preventive effect on liver damage caused by Trichomonas infection. The fermented Chinese medicine preparation of the present invention also has a good preventive effect on Trichomonas, can significantly reduce the infection rate of Trichomonas in pigeons, and provides a new drug for the treatment and prevention of Trichomonas in birds.

[0142] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A fermented Chinese medicine preparation for treating avian Trichomonas, characterized in that: The preparation is made of a traditional Chinese medicine composition and composite probiotics. The traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 10-30 parts of Senecio radix, 10-30 parts of Juncus effusus, 10-30 parts of Gentiana scabra, 5-20 parts of Nasturtium, 5-20 parts of Gardenia jasminoides, 5-20 parts of Pollen Typhae, 5-20 parts of Ophiopogonis aconitifolia, 10-30 parts of Pharbitidis seeds, 10-30 parts of mulberry leaves, 10-30 parts of Tripterygium wilfordii, 5-20 parts of Notopterygium wilfordii, 5-20 parts of Atractylodes macrocephala, and 5-20 parts of Patchouli grandiflora.

2. A fermented Chinese medicine preparation for treating avian Trichomonas as claimed in claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 15-25 parts of Senecio herb, 20-30 parts of Juncus effusus, 15-25 parts of Gentiana scabra, 10-20 parts of Nasturtium, 5-15 parts of Gardenia jasminoides, 5-15 parts of Pollen Typhae, 5-15 parts of Ophiopogon japonicus, 15-25 parts of Pharbitidis seeds, 20-30 parts of mulberry leaves, 15-25 parts of Tripterygium wilfordii, 5-15 parts of Notopterygium wilfordii, 5-15 parts of Atractylodes macrocephala, and 5-10 parts of Patchouli grandiflora.

3. A fermented Chinese medicine preparation for treating avian Trichomonas according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 22 parts of Senecio radix, 26 parts of Juncus effusus, 20 parts of Gentiana scabra, 16 parts of Nasturtium, 12 parts of Gardenia jasminoides, 8 parts of Pollen Typhae, 10 parts of Ophiopogon japonicus, 22 parts of Pharbitidus seeds, 24 parts of Morus alba, 20 parts of Tripterygium wilfordii, 12 parts of Notopterygium wilfordii, 10 parts of Atractylodes macrocephala, and 10 parts of Patchouli grandiflora.

4. A fermented Chinese medicine preparation for treating avian Trichomonas according to claim 1, characterized in that: The composite probiotics are composed of Lactobacillus plantarum, Enterococcus faecalis, and Saccharomyces cerevisiae in the following weight ratio of 1-2:0.5-1:0.5-1; the number of live bacteria of each probiotic is: Lactobacillus plantarum ≥ 1.5×10 10 CFU / g, Enterococcus faecalis ≥9×10 9 CFU / g, brewer's yeast ≥9×10 8 CFU / g.

5. A fermented Chinese medicine preparation for treating avian Trichomonas according to claim 1, characterized in that: The composite probiotics are composed of plant lactobacillus, enterococcus faecalis and brewer's yeast in the following weight ratio of 1-1.6:0.5-0.8:0.5-0.

8.

6. A fermented Chinese medicine preparation for treating avian Trichomonas according to claim 1, characterized in that: The composite probiotics are composed of Lactobacillus plantarum, Enterococcus faecalis and Saccharomyces cerevisiae in the following weight ratio of 1.4:0.7:0.

5.

7. The method for preparing a fermented Chinese medicinal preparation for treating avian Trichomonas according to any one of claims 1 to 6, characterized in that: The fermented Chinese medicine preparation is prepared according to the following steps: (1) Weighing Senecio radix, Juncus wickerensis, Gentiana scabra, Nasturtium, Gardenia jasminoides, Pollen Typhae, Ophiopogon japonicus, Pharbitis chinensis, Morus alba, Tripterygium wilfordii, Notopterygium wilfordii, Atractylodes lancea, and Patchouli chinensis according to the above weight proportions, washing, drying, crushing, and passing through a 100-mesh sieve to obtain a fermentation raw material for later use; (2) weighing Lactobacillus plantarum, Enterococcus faecalis, and Saccharomyces cerevisiae according to the above weight portions to obtain a composite probiotic powder, and then mixing 10-20% of the composite probiotic powder, 5-10% of glucose, 5-10% of cellulase, and 60-80% of purified water, stirring well, and culturing in a closed manner at 37° C. for 24 hours to obtain an activated bacterial solution for standby use; (3) The fermentation raw material obtained in step (1) is uniformly mixed with 0.2-0.5 times of purified water, and then uniformly mixed with the activated bacterial solution obtained in step (2), and then placed in a sealed container for anaerobic sealed fermentation at a fermentation temperature of 30-40° C., a fermentation humidity of 30-40%, and a fermentation time of 3-5 days to obtain the fermented Chinese medicine preparation.

8. Use of the fermented Chinese medicinal preparation according to any one of claims 1 to 6 in the preparation of a medicament for treating avian trichomoniasis, characterized in that: The fermented Chinese medicine preparation can be prepared into any oral dosage form acceptable to animals according to conventional methods, and the dosage form includes oral liquid, freeze-dried powder, granules, tablets, and pills.

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