Ginseng-containing collateral-dredging health-care wine as well as preparation method and application thereof

Abstract

The invention relates to the technical field of health-care wine preparation, and provides ginseng-containing collateral-dredging health-care wine as well as a preparation method and application thereof. The collateral-dredging health-care wine takes white spirit as base wine, and is prepared from traditional Chinese medicinal material extract liquid, and the traditional Chinese medicinal materials comprise ginseng, peach kernel, hawthorn, acanthopanax sessiliflorus, radix puerariae, seville orange flower and pawpaw. The health-care wine provided by the invention is reasonable in vinosity component distribution and unique in flavor and taste, has the effects of expelling wind, removing cold, soothing channels, tonifying qi, promoting blood circulation and dredging collaterals, and can meet the requirements of people on delicious taste and health.

Description

Technical Field

[0001] The invention relates to the technical field of health wine preparation, in particular to a ginseng-containing meridian-opening health wine and a preparation method and application thereof. Background Art

[0002] The history of medicinal wine can be traced back to the Shang Dynasty 3,000 years ago. The oracle bone inscriptions of the Shang Dynasty recorded that "Changqi wine" was brewed with the fragrance of hundreds of herbs and turmeric. "The effect of wine mash on the stomach and intestines is similar to that of wine mash," recorded the view of the "miracle doctor" Bian Que that wine mash could be used to treat gastrointestinal diseases.

[0003] The oldest record of Chinese medicinal wine recipes and production methods is in the "52 Disease Prescriptions". The "Health Prescriptions" and "Miscellaneous Treatment Prescriptions" record a total of 35 medicinal wine recipes for treating carbuncle, snake bites, scabies and other diseases, including internal and external medicinal wines. In the "Health Prescriptions", there are a total of six identifiable medicinal wine recipes, among which the second recipe of "Lao Li Zhong" includes the entire medicinal wine production process, the method of taking, the main functions and indications, etc., creating a precedent for the combination of wine and medicine to treat diseases.

[0004] Especially in the past ten years, with the joint efforts of traditional Chinese medicine practitioners and medicinal liquor research and production personnel, a comprehensive summary and conclusion has been made on the development history of Chinese medicinal liquor, the characteristics and applications of Chinese medicinal liquor, the process and quality, etc., which are not only reliable in quality and significant in efficacy, but also deeply trusted and welcomed by patients and the general public.

[0005] In modern society, the pace of life of urban people is accelerating, and they lack necessary physical exercise. Many people are in a sub-healthy state, and even have meridian blockage. The Yellow Emperor's Classic of Internal Medicine points out that meridians are channels that run the body's qi, connect the internal organs and the body surface and all parts of the body. They are the regulatory system of human functions, just like the water pipelines in the city, which transport the body's true qi to all parts of the body. From the perspective of traditional Chinese medicine, if the meridians are blocked, it will cause a person's physical condition to decline and even cause disease problems. If the meridians are blocked, the body will feel pain, the physique will be cold, and some parts of the body will feel sore. At this time, you can take some medicinal wine that promotes blood circulation and dredges to dredge the meridians and achieve the effect of strengthening the body. Summary of the invention

[0006] The purpose of the present invention is to provide a ginseng-containing collateral-dredging health wine and a preparation method and application thereof. The health wine of the present invention has the effects of dispelling wind, dispersing cold, relaxing meridians, invigorating qi, activating blood circulation and dredging collaterals, can improve various problems caused by meridian obstruction, and has no toxic side effects.

[0007] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0008] A health wine containing ginseng for promoting blood circulation, with sauce-flavor liquor (sorghum, wheat, water) as base liquor, ginseng (artificially cultivated), kudzu root, peach kernel, hawthorn, papaya, short-stemmed acanthopanax, and daidaihua as raw materials, carefully prepared through specific processes such as batching, extraction, filtration, blending, and packaging, and using scientific traditional processes, it has the effects of dispelling wind, dispersing cold, relaxing meridians, invigorating qi, activating blood circulation, and promoting blood circulation. Suitable for people with bone and joint discomfort such as wind-cold dampness, tendon and bone pain; people with chronic diseases / sub-health such as spleen and stomach deficiency, qi and blood deficiency, and cold hands and feet; people with sub-health such as poor sleep quality and easy fatigue; and other people with health needs. With sauce-flavor liquor as base liquor, the wine is spicy, sweet, and warm in nature. It can harmonize blood circulation, dispel cold and strengthen the spirit, and promote the potency of the medicine. Wine can be used as a medicine guide to improve the efficacy of the medicine.

[0009] The ginseng-containing collateral-draining health-care wine provided by the invention is prepared from Chinese medicinal material extracts with white wine as base wine. The Chinese medicinal materials include: 8-30 parts of ginseng, 5-20 parts of peach kernels, 5-20 parts of hawthorns, 1-5 parts of Acanthopanax short-stalked, 1-10 parts of Pueraria root, 5-20 parts of Dai Dai Hua and 1-20 parts of papaya.

[0010] The preferred Chinese medicinal materials for the meridian-dredging health-care wine include: 20 parts of ginseng, 10 parts of peach kernels, 15 parts of hawthorns, 3.3 parts of Acanthopanax short-stalked, 5 parts of Pueraria root, 10 parts of daisy flower and 15 parts of papaya.

[0011] The preferred Chinese medicinal materials for the meridian-dredging health-care wine include: 30 parts of ginseng, 5 parts of peach kernels, 20 parts of hawthorn, 5 parts of Acanthopanax short-stalked, 5 parts of Pueraria root, 20 parts of daisy flower and 5 parts of papaya.

[0012] The preferred Chinese medicinal materials of the meridian-dredging health-care wine include: 10 parts of ginseng, 20 parts of peach kernels, 5 parts of hawthorn, 1 part of Acanthopanax short-stalked, 10 parts of Pueraria root, 5 parts of Daisy flower and 20 parts of papaya.

[0013] The Chinese medicinal materials for the meridian-dredging health wine may also include any one or more of Cordyceps militaris, American ginseng, Gastrodia elata, Polygonatum sibiricum, mulberry, wolfberry, black-necked snake, venison, Hovenia dulcis, Cassia seed or Ziziphus jujuba.

[0014] The weight proportions of the above raw materials are as follows: 1-30 parts of Cordyceps militaris, 5-30 parts of American ginseng, 5-30 parts of Gastrodia elata, 5-30 parts of Polygonatum sibiricum, 5-30 parts of mulberry, 5-30 parts of wolfberry, 5-20 parts of Garcinia serpentis, 5-20 parts of venison, 5-30 parts of Hovenia dulcis, 5-30 parts of Cassia seed, and 5-30 parts of Ziziphus jujuba.

[0015] The preferred Chinese medicinal materials of the Tongluo health wine include: 5-25 parts of Cordyceps militaris:

[0016] The above-mentioned Chinese medicinal material raw materials need to be soaked in white wine to obtain the Chinese medicinal material extract, and the weight ratio of the Chinese medicinal material to the white wine is 1:10-15.

[0017] When preparing the meridian-dredging health-care wine, the amount of the Chinese medicinal material extract added is 3-10% of the base wine.

[0018] In the meridian-dredging health-care wine provided by the invention, the volume percentage of alcohol in the white wine used is 30% to 70%.

[0019] Preferably, the volume percentage of alcohol in the liquor is 50% to 55%.

[0020] The preparation method of the Tongluo health-care wine provided by the present invention comprises the following steps:

[0021] 1) Clean and dry the Chinese medicinal materials;

[0022] 2) Weigh the required Chinese medicinal materials according to the formula;

[0023] 3) adding the weighed Chinese medicinal materials into white wine and soaking and extracting for 14 to 42 days, filtering to obtain the Chinese medicinal materials extract;

[0024] 4) Mixing the base wine with the Chinese medicinal material extract, with the Chinese medicinal material extract adding in a proportion of 3-10%;

[0025] 5) Filter and bottle the prepared liquor.

[0026] Preferably, in step 3), the soaking and extraction temperature is 10-35° C., and the humidity is 75%-85%; in step 4), the mixing temperature is 15-30° C., and the humidity is 75%-85%.

[0027] In the Tongluo health-care wine provided by the invention, the Chinese medicinal materials are Chinese medicinal decoction pieces.

[0028] In the meridian-dredging health-care wine provided by the present invention, the liquor is a Maotai-flavor liquor, preferably a Maotai-flavor liquor brewed by Kunsha process.

[0029] The invention also provides the use of the collateral-dredging health-care wine in preparing the health-care wine which can dispel wind, relieve cold, soothe meridians, invigorate qi, activate blood circulation and dredge collaterals.

[0030] The beneficial effects of the collateral-draining health-care wine of the present invention are as follows:

[0031] 1. Compatibility of Chinese herbal medicines

[0032] Ginseng: replenishes qi and dredges meridians. The Shennong's Herbal Classic states that it is a top-grade product that "tonifies the five internal organs, calms the spirit, stabilizes the soul, stops panic, and removes evil spirits...it makes you happy and intelligent, and long-term use makes you lighter and prolongs your life." The Qing Dynasty's Materia Medica Harms and Benefits states that it "tonifies all internal organs with qi deficiency, promotes fluid production and relieves restlessness, improves hearing and vision, calms the spirit, stops panic, dredges blood vessels, and makes the stomach open when qi is strong, and food is digested when qi is harmonious." Peach kernel: activates blood circulation and dredges meridians. The Song Dynasty's Zhenglei Materia Medica states that it "prolongs life, removes wind, makes the skin smooth," and "maintains youth and prolongs life." The Ming Dynasty's Materia Medica Zhengyao states that it "breaks blood stasis in all meridians." Hawthorn: promotes qi and dissipates blood stasis. The Yuan Dynasty's Riyong Materia Medica states that it "transforms food accumulation, relieves qi stagnation, strengthens the stomach and widens the diaphragm, and eliminates blood stasis and qi lumps." Papaya: relaxes muscles and activates collaterals. The "Thunder God Pao Zhi Lun" of the Northern and Southern Dynasties states that it "regulates the Ying and Wei, and helps the Qi of the grain". Wang Haogu of the Yuan Dynasty said that it "removes dampness and harmonizes the stomach, nourishes the spleen and benefits the lungs". The "Bencao Zaixin" of the Qing Dynasty states that it "reinforces the liver and harmonizes the spleen and stomach, activates blood circulation and dredges the meridians". Pueraria root: dredges the meridians and activates the collaterals. It is recorded in the "Shennong Bencao Jing". Li Gao of the Jin and Yuan Dynasties said that it has a light and floating Qi, encourages the stomach Qi to go up, produces body fluids, and relieves muscle heat. Zhang Shanlei of the Qing Dynasty stated in the "Bencao Zhengyi" that it "can best promote the clear Yang Qi of the spleen and stomach". Daidaihua: regulates Qi and widens the chest, dredges the collaterals, and comes from the "Shennong Bencao Jing". Acanthopanax short-stemmed: removes rheumatism, strengthens the muscles and bones, replenishes the essence and improves intelligence. The "Shennong Bencao Jing" lists it as a top grade.

[0033] Ginseng is used as the main ingredient, which can greatly replenish vital energy, invigorate qi and dredge collaterals, so that qi is vigorous and blood can flow. Peach kernel and hawthorn are used as assistant medicines, which can invigorate blood circulation and dredge collaterals, remove blood stasis and make collaterals unblocked. Acanthopanax short-stalked and papaya are used as assistant medicines, which can dispel rheumatism, strengthen muscles and bones, relax muscles and activate collaterals, and kudzu root is used as assistant medicines, which is spicy and can move, can dredge meridians and activate collaterals. The above-mentioned medicinal flavors are used together to play the effects of invigorating qi and activating blood circulation, dredge meridians and activate collaterals. Daidaihua is used as an assistant medicine, which is sweet, slightly bitter, and flat in nature and flavor. It has the functions of regulating qi and widening the chest, dredging collaterals, and making the medicine replenished without being greasy and moving without being stagnant. All the medicines are used together to invigorate the middle and imply communication, and play the effects of dispelling wind, dispersing cold, relaxing meridians, invigorating qi, activating blood circulation and dredge collaterals.

[0034] 2Formula and process advantages

[0035] The formula with the effects of dispelling wind, relieving cold, soothing the meridians, invigorating qi, activating blood circulation and dredging collaterals is composed of edible and medicinal materials, authentic medicinal materials and new food raw materials: ginseng (artificially cultivated), peach kernel, hawthorn, papaya, Acanthopanax short-stalked, Pueraria root, and Daidaihua are extracted through a traditional extraction process for 14 to 42 days to obtain the extract.

[0036] 4Product Features

[0037] Kunsha brewing technology for authentic Maotai-flavor liquor (12987): Kunsha brewing technology is the best in Maotai liquor, characterized by a long production cycle and long storage time of base liquor; select high-quality sorghum raw materials, after two feedings, nine steamings, eight fermentations, seven wine extractions, one production cycle per year, and storage in pottery jars for four years, the brewed Maotai-flavor liquor has a mellower Maotai flavor, a fuller body, and a richer quality.

[0038] The red tassel sorghum, a special variety of the Chishui River, forms special aromatic compounds and polyphenols after fermentation. It is the best raw material for brewing sauce-flavored liquor and is known as the "soy sauce-flavored meat".

[0039] Winter wheat is used to make the koji, and the sauce wine is fermented in a solid state. It has a prominent aroma and a mellow taste, and is known as the "bone of sauce aroma". The water from the Chishui River is used as the brewing water source. The water is pure, sweet and refreshing, and rich in various trace elements. The wine brewed has a mellow flavor and is known as the "blood of sauce aroma".

[0040] The health wine provided by the present invention is prepared from Chinese herbal medicine extract with Maotai-flavor liquor as base wine, and the wine quality components are reasonably distributed, the acid ester ratio is moderate, and the flavor of the prepared wine is combined with the unique flavor of Chinese medicine, so that the flavor and taste of the prepared wine are unique, and high sensory evaluation scores are obtained in color, appearance, aroma, taste and touch. Compared with the base wine, the sensory scores such as color and aroma, the content of phenolic substances and the free radical scavenging rate of the health wine are significantly improved. The health wine has the effects of improving microcirculation, relieving physical fatigue, cold hands and feet and joint pain, improving sleep quality, etc., and can meet people's needs for deliciousness and health. DETAILED DESCRIPTION

[0041] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0042] Embodiment 1:

[0043] A ginseng-containing meridian-dredging health-care wine is prepared with white wine as a base wine and from Chinese medicinal material extracts. The Chinese medicinal materials include: 20 parts of ginseng, 10 parts of peach kernels, 15 parts of hawthorns, 3.3 parts of Acanthopanax short-stalked, 5 parts of Pueraria root, 10 parts of Daidaihua and 15 parts of papaya.

[0044] The preparation method of the collateral-clearing health-care wine is as follows:

[0045] (1) Medicinal material processing: Ginseng, peach kernel, hawthorn, Acanthopanax breviscapus, Pueraria root, papaya, and daisy flower were sorted, cleaned, and dried at 50°C.

[0046] (2) Weighing: Weigh the required medicinal materials according to the formula and pack them into non-woven extraction bags.

[0047] (3) Extraction: Soak the extracted medicinal materials in white wine for 35 days (temperature 20°C, humidity 78%).

[0048] (4) Filtration: After the soaking and extraction is completed, filter the wine to obtain the extract.

[0049] (5) Blending: Blend the base wine with the filtered extract (temperature 25°C, humidity 80%), with the extract added in a ratio of 6% to the base wine.

[0050] (6) The blended wine is filtered, filled, capped, and packaged, and tested according to standards. After passing the inspection, it is put into storage.

[0051] Embodiment 2:

[0052] A ginseng-containing meridian-dredging health-care wine is prepared with white wine as a base wine and from Chinese medicinal material extracts. The Chinese medicinal materials include: 20 parts of ginseng, 10 parts of peach kernel, 15 parts of hawthorn, 3.3 parts of short-stemmed acanthopanax, 5 parts of kudzu root, 10 parts of daidai hua, 15 parts of papaya and 6.7 parts of cordyceps militaris.

[0053] The preparation method of the collateral-clearing health-care wine is as follows:

[0054] (1) Medicinal material processing: Ginseng, peach kernel, hawthorn, Acanthopanax short-stemmed, Pueraria root, papaya, daisy flower and Cordyceps militaris were sorted, cleaned and dried at 50°C.

[0055] (2) Weighing: Weigh the required medicinal materials according to the formula and pack them into non-woven extraction bags.

[0056] (3) Extraction: Soak the extracted medicinal materials in white wine for 35 days (temperature 20°C, humidity 78%).

[0057] (4) Filtration: After the soaking and extraction is completed, filter the wine to obtain the extract.

[0058] (5) Blending: Blend the base wine with the filtered extract (temperature 25°C, humidity 80%), with the extract added in a ratio of 6% to the base wine.

[0059] (6) The blended wine is filtered, filled, capped, and packaged, and tested according to standards. After passing the inspection, it is put into storage.

[0060] Embodiment 3:

[0061] A ginseng-containing meridian-dredging health-care wine is prepared with white wine as a base wine and a Chinese medicinal material extract. The Chinese medicinal materials include: 30 parts of ginseng, 5 parts of peach kernel, 20 parts of hawthorn, 5 parts of short-stemmed acanthopanax, 3 parts of kudzu root, 20 parts of daidai hua and 5 parts of papaya.

[0062] The preparation method of the collateral-clearing health-care wine is as follows:

[0063] (1) Medicinal material processing: Ginseng, peach kernel, hawthorn, Acanthopanax breviscapus, Pueraria root, papaya, and daisy flower were sorted, cleaned, and dried at 50°C.

[0064] (2) Weighing: Weigh the required medicinal materials according to the formula and pack them into non-woven extraction bags.

[0065] (3) Extraction: Soak the extracted medicinal materials in white wine for 28 days (temperature 30°C, humidity 76%).

[0066] (4) Filtration: After the soaking and extraction is completed, filter the wine to obtain the extract.

[0067] (5) Blending: Blend the base wine with the filtered extract (temperature 25°C, humidity 83%), with the extract added in a ratio of 6% to the base wine.

[0068] (6) The blended wine is filtered, filled, capped, and packaged, and tested according to standards. After passing the inspection, it is put into storage.

[0069] Embodiment 4:

[0070] The invention discloses a ginseng-containing meridian-dredging health-care wine, which is prepared from Chinese medicinal material extracts with white wine as a base wine. The Chinese medicinal materials include: 10 parts of ginseng, 20 parts of peach kernels, 5 parts of hawthorn, 1 part of Acanthopanax short-stalked, 10 parts of Pueraria root, 5 parts of Daidaihua and 20 parts of papaya.

[0071] The preparation method of the collateral-clearing health-care wine is as follows:

[0072] (1) Medicinal material processing: Ginseng, peach kernel, hawthorn, Acanthopanax breviscapus, Pueraria root, papaya, and daisy flower were sorted, cleaned, and dried at 50°C.

[0073] (2) Weighing: Weigh the required medicinal materials according to the formula and pack them into non-woven extraction bags.

[0074] (3) Extraction: Soak the extracted medicinal materials in white wine for 42 days (temperature 17°C, humidity 80%).

[0075] (4) Filtration: After the soaking and extraction is completed, filter the wine to obtain the extract.

[0076] (5) Blending: Blend the base wine with the filtered extract (temperature 18.5°C, humidity 82%), with the extract added in a ratio of 3% of the base wine.

[0077] (6) The blended wine is filtered, filled, capped, and packaged, and tested according to standards. After passing the inspection, it is put into storage.

[0078] Embodiment 5:

[0079] The invention discloses a ginseng-containing meridian-dredging health-care wine, which is prepared from Chinese medicinal material extracts with white wine as a base wine. The Chinese medicinal materials include: 15 parts of ginseng, 15 parts of peach kernels, 10 parts of hawthorns, 5 parts of Acanthopanax short-stalked, 1 part of Pueraria root, 12 parts of Daidaihua and 10 parts of papaya.

[0080] The preparation method of the collateral-clearing health-care wine is as follows:

[0081] (1) Medicinal material processing: Ginseng, peach kernel, hawthorn, Acanthopanax breviscapus, Pueraria root, papaya, and daisy flower were sorted, cleaned, and dried at 50°C.

[0082] (2) Weighing: Weigh the required medicinal materials according to the formula and pack them into non-woven extraction bags.

[0083] (3) Extraction: Soak the extracted medicinal materials in white wine for 35 days (temperature 13°C, humidity 82%).

[0084] (4) Filtration: After the soaking and extraction is completed, filter the wine to obtain the extract.

[0085] (5) Blending: Blend the base wine with the filtered extract (temperature 20°C, humidity 79%), with the extract added in a ratio of 10% of the base wine.

[0086] (6) The blended wine is filtered, filled, capped, and packaged, and tested according to standards. After passing the inspection, it is put into storage.

[0087] Example 6

[0088] A ginseng-containing meridian-dredging health-care wine is prepared with white wine as a base wine and from Chinese medicinal material extracts. The Chinese medicinal materials include: 15 parts of ginseng, 15 parts of peach kernels, 10 parts of hawthorns, 5 parts of short-stemmed acanthopanax, 1 part of kudzu root, 15 parts of daidai hua, 3 parts of papaya and 8 parts of cordyceps militaris.

[0089] The preparation method of the collateral-clearing health-care wine is as follows:

[0090] (1) Medicinal material processing: Ginseng, peach kernel, hawthorn, Acanthopanax short-stemmed, Pueraria root, papaya, daisy flower, and Cordyceps militaris were sorted, cleaned, and dried at 50°C.

[0091] (2) Weighing: Weigh the required medicinal materials according to the formula and pack them into non-woven extraction bags.

[0092] (3) Extraction: Soak the extracted medicinal materials in white wine for 28 days (temperature 15°C, humidity 82%).

[0093] (4) Filtration: After the soaking and extraction is completed, filter the wine to obtain the extract.

[0094] (5) Blending: Blend the base wine and the filtered extract (temperature 16°C, humidity 80%), with the extract added in a ratio of 10% of the base wine.

[0095] (6) The blended wine is filtered, filled, capped, and packaged, and tested according to standards. After passing the inspection, it is put into storage.

[0096] Example 7

[0097] A ginseng-containing meridian-dredging health-care wine is prepared with white wine as a base wine and from Chinese medicinal material extracts. The Chinese medicinal materials include: 10 parts of ginseng, 5 parts of peach kernel, 7.5 parts of hawthorn, 1.65 parts of short-stemmed acanthopanax, 2.5 parts of kudzu root, 5 parts of daidai hua, 7.5 parts of papaya and 20 parts of cordyceps militaris.

[0098] The preparation method of the collateral-clearing health-care wine is as follows:

[0099] (1) Medicinal material processing: Ginseng, peach kernel, hawthorn, Acanthopanax short-stemmed, Pueraria root, papaya, daisy flower, and Cordyceps militaris were sorted, cleaned, and dried at 50°C.

[0100] (2) Weighing: Weigh the required medicinal materials according to the formula and pack them into non-woven extraction bags.

[0101] (3) Extraction: Soak the extracted medicinal materials in white wine for 14 days (temperature 23°C, humidity 78%).

[0102] (4) Filtration: After the soaking and extraction is completed, filter the wine to obtain the extract.

[0103] (5) Blending: Blend the base wine with the filtered extract (temperature 25°C, humidity 75%), with the extract added in a ratio of 6% to the base wine.

[0104] (6) The blended wine is filtered, filled, capped, and packaged, and tested according to standards. After passing the inspection, it is put into storage.

[0105] Comparative Example 1:

[0106] A prepared liquor, the dosage of hawthorn is different from that in Example 1, and the Chinese medicinal materials include: 20 parts of ginseng, 10 parts of peach kernel, 25 parts of hawthorn, 3.3 parts of Acanthopanax short-stalked, 5 parts of Pueraria root, 10 parts of Daisy flower and 15 parts of papaya.

[0107] The preparation method of the blended wine is the same as that of Example 1.

[0108] Comparative Example 2:

[0109] A prepared liquor, the dosage of papaya is different from that in Example 1, and the Chinese medicinal materials include: 20 parts of ginseng, 10 parts of peach kernel, 15 parts of hawthorn, 3.3 parts of Acanthopanax short-stalked, 5 parts of Pueraria root, 10 parts of Daisy flower and 25 parts of papaya.

[0110] The preparation method of the blended wine is the same as that of Example 1.

[0111] Comparative Example 3:

[0112] A prepared liquor, compared with Example 1, has different amounts of peach kernel and kudzu root, and Chinese medicinal materials include: 20 parts of ginseng, 3.3 parts of peach kernel, 15 parts of hawthorn, 3.3 parts of Acanthopanax short-stalked, 15 parts of kudzu root, 10 parts of daisy flower and 15 parts of papaya.

[0113] The preparation method of the blended wine is the same as that of Example 1.

[0114] Comparative Example 4:

[0115] A prepared liquor, compared with Example 1, ginseng is replaced by yam, and Chinese medicinal materials include: 20 parts of yam, 10 parts of peach kernel, 15 parts of hawthorn, 3.3 parts of Acanthopanax short-stalked, 5 parts of Pueraria root, 10 parts of Daisy flower and 15 parts of papaya.

[0116] The preparation method of the blended wine is the same as that of Example 1.

[0117] Comparative Example 5:

[0118] A prepared liquor, compared with Example 1, hawthorn is replaced by black plum, and Chinese medicinal materials include: 20 parts of ginseng, 10 parts of peach kernel, 15 parts of black plum, 3.3 parts of Acanthopanax short-stalked, 5 parts of Pueraria root, 10 parts of Dai Dai Hua and 15 parts of papaya.

[0119] The preparation method of the blended wine is the same as that of Example 1.

[0120] Comparative Example 6:

[0121] A prepared liquor, compared with Example 1, papaya is replaced by sea buckthorn, and Chinese medicinal materials include: 20 parts of ginseng, 10 parts of peach kernel, 15 parts of hawthorn, 3.3 parts of Acanthopanax short-stalked, 5 parts of Pueraria root, 10 parts of Daisy flower and 15 parts of sea buckthorn.

[0122] The preparation method of the blended wine is the same as that of Example 1.

[0123] Comparative Example 7:

[0124] A prepared liquor, compared with Example 2, Cordyceps militaris is replaced by Cordyceps militaris fruiting bodies, and Chinese medicinal materials include: 20 parts of ginseng, 10 parts of peach kernels, 15 parts of hawthorn, 3.3 parts of Acanthopanax short-stalked, 5 parts of Pueraria root, 10 parts of Daisy lily, 15 parts of papaya and 6.7 parts of Cordyceps militaris fruiting bodies.

[0125] The preparation method of the blended wine is the same as that of Example 2.

[0126] In order to verify the characteristics of the health wine provided by the embodiments of the present invention, the health wines obtained in Examples 1, 2, 5, and 7 and the prepared wines and base wines obtained in Comparative Examples 1 to 7 were tested for alcohol content, total acid, total ester, DPPH clearance rate, and sensory evaluation. The results are shown in the following table.

[0127] Table 1

[0128]

[0129]

[0130] Among them, sensory evaluation indicators include color (slightly yellow to brownish yellow), appearance (clear and transparent, without obvious precipitation and suspended matter; turn the wine glass, tilt it 45 degrees, and the wine hangs on the glass), aroma (the aroma of sauce wine and Chinese medicine is prominent, coordinated and harmonious; elegant and pure; the aroma of the empty glass lasts for a long time), taste (full and mellow, unique flavor; rich layers, smooth and coordinated; long aftertaste) and touch (put your fingers into the bottom of the glass, dip them in the remaining wine, rub them gently, and feel the viscosity). Each indicator is 20 points, and the full score is 100 points. The specific sensory evaluation score table is shown in Tables 2 to 13.

[0131] Table 2 Example 1 sensory evaluation score table

[0132]

[0133]

[0134] Table 3 Example 2 sensory evaluation score table

[0135]

[0136] Table 4 Example 5 sensory evaluation score table

[0137]

[0138]

[0139] Table 5 Sensory Evaluation Score Table of Example 7

[0140]

[0141]

[0142] Table 6 Sensory evaluation score table of comparative example 1

[0143]

[0144] Table 7 Sensory evaluation score table of comparative example 2

[0145]

[0146]

[0147] Table 8 Sensory evaluation score table of comparative example 3

[0148]

[0149]

[0150] Table 9 Sensory evaluation score table of comparative example 4

[0151]

[0152] Table 10 Sensory evaluation score table of comparative example 5

[0153]

[0154] Table 11 Sensory evaluation score table of comparative example 6

[0155]

[0156]

[0157] Table 12 Sensory evaluation score table of comparative example 7

[0158]

[0159]

[0160] Table 13 Base wine sensory evaluation score table

[0161]

[0162] As can be seen from the data in the table, the total acid and total ester content of the health wine provided by the present invention is relatively high, the content of phenolic substances increases (phenolic substance content 88.57ug / ml in embodiment 1, phenolic substance content 89.13ug / ml in embodiment 2, phenolic substance content 90.12ug / ml in embodiment 5, phenolic substance content 88.31ug / ml in embodiment 7, and phenolic substance content 1.00ug / ml in base wine), the wine quality component distribution is reasonable, the acid ester ratio is moderate, and it has excellent DPPH scavenging ability, and a very high sensory evaluation score is obtained. Compared with the comparative example and the base wine, the health wine of the present invention has obtained a very high sensory evaluation score in terms of color, appearance, aroma, flavor and touch, while comparative examples 1 to 6 have relatively low scores in terms of color, aroma, flavor, and touch, and comparative example 7 has relatively low scores in terms of flavor and touch, and base wine has a low score in terms of color and aroma. The health wine of the present invention makes the mouthfeel of the prepared wine unique by regulating a specific acid ester ratio and combining the unique flavor of traditional Chinese medicine. Compared with the base wine, the sensory scores of color, aroma, phenolic content and free radical scavenging rate of health wine are significantly improved.

[0163] At the same time, the present invention compares the effects of heating extraction of Chinese medicinal materials and immersion extraction of Chinese medicinal materials in Example 1 of the present invention on the total saponins, total flavonoids, DPPH clearance rate of the obtained extract (extract) and the alcohol content, total acid, total ester, DPPH clearance rate and sensory score of the corresponding prepared liquor. The results are shown in Table 14.

[0164] Table 14

[0165]

[0166] It can be seen from the data in the table that compared with the heating reflux extraction, the present invention adopts room temperature immersion extraction, and by adjusting the extraction parameters, the total saponin and total flavonoid contents of the obtained extract are higher, and the acid value ratio of the obtained prepared wine is more moderate, thereby obtaining better DPPH clearance capacity and sensory score.

[0167] Among them, the sensory evaluation score table of the prepared liquor corresponding to the heating (reflux) extraction is shown in Table 15.

[0168] Table 15

[0169]

[0170]

[0171] In addition, referring to the types and amounts of Chinese medicinal materials in Example 1, the total saponins, total flavonoids, DPPH clearance rates of the Chinese herbal medicine pieces and the extracts obtained after the pieces were crushed (10 mesh, 20 mesh, 30 mesh) and the alcohol content, total acid, total ester, DPPH clearance rate and sensory scores of the corresponding health wines were compared. The results are shown in Table 16.

[0172] Table 16

[0173]

[0174]

[0175] It can be seen from the data in the table that compared with the crushing and soaking extraction of medicinal materials, the present invention uses Chinese herbal medicine slices for soaking extraction, and the total flavonoids content of the obtained extract is higher, the DPPH scavenging rate is stronger, and the acid value ratio of the obtained health wine is more moderate, and the DPPH scavenging rate and sensory score are also better than the crushing and soaking extraction. It shows that the health wine obtained by the present invention using Chinese herbal medicine slices as raw materials through a specific soaking extraction process has better taste and flavor and antioxidant effects.

[0176] Among them, the sensory evaluation score tables of the prepared liquors corresponding to the extraction of medicinal pieces, 10-mesh crushing and extraction, 20-mesh crushing and extraction, and 30-mesh crushing and extraction are shown in Tables 17 to 20.

[0177] Table 17

[0178]

[0179]

[0180] Table 18

[0181]

[0182] Table 19

[0183]

[0184]

[0185] Table 20

[0186]

[0187]

[0188] In order to further verify the efficacy of the health wine provided by the present invention, the following efficacy evaluation test was carried out on the health wines obtained in Examples 1 and 2 and the prepared wines and base wines obtained in Comparative Examples 5 and 7.

[0189] Test Example 1

[0190] Effects of sodium iodoacetate on osteoarthritis of the knee joint in rats

[0191] Purpose of the test

[0192] Sodium iodoacetate was injected into the knee joint cavity to induce knee osteoarthritis in rats, and the improvement effects of the health wines (prepared wines) of Examples 1 and 2 and the prepared wines of Comparative Examples 5 and 7 on osteoarthritis pain were evaluated.

[0193] 1 Experimental Materials

[0194] 1.1 Test sample

[0195] The health wine (prepared wine) obtained in Examples 1 and 2 and the prepared wine (alcohol content 53°) obtained in Comparative Examples 5 and 7 are calculated as 1.67 mL / kg (standard body weight 60 kg) per day based on 100 mL intake per day by adults. The specific preparation method is as follows: the prepared wine product is concentrated to make a 4-fold concentrated solution (ethanol volume fraction 6%), 25 mL of the 4-fold concentrated solution is taken, 25.5 mL of 53° base wine is added, and the volume is adjusted to 100 mL with purified water. Preparation of base wine: 28.3 mL of 53° base wine is taken and the volume is adjusted to 100 mL with purified water. The final alcohol concentration of the test sample is 15 degrees, which is provided by the Health Branch of Hebei Yiling Pharmaceutical Research Institute.

[0196] 1.2 Solvents and main reagents

[0197] 1.2.1 Solvent: Deionized water, prepared by Shijiazhuang Yiling Pharmaceutical Co., Ltd.

[0198] 1.2.2 Main reagents:

[0199] Sodium iodoacetate: Aladdin Company, batch number: C2019144.

[0200] 0.9% Sodium Chloride Injection: Shijiazhuang Siyao Co., Ltd., batch number: 220228320.

[0201] Pentobarbital sodium: Shandong Xiya Chemical Industry Co., Ltd., batch number: W5632.

[0202] 1.3 Experimental system

[0203] 1.3.1 Animal strain: SD rats.

[0204] 1.3.2 Animal grade: SPF grade.

[0205] 1.3.3 Animal sex and number: 60 males.

[0206] 1.3.4 Animal age: Animals were approximately 5-6 weeks old when received.

[0207] 1.3.5 Animal weight: The weight range of the animals to be purchased is 140-160g, and the weight range after feeding and dosing is 201-234g.

[0208] 1.3.6 Animal source: purchased from Beijing Huafukang Biotechnology Co., Ltd., certificate number: 110322230101960275, license number: SCXK(Beijing)2019-0008, receipt date: June 8, 2023.

[0209] 1.3.7 Quarantine process: The animals are fed for 5 days for adaptation, during which time they drink water and eat normally, are in good health, and have no signs of disease or death.

[0210] 1.3.8 Feed: SPF maintenance feed for mice and rats, provided by SPF (Beijing) Biotechnology Co., Ltd., feed production license number: SCXK (Beijing) 2019-0010, batch number: 0225SH03090414D, production date: 2023.03.09, shelf life until: 2023.09.08.

[0211] 1.3.9 Drinking water: Drink clean water prepared by the ROB-B experimental animal drinking water system, fill drinking water bottles for free drinking, and conduct a full test of drinking water once a year.

[0212] 1.3.10 Bedding: Ordinary grade corn cob bedding, provided by Beijing Keao Xieli Feed Co., Ltd., batch number: 23049811, production date: 2023.04.01, shelf life until: 2023.09.30, experimental animal production license number: SCXK(Beijing)2020-0010.

[0213] 1.3.11 Identification: Marked with mouse ear number, Beijing Xinhengao Biotechnology Co., Ltd.

[0214] 2 Test methods

[0215] 2.1 Experimental design basis

[0216] Sodium iodoacetate (MIA) is an inhibitor of glyceraldehyde 3-phosphate dehydrogenase. It causes joint lesions by inhibiting glycolysis, thereby targeting avascular cartilage and causing chondrocyte death. Injection of sodium iodoacetate into the rat knee joint cavity can cause degradation and loss of knee joint cartilage, changes in cartilage matrix, synovitis and other lesions similar to human osteoarthritis. Under normal circumstances, when the body receives harmful stimulation signals (mechanical stimulation, thermal stimulation, chemical stimulation, etc.), the pain conduction pathway will transmit the signal to the central nervous system, causing an instinctive leg retraction reflex to avoid injury. Under pathological conditions, such as inflammation, the pain conduction pathway will be abnormal, resulting in hyperalgesia and allodynia. After tissue damage, not only the injured area has an enhanced response to normal harmless stimulation, but also the adjacent uninjured area has an enhanced response to mechanical stimulation, which is the so-called secondary hyperalgesia. Slight stimulation can induce a leg retraction reflex to protect the body from injury, resulting in a decrease in pain threshold.

[0217] 2.2 Dosage and grouping

[0218] 56 quarantine qualified animals were randomly divided into 7 groups according to body weight: sham operation group, model control group, base wine group, Example 1, Example 2, Comparative Example 5, Comparative Example 7, 8 animals in each group. The prepared wine is recommended to be taken by adults every day at 100 mL, which is converted to 1.67 mL / kg per day (standard body weight 60 kg), and the equivalent dose of rats converted according to body surface area is about 10 ml / kg. The dosage of rats in this experiment is calculated according to 2 times the equivalent intake of humans, i.e. 20 ml / kg.

[0219] 2.3 Administration

[0220] Administration by oral gavage.

[0221] 2.4 Preparation and storage of test samples

[0222] No preparation required, store sealed.

[0223] 2.5 Administration of test products

[0224] Disposable sterile syringes and gavage needles of appropriate specifications were used for gavage administration, with a volume of 20 ml / kg. The sham operation group and the model control group were given the same volume of deionized water. The drug was administered once a day for 21 consecutive days.

[0225] 2.6 Model making

[0226] Rats were anesthetized with sodium pentobarbital and fixed in supine position. The hair of the 1 cm area around the left hind knee joint was shaved, disinfected with iodine and deiodinated with 75% alcohol. The knee joint was flexed 45°, and the needle was inserted into the direction of the intercondylar fossa with the patella and the outer edge of the patellar tendon as the insertion point. The needle tip was withdrawn 2 mm after reaching the femoral condyle. The model control group and each drug-treated group were injected with 50 μL of sodium iodoacetate solution (10 mg / ml), and the sham-operated group was injected with 50 μL of normal saline in the left knee joint cavity. Drug administration began on the next day of modeling.

[0227] 2.7 Index detection

[0228] 2.7.1 Pain index: reaction time of rats' soles to mechanical stimulation. On the day after the last administration, metal needles were used to stimulate the soles of the feet, and the time it took the rats to lift their feet was recorded. The time it took the rats to lift their feet was taken as the mechanical threshold, and the percentage of pain threshold increase was calculated.

[0229] 2.7.2 Swelling index: knee joint circumference on the modeling side. After the pain threshold test, the knee joint of the rat on the modeling side was removed, and the surrounding muscles were removed to measure the knee joint circumference.

[0230] 2.8 Notification to relevant staff

[0231] Notify the animal room when purchasing animals, and notify the pathology room for treatment when animals show abnormal conditions.

[0232] 2.9 Instrument system

[0233] Mettler ME104E analytical balance (YL-LE-B008), METTLER TOLEDO.

[0234] DT2000 electronic balance (YL-LE-B002, B043), Changshu Shuangjie Testing Instrument Factory.

[0235] Plantar tester (YL-LE-A083), ugo company.

[0236] 2.10 Statistical methods

[0237] The experimental data are expressed as mean ± standard deviation Representation. SPSS software was used for statistical analysis. One-way ANOVA was used for mean comparison. The homogeneity of variance test was first performed. If the variance was homogeneous, the least significant difference (LSD) method was used. If the variance was unequal, Dunnett's T3 test was used. The independent sample t test was used for comparison between the two groups. The test level was 0.05 and 0.01.

[0238] 3 Results

[0239] 3.1 Mechanical pain threshold

[0240] The test results are shown in Table 21. Compared with the sham operation group: the mechanical threshold of the model group was significantly reduced (P < 0.01), which was manifested as increased mechanical sensitivity.

[0241] Compared with the model group: the pain threshold of the base wine group showed an upward trend, but no statistical difference was found (P>0.05); the mechanical pain threshold of the four Luoshen wine formula groups was significantly increased (P<0.05 or, P<0.01).

[0242] Comparison among the administration groups: the pain threshold of Example 1 group was significantly higher than that of the base wine group (P < 0.01); the pain threshold of Example 2 group was significantly higher than that of the base wine group, Comparative Example 5, and Comparative Example 7 group (P < 0.05 or, P < 0.01); no statistical difference was found between the other groups (P > 0.05). The percentage of pain threshold increase in each administration group is ranked from high to low as follows: Example 2, Example 1, Comparative Example 7, Comparative Example 5, base wine.

[0243] Table 21 Mechanical pain threshold and knee joint circumference after drug administration in each group of the experiment

[0244]

[0245] Note: Compared with the model group: *P<0.05, **P<0.01; compared with Example 1: △△P<0.01; compared with Example 2: #P<0.05, ##P<0.01

[0246] 3.2 Knee circumference

[0247] The test results are shown in Table 21. Compared with the sham operation group, the knee joint circumference of the model control group increased significantly (P<0.01).

[0248] Compared with the model control group, the knee joint circumference of the base wine group showed a decreasing trend, but no statistical difference was found (P>0.05); the knee joint circumference of the four Luoshen wine formula groups was significantly reduced (P<0.05 or, P<0.01).

[0249] Comparison among the drug-administered groups: Only the knee joint circumference of the Example 2 group was significantly smaller than that of the base wine group (P < 0.05), and no statistical difference was found among the other groups (P > 0.05).

[0250] 4 Conclusion

[0251] In this experiment, sodium iodoacetate was injected into the knee joint cavity to induce knee osteoarthritis in rats, and the improvement effect of the prepared liquors obtained in Examples 1 and 2 and Comparative Examples 5 and 7 on osteoarthritis pain was evaluated. The test results showed that the Example group and the Comparative Example group could relieve the joint swelling and pain of the model rats to a certain extent after continuous intragastric administration for 21 days, and the improvement effect was particularly significant in the Example 1 and 2 groups, and the ranking from high to low by the percentage of pain threshold improvement was: Example 2> Example 1> Comparative Example 7> Comparative Example 5> Base liquor.

[0252] Test Example 2

[0253] Study on the anti-fatigue effect on mice

[0254] Purpose of the test

[0255] This test evaluates whether the health wines (formulated wines) of Examples 1 and 2 and the formulated wines of Comparative Examples 5 and 7 have the effect of relieving physical fatigue by observing the weight-bearing swimming time and the rotarod time of mice.

[0256] 1 Experimental Materials

[0257] 1.1 Test Articles

[0258] The health wine (prepared wine) obtained in Examples 1 and 2 and the prepared wine (alcohol content 53°) obtained in Comparative Examples 5 and 7 are calculated as 1.67 mL / kg (standard body weight 60 kg) per day based on 100 mL intake per day by adults. The specific preparation method is as follows: the prepared wine product is concentrated to make a 4-fold concentrated solution (ethanol volume fraction 6%), 41.8 mL of the 4-fold concentrated solution is taken, 26.1 mL of 53° base wine is added, and the volume is adjusted to 100 mL with purified water. Preparation of base wine: 28.3 mL of 53° base wine is taken and the volume is adjusted to 100 mL with purified water. The final alcohol concentration of the test sample is 15 degrees, which is provided by the Health Branch of Hebei Yiling Pharmaceutical Research Institute.

[0259] 1.2 Solvents and main reagents

[0260] 1.2.1 Solvent: Purified water, prepared by Shijiazhuang Yiling Pharmaceutical Co., Ltd.

[0261] 1.3 Experimental system

[0262] 1.3.1 Animal strain: KM mice.

[0263] 1.3.2 Animal grade: SPF grade.

[0264] 1.3.3 Animal sex and number: 75 males, 72 of which were selected for this experiment.

[0265] 1.3.4 Animal age: Animals were approximately 4 weeks old when received.

[0266] 1.3.5 Animal weight: The weight of the animals to be purchased is 16-18g, and the weight range after feeding and dosing is 20-26g.

[0267] 1.3.6 Animal source: purchased from Beijing Huafukang Biotechnology Co., Ltd., certificate number: 110322231103011054, license number: SCXK(Beijing)2019-0008, receipt date: July 13, 2023.

[0268] 1.3.7 Quarantine process: The animals are fed for 5 days for adaptation, during which time they drink water and eat normally, are in good health, and have no signs of disease or death.

[0269] 1.3.8 Feed: SPF maintenance feed for mice and rats, provided by SPF (Beijing) Biotechnology Co., Ltd., feed production license number: SCXK (Beijing) 2019-0010, batch number: 0225SH03090414D, production date: 2023.03.09, shelf life until: 2023.09.08.

[0270] 1.3.9 Drinking water: Drink clean water prepared by the ROB-B experimental animal drinking water system, fill drinking water bottles for free drinking, and conduct a full test of drinking water once a year.

[0271] 1.3.10 Bedding: Ordinary grade corn cob bedding, provided by Beijing Keao Xieli Feed Co., Ltd., batch number: 23049811, production date: 2023.04.01, shelf life until: 2023.09.30, experimental animal production license number: SCXK(Beijing)2020-0010.

[0272] 1.3.11 Identification: Marked with mouse ear number, Beijing Xinhengao Biotechnology Co., Ltd.

[0273] 2 Test methods

[0274] 2.1 Experimental design basis

[0275] The swimming test is a recognized observation indicator for observing the anti-fatigue ability of animals. The rotarod fatigue tester is an ideal instrument for screening and identifying anti-fatigue drugs. Therefore, the length of the mouse's weighted swimming time and rotarod time can reflect the body's endurance.

[0276] 2.2 Dosage and grouping

[0277] 72 quarantine qualified animals were randomly divided into 6 groups according to body weight: blank control group, base wine group, Example 1 group, Example 2 group, Comparative Example 5 group and Comparative Example 7 group, with 12 animals in each group. The recommended daily intake of 100 mL of the prepared wines of the Examples and Comparative Examples for adults is converted to 1.67 mL / kg per day (standard body weight 60 kg), and the equivalent dose of mice converted according to body surface area is about 16.7 ml / kg. The dosage of mice in this experiment is calculated according to 2 times the equivalent intake of humans, i.e. 33.4 ml / kg.

[0278] 2.3 Administration

[0279] Administration by oral gavage.

[0280] 2.4 Preparation and storage of test samples

[0281] No preparation required, store in sealed container and refrigerate.

[0282] 2.5 Administration of test products

[0283] The drug was administered by gavage using a disposable sterile syringe and gavage needle of appropriate specifications, with a volume of 20 ml / kg (0.2 ml / 10 g). The blank control group was given the same volume of purified water. The drug was administered once a day for 30 consecutive days.

[0284] 2.6 Index detection

[0285] 2.6.1 Rotarod test: One hour after administration on the 27th day, the rotarod test was performed. The mouse rotarod fatigue instrument was set to start at 5r / min and finally accelerate to 30r / min, with a total test time of 180s. The mouse was placed on the rotarod, the rotarod was started, and its fall latency was recorded. The mouse practiced once and the formal test was performed the second time.

[0286] 2.6.2 Weighted swimming test: On the second day after the last oral administration, the tail of the mouse was loaded with 5% lead sheet of its body weight and placed in a swimming box (about 50cm×50cm×40cm in size) for swimming. The water depth was not less than 30cm and the water temperature was 25℃±1.5℃. The time from the start of swimming to the death of the mouse was recorded (the mouse head was completely immersed in the water for 8s and could not float to the surface as the end point), which was the weighted swimming time of the mouse. Precautions during swimming: ① Pay attention to the maintenance of water temperature. Too low water temperature will cause muscle spasm and bring errors to the weighted swimming time; ② The number of mice swimming at the same time in each tank should not be too large to avoid collision and choking, which is also inconvenient for researchers to observe; ③ Pay attention to the limbs of the mouse during the whole process to keep it moving. If the mouse is in a floating state with no limbs, a glass rod can be used to stir it nearby to promote its movement.

[0287] 2.7 Notification to relevant staff

[0288] Notify the animal room when purchasing animals, and notify the pathology room for treatment when animals show abnormal conditions.

[0289] 2.8 Instrument system

[0290] Mettler ME104E analytical balance (YL-LE-B008), METTLER TOLEDO.

[0291] DT2000 electronic balance (YL-LE-B002, B043), Changshu Shuangjie Testing Instrument Factory.

[0292] IITC Rotarod Fatigue Apparatus for Rats and Mice (YL-LE-A051), IITC Life Science.

[0293] 2.9 Statistical methods

[0294] The experimental data were expressed as mean ± standard deviation (x ± s). SPSS software was used for statistical analysis, and one-way analysis of variance was used for mean comparison. The homogeneity of variance test was first performed. If the variance was homogeneous, the least significant difference method (LSD) was used. If the variance was unequal, Dunnett's T3 test was used. The independent sample t test was used for comparison between the two groups. The test level was 0.05 and 0.01.

[0295] 3 Results

[0296] 3.1 Rotation time

[0297] As shown in Table 22, compared with the blank control group: although the rod turning time in the base wine group showed an upward trend, there was no statistical difference (P>0.05); the rod turning time in the four formula groups of Luoshen wine was significantly increased (P<0.05 or, P<0.01).

[0298] Comparison among the drug-administered groups: The rod rotation time of group 1 was significantly longer than that of the base wine group (P < 0.05); the rod rotation time of group 2 was significantly longer than that of the base wine group, group 3, and group 4 (P < 0.05 or, P < 0.01). No statistical difference was found among the other groups (P > 0.05).

[0299] Table 22 Rotarod time and weighted swimming time of each group in the experiment

[0300]

[0301] Note: Compared with the blank group: *P<0.05, **P<0.01; compared with formula 1: △P<0.05; compared with formula 2: #P<0.05, ##P<0.01

[0302] 3.2 Swimming time

[0303] As shown in Table 22, compared with the blank control group: although the swimming time of the base wine group showed an upward trend, there was no statistical difference (P>0.05); the swimming time of Example 1, 2 groups and Comparative Example 5, 7 groups were significantly increased (P<0.05 or, P<0.01).

[0304] Comparison among the drug administration groups: The swimming time of Example 1 group was significantly longer than that of the base wine group and Comparative Example 5 group (P < 0.05 or, P < 0.01); The swimming time of Example 2 group was significantly longer than that of the base wine group, Comparative Example 5 and Comparative Example 7 group (P < 0.05 or, P < 0.01). No statistical difference was found among the other groups (P > 0.05).

[0305] 4 Conclusion

[0306] This test evaluates whether the prepared liquors of Examples 1, 2 and Comparative Examples 5, 7 have the function of relieving physical fatigue by observing the length of the rotarod time and weighted swimming time of mice. The test results show that continuous intragastric administration of the Example group and the Comparative Example group for 30 days can significantly improve the rotarod time and weighted swimming time of mice, and has a certain anti-fatigue effect, among which the effect of Example 1 and Example 2 groups is the best.

[0307] Test Example 3

[0308] The nail fold is the skin fold covering the base of the nail and can be used to study the patient's microcirculation.

[0309] This experiment evaluated the improvement effect of the health wine in Example 1 on microcirculation by observing the results of nail fold microcirculation detection.

[0310] The recruited people tasted the health wine obtained in Example 1 and the corresponding base wine respectively, and tasted for 35 days at 50 mL / day*10 days+100 mL / day*25 days. A total of 20 people were recruited, with 10 people in each of the health wine trial group and the base wine trial group. Before the tasting, nail fold microcirculation detection was performed on all subjects. After the tasting, 7 people were randomly selected from each group for nail fold microcirculation detection.

[0311] For nail fold microcirculation detection, the subject sits in front of the detection machine, selects the fourth finger of the non-dominant hand as the inspection finger, drops 1 to 2 drops of cedar oil on the nail fold, and places it under a microscope. Two experienced physicians jointly observe the first row of capillaries under the nail fold dermal papilla and record relevant images. At the same time, weighted integration is performed on the microcirculation morphology, flow pattern and pericyclic condition.

[0312] The blood flow velocity results of the subjects in the health wine trial group are shown in Table 23, and the total microcirculation score results are shown in Table 24.

[0313] Table 23

[0314]

[0315] Table 24

[0316]

[0317]

[0318] The blood flow velocity results of the subjects in the base wine tasting group are shown in Table 25, and the total microcirculation integral results are shown in Table 26.

[0319] Table 25

[0320]

[0321] Table 26

[0322]

[0323] From the above data, it can be seen that the blood flow rate results of the subjects who tasted the health wine showed that the blood flow rate increased by an average of 11.4μm / s before and after the trial, and the difference was significant (P<0.05). After drinking the health wine, the blood flow rate showed a certain trend of improvement; while the blood flow rate results of the subjects who tasted the base wine showed that the blood flow rate increased by an average of only 2.2μm / s before and after the trial. The total microcirculation score results showed that the total microcirculation score of the subjects who tasted the health wine improved by an average of 0.24 points before and after the trial (the lower the total score, the better); while the total microcirculation score of the subjects who tasted the base wine changed by an average of 0.07 before and after the trial. It shows that the health wine provided by the present invention can improve microcirculation to a certain extent, and the effect is significantly better than that of the base wine.

[0324] In addition, the subjects who tasted the health wine of the present invention had problems such as "poor physical strength and easy fatigue", "cold hands and feet", "body and joint pain", and "sleep quality", which were improved to varying degrees after tasting the health wine.

[0325] The health wine provided by the invention can improve microcirculation, relieve physical fatigue, cold hands and feet, and joint pain, help improve sleep quality, and improve the health status of the human body.

[0326] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent substitution or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A health-care wine containing ginseng for promoting blood circulation, It is characterized in that The invention takes liquor as the base liquor and is prepared from Chinese medicinal material extracts. The Chinese medicinal materials include 8-30 parts of ginseng, 5-20 parts of peach kernels, 5-20 parts of hawthorns, 1-5 parts of Acanthopanax short-stalked, 1-10 parts of Pueraria root, 5-20 parts of Dai Dai Hua and 1-20 parts of papaya.

2. The meridian-dredging health wine according to claim 1, It is characterized in that The Chinese medicinal materials include: 20 parts of ginseng, 10 parts of peach kernel, 15 parts of hawthorn, 3.3 parts of Acanthopanax short-stalked, 5 parts of Pueraria root, 10 parts of Daisy flower and 15 parts of papaya.

3. The meridian-dredging health wine according to claim 1, It is characterized in that The Chinese medicinal materials include: 30 parts of ginseng, 5 parts of peach kernel, 20 parts of hawthorn, 5 parts of Acanthopanax short-stalked, 3 parts of Pueraria root, 20 parts of Daisy flower and 5 parts of papaya.

4. The meridian-dredging health wine according to claim 1, It is characterized in that The Chinese medicinal materials include: 10 parts of ginseng, 20 parts of peach kernels, 5 parts of hawthorn, 1 part of Acanthopanax short-stalked, 10 parts of Pueraria root, 5 parts of Daisy flower and 20 parts of papaya.

5. The meridian-dredging health wine according to any one of claims 1 to 4, It is characterized in that The Chinese medicinal materials also include any one or more of Cordyceps militaris, American ginseng, Gastrodia elata, Polygonatum sibiricum, mulberry, wolfberry, black-necked snake, venison, Hovenia dulcis, Cassia seed or Ziziphus jujuba.

6. The meridian-dredging health wine according to any one of claims 1 to 4, It is characterized in that The Chinese medicinal materials are soaked in white wine to obtain a Chinese medicinal material extract, and the weight ratio of the Chinese medicinal materials to the white wine is 1:10-15.

7. The meridian-dredging health wine according to claim 6, It is characterized in that The added amount of the Chinese medicinal material extract is 3-10%.

8. The meridian-dredging health wine according to claim 6, It is characterized in that The volume percentage of alcohol in the liquor is 30% to 70%.

9. The meridian-dredging health wine according to claim 8, It is characterized in that The volume percentage of alcohol in the liquor is 50% to 55%.

10. The meridian-dredging health wine according to any one of claims 1 to 4, It is characterized in that The preparation method of the Tongluo health-care wine comprises the following steps: 1) Clean and dry the Chinese medicinal materials; 2) Weigh the required Chinese medicinal materials according to the formula; 3) adding the weighed Chinese medicinal materials into white wine and soaking and extracting for 14 to 42 days, filtering to obtain the Chinese medicinal materials extract; 4) Mixing the base wine with the Chinese medicinal material extract, with the Chinese medicinal material extract adding in a proportion of 3-10%; 5) Filter and bottle the prepared wine.

11. The meridian-dredging health wine according to claim 10, It is characterized in that In step 3), the soaking and extraction temperature is 10-35° C., and the humidity is 75%-85%; in step 4), the mixing temperature is 15-30° C., and the humidity is 75%-85%.

12. The meridian-dredging health wine according to any one of claims 1 to 4, It is characterized in that The Chinese medicinal materials are Chinese medicinal pieces.

13. The meridian-dredging health wine according to any one of claims 1 to 4, It is characterized in that The liquor is a sauce-flavor liquor.

14. Use of the collateral-dredging health wine according to any one of claims 1 to 4 in the preparation of a collateral-dredging health wine that dispels wind, relieves cold, soothes meridians, replenishes qi, activates blood circulation and dredges the collaterals.

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