Streptomycete and application thereof in prevention and control of fungal diseases of edible fungi
By using Streptocytic carbophila thermophila and its metabolites, the growth of Trichoderma mycelium in fungal diseases of edible fungi has been suppressed, and the problem of difficulty in effectively preventing and treating fungal diseases in the prior art has been solved, and a green and environmentally friendly disease prevention and control effect has been achieved.
Patent Information
- Application Number
- CN202510290794.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-12
- Publication Date
- 2025-06-06
AI Technical Summary
In the prevention and control of fungal diseases of edible fungi, the prior art is difficult to effectively inhibit the pathogenic bacteria of Trichoderma, and it does not affect the normal growth of edible fungal mycelium, which has problems of high technical threshold and high difficulty.
Using Streptomyces thermophilus thermocarboxydus CGMCC No. 32349 and its metabolites, a biological control agent for the prevention and control of fungal diseases of edible fungi was prepared by inhibiting the growth of Trichoderma mycelium.
It significantly inhibits the occurrence of fungal diseases of edible fungi, especially it has a significant inhibitory effect on Trichoderma Corning, Trichoderma Harzite and Fusarium Trichoderma, but has no effect on the growth of edible fungi, solving the environmental pollution and pesticide residue problems of traditional chemical pesticides.
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Figure CN120098852A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of microbial application, and specifically relates to a Streptomyces and an application thereof in the prevention and control of fungal diseases of edible fungi, and more specifically relates to a thermophilic carbon monoxide Streptomyces (Streptomyces thermocarboxydus) and an application thereof in the green prevention and control of fungal diseases of edible fungi. Background Art
[0002] Edible mushrooms refer to mushrooms with large fruiting bodies, fleshy or gelatinous structures, and can be eaten (Ahmad et al., 2013). The edible mushroom industry has become the fifth largest industry in my country's agricultural planting industry after grain, vegetables, fruit trees, and oil crops. In 2023, my country's annual output of edible mushrooms will reach 43.3417 million tons, with an annual output value of 396.557 billion yuan. The edible mushroom industry has the characteristics of recyclability, high protein, fast effect, short cycle, and high output (YU et al., 2020). It is a kind of efficient biological resource with both nutritional, medicinal and ecological values (Cardwell et al., 2018, Zhang et al., 2021, Jesús et al., 2021). The edible mushroom industry has become a dominant industry in some regions. As the world's largest producer of edible mushrooms, China's industry has developed rapidly, but edible mushroom green mold disease has become one of the key factors restricting industrial development. Green mold disease has the characteristics of rapid onset, difficulty in control and high mortality rate. Especially in high temperature and high humidity environment, its spores are spread through the air and are very easy to breed in fungus bags and culture media. At present, the prevention and control of green mold disease is still mainly chemical agents, but the irrational use of traditional chemical pesticides has led to increasingly prominent problems such as environmental pollution and pesticide residues. Biological control meets the development needs of current ecological agriculture. In recent years, with the continuous development of green control technology, the application of biological control in the prevention and control of green mold disease of edible fungi has gradually increased. But the key problem is that both the pathogenic Trichoderma and its host edible fungi are filamentous fungi. In terms of fungicide development and screening, the technical threshold requirements are relatively high. It is necessary to ensure that the pathogenic Trichoderma can be effectively inhibited and that the normal growth of the edible fungus hyphae itself is not affected. Therefore, compared with other agricultural crops, the screening and development of microbial biocontrol products for the prevention and control of fungal diseases of edible fungi is more difficult, the workload is large, and there are technical bottlenecks. Summary of the invention
[0003] One of the purposes of the present invention is to provide a streptomyces.
[0004] The streptomyces provided by the present invention is Streptomyces thermocarboxydus, whose strain number is P8-1 and whose registration number in the General Microbiological Center of China National Microbiological Culture Collection is CGMCC No.32349, hereinafter referred to as Streptomyces thermocarboxydus CGMCC No.32349.
[0005] The nucleotide sequence of the 16S rRNA gene of the thermocarbon monoxide Streptomyces CGMCC No. 32349 contains the DNA molecule shown in SEQ ID NO. 1 in the sequence list.
[0006] The nucleotide sequence of the rpoB gene of the thermocarbon monoxide Streptomyces CGMCC No. 32349 contains the DNA molecule shown in SEQ ID NO. 2 in the sequence list.
[0007] The nucleotide sequence of the 16S rRNA gene of the thermophilic carbon monoxide Streptomyces CGMCC No.32349 was molecularly identified, and a phylogenetic tree was constructed using the NJ method. Figure 5 .
[0008] The nucleotide sequence of the atpD gene of the thermophilic carbon monoxide Streptomyces CGMCC No.32349 was molecularly identified, and a phylogenetic tree was constructed using the NJ method. Figure 6 .
[0009] The nucleotide sequence of the rpoB gene of the thermophilic carbon monoxide Streptomyces CGMCC No.32349 was molecularly identified, and a phylogenetic tree was constructed using the NJ method. Figure 7 .
[0010] The morphological characteristics of the thermocarbon monoxide Streptomyces CGMCC No. 32349 are as follows: after culturing on Gao's No. 1 medium for 7 days, abundant hyphae are produced, the colonies are white, the surface is relatively dry, and lemon yellow soluble pigment is produced; the aerial hyphae are light gray, and chain-like spore chains are produced at the top of the hyphae, and the spores are round or ovoid (see Figure 2 and Figure 4 ).
[0011] Under a 40x optical microscope, the bacterial cells of strain P8-1 have 3-6 loose spirals of spores, and oblong and oval spores. On Gao's synthetic medium No. 1, the base silk is milky white, and the aerial silk is grayish white; the size of the colony is 1-2mm, round in shape, with neat edges, opaque, grayish white in color, convex in the middle, bright and smooth surface, and dry and hard texture.
[0012] The physiological and biochemical characteristics of the thermophilic carbon monoxide streptomyces CGMCC No. 32349 are Gram-positive. On potato culture medium: aerial filaments are gray, basal filaments are fine, colorless or yellow-brown, and the matrix is not stained or brown-yellow.
[0013] Another object of the present invention is to provide a composition, which contains the thermocarbon monoxide Streptomyces CGMCC No.32349.
[0014] The active ingredient of the above composition may be the thermophilic carboxytopentaic bacteria or / and a metabolite of the thermophilic carboxytopentaic bacteria or / and a culture of the thermophilic carboxytopentaic bacteria.
[0015] The above-mentioned culture can be a substance obtained by culturing the thermophilic carbon monoxide streptomyces in a microbial culture medium (i.e., a fermentation product, such as a fermentation broth containing the thermophilic carbon monoxide streptomyces and a substance secreted into a liquid culture medium, or a solid fermentation product containing the thermophilic carbon monoxide streptomyces and a substance secreted into a solid culture medium).
[0016] In the above, the metabolite may be a product obtained by removing the thermophilic carboxytopenta from the culture, such as culturing the thermophilic carboxytopenta in a liquid fermentation medium, collecting the fermentation broth (containing the thermophilic carboxytopenta and substances secreted into the liquid culture medium), removing the thermophilic carboxytopenta from the fermentation broth, and collecting the remaining components of the fermentation broth to obtain the metabolite of the thermophilic carboxytopenta.
[0017] The active ingredients of the above composition may also contain other biological ingredients or non-biological ingredients. Those skilled in the art may determine the other active ingredients of the above composition according to the effects of the composition.
[0018] The above composition can be the culture. The above composition can also be a bacterial agent or a microecological preparation.
[0019] The above-mentioned bacterial agent refers to a live bacterial preparation made by expanding the target microorganisms and using a carrier as an adsorbent to adsorb the fermentation liquid or solid fermentation product of the bacteria.
[0020] The above-mentioned bacterial agents may be in various dosage forms, including but not limited to liquid, emulsion, suspension, powder, granule, wettable powder or water dispersible granule, etc.
[0021] The bacterial agent may further include a carrier as required, and the carrier may be a solid carrier or a liquid carrier.
[0022] The above-mentioned carrier can specifically be at least one of calcium phosphate, rice husk powder, bran, rice bran, skimmed milk powder, maltodextrin, sucrose, glycerol and starch.
[0023] The microecological preparation is a preparation containing microorganisms and / or their metabolites that can be added to the edible fungus cultivation matrix.
[0024] Thermocarboxygenic Streptomyces CGMCC No.32349 or the composition has at least one of the following properties:
[0025] 1) Inhibit pathogens;
[0026] 2) Preparation of pathogen inhibitors;
[0027] 3) Suppress diseases;
[0028] 4) Preparation of disease inhibitors.
[0029] The pathogen is at least one of the following: Trichoderma, Fusarium, more specifically: Trichoderma koningii, Trichoderma harzianum, Fusarium equiseti;
[0030] The disease is a fungal disease of edible fungi, specifically, at least one of a Trichoderma disease and a Fusarium disease of edible fungi;
[0031] The edible fungi include one or more of shiitake mushrooms, oyster mushrooms, black fungus, button mushrooms, morels, and stropharia.
[0032] At least one of the following applications of thermocarbon monoxide Streptomyces CGMCC No.32349 or the composition also belongs to the protection scope of the present invention:
[0033] 1) Inhibit pathogens;
[0034] 2) Preparation of pathogen inhibitors;
[0035] 3) Suppress diseases;
[0036] 4) Preparation of disease inhibitors;
[0037] The pathogen is at least one of the following: Trichoderma, Fusarium, more specifically: Trichoderma koningii, Trichoderma harzianum, Fusarium equiseti;
[0038] The disease is a fungal disease of edible fungi, specifically, at least one of a Trichoderma disease and a Fusarium disease of edible fungi;
[0039] The edible fungi include one or more of shiitake mushrooms, oyster mushrooms, black fungus, button mushrooms, morels, and stropharia.
[0040] One of the mechanisms by which the thermocarbon monoxide-loving Streptomyces CGMCC No. 32349 inhibits Trichoderma pathogens is that its metabolites cause the Trichoderma mycelium to become deformed, the mycelium ends to swell and rupture, and thus inhibit the growth of pathogens (see Figure 4 ).
[0041] Another object of the present invention is to provide a method for culturing thermocarbon monoxide-producing Streptomyces.
[0042] The method for culturing thermocarbon monoxide-loving Streptomyces provided by the present invention comprises the step of culturing the thermocarbon monoxide-loving Streptomyces in a culture medium for culturing microorganisms.
[0043] In the present application, the culture medium for culturing microorganisms may be a solid culture medium, a semi-solid culture medium or a liquid culture medium. The culture medium may contain a carbon source, a nitrogen source, an inorganic salt and trace elements, or any combination thereof. A carbon source is a carbon-containing compound, including sugars, oils, organic acids, organic acid esters and alcohols, etc., or any combination thereof. A nitrogen source refers to a substance that provides nitrogen elements required for microbial nutrition, including peanut meal, soybean meal, soybean meal, yeast powder, peptone, ammonia water, ammonium salts and nitrates, etc., or any combination thereof.
[0044] Furthermore, the culture medium for culturing microorganisms is selected from at least one of PDA, wheat liquid culture medium and Gao's No. 1.
[0045] Another object of the present invention is to provide a method for preparing the above composition.
[0046] The method for preparing the above composition provided by the present invention comprises the step of using the above thermocarbon monoxide-loving Streptomyces as a component of the composition.
[0047] The invention also provides a method for growing edible fungi.
[0048] The method for growing edible fungi provided by the present invention comprises the following steps: preparing a mushroom stick containing thermophilic carbon monoxide streptomyces or the above composition, inoculating edible fungi species, culturing the mushroom sticks, and performing conventional management.
[0049] The edible fungi include one or more of shiitake mushrooms, oyster mushrooms, black fungus, button mushrooms, morels, and stropharia.
[0050] The thermocarbon monoxide streptomyces CGMCC No. 32349 of the present invention has an inhibitory effect on fungal diseases of edible fungi, including Trichoderima koningii, Trichoderma harzianum and Fusarium equiseti, and has no effect on the growth of edible fungi.
[0051] Collection Instructions
[0052] Bacterial species name: Streptomyces thermophilus
[0053] Latin name: Streptomyces thermocarboxydus
[0054] Strain number: P8-1
[0055] Depository: China National Microbiological Culture Collection Administration General Microbiology Center
[0056] Abbreviation of depository institution: CGMCC
[0057] Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing
[0058] Deposit date: October 25, 2024
[0059] CGMCC Registration Number: CGMCC No.32349 BRIEF DESCRIPTION OF THE DRAWINGS
[0060] Figure 1 It is a schematic diagram of the gradient dilution coating separation method in the present invention.
[0061] Figure 2 This is the result of plate separation of Streptomyces strain P8-1.
[0062] Figure 3 The screened Streptomyces P8-1 has an inhibitory effect on pathogenic fungi such as Trichoderma, Fusarium and edible fungi.
[0063] Figure 4 The morphology of mycelium was observed and photographed under a 40x optical microscope.
[0064] Figure 5 The nucleotide sequence of the 16S rRNA gene of the thermophilic carbon monoxide Streptomyces was used for molecular identification, and a phylogenetic tree was constructed using the NJ method.
[0065] Figure 6 The nucleotide sequence of the atpD gene of the thermocarbon monoxide-loving Streptomyces was molecularly identified, and a phylogenetic tree was constructed using the NJ method.
[0066] Figure 7 The nucleotide sequence of the rpoB gene of the thermocarbon monoxide-loving Streptomyces was molecularly identified, and a phylogenetic tree was constructed using the NJ method. DETAILED DESCRIPTION
[0067] The present invention is further described in detail below in conjunction with specific embodiments, and the examples provided are only for illustrating the present invention, rather than for limiting the scope of the present invention. The examples provided below can be used as a guide for further improvements by those of ordinary skill in the art, and do not constitute a limitation of the present invention in any way.
[0068] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial channels.
[0069] The pathogens used in the following examples can be collected from the wild by the public or obtained from the Beijing Academy of Agriculture and Forestry Sciences to repeat the experiments of this application.
[0070] The edible fungi pathogens Trichoderima koningii, T. harzianum and Fusarium equiseti were collected, identified and preserved in the field by the Edible Fungi Research Laboratory, Institute of Plant Protection, Beijing Academy of Agricultural and Forestry Sciences.
[0071] Example 1 Isolation and identification of Streptomyces thermocarboxydus P8-1
[0072] 1. Sample collection
[0073] Collected from the Stropharia officinalis materials in Fangshan District, Beijing.
[0074] 2. Isolation and antagonism screening of strains
[0075] Isolate the strain from the cultivation material. Take 5 grams of fermented edible fungus material (70% poplar wood chips mixed with 30% corn cobs, composted in the open air for 10 days, with the moisture content maintained at 55%-60%, and the fermentation temperature at 55-60°C), dissolve it in 10 ml of sterile water, shake it on a shaker at 180 rpm for 10 min, and separate it by conventional gradient dilution coating (see the method for details). Figure 1 ), LB, NA, PDA and Gao's No. 1 were used to culture at 28°C, and actinomycete colonies with different colony morphology and pure culture were selected, purified and preserved on Gao's No. 1 medium, and antagonistic bacteria were screened and rescreened multiple times using the plate confrontation method with Trichoderma koningii as the target pathogen, and finally an actinomycete strain with strong antibacterial activity was obtained, which was named P8-1. The test results are shown in Figure 2 .
[0076] 3. Strain preservation
[0077] Use glycerol cryopreservation tubes to preserve bacterial strains. Inoculate the flourishing bacterial cells of Gao's No. 1 culture into 20% glycerol sterilized at 121℃ for 30 minutes, and store 4 tubes of each actinomycete. Write the bacterial strain preservation number and date on the cryopreservation tubes, and place the cryopreserved tubes in a -20℃ refrigerator.
[0078] 4. Identification of strains
[0079] The strain P8-1 was analyzed for morphology, physiological and biochemical characteristics and some conservative sequences. The physiological and biochemical and environmental tolerance characteristics were determined according to the methods described in "Principles and Methods of Soil Microbiology Research" (Lin Xiangui. Principles and Methods of Soil Microbiology Research [M], Higher Education Press, 2010).
[0080] The results showed that the bacterial cells of strain P8-1 had 3-6 loose spirals of spores and oblong and oval spores under a 40x optical microscope. On Gao's synthetic medium No. 1, the base silk was milky white and the aerial silk was grayish white; the size of the colony was 1-2 mm, round in shape, with neat edges, opaque, grayish white in color, convex in the middle, bright and smooth on the surface, and dry and hard in texture.
[0081] Figure 4 The morphology of the hyphae was observed and photographed under a 40x optical microscope. As shown in the figure, branched hyphae can be observed. The aerial hyphae are partially differentiated into straight, flexible, hook-shaped, loose or tightly spiral spore threads. The spore chains formed after the spore threads mature are beaded. After the spores mature, they escape from the spore threads and fly away, with a smooth surface.
[0082] The physiological and biochemical characteristics of the strain P8-1 are Gram-positive. On potato culture medium: aerial filaments are gray, basal filaments are fine, colorless or yellow-brown, and the matrix is not stained or brown-yellow.
[0083] The 16S rDNA sequence of strain P8-1 is shown in SEQ ID No.1, and the rpoB gene sequence is shown in SEQ ID No.2.
[0084] SEQ ID No.1:
[0085] GGGGGGTGCTTACACATGCAGTCGAACGATGAACCACTTCGGTGGGGATTAGTGGCGAACGGGTGAGTAACACGTGGGCAATCTGCCCTGCACTCTGGGACAAGCCCTGGAAACGGGGTCTAATACCGGATACTGATCGCCTTGGGCATCCTTGGTGATCGAAAGCTCCGGCGGTGCAGGATGAGCCCGCGGCCTATCAGCTTGTTGGTGAGGTAATGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCGCGTCGGTTGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCAGTCGATACGGGCAGGCTAGAGTTCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCGATACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGA
[0086] SEQ ID No.2
[0087] GCCGCAGACCATGATCACATCCGGCCGGTCGTCGCCTCCATCAAGGAGTTCTTCGGCACCAGCCAGCTGTCCCAGTTCATGGACCAGAACAACCCGCTGTCGGGGCTGACGCACAAGCGTCGTCTGAACGCCCTCGGCCCGGGTGGTCTCTCCCGTGAGCGGGCCGGCTTCGAGGTCCGTGACGTGCACCCCTCGCACTACGGCCGCATGTGCCCGATCGAGACGCCCGAAGGCCCGAACATCGGTCTGATCGGTTCGCTCGCCTCCTACGGGCGGATCAACCCGTTCGGCTTCATCGAGACGCCGTACCGCAAGGTCGTCGACGGCCAGGTCACCGACGAGGTGGACTACCTGACCGCCGACGAGGAGGACCGCTTCGTCATCGCGCAGGCCAACGCGCCGCTCAACGACGAGATGCGCTTCGTCGAGAACCGCATCCTGGTCCGCCGCCGCGGCGGCGAGGTCGACTACGTCCCCGGCGACGAGGTCGACTACATGGACGTCTCGCCGCGCCAGATGGTGTCGGTCGCGACCGCCATGATCCCGTTCCTCGAGCACGACGACGCCAACCGTGCCCTCATGGGCGCGAACATGATGCGCCAGGCCGTGCCGCTGATTAAGTCCGAGGCCCCGCTCGTCGGCACCGGCATGGAGTACCGCTCCGCCGTCGACGCCGGTGACGTGGTCAAGGCGGAGAAGGCGGGCGTGGTCCAGGAGGTCTCCGCGGACTACATCACCACGGCCAACGACGACGGCACGTACATCACGTACCGCCTGGCCAAGTTCTCCCGCTCCAACCAGGGCACCTCGGTCAACCAGAAGGTCATCGTCTCCGAGGGCGACCGCGTCATCGAGGGCCAGGTCCTCGCCGACGGTCCGGCCACCCAGAACGGCGAGATGGCGCTGGGCAAGAACTGCT
[0088] Figure 5The nucleotide sequence of the 16S rRNA gene of the thermophilic carbon monoxide Streptomyces was used for molecular identification, and a phylogenetic tree was constructed using the NJ method.
[0089] Figure 6 The nucleotide sequence of the atpD gene of the thermocarbon monoxide-loving Streptomyces was molecularly identified, and a phylogenetic tree was constructed using the NJ method.
[0090] Figure 7 The nucleotide sequence of the rpoB gene of the thermocarbon monoxide-loving Streptomyces was molecularly identified, and a phylogenetic tree was constructed using the NJ method.
[0091] Example 2: Fermentation and preparation of bacterial agent of strain P8-1
[0092] Medium preparation
[0093] PD: 200g potatoes, clean and cut into 1cm pieces 3 Boil the small pieces for about 30 minutes and filter them through four layers of gauze. Make up the filtrate to 1000ml with distilled water, add 20g glucose, and set the pH to natural. Stir evenly, sterilize at 120℃, 0.1MP for 30 minutes, and cool for use.
[0094] PDA: 200g potatoes, clean and cut into 1cm pieces 3 Boil the small pieces for about 30 minutes and filter them through four layers of gauze. Make up the filtrate to 1000ml with distilled water, add 20g glucose and 15g agar, and set the pH to natural; stir evenly, sterilize at 120℃, 0.1MP for 30 minutes, and cool for use.
[0095] Wheat liquid culture medium: Weigh 60g of wheat, break the wheat wall with a wall breaker, then put it into 1000ml of distilled water, add 0.01g of FeSO4﹒7H2O, 0.50g of K2HPO4, and 0.50g of MgSO4﹒7H2O, stir evenly, sterilize at 120℃, 0.1MP, for 30min, and cool for use.
[0096] The screened antagonistic bacterial strain P8-1 was inoculated onto a PDA plate by a coating method, placed in an incubator, and cultured at 25°C for 4 days. The plate was then inoculated into a sterilized conical flask containing 200 mL of liquid PD culture medium and cultured at 25°C and 200 r / min for 4 days. The culture was stopped when the colony count reached 100 million CFU / mL. The plate was then inoculated into a 5 L wheat liquid culture medium, 0.5% Tween-80 was added, and the plate was mixed for later use.
[0097] Example 3: Inhibition of Streptomyces P8-1 on pathogenic fungi Trichoderma, Fusarium and edible fungi
[0098] Use a 6mm diameter puncher to punch fresh fungus cakes on the activated green Trichoderma plates, Fusarium equisetifolia plates, Stropharia rugosodium plates and Pleurotus ostreatus plates, and then invert them in the center of the new PDA medium plate. Then pick the purified biocontrol strains and draw a vertical line of about 30mm in the center of the medium 20mm away from the Trichoderma cake. Set up 3 replicates for each test bacterium. All plates were cultured at a constant temperature of 28℃ for 3-4 days, and the mycelial growth was observed and recorded to clarify the effect of actinomycete P8-1 on the mycelial growth of green Trichoderma, Fusarium equisetifolia and Stropharia rugosodium.
[0099] Figure 3 The results show that the strain P8-1 has obvious inhibitory effects on the pathogens Trichoderma and Fusarium (inhibition zone in the figure), but has no effect on Pleurotus ostreatus and Pleurotus ostreatus.
[0100] Example 4: Detection of the control effect of strain P8-1 on Trichoderma ostreatus and other diseases
[0101] The thermophilic carbon monoxide Streptomyces thermocarboxydus P8-1 prepared in Example 2 was diluted 100 times with tap water and evenly sprayed on the oyster mushroom material (45% cottonseed hulls, 45% corn cobs, 8% bran, 2% lime) (the final concentration of P8-1 was between 100 million CFU / g and 100 million CFU / g), and the pile was piled at room temperature for 7 days. During this period, the pile was turned once when the temperature rose to 50°C, and then bagged to make sticks (1 kg of dry material per stick, 70% water content), sterilized (normal pressure sterilization: 90-100°C, 10 hours), inoculated with branch species (oyster mushroom variety: 969) in an open environment, and then cultured at 25°C for bacteria sticks, and conventional management; under the same conditions, the bacteria sticks without adding P8-1 bacterial agent were used as controls. Each treatment had 100 sticks, and the contamination of the bacteria sticks by Trichoderma and other fungi was counted after 30 days of culture. The test results show that the application of the P8-1 strain of the present invention can significantly inhibit the occurrence of diseases such as Trichoderma and can significantly reduce the loss of mushroom stick yield caused by contaminating bacteria (Table 1).
[0102] Table 1. Inhibition of the thermocarboxydus strain P8-1 of the present invention on diseases of Trichoderma ostreatus and the like and its effect on the ostreatus mushroom sticks
[0103]
[0104] **CK control refers to the routine operation of Pleurotus ostreatus materials without the addition of bacterial agent P8-1; bacterial agent P8-1 treatment refers to the addition of bacterial agent P8-1 to Pleurotus ostreatus materials.
[0105] *The contamination rate of mushroom sticks refers to the ratio of the number of mushroom sticks infected by Trichoderma, Chlorella, Fusarium, etc. to the total number of mushroom sticks during the fermentation process.
[0106] The present invention has been described in detail above. For those skilled in the art, without departing from the purpose and scope of the present invention, and without the need to carry out unnecessary experimental conditions, the present invention can be implemented in a wide range under equivalent parameters, concentrations and conditions. Although the present invention provides specific embodiments, it should be understood that the present invention can be further improved. In a word, according to the principles of the present invention, the application is intended to include any changes, uses or improvements to the present invention, including departure from the disclosed scope in the application, and changes made with conventional techniques known in the art.
Claims
1. Streptomyces, characterized in that: The streptomyces is Streptomyces thermocarboxydus, the strain number of which is P8-1, and the registration number of which in the General Microbiological Center of China National Microbiological Culture Collection is CGMCC No.32349.
2. A composition containing the thermophilic carboxytopenia bacteria according to claim 1, wherein the active ingredient of the composition is the thermophilic carboxytopenia bacteria and / or its metabolites and / or its culture.
3. The composition according to claim 2, characterized in that: The culture is a substance obtained by culturing the thermocarbon monoxide-loving Streptomyces in a microbial culture medium.
4. The composition according to claim 2, characterized in that: The composition is a bacterial agent and a microecological preparation.
5. The use of the Streptomyces according to claim 1, wherein the use is any one of the following: 1) Inhibit pathogens; 2) Preparation of pathogen inhibitors; 3) Suppress diseases; 4) Preparation of disease inhibitors.
6. The use of the composition according to any one of claims 2 to 4, wherein the use is any one of the following: 1) Inhibit pathogens; 2) Preparation of pathogen inhibitors; 3) Suppress diseases; 4) Preparation of disease inhibitors.
7. The use according to claim 5 or 6, characterized in that: The pathogen is at least one of the following: Trichoderma and Fusarium; The disease is a fungal disease of edible fungi, specifically, at least one of a Trichoderma disease and a Fusarium disease of edible fungi; The edible fungi include one or more of shiitake mushrooms, oyster mushrooms, black fungus, button mushrooms, morels, and stropharia.
8. A method for culturing thermophilic carbon monoxide-producing Streptomyces, characterized in that: The thermophilic carboxytopenia streptomyces is the thermophilic carboxytopenia streptomyces according to claim 1, and the method comprises the step of culturing the thermophilic carboxytopenia streptomyces in a culture medium for culturing microorganisms.
9. A method for preparing the composition according to any one of claims 2 to 4, comprising the step of using the thermocarbon monoxide-loving Streptomyces according to claim 1 as a component of the composition.
10. A method for growing edible fungi, characterized in that: The method comprises the following steps: preparing a mushroom stick containing the thermophilic carboxygenase Streptomyces according to claim 1 or containing the composition according to any one of claims 2 to 4, inoculating edible mushroom species, culturing the mushroom sticks, and performing conventional management.