Fish test water tank

Abstract

The invention belongs to the field of fish research equipment, and discloses a fish test water tank which comprises a water tank body, an inner-layer net cage, a hanging bracket, a moving trolley and an aeration pipe. The water tank body is internally provided with a space required by fish tests and sufficient water body capacity, so that a sufficient living environment is provided for tested fishes. The inner-layer mesh cage is arranged in the water tank body and used for limiting the activity space of the tested fishes, and the tested fishes can only swim in the space of the inner-layer mesh cage. The hanging bracket is arranged on the water tank body in a crossing mode, and the inner-layer mesh cage is hung on the hanging bracket, so that the inner-layer mesh cage and the water tank body are arranged at intervals. And the interval between the inner-layer mesh cage and the water tank body provides a placement space for test equipment. The moving trolley is movably arranged on the water tank body in the circumferential direction of the water tank body and carries and drives various test instruments to move around the water tank body. The aeration pipe is mounted on the moving trolley, and the moving trolley drives the aeration pipe to move in the water tank body so as to observe the influence of the bubble curtain on fish behaviors.

Description

Technical Field

[0001] The present invention belongs to the technical field of fish research equipment, and more specifically, relates to a fish test water tank. Background Art

[0002] In aquaculture and fishery resource management, the bubble curtain, as an important technical facility, plays a significant role in controlling fish behavior through its unique physical effects. The bubble curtain is mainly composed of perforated air pipes arranged at the bottom of the water body. These air pipes continuously discharge compressed air, thereby generating a dense bubble group from bottom to top in the water body. This process not only produces significant sound and low-frequency pressure vibrations in the water body but also forms an upward flowing water current. These combined effects act on fish together, affecting their behavior patterns from multiple dimensions.

[0003] Specifically, the sound waves and vibrations of the bubble curtain can arouse the vigilance of fish. Especially when a fish school approaches, this strong stimulation often causes the fish to change their swimming directions, thus playing a role in blocking or driving them to a certain extent. At the same time, the water current formed during the rising of the bubbles also generates a certain driving force on the fish. This physical force can guide the fish school to change its swimming trajectory or speed in some cases. Therefore, the bubble curtain shows broad application prospects in preventing the escape of aquaculture animals, guiding the migration of fish schools, and protecting the ecological balance of specific water areas.

[0004] However, although the bubble curtain technology has been widely applied in practice, the in-depth research on its impact on fish behavior lags behind relatively. Currently, there is a lack of test water tanks dedicated to studying the bubble curtain effect in the market, which to a certain extent restricts the in-depth development of related scientific research work. The traditional water tank design often focuses on the static or simple flowing state of the water body and is difficult to simulate the complex physical scenarios generated by the bubble curtain. Therefore, it cannot meet the needs of accurately studying the impact of the bubble curtain on fish behavior. Summary of the Invention

[0005] The purpose of the embodiments of the present application is to provide a fish test water tank to solve the technical problem that the existing water tank cannot meet the research requirements in the prior art.

[0006] To achieve the above purpose, the technical solution adopted in the present application is: Provide a fish test water tank, including: A water tank body; An inner wire cage, arranged in the water tank body; A hanger, spanning across the water tank body, the inner wire cage is suspended on the hanger, and the inner wire cage is spaced from the water tank body; A mobile trolley, movably arranged on the water tank body along the circumferential direction of the water tank body; The aeration pipe is installed on the mobile trolley, and the aeration end of the aeration pipe is arranged at the interval between the water tank body and the inner wire cage.

[0007] As a further improvement of the above technical solution: Optionally, guide rails are provided on the water tank body, the guide rails are arranged along the circumferential direction of the water tank body, and the mobile trolley is movably connected to the guide rails.

[0008] Optionally, the mobile trolley includes a vehicle body and a suspension platform. The vehicle body is movably arranged on the water tank body along the circumferential direction of the water tank body. The suspension platform is connected to one side of the vehicle body and is located outside the side wall of the water tank body.

[0009] Optionally, the mobile trolley further includes a rotating arm, and the rotating arm is rotatably connected to the vehicle body.

[0010] Optionally, the water tank body is an annular water tank.

[0011] Optionally, mobile trolleys operating independently are provided on both the inner ring side wall and the outer ring side wall of the annular water tank.

[0012] Optionally, the water tank body includes a tank body frame and an observation window. The observation window is connected to the tank body frame and is located on the side wall of the water tank body.

[0013] Optionally, the inner wire cage includes an inner wire frame, a light-transmitting window, and a water-permeable net. The light-transmitting window is connected to the inner wire frame and is located on the side of the inner wire cage. The water-permeable net is connected to the bottom surface of the inner wire frame.

[0014] This application also provides a test method based on the above fish test water tank, including the following steps: Place a predetermined number of test fish in the inner wire cage of the water tank body; Inject gas into the aeration pipe to form a bubble curtain in the inner wire cage; Control the mobile trolley to drive the aeration pipe to move along the guide rail, and monitor the noise volume generated by aeration and the behavior of the test fish; By controlling the different gas injection volumes in the aeration pipe, the noise volume generated by aeration, and the different moving speeds of the mobile trolley, record the driving effect on the test fish; Under the condition that the gas injection volume in the aeration pipe and the noise volume generated by aeration are certain, control the mobile trolleys on the inner ring side wall and the outer ring side wall to move towards each other, observe the behavior of the test fish in front of the bubble curtain, and obtain the minimum clamping distance of the bubble curtain on the test fish according to the behavior of the test fish; Under the condition that the air injection volume in the aeration pipe and the noise volume generated by aeration are constant, the test fish is clamped into a predetermined interval, and the moving carts on the inner ring sidewall and the moving carts on the outer ring sidewall are controlled to move in the same direction at a predetermined speed, and the transfer effect on the test fish is recorded.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: This application provides a fish test water tank, including a water tank body, an inner layer cage, a hanger, a moving cart and an aeration pipe. Among them, the water tank body is the outermost structure of the fish test water tank. The water tank body has the space required for fish tests and sufficient water volume, providing a sufficient living environment for the test fish. The inner layer cage is arranged in the water tank body. The inner layer cage is used to limit the activity space of the test fish, and the test fish can only swim within the space of the inner layer cage. Sufficient living space also needs to be ensured within the inner layer cage for the test fish. The hanger straddles the water tank body, and the inner layer cage is suspended in the water tank body through the hanger, so that the inner layer cage is spaced from the water tank body. The interval between the inner layer cage and the water tank body provides an installation space for test equipment, reducing the interference of test equipment on fish and ensuring that only the influencing sources provided by the test affect fish behavior. The moving cart is movably arranged on the water tank body along the circumference of the water tank body; the moving cart is used to carry various test equipment and drive various test equipment to move around the water tank body. The aeration pipe is a test component for generating a bubble curtain, and the bubble curtain can penetrate through the inner layer cage, thereby affecting fish behavior. The aeration pipe is installed on the moving cart, and the moving cart drives the aeration pipe to move in the water tank body to observe the influence of the bubble curtain on fish behavior. The aeration end of the aeration pipe is arranged at the interval between the water tank body and the inner layer cage, which can prevent the fish group from colliding with the aeration pipe and reduce the interference of the aeration pipe on fish, ensuring that only the bubble curtain affects fish behavior. Description of the Drawings

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0017] Figure 1 is a top view structural schematic diagram of the fish test water tank of this application; Figure 2 is a cross-sectional structural schematic diagram of the fish test water tank of this application; Figure 3 is a partial enlarged structural schematic of the fish test water tank of this application Figure 1 ; Figure 4It is a schematic diagram of a partially enlarged structure of the fish test water tank of the present application Figure 2 ; Figure 5 It is a schematic diagram of a partially enlarged structure of the fish test water tank of the present application Figure 3 。

[0018] Among them, the reference numerals in the figure are as follows: 1. Water tank body; 11. Inner ring side wall; 12. Outer ring side wall; 13. Tank body frame; 14. Observation window; 2. Inner layer cage; 21. Inner network frame; 22. Transparent window; 23. Permeable net; 3. Hanger; 4. Mobile trolley; 41. Vehicle body; 42. Suspension platform; 43. Rotary arm; 5. Aeration pipe; 6. Guide rail. Specific implementation manners

[0019] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application clearer and more understandable, the present application will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0020] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0021] It should be understood that the orientation or positional relationship indicated by the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present application.

[0022] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present application, "a plurality of" means two or more unless otherwise specifically defined.

[0023] Unless otherwise defined, all technical terms used hereinafter have the same meaning as commonly understood by those skilled in the art. The technical terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present invention.

[0024] As Figures 1 to 5 shown, the present application provides a fish test water tank, which includes a water tank body 1, an inner cage 2, a hanging bracket 3, a mobile trolley 4, and an air diffuser pipe 5. Among them, the water tank body 1 is the outermost structure of the fish test water tank. The water tank body 1 has the space required for fish tests and sufficient water volume to provide a sufficient living environment for the test fish. The inner cage 2 is arranged in the water tank body 1. The inner cage 2 is used to limit the activity space of the test fish, and the test fish can only swim within the space of the inner cage 2. Sufficient living space also needs to be ensured within the inner cage 2 for the test fish. The hanging bracket 3 straddles the water tank body 1, and the inner cage 2 is suspended in the water tank body 1 through the hanging bracket 3, so that the inner cage 2 and the water tank body 1 are arranged at intervals. The interval between the inner cage 2 and the water tank body 1 provides an installation space for test equipment, so as to reduce the interference of the test equipment on the fish and ensure that only the influencing sources provided by the test affect the fish behavior. The mobile trolley 4 is movably arranged on the water tank body 1 along the circumferential direction of the water tank body 1; the mobile trolley 4 is used to carry various test equipment and drive various test equipment to move around the water tank body 1. The air diffuser pipe 5 is a test component for generating a bubble curtain influencing source, and the bubble curtain can pass through the inner cage 2, thereby affecting the fish behavior. The air diffuser pipe 5 is installed on the mobile trolley 4, and the mobile trolley 4 drives the air diffuser pipe 5 to move in the water tank body 1 to observe the influence of the bubble curtain on the fish behavior. The aeration end of the air diffuser pipe 5 is arranged at the interval between the water tank body 1 and the inner cage 2, which can not only prevent the fish group from colliding with the air diffuser pipe 5, but also reduce the interference of the air diffuser pipe 5 on the fish, ensuring that only the bubble curtain affects the fish behavior.

[0025] As Figure 2 shown, in an embodiment of the present application, a guide rail 6 is arranged on the water tank body 1. The guide rail 6 is arranged along the circumferential direction of the water tank body 1, and the mobile trolley 4 is movably connected to the guide rail 6. Specifically, the guide rail 6 is arranged on the top of the water tank body 1 to facilitate the movement of the mobile trolley 4 around the water tank body 1. The guide rail 6 provides a stable and reliable movement path for the mobile trolley 4 to ensure that the mobile trolley 4 can move along the established route.

[0026] As Figure 2As shown, in an embodiment of the present application, the mobile trolley 4 includes a vehicle body 41 and a suspension platform 42. The vehicle body 41 is movably arranged on the water tank body 1 along the circumferential direction of the water tank body 1. The vehicle body 41 is connected to the guide rail 6 through the rollers at the bottom to reduce the resistance and energy consumption during the movement of the vehicle body 41. The suspension platform 42 is connected to one side of the vehicle body 41 and is located outside the side wall of the water tank body 1. A variety of test components such as test instruments or counterweights can be carried on the suspension platform 42. Through the test instruments, the behavior of fish in the inner cage 2 under the influence of the influence source provided in the test can be recorded; the counterweight can be used to balance the weight of the aeration pipe 5 on the other side, effectively preventing the mobile trolley 4 from tipping over due to uneven load during operation, ensuring the safety and stability of the entire system.

[0027] As Figure 2 shown, in an embodiment of the present application, the mobile trolley 4 further includes a rotating shaft and a rotating arm 43. The rotating shaft is rotatably connected to the vehicle body 41, and the rotating arm 43 is connected to the rotating shaft, so as to drive the equipment hoisted on the rotating arm 43 to move to any angle. After the rotating shaft is fastened, the rotating arm 43 will be fixed at a predetermined angle. The rotating arm 43 is arranged at the top of the mobile trolley 4. One end of the rotating arm 43 can hoist a light source or an underwater speaker, so as to form a light influence source or a noise influence source. The above types of influence sources can be turned on separately or used in combination during actual tests. A recorder can also be hoisted on this end of the rotating arm 43 to photograph the behavior of fish in the water tank from above the water tank; or an illuminometer can be hoisted to measure the brightness generated by the light influence source; or a hydrophone can be hoisted to measure the sound signal generated by the bubble curtain or the noise influence source; or a laser sheet light source can be hoisted, and an image recording device can be carried on the suspension platform 42 at the same time to form a particle image velocimetry system (PIV). Using this system, the water flow velocity field around the bubble curtain can be measured. The other end of the rotating arm 43 can also carry a workbench, and a variety of test components such as test instruments or counterweights can be carried on the workbench.

[0028] As Figure 1 shown, in an embodiment of the present application, the water tank body 1 is specifically an annular water tank, so as to provide a space for fish to swim in a cycle. Similarly, the inner cage 2 also needs to be set as an annular cage to cooperate with the annular water tank to provide a space for fish to swim in a cycle, so that the test fish can swim continuously unobstructed in the inner cage 2.

[0029] As Figure 1 and Figure 4As shown in the figure, in an embodiment of the present application, moving trolleys 4 that operate independently are provided on both the inner ring side wall 11 and the outer ring side wall 12 of the annular water tank. These two moving trolleys 4, as two independent working units, operate independently of each other without interference, and can form two independent and controllable bubble curtains inside the water tank, so as to adjust the position, intensity and distribution of the bubble curtains according to different test requirements, and provide a variety of test modes.

[0030] In addition, each moving trolley 4 can also carry a variety of different test equipment, which not only improves the multi-functional test performance of the test water tank, but also increases the redundancy and reliability of the equipment. During the test, even if a certain moving trolley 4 or a certain test equipment fails, it will not have a serious impact on the entire test, thus ensuring the continuity and stability of the test.

[0031] As Figure 5 shown in the figure, in an embodiment of the present application, the water tank body 1 includes a tank body frame 13 and an observation window 14. The tank body frame 13 serves as the support skeleton of the water tank body 1 and can be specifically constructed by using a high-strength steel structure or a concrete structure with strong durability. The tank body frame 13 should be able to bear the weight of the water body, the moving trolley 4 and other test equipment. The observation window 14 is installed on the tank body frame 13 and is located on the side wall of the water tank body 1. The observation window 14 is composed of transparent tempered glass or plexiglass, which can ensure that scientific researchers can clearly observe the behavior of the fish inside the water tank. Through the observation window 14, key information such as the swimming trajectory and behavior pattern of the fish can be intuitively monitored, providing data support for fish behavior research.

[0032] As Figure 5 shown in the figure, in an embodiment of the present application, the inner layer cage 2 includes an inner net frame 21, a light-transmitting window 22 and a water-permeable net 23. The inner net frame 21 can be constructed by using a lightweight steel structure or an aluminum alloy structure to reduce the load on the hanger 3. The light-transmitting window 22 is connected to the inner net frame 21 and is located on the side of the inner layer cage 2. The light-transmitting window 22 is composed of transparent tempered glass, plexiglass or mesh to intuitively monitor the behavior of the fish. The water-permeable net 23 is connected to the bottom surface of the inner net frame 21. The water-permeable net 23 needs to ensure the smooth passage of the bubble curtain and be able to intercept the test fish to prevent the test fish from escaping to the space between the water tank body 1 and the inner layer cage 2.

[0033] The present application also provides a test method for the fish test water tank in the above embodiment. The specific test steps are as follows: Place a predetermined number of test fish in the inner cage 2 of the water tank body 1, and the number usually ranges from 10 to 40 to ensure the statistical significance and representativeness of the test results. Subsequently, compressed air is injected through the air diffuser pipe 5, and the gas flow rate is controlled between 20 L / min and 160 L / min per unit width to form a uniform and stable bubble curtain in the inner cage 2. To precisely control the test conditions, the first moving cart 4 moves along the guide rail 6, driving the air diffuser pipe 5 to move at a constant speed or variable speed. During this process, the noise volume generated by aeration is monitored in real time, and the behavior changes of the test fish, including swimming trajectories, group distributions, and stress responses, are recorded by a high-definition camera device. At the same time, the second moving cart 4 maintains a certain distance in front of the traveling direction of the first moving cart 4, and this distance is usually 1 to 4 times the width of the water tank. A high-sensitivity hydrophone is suspended on the second moving cart 4 through the swing arm 43 to measure the noise spectrum and sound pressure level generated by the bubble curtain, and the data is transmitted to the recorder in real time through a wireless transmission system. Both moving carts 4 are equipped with high-precision recorders to synchronously record the behavior data of the fish near and in front of the bubble curtain. By comparing and analyzing the fish behavior under different air injection volumes, noise volumes, and moving speeds, the driving effect of the bubble curtain on the test fish can be systematically evaluated. During the test process, the air injection volume and moving speed can be adjusted according to the test requirements.

[0034] In an embodiment of the present application, under the condition that the air injection volume in the air diffuser pipe 5 and the noise volume generated by aeration remain constant, the moving cart 4 on the inner ring sidewall 11 and the moving cart 4 on the outer ring sidewall 12 are controlled to move in an approaching manner, and at the same time, the behavior changes of the test fish in front of the bubble curtain are observed in real time. By recording the behavior characteristics of the fish, such as swimming trajectories, group distributions, and stress responses, during the approaching process of the bubble curtain, the minimum pinching distance at which the bubble curtain has an effective driving effect on the test fish is analyzed and determined. The determination of this minimum pinching distance is based on the behavior threshold of the fish when the bubble curtain approaches, such as the distance when the fish starts to significantly change its swimming direction or accelerate to escape. The moving speed of the moving cart 4 adopts the optimal moving speed measured through experiments in the above embodiment to ensure the scientific nature and repeatability of the test conditions.

[0035] In one embodiment of the present application, under the condition that the gas injection volume in the aeration pipe 5 and the noise volume generated by aeration remain constant, the test fish are driven and clamped into a predetermined area within the water tank body 1 to ensure the controllability and consistency of the test environment. Subsequently, the moving trolley 4 on the inner ring side wall 11 and the moving trolley 4 on the outer ring side wall 12 are controlled to move in the same direction at the same predetermined speed to simulate the continuous driving effect of the bubble curtain on the fish in actual application. During this process, data such as the swimming trajectories, group distributions, behavioral changes, and acoustic responses of the test fish are monitored and recorded in real time through high-definition imaging devices and hydrophones to evaluate the transfer effect of the bubble curtain on the fish. The predetermined speed of the moving trolley 4 is optimized based on the previous test data to ensure the maximization of the driving efficiency and the minimization of the stress response of the fish. By systematically analyzing the transfer behavior of the test fish under the action of the bubble curtain, the driving efficiency of the bubble curtain can be accurately quantified, providing a scientific basis for parameter optimization in actual applications.

[0036] In other embodiments of the present application, a light source or an underwater speaker can be further mounted on the moving trolley 4 as an additional influencing source to study the behavioral responses of the test fish under different environmental stimulation conditions. The light source can adopt an LED lamp group with different wavelengths and intensities to simulate the light environment under natural light or specific spectral conditions; the underwater speaker can play sound signals with specific frequencies and sound pressure levels to simulate underwater noise or specific acoustic environments. By controlling parameters such as the switching frequency, light intensity of the light source, or the sound frequency and sound pressure level of the underwater speaker, the behavioral changes of the test fish under the action of multiple influencing sources are studied, including but not limited to swimming trajectories, group distributions, phototaxis, or sound avoidance responses, etc., so as to more realistically simulate the actual application scenario and provide a basis for optimizing the driving strategy.

[0037] The above are only the preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A fish test tank, characterized in that: include: Sink body (1); An inner mesh cage (2) is arranged in the water tank body (1); A hanger (3) is straddled on the water tank body (1), and the inner mesh cage (2) is hung on the hanger (3), and the inner mesh cage (2) is spaced apart from the water tank body (1); A movable trolley (4) is movably arranged on the water tank body (1) along the circumference of the water tank body (1); An aeration pipe (5) is installed on the mobile trolley (4), and an aeration end of the aeration pipe (5) is arranged at the interval between the water tank body (1) and the inner mesh cage (2).

2. The fish test tank according to claim 1, characterized in that: The water tank body (1) is provided with a guide rail (6), the guide rail (6) being arranged along the circumference of the water tank body (1), and the movable trolley (4) is movably connected to the guide rail (6).

3. The fish testing tank according to claim 1, characterized in that: The movable vehicle (4) comprises a vehicle body (41) and a hanging platform (42); the vehicle body (41) is movably arranged on the water tank body (1) along the circumference of the water tank body (1); and the hanging platform (42) is connected to one side of the vehicle body (41) and is located outside the side wall of the water tank body (1).

4. The fish testing tank according to claim 3, characterized in that: The mobile vehicle (4) further comprises a rotary arm (43), wherein the rotary arm (43) is rotatably connected to the vehicle body (41).

5. The fish testing tank according to any one of claims 1 to 4, characterized in that: The water tank body (1) is an annular water tank.

6. The fish testing tank according to claim 5, characterized in that: An independently movable trolley (4) is provided on both the inner ring side wall (11) and the outer ring side wall (12) of the annular water tank.

7. The fish testing tank according to any one of claims 1 to 4, characterized in that: The water tank body (1) comprises a tank frame (13) and an observation window (14); the observation window (14) is connected to the tank frame (13) and is located on a side wall of the water tank body (1).

8. The fish testing tank according to any one of claims 1 to 4, characterized in that: The inner mesh cage (2) comprises an inner mesh frame (21), a light-transmitting window (22) and a water-permeable net (23); the light-transmitting window (22) is connected to the inner mesh frame (21) and is located on a side surface of the inner mesh cage (2); and the water-permeable net (23) is connected to the bottom surface of the inner mesh frame (21).

9. A test method based on the fish test tank according to any one of claims 1 to 8, characterized in that: The steps include: A predetermined number of test fish are placed in an inner net cage (2) of a water tank body (1); Injecting gas into the aeration pipe (5) to form a bubble curtain in the inner mesh cage (2); Controlling the mobile trolley (4) to drive the aeration pipe (5) to move along the guide rail (6), and monitoring the amount of noise generated by aeration and the behavior of the test subject fish; By controlling different air injection volumes in the aeration tube (5), the amount of noise generated by aeration, and different moving speeds of the moving cart (4), the repelling effect on the test object fish is recorded; Under the condition that the amount of air injected into the aeration pipe (5) and the amount of noise generated by aeration are constant, the moving trolley (4) on the inner ring side wall (11) and the moving trolley (4) on the outer ring side wall (12) are controlled to move towards each other, and the behavior of the test fish in front of the bubble curtain is observed, and the minimum clamping distance of the bubble curtain on the test fish is obtained according to the behavior of the test fish; Under the condition that the amount of air injected into the aeration pipe (5) and the amount of noise generated by aeration are constant, the test fish are forced into a predetermined interval, the moving trolley (4) on the inner ring side wall (11) and the moving trolley (4) on the outer ring side wall (12) are controlled to move in the same direction at a predetermined speed, and the transfer effect on the test fish is recorded.

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