A microecological preparation for temporary rearing of whiteleg shrimp after capture, and its preparation method and application

By fermenting the microecological preparations of Lactobacillus mucilaginosus RC3 and Lactobacillus plantarum B6, the problems of water pollution and intestinal disorders in the temporary rearing of white shrimp after capture were solved, and the antioxidant enhancement, immune improvement and intestinal health of the shrimp were achieved, and the survival rate and muscle quality were improved.

CN120118811BActive Publication Date: 2025-09-23NINGBO UNIV
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Patent Information

Application Number
CN202510608585.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-05-13
Publication Date
2025-09-23
Estimated Expiration
2045-05-13

AI Technical Summary

Technical Problem

Existing probiotic preparations cannot effectively reduce the nitrite nitrogen and ammonia nitrogen content in the aquaculture water during the temporary rearing of whiteleg shrimp after capture, improve the antioxidant and immune functions of shrimp, optimize the intestinal flora, and are insufficient in the shrimp's own immune repair and intestinal microecological reconstruction.

Method used

The microecological preparations of Limosilactobacillus fermentum RC3 strain and Lactiplantibacillus plantarum B6 strain were mixed and inoculated into MRS liquid culture medium to prepare a microecological preparation for temporary rearing of whiteleg shrimp after capture, thereby increasing the number of viable bacteria and applying it to aquaculture seawater.

Benefits of technology

Significantly reduce the levels of nitrite nitrogen and ammonia nitrogen in aquaculture water, improve the antioxidant capacity of shrimp, enhance immune function, optimize intestinal flora, improve the survival rate and muscle quality of shrimp, and inhibit the growth of potential pathogenic bacteria.

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Abstract

The invention discloses a probiotic preparation for post-harvest temporary aquaculture of whiteleg shrimp (Penaeus vannamei), a preparation method thereof, and an application thereof. The probiotic preparation is characterized in that the probiotic preparation comprises at least one of a fermented Lactobacillus mucilaginosus RC3 strain and a Lactobacillus plantarum B6 strain, the fermented Lactobacillus mucilaginosus RC3 having a preservation number of CGMCC No. 30602, and the Lactobacillus plantarum B6 having a preservation number of CGMCC No. 30603. The probiotic preparation is prepared by mixing a fermented Lactobacillus mucilaginosus RC3 seed liquid and a Lactobacillus plantarum B6 seed liquid in a volume ratio of (1-2):(1-2), and then inoculating the mixture into an MRS liquid culture medium in a volume ratio of 5-10% to obtain the probiotic preparation for post-harvest temporary aquaculture of whiteleg shrimp. The probiotic preparation has the advantages of reducing the contents of nitrite nitrogen and ammonia nitrogen in aquaculture water, improving the antioxidant and immune properties of shrimp, optimizing intestinal flora, and improving the muscle quality of shrimp.
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