Composition for relieving physical fatigue of human body and application and product thereof

Abstract

The invention provides a composition for relieving physical fatigue of a human body as well as application and a product thereof. The composition for relieving the physical fatigue of the human body comprises a water-soluble tomato concentrate, a ginkgo leaf extract and a ginseng extract. Experimental data show that the composition of the water-soluble tomato concentrate, the ginkgo biloba extract and the ginseng extract is compounded for use, so that a synergistic interaction effect can be achieved under the conditions of low dosage and high dosage, the content of serum urea and blood lactic acid of mice can be reduced, the reserve of hepatic glycogen is improved, and the load swimming time of the mice is prolonged; the function of relieving physical fatigue is realized. The composition for relieving the physical fatigue of the human body is applied to the product for relieving the physical fatigue of the human body to obtain the product capable of relieving the physical fatigue of the human body, and the product can be prepared into tablets, capsules, powder, granules, pills or liquid, can be used as a medicine or health-care food and is used for regulating the sub-health of the human body.

Description

Technical Field

[0001] The present invention belongs to the technical fields of health care and medicine, and relates to a composition for relieving physical fatigue of the human body. Specifically, it relates to a composition for relieving physical fatigue of the human body, its application and products. Background Art

[0002] Fatigue is a very complex physiological and psychological phenomenon. It is not composed of a single, clear factor, and there are also great differences in the definitions of fatigue by different institutions or individuals at present. Generally speaking, in the process of production and life, due to changes in physiological and psychological states, the natural exhaustion state of the strength of one or some organs or even the whole body of a person is called fatigue. According to the causes, manifestations, and occurrence sites of fatigue, it can be divided into different types of fatigue.

[0003] Physical fatigue, also known as exercise-induced fatigue, mostly refers to the fatigue feeling of the body caused by insufficient bioenergy reserve or excessive bioenergy consumption when a person is faced with heavy physical labor or large amounts of exercise. It is manifested as soreness, softness, pain, fatigue and weakness, and "being unable to do as one wishes" all over the body or in local areas. Long-term physical fatigue will endanger the normal physiological functions of the human body, lead to endocrine disorders and a decline in immunity, and then organic diseases will appear, affecting health. In recent years, with the acceleration of the pace of life and increasingly fierce competition, the insufficient reserve of bioenergy substances due to sleep problems, or the excessive digestion of bioenergy substances caused by long-term and high-intensity labor, has led to the fatigue phenomenon almost covering all social strata. Therefore, the research and development of functional foods with the function of relieving physical fatigue has positive significance for health promotion.

[0004] The patent with the publication number CN107981127A discloses an anti-fatigue dietary fiber beverage and its preparation method. The dietary fiber beverage is prepared from water-soluble dietary fiber, xylitol, chitosan, lemon juice, papaya juice and tomato juice. Among them, the water-soluble dietary fiber is prepared from apple peel, bean dregs, corn, celery and bamboo. This dietary fiber not only contains rich multiple fiber components, but also contains rich components such as anthocyanins, vitamin C and flavonoids, which can effectively scavenge free radicals in the human body, block the peroxidation of polyunsaturated fatty acids on the biomembrane caused by free radicals, protect body tissues, effectively delay the occurrence of fatigue, and play an anti-fatigue role.

[0005] The patent with the publication number CN106723068A discloses a formula for sea cucumber lycopene selenium anti-radiation capsules and its preparation process. The formula for sea cucumber lycopene selenium anti-radiation capsules is composed of sea cucumber collagen, seabuckthorn extract, rhodiola rosea, VC, VE, grape seed PE, ligustrum lucidum, lycopene, ginseng, selenium-enriched carrageenan, tremella, procyanidins, ganoderma lucidum, β-carotene, ginkgo leaf PE, phyllanthus emblica and spirulina, and has effects such as anti-radiation, enhancing immunity, removing garbage in the body, anti-aging and restoring fatigue.

[0006] The patent with the publication number CN102698001A discloses a health food for hemiplegia rehabilitation. The health food is made of total ginsenosides, total astragalus saponins, gastrodin, pachyman polysaccharide, ginkgo biloba extract, lycopene, volatile oil of acorus tatarinowii, ganoderma spore oil and borneol, and has a significant anti-hemiplegia effect clinically.

[0007] Generally speaking, although a large number of products for relieving physical fatigue are involved in the prior art, the formulations of a large number of products are complex, and the functions of the products have not been scientifically and effectively verified. Therefore, it is of practical significance to develop a product with simple ingredients, wide application range and scientific and effective fatigue relief. Summary of the Invention

[0008] In view of the problems existing in the prior art, the present invention provides a composition for relieving human physical fatigue, its application and product. The present invention uses a water-soluble tomato concentrate, a ginkgo biloba extract and a ginseng extract in combination, and the three work synergistically to effectively relieve fatigue.

[0009] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0010] In the first aspect, the present invention provides a composition for relieving human physical fatigue, comprising: a water-soluble tomato concentrate, a ginkgo biloba extract and a ginseng extract.

[0011] In a preferred embodiment, in the composition for relieving human physical fatigue of the present invention, the mass ratio of the water-soluble tomato concentrate, the ginkgo biloba extract and the ginseng extract is 1-5:1-5:1-10, preferably 2-5:2-5:2-8, more preferably 2-5:2:2-8.

[0012] In one embodiment, in the composition for relieving human physical fatigue of the present invention, the mass ratio of the water-soluble tomato concentrate, the ginkgo biloba extract and the ginseng extract is 5:2:8.

[0013] In one embodiment, in the composition for relieving human physical fatigue of the present invention, the mass ratio of the water-soluble tomato concentrate, the ginkgo biloba extract and the ginseng extract is 2:2:8.

[0014] In the composition for relieving human physical fatigue of the present invention, the water-soluble tomato concentrate is prepared according to the method of the invention patent with the publication number CN114617920B. Preferably, the preparation method of the water-soluble tomato concentrate comprises the following steps:

[0015] Step 1, prepare mixture A of tomato homogenate;

[0016] Step 2: Separate and remove water-insoluble solids with a particle size greater than 500 μm from mixture A to obtain water-soluble component B;

[0017] Step 3: Filter water-soluble component B to obtain filtrate C;

[0018] Step 4: Purify filtrate C to obtain water-soluble tomato active ingredient D;

[0019] Step 5: Concentrate water-soluble tomato active ingredient D to obtain the water-soluble tomato concentrate.

[0020] More preferably, in step 1, the pH of mixture A does not exceed 5.5 and the temperature does not exceed 35°C.

[0021] More preferably, in step 2, the operating temperature of the separation does not exceed 60°C. The separation includes at least one of centrifugal separation and natural sedimentation separation. Water-soluble component B is the supernatant obtained after separation.

[0022] More preferably, in step 3, the filtration is carried out using a filter membrane, and the pore size of the filter membrane is not greater than 1 μm.

[0023] More preferably, the purification in step 4 can adopt alcohol precipitation method or column chromatography method to remove water-soluble saccharides in filtrate C. In one embodiment, the purification in step 4 adopts the alcohol precipitation method, and the alcohol precipitation method is specifically: adding ethanol to filtrate C to make the ethanol concentration in filtrate C higher than 70%, more preferably higher than 90%. In one embodiment, the purification in step 4 adopts column chromatography method, and the column packing of the chromatography column used in the column chromatography method is food-grade resin, preferably Amberlite FPX66 macroporous adsorption resin.

[0024] More preferably, in step 5, the operating temperature of the concentration does not exceed 60°C. The concentration can adopt membrane filtration concentration, low-temperature evaporation concentration, freeze-drying concentration or spray-drying concentration.

[0025] Preferably, in the composition for relieving physical fatigue of the present invention, the water-soluble tomato concentrate includes nucleoside compounds, flavonoid compounds and phenolic acid compounds, and the total content of the nucleoside compounds, flavonoid compounds and phenolic acid compounds in the water-soluble tomato concentrate is ≥14 mg / g.

[0026] In the composition for relieving human physical fatigue according to the present invention, the ginkgo biloba extract can be obtained conventionally and can be a commercially available product, which is not limited herein. In one embodiment, the ginkgo biloba extract meets the requirements of the Chinese Pharmacopoeia (2020 Edition). The ginkgo biloba extract includes total flavonol glycosides and terpene lactones. The total mass percentage of the total flavonol glycosides in the ginkgo biloba extract is ≥24.0%, and the mass percentage of the terpene lactones in the ginkgo biloba extract is ≥6.0%.

[0027] In the composition for relieving human physical fatigue according to the present invention, the ginseng extract can be obtained conventionally and can be a commercially available product, which is not limited herein. In one embodiment, the ginseng extract includes ginsenoside Re, and the mass percentage of ginsenoside Re in the ginseng extract is ≥10%.

[0028] The composition for relieving human physical fatigue according to the present invention includes: water-soluble tomato concentrate, ginkgo biloba extract and ginseng extract. Experimental data show that by using the composition of water-soluble tomato concentrate, ginkgo biloba extract and ginseng extract and compounding the three, a synergistic effect is achieved. It can reduce the content of serum urea and blood lactic acid in mice, increase the reserve of liver glycogen, prolong the weight-bearing swimming time of mice, has the function of relieving physical fatigue, and can be used to regulate sub-health.

[0029] In a second aspect, the present invention also provides an application of a composition for relieving human physical fatigue in the preparation of a product for relieving human physical fatigue. The composition for relieving human physical fatigue is as described in the above technical solution and will not be elaborated herein.

[0030] Preferably, the product for relieving human physical fatigue according to the present invention is a tablet, a capsule, a powder, a granule, a pill or a liquid. In one embodiment, the product for relieving human physical fatigue according to the present invention is a medicine or a health food.

[0031] In one embodiment, the product for relieving human physical fatigue is a tablet, and the product further includes excipients. The excipients preferably include one or more of microcrystalline cellulose, anhydrous lactose, croscarmellose sodium, silicon dioxide and magnesium stearate.

[0032] In one embodiment, the product for relieving human physical fatigue is a capsule, and the product further includes excipients. The excipients preferably include one or more of gelatinized starch, croscarmellose sodium and magnesium stearate.

[0033] The application of the composition for relieving physical fatigue of the human body in the preparation of a product for relieving physical fatigue of the human body, through the combined use of three active ingredients, namely water-soluble tomato concentrate, ginkgo biloba extract and ginseng extract, in the composition for relieving physical fatigue of the human body, can be used in the preparation of a product for relieving physical fatigue of the human body, can synergistically enhance the effect, relieve fatigue, and is used to regulate sub-health.

[0034] In a third aspect, the present invention also provides a product for relieving physical fatigue of the human body, and the product includes: the composition for relieving physical fatigue of the human body according to the above technical solution.

[0035] Preferably, the product for relieving physical fatigue of the human body according to the present invention further includes excipients. The excipients can be any excipients, including but not limited to microcrystalline cellulose, anhydrous lactose, croscarmellose sodium, silicon dioxide, magnesium stearate and gelatinized starch, which are not limited herein. Preferably, the excipients include one or more of microcrystalline cellulose, anhydrous lactose, croscarmellose sodium, silicon dioxide, magnesium stearate and gelatinized starch. The mass ratio of the composition for relieving physical fatigue of the human body to the excipients can be in any proportion, which is not limited herein.

[0036] The product for relieving physical fatigue of the human body according to the present invention, through the combined use of three active ingredients, namely water-soluble tomato concentrate, ginkgo biloba extract and ginseng extract, can synergistically enhance the effect, reduce the contents of serum urea and blood lactic acid in mice, increase the content of liver glycogen, prolong the weight-bearing swimming time of mice, has the function of relieving physical fatigue, and can be used to relieve human fatigue and regulate sub-health.

[0037] Compared with the prior art, the present invention provides a composition for relieving physical fatigue of the human body and its application and product, and has the following beneficial effects:

[0038] (1) The composition for relieving physical fatigue of the human body according to the present invention adopts water-soluble tomato concentrate, ginkgo biloba extract and ginseng extract. The experimental results show that both the complex of ginkgo biloba extract and ginseng extract and water-soluble tomato concentrate have the effects of reducing the contents of serum urea and blood lactic acid in mice, increasing the reserve of liver glycogen, and prolonging the weight-bearing swimming time of mice. After the combined use of water-soluble tomato concentrate, ginkgo biloba extract and ginseng extract, a synergistic effect can be achieved, and it can be used to relieve physical fatigue of the human body.

[0039] (2) By applying the composition for relieving physical fatigue of the human body to a product for relieving physical fatigue of the human body, a product for relieving physical fatigue of the human body is obtained, which can be made into tablets, capsules, powders, granules, pills or liquids, and can be used as a medicine or a health food to regulate human sub-health. Specific embodiments

[0040] It should be noted that, unless otherwise specified, the raw materials used in the present invention are all ordinary commercially available products, and their sources are not specifically limited.

[0041] The following are the sources of raw materials for exemplary illustration:

[0042] Ginkgo biloba extract: Purchased from Guangdong Qingyunshan Pharmaceutical Co., Ltd. According to the COA materials provided by the supplier, the ginkgo biloba extract includes total flavonol glycosides and terpene lactones. The total mass percentage of total flavonol glycosides in the ginkgo biloba extract ≥ 24.0%, and the mass percentage of terpene lactones in the ginkgo biloba extract ≥ 6.0%.

[0043] Ginseng extract: Purchased from Guangdong Qingyunshan Pharmaceutical Co., Ltd. According to the COA materials provided by the supplier, the ginseng extract includes ginsenoside Re, and the mass percentage of ginsenoside Re in the ginseng extract ≥ 10%.

[0044] Example 1

[0045] Preparation of water-soluble tomato concentrate (FF)

[0046] Take ripe tomatoes and homogenize them into a slurry, dilute the slurry with 6 times the weight parts of ultrapure water to obtain mixture A. Add citric acid to mixture A (initial temperature < 30°C) to adjust the pH to 4.0, and centrifuge mixture A at 4°C and 8000×g for 20 min. Take the supernatant (water-soluble component B) and perform ultrafiltration using a ceramic membrane with a pore size of 0.1 μm (working temperature < 60°C), and collect the filtrate C.

[0047] Pack a chromatographic column with 3 times the volume of the filtrate of the food-grade macroporous adsorption resin FPX66 as the filler, and equilibrate the column with ultrapure water. Load the ultrafiltrate onto the column, wash it with 1 times the column volume of distilled water, and then elute it with 1 times the column volume of 80% ethanol, and collect the 80% ethanol eluate (water-soluble active ingredient D).

[0048] Concentrate the eluate by reverse osmosis using a Trisep ACM5 membrane (working temperature < 60°C). The ethanol / water solvent passes through the membrane, while all the compounds dissolved in it are retained inside the membrane. When the volume of the eluate is concentrated to 1 / 10, supplement ultrapure water to the retentate at the same rate of concentration until the supplemented ultrapure water reaches 2 times the volume of the 80% ethanol eluate.

[0049] Collect the retentate, spray-dry it to obtain a fine powder with a water content of less than 6%, which is the water-soluble tomato concentrate. The water-soluble tomato concentrate includes nucleoside compounds, flavonoid compounds, and phenolic acid compounds, and the total content of the nucleoside compounds, flavonoid compounds, and phenolic acid compounds in the water-soluble tomato concentrate ≥ 14 mg / g.

[0050] Example 2

[0051] (1) Experimental conditions and instruments

[0052] The experimental environment was a barrier environment. During the experiment, the temperature was 22°C - 25°C, and the humidity was 46% - 48%. The license number for the use of experimental animals was SYXK (Xiang) 2015 - 0012.

[0053] Main instruments, equipment and reagents: animal weighing balance, electronic balance, centrifuge, water bath, oscillator, 722S spectrophotometer, swimming tank (length × width × height = 53 cm × 40 cm × 35 cm), lead sheet, timer, hemoglobin pipette, pipettor, pipette, test tube.

[0054] (2) Grouping of experimental animals and administration

[0055] Experimental conditions: 320 SPF - level male ICR mice, with a body weight of 18 g - 22 g, provided by Hunan Slack Jingda Experimental Animal Co., Ltd. The production license number for experimental animals was SCXK (Xiang) 2016 - 0002, and the feed was provided by Changsha Tianqin Biotechnology Co., Ltd. The animals were divided into four large groups: Group I, Group II, Group III, and Group IV, with 80 animals in each large group, and the load - bearing swimming experiment, determination of serum urea, blood lactic acid, and liver glycogen were carried out respectively. Each large group was randomly divided into a control group and a test substance group, with 10 mice in each group.

[0056] Sample preparation:

[0057] 1) Sample 1: The water - soluble tomato concentrate prepared in Example 1;

[0058] 2) Sample 2: A composition of ginkgo leaf extract and ginseng extract with a mass ratio of 1:4;

[0059] 3) Sample 3: A composition of water - soluble tomato concentrate, ginkgo leaf extract, and ginseng extract with a mass ratio of 5:2:8;

[0060] 4) Sample 4: A composition of water - soluble tomato concentrate, ginkgo leaf extract, and ginseng extract with a mass ratio of 1:1:4.

[0061] Administration: Different test sample groups were set up, including the low - dose group (10 mg / kg) and high - dose group (25 mg / kg) of Sample 1, the low - dose group (20 mg / kg) and high - dose group (125 mg / kg) of Sample 2, the Sample 3 group (30 mg / kg), and the Sample 4 group (150 mg / kg).

[0062] The test sample was orally administered to mice once a day by gavage, and the gavage volume was 20 mL / kg.bw. The blank control group was gavaged with an equal volume of deionized water instead of the test sample solution. After continuous administration for 30 days, it was used to measure various indicators of the function of relieving physical fatigue.

[0063] (3) Data processing: SPSS software was used to statistically analyze various indicators. First, a homogeneity of variance test was performed on the data. If the variances were homogeneous, one-way ANOVA was used for overall comparison. If there were overall differences, the Dunnett method was used for pairwise comparison between the means of multiple dose groups and one control group. If the variances were not homogeneous, appropriate variable transformations were performed on the original data. After meeting the homogeneity of variance test, the transformed data was used for statistics. If the variances still did not reach the homogeneous purpose after variable transformation, the rank sum test was used for statistics. If there were overall differences in the comparison, the Tamhanes T2 test that did not require homogeneity of variance was used for pairwise comparison.

[0064] Example 3

[0065] Load-bearing swimming test

[0066] 30 minutes after the last administration of the test sample for mice in Example 2, the mice were placed in a swimming tank to swim. The water depth was about 30 cm, the water temperature was 25.0 ± 1.0 °C, and a lead sheet with a load of 5% of the body weight was attached to the root of the mouse tail. The time from the start of swimming to death of the mouse was recorded as the load-bearing swimming time (min). The results are shown in Table 1, which shows the effect of the sample on the load-bearing swimming time of mice.

[0067] Table 1 Effect of the sample on the load-bearing swimming time of mice

[0068]

[0069]

[0070] Note: Compared with the blank control group, *P < 0.05; **P < 0.01

[0071] As can be seen from Table 1, compared with the blank control group, the load-bearing swimming time of mice in the 6 sample groups was all increased. Moreover, the load-bearing swimming time of mice in sample group 3 and sample group 4 was extended by 13.37 min and 17.23 min respectively, and the load-bearing swimming time of mice was significantly extended (P < 0.05 or P < 0.01).

[0072] Compared with the blank control group, the extended time of the weight-bearing swimming time of each group of mice was compared. The extended time of the sample 3 group (13.37 min) increased by 103.19% compared with the sum of the extended times of the low-dose group of sample 1 and the low-dose group of sample 2 (2.76 + 3.82 = 6.58 min). The extended time of the sample 4 group (17.23 min) increased by 29.84% compared with the sum of the extended times of the high-dose group of sample 1 and the high-dose group of sample 2 (7.61 + 5.66 = 13.27 min).

[0073] The results showed that in the compositions of water-soluble tomato concentrate, ginkgo biloba extract and ginseng extract at low and high doses, the three could synergistically enhance the effect, extend the weight-bearing swimming time of mice, improve the exercise durability of the tested mice, and play a role in relieving fatigue.

[0074] Example 4

[0075] Serum urea content determination test

[0076] 30 minutes after the last administration of the mouse test sample in Example 2, the mice swam without load in water at 30 °C for 90 minutes, and then blood was collected by eye socket after resting for 60 minutes. After standing at 4 °C for 3 h, it was centrifuged at 2000 rpm / min for 15 min, and the serum was taken. The serum urea was measured using a serum urea kit (Nanjing Jiancheng Bioengineering Institute) and a fully automatic biochemical analyzer BECKMAN COULTER AU680. The results are shown in Table 2, which shows the effect of the sample on the serum urea content of mice.

[0077] Table 2 Effect of the sample on the serum urea content of mice

[0078]

[0079]

[0080] Note: Compared with the blank control group, *P < 0.05; **P < 0.01

[0081] As can be seen from Table 2, compared with the blank control group, the serum urea content of mice in the 6 sample groups decreased, and the serum urea content of mice in the high-dose group of sample 1, the high-dose group of sample 2, the sample 3 group and the sample 4 group decreased by 2.07 mmol / L, 1.96 mmol / L, 2.12 mmol / L and 2.91 mmol / L respectively, and the serum urea content of mice decreased significantly (P < 0.05 or P < 0.01).

[0082] Compared with the blank control group, the decreased content of serum urea in each group of mice was compared. The decreased content of the sample 3 group (2.12 mmol / L) was 48% higher than the sum of the decreased contents of the low-dose groups of sample 1 and sample 2 (0.34 + 1.09 = 1.43 mmol / L). The decreased content of the sample 4 group of mice (2.91 mmol / L) was 28% lower than the sum of the decreased contents of the high-dose groups of sample 1 and sample 2 (2.07 + 1.96 = 4.03 mmol / L).

[0083] Serum urea is a product of protein metabolism. In the case of exhaustive exercise, the body's energy metabolism balance is disrupted, glycogen is consumed excessively and the supply is insufficient, the catabolism rate of proteins and amino acids increases significantly, the catabolism of structural proteins and functional proteins in the body intensifies, and the kidney function declines, resulting in a decrease in the ability to clear urea, leading to an increase in serum urea content.

[0084] The results showed that in the composition of low-dose water-soluble tomato concentrate, ginkgo biloba extract and ginseng extract, the three can synergistically enhance the effect, reduce the serum urea content in mice, relieve the accumulation of blood urea caused by exercise in the test mice, improve the ability to clear urea, and thus play a role in relieving fatigue.

[0085] Example 5

[0086] Determination of liver glycogen:

[0087] Using the anthrone method, 30 minutes after the last administration of the test sample to the mice in Example 2, the animals were sacrificed, the liver was taken, rinsed with physiological saline, blotted dry with filter paper, and 100 mg of the liver was weighed. The liver glycogen was determined according to the instructions of the liver glycogen test kit (Nanjing Jiancheng Bioengineering Institute).

[0088] Determination of the area under the blood lactate curve:

[0089] 30 minutes after the last administration of the test sample to the mice in Example 2, 20 μL of blood was collected from the internal vein of the mice. After that, the mice swam in water at 30°C without load for 10 minutes and then stopped. 20 μL of blood was collected from the internal vein of the mice at 0 minute and 20 minutes after exercise respectively. A Lactate-Scout lactate analyzer was used to measure the blood lactate, and the area under the blood lactate curve was calculated. The calculation formula is as follows:

[0090] Area under the blood lactate curve = 1 / 2 X (blood lactate value before swimming + blood lactate value at 0 minute after swimming) X 10 + 1 / 2 X (blood lactate value at 0 minute after swimming + blood lactate value after resting for 20 minutes after swimming) X 20

[0091] The results are shown in Table 3, and Table 3 shows the effects of the samples on liver glycogen and the area under the blood lactate curve in mice.

[0092] Table 3 Effects of Samples on the Areas under the Curves of Hepatic Glycogen and Blood Lactate in Mice

[0093]

[0094] Note: Compared with the blank control group, *P<0.05; **P<0.01

[0095] As can be seen from Table 3, compared with the blank control group, the hepatic glycogen content in mice of the 6 sample groups all increased to some extent, and the areas under the curves of blood lactate all decreased to some extent. Moreover, the hepatic glycogen content in mice of sample group 4 increased significantly (P<0.05), by 6.322 mg / g, and the area under the curve of blood lactate decreased significantly (P<0.01), by 46.59.

[0096] Compared with the blank control group, when comparing the increased amounts of hepatic glycogen content in mice of each group, the increased amount in sample group 3 (3.551 mg / g) was 100% higher than the total increased amounts in the low-dose group of sample 1 and the low-dose group of sample 2 (1.334 + 0.44 = 1.774 mmol / L); the increased amount in sample group 4 (6.322 mmol / L) was 16% higher than the total increased amounts in the high-dose group of sample 1 and the high-dose group of sample 2 (3.361 + 2.103 = 5.464 mmol / L), with little difference.

[0097] Compared with the blank control group, when comparing the decreased values of the areas under the curves of blood lactate in mice of each group, the decreased value in sample group 3 (25.26) was 112% higher than the total decreased values in the low-dose group of sample 1 and the low-dose group of sample 2 (6.35 + 5.55 = 11.9) of hepatic glycogen content in mice, and the decreased value in sample group 4 (46.59) was 30% higher than the total decreased values in the high-dose group of sample 1 and the high-dose group of sample 2 (19.35 + 16.5 = 35.85).

[0098] After exhaustive exercise, it will lead to a large accumulation of lactic acid and a rapid consumption of body glycogen. Hepatic glycogen is used to break down and release glucose to maintain blood glucose concentration. A decrease in blood glucose concentration will cause disorders in the function of the central nervous system and body fatigue.

[0099] The results show that in the compositions of water-soluble tomato concentrate, ginkgo biloba extract and ginseng extract at low and high doses, the three can synergistically enhance the effect, increase the hepatic glycogen content in mice, promote the hepatic glycogen reserve in mice, reduce the area under the curve of blood lactate, and reduce and relieve the lactic acid accumulation caused by exercise in the test mice, thus playing a role in relieving fatigue.

[0100] Based on the analysis of the test results in Examples 3 - 5, if the result of the load-bearing swimming test is positive and any two of the three biochemical indexes of blood lactic acid, serum urea, and liver glycogen are positive, it can be determined that the test substance has the function of relieving physical fatigue. In summary, the water-soluble tomato concentrate, ginkgo biloba extract, and ginseng extract composition can reduce the contents of serum urea and blood lactic acid, increase the reserve of liver glycogen, prolong the load-bearing swimming time of mice, relieve physical fatigue, and can be used to regulate sub-health.

[0101] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the protection scope of the present invention. Any simple modification or equivalent replacement made by those of ordinary skill in the art to the technical solution of the present invention shall not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A composition for relieving human physical fatigue, characterized in that: include: Water-soluble tomato concentrate, ginkgo biloba extract and ginseng extract.

2. The composition for relieving human physical fatigue according to claim 1, characterized in that: The mass ratio of the water-soluble tomato concentrate, the ginkgo leaf extract and the ginseng extract is 1-5:1-5:1-10.

3. The composition for relieving human physical fatigue according to claim 2, characterized in that: The mass ratio of the water-soluble tomato concentrate, the ginkgo leaf extract and the ginseng extract is 2-5:2-5:2-8; preferably 2-5:2:

8.

4. The composition for relieving human physical fatigue according to any one of claims 1 to 3, characterized in that: The preparation method of the water-soluble tomato concentrate comprises the following steps: Step 1, preparing a mixture A of tomato homogenate; Step 2, separating and removing water-insoluble solids with a particle size greater than 500 μm from mixture A to obtain a water-soluble component B; Step 3, filtering the water-soluble component B to obtain a filtrate C; Step 4, purifying the filtrate C to obtain the tomato water-soluble active ingredient D; Step 5, concentrating the tomato water-soluble active ingredient D to obtain the water-soluble tomato concentrate.

5. The composition for relieving human physical fatigue according to claim 4, characterized in that: In the step 2, the operating temperature of the separation is not higher than 60° C., the separation includes at least one of centrifugal separation and natural sedimentation separation, and the water-soluble component B is the supernatant obtained after separation.

6. Use of the composition for relieving human physical fatigue according to any one of claims 1 to 5 in the preparation of a product for relieving human physical fatigue.

7. The use according to claim 6, characterized in that: The product is in the form of tablets, capsules, powders, granules, pills or liquids.

8. The use according to claim 6 or 7, characterized in that: The product is a medicine or a health food.

9. A product for relieving human physical fatigue, characterized in that: The product comprises: the composition for relieving human physical fatigue as described in any one of claims 1 to 5.

10. The product for relieving human physical fatigue according to claim 9, characterized in that: The product for relieving human physical fatigue also includes auxiliary materials, which include one or more of microcrystalline cellulose, anhydrous lactose, cross-linked sodium carboxymethyl cellulose, silicon dioxide, magnesium stearate and gelatinized starch.

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