A high-speed single-rotation countercurrent chromatograph

Abstract

The present invention discloses a high-speed single-rotation countercurrent chromatograph, which relates to the field of countercurrent chromatographs. It includes a liquid inlet chamber rotatably arranged, the liquid inlet chamber is coaxially connected with a liquid changing chamber, an injection mechanism is arranged in the liquid inlet chamber, a spiral tube mounting base plate is arranged in the liquid changing chamber, a spiral tube is arranged on the spiral tube mounting base plate, a liquid inlet is arranged on the liquid inlet chamber, the liquid inlet is connected to the injection port of the injection mechanism through a one-way liquid inlet pipeline, and the injection port is connected to the liquid inlet end of the spiral tube through a one-way liquid outlet pipeline. Adopting a single-rotation structure and canceling the setting of the rotary joint at the same time, there is no need to worry about the aging and leakage of the joint seal due to too high rotation speed, and the improvement of the rotation speed is not limited by the maximum working rotation speed of the rotary joint, so a higher rotation speed can be achieved. Secondly, the sample is pre-set inside the pipeline system, and the sample does not need to flow through the rotary joint from the outside of the pipeline system and then be injected into the internal pipeline, avoiding the too high temperature at the rotary joint when the rotary joint rotates at a high speed, which may damage the activity of the sample and the stability of the solvent system.

Description

Technical Field

[0001] The present invention relates to the field of countercurrent chromatographs, and particularly to a high-speed single-rotation countercurrent chromatograph. Background Art

[0002] Countercurrent chromatography technology is a continuous and efficient liquid-liquid partition chromatography separation technology developed in the 1970s of the 20th century. It generates a two-dimensional force field through the synchronous planetary motion of simultaneous revolution and rotation, retains one of the two phases as the stationary phase, and realizes separation and extraction during high-speed rotation. For traditional J-type chromatographs, the pipeline needs to be unwound. Therefore, a planetary gear train is used as the power transmission, and the pipeline unwinding is achieved through the cooperation of revolution and rotation. However, the planetary gear train has disadvantages such as poor dynamic balance, poor performance, high noise, low safety, and being unfavorable for miniaturization. Therefore, currently, countercurrent chromatography begins to develop in the direction of single rotation. The rotation speed of the single-rotation countercurrent chromatograph has been increased compared with that of the J-type chromatograph. However, the rotation speed of the single-rotation countercurrent chromatograph is limited by the maximum working rotation speed of the rotary joint, resulting in the inability to obtain a higher rotation speed. Secondly, when the rotation speed of the rotary joint is too high, the temperature at the rotary joint is too high, which will damage the activity of the sample and the stability of the solvent system. Therefore, the structure of the single-rotation countercurrent chromatograph needs to be further optimized. Summary of the Invention

[0003] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a high-speed single-rotation countercurrent chromatograph to solve the deficiencies of the prior art.

[0004] The purpose of the present invention is achieved through the following technical solutions: A high-speed single-rotation countercurrent chromatograph includes a rotatably arranged liquid inlet chamber, the liquid inlet chamber is coaxially connected with a liquid changing chamber, an injection mechanism is arranged in the liquid inlet chamber, a spiral tube mounting base plate is arranged in the liquid changing chamber, a spiral tube is arranged on the spiral tube mounting base plate, a liquid inlet is arranged on the liquid inlet chamber, the liquid inlet is connected to the injection port of the injection mechanism through a one-way liquid inlet pipeline, and the injection port is connected to the liquid inlet end of the spiral tube through a one-way liquid outlet pipeline.

[0005] Further, the one-way liquid inlet pipeline includes a left-through one-way valve, a first liquid inlet pipe, and a second liquid inlet pipe. The two ends of the second liquid inlet pipe are respectively connected to the liquid inlet and the right end of the left-through one-way valve, and the two ends of the first liquid inlet pipe are respectively connected to the injection port and the left end of the left-through one-way valve.

[0006] Further, one end of the liquid changing chamber away from the liquid inlet chamber is coaxially connected with a liquid storage chamber. A liquid collection box and a waste liquid box are arranged in the liquid storage chamber. The one-way liquid outlet pipeline includes a first right-through one-way valve, a first liquid outlet pipe, and a second liquid outlet pipe. Two ends of the first liquid outlet pipe are respectively connected to the injection port and the left end of the first right-through one-way valve. Two ends of the second liquid outlet pipe are respectively connected to the right end of the first right-through one-way valve and the liquid changing assembly. The liquid changing assembly is used to introduce the liquid discharged from the spiral tube into the liquid collection box or the waste liquid box.

[0007] Further, the liquid changing assembly includes a liquid inlet reversing valve, a liquid outlet reversing valve, a sample box, and a second right-through one-way valve. The second liquid outlet pipe is connected to the liquid inlet of the liquid inlet reversing valve. The second liquid outlet of the liquid inlet reversing valve is connected to the liquid inlet end of the spiral tube through a seventh pipeline. The first liquid outlet of the liquid inlet reversing valve is connected to the sample box through a first pipeline. The sample box is connected to the left end of the second right-through one-way valve through a second pipeline. The right end of the second right-through one-way valve is connected to the liquid inlet end of the spiral tube through a third pipeline. The liquid outlet end of the spiral tube is connected to the liquid inlet of the liquid outlet reversing valve through a fourth pipeline. The first liquid outlet of the liquid outlet reversing valve is connected to the liquid collection box through a fifth pipeline. The second liquid outlet of the liquid outlet reversing valve is connected to the waste liquid box through a sixth pipeline.

[0008] Further, the injection mechanism includes a syringe and an injection pump motor. The output shaft of the injection pump motor is connected to the piston shaft of the syringe. A syringe mounting flange is sleeved on the syringe. The syringe mounting flange is installed at one end of the liquid inlet chamber away from the liquid changing chamber. The injection pump motor is installed on a motor mounting flange. The motor mounting flange and the syringe mounting flange are connected through a bearing mounting ring.

[0009] Further, it further includes an equipment bottom plate. A left bearing seat and a right bearing seat are fixed on the equipment bottom plate. One end of the liquid storage chamber away from the liquid changing chamber is coaxially connected with a right connecting shaft. One end of the bearing mounting ring away from the liquid inlet chamber is coaxially connected with a left connecting shaft. The left connecting shaft and the right connecting shaft are respectively rotatably installed on the left bearing seat and the right bearing seat.

[0010] Further, a driving motor is installed on the equipment bottom plate. The output shaft of the driving motor is connected with a driving pulley. A driven pulley is sleeved on the liquid inlet chamber. The driven pulley is connected to the driving pulley through a synchronous belt for transmission.

[0011] Further, a slip ring is sleeved on the left connecting shaft. The slip ring is used for wiring the injection pump motor, the liquid inlet reversing valve, and the liquid outlet reversing valve.

[0012] Furthermore, a support bearing seat is fixed on the equipment base plate, and the bearing installation ring is rotatably installed on the support bearing seat through a support bearing.

[0013] Furthermore, the end face of the spiral tube installation base plate where the spiral tube is arranged is connected with a spiral tube installation cover plate through screws. The spiral tube is restricted between the spiral tube installation base plate and the spiral tube installation cover plate. Central holes are provided on both the spiral tube installation cover plate and the spiral tube installation base plate.

[0014] The beneficial effects of the present invention are as follows:

[0015] 1. The single self-rotation structure is adopted, but the setting of the rotary joint is cancelled. Therefore, there is no need to worry about the aging leakage of the joint seal at too high a rotation speed. The increase in rotation speed is not limited by the maximum operating rotation speed of the rotary joint, and higher rotation speeds can be achieved, with better separation effects and separation efficiency.

[0016] 2. The sample is pre-set inside the pipeline system and rotates together with the entire pipeline. When it is necessary to switch to the injection loop for injection midway, the sample no longer has to flow through the rotary joint and then be injected into the internal spiral pipeline from the outside of the pipeline system, preventing the temperature at the rotary joint from being too high when the rotary joint rotates at too high a speed, which may damage the activity of the sample and the stability of the solvent system. Therefore, the spiral tube can maintain a higher rotation speed without worrying about the influence of the temperature rise of the rotary joint on injection in the traditional single self-rotation countercurrent chromatograph.

[0017] 3. All mechanisms are arranged symmetrically in the center to ensure dynamic balance at high rotation speeds of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a schematic structural diagram of a high-rotation-speed single self-rotation countercurrent chromatograph of the present invention Figure 1 ;

[0019] Figure 2 is a top view of a high-rotation-speed single self-rotation countercurrent chromatograph of the present invention;

[0020] Figure 3 is Figure 2 a cross-sectional view taken along the A-A direction in

[0021] Figure 4 is Figure 3 an enlarged view at A in

[0022] Figure 5 is a schematic structural diagram of a high-rotation-speed single self-rotation countercurrent chromatograph of the present invention Figure 2 ;

[0023] In the figure, 1 is the liquid inlet chamber, 2 is the spiral tube mounting base plate, 3 is the spiral tube, 4 is the liquid inlet, 5 is the left-through one-way valve, 6 is the first liquid inlet pipe, 7 is the second liquid inlet pipe, 8 is the first right-through one-way valve, 9 is the liquid collection box, 10 is the waste liquid box, 11 is the first liquid outlet pipe, 12 is the second liquid outlet pipe, 13 is the liquid inlet reversing valve, 14 is the liquid outlet reversing valve, 15 is the sample box, 16 is the second right-through one-way valve, 17 is the first pipeline, 18 is the second pipeline, 19 is the third pipeline, 20 is the fourth pipeline, 21 is the fifth pipeline, 22 is the sixth pipeline, 23 is the syringe, 24 is the syringe pump motor, 25 is the syringe mounting flange, 26 is the liquid change chamber, 27 is the liquid storage chamber, 28 is the motor mounting flange, 29 is the bearing mounting ring, 30 is the equipment base plate, 31 is the left bearing seat, 32 is the right bearing seat, 33 is the right connecting shaft, 34 is the left connecting shaft, 35 is the driving motor, 36 is the driving pulley, 37 is the driven pulley, 38 is the synchronous belt, 39 is the electric slip ring, 40 is the support bearing seat, 41 is the support bearing, 42 is the spiral tube mounting cover plate, 43 is the seventh pipeline, 44 is the encoder. Detailed implementation mode

[0024] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings, but the protection scope of the present invention is not limited to the following description.

[0025] Embodiment 1

[0026] As Figures 1 to 5 shown, a high-speed single-rotation countercurrent chromatograph includes a rotatably arranged liquid inlet chamber 1, the liquid inlet chamber 1 is coaxially connected with a liquid change chamber 26, an injection mechanism is arranged in the liquid inlet chamber 1, a spiral tube mounting base plate 2 is arranged in the liquid change chamber 26, a spiral tube 3 is arranged on the spiral tube mounting base plate 2, a liquid inlet 4 is arranged on the liquid inlet chamber 1, the liquid inlet 4 is connected to the injection port of the injection mechanism through a one-way liquid inlet pipeline, the injection port is connected to the liquid inlet end of the spiral tube 3 through a one-way liquid outlet pipeline, the main structure formed by the liquid inlet chamber 1 and the liquid change chamber 26 is directly connected to a driving device, the arrangement of the planetary gear train is cancelled, a single-rotation structure countercurrent chromatograph is formed, the sample is preset in the injection mechanism, so that the sample does not need to enter the countercurrent chromatograph from the outside through a rotary joint, and directly enters the spiral tube 3 through the injection mechanism and the one-way liquid outlet pipeline. During this process, the main structure rotates at a high speed to achieve separation and extraction, thereby cancelling the setting of the rotary joint, without worrying about the aging and leakage of the joint seal at too high a speed, the increase in speed is not limited by the maximum working speed of the rotary joint, a higher speed can be achieved, and better separation effect and separation efficiency can be obtained.

[0027] Furthermore, on the end face of the spiral tube installation base plate 2 where the spiral tube 3 is arranged, a spiral tube installation cover plate 42 is connected by screws. The spiral tube 3 is restricted between the spiral tube installation base plate 2 and the spiral tube installation cover plate 42. Central holes are provided on both the spiral tube installation cover plate 42 and the spiral tube installation base plate 2. Circular grooves are provided on the end face of the spiral tube installation base plate 2 close to the spiral tube 3 and the end face of the spiral tube installation cover plate 42 close to the spiral tube 3. The spiral tube 3 is arranged in the space formed by the two circular grooves, and the spiral tube 3 is spirally coiled in this space. An inlet liquid round hole communicating with the circular groove is provided on the end face of the spiral tube installation base plate 2 far from the spiral tube 3, and an outlet liquid round hole communicating with the circular groove is provided on the end face of the spiral tube installation cover plate 42 far from the spiral tube 3. The inlet end of the spiral tube 3 extends out from the inlet liquid round hole, and the outlet end extends out from the outlet liquid round hole.

[0028] Embodiment Two

[0029] On the basis of Embodiment One, as Figures 1 to 4 shown, the one-way inlet liquid pipeline includes a left-through one-way valve 5, a first inlet liquid pipe 6 and a second inlet liquid pipe 7. The two ends of the second inlet liquid pipe 7 are respectively connected to the inlet port 4 and the right end of the left-through one-way valve 5. The two ends of the first inlet liquid pipe 6 are respectively connected to the injection port and the left end of the left-through one-way valve 5. The injection mechanism includes a syringe 23 and an injection pump motor 24. The output shaft of the injection pump motor 24 is connected to the piston shaft of the syringe 23. A syringe installation flange 25 is sleeved on the syringe 23. The syringe installation flange 25 is installed at one end of the liquid inlet chamber 1 far from the liquid changing chamber 26. The injection pump motor 24 is installed on the motor installation flange 28. The motor installation flange 28 and the syringe installation flange 25 are connected through a bearing installation ring 29. Connect an external pipeline to the inlet port 4, drive the piston of the syringe 23 to move through the injection pump motor 24, so that the sample passes through the external pipeline, the inlet port 4, the second inlet liquid pipe 7, the left-through one-way valve 5, and the first inlet liquid pipe 6 and enters the syringe 23 to preset the sample in the syringe 23. Then disconnect the external pipeline, and then the injection pump motor 24 injects the sample into the spiral tube 3 through the one-way outlet liquid pipeline. Under the one-way conduction of the left-through one-way valve 5, the sample can stably enter the spiral tube 3 through the one-way outlet liquid pipeline.

[0030] Embodiment Three

[0031] Since two-phase solutions need to be preset for extraction and separation, in order to enable the two-phase solutions to be smoothly injected into the spiral tube, on the basis of Embodiment Two, as Figures 1 to 4As shown, a liquid storage chamber 27 is coaxially connected to one end of the liquid changing chamber 26 away from the liquid inlet chamber 1. A liquid collecting box 9 and a waste liquid box 10 are arranged in the liquid storage chamber 27. The one-way liquid outlet pipeline includes a first right-pass one-way valve 8, a first liquid outlet pipe 11 and a second liquid outlet pipe 12. The two ends of the first liquid outlet pipe 11 are respectively connected to the injection port and the left end of the first right-pass one-way valve 8. The two ends of the second liquid outlet pipe 12 are respectively connected to the right end of the first right-pass one-way valve 8 and the liquid changing assembly. The liquid changing assembly is used to introduce the liquid discharged from the spiral tube 3 into the liquid collecting box 9 or the waste liquid box 10. The liquid changing assembly includes a liquid inlet reversing valve 13, a liquid outlet reversing valve 14, a sample box 15 and a second right-pass one-way valve 16. The sample box 15 pre-stores the extraction agent. The second liquid outlet pipe 12 is connected to the liquid inlet of the liquid inlet reversing valve 13. The second liquid outlet of the liquid inlet reversing valve 13 is connected to the liquid inlet end of the spiral tube 3 through a seventh pipeline 43. The first liquid outlet of the liquid inlet reversing valve 13 is connected to the sample box 15 through a first pipeline 17. The sample box 15 is connected to the left end of the second right-pass one-way valve 16 through a second pipeline 18. The right end of the second right-pass one-way valve 16 is connected to the liquid inlet end of the spiral tube 3 through a third pipeline 19. The liquid outlet end of the spiral tube 3 is connected to the liquid inlet of the liquid outlet reversing valve 14 through a fourth pipeline 20. The first liquid outlet of the liquid outlet reversing valve 14 is connected to the liquid collecting box 9 through a fifth pipeline 21. The second liquid outlet of the liquid outlet reversing valve 14 is connected to the waste liquid box 10 through a sixth pipeline 22. Connect the liquid inlet to the external pipeline and start the injection mechanism. First, pump the A solution into the syringe 23. By adjusting the conduction path of the liquid inlet reversing valve 13 and the liquid outlet reversing valve 14, control the flow path of the A solvent. First, make the waste liquid in the initial stage of extraction enter the waste liquid box 10, and then make the liquid after stable extraction separation enter the liquid collecting box 9. The specific extraction separation process is as follows: The A solution in the syringe 23 enters the spiral tube 3 from the first liquid outlet pipe 11, the first right-pass one-way valve 8, the second liquid outlet pipe 12, the liquid inlet reversing valve 13, and the seventh pipeline 43, and then enters the waste liquid box 10 from the liquid outlet end of the spiral tube 3, the fourth pipeline 20, the liquid outlet reversing valve 14, and the sixth pipeline 22. The syringe 23 repeatedly pumps and pumps out the A solution. When the solution flows into the waste liquid box 10, it indicates that the A solution fills the entire one-way liquid outlet pipeline. Then pump the B solution. The injection pump motor 24 pumps the B solution into the syringe 23 and pre-sets the B solution in the syringe 23. Then disconnect the external pipeline, and the main body structure rotates at a high speed for extraction operation. The syringe 23 gradually pumps the B solution into the one-way liquid outlet pipeline. First, make the one-way liquid outlet pipeline discharge the solution into the waste liquid box 10. After stable extraction separation, then discharge the solution into the liquid collecting box 9. Specifically, the B solution enters the sample box 15 from the first liquid outlet pipe 11, the first right-pass one-way valve 8, the second liquid outlet pipe 12, the liquid inlet reversing valve 13, and the first pipeline 17, thereby pushing out the sample in the sample box 15, and making the B solution carry the sample liquid through the second pipeline 18, the second right-pass one-way valve 16, and the third pipeline 19 into the spiral tube 3, so that the spiral tube 3 contains the A solution, the B solution and the sample liquid.The main structure drives the spiral tube 3 to rotate at a high speed to complete the extraction and separation process. In order to ensure that the stably separated solution enters the liquid collecting box 9, in the initial process of extraction, the initial waste liquid of the extraction is first discharged into the waste liquid box 10, that is, the solution flowing out of the spiral tube 3 enters the waste liquid box 10 through the fourth pipeline 20, the liquid outlet reversing valve 14, and the sixth pipeline 22. After a period of time, the outflow path of the solution is switched so that the solution flowing out of the spiral tube 3 enters the liquid collecting box through the fourth pipeline 20, the liquid outlet reversing valve 14, and the fifth pipeline 21. 9, so that the extracted and separated solution enters the liquid collection box 9 for storage; in specific implementation, the liquid inlet tank 1, the liquid exchange tank 26 and the liquid storage tank 27 are connected in sequence by flanges, which is convenient for separate disassembly, and the liquid inlet tank 1, the liquid exchange tank 26 and the liquid storage tank 27 all adopt a half-type structure, that is, two semi-cylindrical barrels are connected into a cylindrical barrel by screws, which is convenient for disassembling the liquid inlet tank 1 in half to arrange the injection mechanism, disassembling the liquid exchange tank 26 to arrange the one-way liquid outlet pipeline, and disassembling the liquid storage tank 27 in half to take out or put into the liquid collection box 9 and the waste liquid box 10.

[0032] Embodiment 4

[0033] Based on the third embodiment, Figures 1 to 5 As shown, the device also includes a bottom plate 30, on which a left bearing seat 31 and a right bearing seat 32 are fixed, one end of the liquid storage tank 27 away from the liquid exchange tank 26 is coaxially connected to a right connecting shaft 33, and one end of the bearing mounting ring 29 away from the liquid inlet tank 1 is coaxially connected to a left connecting shaft 34, and the left connecting shaft 34 and the right connecting shaft 33 are rotatably mounted on the left bearing seat 31 and the right bearing seat 32 respectively, a driving motor 35 is installed on the bottom plate 30, and the output shaft of the driving motor 35 is connected to a driving pulley 36, and a The driving pulley 37 is connected to the active pulley 36 through the synchronous belt 38. The driving pulley 37 drives the active pulley 36 to rotate. The active pulley 36 drives the driving pulley 37 to rotate through the synchronous belt 38. The driving pulley 37 drives the entire main structure to rotate at a high speed to form a single-rotation countercurrent chromatograph. A support bearing seat 40 is fixed on the bottom plate 30 of the equipment. The bearing mounting ring 29 is rotatably mounted on the support bearing seat 40 through the support bearing 41. All mechanisms are arranged symmetrically in the center to ensure dynamic balance of the equipment at high speed.

[0034] Furthermore, an electric slip ring 39 is mounted on the left connecting shaft 34, and the electric slip ring 39 is used to wire the injection pump motor 24, the liquid inlet reversing valve 13 and the liquid outlet reversing valve 14, and to unwind the wires of the injection pump motor 24, the liquid inlet reversing valve 13 and the liquid outlet reversing valve 14. An encoder 44 is provided at the end of the left connecting shaft 34 away from the injection mechanism.

Claims

1. A high-speed single-rotation countercurrent chromatograph, characterized in that, It includes a liquid inlet chamber (1) which is rotatably arranged. The liquid inlet chamber (1) is coaxially connected with a liquid changing chamber (26). One end of the liquid changing chamber (26) away from the liquid inlet chamber (1) is coaxially connected with a liquid storage chamber (27). A liquid collecting box (9) and a waste liquid box (10) are arranged in the liquid storage chamber (27). An injection mechanism is arranged in the liquid inlet chamber (1). A spiral tube mounting base plate (2) is arranged in the liquid changing chamber (26). A spiral tube (3) is arranged on the spiral tube mounting base plate (2). A liquid inlet (4) is arranged on the liquid inlet chamber (1). The liquid inlet (4) is connected to the injection port of the injection mechanism through a one-way liquid inlet pipeline. The injection port is connected to a liquid changing component through a one-way liquid outlet pipeline. The liquid changing component includes a liquid inlet reversing valve (13), a liquid outlet reversing valve (14), a sample box (15) and a second right-through one-way valve (16). The liquid inlet of the liquid inlet reversing valve (13) is connected to the one-way liquid outlet pipeline. The second liquid outlet of the liquid inlet reversing valve (13) is connected to the liquid inlet end of the spiral tube (3) through a seventh pipeline (43). The first liquid outlet of the liquid inlet reversing valve (13) is connected to the sample box (15) through a first pipeline (17). The sample box (15) is connected to the left end of the second right-through one-way valve (16) through a second pipeline (18). The right end of the second right-through one-way valve (16) is connected to the liquid inlet end of the spiral tube (3) through a third pipeline (19). The liquid outlet end of the spiral tube (3) is connected to the liquid inlet of the liquid outlet reversing valve (14) through a fourth pipeline (20). The first liquid outlet of the liquid outlet reversing valve (14) is connected to the liquid collecting box (9) through a fifth pipeline (21). The second liquid outlet of the liquid outlet reversing valve (14) is connected to the waste liquid box (10) through a sixth pipeline (22).

2. The high-speed single-rotation countercurrent chromatograph according to claim 1, characterized in that, The one-way liquid inlet pipeline includes a left-through one-way valve (5), a first liquid inlet pipe (6) and a second liquid inlet pipe (7). Two ends of the second liquid inlet pipe (7) are respectively connected to the liquid inlet (4) and the right end of the left-through one-way valve (5). Two ends of the first liquid inlet pipe (6) are respectively connected to the injection port and the left end of the left-through one-way valve (5).

3. A high-speed single-rotation countercurrent chromatograph according to claim 1, characterized in that, The one-way liquid outlet pipeline includes a first right-through one-way valve (8), a first liquid outlet pipe (11) and a second liquid outlet pipe (12). Two ends of the first liquid outlet pipe (11) are respectively connected to the injection port and the left end of the first right-through one-way valve (8). Two ends of the second liquid outlet pipe (12) are respectively connected to the right end of the first right-through one-way valve (8) and the liquid changing component.

4. The high-speed single-rotation countercurrent chromatograph according to claim 1, wherein The injection mechanism includes a syringe (23) and an injection pump motor (24). The output shaft of the injection pump motor (24) is connected to the piston shaft of the syringe (23). A syringe mounting flange (25) is sleeved on the syringe (23). The syringe mounting flange (25) is installed at one end of the liquid inlet chamber (1) away from the liquid changing chamber (26). The injection pump motor (24) is installed on a motor mounting flange (28). The motor mounting flange (28) and the syringe mounting flange (25) are connected through a bearing mounting ring (29).

5. The high-speed single-rotation countercurrent chromatograph according to claim 4, characterized in that, It further includes a device bottom plate (30), on which a left bearing seat (31) and a right bearing seat (32) are fixed. One end of the liquid storage chamber (27) far from the liquid changing chamber (26) is coaxially connected with a right connecting shaft (33), and one end of the bearing mounting ring (29) far from the liquid inlet chamber (1) is coaxially connected with a left connecting shaft (34). The left connecting shaft (34) and the right connecting shaft (33) are respectively rotatably mounted on the left bearing seat (31) and the right bearing seat (32).

6. A high-speed single-rotation countercurrent chromatograph according to claim 5, characterized in that, A driving motor (35) is mounted on the device bottom plate (30). The output shaft of the driving motor (35) is connected with a driving pulley (36). A driving pulley (37) is sleeved on the liquid inlet chamber (1). The driving pulley (37) is in transmission connection with the driving pulley (36) through a timing belt (38).

7. A high-speed single-rotation countercurrent chromatograph according to claim 5, characterized in that, A slip ring (39) is sleeved on the left connecting shaft (34). The slip ring (39) is used for wiring the injection pump motor (24), the liquid inlet changeover valve (13) and the liquid outlet changeover valve (14).

8. A high-speed single-rotation countercurrent chromatograph according to claim 5, characterized in that, A support bearing seat (40) is fixed on the device bottom plate (30). The bearing mounting ring (29) is rotatably mounted on the support bearing seat (40) through a support bearing (41).

9. A high-speed single-rotation countercurrent chromatograph according to claim 1, characterized in that, The end face of the spiral tube mounting bottom plate (2) provided with the spiral tube (3) is connected with a spiral tube mounting cover plate (42) by screws. The spiral tube (3) is limited between the spiral tube mounting bottom plate (2) and the spiral tube mounting cover plate (42). Central holes are formed in both the spiral tube mounting cover plate (42) and the spiral tube mounting bottom plate (2).

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