Method for artificial breeding of aplysia in indoor cement pond

Abstract

The present application relates to the field of aquaculture breeding technology, and discloses an indoor cement pool artificial breeding method for east wind snail, which comprises the following steps: storing east wind snail egg sacs after disinfecting water body by ultraviolet rays, removing infected egg sacs, and performing specific water flow replacement 24 hours before hatching; feeding the larvae with a nutrient agent containing clostridium aceticum protein and agaricus blazei polysaccharide every day after hatching, feeding the larvae with monosomic algae such as chrysomonad, tetraselmis, chlorella, etc. in the early stage, and feeding the larvae with shelled algae in the middle stage until the larvae are attached and metamorphosed; keeping the breeding pool continuously aerated and controlling parameters such as dissolved oxygen, salinity, temperature and pH value of the water body during the breeding period, and finally collecting the snail seedlings to end the breeding. The indoor cement pool artificial breeding method for east wind snail provided by the present application is suitable for one or more of fasciolaria fasciolaris and mud east wind snail, and can effectively improve the success rate and seedling quality of indoor cement pool breeding of east wind snail.

Description

Technical Field

[0001] This invention relates to the field of aquatic breeding technology, and in particular to a method for artificial seedling raising of *Sinocyclocheilus edulis* in indoor cement ponds. Background Technology

[0002] As a marine shellfish with high economic value, the whelk occupies an important position in the aquaculture market. Its delicious meat and rich nutrition make it popular with consumers, and market demand continues to grow. However, in the process of whelk farming, the supply of seedlings has always been a key bottleneck restricting the large-scale development of the industry. Traditional whelk seedling cultivation methods face many problems: seedlings collected from natural sea areas are severely affected by seasonal and environmental changes and fluctuations in resource availability, resulting in unstable seedling quantities that are difficult to meet the needs of large-scale farming; at the same time, the quality of wild-collected seedlings varies greatly, and they may carry various pathogens, leading to frequent disease outbreaks and low survival rates during farming, increasing farming risks and costs.

[0003] In addition, some existing indoor seedling cultivation technologies also have shortcomings. For example, the seedling facilities and methods are not perfect, and there is a lack of systematic and scientific solutions in terms of water quality control, feed feeding, and optimization of the larval rearing environment. This results in low seedling efficiency, long seedling cycle, and limited unit yield, making it difficult to achieve efficient and stable industrialized seedling production.

[0004] Based on the above situation, there is an urgent need for an innovative indoor cement pond artificial seedling cultivation method for *Bambusa multiplex* that can overcome the drawbacks of traditional seedling cultivation methods, achieve stable, efficient, and high-quality production of *Bambusa multiplex* seedlings, promote the sustainable and healthy development of the *Bambusa multiplex* aquaculture industry, meet the growing market demand, and provide farmers with a reliable seedling guarantee. Summary of the Invention

[0005] To address the aforementioned technical problems, the primary objective of this invention is to provide a method for artificial seedling cultivation of *Cyprinus edulis* in indoor cement ponds.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solution:

[0007] This invention provides a method for artificially raising seedlings of the Oriental Wind Snail in an indoor cement pond, comprising the following steps: S1. The water is disinfected with ultraviolet light, and then the Oriental Wind Snail egg sacs are placed in the seedling pond. Infected egg sacs are removed, and the water is changed 24 hours before hatching.

[0008] S2. When the larvae hatch from the egg sac, nutrients are added daily. Initially, they are fed with unicellular algae, and in the middle stage, they are fed with shrimp pond algae until they attach and metamorphose.

[0009] S3. Collect snail seedlings and end the seedling cultivation.

[0010] Preferably, in step S1, the snail is one or more of the following: the spotted snail and the mud snail.

[0011] Preferably, in step S1, the infected oocyst is non-transparent and exhibits colors such as white, yellow, or red.

[0012] Preferably, in step S1, the water exchange refers to replacing 1 / 3 to 4 / 5 of the original water volume per day, and controlling the water flow rate at 1-3 m / s. 3 / h.

[0013] Preferably, in step S2, the unicellular algae refer to one or more of golden algae, flat algae, and chlorella.

[0014] Preferably, in step S2, the nutrient is Clostridium ethanolis protein and Agrocybe aegerita polysaccharide.

[0015] Preferably, the daily addition amount of Clostridium ethanol protein is 0.1-1.0 g / m³. 3 The daily dosage of tea tree mushroom polysaccharide is 0.1-1.0 g / m³. 3 .

[0016] In this invention, Clostridium ethanolae protein has multiple functions:

[0017] 1. Provides high-quality nutrition: High protein content: The crude protein content of Clostridium ethanol is as high as 80% or more, which provides an ample protein source for the growth and development of the East Wind Snail seedlings, helps the seedlings grow rapidly, increase weight and body length, and improve the survival rate and growth rate of seedlings.

[0018] 2. Enhance immune function: Improving immunity can help seedlings resist the invasion of pathogens, reduce the incidence of diseases, and enhance their resistance to adverse environments;

[0019] 3. Improve gut health: Increase the number of beneficial bacteria in the gut of aquatic seedlings, regulate the balance of gut microbiota, inhibit the growth and reproduction of harmful bacteria, and reduce the occurrence of intestinal diseases.

[0020] In this invention, the polysaccharide from *Agrocybe aegerita* has multiple functions:

[0021] 1. Enhanced Immunity: The polysaccharides in *Agrocybe aegerita* can stimulate the development of the immune system in *Bellamya aegerita* larvae, increasing the activity and number of their immune cells and enhancing their resistance to disease. During seedling cultivation, *Bellamya aegerita* larvae are susceptible to various pathogens; enhancing immunity helps reduce the incidence of disease and improve seedling survival rate.

[0022] 2. Promotes growth and development: Provides nutritional support for the growth of *Bellamya aegypti* larvae. *Agrocybe aegypti* polysaccharides contain various nutrients, such as carbohydrates and amino acids, which can be absorbed and utilized by *Bellamya aegypti* larvae as the material basis for their growth and development, promoting weight gain and length increase, and regulating their physiological metabolism.

[0023] 3. Improve gut health: Maintain gut microbiota balance. Tea tree mushroom polysaccharides can promote the growth and reproduction of beneficial bacteria in the intestines of *Bellamya aegyptiaca* larvae, thereby maintaining gut microbiota balance. A healthy gut microbiota helps improve the digestive and absorptive capacity of *Bellamya aegyptiaca* larvae, promoting the full utilization of nutrients.

[0024] 4. Enhance stress resistance: During the seedling cultivation of Oriental Wind Snails, the larvae may be affected by stress factors such as environmental changes and water quality fluctuations. Tea tree mushroom polysaccharides can help Oriental Wind Snail larvae better cope with these stress factors and enhance their stress resistance.

[0025] Preferably, during the seedling raising process, the seedling pond is continuously aerated, the dissolved oxygen in the water is greater than 4 mg / L, the salinity is 25-33‰, the water temperature is 25-30℃, and the pH is 8.0-8.6.

[0026] Furthermore, the present invention also proposes the application of the above-mentioned seedling raising method in the artificial seedling raising of *Bellamya aegyptiaca* in indoor cement ponds.

[0027] Compared with related technologies, the artificial seedling cultivation method for *Sinocyclocheilus edulis* in indoor cement ponds provided by this invention has the following beneficial effects:

[0028] 1. Improved seedling survival rate: Ultraviolet disinfection of the water and removal of infected oocysts effectively reduce pathogen damage to oocysts and larvae, lowering the risk of disease and improving seedling survival rate. Simultaneously, precise control of water environmental parameters in the nursery pond, such as dissolved oxygen greater than 4 mg / L, salinity 25-33‰, temperature 25-30℃, and pH 8.0-8.6, provides stable and suitable conditions for the growth and development of *Bellamya aegyptiacus*, reducing mortality caused by environmental fluctuations and significantly improving survival rate compared to traditional seedling cultivation methods.

[0029] 2. Optimize seedling nutrition supply: In the early stage of larval rearing, feeding with a variety of single-celled algae ensures a rich source of nutrition. In the middle stage, feeding with shrimp pond algae adapts to the changing needs of their growth. In addition, adding a specific amount of Clostridium ethanol and Agrocybe aegerita polysaccharide as nutrients every day helps to enhance the larvae's constitution, promote their healthy growth, and make the seedlings stronger and more vigorous, with better adaptability and resistance in subsequent breeding processes.

[0030] 3. Scientific water change management: A continuous water change operation is performed for the first 24 hours before hatching, with a daily water change volume of 1 / 3 to 4 / 5 of the original water volume, and the water flow rate controlled at 1-3 m / s. 3 A water exchange rate of / h ensures water quality, providing a good aquatic environment for egg sac hatching and larval growth, while avoiding adverse effects on larvae caused by excessive or insufficient water exchange, thus improving seedling production results.

[0031] 4. Strong controllability and wide applicability: The entire seedling cultivation process is carried out in indoor cement tanks, which facilitates manual control and management and reduces interference from natural environmental factors. This method is applicable to various species of whelks and has broad application prospects. It is conducive to promoting the standardization and large-scale development of artificial seedling cultivation technology for whelks, providing a stable and reliable seedling supply guarantee for the whelk farming industry, and improving the economic benefits of farming. Attached Figure Description

[0032] Figure 1 This is a state diagram of the Dongfeng snail egg sac hatching pond according to Embodiment 1 of the present invention;

[0033] Figure 2 This is a diagram showing the state of the egg sac of *Sinocyclocheilus dongfengensis* in Embodiment 1 of the present invention.

[0034] Figure 3 This is a diagram showing the state of planktonic larvae of the *Bellamya aegyptiacus* in Embodiment 1 of the present invention.

[0035] Figure 4 This is a diagram showing the state of the *Euphorbia tirucalli* seedlings harvested in Example 1 of the present invention. Detailed Implementation

[0036] The present invention will be further illustrated below with reference to specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods, and equipment used in the present invention are conventional reagents, methods, and equipment in this technical field.

[0037] Example 1: A method for artificial seedling cultivation of *Bellamya aegypti* in indoor cement ponds is as follows:

[0038] Indoor cement seedling ponds should be 3 meters long, 4 meters wide, and 1.2 meters deep. The pond walls and bottom should be smooth and flat, without cracks or leaks to prevent the larvae of the snail from hiding or being injured. The hatching pond should be continuously aerated, maintaining dissolved oxygen levels greater than 4 mg / L, salinity at 25-33‰, temperature at 25-30℃, and pH at 8.0-8.6. A micro-aeration device should be installed in the hatching pond to gently agitate the water and ensure sufficient oxygen supply to the egg sacs. During incubation, the egg sacs should be observed daily, and infected egg sacs that are white, yellow, or have mold spots should be removed promptly to prevent the spread of pathogens. Twenty-four hours before hatching, the water should be changed while maintaining a flow rate of 1-3 m / s. 3The water volume should be changed daily at a rate of 1 / 3 to 4 / 5 of the original volume to maintain water quality and promote successful larval hatching. Once the larvae hatch from their egg sacs, they should be fed initial feed promptly. The initial feed should be *Golden Algae*, a single-celled algae, fed 2-3 times daily. The feeding amount should be adjusted according to larval density and water quality, generally maintaining a concentration of 20,000-50,000 single-celled algae per ml. Simultaneously, *Clostridium ethanolica* protein (source: Beijing Shougang Langze Technology Co., Ltd.) and *Agrocybe aegerita* polysaccharide (source: Shaanxi Tianxingjian Biochemical Technology Co., Ltd.) should be added daily as nutritional supplements. The daily addition of *Clostridium ethanolica* protein is 0.6 g / ml. 3 The daily dosage of tea tree mushroom polysaccharide is 0.5g / m³. 3 Dissolve it and then evenly sprinkle it into the seedling pond to enhance the larvae's physical condition and immunity.

[0039] After 13 days of cultivation, the planktonic larvae metamorphose and land to become juvenile snails that crawl. The water level in the nursery pond is lowered to 0.5 meters using a siphon pipe and maintained at this level. The valve is opened to allow water to flow. During this period, palatable newly hatched brine shrimp larvae (about 460 micrometers) are fed daily for 5 consecutive days. Feeding is stopped on the 6th day. On the 7th day, the pond water is drained, and a 100-mesh mesh bag is placed over the outlet to collect the snail seedlings. 3.36 catties of snail seedlings are harvested.

[0040] Example 2: A method for artificial seedling cultivation of *Bellamya aegypti* in indoor cement ponds is as follows:

[0041] The difference between this embodiment and Embodiment 1 is that no ethanol Clostridium protein and tea tree mushroom polysaccharide are added to the seedling pond during the seedling raising process, and 1.44 catties of snail seedlings are harvested.

[0042] Example 3: A method for artificial seedling cultivation of *Bellamya spp.* in indoor cement ponds is as follows:

[0043] The difference between this embodiment and Embodiment 1 is that no ethanol Clostridium protein is added to the pond during the seedling raising process, and 3.15 catties of snail seedlings are harvested.

[0044] Example 4: A method for artificial seedling cultivation of *Bellamya aegypti* in indoor cement ponds is as follows:

[0045] The difference between this embodiment and Embodiment 1 is that no tea tree mushroom polysaccharide was added to the pond during the seedling cultivation process, and 2.82 jin of snail seedlings were harvested.

[0046] Example 5: A method for artificial seedling cultivation of *Bellamya aegypti* in indoor cement ponds is as follows:

[0047] The difference between this embodiment and embodiment 1 is that: during the seedling raising process, small algae were used as the initial feed in the pond, and 1.18 jin of snail seedlings were harvested.

[0048] Example 6: A method for artificial seedling cultivation of *Bellamya aegypti* in indoor cement ponds is as follows:

[0049] The difference between this embodiment and Embodiment 1 is that: Chlorella is used instead of shrimp pond algae in the seedling raising process, and 0.83 catties of snail seedlings are harvested.

[0050] This application uses shrimp pond algae, Clostridium ethanol, and Agrocybe aegerita polysaccharide for seedling cultivation, which have a certain synergistic effect. The results show that compared with adding them alone or in a non-optimized combination, the synergistic effect significantly improves the overall effect of the seedling cultivation of Agrocybe aegerita, greatly increases the yield of seedlings, and significantly improves the seedling cultivation benefits.

[0051] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A method for artificially raising seedlings of *Bellamya aegypti* in an indoor cement pond, characterized in that, Includes the following steps: S1. The water is disinfected with ultraviolet light. Then, the egg sacs of the East Wind Snail are stored in the nursery pond. Infected egg sacs are removed. The water is changed 24 hours before hatching. S2. When the larvae hatch from the egg sac, nutrients are added daily. Initially, golden algae are fed, and in the middle stage, shrimp pond algae are fed until they attach and metamorphose. The nutrients are Clostridium ethanol and Agrocybe aegerita polysaccharide. S3. Collect snail seedlings and end the seedling cultivation.

2. The method for artificial seedling cultivation of *Sinocyclocheilus edulis* in an indoor cement pond as described in claim 1, characterized in that, In step S1, the snail is a spotted snail or a mud snail.

3. The method for artificial seedling cultivation of *Sinocyclocheilus edulis* in indoor cement ponds as described in claim 1, characterized in that... In step S1, the infected oocysts are non-transparent and appear whitish, yellowish, or reddish.

4. The method for artificial seedling cultivation of *Sinocyclocheilus edulis* in indoor cement ponds as described in claim 1, characterized in that, In step S1, the water exchange refers to replacing 1 / 3 to 4 / 5 of the original water volume daily, with the water flow rate controlled at 1-3 m / s. 3 / h.

5. The method for artificial seedling cultivation of *Sinocyclocheilus edulis* in indoor cement ponds as described in claim 1, characterized in that... The daily addition amount of Clostridium ethanol protein is 0.1-1.0 g / m³. 3 The daily dosage of polysaccharides from *Agrocybe aegerita* mushrooms is 0.1-1.0 g / m³. 3 .

6. The method for artificial seedling cultivation of *Sinocyclocheilus edulis* in an indoor cement pond as described in claim 1, characterized in that, During the seedling stage, the seedling pond should be continuously aerated, with dissolved oxygen greater than 4 mg / L, salinity of 25-33‰, water temperature of 25-30℃, and pH of 8.0-8.6.

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