Ozonized oil and fructus forsythiae composite micro-capsule, preparation method thereof, mouth wash for antibacterial repair and preparation method of mouth wash
By encapsulating ozone oil and Forsythia extract in microcapsules and combining them in mouthwash, the problems of limited antibacterial effects and rich Chinese medicine flavors are solved, and a wider spectrum of antibacterial effects and better taste are achieved.
Patent Information
- Application Number
- CN202510223816.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-27
- Publication Date
- 2025-06-17
AI Technical Summary
The existing mouthwash has limitations in antibacterial effects, and the traditional Chinese medicine compound mouthwash has problems with strong Chinese medicine taste and allergic risks.
The microcapsules are encapsulated in the microcapsules by preparative method to form an ozone oil Forsythia composite microcapsules and added to the mouthwash.
It has achieved effective inhibition of a variety of bacteria, improved the antibacterial spectrum of mouthwash, improved the taste and odor, reduced the stimulation of traditional Chinese medicine ingredients to the mouth, and reduced the risk of allergies.
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Figure CN120154552A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of daily chemical products, relates to mouthwash, and particularly relates to an ozonated oil forsythia suspense composite microcapsule and a preparation method thereof, a mouthwash for antibacterial repair and a preparation method thereof. Background Art
[0002] Ozonated oil is derived from ozone (O3), which can oxidize and destroy the cell structure of microorganisms such as bacteria, viruses, and fungi, playing a role in sterilization and disinfection; forsythia suspense is a common Chinese herbal medicine, which contains components such as forsythoside and phillyrin, and has an inhibitory effect on common oral pathogenic bacteria such as streptococcus mutans, etc., can reduce bacterial contamination, and prevent oral inflammation.
[0003] Mouthwash is a cleaning product used for the oral cavity, which can reach every corner of the oral cavity, clean food residues and dirt in the oral cavity, reduce the possibility of bacterial growth; can effectively inhibit the bacteria that produce odors in the oral cavity, decompose the odors in the oral cavity, and make the breath fresh; some mouthwashes containing antibacterial components can prevent the occurrence of oral diseases. At present, there are many types of mouthwashes on the market, but their functional effects are single, and the antibacterial effect has limitations. There are also some traditional Chinese medicine compound mouthwashes with good effects, but they have problems such as a strong smell of traditional Chinese medicine and an increased possibility of allergy due to the presence of multiple traditional Chinese medicine components. Summary of the Invention
[0004] Aiming at the deficiencies of the existing technology, the purpose of the present invention is to provide an ozonated oil forsythia suspense composite microcapsule and a preparation method thereof, a mouthwash for antibacterial repair and a preparation method thereof. This mouthwash has an inhibitory effect on a variety of bacteria, can more comprehensively deal with problems caused by oral bacteria, maintain the health of oral mucosa, and has a good taste and smell.
[0005] In order to achieve the above purpose, the present invention adopts the following technical solutions to achieve:
[0006] An ozonated oil forsythia suspense composite microcapsule, the components include: 5-15 wt% of ozonated oil, 5-15 wt% of forsythia suspense extract, 10-25 wt% of gelatin, 5-15 wt% of arabic gum, and the balance is deionized water.
[0007] A preparation method of the ozonated oil forsythia suspense composite microcapsule as described above, comprising the following steps:
[0008] Step 1: Take gelatin and arabic gum according to the mass ratio, add them to deionized water respectively, heat in a water bath at 50-60 °C and stir until dissolved to obtain solution A and solution B with a mass fraction of 2.5%;
[0009] Step 2: Take ozonated oil and forsythia suspense extract according to the mass ratio and mix them, stir to form a uniform core material mixed solution C;
[0010] Step 3: Add the core material mixture C into solution A, stir for 30 - 60 min to form the primary emulsion D of water-in-oil type. Add solution B into the emulsion D, adjust the pH to 4.0 - 5.0 with glacial acetic acid, and continuously stir for 30 - 60 min to cause the complex coacervation reaction of gelatin and gum arabic to obtain an emulsion.
[0011] Step 4: Add a glutaraldehyde solution with a mass ratio of 0.05% - 0.2% into the emulsion prepared in Step 3, stir for 2 - 3 h, then adjust the pH to neutral with sodium hydroxide. Centrifuge and precipitate the obtained microcapsule solution and wash repeatedly until no glutaraldehyde residue can be detected, and then vacuum dry to constant weight to obtain the ozonated oil forsythia complex microcapsules.
[0012] Preferably, the stirring time in Step 1 and Step 2 is 15 - 30 min.
[0013] The stirring rate in Step 1 to Step 4 is 300 - 500 r / min.
[0014] Preferably, the mass percentage concentration of the glutaraldehyde solution in Step 4 is 25%.
[0015] Preferably, the vacuum drying temperature in Step 4 is 40 - 50 °C.
[0016] The present invention also protects a mouthwash containing the ozonated oil forsythia complex microcapsules for antibacterial repair, and the components include 1 - 5 wt% of the ozonated oil forsythia complex microcapsules as described above, 0.5 - 2 wt% of sodium carboxymethylcellulose, 0.1 - 1 wt% of a sweetening agent, 0.05 - 0.5 wt% of menthol, 0.1 - 0.3 wt% of sodium benzoate, and the balance is deionized water.
[0017] Preferably, the ozonated oil is a vegetable oil-based ozonated oil, specifically including any one of olive oil-based ozonated oil, sunflower seed oil-based ozonated oil, and sweet almond oil-based ozonated oil.
[0018] Preferably, the ozone content in the ozonated oil is 100 - 500 mg / L.
[0019] Preferably, the sweetening agent includes xylitol or sorbitol.
[0020] The present invention also protects a preparation method of a mouthwash containing the ozonated oil forsythia complex microcapsules for antibacterial repair as described above, including the following steps:
[0021] Add deionized water to a container, heat it to 60 - 70 °C, and slowly add sodium carboxymethylcellulose while stirring to form a uniform viscous solution. Cool it to 30 - 40 °C, add ozone - oxidized oil forsythia complex microcapsules according to the mass ratio, and stir to disperse them evenly in the solution. Then add a sweetening agent, menthol, and sodium benzoate in sequence, stir well. After adding the remaining deionized water and stirring evenly, homogenize it with a homogenizer to obtain a mouthwash containing ozone - oxidized oil forsythia complex microcapsules for antibacterial repair.
[0022] Compared with the prior art, the present invention has the following technical effects:
[0023] In the present invention, the ozone - oxidized oil forsythia is compounded into the form of microcapsules. The microcapsule form improves the stability of the ozone - oxidized oil, masks the smell of the ozone - oxidized oil, and improves the acceptance of users. At the same time, the taste of the forsythia extract, a traditional Chinese medicine ingredient, is sealed in the microcapsules, reducing its impact on the smell and taste of the mouthwash, improving the taste and odor of the traditional Chinese medicine mouthwash. Components such as gelatin, gum arabic, sweetening agent, menthol, and sodium benzoate in the mouthwash can also form a protective film on the surface of the oral mucosa, relieve oral dryness, and enhance the comfort and compliance of use.
[0024] The ozone - oxidized oil has strong oxidizing properties, which can destroy the cell membranes and genetic materials of microorganisms such as bacteria and viruses, achieving efficient sterilization. At the same time, it has a certain anti - inflammatory effect, which can relieve oral mucosal inflammation and promote mucosal repair. The forsythia extract is rich in active ingredients such as forsythoside and phillyrin, which have the effect of clearing heat and detoxifying, and can relieve the swelling and pain of the oral mucosa.
[0025] The combination of the ozone - oxidized oil with strong oxidizing properties and forsythia rich in various antibacterial components realizes the complementary advantages of natural ingredients, has an inhibitory effect on a variety of bacteria, makes the antibacterial spectrum wider, can more comprehensively deal with problems caused by oral bacteria, can maintain the health of the oral mucosa while cleaning the oral cavity, and reduces the occurrence of allergic reactions. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] Figure 1 It is a physical picture of the ozone - oxidized oil forsythia complex microcapsules before drying prepared in Example 1;
[0027] Figure 2 It is a physical picture of the ozone - oxidized oil forsythia complex microcapsules prepared in Example 1;
[0028] Figure 3 It is a physical picture of the mouthwash containing ozone - oxidized oil forsythia complex microcapsules for antibacterial repair prepared in Example 1. DETAILED DESCRIPTION OF THE INVENTION
[0029] The following further elaborates on the specific content of the present invention in conjunction with examples.
[0030] The reagents, methods, and equipment used in the present invention are conventional reagents, methods, and equipment in the technical field. For the test methods without specific experimental conditions noted in the following examples, they are usually carried out according to conventional experimental conditions. Unless otherwise specified, the reagents and raw materials used in the present invention can be obtained commercially.
[0031] The ozonated oil used in the present invention is commercially available ozonated oil, with an ozone content of 100 - 500 mg / L;
[0032] The mass percentage concentration of the glutaraldehyde solution used in each of the following examples is 25%;
[0033] The extraction method of forsythia extract in the present invention includes the following steps:
[0034] Step 1: Take ethanol with a mass concentration of 50% and forsythia powder in a mass ratio of 5:1, mix and shake until evenly dispersed to obtain a dispersion liquid. Ultrasonically extract the dispersion liquid for 30 min and then perform vacuum filtration under reduced pressure. Repeat the above operations 3 times and combine the filtrates;
[0035] Step 2: Concentrate the filtrate from Step 1 using a rotary evaporator to remove ethanol, then mix water-saturated n-butanol and the filtrate in a volume ratio of 1:1 for extraction. Repeat 3 times, combine the organic phases and perform distillation and concentration to dryness using a rotary evaporator to obtain forsythia extract.
[0036] Example 1:
[0037] This example provides a mouthwash containing ozonated oil forsythia composite microcapsules for antibacterial repair, and the components include: 2 wt% of ozonated oil forsythia composite microcapsules, 0.5 wt% of sodium carboxymethylcellulose, 0.1 wt% of xylitol, 0.05 wt% of menthol, 0.1 wt% of sodium benzoate, and the balance is deionized water;
[0038] The components of the ozonated oil forsythia composite microcapsules include: 5 wt% of olive oil-based ozonated oil, 10 wt% of forsythia extract, 10 wt% of gelatin, 5 wt% of arabic gum, and the balance is deionized water;
[0039] The specific preparation steps are as follows:
[0040] Step 1: Preparation of ozonated oil forsythia composite microcapsules
[0041] Take gelatin and gum arabic, add them separately into deionized water, heat in a water bath at 50 °C and stir at 300 r / min for 15 min until dissolved to obtain solution A and solution B with a mass fraction of 2.5%; take ozonated oil and forsythia extract, mix and stir for 15 min to form a uniform core material mixture C; add the core material mixture C into solution A under stirring, maintain the stirring speed at 300 r / min and continue stirring for 40 min to form a water-in-oil primary emulsion D. Add solution B into emulsion D, maintain the stirring speed and continue stirring for 40 min, adjust the pH to 4.0 with glacial acetic acid, and gelatin and gum arabic will undergo complex coacervation reaction to produce microcapsules;
[0042] Step 2: Curing of the ozonated oil forsythia composite microcapsules
[0043] Add a glutaraldehyde solution with a mass ratio of 0.2%, stir for 2 h, adjust the pH to neutral with sodium hydroxide to terminate the reaction; centrifuge and precipitate the obtained microcapsule solution at 4000 r / min and wash repeatedly until no glutaraldehyde residue can be detected; then dry in vacuum at 50 °C to constant weight to obtain the ozonated oil forsythia composite microcapsules;
[0044] Step 3: Preparation of the mouthwash
[0045] Add deionized water into a container, heat to 60 °C, and slowly add sodium carboxymethylcellulose under stirring at 300 r / min to form a uniform viscous solution; cool the solution to 30 °C, add the ozonated oil forsythia composite microcapsules, and stir for 30 min to evenly disperse the microcapsules in the solution; sequentially add xylitol, menthol, and sodium benzoate, stir for 15 min until uniform to fully dissolve and mix the components; add the remaining deionized water, stir for 15 min until uniform, and then homogenize with a homogenizer for 5 min to obtain the mouthwash.
[0046] Example 2:
[0047] This example provides a mouthwash containing ozonated oil forsythia composite microcapsules for antibacterial repair, and the components include: 3 wt% of ozonated oil forsythia composite microcapsules, 0.6 wt% of sodium carboxymethylcellulose, 0.3 wt% of xylitol, 0.07 wt% of menthol, 0.2 wt% of sodium benzoate, and the balance is deionized water;
[0048] The components of the ozonated oil forsythia composite microcapsules include: 5 wt% of sunflower oil-based ozonated oil, 13 wt% of forsythia extract, 12 wt% of gelatin, 6 wt% of gum arabic, and the balance is deionized water.
[0049] The specific preparation steps are as follows:
[0050] Step 1: Preparation of the ozonated oil forsythia composite microcapsules
[0051] Take gelatin and gum arabic, add them into deionized water respectively, heat in a water bath at 55 °C and stir at 300 r / min for 30 min until dissolved to obtain solution A and solution B with a mass fraction of 2.5%; take ozonated oil and forsythia extract, mix and stir for 30 min to form a uniform core material mixture C; add the core material mixture C into solution A under stirring, maintain the stirring speed at 300 r / min and continue stirring for 30 min to form a water-in-oil primary emulsion D. Add solution B into emulsion D, maintain the stirring speed and continue stirring for 60 min, adjust the pH to 4.5 with glacial acetic acid, and gelatin and gum arabic will undergo complex coacervation reaction to produce microcapsules;
[0052] Step 2: Curing of ozonated oil forsythia composite microcapsules
[0053] Add a glutaraldehyde solution with a mass ratio of 0.05%, stir for 2 h, adjust the pH to neutral with sodium hydroxide to terminate the reaction; centrifuge and precipitate the obtained microcapsule solution at 4000 r / min and wash repeatedly until no glutaraldehyde residue can be detected; then dry in vacuum at 50 °C to constant weight to obtain ozonated oil forsythia composite microcapsules;
[0054] Step 3: Preparation of mouthwash
[0055] Add deionized water into a container, heat to 60 °C, and slowly add sodium carboxymethylcellulose under stirring at 300 r / min to form a uniform viscous solution; cool the solution to 30 °C, add ozonated oil forsythia composite microcapsules, and stir for 30 min to evenly disperse the microcapsules in the solution; sequentially add xylitol, menthol, and sodium benzoate, stir for 15 min until uniform to fully dissolve and mix each component; add the remaining deionized water, stir for 15 min until uniform, and then homogenize with a homogenizer for 5 min to obtain mouthwash.
[0056] Example 3:
[0057] This example provides a mouthwash containing ozonated oil forsythia composite microcapsules for antibacterial repair, and the components include: 5 wt% of ozonated oil forsythia composite microcapsules, 0.5 wt% of sodium carboxymethylcellulose, 0.9 wt% of xylitol, 0.4 wt% of menthol, 0.2 wt% of sodium benzoate, and the balance is deionized water;
[0058] The components of the ozonated oil forsythia composite microcapsules include: 8 wt% of sweet almond oil-based ozonated oil, 8 wt% of forsythia extract, 10 wt% of gelatin, 5 wt% of gum arabic, and the balance is deionized water;
[0059] The specific preparation steps are as follows:
[0060] Step 1: Preparation of ozonated oil forsythia composite microcapsules
[0061] Take gelatin and gum arabic, add them separately into deionized water, heat in a water bath at 55 °C and stir at 400 r / min for 20 min until dissolved to obtain solution A and solution B with a mass fraction of 2.5%; take ozonated oil and forsythia extract, mix and stir for 20 min to form a uniform core material mixture C; add the core material mixture C into solution A under stirring, maintain the stirring speed of 400 r / min and continue stirring for 30 min to form a water-in-oil primary emulsion D, add solution B into emulsion D, maintain the stirring speed and continue stirring for 50 min, adjust the pH to 5.0 with glacial acetic acid, and gelatin and gum arabic will undergo complex coacervation reaction to produce microcapsules;
[0062] Step 2: Curing of the ozonated oil forsythia composite microcapsules
[0063] Add a glutaraldehyde solution with a mass ratio of 0.1%, stir for 2 h, adjust the pH to neutral with sodium hydroxide to terminate the reaction; centrifuge and precipitate the obtained microcapsule solution at 4000 r / min and wash repeatedly until no glutaraldehyde residue can be detected; then dry in vacuum at 50 °C to constant weight to obtain the ozonated oil forsythia composite microcapsules;
[0064] Step 3: Preparation of the mouthwash
[0065] Add deionized water into a container, heat to 60 °C, and slowly add sodium carboxymethylcellulose under stirring at 300 r / min to form a uniform viscous solution; cool the solution to 30 °C, add the ozonated oil forsythia composite microcapsules, and stir for 30 min to evenly disperse the microcapsules in the solution; sequentially add xylitol, menthol, and sodium benzoate, and stir for 15 min until uniform to fully dissolve and mix the components; add the remaining deionized water, stir for 15 min until uniform, and then homogenize with a homogenizer for 5 min to obtain the mouthwash.
[0066] Example 4:
[0067] This example provides a mouthwash containing ozonated oil forsythia composite microcapsules for antibacterial repair, and the components include: 2 wt% of ozonated oil forsythia composite microcapsules, 0.7 wt% of sodium carboxymethylcellulose, 0.2 wt% of sorbitol, 0.2 wt% of menthol, 0.1 wt% of sodium benzoate, and the balance is deionized water;
[0068] The components of the ozonated oil forsythia composite microcapsules include: 10 wt% of olive oil-based ozonated oil, 12 wt% of forsythia extract, 14 wt% of gelatin, 10 wt% of gum arabic, and the balance is deionized water;
[0069] The specific preparation steps are as follows:
[0070] Step 1: Preparation of the ozonated oil forsythia composite microcapsules
[0071] Take gelatin and gum arabic, add them into deionized water respectively, heat in a water bath at 60°C and stir at 400 r / min for 20 min until dissolved to obtain solution A and solution B with a mass fraction of 2.5%; take ozonated oil and forsythia extract, mix and stir for 30 min to form a uniform core material mixture C; add the core material mixture C into solution A under stirring, maintain the stirring speed at 400 r / min and continue stirring for 60 min to form a water-in-oil primary emulsion D. Add solution B into emulsion D, maintain the stirring speed and continue stirring for 30 min, adjust the pH to 4.5 with glacial acetic acid, and gelatin and gum arabic will undergo complex coacervation reaction to produce microcapsules;
[0072] Step 2: Curing of ozonated oil forsythia composite microcapsules
[0073] Add a glutaraldehyde solution with a mass ratio of 0.08%, stir for 2 h, adjust the pH to neutral with sodium hydroxide to terminate the reaction; centrifuge and precipitate the obtained microcapsule solution at 4000 r / min and wash repeatedly until no glutaraldehyde residue can be detected; then dry in vacuum at 50°C to constant weight to obtain ozonated oil forsythia composite microcapsules;
[0074] Step 3: Preparation of mouthwash
[0075] Add deionized water into a container, heat to 60°C, and slowly add sodium carboxymethylcellulose while stirring at 300 r / min to form a uniform viscous solution; cool the solution to 30°C, add ozonated oil forsythia composite microcapsules, and stir for 30 min to evenly disperse the microcapsules in the solution; sequentially add sorbitol, menthol, and sodium benzoate, stir for 15 min until uniform to fully dissolve and mix the components; add the remaining deionized water, stir for 15 min until uniform, and then homogenize with a homogenizer for 5 min to obtain mouthwash.
[0076] Example 5:
[0077] This example provides a mouthwash containing ozonated oil forsythia composite microcapsules for antibacterial repair, and the components include: 3 wt% of ozonated oil forsythia composite microcapsules, 1 wt% of sodium carboxymethylcellulose, 0.5 wt% of sorbitol, 0.2 wt% of menthol, 0.2 wt% of sodium benzoate, and the balance is deionized water;
[0078] The components of the ozonated oil forsythia composite microcapsules include: 12 wt% of olive oil-based ozonated oil, 7 wt% of forsythia extract, 10 wt% of gelatin, 5 wt% of gum arabic, and the balance is deionized water;
[0079] The specific preparation steps are as follows:
[0080] Step 1: Preparation of ozonated oil forsythia composite microcapsules
[0081] Take gelatin and gum arabic, add them to deionized water respectively, heat in a water bath at 55 °C and stir at 350 r / min for 30 min until dissolved to obtain solution A and solution B with a mass fraction of 2.5%; take ozonated oil and forsythia extract, mix and stir for 30 min to form a uniform core material mixture C; add the core material mixture C to solution A under stirring, maintain the stirring speed at 350 r / min and continue stirring for 30 min to form a water-in-oil primary emulsion D, add solution B to the emulsion D, maintain the stirring speed and continue stirring for 60 min, adjust the pH to 5.0 with glacial acetic acid, and gelatin and gum arabic will undergo complex coacervation reaction to produce microcapsules;
[0082] Step 2: Curing of the ozonated oil forsythia composite microcapsules
[0083] Add a glutaraldehyde solution with a mass ratio of 0.15%, stir for 2.5 h, adjust the pH to neutral with sodium hydroxide to terminate the reaction; centrifuge and precipitate the obtained microcapsule solution at 4000 r / min and wash repeatedly until no glutaraldehyde residue can be detected; then vacuum dry at 40 °C to constant weight to obtain the ozonated oil forsythia composite microcapsules;
[0084] Step 3: Preparation of the mouthwash
[0085] Add deionized water to a container, heat to 65 °C, slowly add sodium carboxymethylcellulose under stirring at 300 r / min to form a uniform viscous solution; cool the solution to 35 °C, add the ozonated oil forsythia composite microcapsules, and stir for 15 min to evenly disperse the microcapsules in the solution; sequentially add sorbitol, menthol, and sodium benzoate, stir for 30 min until uniform to fully dissolve and mix the components; add the remaining deionized water, stir for 30 min until uniform, and then homogenize with a homogenizer for 20 min to obtain the mouthwash.
[0086] Example 6:
[0087] This example provides a mouthwash containing ozonated oil forsythia composite microcapsules for antibacterial repair, and the components include: 1 wt% of ozonated oil forsythia composite microcapsules, 2 wt% of sodium carboxymethylcellulose, 1 wt% of sorbitol, 0.5 wt% of menthol, 0.3 wt% of sodium benzoate, and the balance is deionized water;
[0088] The components of the ozonated oil forsythia composite microcapsules include: 15 wt% of olive oil-based ozonated oil, 15 wt% of forsythia extract, 25 wt% of gelatin, 15 wt% of gum arabic, and the balance is deionized water;
[0089] The specific preparation steps are as follows:
[0090] Step 1: Preparation of the ozonated oil forsythia composite microcapsules
[0091] Take gelatin and gum arabic, add them into deionized water respectively, heat in a water bath at 55 °C and stir at 500 r / min for 30 min until dissolved to obtain solution A and solution B with a mass fraction of 2.5%; take ozonated oil and forsythia extract and mix and stir for 30 min to form a uniform core material mixture C; add the core material mixture C into solution A under stirring, maintain the stirring speed of 500 r / min and continue stirring for 30 min to form a water-in-oil primary emulsion D, add solution B into the emulsion D, maintain the stirring speed and continue stirring for 60 min, adjust the pH to 5.0 with glacial acetic acid, and gelatin and gum arabic will undergo complex coacervation reaction to produce microcapsules;
[0092] Step 2: Curing of the ozonated oil forsythia composite microcapsules
[0093] Add a glutaraldehyde solution with a mass ratio of 0.2%, stir for 3 h, adjust the pH to neutral with sodium hydroxide to terminate the reaction; centrifuge and precipitate the obtained microcapsule solution at 4000 r / min and wash repeatedly until no glutaraldehyde residue can be detected; then dry in vacuum at 45 °C to constant weight to obtain the ozonated oil forsythia composite microcapsules;
[0094] Step 3: Preparation of the mouthwash
[0095] Add deionized water into a container, heat to 70 °C, and slowly add sodium carboxymethylcellulose while stirring at 300 r / min to form a uniform viscous solution; cool the solution to 40 °C, add the ozonated oil forsythia composite microcapsules, and stir for 20 min to make the microcapsules evenly disperse in the solution; sequentially add sorbitol, menthol, and sodium benzoate, and stir for 20 min until uniform to fully dissolve and mix the components; add the remaining deionized water, stir for 20 min until uniform, and then homogenize for 10 min with a homogenizer to obtain the mouthwash.
[0096] The paper disk diffusion method was used to detect the antibacterial rates of the mouthwashes containing ozonated oil forsythia microcapsules for antibacterial repair in Examples 1 to 5 against Staphylococcus aureus, Streptococcus mutans, and Porphyromonas gingivalis. Prepare the bacterial suspension, dip a sterile cotton swab into the bacterial suspension and evenly coat it on the surface of the agar plate. Stick the filter paper soaked with the mouthwash on the coated plate, invert the plate and place it in an incubator, and culture at 37 °C for 18 - 24 hours. After the culture is completed, measure the diameter of the antibacterial circle and calculate the antibacterial rate. The results are shown in Table 1.
[0097] As can be seen from Table 1, the mouthwashes containing ozonated oil forsythia microcapsules for antibacterial repair of the present invention have good inhibitory effects on Staphylococcus aureus, Streptococcus mutans, and Porphyromonas gingivalis.
[0098] Table 1 Antibacterial evaluation of the mouthwashes prepared in Examples 1 to 5
[0099]
[0100] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; without departing from the concept of the present invention, any deductions or substitutions made by those skilled in the art belong to the protection scope of the present invention.
Claims
1. An ozonated oil Forsythia composite microcapsule, characterized in that: The components include: 5-15wt% of ozonated oil, 5-15wt% of forsythia extract, 10-25wt% of gelatin, 5-15wt% of gum arabic, and the balance is deionized water.
2. A method for preparing ozonated oil Forsythia composite microcapsules as claimed in claim 1, characterized in that: The following steps are involved: Step 1: Take gelatin and gum arabic according to the mass ratio, add them into deionized water respectively, heat in a water bath at 50-60°C and stir until dissolved, to obtain solution A and solution B with a mass fraction of 2.5%; Step 2: Mix the ozonated oil and the Forsythia suspensa extract according to the mass ratio, and stir to form a uniform core material mixed solution C; Step 3, adding the core material mixture C to the solution A, stirring for 30 to 60 minutes to form an oil-in-water type primary emulsion D, adding the solution B to the emulsion D, adjusting the pH to 4.0 to 5.0 with glacial acetic acid, and continuously stirring for 30 to 60 minutes to allow the gelatin and gum arabic to undergo a complex coagulation reaction to obtain an emulsion; Step 4: Add 0.05% to 0.2% by weight of glutaraldehyde solution to the emulsion prepared in step 3, stir for 2 to 3 hours, then adjust the pH to neutral with sodium hydroxide, centrifuge the obtained microcapsule solution and repeatedly wash until no glutaraldehyde residue is detected, and then vacuum dry to constant weight to obtain ozonated oil Forsythia composite microcapsules.
3. The method for preparing the ozonated oil Forsythia composite microcapsules according to claim 2, characterized in that: The stirring time in step 1 and step 2 is 15 to 30 minutes; The stirring rate in step 1 to step 4 is 300-500 r / min.
4. The method for preparing the ozonated oil Forsythia composite microcapsules according to claim 2, characterized in that: The mass percentage concentration of the glutaraldehyde solution described in step 4 is 25%.
5. The method for preparing the ozonated oil Forsythia composite microcapsules according to claim 2, characterized in that: The vacuum drying temperature described in step 4 is 40-50°C.
6. A mouthwash containing ozonated oil Forsythia composite microcapsules for antibacterial repair, characterized in that: The components include 1-5wt% of the ozonated oil forsythia composite microcapsule as claimed in claim 1, 0.5-2wt% of sodium hydroxymethyl cellulose, 0.1-1wt% of sweetener, 0.05-0.5wt% of menthol, 0.1-0.3wt% of sodium benzoate, and the balance is deionized water.
7. The mouthwash containing ozonated oil Forsythia composite microcapsules for antibacterial repair according to claim 6, characterized in that: The ozonated oil is a vegetable oil-based ozonated oil, specifically including any one of olive oil-based ozonated oil, sunflower oil-based ozonated oil and sweet almond oil-based ozonated oil.
8. The mouthwash containing ozonated oil Forsythia composite microcapsules for antibacterial repair according to claim 6, characterized in that: The ozone content in the ozonated oil is 100-500 mg / L.
9. The mouthwash containing ozonated oil Forsythia composite microcapsules for antibacterial repair according to claim 6, characterized in that: The sweetener includes xylitol or sorbitol.
10. A method for preparing a mouthwash containing ozonated oil Forsythia composite microcapsules for antibacterial repair according to any one of claims 6 to 9, characterized in that: The following steps are involved: Deionized water is added to a container, heated to 60-70° C., sodium carboxymethyl cellulose is slowly added under stirring to form a uniform viscous solution, cooled to 30-40° C., ozonated oil Forsythia composite microcapsules are added according to the mass ratio and stirred to be uniformly dispersed in the solution; sweetener, menthol and sodium benzoate are added in sequence and stirred evenly, the remaining amount of deionized water is added and stirred evenly, and then homogenized with a homogenizer to obtain a mouthwash containing ozonated oil Forsythia composite microcapsules for antibacterial repair.