Cell culture device adaptable to culture dishes with various diameters

Abstract

The invention relates to the technical field of cell culture, and particularly discloses a cell culture device adaptable to culture dishes with various diameters, the cell culture device comprises a device shell, an oscillation assembly and a water bath box, the oscillation assembly comprises an oscillation mechanism and an oscillation rod, the oscillation rod is fixedly connected with the output end of the oscillation mechanism, and a clamping assembly is arranged in the device shell; the clamping assembly comprises a connecting rod fixedly connected to the oscillating rod. When a culture dish enters a station, a first clamping belt generates stretching deformation under the elastic effect of a first spring, the first clamping belt and a second clamping belt generate initial clamping force under the synergistic effect of a second spring, pre-positioning of the periphery of the culture dish is achieved, and then a fixing block drives a connecting belt to apply tension loading on the first clamping belt; a wedge-shaped pushing block is synchronously linked to push a clamping block to adjust the tension of a clamping belt II, stable clamping of the culture dishes is completed through the coupling effect of elastic deformation of a belt body and mechanical limiting, the clamping stability of the culture dishes of different specifications is ensured, and the situation that the culture environment is affected by shaking is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and particularly relates to a cell culture device adaptable to culture dishes of various diameters. Background Art

[0002] A cell culture device is a professional device for cell culture. Under the action of the oscillation function, the cell culture medium in the culture container is continuously mixed and flows, enabling the cells to fully contact nutrients and oxygen, while discharging metabolic wastes, simulating the physiological environment of cells in the organism, and promoting the growth and reproduction of cells.

[0003] The cell water bath culture device automatically adapts to and fixes culture dishes of different diameters through an elastic clamping assembly, ensuring that culture dishes of various specifications can be stably placed. When using a large-diameter culture dish for oscillation culture, two problems will occur: First, a larger oscillation amplitude will cause higher waves in the water bath, which may cause the water bath liquid to splash out of the device or enter the culture dish; Second, the mass inertia of the large-diameter culture dish itself will weaken the adaptability stability of the elastic clamping assembly, ultimately causing the culture dish to shake during the oscillation process, and this shake will interfere with the microenvironment of cell culture.

[0004] Therefore, it is necessary to provide a cell culture device adaptable to culture dishes of various diameters, aiming to solve the above problems. Summary of the Invention

[0005] The main purpose of the present invention is to provide a cell culture device adaptable to culture dishes of various diameters, which can effectively solve the problems in the background art.

[0006] To achieve the above purpose, the technical solution adopted by the present invention is as follows: A cell culture device adaptable to culture dishes of various diameters includes a device housing, an oscillation assembly, and a water bath tank. The oscillation assembly includes an oscillation mechanism and an oscillation rod. The oscillation rod is fixedly connected to the output end of the oscillation mechanism. A clamping assembly is arranged inside the device housing. The clamping assembly includes a connecting rod fixedly connected to the oscillation rod. Clamping seats are installed between the connecting rods through an installation mechanism. Clamping mechanism one and clamping mechanism two are arranged inside the clamping seats. Buffer mechanisms are symmetrically arranged between the device housing and the water bath tank; Clamping mechanism one includes a clamping belt one, a fixed seat, a spring one, a connecting belt, a fixed block, a clamping seat, and a movable plate. The clamping seats are symmetrically and fixedly connected to the outer sides of both ends of the clamping belt one. The spring one is fixedly connected between the movable plate and the fixed seat. The connecting belts are symmetrically and fixedly connected between the movable plate and the clamping seats. The fixed block is used for clamping and fixing the connecting belt; The second clamping mechanism includes a second clamping belt, a limit seat, a connecting block, a wedge-shaped push block, a clamping block, a second spring and a movable seat. The second clamping belt is slidably connected to the inner side of the movable seat through the clamping block. The second spring is fixedly connected between the movable seats and between the movable seat and the inner wall of the limit seat. The connecting block and the wedge-shaped push block are used for clamping and fixing the movable seat.

[0007] As a further improvement of the above solution, a splash-proof component is provided at the top of the device housing. The splash-proof component includes a support seat and an upper cover. A support block is fixedly connected to the top of the support seat. Connecting shafts are symmetrically and fixedly connected to the inside of the upper cover. The upper cover is rotatably connected to the top of the support block through the connecting shafts. The connecting rod penetrates through the support seat through a sealing mechanism.

[0008] As a further improvement of the above solution, limiting rods are evenly and fixedly connected between the fixed seat and the clamping seat. The movable plate is slidably connected to the outside of the limiting rods. A circular groove adapted to the connecting belt is provided on the outside of the fixed block.

[0009] As a further improvement of the above solution, the clamping blocks are symmetrically and fixedly connected to both ends of the second clamping belt. A chute is provided inside the movable seat. The second clamping mechanism is slidably connected to the inside of the chute. Card slots are symmetrically provided on the outside of the movable seat. The connecting block is slidably connected to the inside of the card slot. The wedge-shaped push block is fixedly connected to the connecting block. The wedge-shaped push block penetrates into the chute inside the movable seat. The connecting block is fixedly connected to the fixed block.

[0010] As a further improvement of the above solution, the installation mechanism includes a mounting seat fixedly connected to the bottom of the connecting rod. The mounting seat is slidably connected to the outside of the clamping seat. A driving shaft is rotatably connected to the inside of the connecting rod. A bidirectional lead screw is fixedly connected to the outside of the driving shaft. Movable blocks are symmetrically and slidably connected to the inside of the mounting seat. The two movable blocks are symmetrically threadedly connected to the outside of the bidirectional lead screw. The connecting block is fixedly connected to the movable block.

[0011] As a further improvement of the above solution, the buffer mechanism includes a sealing seat fixedly connected between the device housing and the water bath tank. A piston is slidably connected to the inside of the sealing seat. Support sleeves are symmetrically and fixedly connected to the side of the piston close to the inner wall of the device housing. A third spring is fixedly connected between the support sleeve and the inner wall of the device housing. Conical holes with larger inner diameters and smaller outer diameters are evenly provided at the bottom of the water bath tank.

[0012] As a further improvement of the above solution, the sealing mechanism includes a mounting plate and a sealing sleeve. The mounting plate is fixedly connected to the sealing sleeve. The sealing sleeve is slidably connected to the inside of the support seat. A sealing plate is slidably connected to the inside of the sealing sleeve. The sealing plate is fixedly connected to the top of the connecting rod.

[0013] Compared with the prior art, the present invention has the following beneficial effects: 1. When the culture dish enters the working station, the first clamping belt generates a tensile deformation under the elastic action of the first spring, and forms an initial clamping force in cooperation with the second clamping belt under the second spring, realizing the pre-positioning of the outer periphery of the culture dish. Subsequently, the connecting belt is driven by the fixed block to apply a tension load to the first clamping belt, and the synchronous linkage wedge-shaped push block pushes the block to adjust the tension of the second clamping belt. The stable clamping of the culture dish is completed through the coupling effect of the elastic deformation of the belt body and the mechanical limit, ensuring the clamping stability of culture dishes of different specifications and avoiding shaking from affecting the culture environment. 2. When there are too many culture dishes or the specifications exceed the threshold, the liquid level in the water bath tank rises, resulting in an increase in the bottom static pressure. This pressure is transmitted to the piston through the conical throttle hole, driving it to compress the third spring to generate displacement, thereby triggering the liquid level adjustment to make the liquid level drop. In addition, during oscillation, the liquid reflux can be effectively reduced through the conical hole. Moreover, the top of the device is composed of a support seat and a sealed upper cover to form a double protection structure, thereby effectively suppressing the splashing of liquid oscillation. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0015] Figure 1 is the overall structural schematic diagram of the present invention; Figure 2 is the internal structural schematic diagram of the device housing of the present invention; Figure 3 is the structural schematic diagram of the buffer mechanism of the present invention; Figure 4 is the structural schematic diagram of the water bath tank of the present invention; Figure 5 is the top structural schematic diagram of the device housing of the present invention; Figure 6 is the structural schematic diagram of the anti-splash component and the clamping component of the present invention; Figure 7 is the structural schematic diagram of the sealing mechanism of the present invention; Figure 8 is the structural schematic diagram of the clamping component of the present invention; Figure 9 is the present invention Figure 8 the structural schematic diagram at A in; Figure 10 is the structural schematic diagram of the clamping seat of the present invention; Figure 11 is the structural schematic diagram of the fixed block and the connecting block of the present invention; Figure 12 Schematic diagram of the internal structure of the clamping seat of the present invention; Figure 13 For the present invention Figure 12 Schematic diagram of the structure at position B in; Figure 14 Schematic cross-sectional structure diagram of the clamping seat of the present invention; Figure 15 For the present invention Figure 14 Schematic diagram of the structure at position C in; Figure 16 Schematic diagram of the structure of the second clamping mechanism of the present invention.

[0016] In the figure: 1. Device housing; 2. Oscillation assembly; 21. Oscillation mechanism; 22. Oscillation rod; 3. Water bath tank; 4. Splash-proof assembly; 41. Support seat; 42. Upper cover; 43. Support block; 44. Sealing mechanism; 441. Mounting plate; 442. Sealing sleeve; 443. Sealing plate; 45. Connecting shaft; 5. Clamping assembly; 51. Connecting rod; 52. Clamping seat; 53. First clamping mechanism; 531. First clamping belt; 532. Fixed seat; 533. First spring; 534. Limiting rod; 535. Connecting belt; 536. Fixed block; 537. Clamping seat; 538. Movable plate; 54. Second clamping mechanism; 541. Second clamping belt; 542. Limiting seat; 543. Connecting block; 544. Wedge-shaped push block; 545. Block; 546. Second spring; 547. Movable seat; 55. Mounting mechanism; 551. Mounting seat; 552. Driving shaft; 553. Bi-directional lead screw; 554. Movable block; 56. Buffer mechanism; 561. Sealing seat; 562. Support sleeve; 563. Third spring; 564. Piston. Specific embodiments

[0017] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0018] Please refer to Figures 1 to 16 as shown, the present invention provides an embodiment: A cell culture device adaptable to culture dishes of various diameters, comprising a device housing 1, an oscillation assembly 2 and a water bath tank 3. The oscillation assembly 2 includes an oscillation mechanism 21 and an oscillation rod 22. The oscillation rod 22 is fixedly connected to the output end of the oscillation mechanism 21. A clamping assembly 5 is arranged inside the device housing 1. The clamping assembly 5 includes a connecting rod 51 fixedly connected to the oscillation rod 22. A clamping seat 52 is installed between the connecting rods 51 through an installation mechanism 55. A clamping mechanism one 53 and a clamping mechanism two 54 are arranged inside the clamping seat 52. Buffer mechanisms 56 are symmetrically arranged between the device housing 1 and the water bath tank 3; The clamping mechanism one 53 includes a clamping belt one 531, a fixed seat 532, a spring one 533, a connecting belt 535, a fixed block 536, a clamping seat 537 and a movable plate 538. The clamping seats 537 are symmetrically and fixedly connected to the outer sides of both ends of the clamping belt one 531. The spring one 533 is fixedly connected between the movable plate 538 and the fixed seat 532. The connecting belts 535 are symmetrically and fixedly connected between the movable plate 538 and the clamping seats 537. The fixed block 536 is used for clamping and fixing the connecting belts 535; A plurality of limiting rods 534 are evenly and fixedly connected between the fixed seat 532 and the clamping seat 52. The movable plate 538 is slidably connected to the outer sides of the limiting rods 534. A circular groove adapted to the connecting belt 535 is formed on the outer side of the fixed block 536; The clamping mechanism two 54 includes a clamping belt two 541, a limiting seat 542, a connecting block 543, a wedge-shaped push block 544, a clamping block 545, a spring two 546 and a movable seat 547. The clamping belt two 541 is slidably connected to the inside of the movable seat 547 through the clamping blocks 545. The spring two 546 is fixedly connected between the movable seats 547 and between the movable seat 547 and the inner wall of the limiting seat 542. The connecting block 543 and the wedge-shaped push block 544 are used for clamping and fixing the movable seat 547. The clamping blocks 545 are symmetrically and fixedly connected to both ends of the clamping belt two 541. A chute is formed inside the movable seat 547. The clamping mechanism two 54 is slidably connected to the inside of the chute. Card slots are symmetrically formed on the outer side of the movable seat 547. The connecting block 543 is slidably connected to the inside of the card slots. The wedge-shaped push block 544 is fixedly connected to the connecting block 543. The wedge-shaped push block 544 penetrates into the chute inside the movable seat 547. The connecting block 543 is fixedly connected to the fixed block 536.

[0019] In actual application of this embodiment, as Figure 1 、 Figure 2 、 Figure 3 and Figure 4 shown, the oscillation mechanism 21 transmits mechanical vibration to the clamping assembly 5 through the oscillation rod 22, driving the culture dish to perform a composite oscillation movement in three-dimensional space; As Figure 6 、 Figure 8 、 Figure 9 、 Figure 13 、 Figure 14, Figure 15 and Figure 16 As shown, the preliminary clamping when the petri dish is placed: When the petri dish is placed in the clamping seat 52, the petri dish first comes into contact with the first clamping belt 531 and the second clamping belt 541. At this time, the first clamping belt 531 undergoes a tensile deformation under the elastic action of the first spring 533 (the outer wall of the petri dish squeezes the first clamping belt 531, causing the first clamping belt 531 to drive the clamping seats 537 at both ends. The clamping seats 537 pull the movable plate 538 through the connecting belt 535, causing the movable plate 538 to slide outside the limiting rod 534 and compress the first spring 533). At the same time, the second clamping belt 541 also undergoes a tensile deformation under the elastic action of the second spring 546 (under the extrusion of the outer wall of the petri dish, the second clamping belt 541 drives the movable seat 547 to slide inside the limiting seat 542 through the clamping block 545 and compresses the second springs 546 at both ends). The two cooperate with each other to preliminarily clamp the four sides of the outside of the petri dish, so that the petri dish is preliminarily positioned in the device; As Figure 6 and Figures 8 to 16 shown, further clamping and fixing: After the preliminary clamping is completed, by operating the connecting block 543, the fixed block 536 fixedly connected to the connecting block 543 will move accordingly, causing it to downwardly stretch the connecting belt 535. Since the connecting belt 535 is symmetrically fixedly connected between the movable plate 538 and the clamping seat 537, the stretching of the connecting belt 535 will drive the movable plate 538 to slide outside the limiting rod 534, and at the same time cause the clamping seats 537 to move towards each other, thereby driving the two ends of the first clamping belt 531 to tighten, further clamping the petri dish; At the same time, the connecting block 543 drives the wedge-shaped push block 544 fixedly connected thereto to move. The wedge-shaped push block 544 penetrates into the internal sliding groove of the movable seat 547 and pushes the clamping block 545 to move. Since the clamping blocks 545 are symmetrically fixedly connected to both ends of the second clamping belt 541, the movement of the clamping blocks 545 causes the two ends of the second clamping belt 541 to tighten, achieving further clamping and fixing of the petri dish; So that through the way of elastic deformation and mechanical linkage, the device can adapt to petri dishes of different diameters, and ensure the clamping stability of the petri dish during oscillation, avoiding the shaking of the petri dish from affecting the cell culture environment; In addition, as Figures 2 to 4 shown, when there are too many petri dishes or the specifications exceed the threshold, the liquid level in the water bath tank 3 rises, resulting in an increase in the static pressure at the bottom. The pressure causes the liquid level to fall back through the buffer assembly 56 and reduces the liquid reflux, effectively suppressing the liquid oscillation and splashing.

[0020] As Figure 2 , Figure 3 and Figure 5As shown in the figure, a splash-proof component 4 is provided at the top of the device housing 1. The splash-proof component 4 includes a support base 41 and an upper cover 42. A support block 43 is fixedly connected to the top of the support base 41. Connecting shafts 45 are symmetrically and fixedly connected inside the upper cover 42. The upper cover 42 is rotatably connected to the top of the support block 43 through the connecting shafts 45. The connecting rod 51 passes through the support base 41 through a sealing mechanism 44.

[0021] In actual application of this embodiment, the support base 41 is fixedly connected to the top surface of the device housing 1. The support block 43 provided on its top is hinged to the upper cover 42 through the connecting shafts 45, so as to facilitate the rotation and opening of the upper cover 42. When the upper cover 42 is closed, it forms a surface contact seal with the support base 41, and together with the dynamic sealing mechanism 44 at the penetration of the connecting rod 51, leakage prevention and protection are achieved.

[0022] As Figure 2 and Figure 9 shown, the mounting mechanism 55 includes a mounting seat 551 fixedly connected to the bottom of the connecting rod 51. The mounting seat 551 is slidably connected to the outside of the clamping seat 52. A driving shaft 552 is rotatably connected inside the connecting rod 51. A bidirectional lead screw 553 is fixedly connected to the outside of the driving shaft 552. Movable blocks 554 are symmetrically and slidably connected inside the mounting seat 551. The two movable blocks 554 are symmetrically threadedly connected to the outside of the bidirectional lead screw 553. The connecting block 543 is fixedly connected to the movable block 554.

[0023] In actual application of this embodiment, it is mainly used to realize the connection between the connecting rod 51 and the clamping seat 52 and the driving of the second clamping mechanism 54 to adapt to the installation and stable clamping of culture dishes with different diameters. When it is necessary to install a culture dish and adjust the clamping mechanism to adapt to the diameter of the culture dish, the operator can rotate the driving shaft 552. Since the bidirectional lead screw 553 is fixedly connected to the outside of the driving shaft 552, the rotation of the driving shaft 552 will drive the bidirectional lead screw 553 to rotate synchronously. The movable blocks 554 symmetrically and slidably connected inside the mounting seat 551 are symmetrically threadedly connected to the bidirectional lead screw 553. According to the thread characteristics of the bidirectional lead screw 553, when the bidirectional lead screw 553 rotates, the two movable blocks 554 will move linearly in the mounting seat 551 along the axial direction of the lead screw in an approaching or separating manner. The connecting block 543 is fixedly connected to the movable block 554, and the movement of the movable block 554 will be transmitted to the connecting block 543. The connecting block 543 drives the wedge-shaped push block 544 connected thereto to move. The wedge-shaped push block 544 pushes the block 545, thereby tightening or loosening the two ends of the second clamping belt 541, realizing the further clamping or loosening operation of the culture dish, and ensuring that the culture dish is stably clamped in the clamping mechanism.

[0024] As Figures 2 to 4As shown in the figure, the buffer mechanism 56 includes a sealing seat 561 fixedly connected between the device housing 1 and the water bath tank 3. A piston 564 is slidably connected inside the sealing seat 561. On one side of the piston 564 close to the inner wall of the device housing 1, support sleeves 562 are symmetrically and fixedly connected. A third spring 563 is fixedly connected between the support sleeves 562 and the inner wall of the device housing 1. The bottom of the water bath tank 3 is uniformly provided with tapered holes that are larger on the inside and smaller on the outside.

[0025] In practical application of this embodiment, when more culture dishes are placed or the diameter of the culture dishes is too large, the liquid level in the water bath tank 3 will rise. Due to the increase in the liquid depth, the liquid pressure received at the bottom of the water bath tank 3 will increase. The bottom of the water bath tank 3 is uniformly provided with tapered holes that are larger on the inside and smaller on the outside. The increased pressure will be transmitted to the inside of the sealing seat 561 through these tapered holes. A piston 564 is slidably connected inside the sealing seat 561. The transmitted pressure will push the piston 564 to move towards the inner wall of the device housing 1. On one side of the piston 564 close to the inner wall of the device housing 1, support sleeves 562 are symmetrically and fixedly connected. A third spring 563 is fixedly connected between the support sleeves 562 and the inner wall of the device housing 1. When the piston 564 moves, the third spring 563 will be compressed, and the third spring 563 will generate an elastic reaction force. During the process of the pressure pushing the piston 564 to move, a part of the liquid flows into the space formed by the sealing seat 561 and the piston 564 through the tapered holes, causing the liquid level in the water bath tank 3 to drop, thereby alleviating the problem of excessive liquid level caused by the culture dish factor. At the same time, after the pressure decreases, the elastic reaction force of the third spring 563 will push the piston 564 to move in the reverse direction, enabling the liquid to flow back into the water bath tank 3 at an appropriate time to maintain the relative stability of the liquid level.

[0026] As Figure 2 、 Figure 3 、 Figure 5 、 Figure 6 and Figure 7 As shown in the figure, the sealing mechanism 44 includes a mounting plate 441 and a sealing sleeve 442. The mounting plate 441 is fixedly connected to the sealing sleeve 442. The sealing sleeve 442 is slidably connected inside the support seat 41. A sealing plate 443 is slidably connected inside the sealing sleeve 442. The sealing plate 443 is fixedly connected to the top of the connecting rod 51.

[0027] In practical application of this embodiment, when the connecting rod 51 moves with the oscillation assembly, the sealing plate 443 fixed to its top forms a linear sliding pair inside the sealing sleeve 442, and dynamic sealing is achieved through the precisely matched sliding surfaces.

[0028] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "comprising a..." does not exclude the presence of additional identical elements in the process, method, article or device comprising the said element.

[0029] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture device that can adapt to culture dishes of various diameters, comprising a device housing (1), an oscillating assembly (2) and a water bath (3), wherein the oscillating assembly (2) comprises an oscillating mechanism (21) and an oscillating rod (22), wherein the oscillating rod (22) is fixedly connected to an output end of the oscillating mechanism (21), and characterized in that: A clamping assembly (5) is arranged inside the device housing (1), the clamping assembly (5) comprising a connecting rod (51) fixedly connected to the oscillating rod (22), a clamping seat (52) is installed between the connecting rods (51) via a mounting mechanism (55), a clamping mechanism 1 (53) and a clamping mechanism 2 (54) are arranged inside the clamping seat (52), and a buffer mechanism (56) is symmetrically arranged between the device housing (1) and the water bath (3); The clamping mechanism (53) comprises a clamping belt (531), a fixing seat (532), a spring (533), a connecting belt (535), a fixing block (536), a clamping seat (537) and a movable plate (538); the clamping seat (537) is symmetrically fixedly connected to the outer sides of both ends of the clamping belt (531); the spring (533) is fixedly connected between the movable plate (538) and the fixing seat (532); the connecting belt (535) is symmetrically fixedly connected between the movable plate (538) and the clamping seat (537); and the fixing block (536) is used for clamping and fixing the connecting belt (535); The second clamping mechanism (54) comprises a second clamping belt (541), a limiting seat (542), a connecting block (543), a wedge-shaped push block (544), a clamping block (545), a second spring (546) and a movable seat (547); the second clamping belt (541) is slidably connected to the inner side of the movable seat (547) via the clamping block (545); the second spring (546) is fixedly connected between the movable seats (547) and between the movable seat (547) and the inner wall of the limiting seat (542); the connecting block (543) and the wedge-shaped push block (544) are used for clamping and fixing the movable seat (547).

2. The cell culture device that can adapt to culture dishes of various diameters according to claim 1, characterized in that: A splash-proof assembly (4) is arranged on the top of the device housing (1), and the splash-proof assembly (4) comprises a support seat (41) and an upper cover (42), the top of the support seat (41) is fixedly connected to a support block (43), the interior of the upper cover (42) is symmetrically fixedly connected to a connecting shaft (45), the upper cover (42) is rotatably connected to the top of the support block (43) via the connecting shaft (45), and the connecting rod (51) passes through the support seat (41) via a sealing mechanism (44).

3. The cell culture device that can adapt to culture dishes of various diameters according to claim 2, characterized in that: A limiting rod (534) is evenly and fixedly connected between the fixing seat (532) and the clamping seat (52), the movable plate (538) is slidably connected to the outer side of the limiting rod (534), and a circular groove matching the connecting belt (535) is formed on the outer side of the fixing block (536).

4. The cell culture device that can adapt to culture dishes of various diameters according to claim 2, characterized in that: The clamping block (545) is symmetrically fixedly connected to the two ends of the clamping belt (541); a sliding groove is provided inside the movable seat (547); the clamping mechanism (54) is slidably connected inside the sliding groove; a clamping groove is symmetrically provided on the outer side of the movable seat (547); the connecting block (543) is slidably connected inside the clamping groove; the wedge-shaped push block (544) is fixedly connected to the connecting block (543); the wedge-shaped push block (544) penetrates into the sliding groove inside the movable seat (547); and the connecting block (543) is fixedly connected to the fixed block (536).

5. The cell culture device that can be adapted to culture dishes of various diameters according to claim 2, characterized in that: The mounting mechanism (55) comprises a mounting seat (551) fixedly connected to the bottom of the connecting rod (51); the mounting seat (551) is slidably connected to the outer side of the clamping seat (52); the interior of the connecting rod (51) is rotatably connected to a driving shaft (552); the outer side of the driving shaft (552) is fixedly connected to a bidirectional screw rod (553); the interior of the mounting seat (551) is symmetrically slidably connected to a movable block (554); two movable blocks (554) are symmetrically threadedly connected to the outer side of the bidirectional screw rod (553); and the connecting block (543) is fixedly connected to the movable block (554).

6. The cell culture device that can adapt to culture dishes of various diameters according to claim 2, characterized in that: The buffer mechanism (56) comprises a sealing seat (561) fixedly connected between the device housing (1) and the water bath (3); a piston (564) is slidably connected inside the sealing seat (561); a support sleeve (562) is symmetrically fixedly connected to one side of the piston (564) close to the inner wall of the device housing (1); a spring three (563) is fixedly connected between the support sleeve (562) and the inner wall of the device housing (1); and a conical hole with a larger inner hole and a smaller outer hole is evenly opened at the bottom of the water bath (3).

7. The cell culture device that can be adapted to culture dishes of various diameters according to claim 3, characterized in that: The sealing mechanism (44) comprises a mounting plate (441) and a sealing sleeve (442); the mounting plate (441) is fixedly connected to the sealing sleeve (442); the sealing sleeve (442) is slidably connected to the interior of the support seat (41); a sealing plate (443) is slidably connected to the interior of the sealing sleeve (442); and the sealing plate (443) is fixedly connected to the top of the connecting rod (51).

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