Method for calculating content range of mangiferin in mango seeds and application

Abstract

The invention discloses a method for calculating the content range of mangiferin in mango kernels and application. The method comprises the following steps: randomly sampling the mango kernels from batches of to-be-detected mango kernels, breaking shells of the mango kernels, taking out internal seeds, grouping the shells and the seeds of the mango kernels, and calculating the weight of the shells and the weight of the seeds in each group; respectively pulverizing and sieving the shells and the seeds according to groups, detecting the mangiferin content in each group of shells and the mangiferin content in the seeds, and calculating the theoretical mangiferin content of each group of mango kernels; and calculating the mangiferin content range in the to-be-detected batch of mango kernels according to the theoretical mangiferin content of each group of mango kernels. The method can accurately calculate the range of the mangiferin content in the mango seeds, can accurately judge whether the content of the batch of medicinal materials meets the internal control standard or not by comparing whether the lower limit value of the content range is higher than the internal control standard requirement of the medicinal materials or not, avoids the situation that the medicinal materials with unqualified content are misjudged to be qualified, and reduces the risk that the medicinal materials are qualified.

Description

Technical Field

[0001] The present invention belongs to the field of mangiferin detection, and particularly relates to a method for calculating the content range of mangiferin in mango kernels and its application. Background Art

[0002] Mango kernel is the dried seed with endocarp of the plant Mangifera indica L. of Anacardiaceae (commonly known as fruit kernel), which has the effect of clearing heat and relieving stagnation, and is used for internal accumulation, stagnation, hernia, etc. The chemical constituents of mango kernel include flavonoids, organic acids, coumarins, etc. Mangiferin is a natural glucosylxanthone found in mangoes, which has pharmacological activities such as liver protection, anti-cancer, anti-diabetes, and antiviral against herpes simplex virus and poliovirus.

[0003] The shell of mango kernel is hard and has a lot of fibers; the contained seed has a thin seed coat and thick seed kernel. The overall content level of mangiferin in mango kernel is relatively low, less than 0.1%, and the mangiferin content in the seed is significantly higher than that in the shell. When detecting the mangiferin content in traditional Chinese medicine mango kernel, there is a problem of poor accuracy of the detection results. Sampling problems and preparation problems of test samples are important factors leading to detection errors. For example, during the process of powdering and sieving the medicinal materials in the pretreatment of test samples, the shell powder fibers of mango kernel are easily adhered to the inner wall of the powdering machine, and it is difficult to pass through the sieve and easy to adhere to the sieve. While the seed powder adheres less and is easy to pass through the sieve. Therefore, the loss of shell powder is greater than that of seed powder during the powdering and sieving process, and the loss ratio each time is uncertain. Because the mangiferin content in mango kernel seeds is significantly higher than that in the shell, it will cause the final detected content result of the sample to be easily on the high side, and the results vary greatly each time, which causes great interference to the judgment of whether the mangiferin content in traditional Chinese medicine mango kernel is qualified. Summary of the Invention

[0004] Object of the Invention: The object of the present invention is to provide a method for calculating the content range of mangiferin in mango kernel to improve the accuracy of detection results; another object of the present invention is to provide the application of this method, which can be used to judge whether the content of mango kernel medicinal materials is qualified, avoid the mixing of low-content inferior medicinal materials, and greatly reduce the risk of medicinal material release.

[0005] Technical Solution: The method for calculating the content range of mangiferin in mango kernel according to the present invention includes the following steps:

[0006] (1) Randomly sample n mango kernels from the mango kernels of the batch to be inspected, where n is 20 - 50;

[0007] (2) Test sample detection: Break the outer shell of the mango kernels sampled in step (1), take out the internal seeds, weigh the weight of the outer shell and the seeds one by one. After grouping the weighed mango kernel outer shells and seeds, calculate the weight of the outer shell and the seeds in each group; powder and sieve the above-mentioned outer shells and seeds by group, detect the content of mangiferin in the outer shell of each group and the content of mangiferin in the seeds, and calculate the theoretical mangiferin content of each group of mango kernels;

[0008] (3) Content range calculation: Calculate the mangiferin content range in the mango kernels of the batch to be tested according to the theoretical mangiferin content of each group of mango kernels in step (2).

[0009] The incoming quantity of a batch of mango kernel medicinal materials is generally more than 1 kg under normal circumstances (the actual medicinal material purchase quantity is often counted in hundreds of kilograms). The weight of a single mango kernel is generally about 10 - 20 g, and the total weight of 20 mango kernels will not exceed 0.5 kg. The total amount of the incoming mango kernel medicinal materials far exceeds 0.5 kg, so the situation of sampling less than 20 mango kernels will not occur.

[0010] Preferably, the specific detection method in step (2) is: Randomly sort and number the n sampled mango kernels as 1, 2, 3... n, break the outer shells respectively, take out the internal seeds, and weigh the weight of the outer shell (m i1 ) and the weight of the seeds (m i2 ), i = 1, 2,... n; Group the weighed mango kernel outer shells and seeds in groups of t in sequence, where t is 3 - 8, and there are n / t groups for both the outer shell and the seed samples; t can be flexibly adjusted within the range of 3 - 8 according to the actual sampling quantity n to ensure that n mango kernels can be evenly divided into n / t groups, and n / t is an integer; Calculate the weight of the outer shell (M j1 ) and the weight of the seeds (M j2 ) in each group, j = 1, 2,... n / t; Powder and sieve the above-mentioned outer shells and seeds by group respectively to prepare the test solution, and detect the content of mangiferin in the outer shell of each group (C j1 ) and the content of mangiferin in the seeds (C j2 ), then the theoretical mangiferin content of each group of mango kernels is:

[0011] C j = (C j1 × M j1 + C j2 × M j2 ) / (M j1 + M j2 ) (j = 1, 2,... n / t).

[0012] Preferably, the high performance liquid chromatography method is used to detect the content of mangiferin in the outer shell of each group (C j1 ) and the content of mangiferin in the seeds (C j2 ).

[0013] Preferably, the specific calculation method for the content range H = [A, B] of mangiferin in the mango kernels of the batch to be tested in step (3) is as follows:

[0014]

[0015] Preferably, in step (1), the specific sampling includes package sampling, in-package sampling, and test sample preparation.

[0016] Preferably, the package sampling step includes extracting samples for inspection from the packages of mango kernels of the batch to be tested. If the number of batch packages is less than 3, sample each package one by one; if the number of batch packages is greater than 3, the number of samples to be taken is When it cannot be divided evenly, round up or down to an integer.

[0017] Preferably, the in-package sampling step includes: if the package quantity is less than 5 kg, sample at 10%, and the net mass of each portion is not less than 100 g; if the package quantity is greater than 5 kg, take 3 samples from the upper, middle, and lower positions, with the net mass of each portion being 240 - 260 g, for a total of 720 - 780 g.

[0018] Preferably, the test sample preparation step includes mixing all the above-extracted samples, spreading them out into a square, drawing a "×" along the diagonal to divide them into four equal parts, and taking two diagonal parts; then operating as above and repeating several times until the final remaining number of mango kernels is n, where n is 20 - 50. When n is 23, 29, 31, 37, 41, 43, or 47, randomly remove one mango kernel so that n satisfies being 20 - 50 and can be divided evenly by t, where t is at least one of 3 - 8.

[0019] Application of the method for calculating the content range of mangiferin in mango kernels according to the present invention.

[0020] Preferably, it is applicable to the detection and analysis of the content range of mangiferin in mango kernels of different varieties, origins, and batches.

[0021] Preferably, it is used to judge whether the mangiferin content meets the internal control standards of the medicinal materials.

[0022] Beneficial effects: Compared with the prior art, the present invention has the following remarkable advantages: The method of the present invention can accurately calculate the range of the content of mangiferin in mango kernels, and the true value of the content of mangiferin in this batch of mango kernels is within this range, which can be used to judge whether the content of mangiferin meets the internal control standards of the medicinal materials. Compared with the general detection method which is prone to result in a higher detection result of the mangiferin content in mango kernels and is difficult to determine the true value of the mangiferin content of the whole batch of medicinal materials, it can accurately judge whether the content of this batch of medicinal materials meets the internal control standards by comparing whether the lower limit value of the content range is higher than the requirements of the internal control standards of its own medicinal materials, avoiding misjudging unqualified medicinal materials as qualified and reducing the risk of qualified release of medicinal materials. At the same time, the method of the present invention strengthens the control of the sampling link of mango kernel medicinal materials, reasonably extracts the original samples, and standardizes the implementation of sample merging, sub-sampling and other processing operations, further improving the accuracy of the detection results. Detailed implementation manners

[0023] The technical solutions of the present invention will be further described below in conjunction with the embodiments.

[0024] Example 1

[0025] This example involves the calculation of the range of the content of mangiferin in a batch of mango kernel medicinal materials (batch number: 2104771, origin: Yulin, Guangxi). The total amount of this batch of medicinal materials is 1000 kg, the number of packages is 25, and the packaging amount per piece is 40 kg.

[0026] (1) Sampling from packages: Randomly select pieces from 25 packages of mango kernels;

[0027] (2) Sampling within packages: From the above 6 packages of mango kernels, take 3 samples from the upper, middle and lower parts respectively, each with a net weight of 250 g, for a total of 4500 (=250×3×6) g;

[0028] (3) Preparation of test samples: Mix the above-mentioned 4500 g of mango kernel samples evenly, spread them into a square, draw a "×" along the diagonal to divide them into four equal parts, and take two diagonal parts; repeat the operation 4 times until the final remaining total weight of mango kernels is 252.1 g and the number of mango kernels is 20.

[0029] (4) Detection of test samples: Randomly number the above 20 sampled mango kernels as 1, 2, 3... 20, break the outer shells in sequence, take out the internal seeds, and weigh the outer shell weight m i1 (5.072.27 4.28 3.69 3.25.24 3.33.322.72 3.18 3.09 3.27 3.64.33 5.26 4.75 3.83 4.19 3.113.93) and the seed weight m i2(10.135.43 10.22 9.51 8.58.16 8.77.88 7.48 9.52 7.61 10.13 8.28.57 10.24 12.45 8.677.31 8.69 9.07). After weighing, the mango kernel shells and seeds are respectively grouped in sets of 4 in sequence. There are 5 sets each for the shell and seed samples, and the weight of each set of shells is denoted as M j1 (3.83 3.77 3.07 4.49 3.77) and the weight of the seeds is M j2 (8.82 8.318.69 9.87 8.44). The above-mentioned shells and seeds are respectively ground and sieved by group, and the mangiferin content C in each set of shells is detected j1 (0.00330 0.00234 0.00145 0.00134 0.00175) and the mangiferin content C in the seeds j2 (0.040060.01164 0.05463 0.08097 0.01568). Then the theoretical mangiferin content of each set of mango kernels is (0.0289 0.0087 0.0408 0.0561 0.0114) (5) Calculate the content range: Calculate the mangiferin content range H = [A, B] of this batch of mango kernels

[0036]

[0037] That is, the mangiferin content range of this batch of mango kernels is 0.00761% - 0.0620%.

[0038] Continue to repeat the above sampling, detection and calculation process for the mango kernel medicinal materials of this batch twice, and summarize the mangiferin content ranges obtained from the 3 calculations. The results are as follows:

[0039]

[0040] Comparative Example 1

[0041] This example involves the determination of the mangiferin content in the mango kernel medicinal materials of the same batch as the example. The difference is that after sampling according to the sampling principle under Part IV of the Chinese Pharmacopoeia (2020 Edition) (Sampling Method for Medicinal Materials and Slices, General Rule 0211), the content of the medicinal materials is determined by the general method. The shells and seeds are not separated, ground and sieved together to prepare the test solution, and the mangiferin content is detected by high performance liquid chromatography. A total of 5 detections are carried out, and the detection results are as follows:

[0042]

[0043] The content range of mangiferin in the five test results of this batch of medicinal materials is 0.0099-0.0629%, and the RSD is as high as 70.34%, making it difficult to evaluate the true level of mangiferin content in this batch of medicinal materials.

Claims

1. A method for calculating the content range of mangiferin in mango kernels, characterized in that: The steps include: (1) Randomly sample n mango pits from the batch of mango pits to be tested, where n is 20 to 50; (2) Testing of test samples: breaking the shell of the mango kernel sampled in step (1), taking out the seeds inside, weighing the shell weight and the seed weight one by one, grouping the weighed mango kernel shells and seeds, and calculating the shell weight and seed weight of each group; pulverizing and sieving the shells and seeds for each group, detecting the mangiferin content in the shell of each group and the mangiferin content in the seeds, and calculating the theoretical mangiferin content of each group of mango kernels; (3) Calculation of content range: Calculate the content range of mangiferin in the mango kernels of the batch to be tested based on the theoretical mangiferin content of each group of mango kernels in step (2).

2. The method for calculating the content range of mangiferin in mango kernel according to claim 1, characterized in that: The specific detection method of step (2) is as follows: randomly order the n mango pits sampled above and number them 1, 2, 3, ... n, break the shells respectively, take out the seeds inside, and weigh the shells one by one (m i1 ) and seed weight (m i2 ), i = 1, 2, ... n; the weighed mango core shells and seeds are grouped in order of t pieces, where t is 3 to 8, and the shell and seed samples are n / t groups respectively; t can be flexibly adjusted in the range of 3 to 8 according to the actual sampling amount n to ensure that n mango cores can be evenly divided into n / t groups, where n / t is an integer; the weight of each group of shells (M j1 ) and seed weight (M j2 ), j = 1, 2, ... n / t; the shells and seeds were powdered and sieved according to the corresponding groups to prepare the test solution, and the mangiferin content in each group of shells was detected (C j1 ) and mangiferin content in seeds (C j2 ), then the theoretical mangiferin content of each group of mango kernels is:

3. The method for calculating the content range of mangiferin in mango kernel according to claim 2, characterized in that: The specific calculation method of the mangiferin content range H = [A, B] in the mango kernel of the batch to be tested in step (3) is:

4. The method for calculating the content range of mangiferin in mango kernel according to claim 1, characterized in that: In step (1), specific sampling includes package sampling, sampling inside the package and test sample preparation.

5. The method for calculating the content range of mangiferin in mango kernel according to claim 4, characterized in that: The package sampling step includes extracting samples for inspection from the mango pit packages of the batch to be inspected. If the number of packages in the batch is less than 3, samples are taken piece by piece; if the number of packages in the batch is greater than 3, samples are taken piece by piece. If the divisibility is not exact, round it up.

6. The method for calculating the content range of mangiferin in mango kernel according to claim 4, characterized in that: The sampling steps in the package include: if the packaging volume is less than 5kg, sampling is carried out at 10%, and the net weight of each sample is not less than 100g; if the packaging volume is greater than 5kg, 3 samples are taken from the top, middle and bottom positions, and the net weight of each sample is 240-260g, totaling 720-780g.

7. The method for calculating the content range of mangiferin in mango kernel according to claim 4, characterized in that: The test sample preparation step comprises mixing all the samples extracted above, spreading them into a square, drawing an "×" along the diagonal line to divide them into four equal parts, and taking two diagonal parts; and repeating the above operation several times until the number of mango cores remaining is n, where n is 20 to 50.

8. An application of the method for calculating the content range of mangiferin in mango kernel according to claim 1.

9. The use according to claim 8, characterized in that: It is suitable for the detection and analysis of the mangiferin content range in mango kernels of different varieties, origins and batches.

10. The use according to claim 8, characterized in that: Used to determine whether the mangiferin content meets the internal control standards of medicinal materials.

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