Biological cell culture device for ophthalmology department

Abstract

The invention belongs to the technical field of microorganisms, and discloses an ophthalmic biological cell culture device which comprises a culture medium cover assembly, a liquid homogenizing assembly and a liquid adding valve assembly are arranged at the top end of the culture medium cover assembly, and the liquid adding valve assembly forms a self-closing and pressing self-opening culture solution adding structure at the top end of the culture medium cover assembly. The liquid homogenizing assembly forms a culture solution homogenizing structure which rotates circularly along with rotation and rotates secondarily at the top end of the culture medium cover assembly, during circular motion, a liquid homogenizing arc tube at the bottom of a liquid passing straight tube scatters culture solution or other biological agent liquid onto ophthalmology culture cell tissues in a circular motion mode, during secondary rotation of circular motion, rotation of the liquid homogenizing arc tube during circular motion is achieved through rotation, and the culture solution or other biological agent liquid can be dispersed onto the ophthalmology culture cell tissues in a circular motion mode. At the moment, the culture solution or other biological agent liquid can be more uniformly scattered on ophthalmology culture cell tissues, and meanwhile, the uniform scattering of the culture solution or other biological agent liquid is also accelerated.

Description

Technical Field

[0001] The present invention belongs to the technical field of microbiology, and particularly relates to an ophthalmic biological cell culture device. Background Art

[0002] Microbial cell culture refers to obtaining a large number of cells or their metabolites through cell culture. Since biological products are all derived from cells, it can be said that cell culture technology is the most core and fundamental technology in biotechnology.

[0003] The prior art has the following problems: Ophthalmic biological cell culture is the most basic technology in ophthalmic biotechnology. When performing ophthalmic biological cell culture, culture media, etc. are required. When using the existing culture media, the extracted tissue is placed in a base box, culture solution is added, and then the base cover is covered. In the subsequent process, there are additions of culture solution again, muscle enzyme solution, subsequent staining solution and fixing solution, etc. The prior art has the following problems. Each addition of culture solution or other biological solution requires opening or uncovering the base cover, which causes bacteria to easily invade the ophthalmic cultured cell tissue. At the same time, when adding the existing culture solution or other biological solution, the culture solution or other biological solution cannot be well scattered evenly on the ophthalmic cultured cell tissue, resulting in a long subsequent liquid homogenization time or increased difficulty in liquid homogenization. Therefore, it is urgent to solve the above problems. Summary of the Invention

[0004] To solve the problems raised in the above background art, the present invention provides an ophthalmic biological cell culture device, which has the characteristics of convenient use.

[0005] To achieve the above object, the present invention provides the following technical solution: An ophthalmic biological cell culture device includes a culture medium cover assembly. A liquid homogenization assembly and a liquid adding valve assembly are provided at the top of the culture medium cover assembly, and a culture medium box is buckled below the culture medium cover assembly. The liquid adding valve assembly forms a self-closing and press-to-open culture solution adding structure at the top of the culture medium cover assembly. During the pressing and resetting process, the liquid homogenization assembly forms a culture solution liquid homogenization structure that follows the movement of a ring and rotates twice around the ring at the top of the culture medium cover assembly.

[0006] In a preferred embodiment of an ophthalmic biological cell culture device, the culture medium cover assembly includes an outer base cover. A drive gear ring and an edge gear are rotatably provided on the top of the outer base cover. A driven bevel gear is coaxially and fixedly provided on the top of the edge gear. Inner teeth and outer teeth are respectively fixedly provided on the inner side and the outer side of the drive gear ring. An L-shaped support arm is fixedly provided on the edge of the outer base cover. A ring bin is fixedly provided on the top of the L-shaped support arm. A spherical bin is fixedly provided on the inner side of the ring bin. A T-shaped bin and a vertical rod are fixedly provided on the bottom of the spherical bin. A bottom rod is fixedly provided on the bottom of the T-shaped bin. An arc-shaped ring bin is rotatably provided on the edge of the T-shaped bin through a bearing. A disinfected alcohol cotton pad is provided on the inner wall of the spherical bin. A liquid absorption cotton thread is provided on the outer side of the disinfected alcohol cotton pad. An alcohol bin is formed between the spherical bin and the ring bin. A liquid storage cavity is formed between the T-shaped bin and the arc-shaped ring bin. Perforated pedestals are fixedly provided on both sides of the bottom of the ring bin. An inner base cover is fixedly provided on the bottom of the bottom rod. A fixed gear ring is fixedly provided on the top of the inner base cover. A ring groove is provided between the tops of the inner base cover and the outer base cover; The liquid distribution assembly includes a liquid distribution ring platform. A plurality of liquid passing straight pipes are rotatably provided on the liquid distribution ring platform through bearings. A liquid distribution arc pipe is fixedly provided on the bottom of the liquid passing straight pipe. A liquid distribution gear is fixedly provided on the top of the liquid passing straight pipe. A liquid passing cap pipe is rotatably provided at the top end of the liquid passing straight pipe through a bearing. An inclined liquid passing inclined pipe is fixedly provided on one side of the liquid passing cap pipe; The liquid adding valve assembly includes a valve shaft rod and a drive end sleeve disc. A bevel gear and a drive shaft rod are fixedly provided at one end of the valve shaft rod. A liquid adding ball valve is fixedly provided at the other end of the valve shaft rod. A liquid adding groove is provided on the liquid adding ball valve. A return spring is sleeved outside the drive shaft rod. A drive convex head is fixedly provided on the inner wall of the drive end sleeve disc. Guide slide rods are fixedly provided on both sides of the drive end sleeve disc.

[0007] In a preferred embodiment of an ophthalmic biological cell culture device, the liquid distribution ring platform is rotatably provided through a bearing at the ring groove between the inner base cover and the outer base cover. The liquid distribution gear is arranged between the fixed gear ring and the drive gear ring. One side of the liquid distribution gear meshes with the outer side of the fixed gear ring, and the other side of the liquid distribution gear meshes with the inner teeth on the inner side of the drive gear ring.

[0008] In a preferred embodiment of an ophthalmic biological cell culture device, the inclined liquid passing inclined pipe is fixedly and penetratingly provided in the arc-shaped ring bin. Disinfected alcohol is filled in the alcohol bin. The disinfected alcohol cotton pad siphons the disinfected alcohol in the alcohol bin to the disinfected alcohol cotton pad through the liquid absorption cotton thread for moistening.

[0009] In a preferred embodiment of an ophthalmic biological cell culture device, the liquid adding ball valve rotates in the spherical bin. The tail end of the valve shaft rod is rotatably connected to the ring bin and the spherical bin through bearings. The drive shaft rod and the bevel gear are arranged outside the ring bin.

[0010] In a preferred embodiment of an ophthalmic biological cell culture device, the driving end sleeve disc is sleeved on the driving shaft rod, the driving convex head is inserted into the driving spiral chute, one end of the return spring abuts against the driving end sleeve disc, and the other end of the return spring abuts against the outer wall of the annular chamber.

[0011] In a preferred embodiment of an ophthalmic biological cell culture device, the bevel gear at one end of the valve shaft rod meshes with the driven bevel gear, and the edge gear meshes with the external teeth on the outer side of the driving gear ring.

[0012] In a preferred embodiment of an ophthalmic biological cell culture device, the driving end sleeve disc linearly reciprocates on the driving shaft rod, and the guiding slide rod linearly reciprocates in the perforated base table.

[0013] Compared with the prior art, the beneficial effects of the present invention are as follows: The liquid adding valve assembly forms a self-closing and pressing self-opening culture solution adding structure on the top of the culture medium cover assembly. During the pressing and resetting process, the liquid leveling assembly forms a culture solution leveling structure on the top of the culture medium cover assembly that follows the movement of the ring and rotates twice during the ring movement. When it is necessary to add the culture solution again, at this time, the liquid adding groove and the liquid storage cavity are directly opposite and communicated, that is, at this time, the culture solution can be added to the liquid storage cavity through the liquid adding groove or other biological agent liquids. At the same time, it should be noted that when the driving end sleeve disc is not pressed, at this time, the liquid adding groove on the liquid adding ball valve is misaligned with the liquid storage cavity, that is, the liquid adding ball valve seals the open top of the spherical chamber. In this way, self-closing is achieved, thus effectively ensuring the safety during ophthalmic biological cell culture. The liquid adding ball valve rotates in the spherical chamber, and a disinfected alcohol cotton pad is arranged on the inner wall of the spherical chamber. The disinfected alcohol cotton pad siphons the disinfected alcohol in the alcohol storage chamber to the disinfected alcohol cotton pad through the liquid absorbing cotton thread for wetting. When the liquid adding ball valve rotates in the spherical chamber, the disinfected alcohol cotton pad can repeatedly wipe and disinfect the port of the liquid adding groove and the sphere of the liquid adding ball valve in a rotating manner. In this way, the disinfection and sterility of the liquid adding port are further ensured. After the addition is completed, the liquid in the liquid storage cavity flows into the liquid guiding straight pipe through a plurality of inclined liquid guiding inclined pipes that are inclined downward and scatters from both ends of the liquid leveling arc pipe. During the reset sliding process, a plurality of liquid guiding straight pipes on the liquid leveling ring platform rotate around the outer base cover. On the basis of the rotation, the liquid guiding straight pipe rotates again for the second time. In this way, during the reset process, synchronous rotation around the ring and synchronous second rotation of a plurality of liquid guiding straight pipes on the top of the outer base cover are achieved. During the rotation around the ring and the second rotation, during the rotation around the ring, the liquid leveling arc pipe at the bottom of the liquid guiding straight pipe scatters the culture solution or other biological agent liquids around the ophthalmic culture cell tissue in a circular motion. During the second rotation during the rotation around the ring, through the self-rotation, the self-rotation of the liquid leveling arc pipe during the rotation around the ring is achieved. At this time, the culture solution or other biological agent liquids can be scattered more evenly on the ophthalmic culture cell tissue, and at the same time, the uniform scattering of the culture solution or other biological agent liquids is accelerated. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1Isometric view of the present invention; Figure 2 Cross-sectional view of the present invention; Figure 3 Exploded view of the present invention; Figure 4 Isometric view of the culture medium cover assembly of the present invention; Figure 5 Cross-sectional view of the annular bin of the present invention; Figure 6 Isometric view of the liquid equalizing assembly of the present invention; Figure 7 Isometric view of the liquid adding valve assembly of the present invention; Figure 8 Exploded view of the liquid adding valve assembly of the present invention; In the figure: 100, culture medium cover assembly; 101, outer base cover; 102, annular groove; 103, edge gear; 104, driven bevel gear; 105, annular bin; 106, L-shaped support arm; 107, inner tooth; 108, outer tooth; 109, driving gear ring; 110, inner base cover; 111, fixed gear ring; 112, spherical bin; 113, disinfected alcohol cotton pad; 114, liquid absorbing cotton thread; 115, perforated base table; 116, arc-shaped annular bin; 117, liquid storage cavity; 118, I-shaped bin; 119, vertical rod; 120, bottom rod; 121, alcohol bin; 200, liquid equalizing assembly; 201, liquid equalizing ring table; 202, liquid passing straight pipe; 203, liquid equalizing arc pipe; 204, liquid equalizing gear; 205, liquid passing cap pipe; 206, inclined liquid passing inclined pipe; 300, liquid adding valve assembly; 301, valve shaft rod; 302, driving shaft rod; 303, driving end sleeve plate; 304, driving convex head; 305, guiding slide rod; 306, liquid adding groove; 307, liquid adding ball valve; 308, return spring; 309, driving spiral chute; 310, bevel gear; 400, culture medium box. Detailed implementation manners

[0015] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0016] Please refer to Figures 1-8As shown in the figure, the present invention provides an ophthalmic biological cell culture device, which includes a culture medium cover assembly 100. A liquid homogenizing assembly 200 and a liquid adding valve assembly 300 are arranged at the top of the culture medium cover assembly 100. And a culture medium box 400 is buckled below the culture medium cover assembly 100. The liquid adding valve assembly 300 forms a self-closing and press-to-open culture solution adding structure at the top of the culture medium cover assembly 100. During the pressing and resetting process, the liquid homogenizing assembly 200 forms a culture solution homogenizing structure that follows the movement of the ring and rotates twice around the ring at the top of the culture medium cover assembly 100.

[0017] In a preferred embodiment, please refer to Figure 4 and Figure 5 , the culture medium cover assembly 100 includes an outer base cover 101. A driving gear ring 109 and an edge gear 103 are rotatably arranged at the top of the outer base cover 101. A driven bevel gear 104 is coaxially and fixedly arranged at the top of the edge gear 103. Inner teeth 107 and outer teeth 108 are respectively fixedly arranged on the inner side and the outer side of the driving gear ring 109. An L-shaped support arm 106 is fixedly arranged at the edge of the outer base cover 101. A ring bin 105 is fixedly arranged at the top of the L-shaped support arm 106. A spherical bin 112 is fixedly arranged inside the ring bin 105. A I-shaped bin 118 and a vertical rod 119 are fixedly arranged at the bottom of the spherical bin 112. A bottom rod 120 is fixedly arranged at the bottom of the I-shaped bin 118. And an arc-shaped ring bin 116 is rotatably arranged at the edge of the I-shaped bin 118 through a bearing. A disinfected alcohol cotton pad 113 is arranged on the inner wall of the spherical bin 112. A liquid absorbing cotton thread 114 is arranged outside the disinfected alcohol cotton pad 113. An alcohol bin 121 is formed between the spherical bin 112 and the ring bin 105. A liquid storage cavity 117 is formed between the I-shaped bin 118 and the arc-shaped ring bin 116. Perforated seat platforms 115 are fixedly arranged on both sides at the bottom of the ring bin 105. An inner base cover 110 is fixedly arranged at the bottom of the bottom rod 120. A fixed gear ring 111 is fixedly arranged at the top of the inner base cover 110. A ring groove 102 is arranged between the top of the inner base cover 110 and the outer base cover 101. An inclined liquid passing inclined tube 206 is fixedly arranged through the arc-shaped ring bin 116. The alcohol bin 121 is filled with disinfected alcohol. The disinfected alcohol cotton pad 113 siphons the disinfected alcohol in the alcohol bin 121 to the disinfected alcohol cotton pad 113 through the liquid absorbing cotton thread 114 for wetting.

[0018] In a preferred embodiment, please refer to Figure 6, the liquid distribution component 200 includes a liquid distribution ring platform 201. A plurality of liquid passing straight pipes 202 are rotatably arranged on the liquid distribution ring platform 201 through bearings. A liquid distribution arc pipe 203 is fixedly arranged at the bottom of the liquid passing straight pipe 202, and a liquid distribution gear 204 is fixedly arranged at the top of the liquid passing straight pipe 202. A liquid passing cap pipe 205 is rotatably arranged at the top end of the liquid passing straight pipe 202 through a bearing. An inclined liquid passing inclined pipe 206 is fixedly arranged on one side of the liquid passing cap pipe 205. The liquid distribution ring platform 201 is rotatably arranged at the ring groove 102 between the inner base cover 110 and the outer base cover 101 through a bearing. The liquid distribution gear 204 is arranged between the fixed tooth ring 111 and the driving tooth ring 109. One side of the liquid distribution gear 204 is meshed with the outer side of the fixed tooth ring 111, and the other side of the liquid distribution gear 204 is meshed with the inner tooth teeth 107 on the inner side of the driving tooth ring 109.

[0019] In a preferred embodiment, please refer to Figure 7 and Figure 8 , the liquid adding valve component 300 includes a valve shaft rod 301 and a driving end sleeve disc 303. A conical gear 310 and a driving shaft rod 302 are fixedly arranged at one end of the valve shaft rod 301, and a liquid adding ball valve 307 is fixedly arranged at the other end of the valve shaft rod 301. A liquid adding groove 306 is formed on the liquid adding ball valve 307. A return spring 308 is sleeved outside the driving shaft rod 302. A driving convex head 304 is fixedly arranged on the inner wall of the driving end sleeve disc 303, and guide sliding rods 305 are fixedly arranged on both sides of the driving end sleeve disc 303. The liquid adding ball valve 307 rotates in the spherical chamber 112. The tail end of the valve shaft rod 301 is rotatably connected to the ring chamber 105 and the spherical chamber 112 through a bearing. The driving shaft rod 302 and the conical gear 310 are arranged outside the ring chamber 105. The driving end sleeve disc 303 is sleeved on the driving shaft rod 302. The driving convex head 304 is inserted into the driving spiral chute 309. One end of the return spring 308 abuts against the driving end sleeve disc 303, and the other end of the return spring 308 abuts against the outer wall of the ring chamber 105. The conical gear 310 at one end of the valve shaft rod 301 is meshed with the driven conical gear 104, and the edge gear 103 is meshed with the outer tooth teeth 108 on the outer side of the driving tooth ring 109. The driving end sleeve disc 303 linearly reciprocates on the driving shaft rod 302, and the guide sliding rods 305 linearly reciprocate in the perforated seat platform 115.

[0020] Generally speaking, when performing ophthalmic biological cell culture, a certain amount of culture medium and biological cells are added into the culture medium box 400, and then the culture medium cover assembly 100 is buckled and sealed on the top of the culture medium box 400 (in order to better isolate from the outside world and avoid contamination, in the prior art, plastic wrap is wound around the edges of the culture medium cover assembly 100 and the culture medium box 400). At this time, during the early, middle and late stages of ophthalmic biological cell culture, it may be necessary to add a certain amount of culture medium, muscle enzyme solution, staining solution, fixing solution, etc. again. The traditional technology will open the culture medium cover assembly 100 and the culture medium box 400, which greatly increases the exposed area of ophthalmic biological cell culture and increases the risk coefficient of contamination. However, the present invention makes technical improvements on the culture medium cover assembly 100. When it is necessary to add the culture medium again, at this time, press the driving end sleeve 303 backward, and the driving end sleeve 303 slides linearly towards the tail end of the driving shaft rod 302. During the linear sliding process, since the driving convex head 304 is inserted into the driving spiral chute 309, at this time, when the driving end sleeve 303 slides backward linearly, through the cooperation of the driving convex head 304 and the driving spiral chute 309, at this time, the liquid adding ball valve 307 rotates in the spherical chamber 112, that is, at this time, the liquid adding groove 306 on the liquid adding ball valve 307 rotates towards the opening at the top of the spherical chamber 112. At this time, the liquid adding groove 306 and the liquid storage cavity 117 are aligned and communicated. That is, at this time, the culture medium can be added into the liquid storage cavity 117 through the liquid adding groove 306 or other biological agent liquids. At the same time, it should be noted that when the driving end sleeve 303 is not pressed, at this time, the liquid adding groove 306 on the liquid adding ball valve 307 is misaligned with the liquid storage cavity 117, that is, the liquid adding ball valve 307 seals the opening at the top of the spherical chamber 112. Through this method, self-closure is realized, thereby effectively ensuring the safety during ophthalmic biological cell culture.

[0021] On the above basis, the liquid adding ball valve 307 rotates in the spherical chamber 112. A disinfected alcohol cotton pad 113 is arranged on the inner wall of the spherical chamber 112. The disinfected alcohol cotton pad 113 siphons the disinfected alcohol in the alcohol storage chamber 121 to the disinfected alcohol cotton pad 113 through the liquid absorbing cotton thread 114 for moistening. When the liquid adding ball valve 307 rotates in the spherical chamber 112, the disinfected alcohol cotton pad 113 can repeatedly wipe and disinfect the port of the liquid adding groove 306 and the sphere of the liquid adding ball valve 307 in a rotating manner. Through this method, the disinfection and sterility of the liquid adding port are further ensured.

[0022] On the basis described above, after the addition is completed, the liquid in the liquid storage cavity 117 flows into the liquid passing straight pipe 202 through a plurality of downwardly inclined liquid passing inclined pipes 206 and scatters from both ends of the liquid equalizing arc pipe 203. At this time, the pressing force on the driving end sleeve 303 is released. At this time, through the reset of the reset spring 308, the driving end sleeve 303 slides linearly from the tail end to the front end of the driving shaft rod 302. During the reset sliding process, through the cooperation of the driving convex head 304 and the driving spiral chute 309, the valve shaft rod 301 rotates again. At this time, the bevel gear 310 drives the edge gear 103 to rotate through the driven bevel gear 104. The edge gear 103 drives the driving gear ring 109 to rotate through the external teeth 108. Since the liquid equalizing gear 204 is arranged between the fixed gear ring 111 and the driving gear ring 109, one side of the liquid equalizing gear 204 meshes with the outer side of the fixed gear ring 111, and the other side of the liquid equalizing gear 204 meshes with the internal teeth 107 on the inner side of the driving gear ring 109. During the rotation of the driving gear ring 109, the liquid equalizing ring platform 201 on the liquid equalizing assembly 200 circulates between the outer base cover 101 and the inner base cover 110. At this time, the plurality of liquid passing straight pipes 202 on the liquid equalizing ring platform 201 circulate on the outer base cover 101. On the basis of the circulation, the liquid passing straight pipes 202 rotate secondarily again. In this way, during the reset process, synchronous circulation and synchronous secondary self-rotation actions of the plurality of liquid passing straight pipes 202 on the top of the outer base cover 101 are realized. During the circulation and secondary self-rotation actions, during the circulation, the liquid equalizing arc pipe 203 at the bottom of the liquid passing straight pipe 202 circulates and scatters the culture solution or other biological agent liquids on the ophthalmic culture cell tissue. During the secondary self-rotation of the circulation, through the self-rotation, the self-rotation of the liquid equalizing arc pipe 203 during the circulation is realized. At this time, the culture solution or other biological agent liquids can be scattered more evenly on the ophthalmic culture cell tissue, and at the same time, the uniform scattering of the culture solution or other biological agent liquids during the ophthalmic biological cell culture is accelerated.

[0023] On the basis described above, in order to cooperate with the circulation and the secondary self-rotation of the circulation of the above structure, it should be emphasized that the liquid passing cap pipe 205 is rotatably arranged on the top of the liquid passing straight pipe 202 through a bearing. The inclined liquid passing inclined pipe 206 penetrates through the arc-shaped ring chamber 116 obliquely and extends into the liquid storage cavity 117. At the same time, the arc-shaped ring chamber 116 of the present invention is rotatably arranged on the I-shaped chamber 118 through a bearing. At the same time, the liquid passing straight pipe 202 of the present invention is rotatably arranged on the liquid equalizing ring platform 201. The liquid equalizing ring platform 201 is rotatably arranged between the inner base cover 110 and the outer base cover 101 through a bearing.

[0024] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An ophthalmic biological cell culture device, comprising a culture medium cover assembly (100), characterized in that: The top of the culture medium cover assembly (100) is provided with a liquid equalizing assembly (200) and a liquid adding valve assembly (300), and a culture medium box (400) is buckled under the culture medium cover assembly (100); the liquid adding valve assembly (300) forms a self-closing and press-to-open culture liquid adding structure at the top of the culture medium cover assembly (100); during the press-to-reset process, the liquid equalizing assembly (200) forms a culture liquid equalizing structure at the top of the culture medium cover assembly (100) that moves with the ring and rotates twice.

2. An ophthalmic biological cell culture device according to claim 1, characterized in that: The culture medium cover assembly (100) comprises an outer base cover (101), the top of the outer base cover (101) is rotatably provided with a driving gear ring (109) and an edge gear (103), the top of the edge gear (103) is coaxially fixedly provided with a driven bevel gear (104), the inner side and the outer side of the driving gear ring (109) are respectively fixedly provided with inner teeth (107) and outer teeth (108), an L-shaped support arm (106) is fixedly provided at the edge of the outer base cover (101), a ring bin (105) is fixedly provided at the top of the L-shaped support arm (106), a spherical bin (112) is fixedly provided on the inner side of the ring bin (105), an industrial bin (118) and a vertical rod (119) are fixedly provided at the bottom of the industrial bin (118), and a bottom rod (119) is fixedly provided at the bottom of the industrial bin (118). 20), and an arc-shaped ring bin (116) is rotatably provided at the edge of the industrial bin (118) via a bearing, a disinfecting alcohol cotton pad (113) is provided on the inner wall of the spherical bin (112), and a liquid absorbing cotton thread (114) is provided on the outer side of the disinfecting alcohol cotton pad (113), an alcohol bin (121) is formed between the spherical bin (112) and the ring bin (105), a liquid storage cavity (117) is formed between the industrial bin (118) and the arc-shaped ring bin (116), a seat with a hole (115) is fixedly provided on both sides of the bottom of the ring bin (105), an inner base cover (110) is fixedly provided at the bottom of the bottom rod (120), a fixed tooth ring (111) is fixedly provided on the top of the inner base cover (110), and an annular groove (102) is provided between the tops of the inner base cover (110) and the outer base cover (101); The liquid equalizing component (200) comprises a liquid equalizing ring platform (201), a plurality of liquid passing straight tubes (202) are rotatably provided on the liquid equalizing ring platform (201) via bearings, a liquid equalizing arc tube (203) is fixedly provided at the bottom of the liquid passing straight tube (202), and a liquid equalizing gear (204) is fixedly provided at the top of the liquid passing straight tube (202), a liquid passing cap tube (205) is rotatably provided at the top of the liquid passing straight tube (202), and an inclined liquid passing inclined tube (206) is fixedly provided on one side of the liquid passing cap tube (205); The liquid adding valve assembly (300) comprises a valve shaft (301) and a drive end sleeve (303); a bevel gear (310) and a drive shaft (302) are fixedly provided at one end of the valve shaft (301); a liquid adding ball valve (307) is fixedly provided at the other end of the valve shaft (301); a liquid adding groove (306) is provided on the liquid adding ball valve (307); a return spring (308) is sleeved on the outside of the drive shaft (302); a drive protrusion (304) is fixedly provided on the inner wall of the drive end sleeve (303); and guide slide rods (305) are fixedly provided on both sides of the drive end sleeve (303).

3. An ophthalmic biological cell culture device according to claim 2, characterized in that: The liquid balancing ring platform (201) is rotatably arranged at the ring groove (102) between the inner base cover (110) and the outer base cover (101) via a bearing, and the liquid balancing gear (204) is arranged between the fixed gear ring (111) and the driving gear ring (109), one side of the liquid balancing gear (204) meshes with the outer side of the fixed gear ring (111), and the other side of the liquid balancing gear (204) meshes with the inner teeth (107) on the inner side of the driving gear ring (109).

4. An ophthalmic biological cell culture device according to claim 2, characterized in that: The inclined liquid-passing inclined tube (206) is fixedly arranged to penetrate the arc-shaped annular bin (116); the alcohol bin (121) is filled with disinfectant alcohol; the disinfectant alcohol cotton pad (113) siphons the disinfectant alcohol in the alcohol bin (121) onto the disinfectant alcohol cotton pad (113) through the liquid-absorbing cotton thread (114) for wetting.

5. The ophthalmic biological cell culture device according to claim 2, characterized in that: The liquid-adding ball valve (307) rotates in the spherical bin (112), the tail end of the valve shaft (301) is rotatably connected to the ring bin (105) and the spherical bin (112) via a bearing, and the driving shaft (302) and the bevel gear (310) are arranged outside the ring bin (105).

6. An ophthalmic biological cell culture device according to claim 2, characterized in that: The drive end sleeve (303) is sleeved on the drive shaft (302), the drive protrusion (304) is inserted into the drive spiral groove (309), one end of the return spring (308) abuts against the drive end sleeve (303), and the other end of the return spring (308) abuts against the outer wall of the ring chamber (105).

7. The ophthalmic biological cell culture device according to claim 2, characterized in that: The bevel gear (310) at one end of the valve shaft (301) meshes with the driven bevel gear (104), and the edge gear (103) meshes with the outer teeth (108) on the outside of the driving gear ring (109).

8. An ophthalmic biological cell culture device according to claim 2, characterized in that: The driving end sleeve (303) slides linearly and reciprocatingly on the driving shaft (302), and the guide slide bar (305) slides linearly and reciprocatingly in the perforated seat (115).

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