Detection device for colloidal gold immunochromatography of dairy products

Abstract

The invention relates to a dairy product gold-labeled immunochromatography detection device, which is characterized in that a movably placed sample rack is arranged above a swing frame, holes of the sample rack are in one-to-one correspondence with holes of the swing frame, the middle lower part of a sample tube is inserted into the holes of the swing frame, and a sampler can penetrate into a sealing film of the sample tube to input a dairy product into the sample tube; the swing frame drives the middle lower part of the sample tube to swing, so that the gold-labeled antibody and the sample are uniformly mixed; the sampler and the sealing device can move along the three-dimensional moving frame; an adsorption plate of the sealing device can absorb a sealing cover on the incubation pool and buckle the sealing cover on the top of the sample tube, and a pair of clamping forceps can clamp a sample frame into the incubation pool for incubation; a first conveying belt and a detector are arranged in the detection area, the sampler is used for dropwise adding a mixed sample solution to a detection card on the first conveying belt, and the detector is used for detecting chromatography test paper of the detection card to determine the content of a target substance in a dairy product sample. The problems that in an existing gold-labeled antibody chromatography detection card, a sample and a gold-labeled antibody need to be subjected to a heterogeneous reaction, the reaction is insufficient, and the sensitivity is not high are solved.

Description

Technical Field

[0001] The present invention belongs to the technical field of dairy gold-label immunochromatography, and particularly relates to a detection device for dairy gold-label immunochromatography. Background Art

[0002] Dairy products are an important part of people's daily diet, and the quality and safety of dairy products are very important. The detection of harmful substance targets in dairy products still mainly relies on manual operation of colloidal gold test strips. In the traditional colloidal gold detection card detection technology, the gold-labeled antibody is fixed on the release pad of the detection card. After the dairy product sample is dropped into the sample adding hole of the detection card, when the liquid flows through the release pad, it directly carries the gold-labeled antibody to chromatograph to the detection line position and reacts with the antigen to produce a color reaction. The target substance in the dairy product sample undergoes a heterogeneous reaction with the gold-labeled antibody, the reaction is not sufficient, and the sensitivity is not high. In addition, for the traditional colloidal gold detection technology, a large number of professional technical personnel are required, and the uncertainty factors introduced by manual operation may affect the accuracy of the detection results. Summary of the Invention

[0003] In view of the above problems, the present invention provides a detection device for dairy gold-label immunochromatography, which includes a three-dimensional moving frame, a sample preparation area and a detection area. The sample preparation area includes a sampler, a sealer, a swing frame and an incubation pool. A number of swing frames are arranged in a row. Above the swing frame, a sample rack that can be movably placed is provided for placing a number of sample tubes. The holes of the sample rack correspond one by one to the holes of the swing frame. The middle and lower parts of the sample tubes are inserted into the corresponding holes on the swing frame. The sampler can pierce the sealing film of the sample tube and input the dairy product sample into the sample tube. The swing frame drives the middle and lower parts of the sample tube to swing back and forth to mix the gold-labeled antibody and the sample evenly; The sampler and the sealer can move along the three-dimensional moving frame; the sealer includes an adsorption plate and a clamping pliers. The adsorption plate can suck the sealing cover on the incubation pool and buckle it on the top of the sample tube, and the clamping pliers can clamp the sample rack into the incubation pool for incubation; A first conveyor belt and a detector are provided in the detection area. The sampler sucks the mixed liquid in the incubated sample tube and drops it on the detection card on the first conveyor belt. The detector is used to detect the chromatographic test paper of the detection card to determine the content of the target substance in the dairy product sample.

[0004] Optionally, the swing frame is of a frame structure, with a number of holes provided at the top. The number of holes is evenly arranged along the length direction of the swing frame. The bottom surface of the swing frame is slidably connected to the bottom track below. A number of bottom tracks correspond to a number of swing frames and are arranged in a row; At least one driving device is provided on the side surface of the swing frame. The driving device is connected to one end of the swing frame through a rod to drive the swing frame to move back and forth along the bottom track.

[0005] Optionally, the sampler includes an air pump and a sampling part connected to each other. The air pump is connected to an external air supply device to supply air or suck air for the sampling part. The bottom opening of the sampling part is used to connect a pipette tip.

[0006] Optionally, the sample tube includes a support part and a container part. The support part is detachably mounted on the top of the container part. The support part is made of rigid plastic and is cylindrical with openings at both the top and the bottom, and is used to support the sample tube on the hole of the sample rack. The container part is made of a flexible material, such as soft rubber, and is in a conical shape with a larger top and a smaller bottom, and can be inserted into the hole of the rocking rack and be led to move by the rocking rack.

[0007] Further optionally, the gold-labeled antibody is freeze-dried and then placed into the container part. The top of the support part is heat-sealed with an aluminum film to effectively isolate the gold-labeled antibody from the moisture in the environment, which is beneficial to the long-term preservation of the gold-labeled antibody. When the pipette tip pierces the aluminum film, the dairy product sample can be input into the sample tube.

[0008] Optionally, the sealer includes an electric control device, a controller and a lifting hammer. The electric control device is electrically connected to an external power supply and an adsorption plate. After power is connected to the adsorption plate, the adsorption plate generates a magnetic suction force to attract the sealing cover to seal the sample tube. The two grippers of the clamping pliers are respectively arranged on both sides of the adsorption plate and can approach or move away from each other to clamp or release both ends of the sample rack. The lifting hammer can move along its own track and perform lifting actions at the same time. The lifting hammer strikes the adsorption plate to buckle the sealing cover on the mouth of the sample tube. The controller is connected to and controls the actions of the lifting hammer and the clamping pliers.

[0009] Further optionally, a driver is provided at the top of the lifting hammer to control the up and down movement of the lifting hammer. The driver is slidably connected to its own track, and the controller is connected to the driver to control the operation of the driver.

[0010] Optionally, the sealing cover is a hollow plastic circle. The bottom of the circle can be connected to the top of the sample tube, and the top of the circle is connected to a first fixing plate. The part of the first fixing plate corresponding to the sealing cover is hollowed out, and the first fixing plate and the sealing cover are integrally formed. A second fixing plate is provided above the first fixing plate, and a plastic film is clamped between the second fixing plate and the first fixing plate so that the plastic film can cover the mouth of the sample tube. At least one magnetic block is provided on the upper surface of the second fixing plate.

[0011] Optionally, in the detection area, a card storage bin, a first conveyor belt and a second conveyor belt are provided from top to bottom. The card storage bin is vertical and several detection cards are stacked inside. Both the first conveyor belt and the second conveyor belt are horizontal. A conveying part is provided between the first conveyor belt and the second conveyor belt for conveying the detection cards. A heating bin is provided outside the second conveyor belt for incubating the detection card with the mixed liquid dropped thereon to make it react better, and then chromatograph and develop color. A detector is provided at the tail of the second conveyor belt.

[0012] Further optionally, a plurality of positioning frames are arranged on the first conveyor belt. The positioning frames protrude from the upper surface of the first conveyor belt. The plurality of positioning frames are uniformly arranged along the moving direction of the first conveyor belt and are arranged in a straight line. The positioning frames are used to hold the test card, facilitating the sampler to determine the position of the test card. The positioning frame is a square with three sides. The opening of the positioning frame faces the opposite direction of the movement of the first conveyor belt. The opening of the positioning frame on the upper surface of the first conveyor belt faces the card storage bin. Description of the Drawings

[0013] Figure 1 is a schematic structural diagram of a detection device for dairy gold immunochromatography; Figure 2 is Figure 1 a side view schematic diagram of; Figure 3 is a schematic diagram of a sample tube; Figure 4 is a schematic diagram of a locator; Figure 5 is a schematic diagram of the cooperation of a sealing cover, a first fixing plate and a second fixing plate.

[0014] In the drawings, 1 - sampler, 2 - sealer, 3 - swing rack, 4 - heating chamber, 5 - positioning frame, 6 - sample tube, 7 - adsorption plate, 8 - clamping pliers, 9 - first conveyor belt, 10 - second conveyor belt, 11 - detector, 12 - test card, 13 - X-axis moving track, 14 - Y-axis moving track, 15 - Z-axis moving track, 16 - slide rail, 17 - telescopic rod, 18 - positioning half-frame, 19 - pipette tip, 20 - bottom track, 21 - driving device, 22 - air pump, 23 - sampling part, 24 - support part, 25 - container part, 26 - lifting hammer, 27 - outer shell, 28 - sealing cover, 29 - first fixing plate, 30 - second fixing plate, 31 - card storage bin, 32 - conveying part. Detailed Embodiment

[0015] This embodiment provides a detection device for dairy gold immunochromatography. As Figures 1-5 shown, it includes a three-dimensional moving frame, a sample preparation area and a detection area. The sample preparation area includes a sampler 1, a sealer 2, a swing rack 3 and an incubation pool. A plurality of swing racks 3 are arranged in rows. Above the swing rack 3, a movable sample rack is provided for placing a plurality of sample tubes 6. The holes of the sample rack correspond to the holes of the swing rack 3 one by one. The middle and lower parts of the sample tube 6 are inserted into the corresponding holes on the swing rack 3. The sampler 1 can pierce the sealing film of the sample tube 6 and input the dairy sample into the sample tube 6. The swing rack 3 drives the middle and lower parts of the sample tube 6 to swing back and forth to mix the gold-labeled antibody and the sample evenly. The sampler 1 and the sealer 2 can move along a three-dimensional moving rack; the sealer 2 includes a suction plate 7 and clamping pliers 8. The suction plate 7 can suck the sealing cap 28 on the incubation pool and buckle it on the top of the sample tube 6, and the clamping pliers 8 can clamp the sample rack into the incubation pool for incubation; A first conveyor belt 9 and a detector 11 are provided in the detection area. The sampler 1 sucks the mixed liquid in the incubated sample tube 6 and drops it on the test card 12 on the first conveyor belt 9. The detector 11 is used to detect the chromatographic test paper of the test card 12 to determine the content of the target substance in the dairy product sample.

[0016] The detection device of the present invention mainly completes two major functions of sample preparation and detection. Specifically, the gold-labeled antibody is put into the sample tube 6 in advance and then sealed. The sampler 1 pierces into the sample tube 6, injects the dairy product sample into the sample tube 6, and the dairy product and the gold-labeled antibody are mixed and reacted in the sample tube 6. In order to promote this reaction, the present invention designs a swing rack 3 and an incubation pool. The swing rack 3 can drive the lower part of the sample tube 6 to swing, promoting the uniform mixing of the dairy product and the gold-labeled antibody and making the reaction more sufficient. The sample tube 6 is in the incubation pool, and at a certain temperature, it can also promote the reaction.

[0017] However, before the sample tube 6 is sent into the incubation pool, it needs to be sealed again to avoid being affected by the environment. However, the sampler 1 has already pierced the seal. How to quickly and simply seal the mouth of the sample tube 6 again is a problem. The present invention designs a sealer 2, whose suction plate 7 can magnetically attract the sealing cap 28 and then buckle it on the top of the sample tube 6 to achieve simple sealing. Finally, the sampler 1 pierces the simple seal again, sucks the mixed liquid of the dairy product and the gold-labeled antibody, and then drops it onto the test card for chromatography. Finally, the detector 11 quantitatively detects the content of the target substance in the dairy product sample.

[0018] Optionally, the three-dimensional moving rack includes an X-axis moving track 13, a Y-axis moving track 14, and a Z-axis moving track 15. The X-axis moving track 13 is provided on the side of the sample preparation area and the detection area. The bottom of the Y-axis moving track 14 is slidably connected to the X-axis moving track 13. One end of the Z-axis moving track 15 is slidably connected to the Y-axis moving track 14, and the other end extends in a direction away from the X-axis moving track 13; the sampler 1 and the sealer 2 are slidably connected to the Z-axis moving track 15 and can move positions in the sample preparation area and the detection area under the control of the three-dimensional moving rack.

[0019] Optionally, the sample preparation area is divided into several areas for respectively placing a consumable rack, a dairy product original sample rack, a swing rack 3, an incubation pool, an incubated sample rack, and a waste bin. The sampler 1 and the sealer 2 move between the various areas under the control of the three-dimensional moving rack; the sample rack, the consumable rack, and the dairy product original sample rack are all conventional multi-well test tube racks or sampling tube racks. The consumable rack is used to place a plurality of pipette tips 19, the dairy product original sample rack is used to place a plurality of specimen tubes each containing a dairy product alone, and the waste bin is used to hold the used pipette tips 19.

[0020] Optionally, the swing rack 3 is a frame structure with several holes provided on the top. The several holes are evenly arranged along the length direction of the swing rack 3. The bottom surface of the swing rack 3 is slidably connected to the lower bottom track 20 below. The several bottom tracks 20 correspond to the several swing racks 3 and are arranged in a row. At least one driving device 21 is provided on the side surface of the swing rack 3. The driving device 21 is connected to one end of the swing rack 3 through a rod, and drives the swing rack 3 to move back and forth along the bottom track 20.

[0021] As a specific implementation manner, the swing rack 3 is a frame structure of a cuboid, having upper and lower layers. At least one row of holes is evenly provided on the upper top surface for inserting the middle and lower parts of the sample tube 6. The lower surface of the bottom surface of the lower layer is slidably connected to the bottom track 20. One driving device 21 is provided on the side surface of the swing rack 3. The driving device 21 is connected to one end of each swing rack 3 close to the driving device 21 through several rods in parallel. The swing rack 3 is driven to approach or move away from the driving device 21 by the telescopic movement of the rods, so as to drive the middle and lower parts of the sample tube 6 to swing back and forth, and mix the internal gold-labeled antibody with the sample evenly. According to the number of sample tubes 6, the number of swing racks 3 to be used is selected. For the temporarily unused swing racks 3, the corresponding rods are removed, that is, they are not connected to the driving device 21.

[0022] Alternatively, several driving devices 21 are provided on the side surface of the swing rack 3. The driving devices 21 are connected to one end of the swing rack 3 close to the driving device 21 through rods. The driving devices 21 correspond to the swing racks 3 one by one, and individual driving of the swing racks 3 is realized.

[0023] Optionally, the sample rack is a single-layer rack with only one horizontal plate. Several holes are evenly provided on the plate for placing the sample tubes 6. The horizontal plate is located above the swing rack 3. The holes of the sample rack and the holes of the swing rack 3 are vertically corresponding, so that the sample tubes 6 pass through the corresponding holes of the sample rack and the swing rack 3. The sample rack is placed across all the swing racks 3 and the bottom tracks 20.

[0024] Optionally, the sampler 1 includes an air pump 22 and a sampling part 23 which are connected to each other. The air pump 22 is connected to an external air supply device to supply air or suck air for the sampling part 23. The bottom opening of the sampling part 23 is used for connecting a pipette tip 19. The air pump 22 is an existing air plunger pump.

[0025] Before use, place the sample rack above the swing rack 3. Insert the required number of sample tubes 6 for detection into the corresponding holes of the sample rack and the swing rack 3. The sample tubes 6 are placed compactly, using the minimum number of swing racks 3. Then, dispense different dairy samples into the corresponding test tubes and place them on the original dairy sample rack. During use, the sampler 1 moves along the three-dimensional moving rack to above the consumable rack and then descends. A pipette tip 19 is inserted at the bottom of the sampling part 23. The sampler 1 then moves to the original dairy sample rack and aspirates the dairy under the control of the air pump 22. Then, the sampler 1 moves above the swing rack 3. When descending, the pipette tip 19 pierces the sealing film at the mouth of the sample tube 6, and the air pump 22 injects the dairy into the sample tube 6. The sampler 1 then moves to the waste bin. There is a side wall protruding inward at the top opening of the waste bin. This side wall catches the top edge of the pipette tip 19. The sampler 1 moves upward to remove the pipette tip 19, and the pipette tip 19 drops into the waste bin. After all the sample tubes 6 on one swing rack 3 are loaded, the driving device 21 of the swing rack 3 drives the swing rack 3 to move to mix the dairy and the gold-labeled antibody.

[0026] Optionally, the sample tube 6 includes a support part 24 and a container part 25. The support part 24 is detachably installed at the top of the container part 25. The support part 24 is made of hard plastic and is cylindrical with openings at both the top and bottom, and is used to support the sample tube 6 on the hole of the sample rack. The container part 25 is made of a flexible material, such as soft rubber, and is in a conical shape with a larger top and a smaller bottom, and can be inserted into the hole of the swing rack 3 and be led to move by the swing rack 3.

[0027] Further optionally, there is a circumferentially protruding part at the top of the container part 25, and there is also a circumferentially protruding part at the bottom of the support part 24. The circumferentially protruding parts of the support part 24 and the container part 25 are engaged with each other to achieve the connection between the two. After the gold-labeled antibody is freeze-dried, it is placed into the container part 25. The top of the support part 24 is heat-sealed with an aluminum film to effectively isolate the gold-labeled antibody from the moisture in the environment, which is beneficial to the long-term preservation of the gold-labeled antibody. The pipette tip 19 pierces the aluminum film, and then the dairy sample can be input into the sample tube 6.

[0028] When there are many sample tubes 6 required for one detection, the plastics at the tops of the support parts 24 of each sample tube 6 can be made to be connected, that is, the support parts 24 of the sample tubes 6 are connected into one piece, and multiple sample tubes 6 can be taken at the same time.

[0029] The traditional sample tube 6 is made of hard plastic and integrally formed, with an openable lid connected to the top, but the sealing performance of the lid is average. In order to promote the full reaction between dairy products and gold-labeled antibodies in the present invention, a certain amount of gold-labeled antibodies is freeze-dried into a lyophilized powder and placed in the sample tube 6 for storage until use during detection. However, the sealing performance of the lid of the traditional sample tube 6 cannot meet the requirements for storing the lyophilized powder. The present invention uses an aluminum film heat-sealing technology, which is applicable to the support part 24 made of plastic material, has a good sealing effect, and the sealing operation is simple and convenient.

[0030] The present invention has also considered that after the dairy products are placed in the sample tube 6, the air pump 22 is directly used for suction, so that the dairy products and the gold-labeled antibodies are mixed in the sample tube 6 and the pipette tip 19. However, this will cause the mixed liquid to vibrate violently, and it is easy to splash and cause material loss. If the unreacted dairy products or antigens are lost, it will directly lead to inaccurate quantitative detection. In addition, the mixed liquid is also easy to splash onto or stick to the sampling part 23, contaminating the next sample.

[0031] The sample tube 6 of the present invention is divided into two parts that can be disassembled up and down, with low raw material costs, simple production, and can be mass-produced. The connection and assembly of the support part 24 and the container part 25 are simple. The support part 24 provides sufficient strength for the sample tube 6 and can be snap-connected and supported on the holes of the sample rack. The container part 25 is made of a flexible material. The container part 25 is inserted into the holes of the swing rack 3 and can swing back and forth with the swing rack 3 to promote the mixing of dairy products and gold-labeled antibodies. The movement of the swing rack 3 is controlled by the driving device 21 and is controllable, avoiding violent vibration of the mixed liquid. The mixed liquid only mixes and incubates in the sample tube 6, without contaminating other components, avoiding contamination and improving the accuracy of detection.

[0032] Optionally, the sealer 2 includes an electric controller, a controller, and a lifting hammer 26. The electric controller is electrically connected to an external power supply and the adsorption plate 7. After power is supplied to the adsorption plate 7, the adsorption plate 7 generates a magnetic suction force, which can attract the sealing cover 28 to seal the sample tube 6. The two clamping jaws of the clamping pliers 8 are respectively arranged on both sides of the adsorption plate 7 and can approach or move away from each other to clamp or release the two ends of the sample rack. The lifting hammer 26 can move along its own track and perform lifting actions at the same time. The lifting hammer 26 strikes the adsorption plate 7, which can buckle the sealing cover 28 on the mouth of the sample tube 6. The controller is connected to and controls the actions of the lifting hammer 26 and the clamping pliers 8.

[0033] Further optionally, the sealer 2 includes a housing 27. One side of the housing is slidably connected to the Z-axis moving track 15. The electric controller and the controller are arranged inside the housing. The adsorption plate 7 is arranged below the housing. The clamping pliers 8 are connected to the bottom of the housing. The bottom of the housing is provided with its own track, and the lifting hammer 26 can move along the track. The adsorption plate 7 is suspended below the housing through several connecting rods.

[0034] Further optionally, the grasping forceps of the clamping forceps 8 have two position gears, one gear is close to the adsorption plate 7, and the other gear is far away from the adsorption plate 7. When both grasping forceps are close to the adsorption plate 7, the sample rack can be grasped; when both grasping forceps are far away from the adsorption plate 7, the sample rack can be released, and the distance between the two grasping forceps is greater than the length or width of the sample rack to avoid the grasping forceps touching the sample rack at this time; the bottom of the grasping forceps is lower than the adsorption plate 7.

[0035] Further optionally, the adsorption plate 7 is a flat plate, and its area is slightly smaller than the area of ​​the top surface of the sample rack, but can cover all the holes of the sample rack.

[0036] Further optionally, a driver is provided on the top of the lifting hammer 26 for controlling the lifting hammer 26 to move up and down; the driver is slidably connected to its own track, and the controller is connected to the driver to control the operation of the driver.

[0037] Optionally, the sealing cover 28 is a hollow plastic circle, the bottom of the circle can be connected to the top of the sample tube 6, and the top of the circle is connected to the fixing plate 29. The part of the fixing plate 29 corresponding to the sealing cover 28 is hollow, and the fixing plate 29 and the sealing cover 28 are integrally formed; a fixing plate 2 30 is provided above the fixing plate 29, and a plastic film is sandwiched between the fixing plate 2 30 and the fixing plate 29, so that the plastic film can cover the corresponding tube mouth of the sample tube 6; at least one magnetic block is provided on the upper surface of the fixing plate 2 30.

[0038] The second fixing plate 30, the first fixing plate 29 and the plastic film are detachable, the two fixing plates can be reused, and the second fixing plate 30, the first fixing plate 29 and the plastic film are packaged to facilitate overall movement.

[0039] During use, after the mixed liquid in the sample tube 6 is mixed, the sealer 2 moves to the top of the incubation pool, which is covered with a cover plate, on which a sealing cover 28 and two fixing plates are placed, and the adsorption plate 7 is energized to suck up the fixing plate 2 30. The sealer 2 then moves to the top of the sample rack, and the two fixing plates are covered on the top of the sample rack, and the sealing cover 28 corresponds to the top of the sample tube 6 one by one. The lifting hammer 26 moves to the position corresponding to each sample tube 6 along its own track in turn, and the lifting hammer 26 hits the adsorption plate 7 vertically downward in turn, indirectly acting on the two fixing plates and the sealing cover 28, so that the sealing cover 28 is buckled with the corresponding sample tube 6. At this time, the two fixing plates are connected to the sample tube 6 through the sealing cover 28.

[0040] The clamping forceps 8 clamp the sample rack and move it into the incubation pool (remove the cover in advance), and the sealer 2 moves out of the incubation pool. The incubation pool can provide a metal bath at a certain temperature to promote the reaction between the dairy product and the gold-labeled antibody in the sample tube 6. After incubation, the sealer 2 moves the sample rack out and places it in the sample preparation area, and the incubation pool is covered with the cover again. The fixing plate 2 30 is removed to expose the plastic film, and the sampler 1 pierces the film to sample in the sample tube 6.

[0041] Optionally, a card storage bin 31, a first conveyor belt 9 and a second conveyor belt 10 are provided in the detection area from top to bottom. The card storage bin 31 is vertical, and a number of detection cards 12 are stacked inside; both the first conveyor belt 9 and the second conveyor belt 10 are horizontal. A conveying part 32 is provided between the first conveyor belt 9 and the second conveyor belt 10 for conveying the detection cards; a heating bin 4 is provided outside the second conveyor belt 10 for incubating the detection cards with the mixed liquid dropped thereon to make them react better, and then chromatograph and develop color; a detector 11 is provided at the tail of the second conveyor belt 10.

[0042] Further optionally, the card storage bin 31 is a cuboid, with a pushing part provided at the top inside and an opening at the bottom. Manually pressing down the pushing part can push the lowermost detection card to fall from the bottom opening onto the first conveyor belt 9.

[0043] Further optionally, a number of positioning frames 5 are provided on the first conveyor belt 9. The positioning frames protrude from the upper surface of the first conveyor belt. The number of positioning frames 5 is evenly arranged along the moving direction of the first conveyor belt 9 and is arranged in a straight line. The positioning frames 5 are used to hold the detection cards to facilitate the sampler 1 to determine the position of the detection cards; The positioning frame 5 is a square with three sides. The opening of the positioning frame 5 on the upper surface of the first conveyor belt 9 faces the opposite direction of the movement of the first conveyor belt 9, and the opening of the positioning frame 5 on the upper surface of the first conveyor belt 9 faces the card storage bin 31.

[0044] Further optionally, a positioner is provided below the card storage bin 31. The positioner includes two slide rails 16 and two retractable positioning claws. The positioning claws are slidably connected to the slide rails 16. The slide rails 16 are parallel to the first conveyor belt 9. The two slide rails 16 and the positioning claws are symmetrically arranged left and right with the first conveyor belt 9 as the center line; The positioning claw includes a telescopic rod 17 and a positioning half-frame 18. The positioning half-frame 18 is connected to the slide rail 16 through the telescopic rod 17. The positioning half-frame 18 is L-shaped. The shape formed by the two symmetrical positioning half-frames 18 is the same as that of the positioning frame 5, and is used to frame the detection card that falls onto the first conveyor belt 9.

[0045] The driving principles of both the first conveyor belt 9 and the second conveyor belt 10 are of the conventional roller drive form. The running speeds of the two conveyor belts are adjustable. The first conveyor belt 9 transports the first detection card (positioned and framed by the first positioning frame 5) to the designated position, then pauses, waiting for the sampler 1 to suck the mixed liquid after incubation in the sample tube 6 and drop it into the sample addition hole of the detection card. At this time, the card storage bin 31 releases the second detection card (to be detected), and the positioning claw clamps the second detection card and moves it to the second positioning frame 5 to frame and position the second detection card. After the above operations are completed, the first conveyor belt 9 moves again, and then the second detection card is operated.

[0046] The moving form of the second conveyor belt 10 is the same as that of the first conveyor belt 9, leading the test card with the sample added to move in the heating chamber 4. (Compared with continuous movement), it can also extend the incubation time of the test card, which is beneficial to improving the accuracy of detection. The test card removed from the heating chamber 4 can be manually moved into the detector 11. The head and tail of the second conveyor belt 10 are outside the heating chamber 4, which is convenient for receiving and taking out the test card. The second conveyor belt can be arranged directly below the first conveyor belt or obliquely below the first conveyor belt.

[0047] The detector 11 uses a laser light source to emit light of a specific wavelength, irradiates the test line and quality control line areas of the test card, and then detects the intensity of the reflected light through a highly sensitive photoelectric detection component. Based on the intensity of the reflected light, it determines the color depth of the test line and quality control line, thereby making a qualitative or quantitative determination of the content of the target substance in the dairy product.

[0048] The test card released from the card storage bin 31 has its length direction basically parallel to the length direction of the first conveyor belt 9. If it is slightly inclined, it is corrected by the positioning claws. Specifically, the test card falls onto the first conveyor belt 9, and the first conveyor belt 9 pauses moving. The preset positioning frame 5 is in the front (i.e., in the front along the conveying direction of the first conveyor belt 9). The two telescopic rods 17 extend, pushing the two positioning half-frames 18 closer to each other, thereby framing the test card between the two positioning half-frames 18 (at this time, the opening of the square formed by the two positioning half-frames 18 faces the opening of the front positioning frame 5). At the same time, it corrects the length direction of the test card to be parallel to the length direction of the first conveyor belt 9. The positioning claws move along the slide rail 16 in the conveying direction of the first conveyor belt 9, thereby pushing the test card into the front positioning frame 5. At this time, the positioning claws and the positioning frame 5 form a complete square. Then, the two telescopic rods 17 contract, the two positioning half-frames 18 move away from each other, disengaging from the test card, and the positioning claws reset along the slide rail 16 to below the card storage bin 31, waiting to move the next test card.

[0049] Optionally, the conveying part 32 is an inclined downward chute, connecting the tail of the first conveyor belt 9 and the head of the second conveyor belt 10. Vertical guard plates are provided on both sides of the chute to prevent the test card from falling from the side of the chute.

[0050] Optionally, the test card is a cuboid, having a shell. Inside the shell, a sample pad, a chromatographic test paper, and a water-absorbing filter paper are provided along the length direction of the test card. The chromatographic test paper is a nitrocellulose membrane (NC membrane). A quality control line and a test line are provided on the chromatographic test paper, and it is assembled according to a conventional test card.

[0051] Further optionally, the part of the upper surface of the shell corresponding to the nitrocellulose membrane is made of transparent plastic material, which is convenient for receiving detection and at the same time avoids the influence of environmental humidity changes on the chromatographic reaction; In the middle of the part of the upper surface of the housing corresponding to the sample pad, there is a hollow hole. The side wall of the hole bulges outwards, and the top of the side wall is heat-sealed with an aluminum film. The pipette tip 19 at the bottom of the sampler 1 pierces the aluminum film heat-seal, and the mixed liquid in the tip can be dropped into the hole.

[0052] In the traditional test strip mode, the sample pad and the nitrocellulose membrane are partially exposed, which is easily affected by environmental pollution and affects the detection accuracy. The form adopted by the present invention is a test card, and the test strip is wrapped by a plastic shell to be protected from the environment.

Claims

1. A detection device for dairy product gold-label immunochromatography, characterized in that, It includes a three-dimensional moving rack, a sample preparation area and a detection area. The sample preparation area includes a sampler, a sealer, a rocking rack and an incubation pool. A number of rocking racks are arranged in a row. Above the rocking rack, there is a sample rack that can be movably placed for placing a number of sample tubes. The holes of the sample rack correspond to the holes of the rocking rack one by one. The middle and lower parts of the sample tubes are inserted into the corresponding holes on the rocking rack. The sampler can pierce the sealing film of the sample tube and input the dairy product sample into the sample tube. The rocking rack drives the middle and lower parts of the sample tubes to swing back and forth to mix the gold-labeled antibody and the sample evenly; The sampler and the sealer can move along the three-dimensional moving rack; the sealer includes an adsorption plate and clamping pliers. The adsorption plate can suck the sealing cover on the incubation pool and buckle it on the top of the sample tube. The clamping pliers can clamp the sample rack into the incubation pool for incubation; In the detection area, there is a first conveyor belt and a detector. The sampler sucks the mixed liquid in the incubated sample tube and drops it on the test card on the first conveyor belt. The detector is used to detect the chromatographic test paper of the test card to determine the content of the target substance in the dairy product sample.

2. The detection device for dairy gold-label immunochromatography according to claim 1, characterized in that, The rocking rack is a frame structure, with a number of holes provided at the top. The number of holes is evenly arranged along the length direction of the rocking rack. The bottom surface of the rocking rack is slidably connected to the bottom track below. A number of bottom tracks correspond to a number of rocking racks and are arranged in a row; At least one driving device is provided on the side of the rocking rack. The driving device is connected to one end of the rocking rack through a rod to drive the rocking rack to move back and forth along the bottom track.

3. The detection device for dairy gold-label immunochromatography according to claim 1, characterized in that, The sampler includes an air pump and a sampling part that are connected to each other. The air pump is connected to an external air supply device to supply air or suck air for the sampling part. The bottom opening of the sampling part is used to connect a pipette tip.

4. The detection device for dairy gold-label immunochromatography according to claim 3, characterized in that, The sample tube includes a support part and a container part. The support part is detachably installed on the top of the container part. The support part is made of hard plastic and is cylindrical, with openings at both the top and the bottom for supporting the sample tube on the hole of the sample rack; the container part is made of flexible material and is a conical shape with a larger top and a smaller bottom, which can be inserted into the hole of the rocking rack and be driven to move by the rocking rack.

5. The detection device for dairy gold-label immunochromatography according to claim 4, wherein, The gold-labeled antibody is freeze-dried and placed in the container part. The top of the support part is heat-sealed with an aluminum film to effectively isolate the gold-labeled antibody from the moisture in the environment; the pipette tip pierces the aluminum film and can input the dairy product sample into the sample tube.

6. The detection device for dairy gold-label immunochromatography according to claim 1, wherein, The sealer includes an electric control device, a controller and a lifting hammer. The electric control device is electrically connected to an external power supply and the adsorption plate. After the adsorption plate is powered on, the adsorption plate generates a magnetic suction force and can attract the sealing cover to seal the sample tube; The two clamping jaws of the clamping pliers are respectively arranged on both sides of the adsorption plate and can approach or move away from each other to clamp or release both ends of the sample rack; the lifting hammer can move along its own track and perform lifting actions at the same time. The lifting hammer strikes the adsorption plate to buckle the sealing cover on the mouth of the sample tube; the controller is connected to and controls the actions of the lifting hammer and the clamping pliers.

7. The detection device for dairy gold-label immunochromatography according to claim 6, wherein, A driver is provided at the top of the lifting hammer for controlling the up and down movement of the lifting hammer; the driver is slidably connected to its own track, and the controller is connected to the driver to control the operation of the driver.

8. The detection device for dairy gold-label immunochromatography according to claim 6, wherein, The sealing cap is a hollow plastic circle. The bottom of the circle can be connected to the top of the sample tube, and the top of the circle is connected to the first fixing plate. The part of the first fixing plate corresponding to the sealing cap is hollowed out, and the first fixing plate and the sealing cap are integrally formed; there is a second fixing plate above the first fixing plate, and a plastic film is sandwiched between the second fixing plate and the first fixing plate so that the plastic film can cover the mouth of the sample tube; there is at least one magnet on the upper surface of the second fixing plate.

9. The detection device for dairy gold-label immunochromatography according to claim 1, wherein, In the detection area, there is a card storage bin, a first conveyor belt and a second conveyor belt from top to bottom. The card storage bin is vertical and several test cards are stacked inside; both the first conveyor belt and the second conveyor belt are horizontal, and there is a conveying part between the first conveyor belt and the second conveyor belt for conveying the test cards; the outside of the second conveyor belt is covered with a heating bin for incubating the test cards dropped with the mixed solution to make them react better, and then chromatograph and develop color; there is a detector at the tail of the second conveyor belt.

10. The detection device for dairy gold-label immunochromatography according to claim 9, wherein A number of positioning frames are arranged on the first conveyor belt. The positioning frames protrude from the upper surface of the first conveyor belt. The number of positioning frames is evenly arranged along the moving direction of the first conveyor belt and is arranged in a straight line. The positioning frames are used to hold the test cards to facilitate the sampler to determine the position of the test cards. The positioning frame is a square with three sides, and the opening of the positioning frame faces the opposite direction of the movement of the first conveyor belt.

Images